A pesticide composition for controlling aphids on wormwood
By combining rotenone with pesticide components such as dipropionate, the problems of unsatisfactory aphid control and excessive pesticide residues in existing technologies have been solved, achieving efficient control and environmentally friendly application, and improving the quality of Artemisia argyi.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- NANYANG ACAD OF AGRI SCI
- Filing Date
- 2022-11-21
- Publication Date
- 2026-06-09
Smart Images
Figure BDA0004657816980000031 
Figure BDA0004657816980000032 
Figure BDA0004657816980000041
Abstract
Description
Technical Field
[0001] This invention belongs to the field of pesticide technology, specifically to an insecticide composition for controlling Artemisia aphids. Background Technology
[0002] Artemisia argyi is a perennial herbaceous plant belonging to the Asteraceae family, also known as mugwort, wormwood, and fragrant mugwort. The whole plant is used medicinally, possessing various medicinal values such as dispelling cold, stopping bleeding, reducing inflammation, relieving cough, and anti-allergy. With increasing demand for health and wellness, moxibustion, a traditional Chinese medicine therapy, has gained acceptance among patients with chronic diseases, leading to a year-on-year increase in market demand for artemisia argyi. Like many crops, artemisia argyi is susceptible to pests during cultivation. Common pests include aphids, mites, and whiteflies. Among these, the most common aphid is the peach gall aphid, which primarily damages artemisia argyi in its adult and nymphal stages. These aphids often congregate on new shoots and tender leaves, sucking sap and reducing photosynthesis, causing the leaves to curl and turn yellow, thus affecting the normal growth of artemisia argyi.
[0003] Chemical pesticides are a common method for controlling agricultural pests and diseases. Spraying chemical pesticides during the cultivation of Artemisia argyi can effectively control the aphid, the peach gall aphid. However, due to long-term use of single pesticides and unscientific application methods, resistance has developed in the peach gall aphid, making the control effect of many existing chemical pesticides less than ideal. Increasing the amount of pesticides used can easily lead to excessive pesticide residues in Artemisia argyi, affecting its quality. Therefore, it is necessary to provide a pesticide that requires low dosage and is highly effective in controlling Artemisia argyi aphids.
[0004] Rotenone is widely found in the root bark of plants and has strong contact and stomach poison effects on insects, especially cabbage white butterflies, diamondback moths, thrips, and aphids. Once inside the insect's body, rotenone inhibits the mitochondrial respiratory chain, leading to systemic cellular hypoxia, respiratory failure, cardiac arrest, and death. It possesses unique potential for controlling resistant pests. Combining different pesticide components is an effective way to develop new agents; however, there are currently no reports of rotenone being combined with tebuconazole, chlorfenapyr, pymetrozine, or azoxystrobin. Summary of the Invention
[0005] The purpose of this invention is to provide a pesticide composition for controlling Artemisia aphids, which can improve the control effect on Artemisia pests such as peach aphids, reduce the dosage of pesticides, facilitate the achievement of pollution-free control in the cultivation of Artemisia, and improve the quality of Artemisia.
[0006] To achieve the above objectives, the present invention provides the following technical solution:
[0007] An insecticide composition for controlling Artemisia aphids, wherein the active ingredient is composed of rotenone and compound a, wherein compound a is dipropionate.
[0008] More specifically, when compound a is diproterenol, the mass ratio of rotenone to compound a is 1-40:10-1.
[0009] The present invention also provides a pesticide formulation comprising the aforementioned pesticide composition for controlling Artemisia aphids.
[0010] More specifically, the pesticide formulation is in the form of wettable powder, suspension concentrate, ultra-low volume liquid, or water-dispersible granules.
[0011] Compared with the prior art, the present invention has the following beneficial effects:
[0012] (1) The insecticide composition for controlling Artemisia aphids of the present invention has a synergistic effect on controlling Artemisia aphids, which can improve the control effect on Artemisia aphids, help reduce the dosage of pesticides, reduce the amount of pesticide residues in Artemisia and reduce environmental pollution, and help achieve the goal of pollution-free control in the planting process of Artemisia and improve the quality of Artemisia.
[0013] (2) The two active ingredients in the insecticide composition for controlling Artemisia aphids of the present invention have different mechanisms of action on pests, which helps to delay the emergence and development of pesticide resistance in pests, and thus can extend the service life of pesticides and extend the replacement cycle to a certain extent. Detailed Implementation
[0014] The present invention can be better understood from the following embodiments. The description of the embodiments is for illustrative purposes only and should not and will not limit the present invention as described in detail in the claims.
[0015] Example Indoor bioactivity test
[0016] 1. Experimental subjects: Peach aphids were collected from the Artemisia argyi planting area and fed with fresh and clean Artemisia argyi tender leaves in the laboratory. Nymphs were selected as experimental subjects.
[0017] 2. Test reagents
[0018] The following pesticides are available commercially: 95% rotenone technical grade, 92.5% difenoconazole technical grade, 98% chlorfenapyr technical grade, 97% pymetrozine technical grade, and 98% azoxystrobin technical grade.
[0019] 3. Test methods (Refer to NY / T 1154.6-2006 Guidelines for Indoor Bioassay of Pesticides - Part 6: Insecticidal Activity Test - Immersion Method)
[0020] The test reagent was dissolved and then diluted with 0.1% Tween-80 solution to prepare single-agent stock solutions. Multiple ratios were set up, and five gradient mass concentrations were set for each single agent and each ratio mixture according to the proportional method. The volume of the drug solution for each mass concentration was 50 mL. All reagents were prepared and used immediately.
[0021] The test worms were immersed in the drug solution for 8 seconds, and excess solution was absorbed with filter paper before being transferred to normal rearing conditions. Each treatment was repeated 4 times, with 25 worms immersed in each replicate. A control group containing only 0.1% Tween-80 was included. The mortality of the test worms was observed 24 hours after treatment, and the total number of worms and the number of dead worms in each treatment were recorded. The corrected mortality rate for each treatment was then calculated.
[0022]
[0023] In the above formula: P -- mortality rate, in %; K -- number of dead insects; N -- total number of insects treated.
[0024]
[0025] In the above formula: P1 -- corrected mortality rate, in %; P t --Treatment mortality rate, in %; P0--Control mortality rate, in %.
[0026] 4. Data Analysis: DPS software was used to perform regression analysis on the logarithmic values of the drug concentrations for each treatment and the corrected mortality probability values for each treatment, and the LC ratio of the drug for each treatment was calculated. 50 The co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method.
[0027]
[0028] In the above formula: ATI -- the measured toxicity index of the mixture; S -- the LC of the standard reagent. 50 Units are mg / L; M -- LC50 of the mixture 50 The unit is mg / L.
[0029] TTI = TI A ×P A +TI B ×P B
[0030] In the above formula: TTI -- theoretical toxicological index of the mixture; TI A --Toxicity index of drug A; P A --Percentage content of drug A in the mixture, expressed as percentage (%); TI B --Toxicity index of drug B; P B --The percentage content of drug B in the mixture, expressed as a percentage (%).
[0031]
[0032] In the above formula: CTC -- co-toxicity coefficient; ATI -- measured toxicity index of the mixture; TTI -- theoretical toxicity index of the mixture.
[0033] 5. Drug efficacy evaluation
[0034] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The results are shown in Table 1-4.
[0035] Table 1. Toxicity test results of rotenone and diproterenol combined with *Tetranychus sylvestris* on *Tetranychus sylvestris*.
[0036]
[0037]
[0038] As shown in Table 1, within a mass ratio of 1-40:10-1, the co-toxicity coefficient of rotenone and diproterenol against peach aphid is greater than 120, indicating a synergistic effect.
[0039] Table 2. Results of toxicity assays of rotenone and chlorfenapyr combined with *Gnaphalium affine*.
[0040]
[0041] As shown in Table 2, within a mass ratio of 1-20:1, the co-toxicity coefficient of rotenone and chlorfenapyr against peach aphid is greater than 120, indicating a synergistic effect.
[0042] Table 3. Results of toxicity assays of rotenone and pymetrozine combination against *Aphis pulveratum*.
[0043]
[0044]
[0045] As shown in Table 3, within a mass ratio of 1-80:15-1, the co-toxicity coefficient of rotenone and pymetrozine against peach gall aphid is greater than 120, indicating a synergistic effect.
[0046] Table 4. Toxicity test results of rotenone and acetamiprid combined with *Aphis pulveratum*.
[0047]
[0048] As shown in Table 4, within a mass ratio of 1-20:9-1, the co-toxicity coefficient of rotenone and acetamiprid against peach aphid is greater than 120 after being combined, indicating a synergistic effect.
[0049] In summary, the pesticide composition for controlling Artemisia aphids of the present invention has a synergistic effect on controlling Artemisia aphids, improving the control effect on Artemisia aphids, helping to reduce the dosage of pesticides, reduce pesticide residues in Artemisia, and reduce environmental pollution. This is conducive to achieving the goal of pollution-free control in the cultivation of Artemisia and improving the quality of Artemisia.
[0050] The above description is only a preferred embodiment of the present invention. For those skilled in the art, appropriate improvements can be made without departing from the principle of the present invention, and these improvements are also within the protection scope of the present invention.
Claims
1. The application of an insecticide composition in controlling the aphids *Artemisia annua* and *Aphidius sylvestris*, characterized in that, The active ingredient of the insecticide composition is composed of rotenone and compound a, wherein compound a is dipropionate; the mass ratio of rotenone to compound a is 1-40:10-1.
2. A pesticide formulation, characterized in that, Includes the insecticide composition according to claim 1.
3. The pesticide formulation according to claim 2, characterized in that, The pesticide formulation is in the form of wettable powder, suspension concentrate, ultra-low volume liquid, or water-dispersible granules.