Bacteria-based mortise and tenon assembly type gradient water purification and storage roadbed and construction method thereof

Abstract

The application discloses a kind of gradient water purification water storage subgrade based on bacteria and its construction method, and its roadbed structure includes construction waste recycled aggregate roadbed, seepage ditch, anti-seepage interlayer, drain pipe, slope fill, anchor rod;The material used in the application is recycled aggregate after construction waste treatment, which can effectively reduce the landfill amount of construction waste, alleviate the solid waste disposal problem, reduce the waste of land resources and environmental pollution;Through braided belt, gabion can realize the rapid construction of roadbed assembly, and is suitable for emergency access protection engineering urgently needed in ecological sensitive area disaster rescue;Prefabricated gabion sets mortise and tenon structure and is assembled by mortise and tenon+insertion mode, which can effectively improve the initial bearing capacity of roadbed;The application repairs the crack of recycled aggregate by microbial mineralization and other methods, strengthens the structure itself, further improves the mechanical properties and durability with time, thereby improves the strength and improves the stability of the whole roadbed.

Description

Technical Field

[0001] This invention belongs to the field of environmentally friendly building technology, and in particular relates to a bacteria-based mortise and tenon assembled gradient water purification and storage roadbed and its construction method. Background Technology

[0002] The rise in economic levels and urbanization has brought about a surge in construction activities, such as building, demolition, renovation, and expansion, generating substantial amounts of construction waste. Vehicle traffic, road de-icing, and dust pollution cause the accumulation of various environmental pollutants on roads, including particulate matter, nitrogen oxides, volatile organic compounds, inorganic salts, and heavy metals, turning roads into pollution sources. These pollutants are highly mobile and, under rainfall conditions, can enter road structures and seep into groundwater, causing health problems for the public. To address the increasingly serious environmental issues and meet the demand for green building materials, using recycled aggregates obtained from crushing and processing construction waste in engineering projects has become a solution to the construction waste management problem. In road engineering, the main method of utilizing recycled aggregates is in roadbed filling. However, due to the presence of micro-cracks and pores within recycled aggregates, their physical and mechanical properties are inferior to natural aggregates, specifically exhibiting lower density, higher crushing value, and higher water absorption, significantly affecting the roadbed's bearing capacity.

[0003] Based on this, patent CN116425440A discloses a type of microbial concrete reinforcing particle and its application, which uses microorganisms including Bacillus pasteurellii to modify zeolite particles to obtain concrete reinforcing particles with better compressive strength; however, the nutrients and calcium sources obtained by the microorganisms used in the modification process can only be obtained from additives, making the entire process unsustainable; other existing technologies mostly use gabion mesh and other means to simply fix large aggregates of construction waste in road construction, but recycled aggregates of construction waste generally have a series of problems such as low density, high water absorption, and high crushing value. The surface of recycled aggregates has old cement mortar and cracks formed due to long-term use and mechanical crushing, and there are a large number of pores inside (such as... Figure 1 This weakens the mechanical properties and durability of recycled aggregates. The water absorption rate of recycled aggregates is significantly increased due to the adhesion of cement mortar to the surface and the cracks and pores created internally, resulting in a marked decrease in mechanical properties. While existing modification technologies for recycled construction waste aggregates can improve their performance, they also have some potential drawbacks and limitations. Many modification technologies for recycled construction waste aggregates require additional equipment, materials, and processes, increasing processing costs. Modifiers may lose stability under long-term use or extreme environmental conditions, thus reducing the modification effect. Furthermore, some modification methods may have negative environmental impacts, such as releasing harmful substances or polluting soil and groundwater.

[0004] Given that recycled aggregates from construction waste have numerous cracks and their mechanical properties deteriorate significantly after absorbing water, the long-term road performance of roadbeds filled with construction waste, especially their long-term cumulative deformation characteristics under immersion conditions, needs further improvement. Many modification technologies for recycled aggregates from construction waste require additional equipment, materials, and processes, increasing processing costs. Some modifiers may lose stability under long-term use or extreme environmental conditions, thereby reducing the modification effect. Meanwhile, some modification methods may have negative environmental impacts, such as releasing harmful substances or polluting soil and groundwater; existing technologies for modifying roadbed materials using microorganisms are difficult to achieve long-term bacterial growth; furthermore, existing roadbed construction methods are difficult to implement for rapid repair after damage caused by natural disasters or other reasons, especially in ecologically sensitive areas where roadbeds collapse due to rain and flooding. On the one hand, the strength of the road surface and roadbed leads to irregular and large-area erosion areas; on the other hand, the construction methods increase the difficulty of repair. In addition, traditional construction methods may cause a large amount of pollution in ecologically sensitive areas, which may further damage the ecology. Therefore, researching a convenient, rapid, environmentally friendly, and sustainable roadbed material and construction method made from recycled construction waste has significant engineering value and ecological benefits. Summary of the Invention

[0005] The purpose of this invention is to provide a bacteria-based mortise and tenon assembled gradient water purification and storage roadbed, which uses recycled aggregate from construction waste to fill the water storage roadbed and uses microbial mineralization technology to strengthen its strength. Through a series of actions such as physical adsorption, biological decomposition, and biological solidification, pollutants are purified in a gradient manner to solve the environmental pollution problem of roads, and realize a water storage roadbed structure that is simple to assemble, environmentally friendly, sustainable, and has an integrated flexible high-strength structure.

[0006] Another objective of this invention is to provide a bacterial-based mortise and tenon joint assembly gradient water purification and storage roadbed construction method.

[0007] To address the aforementioned issues, a bacterial-based mortise and tenon joint assembly-type gradient water purification and storage roadbed construction method is proposed, comprising the following steps:

[0008] S1. Screening construction waste to obtain recycled aggregate;

[0009] S2. Place the woven bag into the prefabricated gabion, put the screened recycled aggregate mixed with sludge into the woven bag, and place the gabion together into the metal mold.

[0010] S3. Install the prefabricated gabions to complete the main roadbed construction;

[0011] S4. Construct seepage-proof interlayers on both sides of the roadbed;

[0012] S5. Cover the surface of the seepage-proof interlayer with a layer of geotextile, and then fill with soil to form a slope backfill.

[0013] S6. Install drainage switches on water supply and drainage pipes; inject nutrients and bacteria into the roadbed.

[0014] Furthermore, in step S1, recycled aggregate with a particle size range of 5-25mm is selected by screening; construction waste with excessively large particle sizes is crushed and then screened again; the prefabricated gabion is made of low-carbon steel wire with a metal mesh on the surface, and the mesh is a multi-stranded hexagonal shape; the woven bag is made of PET polyester woven bag.

[0015] Furthermore, the prefabricated gabion has pre-drilled holes on its top surface and steel bar pins on its bottom surface; each side is provided with a mortise and tenon structure, the gabion includes gabions with mortises on the sides and gabions with tenons on the sides, the mortises are square grooves with a bottom larger than the opening groove, the bottom rectangular grooves are larger than the opening groove, the tenons are square bosses, the outer bottom surface of the boss is larger than the cross section at the connection between the boss and the gabion.

[0016] Furthermore, in S2, the sludge accounts for no more than 30% of the volume of the recycled aggregate.

[0017] Furthermore, the specific steps of S3 are as follows:

[0018] Prefabricated gabions are installed layer by layer in the roadbed pit, from bottom to top. Gabions with mortises on the sides and gabions with tenons on the sides are installed alternately. Each tenon is inserted into the mortise. The side of the gabion with the steel pin faces upward and the side with the insertion hole faces downward. The steel pin at the top of the lower gabion is inserted into the insertion hole at the bottom of the upper gabion. The gaps in the gabions are filled with sieved fragments, mineral powder, and silt; the silt accounts for no more than 30% of the volume of the fragments and mineral powder. Each layer of gabions... A 0.45–0.55 cm thick geogrid is laid between the gabions. After each layer of gabions and geogrid is laid, a PVC capillary drainage strip is laid on top of it. Then, an adsorption layer of 4–6 cm thick composed of construction waste and expanded clay is laid on top of the drainage strip. Drainage pipes are installed at the adsorption layer locations on both sides of the road, with a slope of 4%–5%. Anchor bolts are reserved between each layer of gabions. The spacing between drainage pipes is 0.5–1.2 m, and the spacing between anchor bolts is 0.5–1.8 m.

[0019] Furthermore, if the road surface is impermeable, a seepage ditch with a width of 0.7 to 0.9 m and a depth of 0.4 to 0.5 m should be reserved on the uppermost layer of the roadbed.

[0020] Furthermore, the seepage-proof interlayer in S4 is a reinforced concrete panel with a thickness of 6-10cm, which is anchored to the gabion inside the roadbed and between the soil on both sides using anchor rods; the seepage-proof interlayers on both sides of the roadbed share a common anchor rod.

[0021] Furthermore, in S5, the slope of the slope fill is 1:1 to 1:1.5; the slope surface is mainly planted with herbaceous or vine plants; and shrubs and trees are planted as a supplement.

[0022] Furthermore, S6 injects nutrients into the roadbed, the nutrients including one or more of water, carbon source, energy source, nitrogen source, inorganic salts and growth factors, the bacteria including one or more of Escherichia coli, Staphylococcus, Pasteurella multocida, Pasteurella multocida, and Bacillus megaterium, and the energy source including glucose or agar.

[0023] A bacteria-based mortise and tenon joint assembled gradient water purification and storage roadbed was constructed according to the above construction method.

[0024] The beneficial effects of this invention are as follows: The method of this invention processes construction waste into recycled aggregate for roadbed construction, effectively reducing the amount of construction waste landfilling, alleviating the problem of solid waste disposal, and reducing the waste of land resources and environmental pollution. Furthermore, recycled aggregate can meet people's demand for low-carbon production and green building materials, aligning with the goals of sustainable development. Prefabricated roadbed construction using woven belts and gabions enables rapid prefabricated construction, effectively solving the problem of rapid roadbed construction for emergency road maintenance in ecologically sensitive areas after disasters. The prefabricated gabions, with their mortise and tenon structure and assembled using a combination of mortise and tenon joints and interlocking, effectively improve the initial bearing capacity of the roadbed. This method can repair cracks in the recycled aggregate through microbial mineralization, self-strengthening its structure and further improving its mechanical properties and durability over time, thereby enhancing its strength and the overall stability of the roadbed. In addition, the adsorption layer composed of construction waste and ceramsite has a strong adsorption capacity, further intercepting and adsorbing residual pollutants. The combination of these structures ultimately forms a gradient self-purification function for the water-storage roadbed. When the roadbed's adsorption capacity reaches its limit or becomes clogged, pollutants and silt can be removed from the roadbed through roadbed cleaning, thus achieving the sustainability of the roadbed's self-cleaning function. This invention provides a precast gabion and a mortise and tenon + interlocking gabion assembly method. The splicing of adjacent gabions increases the overall integrity between precast gabions in the same layer; it improves pavement stability and load-bearing capacity, reduces lateral deformation and vertical settlement of the roadbed, and extends its service life; it also reduces roadbed deformation and cracking caused by uneven settlement of the underlying subgrade, and enables rapid repair of damaged pavements. Furthermore, splicing between upper and lower layers increases the connectivity between layers, enhancing the overall stability of the roadbed, improving the pavement's compressive and flexural strength, reducing cracks and potholes, and improving pavement smoothness and comfort. Simultaneously, the increased vertical strength reduces pavement rebound and vibration, lowering vehicle fuel consumption and noise pollution; it also withstands impacts from water flow, debris flows, earthquakes, and other loads on the roadbed, reducing roadbed deformation. This invention is of great significance in reducing the amount of construction waste going to landfills, protecting the environment, and conserving resources, and provides an environmentally friendly and economical solution for sustainable development. Attached Figure Description

[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0026] Figure 1 This is a SEM image of the surface of recycled aggregate from construction waste;

[0027] Figure 2 This is a diagram of a construction waste water storage roadbed structure;

[0028] Figure 3 It is a component of construction waste gabions, of which (a) is recycled aggregate, (b) is PET polyester woven bags, and (c) is gabions;

[0029] Figure 4 This is a top view of the gabion assembly method according to an embodiment of the present invention;

[0030] Figure 5 This is a schematic diagram of a roadbed made of recycled aggregate from construction waste suitable for impermeable pavement in Embodiment 1 of the present invention;

[0031] Figure 6 This is a schematic diagram of a construction waste recycled aggregate roadbed suitable for permeable pavement according to Embodiment 2 of the present invention;

[0032] Figure 7 This is a schematic diagram of the simulation system for constructing recycled aggregates in Embodiment 3 of the present invention;

[0033] Figure 8 This is a side view of the mortise and tenon assembly method of the gabion according to an embodiment of the present invention.

[0034] The components include: 1. Main roadbed; 2. Seepage ditch; 3. Anti-seepage interlayer; 4. Drainage pipe; 5. Slope backfill; 6. Anchor bolts. Detailed Implementation

[0035] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0036] This invention provides, for example Figure 2 A type of bacteria-based mortise and tenon joint assembled gradient water purification and storage roadbed, the specific construction steps of which are as follows:

[0037] S1. Screen the construction waste to select particles with a diameter range of 5–25 mm for use as raw materials for prefabricated gabions. Construction waste with excessively large particles can be crushed and screened again for reuse.

[0038] S2. Place PET polyester woven bags into prefabricated gabions. The gabions are made of low-carbon steel wire with a metal mesh on the surface; the mesh is a multi-stranded hexagonal pattern. Mix construction waste with sludge, not exceeding 30% of the construction waste volume, and place it into the geotextile woven bags, then compact it. To prevent the geotextile woven bags from breaking, place the woven bags and gabions in a metal mold. Each geotextile bag and gabion is a rectangular prism.

[0039] S3. The prefabricated gabions are installed layer by layer in the roadbed pit from bottom to top. After each layer is installed, the gabions are connected using steel bars or anchor bolts 6. The gabion shape of this invention is as follows: Figure 4 , Figure 8 The gabion has pre-drilled holes on its top surface and steel bar pins on its bottom surface. Each side features a mortise and tenon structure. The gabion includes gabions with mortises on their sides and gabions with tenons on their sides. The mortises are square grooves with a bottom larger than the opening, and rectangular grooves with a bottom larger than the opening. The tenons are square bosses with an outer bottom surface larger than the cross-section at the connection point between the boss and the gabion, ensuring the tenon fits snugly into the mortise. Specific connection methods are as follows: Figure 8 The gabions are assembled using horizontal tenon and mortise joints and vertical pins. The gaps in the gabions are filled with fragments, mineral powder, and silt from the screening of construction waste, with the silt accounting for no more than 30% of the volume of the construction waste fragments and mineral powder. Before laying the next layer of gabions, a geogrid approximately 0.45–0.55 cm thick is laid. After each layer of gabions and geogrid is laid, a layer of PVC capillary drainage tape is laid on top, followed by an absorbent layer of construction waste and expanded clay aggregate with a thickness of 4–6 cm above the drainage tape. Anchor bolts 6 are pre-installed between each layer of gabions. Drainage pipes 4 are installed at the absorbent layer locations on both sides of the road, with a slope of 4%–5% to facilitate drainage. The spacing of the drainage pipes 4 is approximately 0.5–1.2 m, and the length depends on the distance from the roadbed slope. The drainage pipes 4 not only serve a drainage function but also allow for the injection of nutrients. The PVC capillary drainage tape combines the functions of rapid drainage and rapid nutrient transport. The adsorption layer composed of construction waste and expanded clay aggregate can effectively adsorb various organic or inorganic pollutants, achieving deep water purification. When the roadbed becomes clogged or needs cleaning, clean water or a solution containing pollutant removal agents can be injected through a seepage ditch or water pipe on one side of the roadbed, while a pump creates negative pressure on the other side to clean the roadbed. At this time, PVC capillary drainage strips can reduce fluid infiltration to improve cleaning efficiency. The spacing of anchor bolts 6 is 0.5-1.8m, and the specifications selected according to GB / T50119-2007 "Technical Specification for Rock and Soil Anchor Support" are determined based on the roadbed height. When the pavement is impermeable, a seepage ditch 2 with a width of 0.7-0.9m and a depth of approximately 0.4-0.5m is reserved at the top layer of the roadbed. Its function is to collect rainwater from the pavement and drain it into the roadbed.

[0040] The mortise and tenon structure used in this invention is mainly straight tenon or dovetail tenon, which is relatively simple in structure. Its main purpose is to increase the integrity between different modules, improve its strength and stability, and prevent it from easily loosening under dynamic loads such as earthquakes and traffic loads. Although prefabricated gabions themselves have a certain structural integrity, their structural integrity is limited by the internal filling material and reinforcing steel, and cannot be too complex. In addition, since the compressive strength of the filling material inside the prefabricated gabion is much greater than its tensile strength, if the shape is too complex, it may lead to excessive tensile stress and failure of the structure. Therefore, it is necessary to choose a simple structural component form with reasonable stress distribution.

[0041] The mortise and tenon joint + interlocking form of the gabion described in the accompanying drawings and embodiments of this invention is only one example. The specific mortise and tenon joint and interlocking method can be other mortise and tenon joint and interlocking methods according to actual needs, or it can be a combination of multiple mortise and tenon structures to meet the specific structure of different terrains and working conditions.

[0042] This invention utilizes mortise and tenon joints and interlocking to assemble precast gabions. The splicing of adjacent gabions increases the overall integrity of the same layer, improving road stability and load-bearing capacity, reducing lateral deformation and vertical settlement of the roadbed, and extending its service life. It also reduces roadbed deformation and cracking caused by uneven settlement of the underlying subgrade. The splicing between upper and lower layers increases interlayer connectivity, enhancing the overall stability of the roadbed, improving the road surface's compressive and flexural strength, reducing cracks and potholes, and improving road smoothness and comfort. Simultaneously, the increased vertical strength reduces road rebound and vibration, lowering fuel consumption and noise pollution. Furthermore, the interlocking gabions can withstand impacts from water flow, debris flows, earthquakes, and other loads on the roadbed, reducing deformation.

[0043] S4. After the main roadbed 1 is filled, the seepage-proof interlayer 3 is laid on both sides of the roadbed. The seepage-proof interlayer 3 is a 6-10cm thick reinforced concrete panel, which is anchored to the gabion inside the roadbed and between the slope soil on both sides using anchor bolts 6. The seepage-proof interlayer 3 on both sides of the roadbed shares a common anchor bolt 6 to improve the overall integrity of the roadbed.

[0044] S5. Cover the surface of the seepage-proof interlayer 3 with a layer of geotextile to provide waterproofing, and then fill with soil to form the slope fill 5. The slope of the slope fill 5 is 1:1 to 1:1.5. The slope surface should be mainly planted with herbaceous or vine plants, supplemented by a small number of shrubs and trees. The root system of the plants will help prevent soil erosion and also reinforce the slope soil to increase its strength.

[0045] S6. A switch is installed on the drainage pipe 4 to regulate the water level within the roadbed. Bacteria and nutrients, including water, carbon sources (such as sugars), energy (such as glucose and agar), nitrogen sources (such as proteins, urea, and ammonium salts), inorganic salts, and some growth factors, are injected into the roadbed through the drainage pipe 4. A single substance may simultaneously provide multiple nutrients. [The text abruptly ends here, likely due to an incomplete sentence or missing information.] Figure 3 (a) Recycled aggregate is placed in (b) PET polyester woven bags, which are then placed in (c) metal gabions to form a structure like... Figure 4 Precast gabions for construction waste. Building materials can be prepared in advance, enabling prefabricated construction.

[0046] In this embodiment, bacteria can form a biofilm structure between silt and aggregate; the bacteria undergo a mineralization reaction to generate calcium carbonate precipitate, which binds the recycled aggregate particles. The bacteria must include urease-producing bacteria, such as *Escherichia coli*, *Staphylococcus*, *Pasteurella multocida*, *Pasteurella macrantha*, and *Bacillus megaterium*. The nutrients in this invention are only to promote the rapid growth of bacteria in the roadbed. After the added nutrients are exhausted, subsequent reactions can still be carried out using the influencing substances in the silt and the calcium source in the recycled aggregate, forming a sustainable ecosystem. When polluted water enters the roadbed, construction waste can adsorb various pollutants, while silt has adsorption and blocking effects, increasing the residence time of pollutants in the roadbed and providing sufficient reaction time for the adsorption, decomposition, and solidification of pollutants. Furthermore, the bacteria in the roadbed can absorb and decompose organic pollutants and nitrogen oxides in rainwater. Bacteria with mineralization capabilities (urease-producing bacteria) can further solidify pollutants to form stable carbonates. Furthermore, the adsorption layer composed of construction waste and expanded clay aggregate possesses strong adsorption capacity, further intercepting and adsorbing residual pollutants. This structural combination ultimately forms the gradient self-cleaning function of the water-storage roadbed. When the roadbed's adsorption capacity reaches its limit or siltation occurs, pollutants and silt can be removed from the roadbed through roadbed cleaning, thus ensuring the sustainability of the roadbed's self-cleaning function.

[0047] Example 1

[0048] like Figure 5This embodiment describes a prefabricated gradient water purification and storage roadbed based on bacteria, suitable for impermeable pavements. The roadbed is 4m high and 6m wide. Mineral powder, fine aggregate, and silt obtained after screening construction waste are compacted and placed into PET polyester woven bags, which are then placed inside metal gabions. The roadbed is laid in layers, with each layer of gabions 0.5m high, for a total of 8 layers. The gaps in the gabions are filled with fragments, mineral powder, and silt. Before laying the next layer of gabions, a 0.5cm thick geogrid made of high-density polyethylene is laid. After each layer of gabions and geogrid is laid, a layer of PVC capillary drainage tape is laid on top, followed by a 4cm thick adsorption layer composed of construction waste and expanded clay aggregate. Drainage pipes 4 are installed at the adsorption layer locations on both sides of the road, with a 4% slope for drainage. Anchor bolts 6 are pre-installed in each layer of the roadbed. The drainage pipes 4 are spaced 1.2m apart, and the anchor bolts 6 are spaced 1.5m apart. After the roadbed is paved, a seepage ditch 2 with a width of 0.5m and a depth of 0.5m is reserved on the top layer. An impermeable interlayer 3, consisting of reinforced concrete panels with a thickness of 10cm, is installed on both sides of the roadbed. The two impermeable interlayers 3 are connected by anchor bolts 6 with a length of 6m. A layer of geotextile is covered on the concrete panel surface to create a slope, followed by backfilling with soil at a 1:1 slope. Bacteria and nutrients are injected into the roadbed through drainage pipes 4. The bacteria are a solution of Bacillus pasteurellium (OD200). 600 >1) Inject a 1.0 mol / L calcium acetate solution, with nutrients consisting of 10 g peptone, 6 g yeast extract, and 10 g sodium chloride per liter of water, at a pH of 7.0. After construction, plant suitable slope protection plants in the backfill soil.

[0049] Example 2

[0050] like Figure 6This embodiment describes a prefabricated gradient water purification and storage roadbed based on bacteria, suitable for permeable pavement. The roadbed is 5m high and 8m wide. Since the permeable pavement allows water to drain directly into the top of the roadbed, a separate seepage ditch 2 is not required. Mineral powder, fine aggregate, and silt obtained after screening construction waste are compacted and placed into PET polyester woven bags, which are then placed inside metal gabions. The roadbed is laid in layers, with each layer of gabions 0.5m high, for a total of 10 layers. The pores are filled with mineral powder and silt. Before laying the next layer of gabions, a 1cm thick geogrid made of high-density polyethylene is laid. After each layer of gabions and geogrid is laid, a PVC capillary drainage strip is laid on top, followed by a 4cm thick adsorption layer composed of construction waste and expanded clay aggregate. Drainage pipes 4 are installed at the adsorption layer locations on both sides of the road, with a 4% slope for drainage. Anchor bolts 6 are pre-installed at each roadbed layer. The drainage pipes 4 are spaced 1m horizontally and 0.5m vertically; the anchor bolts 6 are spaced 1.8m horizontally and 0.5m vertically. After the roadbed construction is completed, a reinforced concrete anti-seepage interlayer 3, 8cm thick, is installed on both sides of the roadbed. The two interlayers are connected by anchor bolts 6, each 8m long. A geotextile slope is laid on the concrete panel surface, followed by backfilling at a slope of 1:1.5. After the roadbed and slope backfilling 5 are completed, the pavement is constructed as permeable concrete. Finally, bacteria and nutrients are injected into the roadbed through the drainage pipes 4. The bacteria are Pasteurella multocida solution (OD200). 600 >1) Inject a 0.75 mol / L calcium chloride solution. The nutrient composition is 12 g peptone, 10 g yeast extract, and 12 g sodium chloride per liter of water, with a pH of 7.0. After construction is completed, plant suitable slope protection plants in the backfill soil.

[0051] Example 3

[0052] To demonstrate the feasibility of this invention, a system was constructed using recycled aggregates from construction waste, such as... Figure 7 As shown. A cylindrical container with a diameter of 10cm and a length of 60cm was constructed using acrylic material. Recycled aggregate, obtained from screening construction waste, was layered inside the container, compacted in 3cm increments, and layered to the corresponding height. Bacillus pasteurellis was cultured, and OD... 600 The pH value was 1.0, and the conductivity of the bacterial solution was 0.40 mS / cm. The bacterial solution was injected into the regenerated aggregate until saturation was achieved using a peristaltic pump. Subsequently, 3 L of nutrients were injected using a peristaltic pump. The nutrient composition consisted of 10 g peptone, 6 g yeast extract, and 10 g sodium chloride per liter of water, with a pH of 7.0. The nutrient solution was continuously injected at a rate of 50 mL / h, and the OD of the outflowing liquid was tested periodically through the bottom outlet. 600pH value and electrical conductivity were used to assess the sustainability of the ecosystem. The data in Table 1 show that the ecosystem constructed using the above method still has strong viability after one month.

[0053] Table 1. Various indicators of the outflowing liquid after different time periods.

[0054]

[0055] This invention abandons the traditional approach of using only recycled coarse aggregate in gabion mesh. Instead, it integrates recycled coarse aggregate, fine aggregate, mineral powder, and silt as a whole. The recycled aggregate is fixed in place by woven bags, silt, and gabions to reduce displacement. Different gabions are connected by reinforcing bars or anchors, and different layers of gabions are connected using geogrids. Lateral constraints are applied using cement panels on both sides of the roadbed, thus forming a flexible, prefabricated, integrated load-bearing structure. After combining geogrid bags with gabion mesh, the fine aggregate, mineral powder, and silt are protected from rainwater erosion and effectively fill structural voids, thereby dispersing structural stress and improving structural load-bearing capacity. Furthermore, the fine aggregate, mineral powder, and silt in the water-storage roadbed are constantly moist, and the bacterial film further cements the structure, making it resemble a gelatinous substance. This absorbs and buffers the impact of dynamic loads, transforming the traditional rigid roadbed into a flexible one. Furthermore, the overall integrity of the roadbed and the structural stress can be further improved by using mineralization reactions to bind fine aggregates, mineral powder, and silt; anchoring or reinforcing steel bars to connect gabion meshes; confining the sides of the roadbed with concrete slabs; and applying geogrids between multiple layers of gabion meshes. Mineralization reactions not only continuously strengthen the bonds between recycled aggregates, mineral powder, and silt, but also continuously repair cracks caused by vehicle loads.

[0056] The roadbed described in this invention is itself a water-storage structure, capable of storing rainwater within the roadbed. The water level can be adjusted via drainage pipe 4, and the discharged wastewater can be used to irrigate vegetation on the roadbed slope. Furthermore, the silt contains nutrients, enabling long-term bacterial growth and sustained mineralization reactions, resulting in continuous improvement in structural strength, self-repair of cracks, and long-term purification of pollutants. Compared to traditional permeable roadbeds, the road structure of this invention is clearly more environmentally friendly.

[0057] The various embodiments in this specification are described in a related manner. Similar or identical parts between embodiments can be referred to mutually. Each embodiment focuses on describing the differences from other embodiments. In particular, the system embodiments are basically similar to the method embodiments, so the description is relatively simple; relevant parts can be referred to the descriptions of the method embodiments.

[0058] The above description is merely a preferred embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention are included within the scope of protection of the present invention.

Claims

1. A bacterial-based mortise and tenon assembly type gradient water purification and storage roadbed construction method, characterized in that, Includes the following steps: S1. Screening construction waste to obtain recycled aggregate; S2. Place the woven bag into the prefabricated gabion, put the screened recycled aggregate mixed with sludge into the woven bag, and place the gabion together into the metal mold. S3. Install the prefabricated gabions to complete the construction of the main roadbed (1); the prefabricated gabions have pre-reserved insertion holes on the top surface and steel bar pins on the bottom surface; each side is provided with a mortise and tenon structure, the gabions include gabions with mortises on the sides and gabions with tenons on the sides, the mortises are square grooves with the bottom larger than the opening grooves, the bottom rectangles are larger than the opening grooves, the tenons are square bosses, the outer bottom surface of the bosses is larger than the cross section at the connection between the bosses and the gabions; the specific steps are as follows: Prefabricated gabions are installed layer by layer in the roadbed pit from bottom to top. Gabions with mortises on the sides and gabions with tenons on the sides are installed alternately. Each tenon is inserted into the mortise. The side of the gabion with the steel bar pins faces upward and the side with the insertion hole faces downward. The steel bar pins at the top of the lower gabion are inserted into the insertion holes at the bottom of the upper gabion. The gaps in the gabions are filled with sieved fragments, mineral powder, and silt. The silt accounts for no more than 30% of the volume of the fragments and mineral powder. A 0.45-0.55 cm thick geogrid is laid between each layer of gabions. After each layer of gabions and geogrid is laid, a layer of PVC capillary drainage tape is laid on top, followed by a 4-6 cm thick layer of geogrid. A layer of adsorption material consisting of construction waste and ceramsite is formed at cm; drainage pipes (4) are installed at the adsorption layer positions on both sides of the road, with a slope of 4% to 5% for the drainage pipes (4), and anchor rods (6) are reserved between each layer of gabions; the spacing between drainage pipes (4) is 0.5 to 1.2 m; the spacing between anchor rods (6) is 0.5 to 1.8 m; S4. Lay out the seepage-proof interlayer on both sides of the roadbed (3); S5. Cover the surface of the seepage-proof interlayer (3) with a layer of geotextile, and then fill the soil to form the slope backfill (5). S6. Drainage pipe (4) Install drainage switch; inject nutrients and bacteria into the roadbed.

2. The bacterial-based mortise and tenon assembly gradient water purification and storage embankment construction method according to claim 1, characterized in that, In step S1, recycled aggregate with a particle size range of 5-25 mm is screened and selected; construction waste with excessively large particle size is crushed and then screened again; the prefabricated gabion is made of low-carbon steel wire with a metal mesh on the surface, and the mesh is a multi-stranded hexagonal shape; the woven bag is made of PET polyester woven bag.

3. The bacterial-based mortise and tenon assembly gradient water purification and storage embankment construction method according to claim 1, characterized in that, In S2, the sludge accounts for no more than 30% of the volume of the recycled aggregate.

4. The method for constructing a bacteria-based mortise and tenon joint assembled gradient water purification and storage roadbed according to claim 1, characterized in that, If the road surface is impermeable, a seepage ditch with a width of 0.7 to 0.9 m and a depth of 0.4 to 0.5 m should be reserved on the uppermost layer of the roadbed (2).

5. The method for constructing a bacteria-based mortise and tenon joint assembled gradient water purification and storage roadbed according to claim 1, characterized in that, The seepage-proof interlayer (3) in S4 is a reinforced concrete panel with a thickness of 6 to 10 cm, which is anchored to the gabion inside the roadbed and between the soil on both sides using anchor rods (6); the seepage-proof interlayers (3) on both sides of the roadbed share a common anchor rod (6).

6. The method for constructing a bacteria-based mortise and tenon joint assembled gradient water purification and storage roadbed according to claim 1, characterized in that, The slope of the slope fill (5) in S5 is 1:1 to 1:1.5; the slope surface is mainly planted with herbaceous or vine plants; shrubs and trees are planted as a supplement.

7. The method for constructing a bacteria-based mortise and tenon joint assembled gradient water purification and storage roadbed according to claim 1, characterized in that, The S6 injects nutrients into the roadbed. The nutrients include one or more of water, carbon source, energy source, nitrogen source, inorganic salts and growth factors. The bacteria include one or more combinations of Escherichia coli, Staphylococcus, Pasteurella multocida, Pasteurella megaterium, and Bacillus megaterium. The energy source includes glucose or agar.

8. A bacteria-based mortise and tenon joint assembled gradient water purification and storage roadbed, characterized in that, Construction shall be carried out using any of the construction methods described in claims 1 to 7.

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