Pharmaceutical preparation for sun rose and sun rose planting method

Abstract

The present application belongs to the technical field of grape cultivation, and particularly relates to a medicine preparation for sunlight rose and a sunlight rose planting method. In the present application, 5-amino levulinic acid and gibberellin A3 are jointly prepared into a medicine preparation, which can be applied to the fruit clusters of sunlight rose in the young fruit stage, so as to improve the fruit size, soluble solid content in the fruit of sunlight rose, and reduce the titratable acid content in the fruit of sunlight rose.

Description

Technical Field

[0001] This invention belongs to the field of grape cultivation technology, specifically relating to pharmaceutical preparations for Shine Muscat grapes and methods for cultivating Shine Muscat grapes. Background Technology

[0002] Shine Muscat grapes are a hybrid of European and American varieties, formed by crossing the parent 'Anyunjin 21' and 'Bainan'. Their berries are crisp and fragrant, making them widely popular with consumers. Because grape inflorescences are paniculate and naturally quite long and large, pruning is necessary to obtain neat, attractive bunches with uniform berry size. Removing excess buds also reduces nutrient waste, promotes bud development, and minimizes flower and fruit drop.

[0003] Common flower spike management measures fall into two categories. The first is manual pruning, which involves thinning out the flower spikes and berries, removing excess flowers, weak, small, and diseased berries to ensure a beautiful spike shape and uniform berry size. The second is treating the flower spikes with plant growth regulators. Common plant growth regulators include gibberellin A3 (GA3) and forchlorfenuron (CPPU). GA3 treatment during full bloom can induce seedlessness and promote flower and fruit retention, while CPPU treatment during the fruit enlargement stage increases berry size. Combining both treatments provides multiple benefits, including inducing seedlessness, retaining flowers and fruit, increasing berry size, and improving soluble solids content in the fruit. Summary of the Invention

[0004] Based on this, the present invention provides a pharmaceutical preparation for Shine Muscat roses and a method for cultivating Shine Muscat roses, thereby solving at least one problem in the prior art.

[0005] In a first aspect, the present invention provides a pharmaceutical formulation for Sunshine Rose, comprising 5-aminolevulinic acid (ALA), gibberellin A3 (GA3) and a diluent, wherein the concentration of 5-aminolevulinic acid is 80-100 mg / L and the concentration of gibberellin A3 is 20-30 mg / L.

[0006] In this invention, 5-aminolevulinic acid and gibberellin A3 are formulated together into a pharmaceutical preparation that can be applied to the fruit clusters of Shine Muscat during the young fruit stage to increase the fruit size, soluble solids content, and reduce the titratable acid content in Shine Muscat fruit.

[0007] In some preferred embodiments, the concentration of 5-aminolevulinic acid is 100 mg / L, and the concentration of gibberellin A3 is 25 mg / L.

[0008] In some preferred embodiments, the diluent is water.

[0009] Secondly, the present invention provides a method for cultivating Shine Muscat roses, which includes the following steps: During the peak blooming season of Shine Muscat, apply clean water to the fruit clusters of Shine Muscat. During the young fruit stage of Shine Muscat, the aforementioned pharmaceutical preparation for Shine Muscat is applied to the fruit clusters of Shine Muscat.

[0010] In some preferred embodiments, the water is applied to the clusters of Shine Muscat grapes by spraying or dipping the clusters, and the pharmaceutical preparation for Shine Muscat grapes is applied to the clusters by spraying or dipping the clusters.

[0011] In some preferred embodiments, the method of applying water to the fruit clusters of Shine Muscat is called spraying the clusters, and the method of applying the pharmaceutical preparation for Shine Muscat to the fruit clusters of Shine Muscat is called dipping the clusters.

[0012] In some preferred embodiments, the peak flowering period refers to May 1 to May 7.

[0013] In some preferred embodiments, the young fruit period refers to May 7 to May 20.

[0014] In some preferred embodiments, water is applied to the clusters of Shine Muscat grapes before 10:00 AM or after 4:00 PM (Beijing time).

[0015] In some preferred embodiments, the pharmaceutical preparation for Sunshine Rose is applied to the Sunshine Rose before 10:00 AM or after 4:00 PM (Beijing time).

[0016] Due to the adoption of the above technical solutions, the embodiments of the present invention have at least the following beneficial effects: Experiments were designed on the timing and method of inflorescence treatment, as well as the effects of ALA alone or in combination with traditional plant growth regulators GA3 and CPPU on the quality improvement of Shine Muscat grapes. This provides a basis for ALA treatment to improve the quality of Shine Muscat grapes and offers a new method for grape cultivation, quality improvement, and efficiency enhancement. Spraying grafted Shine Muscat grape seedlings (Beda rootstock) with water during the peak flowering period each year, and then treating them with 25 mg / L GA3 + 100 mg / L ALA during the young fruit stage, can effectively increase the weight of grape bunches, increase the longitudinal and transverse diameters of the fruit and soluble solids, reduce titratable acid content, increase the solid-acid ratio, improve fruit flavor, and enhance fruit quality. Attached Figure Description

[0017] Figure 1 This is a diagram illustrating the operation of workers processing fruit ears in an embodiment of the present invention.

[0018] Figure 2 This is a picture of the fruit ears after workers have processed them in an embodiment of the present invention.

[0019] Figure 3 This is one of the fruit ear weighing diagrams in the embodiments of the present invention.

[0020] Figure 4 This is the second weighing diagram of the ear of fruit in an embodiment of the present invention. Detailed Implementation

[0021] The following will provide a clear and complete description of the concept and technical effects of the present invention, so as to fully explain the purpose, solution and effects of the present invention.

[0022] In the following tests, 5-aminolevulinic acid solution is a solution formed by 5-aminolevulinic acid and water, and 100 mg / L of 5-aminolevulinic acid solution is expressed as 100 mg / L ALA. Gibberellin A3 solution is a solution formed by gibberellin A3 and water, and 25 mg / L of gibberellin A3 solution is expressed as 25 mg / L GA3. Clopidogrel solution is a solution formed by clopidogrel and water, and 3 mg / L clopidogrel solution is expressed as 3 mg / L CPPU. A pharmaceutical preparation is formed by mixing 25 mg / L gibberellin A3 solution and 100 mg / L 5-aminolevulinic acid solution at a volume ratio of 1:1, expressed as 25 mg / L GA3 + 100 mg / L ALA. A mixture of 25 mg / L gibberellin A3 solution, 3 mg / L chlorpyrifos solution, and 100 mg / L 5-aminolevulinic acid solution in a volume ratio of 1:1:1 is expressed as 25 mg / L GA3 + 3 mg / L CPPU + 100 mg / L ALA. A mixture of 25 mg / L gibberellin A3 solution and 3 mg / L chlorpyrifos solution in a volume ratio of 1:1 is expressed as 25 mg / L GA3 + 3 mg / L CPPU. Gibberellin A3 and chlorpyrifos were purchased from Shanghai Lanji Technology Development Co., Ltd., and 5-aminolevulinic acid was purchased from Nanchang Yingjun Technology Development Co., Ltd., all of which were of analytical grade (above 98%). Spraying and immersion of the fruit bunches were both carried out before 10:00 AM. Spraying involved spraying the solution or water onto the bunches using a spray bottle, while immersion involved immersing the bunches in a measuring cup containing the solution or water.

[0023] The experimental site was a vineyard within the National Southeast Mountain Crop Germplasm Resource Center (Yichun, Jiangxi Province), with climatic conditions of 20-25℃ and 65%-75% humidity. The test material consisted of two-year-old grafted Shine Muscat grape seedlings (Beta rootstock), spaced 4 × 4 m apart, planted on a tall, small V-shaped trellis. The seedlings were virus-free, exhibiting healthy growth, uniform vigor, and consistent flower spike size and development. From each row of Shine Muscat grapes in the same plot, three plants with uniform vigor were selected as experimental materials. From these, 39 fruit spikes were tagged and the following experiments were conducted.

[0024] Twelve fruit clusters were sprayed with water during the full bloom stage (May 3, 2023), and then immersed in different solutions during the young fruit stage (May 12, 2023), as shown in Table 1. One fruit cluster was sprayed with water during the full bloom stage (May 3, 2023) and immersed in water during the young fruit stage (May 12, 2023) as a control.

[0025] Table 1 Experimental conditions for Group A Twelve ear of fruit were sprayed with 25 mg / L GA3 at full bloom (May 3, 2023), and then soaked in different solutions at the young fruit stage (May 12, 2023), as shown in Table 2. One ear of fruit was sprayed with water at full bloom (May 3, 2023) and then soaked in water at the young fruit stage (May 12, 2023) as a control.

[0026] Table 2 Experimental conditions for Group B Twelve ear of fruit were sprayed with 25 mg / L GA3 + 3 mg / L CPPU at full bloom (May 3, 2023), and then soaked in different solutions at the young fruit stage (May 12, 2023), as shown in Table 3. One ear of fruit was sprayed with water at full bloom (May 3, 2023) and then soaked in water at the young fruit stage (May 12, 2023) as a control.

[0027] Table 3 Experimental conditions for Group C After processing, the fruit bunches were collected at the harvest time and relevant physiological indicators were measured, including the weight of the fruit bunch, the weight of the fruit, the longitudinal and transverse diameters of the fruit, the soluble solids content, the titratable acid, and the solid-acid ratio.

[0028] Harvest weighing: The harvested ears were weighed on a commercial platform scale, with 3 biological replicates.

[0029] Fruit weighing: Randomly select 10 fruits from 3 bunches of fruit in the same group and weigh them using an electronic balance scale (purchased from Songjing flagship store).

[0030] Measurement of transverse and longitudinal diameters of fruit berries: After weighing the fruit berries, the transverse and longitudinal diameters of the fruit berries were measured using vernier calipers (purchased from Yongliang Hardware Tools Store); where, the transverse-to-longitudinal ratio = transverse diameter / longitudinal diameter.

[0031] Determination of soluble solids and titratable acid in fruit: Three fruits from the same group were randomly selected and their soluble solids and titratable acid contents were determined using an ATAGO PAL-BX|ACID F5 digital display sugar-acidity meter (purchased from Jiangxi Yunshanggou Supply Chain Co., Ltd.); wherein, the solids-acid ratio = soluble solids / titratable acid.

[0032] Table 4 shows the effects of different treatments on Shine Muscat grape bunches. It can be seen that the overall bunch weight of group A was higher than that of groups B and C. Among them, group A2, with 25 mg / L GA3 + 100 mg / L ALA, had the highest bunch weight at 0.55 kg / bunch, which was superior to group A3 (a combination of 25 mg / L GA3 + 3 mg / L CPPU + 100 mg / L ALA) and group A4 (the traditional combination of 25 mg / L GA3 + 3 mg / L CPPU). This indicates that ALA also has the effect of increasing berry size and retaining fruit, and that the effect of soaking bunches with 25 mg / L GA3 + 100 mg / L ALA is better than that of soaking bunches with 25 mg / L GA3 + 3 mg / L CPPU; furthermore, the combined use of CPPU and ALA actually reduces the effect of ALA.

[0033] Table 4. Analysis of the average weight of grape bunches under different treatments Note: Different lowercase letters in the same column indicate significant differences. p <0.05 Table 5 shows the effects of different treatments on Shine Muscat grape berries. It can be seen that the average berry weight in group A is greater than that in groups B and C, but the differences between the groups are not significant. Groups A2 and B3 have the largest berry weights, at 7.91 g / berry and 7.94 g / berry, respectively. This may be related to the smaller bunch weight in group B3. With sufficient nutrient supply, smaller bunches mean fewer berries, resulting in more nutrients distributed among each berry, leading to fuller berry expansion and increased berry weight.

[0034] Table 5. Analysis of average grape berry weight under different treatments Note: Different lowercase letters in the same column indicate significant differences. p <0.05 The effects of different treatments on the soluble solids content of Sunshine Rose grapes are shown in Table 6. It can be seen that in terms of increasing soluble solids content, group B is significantly better than other groups. Except for groups B3, C3, and C4, which are significantly lower than other groups, there are no significant differences among the other groups. Among them, groups B2 and C2 have the highest soluble solids content, at 19.63% and 19.70%, respectively. Both of them were treated with 25 mg / L GA3 + 100 mg / L ALA soaking in the bunches during the young fruit stage. This indicates that ALA also has a positive effect on increasing the soluble solids content of the fruit.

[0035] Table 6. Analysis of soluble solids content in grapes under different treatments. Note: Different lowercase letters in the same column indicate significant differences. p <0.05.

[0036] The effects of different treatments on the titratable acid content of grapes are shown in Table 7. It can be seen that the titratable acid content in group A is lower than that in groups B and C. Among the subgroups in group A, subgroup A4 has a significantly higher titratable acid content than subgroups A1, A2, and A3, indicating that ALA has a certain acid-reducing function, resulting in a lower titratable acid content than subgroup A4 without ALA. Among the subgroups containing ALA, subgroup A2 (25 mg / L GA3 + 100 mg / L ALA soaking) has the lowest titratable acid content.

[0037] Table 7. Analysis of titratable acid content in grapes under different treatments. Note: Different lowercase letters in the same column indicate significant differences. p <0.05 In horticulture, as fruits ripen, the content of soluble solids increases while the acid content decreases. Therefore, the soluble solids-to-acid ratio is commonly used to evaluate fruit flavor and ripeness. In strawberries, a higher soluble solids-to-acid ratio results in better flavor. Thus, the soluble solids-to-acid ratio is an important quality indicator for evaluating fruit quality. In this invention, the effects of different treatments on the soluble solids-to-acid ratio of grapes are shown in Table 8. It can be seen that groups A2 and B4 have the highest soluble solids-to-acid ratios, at 24.92 and 24.99 respectively. Considering the comprehensive analysis of bunch and berry traits, group A2 shows the best effect, not only increasing bunch and berry weight but also increasing the soluble solids-to-acid ratio and improving fruit flavor.

[0038] Table 8. Analysis of glutamate ratio in different treatments Note: Different lowercase letters in the same column indicate significant differences. p <0.05.

[0039] In summary, ALA is an endogenous plant amino acid that, under low concentration conditions, exhibits dual characteristics similar to auxin and cytokinin. Based on this important theoretical foundation, this invention applies it to the treatment of Shine Muscat grape spikes. During the full bloom stage, the spikes are sprayed with water, and during the young fruit stage, they are soaked in 25 mg / L GA3 + 100 mg / L ALA. This can promote cell division to increase fruit size, increase the soluble solids content of the fruit, reduce the titratable acid content, improve the acid-to-solids ratio, and improve fruit quality. This has important theoretical significance in improving the quality and efficiency of grape production.

[0040] The above description is merely a preferred embodiment of the present invention. The present invention is not limited to the above-described embodiments. Any embodiment that achieves the technical effects of the present invention by the same or equivalent means should fall within the protection scope of the present invention. Within the protection scope of the present invention, various modifications and variations can be made to the technical solutions and / or implementation methods.

Claims

1. A method for cultivating Shine Muscat roses, characterized in that, Includes the following steps: During the peak blooming season of Shine Muscat, apply clean water to the fruit clusters of Shine Muscat. During the young fruit stage of Shine Muscat, the pharmaceutical preparations used for Shine Muscat are applied to the fruit clusters of Shine Muscat. The pharmaceutical preparation for Sunshine Rose includes 5-aminolevulinic acid, gibberellin A3, and a diluent, wherein the concentration of 5-aminolevulinic acid is 80-100 mg / L, and the concentration of gibberellin A3 is 20-30 mg / L.

2. The method according to claim 1, characterized in that, The concentration of 5-aminolevulinic acid is 100 mg / L, and the concentration of gibberellin A3 is 25 mg / L.

3. The method according to claim 1, characterized in that, The diluent is water.

4. The method according to claim 1, characterized in that, Applying water to the fruit clusters of Shine Muscat grapes is called spraying or dipping the clusters; applying the pharmaceutical preparation for Shine Muscat grapes to the fruit clusters is also called spraying or dipping the clusters.

5. The method according to claim 4, characterized in that, Applying water to the fruit clusters of Shine Muscat grapes is called spraying the clusters, while applying the pharmaceutical preparation for Shine Muscat grapes to the fruit clusters is called dipping the clusters.

6. The method according to claim 1, characterized in that, The peak blooming period refers to May 1st to May 7th.

7. The method according to claim 1, characterized in that, The young fruit stage refers to the period from May 7th to May 20th.

8. The method according to claim 1, characterized in that, Apply water to the fruit clusters of Shine Muscat grapes before 10:00 AM or after 4:00 PM.

9. The method according to claim 1, characterized in that, The medication for Sunshine Rose should be applied to the Sunshine Rose before 10:00 AM or after 4:00 PM.

Images