Use of quinic acid in the preparation of plant bioregulators

Abstract

The present application relates to the application of quinic acid in plant flowering regulation, and belongs to the field of agricultural technology. The compound is screened from normal and early bolting angelica leaf by metabolomics technology, and it is found that quinic acid has a bidirectional regulation effect of low concentration promotion and high concentration inhibition on plant flowering. Within a certain concentration range (20-200 muM), quinic acid regulates flowering of arabidopsis, and flowering phenomenon appears 2-3 days earlier than the blank group, but high concentration (1000 muM) has an inhibitory effect on flowering of arabidopsis, and flowering is delayed by about 2 days compared with the blank group. The screening of homozygous quinic acid synthesis key gene QDH mutant strain delays flowering of arabidopsis (2-3 days) in different degrees compared with the wild type. 100 muM quinic acid significantly regulates flowering of coriander 8-12 days in advance. Compared with the early bolting rate of the blank group, the early bolting rate of 100 muM quinic acid is increased by 30-40%. The effect of the disclosed quinic acid on plant flowering regulation can be used for preparing a plant flowering regulator for application and regulating the flowering period of plants.

Description

Technical Field

[0001] This invention relates to quinic acid, a compound that regulates flowering in plants, specifically a compound that can regulate flowering in Arabidopsis thaliana (a cruciferous plant), Coriander (an umbellatus plant), and Angelica sinensis (an umbellatus plant). This compound can be used for regulating flowering and controlling flowering time in plants, and belongs to the field of agricultural technology. Background Technology

[0002] A plant's life cycle mainly consists of five stages: seedling stage, growth stage, flowering stage, fruiting stage, and withering stage. Bolting and flowering, as the transition from vegetative to reproductive growth, are indispensable processes for the reproduction and evolution of flowering plants. Appropriate flowering time is a crucial prerequisite for successful seed production and propagation. To ensure successful reproduction and better survival adaptability, plants have evolved complex regulatory mechanisms to respond to external environmental disturbances and changes in their own metabolism. On the one hand, external environmental factors such as low temperature, light duration, and drought affect plant flowering; on the other hand, endogenous signals within the plant, such as hormones, sucrose, and developmental stage, also alter the flowering process.

[0003] Since the discovery of auxin in 1928, plant hormone research has been one of the most cutting-edge and hottest areas in plant science. As trace signaling molecules within plants, they play a vital role in regulating various plant growth and development processes, the accumulation of secondary metabolites, and environmental responses. Plant hormones are mainly produced by the plant's own metabolism and participate in regulating plant growth. Plant growth regulators, on the other hand, are mostly artificially synthesized and have functions similar to plant hormones. They have significant regulatory effects on plant physiological processes, such as promoting cell elongation and division, breaking seed dormancy, and promoting vegetative and reproductive growth. Based on their different physiological functions, they are mainly divided into plant growth promoters, plant growth inhibitors, and plant growth retardants. With the continuous advancement of research, some small molecule compounds produced by plant metabolism, such as proline, calcigenin, and melatonin, have also been proven to have hormone-like effects, participating as signaling molecules in regulating processes such as flowering, seed germination, and fruit ripening.

[0004] Angelica sinensis, a perennial plant that thrives in low-temperature, long-day conditions, typically has a three-year growth cycle in actual production. In normal medicinal Angelica sinensis, the roots rapidly swell after transplanting seedlings in the second year, accumulating a large amount of secondary metabolites. However, during the same period, early-bolting Angelica sinensis plants do not undergo sufficient growth and directly enter the bolting, flowering, and seed-setting stage, resulting in root decay and rendering them unsuitable for medicinal use. Early bolting is a phenomenon caused by the premature initiation of flower bud differentiation in Angelica sinensis due to the synergistic effect of environmental factors and its own development. Flower bud differentiation is a morphological marker indicating the transition from vegetative to reproductive growth in plants; generally, the earlier flower bud differentiation begins, the earlier the flowering time. By analyzing and screening the metabolites produced during the early bolting process of Angelica sinensis, we can identify new compounds with hormone-like effects for application in plant growth and development regulation, enriching the variety of plant growth regulators and better serving the flowering regulation of horticultural plants. Summary of the Invention

[0005] Purpose of the invention: The purpose of this invention is to provide quinic acid, a plant endogenous compound that regulates flowering in plants. Quinic acid plays a regulatory role in the growth of Arabidopsis thaliana, coriander, and angelica, and can serve as a novel plant bioregulator.

[0006] Technical solution: To achieve the above objectives, the technical solution adopted by this invention is as follows:

[0007] The present invention provides quinic acid, a compound that regulates flowering in plants, which was found in the leaves of normal and early-flowering Angelica sinensis.

[0008] The specific steps for quinic acid screening provided by this invention are as follows:

[0009] a) Extraction: Fresh Angelica sinensis leaves were chopped (particle size <0.5cm), and an appropriate amount of leaves was weighed and placed in a 100mL stoppered conical flask. Ten times the volume (w / v) of petroleum ether (30-60℃), ethyl acetate, methanol, methanol containing 1% formic acid, and water were added sequentially for ultrasonic extraction twice, 10 min each time. The filtrates were combined and centrifuged to dryness to obtain the sample. For samples extracted with ethyl acetate, methanol, or methanol containing 1% formic acid, the sample was dissolved in methanol; for samples extracted with water, the sample was dissolved in ultrapure water. After adding the solvent, the mixture was vortexed and ultrasonicated at room temperature (400W, 40kHz) for 10 min. The sample was then centrifuged at 13000 r / min for 10 min, and the supernatant was used for subsequent analysis.

[0010] b) Testing: Chromatographic conditions: Acquity UPLC BEH-C18 column (2.1 mm × 100 mm, 1.7 μm); mobile phase: 0.1% formic acid water (A) - acetonitrile (B); LTQ-Orbitrap Velos mass spectrometer detector.

[0011] c) Analysis: SIEVE 2.0 software was used to process the data for noise filtering, overlapping peak resolution, peak alignment, peak matching, standardization, and normalization. The data were then imported into SIMCA-P software. Principal component analysis (PCA) was used for pattern recognition analysis, and partial least-sequares discriminant analysis (PLS-DA) was used to select characteristic variables that distinguish each group. Potential biomarkers, quinic acid, were screened based on VIP>1 and p<0.05.

[0012] The study to verify the function of quinic acid in regulating flowering in plants was conducted in the model plant Arabidopsis thaliana, and included the following steps:

[0013] a) Seed disinfection and cultivation: Take an appropriate amount of Col-0 Arabidopsis thaliana seeds and disinfect them sequentially with 75% ethanol (v / v) and 5% sodium hypochlorite, followed by repeated rinsing with sterile water. The amount of solvent used during disinfection should be sufficient to completely submerge the seeds. Shake the seeds frequently during disinfection and rinsing to ensure thorough sterilization and cleaning, preventing disinfectant residue that could affect seed germination. Place the high-temperature sterilized 1 / 2 MS medium and pipette tips in a clean bench, then sow the sterilized seeds into the 1 / 2 MS medium, 15-20 seeds per bottle, ensuring even distribution and appropriate density to facilitate later growth and transplanting.

[0014] b) Seedling Transplanting and Quinic Acid Treatment: When Arabidopsis seedlings reached the 4-6 leaf stage, seedlings with relatively uniform growth were carefully removed from the culture medium using tweezers. The culture medium was removed from the roots, and the seedlings were transplanted into prepared substrate soil. The substrate soil was a mixture of peat moss, vermiculite, and perlite in a 1:1:1 mass ratio, sterilized at high temperature. After cooling, the soil was moistened, divided into culture pots, and watered lightly until just enough to seep out of the bottom of the pot. Initially, the culture pots were covered with plastic wrap. When the leaves showed significant elongation and the stems began to develop, the plastic wrap was removed, and the plants were allowed to adapt for 5-7 days before undergoing an exogenous quinic acid induction experiment. The same volume of water was applied weekly until the plants fully flowered. The growth status, bolting and flowering time, and number of rosette leaves were observed in different treatment groups.

[0015] c) Homozygous screening and flowering performance analysis of QDH mutant lines: PCR technology was used to screen Arabidopsis mutants of the key gene QDH in the quinic acid synthesis pathway. All mutant lines were in the Col-0 ecotype. The growth status, bolting and flowering time, and number of rosette leaves of different mutant lines were observed and recorded.

[0016] A study verifying the function of quinic acid in regulating plant flowering was conducted in the umbelliferous plant *Coriandrum sativum*, and included the following steps:

[0017] a) Seed disinfection and cultivation: Since coriander seeds have hard outer shells, they should be rubbed open beforehand to crack the shells. Then, soak them in warm water at approximately 40°C for 6 hours. After soaking, disinfect the seeds sequentially with 75% ethanol and 0.1% mercuric chloride, followed by repeated rinsing with sterile water. The amount of solvent used during disinfection should be enough to completely submerge the seeds. Shake the seeds frequently during disinfection and rinsing to ensure thorough sterilization and cleaning, preventing disinfectant residue that could affect seed germination. Place the sterilized MS medium and pipette tips in a clean bench, then sow 10 sterilized seeds into each MS medium, ensuring even distribution and appropriate spacing to facilitate later growth and transplanting.

[0018] b) Seedling Transplanting and Quinic Acid Treatment: When the coriander seedlings reached the 4-leaf stage, use tweezers to carefully remove seedlings with relatively uniform growth from the culture medium, remove the culture medium from the roots, and transplant them into prepared substrate soil. The substrate soil is a mixture of peat moss, vermiculite, and perlite in a 1:1:1 mass ratio, sterilized at 121℃ for 15 minutes. After cooling, the soil is moistened, divided into culture pots, and watered lightly until just enough to seep out of the bottom of the pot. In the initial stage after transplanting, cover the culture pots with plastic wrap for 3 days. After 5-7 days of acclimatization, conduct an exogenous quinic acid induction experiment. Water once a week with the same volume until the plants fully flower. Observe the growth status, bolting and flowering time, and the number of flowering rosette leaves of different treatment groups.

[0019] A study on the regulation of flowering function of quinic acid in plants was conducted on Angelica sinensis, a plant of the Apiaceae family. The study included the following steps: setting up a suitable experimental field, transplanting Angelica sinensis with the same growth status, and spraying exogenous quinic acid one week after the seedlings turned green again, once every two weeks for a total of three times, and counting the early bolting rate of Angelica sinensis.

[0020] The quinic acid obtained by screening in this invention can be used to regulate the early flowering of various plants such as Arabidopsis thaliana, coriander, and angelica. By spraying an appropriate concentration of quinic acid solution exogenously, co-culturing with Arabidopsis thaliana, coriander, angelica, and other plants is carried out.

[0021] Beneficial effects: Compared with existing research, the present invention has the following advantages:

[0022] The quinic acid discovered in this invention is an endogenous plant component and has been shown to regulate the early flowering of plants such as Arabidopsis thaliana, coriander, and angelica, as well as to delay the flowering cycle of QDH gene-mutated Arabidopsis thaliana. The effect can be obtained without the addition of adjuvants and can be used to develop green and low-cost plant flowering regulators. Attached Figure Description

[0023] Figure 1 PCA score images of Angelica sinensis leaves under positive ion mode in normal and early flowering conditions (AD: ethyl acetate; methanol; formic acid methanol; water);

[0024] Figure 2 PCA score diagrams of Angelica sinensis leaves under negative ion mode in normal and early flowering conditions (AD: ethyl acetate; methanol; formic acid methanol; water);

[0025] Figure 3 Analysis of flowering time and rosette number in Arabidopsis thaliana after exogenous quinic acid spraying;

[0026] Figure 4 Flowering phenotype of the QDH mutant Arabidopsis thaliana;

[0027] Figure 5 Screening for QDH homozygous mutant Arabidopsis thaliana;

[0028] Figure 6 Flowering phenotype of QDH homozygous mutant after exogenous quinic acid supplementation;

[0029] Figure 7 The flowering phenotype of coriander after exogenous application of quinic acid. Detailed Implementation Plan

[0030] The present invention can be better understood from the following embodiments. However, those skilled in the art will readily understand that the specific fermentation and culture processes and functional evaluations described in the embodiments are for illustrative purposes only and should not, and will not, limit the invention as described in detail in the claims.

[0031] The chemical reagents used in the following examples are all available from commercially available sources.

[0032] Example 1

[0033] Quinic acid, an endogenous plant compound with flowering-regulating function, was discovered through the following method:

[0034] (1) Extraction: Fresh Angelica sinensis leaves were chopped (particle size <0.5cm), and an appropriate amount of leaves was weighed and placed in a 100mL stoppered conical flask. Ten times the volume (w / V) of petroleum ether (30-60), ethyl acetate, methanol, 1% formic acid methanol, and water were added sequentially, and the mixture was ultrasonically extracted twice, 10 min each time. The filtrates were combined, and the filtrates were centrifuged and concentrated to dryness to obtain the sample. For samples extracted with ethyl acetate, methanol, or formic acid methanol solvents, the sample was dissolved in methanol; for samples extracted with water solvents, the sample was dissolved in ultrapure water. After adding the solvent, the mixture was vortexed and ultrasonicated at room temperature (400W, 40kHz) for 10 min. The sample was then removed, centrifuged at 13000r / min for 10 min, and the supernatant was used for subsequent analysis.

[0035] (2) Test: Chromatographic conditions: Acquity UPLC BEH-C18 column (2.1mm×100mm, 1.7μm); mobile phase: 0.1% formic acid water (A)-acetonitrile (B); LTQ-Orbitrap Velos mass spectrometer detector.

[0036] (3) Analysis: The data were processed by SIEVE 2.0 software for noise filtering, overlapping peak resolution, peak alignment, peak matching, standardization and normalization. The data were then imported into SIMCA-P software. Principal component analysis (PCA) was used for pattern recognition analysis. Partial least-sequares discriminant analysis (PLS-DA) was used to select characteristic variables that distinguish each group. Potential biomarkers, quinic acid, were obtained by screening based on VIP>1 and p<0.05.

[0037] Table 1 Sample Information

[0038] MZC2 normal group Normal two-year-old Angelica plants MZTH Early flowering period group Early bolting of angelica plants during their flowering period (two years old) MZTZ Early flowering stage group Early bolting of angelica plants during the seed-bearing stage (two years old) MZC3 Seed preservation group Three-year seed-producing Angelica plants

[0039] Example 2

[0040] Quinic acid's ability to regulate early flowering in Arabidopsis thaliana was verified through the following methods:

[0041] (1) Seed disinfection and culture: Take an appropriate amount of Col-0 Arabidopsis thaliana seeds, disinfect them successively with 75% ethanol (v / v) and 5% sodium hypochlorite, and then rinse them repeatedly with sterile water. The amount of solvent used during the disinfection process should be enough to completely submerge the seeds. Shake the seeds during disinfection and rinsing to ensure thorough sterilization and cleaning, and to prevent disinfectant residue from affecting seed germination. Place the 1 / 2MS medium and pipette tips that have been sterilized at high temperature into a clean bench, and then sow the sterilized seeds into the 1 / 2MS medium, 15-20 seeds per bottle, ensuring that the seeds are evenly distributed and appropriately spaced to facilitate later growth and transplanting.

[0042] (2) Seedling Transplanting and Quinic Acid Treatment: When Arabidopsis seedlings reached the 4-6 leaf stage, seedlings with relatively uniform growth were carefully removed from the culture medium using tweezers. The culture medium on the roots was removed, and the seedlings were transplanted into prepared substrate soil. The substrate soil was prepared by mixing peat moss, vermiculite, and perlite in a 1:1:1 ratio and sterilizing at high temperature. After cooling, the soil was moistened, and the seedlings were divided into culture pots. Water was then applied until the water just seeped out of the bottom of the pot. In the early stage of seedling transplantation, the culture pots were covered with plastic wrap. When the leaves showed obvious elongation and the stems began to develop, the plastic wrap was removed. After 5-7 days of adaptive growth, an exogenous quinic acid (QA) induction experiment was conducted. The seedlings were watered once a week with the same volume until the plants were fully flowering. The growth status, bolting and flowering time, and number of flowering rosette leaves of different treatment groups were observed.

[0043] Quinic acid (QA) at concentrations of 0-1 mM did not cause serious harm to the growth and development of Arabidopsis thaliana, and no wilting or death was observed in the plants. The results of statistical analysis on flowering time and the number of rosette leaves are as follows: Figure 3 As shown, with water treatment as a blank control, quinic acid within a certain concentration range (20-200 μM) regulated the flowering of Arabidopsis thaliana, and the flowering of Arabidopsis thaliana appeared about 2-3 days earlier than the blank group (P<0.05). However, high concentration (1000 μM, 53.54) had an inhibitory effect on the flowering of Arabidopsis thaliana, and the flowering was delayed by about 1.5 days compared with the blank group. The number of rosette leaves at the time of flowering showed an opposite trend to the plant development status.

[0044] Example 3

[0045] The effect of quinic acid on the flowering phenotype of Arabidopsis QDH mutant plants was detected by the following method:

[0046] Seeds from Arabidopsis mutant plants QDH1, QDH2, and QDH3 were collected and disinfected with 75% ethanol (v / v) for 5 min, followed by 5% sodium hypochlorite for 5 min. The seeds were then rinsed repeatedly with sterile water 4-6 times, 1 min each time. Afterward, 15-20 seeds were sown into 1 / 2 MS medium, ensuring even distribution and appropriate density to facilitate later growth and transplanting. When the Arabidopsis seedlings reached the 4-6 leaf stage, approximately 2-3 weeks after formal culture, seedlings with relatively uniform growth were carefully removed from the medium using tweezers. The medium was removed from the roots and transplanted into prepared substrate soil. The substrate soil consisted of peat moss, vermiculite, and perlite mixed in a 1:1:1 ratio and sterilized at 121℃ for 15 min. The growth status, flowering time, and number of rosette leaves of the Arabidopsis QDH mutants were observed and recorded after transfer to the substrate soil. Results are as follows: Figure 5 As shown: the QDH mutant strain does not affect the normal growth of Arabidopsis thaliana, and the plants do not wilt or die. All three mutant lines delay the flowering time of the plants.

[0047] Table 2. Information related to QDH mutant lines

[0048] QDH1 AT3G06350 T-DNA N686847 SALK_135524C QDH2 AT3G06350 T-DNA N625189 SALK_125189 QDH3 AT3G06350 T-DNA N689689 SALK_201929C

[0049] Example 4

[0050] This invention relates to homozygous Arabidopsis QDH mutant plants, which are screened using the following method:

[0051] (1) Primer design: Using the primer sequences provided on the T-DNA Primer Design website (http: / / signal.salk.edu / tdnaprimers.html), a three-primer method of upstream sequence primer (LP) + downstream primer (RP) + universal primer (BP, ATTTTGCCGATTTCGGAAC) was used for screening; T-DNA insertion direction was obtained using T-DNA Express (http: / / signal.salk.edu / cgi-bin / tdnaexpress), and a two-primer method of universal primer (BP) + upstream sequence primer (LP) / downstream sequence primer (RP) was used for screening.

[0052] (2) Homozygous screening: Total plant DNA was extracted using the CTAB method, and the DNA concentration and purity were determined using DS11. Samples with an A260 / A280 value of approximately 1.8 were retained for subsequent amplification reactions. Amplification conditions: 94℃ pre-denaturation for 5 min, 94℃ denaturation for 30 s, 53℃ annealing for 30 s, 72℃ extension for 60 s, 35 cycles, and a final extension at 72℃ for 7 min. After PCR, the samples were removed, and the amplification results were checked by agarose gel electrophoresis.

[0053] Table 3 Primer sequences for QDH mutant lines

[0054]

[0055] Example 5

[0056] The flowering phenotype of homozygous QDH Arabidopsis mutants was studied using the following methods:

[0057] Seeds from the homozygous plants QDH1 and QDH3 selected in Example 3 were disinfected with 75% ethanol (v / v) for 5 min, then with 5% sodium hypochlorite for 5 min, and then rinsed repeatedly with sterile water 4-6 times, 1 min each time. The sterilized seeds were then sown into 1 / 2 MS medium, 15-20 seeds per bottle, ensuring even distribution and appropriate density to facilitate later growth and transplanting. When the Arabidopsis seedlings reached the 4-6 leaf stage, approximately 2-3 weeks after formal culture, the seedlings with relatively uniform growth were carefully removed from the medium with tweezers, the medium was removed from the roots, and they were transplanted into prepared substrate soil. The substrate soil was prepared by mixing peat moss, vermiculite, and perlite in a 1:1:1 ratio and sterilized at 121°C for 15 min. The growth status, flowering time, and number of rosette leaves of the Arabidopsis QDH mutants were observed and recorded after transfer to the substrate soil. After three generations of homozygous selection, flowering phenotypes were tested on the homozygous lines QDH1 and QDH3, and gibberellin and quinic acid replacement experiments were also conducted. The results showed that ( Figure 6 Compared with the wild-type Col-0 (48.67±3.38), QDH1 (52.17±2.26) and QDH3 (51.46±3.05) delayed flowering in Arabidopsis thaliana to varying degrees. Exogenous addition of gibberellin and quinic acid (QA) could compensate for the delayed flowering in the QDH mutants.

[0058] This example demonstrates that QDH is a precursor enzyme for quinic acid synthesis. QDH mutants lacking quinic acid experience delayed flowering, while the addition of quinic acid advances flowering, proving that quinic acid can advance the flowering of QDH mutants.

[0059] Example 6

[0060] The function of quinic acid in regulating the early flowering of coriander, as specified in this invention, was verified by the following method:

[0061] (1) Seed disinfection and cultivation: Since coriander seeds have hard shells, they should be rubbed open beforehand to crack the shells, and then soaked in warm water at about 40℃ for 6 hours. After soaking, the seeds are disinfected with 75% ethanol and 0.1% mercuric chloride in sequence, and then rinsed repeatedly with sterile water. The amount of solvent used during the disinfection process should be enough to completely submerge the seeds. During disinfection and rinsing, the seeds should be shaken to ensure thorough sterilization and rinsing, and to prevent disinfectant residue from affecting seed germination. The MS medium and pipette tips that have been sterilized at high temperature are placed in a clean bench, and then the sterilized seeds are sown into the MS medium, 10 seeds per bottle, ensuring that the seeds are evenly distributed and appropriately spaced to facilitate later growth and transplanting.

[0062] (2) Seedling Transplanting and Quinic Acid Treatment: When the coriander seedlings reached the 4-leaf stage, the seedlings with relatively uniform growth were carefully removed from the culture medium using tweezers. The culture medium on the roots was removed, and the seedlings were transplanted into the prepared substrate soil. The substrate soil was prepared by mixing peat moss, vermiculite, and perlite in a 1:1:1 ratio and sterilizing at 121℃ for 15 minutes. After cooling, the soil was moistened, and the seedlings were divided into culture pots. Water was then applied until the water just seeped out of the bottom of the pot. The culture pots were covered with plastic wrap for 3 days during the initial transplanting period. After 5-7 days of adaptive growth, an exogenous quinic acid induction experiment was conducted. The seedlings were watered once a week with the same volume of water until the plants were fully flowering. The growth status, bolting and flowering time, and number of flowering rosette leaves of different treatment groups were observed.

[0063] Using water treatment as a blank control, 100 μM gibberellin (GA) and 100 μM quinic acid (QA) were applied. After treatment, plant height and flowering status of coriander were recorded weekly. The results showed that ( Figure 7 Compared with the control group (132.03±5.62), GA (118.7±3.14) and QA (122.73±4.59) significantly accelerated the flowering process of coriander. In addition, GA and QA treatments significantly increased the plant height of coriander. In the first three weeks of induction treatment, there were no significant differences between different groups. From the fourth week onwards, GA and QA treatments began to promote the growth of coriander plant height.

[0064] Example 7

[0065] Quinic acid's ability to regulate the early flowering of Angelica sinensis was verified through the following methods:

[0066] A suitable experimental field was set up, and angelica seedlings with optimal root irrigation treatment were selected. Angelica seedlings with consistent growth were transplanted. One week after the seedlings began to green up, quinic acid was applied exogenously. The control group received 500 mL of quinic acid per row. The concentrations of low-dose quinic acid (QAL) were 100 μM, medium-dose quinic acid (QAM) were 500 μM, and high-dose quinic acid (QAH) were 1000 μM. This was repeated every two weeks for a total of three times. The effects on bolting, flowering, and quality formation of angelica were monitored. The early bolting rate in the control group was 17.58%, while the early bolting rates for the low, medium, and high-dose quinic acid treatments were 24.22%, 33.59%, and 16.41%, respectively.

[0067] The above results indicate that this invention screened and obtained quinic acid, a plant endogenous component with flowering regulation function, and established a method for verifying its flowering function by co-culturing with plants such as Arabidopsis thaliana, coriander, and angelica. The invention also clarified the flowering delay phenomenon in the QDH mutant strain, a key gene for quinic acid synthesis, which has important practical application value for the subsequent development and preparation of plant growth regulators.

[0068] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. Application of quinic acid in the preparation of bioregulators that advance the flowering cycle of Arabidopsis thaliana or coriander or delay the flowering cycle of QDH gene-mutated Arabidopsis thaliana. When used for root irrigation, the concentration of quinic acid is 20-200 µM, and the frequency of quinic acid irrigation is 1-2 times per week.

2. Application of quinic acid in the preparation of bioregulators that promote early flowering of Angelica sinensis: Quinic acid is used for foliar spraying at a concentration of 100-1000 µM; the frequency of quinic acid spraying on plants is once every 2-3 weeks.

3. The application according to claim 1 or 2, characterized in that, The dosage form of the regulator is selected from any one of the following: powder, granules, dispersible tablets, sustained-release agents, aqueous solutions, soluble gels, oils, water emulsions, oil emulsions, and ointments.

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