An agricultural insecticide containing cyantraniliprole and spinetoram

Abstract

The application discloses an agricultural insecticide containing diafenthiuron and spinetoram, and belongs to the technical field of pesticides. The mass ratio of diafenthiuron and spinetoram in the agricultural insecticide is 0.1:25-1.0:10; the agricultural insecticide further comprises alkyl polyglycoside, and the addition amount of the alkyl polyglycoside is 5% in terms of mass percentage. The agricultural insecticide provided by the application has a control effect on lepidopteran pests, rice leaf roller, homopteran pests, tomato whitefly and thrips, and through indoor toxicity determination of the agricultural insecticide, it is found that when the mass ratio of diafenthiuron and spinetoram is 0.1:15-0.7:10, the co-toxicity coefficients of the agricultural insecticide on the tomato whitefly are all greater than 120, and the agricultural insecticide shows a synergistic effect. The application provides an agricultural insecticide with better effect for the control of crop pests.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology and relates to an agricultural insecticide containing bispyribac-methyl and spinosad. Background Technology

[0002] Afidopyropen is a biopesticide belonging to the vanillin receptor subfamily of channel modulators. It interferes with the regulation of the vanillic acid transient receptor channel complex in target insects, causing them to lose their sensitivity to gravity, balance, sound, position, and movement. This leads to loss of coordination and orientation, resulting in inability to feed, dehydration, and ultimately, starvation and death. Afidopyropen can reduce viral and bacterial diseases transmitted by insect vectors. It has low toxicity to mammals, fish, birds, bees, and predatory insects, and is safe for non-target animals and terrestrial plants.

[0003] Ethyl spinosad, also known as ethyl spinosad, is a spinosad insecticide produced by Dow AgroSciences. It is produced through fermentation by the actinomycete *Spinosarcina* and is a mixture of ethyl spinosad-J and ethyl spinosad-L. It is a white solid with a musty odor. Ethyl spinosad acts on nicotinic acetylcholine receptors and γ-aminobutyric acid receptors in the insect's nervous system, causing the insect to become insensitive to excitatory or inhibitory signal transmission, affecting normal neural activity, and ultimately leading to death. It degrades relatively quickly in the field and has low toxicity to birds, fish, earthworms, and aquatic plants.

[0004] In recent years, the application area of ​​insecticides has been continuously expanding. While single-variety insecticides are effective in controlling certain pests, they also exhibit strong selectivity, narrow application range, and short duration of action. Furthermore, long-term use can lead to pesticide resistance in pests. Therefore, providing a broad-spectrum, long-lasting agricultural insecticide is of great significance. Summary of the Invention

[0005] To address the problems of single-component insecticides having strong selectivity for pests, narrow application range, short duration of effect, and the development of pesticide resistance with long-term use, this invention provides an agricultural insecticide containing bifenthrin and spinosad. This agricultural insecticide, composed of bifenthrin and spinosad, also contains alkyl polyglycosides and is effective against common Thysanoptera, Homoptera, and Lepidoptera pests. This invention provides a more effective agricultural insecticide for the control of crop pests.

[0006] To achieve the technical objective of this invention, on one hand, this invention provides an agricultural insecticide composed of tebufenozide and spinosad, wherein the mass ratio of tebufenozide to spinosad in the agricultural insecticide is 0.1:25 to 1.0:10; the agricultural insecticide also includes alkyl polyglycosides, wherein the amount of alkyl polyglycosides added is 5% by mass percentage.

[0007] Furthermore, in the agricultural insecticide provided by the present invention, the mass ratio of tebufenozide to spinosad is 0.1:15 to 0.7:10. Preferably, the mass ratio of tebufenozide to spinosad is 0.1:10.

[0008] Furthermore, through indoor toxicity testing of agricultural insecticides, this invention found that when the mass ratio of bispyribac-toluamide to spinosad was 0.1:15 to 0.7:10, the co-toxicity coefficient of the agricultural insecticide against tomato whiteflies was greater than 120, demonstrating a synergistic effect. Among them, the co-toxicity coefficient was the largest at a mass ratio of bispyribac-toluamide to spinosad of 0.1:10, which was 166.34, indicating the best synergistic effect.

[0009] Furthermore, through field trials of agricultural insecticides, this invention found that insecticides containing tebuconazole and spinosad showed high control efficacy against rice leaf folder (87.64–98.51%) 15 days after application, which was higher than the control efficacy of single-agent application (65.46% and 77.81%). Insecticides containing tebuconazole and spinosad also showed high control efficacy against thrips (90.48–98.24%) 3 days after application, which was higher than the control efficacy of single-agent application (38.26% and 84.45%). This indicates that the combined use of tebuconazole and spinosad improves the control efficacy against both rice leaf folder and thrips.

[0010] Furthermore, through field trials of agricultural insecticides, this invention found that under the same effective dosage of bispyribac-methyl and spinosad, the addition of different surfactants produced different control effects on the insecticides. The agricultural insecticide with added alkyl polyglycosides was more effective against rice leaf folder than the experimental group with added Tween-80, OP-10, or alkylphenol polyoxyethylene ether, indicating that the agricultural insecticide with added alkyl polyglycosides provided by this invention has better control effect.

[0011] On the other hand, this invention claims protection for the use of the aforementioned agricultural insecticides in controlling lepidopteran, homoptera, and tsioptera pests. Lepidopteran pests include the rice leaf roller; homoptera pests include the tomato whitefly; and tsioptera pests include thrips.

[0012] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0013] This invention provides an agricultural insecticide containing tebufenozide and spinosad, which is effective against homoptera pests, and exhibits a synergistic effect when used together. Indoor toxicity tests of the agricultural insecticide revealed that when the mass ratio of tebufenozide to spinosad is 0.1:15 to 0.7:10, the co-toxicity coefficient against tomato whiteflies is greater than 120, demonstrating a synergistic effect. The highest co-toxicity coefficient (166.34) is achieved when the mass ratio of tebufenozide to spinosad is 0.1:10, indicating the optimal synergistic effect.

[0014] This invention provides an agricultural insecticide containing tebufenozide and spinosad, which is effective against lepidopteran and tsanoptera pests. Field trials of this agricultural insecticide revealed that the insecticide containing tebufenozide and spinosad showed a high control efficacy against rice leaf folder (87.64–98.51%) 15 days after application, higher than the control efficacy of single-agent application (65.46% and 77.81%). Furthermore, the insecticide containing tebufenozide and spinosad showed a high control efficacy against thrips (90.48–98.24%) 3 days after application, higher than the control efficacy of single-agent application (38.26% and 84.45%). This indicates that the combined use of tebufenozide and spinosad improves the control efficacy against rice leaf folder and thrips.

[0015] This invention provides an agricultural insecticide containing bispyribac-methyl and spinosad, wherein the added surfactant alkyl polyglycoside enhances the insecticide's efficacy. Field trials of this agricultural insecticide revealed that, under the same effective dosage of bispyribac-methyl and spinosad, the addition of different surfactants produced different control effects. The agricultural insecticide with added alkyl polyglycoside showed higher control efficacy against rice leaf folder than the groups treated with Tween-80, OP-10, or alkylphenol polyoxyethylene ether, indicating that the agricultural insecticide with added alkyl polyglycoside provided by this invention has better control efficacy. Detailed Implementation

[0016] The technical solution of the present invention will be described below with reference to embodiments. However, the present invention is not limited to the following embodiments. Unless otherwise specified, the experimental methods and detection methods described in each embodiment are conventional methods; unless otherwise specified, the reagents and materials can be purchased commercially.

[0017] Biprofen was purchased from Shaanxi Yikunte Pharmaceutical Technology Co., Ltd., with a purity of 97%.

[0018] Ethyl spinosad was purchased from Shenzhen Zhenqiang Biotechnology Co., Ltd., with a purity of 98%.

[0019] Alkyl polyglycosides were purchased from Shanghai Xinfan Biotechnology Co., Ltd.

[0020] Example 1

[0021] This embodiment provides a formulation of an agricultural insecticide consisting of a combination of bispyribac-methyl and spinosad.

[0022] 1. Suspension agent

[0023] By weight, every 100 parts of the suspension containing bispyribac-methyl and spinosad contains the following: 0.52 parts bispyribac-methyl, 15.91 parts spinosad, 5 parts alkyl polyglycoside, 5 parts calcium dodecylbenzenesulfonate, 3 parts alkylnaphthalene sulfonate, 0.5 parts glycerol, 0.8 parts silica, and water to make up the balance.

[0024] Weigh each component according to the above mass percentages, add bispyribac-methyl, spinosad, alkyl polyglycosides, calcium dodecylbenzenesulfonate, alkylnaphthalene sulfonate, and silica to a grinding kettle, grind to a certain fineness, then transfer the material to a mixing kettle, add the remaining glycerol and water, and mix thoroughly to obtain a 15.5% suspension containing bispyribac-methyl and spinosad.

[0025] Example 2

[0026] This embodiment provides an indoor toxicity test for agricultural insecticides.

[0027] The formulation used in this embodiment is a suspension, and the preparation method is the same as in Example 1. Each single agent and the mixture are prepared in equal proportions to form 5 series of mass concentrations.

[0028] The experiment involved 14 treatments: Experiment 1: Biprofen only; Experiment 2: Ethyl spinosad only; Experiment 3: Biprofen and ethyl spinosad in a mass ratio of 0.1:25; Experiment 4: Biprofen and ethyl spinosad in a mass ratio of 0.1:20; Experiment 5: Biprofen and ethyl spinosad in a mass ratio of 0.1:15; Experiment 6: Biprofen and ethyl spinosad in a mass ratio of 0.1:13; Experiment 7: Biprofen and ethyl spinosad in a mass ratio of 0.1:12; Experiment 8: Biprofen and... The mass ratio of ethyl spinosad was 0.1:11 in Experiment 9; the mass ratio of bispyribac-methyl to ethyl spinosad was 0.1:10 in Experiment 10; the mass ratio of bispyribac-methyl to ethyl spinosad was 0.2:10 in Experiment 11; the mass ratio of bispyribac-methyl to ethyl spinosad was 0.3:10 in Experiment 12; the mass ratio of bispyribac-methyl to ethyl spinosad was 0.5:10 in Experiment 13; the mass ratio of bispyribac-methyl to ethyl spinosad was 0.7:10 in Experiment 14; and the mass ratio of bispyribac-methyl to ethyl spinosad was 1.0:10 in Experiment 14.

[0029] Tomato whitefly nymphs were collected from a tomato planting base in Zhongshan Village, Xianyang, Shaanxi Province. The collected nymphs were stabilized on tomato leaves for 2–3 days under a constant temperature of 25±1℃. Nymphs with strong viability, similar weight, and consistent growth were selected as the source for bioassay. 30 μL of different concentrations of insecticide were applied to the prothorax of the tested tomato whitefly nymphs. Each concentration gradient constituted one treatment, with 10 nymphs per treatment and 3 replicates per treatment. The treated nymphs were placed in rearing boxes with fresh tomato leaves. After 24 hours under a constant temperature of 25±1℃, the survival and mortality of the nymphs were assessed (the nymphs were considered dead after being gently moved with a brush; no movement was recorded). The toxicity regression equation and LC-1 were calculated. 50 The co-toxicity coefficient was calculated using the Sun Yunpei method, with bispyribac-methyl as the standard agent and its toxicity index (TI) set at 100. The calculation results are shown in Table 1.

[0030]

[0031]

[0032] TTI = TI of the standard drug × percentage of the standard drug in the mixture + TI of ethyl spinosad × percentage of drug B in the mixture

[0033]

[0034] In the formula, ATI is the measured toxicity index of the mixture, TTI is the theoretical toxicity index of the mixture, and CTC is the co-toxicity coefficient of the compound. The synergistic effect of the mixture is evaluated by the CTC value: a CTC value ≥ 120 indicates a synergistic effect, a CTC value ≤ 80 indicates an antagonistic effect, and a CTC value < 80 < 100 indicates an additive effect.

[0035] Table 1: Indoor toxicity test results of insecticides containing bispyribac-methyl and spinosad against tomato whiteflies.

[0036] Experiment 1 Biprofen (A) 0.25 100 - - - Experiment 2 Ethyl spinosad (B) 3.56 7.02 - - - Experiment 3 A:B = 0.1:25 2.54 - 9.84 9.35 105.24 Experiment 4 A:B = 0.1:20 2.08 - 12.02 10.56 113.53 Experiment 5 A:B = 0.1:15 1.65 - 15.15 11.88 127.53 Experiment 6 A:B = 0.1:13 1.22 - 20.49 15.35 133.49 Experiment 7 A:B = 0.1:12 0.83 - 30.12 21.17 142.28 Experiment 8 A:B = 0.1:11 0.54 - 46.30 30.20 153.31 Experiment 9 A:B = 0.1:10 0.23 - 108.70 65.35 166.34 Experiment 10 A:B = 0.2:10 0.18 - 138.89 87.73 158.32 Experiment 11 A:B = 0.3:10 0.17 - 147.06 95.24 154.41 Experiment 12 A:B = 0.5:10 0.24 - 104.17 72.76 143.17 Experiment 13 A:B = 0.7:10 0.33 - 75.76 58.53 129.44 Experiment 14 A:B = 1.0:10 0.65 - 17.66 15.47 114.16

[0037] As shown in Table 1, when the mass ratio of bispyribac-toluamide to spinosad was 0.1:15 to 0.7:10, the co-toxicity coefficient was greater than 120, indicating a synergistic effect. Among them, the co-toxicity coefficient was the largest when the mass ratio of bispyribac-toluamide to spinosad was 0.1:10, which was 166.34, indicating the best synergistic effect.

[0038] Example 3

[0039] This embodiment provides a field trial of an agricultural insecticide.

[0040] 1. Field trials of the efficacy of agricultural insecticides against rice leaf roller

[0041] Based on the indoor toxicity tests, the optimal mass ratio of bispyribac-to-spinosad (0.1:15–0.7:10) for the combination of bispyribac-to-spinosad and spinosad was determined. Further field efficacy trials were conducted on the rice leaf roller as the target pest. The formulation used in this example is a suspension concentrate, prepared according to the method described in Example 1, diluted 5000 times before use, at a dosage of 50 kg / mu.

[0042] The experiment involved 16 treatments: Experiment 15: Biprofen and Sporotin at a mass ratio of 0.1:15; Experiment 16: Biprofen and Sporotin at a mass ratio of 0.1:13; Experiment 17: Biprofen and Sporotin at a mass ratio of 0.1:12; Experiment 18: Biprofen and Sporotin at a mass ratio of 0.1:11; Experiment 19: Biprofen and Sporotin at a mass ratio of 0.1:10; Experiment 20: Biprofen and Sporotin at a mass ratio of 0.2:10; Experiment 21: Biprofen and Sporotin at a mass ratio of 0.2:10; The mass ratio of spinosad to ethyl spinosad was 0.3:10; Experiment 22: The mass ratio of bispyribac-methyl to ethyl spinosad was 0.5:10; Experiment 23: The mass ratio of bispyribac-methyl to ethyl spinosad was 0.7:10; Experiment 24: Only bispyribac-methyl was added; Experiment 25: Only ethyl spinosad was added; Experiment 26: No insecticide was added, serving as a blank control group; Experiment 27: No alkyl polyglycosides were added; Experiment 28: Alkyl polyglycosides were replaced with an equal amount of Tween-80; Experiment 29: Alkyl polyglycosides were replaced with an equal amount of OP-10; Experiment 30: Alkyl polyglycosides were replaced with an equal amount of alkylphenol polyoxyethylene ether.

[0043] The experiment was conducted on machine-transplanted late-season rice varieties, specifically Jiayou No. 2, where rice leaf roller infestation was severe and a significant proportion of middle-instar larvae were present. Before the experiment, ridges were prepared and plots were divided into sections, each 20 m² in size. 2 Each treatment was replicated four times in a randomized block design. A backpack sprayer was used for uniform spraying. The application time was mid-September. Before application, the insect population was investigated using the basin tapping method. The number of live rice leaf rollers was investigated at 7, 15 and 30 days after application. Five sampling points were taken in each plot. Three leaves were fixed in the upper part of the rice plant at each point. The number of adult rice leaf rollers on each leaf was recorded, and the control effect was calculated.

[0044]

[0045] In the formula, PT0 is the number of insects in the pesticide-treated area before application; PT1 is the number of insects in the pesticide-treated area after application; CK0 is the number of insects in the blank control area before application; and CK1 is the number of insects in the blank control area after application.

[0046] Table 2: Field trial results of insecticides containing bispyribac-methyl and spinosad against rice leaf roller.

[0047]

[0048] Table 2 shows that the insecticide containing tebufenozide and spinosad provided by this invention exhibits high control efficacy against rice leaf folder 15 days after application, ranging from 87.64% to 98.51%, which is higher than the control efficacy of 65.46% and 77.81% when used alone. This indicates that the combined use of tebufenozide and spinosad improves the control efficacy against rice leaf folder. Under the same effective dosage of tebufenozide and spinosad, the addition of different surfactants produces different control effects. The agricultural insecticide with added alkyl polyglycosides shows higher control efficacy against rice leaf folder than the experimental group with added Tween-80, OP-10, or alkylphenol polyoxyethylene ether. Furthermore, the control effect of the treatment group with added surfactants is better than that of the group without added surfactants, indicating that the agricultural insecticide with added alkyl polyglycosides provided by this invention has better control efficacy.

[0049] 2. Field trials of the efficacy of agricultural insecticides against rice leaf roller.

[0050] Based on the indoor toxicity tests, the optimal mass ratio of bispyribac-to-spinosad (0.1:15–0.7:10) for the combination of bispyribac-to-spinosad and spinosad was determined. Further field efficacy trials were conducted on thrips on cowpeas as the target pest. The formulation used in this example is a suspension concentrate, prepared according to Example 1, diluted 5000 times, and applied at a rate of 50 kg / mu.

[0051] The experiment involved 16 treatments: Experiment 31: Biprofen and Sporotin at a mass ratio of 0.1:15; Experiment 32: Biprofen and Sporotin at a mass ratio of 0.1:13; Experiment 33: Biprofen and Sporotin at a mass ratio of 0.1:12; Experiment 34: Biprofen and Sporotin at a mass ratio of 0.1:11; Experiment 35: Biprofen and Sporotin at a mass ratio of 0.1:10; Experiment 36: Biprofen and Sporotin at a mass ratio of 0.2:10; Experiment 37: Biprofen and Sporotin at a mass ratio of 0.2:10; The mass ratio of spinosad to ethyl spinosad was 0.3:10; Experiment 38: The mass ratio of bispyribac-methyl to ethyl spinosad was 0.5:10; Experiment 39: The mass ratio of bispyribac-methyl to ethyl spinosad was 0.7:10; Experiment 40: Only bispyribac-methyl was added; Experiment 41: Only ethyl spinosad was added; Experiment 42: No insecticide was added, serving as a blank control group; Experiment 43: No alkyl polyglycosides were added; Experiment 44: Alkyl polyglycosides were replaced with an equal amount of Tween-80; Experiment 45: Alkyl polyglycosides were replaced with an equal amount of OP-10; Experiment 46: Alkyl polyglycosides were replaced with an equal amount of alkylphenol polyoxyethylene ether.

[0052] The experiment was conducted in a cowpea area with severe thrips infestation, using the cowpea variety Lianjiang No. 1 as the experimental field, with a plot size of 20m². 2 Each treatment was replicated four times, arranged in randomized blocks with a guard row. A backpack sprayer was used to spray the cowpeas during their peak flowering period. More than 30 flowers were randomly selected from the upper, middle and lower parts of each plot. The number of live thrips was investigated before application and at 3, 5 and 7 days after application to calculate the control effect.

[0053]

[0054] In the formula, PT0 is the number of insects in the pesticide-treated area before application; PT1 is the number of insects in the pesticide-treated area after application; CK0 is the number of insects in the blank control area before application; and CK1 is the number of insects in the blank control area after application.

[0055] Table 3: Field trial results of insecticides containing bispyribac-methyl and spinosad against thrips

[0056]

[0057]

[0058] Table 3 shows that the insecticide containing bispyribac-methyl and spinosad provided by this invention exhibits a high control efficacy against thrips (90.48–98.24%) 3 days after application, which is higher than the control efficacy of single-agent application (38.26% and 84.45%). This indicates that the combined use of bispyribac-methyl and spinosad improves the control efficacy against thrips. Under the same effective dosage of bispyribac-methyl and spinosad, the addition of different surfactants produces different control effects. The agricultural insecticide with added alkyl polyglycosides shows higher control efficacy against rice leaf folder than the experimental group with added Tween-80, OP-10, or alkylphenol polyoxyethylene ether. Furthermore, the control effect of the treatment group with added surfactants is better than that of the group without added surfactants, indicating that the agricultural insecticide with added alkyl polyglycosides provided by this invention has better control efficacy.

[0059] The embodiments described above are some, but not all, of the embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art through related deductions and substitutions based on the inventive concept, without inventive effort, are within the scope of protection of the present invention.

Claims

1. An agricultural insecticide, characterized by comprising, It is composed of dipropionate and ethyl spinosad; The mass ratio of bispyribac-methyl to spinosad is 0.1:15~0.7:10; The agricultural insecticide also includes alkyl polyglycosides, with the amount of alkyl polyglycosides added being 5% by mass percentage.

2. The agricultural insecticide of claim 1, wherein, The mass ratio of bispyribac-methyl to spinosad in the agricultural insecticide is 0.1:

10.

3. The application of the agricultural insecticide according to any one of claims 1 to 2 in the control of lepidopteran pests, homoptera pests and tsioptera pests.

4. The application according to claim 3, characterized in that, The Lepidoptera pests include the rice leaf roller; the Homoptera pests include the tomato whitefly; and the Thysanoptera pests include the thrips.

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