A pesticide composition for preventing and treating walnut weevils

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a kind of pesticide composition for preventing and treating walnut borer.A kind of pesticide composition for preventing and treating walnut borer, which is compounded by first active ingredient and second active ingredient; wherein the first active ingredient is isoxazoline amide, and the second active ingredient is bispropanecyclic insecticide, fluazuron or furan insecticide.The present application compounds isoxazoline amide with existing pesticides, and selects the compounded formula which has synergistic effect on peach borer among them, compared with single pesticide, can improve the prevention and treatment effect on peach borer, and can delay the generation and development of peach borer resistance, and can provide support for preparing the pesticide for preventing and treating walnut borer.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a pesticide composition for controlling the walnut fruit borer. Background Technology

[0002] The peach fruit borer (Conogethespunctiferalis) is an omnivorous pest belonging to the order Lepidoptera, superfamily Pyraloidea, family Pyralidae, subfamily Conogethae, and genus Conogethes. Because its larvae bore into the fruit of various fruit trees, it is also known as the heart-eating moth, heart-boring moth, and heart-drilling moth. It has over 100 host plant species, damaging peach, chestnut, pear, walnut, pomegranate, loquat, longan, and fig trees, as well as crops such as corn and sorghum. It can migrate between different hosts, severely impacting fruit quality and the yield and quality of grains and oils. While pesticides can be used for control during production, the peach fruit borer has developed varying degrees of resistance with long-term use of existing pesticides, leading to reduced control effectiveness. Therefore, developing new pesticides for controlling the peach fruit borer is essential.

[0003] Isoxazolamide, developed by Syngenta, is an isoxazoline insecticide and an allosteric modulator of the γ-aminobutyric acid-gated chloride channel (GABA-Cl) receptor. It exerts its insecticidal effect by selectively blocking inhibitory neurotransmission in invertebrates via the anti-dilhardt GABA-Cl receptor subunit (RDL), exhibiting excellent safety in mammals. Isoxazolamide demonstrates high insecticidal activity against a wide range of pests, including Lepidoptera, Hemiptera, Thysanoptera, and Acari, and has broad market development and application prospects in crop protection.

[0004] While isoxaflutole has the advantages mentioned above, as a pesticide, long-term use of a single component will inevitably lead to pesticide resistance in pests, resulting in reduced control efficacy. However, rationally combining different pesticides and selecting formulations with synergistic effects can improve control of target pests while reducing the risk of resistance, which undoubtedly provides some protection for isoxaflutole.

[0005] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0006] The purpose of this invention is to provide a pesticide composition for controlling the peach borer, which exhibits a synergistic effect in killing the peach borer and can improve the control efficacy against it. This can provide support for the development of pesticides for controlling the peach borer.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] A pesticide composition for controlling the walnut fruit borer, comprising a first active ingredient and a second active ingredient; wherein the first active ingredient is isoxazoline, and the second active ingredient is bispyribac-methyl, flonicamid, or furazolidone.

[0009] Preferably, when the second active ingredient is bispyribac-methyl, the mass ratio of the first active ingredient to the second active ingredient is 1-30:30-1.

[0010] Preferably, when the second active ingredient is flonicamid, the mass ratio of the first active ingredient to the second active ingredient is 1-10:2-1.

[0011] Preferably, when the second active ingredient is furazolidone, the mass ratio of the first active ingredient to the second active ingredient is 1-8:40-1.

[0012] The present invention also provides the use of the pesticide composition in the preparation of a pesticide for controlling the walnut fruit borer.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] (1) This invention combines isoxazoline with existing agents and selects the compound formulation that has a synergistic effect on peach borer. Compared with single agents, it can improve the control effect on peach borer. Based on this, the amount of pesticide used can be reduced, the control cost and pesticide residue can be reduced.

[0015] (2) The pesticide composition of the present invention for controlling the walnut fruit borer can reduce the risk of pesticide resistance, delay the emergence and development of pesticide resistance in the walnut fruit borer, and provide support for the preparation of pesticides for controlling the walnut fruit borer.

[0016] (3) The pesticide composition of the present invention for controlling walnut fruit borer can provide a certain degree of protection against isoxazoline. Detailed Implementation

[0017] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0018] Combined toxicity testing trial

[0019] 1. Test pests

[0020] The peach borer was collected from walnut-growing areas. After being reared with chestnuts for 8 generations in an artificial climate chamber with a temperature of (25±1)℃, relative humidity of (70±5)%, and photoperiod of 15h:9h (L:D), healthy 3rd instar larvae with basically the same body size were selected as the test pests.

[0021] 2. Test reagents

[0022] 96% isoxaflutole technical grade, 92.5% bifenthrin technical grade, 95.9% flonicamid technical grade, 98% furazolidone technical grade.

[0023] After completely dissolving the original drug in dimethyl sulfoxide, it was diluted with 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution of 10,000 mg / L, which was then stored at 4°C for later use.

[0024] 3. Test methods

[0025] The toxicity of isoxaflutole, bifenthrin, flonicamid, furazolidone, and their mixtures to peach fruit borer was determined by the feed immersion method.

[0026] 3.1 Determination of the toxicity of a single agent against the peach fruit borer

[0027] The test reagent was diluted with a 0.1% (v / v) Tween-80 aqueous solution to create five mass concentration gradients. Chestnuts were cut into pieces with an area of ​​1 cm². 2 Thin slices, 2 mm thick, were soaked in the pesticide solution for 20 seconds, removed, air-dried, and placed in a 9 cm diameter petri dish, along with 15 test pests. Each pesticide concentration was tested in triplicate, with a 0.1% (v / v) Tween-80 aqueous solution serving as a blank control. The treated pests were then placed in an artificial climate chamber with a temperature of (25±1)℃, relative humidity of (70±5)%, and a photoperiod of 15 h:9 h (L:D). After 72 h, the mortality rate of the pests was recorded; pests that did not move when lightly touched with a brush were considered dead. The corrected mortality rate for each treatment was calculated. Linear regression analysis was performed on the corrected mortality rate probability values ​​of the pests and the logarithmic values ​​of the pesticide concentrations to derive the toxicity regression equation and LC-1 for each single agent against the peach fruit borer. 50 value.

[0028]

[0029]

[0030] 3.2 Determination of the toxicity of different formulations to peach fruit borer

[0031] Based on the single-agent toxicity test results, multiple formulations of isoxaflutole were prepared with bispyribac-methyl, flonicamid, or furazolidone. Each formulation was diluted with a 0.1% (v / v) Tween-80 aqueous solution to create five mass concentration gradients. The formulations were prepared and used immediately. The corrected mortality rate of each formulation against the tested pests was determined according to the toxicity assay method in section 3.1 (with the 0.1% (v / v) Tween-80 aqueous solution treatment serving as a blank control). Regression analysis was performed on the corrected mortality rate probability values ​​of the tested pests and the logarithmic values ​​of the pesticide concentrations to determine the LC50 of each formulation against the tested pests. 50 value.

[0032] 4. Data Analysis

[0033] The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method. Based on the criteria for classifying combined effects: a CTC value ≥ 120 indicates a synergistic effect; a CTC value ≤ 80 indicates an antagonistic effect; and a CTC value < 80 < 120 indicates an additive effect. The results are shown in Tables 1-4.

[0034] Table 1. Results of toxicity tests of each single agent against peach fruit borer.

[0035]

[0036] Table 1 shows the LC50 toxicity of isoxaflutole, diproteil, flonicamid, and furazolidone against peach borer. 50 The concentrations were 1.074 mg / L, 3.3169 mg / L, 8.5284 mg / L, and 11.3792 mg / L, respectively.

[0037] Table 2. Results of toxicity tests on peach fruit borer using different ratios of isoxazoline and diclofenac mixtures.

[0038]

[0039] As shown in Table 2, the co-toxicity coefficients of isoxaflutole and bispyribac-methyl in the peach borer at a mass ratio of 1-30:30-1 are all greater than 120, indicating that the combination of isoxaflutole and bispyribac-methyl has a synergistic effect on killing the peach borer.

[0040] Table 3. Results of toxicity tests on peach fruit borer using different ratios of isoxaflutole and flonicamid mixtures.

[0041]

[0042] As shown in Table 3, the co-toxicity coefficients of isoxaflutole and flonicamid against peach borer are all greater than 120 when the mass ratio is 1-10:2-1, indicating that the combination of isoxaflutole and flonicamid has a synergistic effect on killing peach borer.

[0043] Table 4. Results of toxicity tests on peach borer using different ratios of isoxazoline and furazolidone mixtures.

[0044]

[0045] As shown in Table 4, the co-toxicity coefficients of isoxazoline and furazolidone against peach borer are all greater than 120 when the mass ratio is 1-8:40-1, indicating that the combination of isoxazoline and furazolidone has a synergistic effect on killing peach borer.

[0046] In conclusion, when isoxazoline is combined with bispyribac-methyl, flonicamid, or furazolidone in a certain mass ratio, it exhibits a synergistic effect in killing peach borers, improving the control efficacy against peach borers and providing support for the development of pesticides to control peach borers.

[0047] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A pesticide composition, characterized in that, It is composed of a first active ingredient and a second active ingredient; wherein the first active ingredient is isoxazoline, and the second active ingredient is bispyribac-methyl; the mass ratio of the first active ingredient to the second active ingredient is 1-30:30-1.

2. The use of the pesticide composition according to claim 1 in the preparation of an agent for controlling walnut fruit borer.

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