Application of small molecule organic acids in promoting absorption and transportation of pesticides by crops

Abstract

The application discloses application of small-molecule organic acids in promoting absorption and / or transportation of a pesticide thifluzamide by crops, and in particular, the small-molecule organic acid is succinic acid, citric acid or tryptophan. The small-molecule organic acid is mixed with the pesticide in a rice nutrient solution, and the pesticide is applied to the root of the rice, so that the content of the pesticide in the root area and the stem and leaves of the rice is significantly increased, the absorption and transportation of the pesticide by the rice are promoted, and in turn, the utilization rate of the pesticide by the rice is greatly improved. The method provided by the application is simple in operation and green in environment protection, and has high application value in promoting absorption of the pesticide by crops, improving the prevention and treatment effect of the pesticide on crop diseases and insect pests, reducing the pesticide dosage, and in turn, guaranteeing agricultural production, agricultural product quality and ecological environment safety.

Description

Technical Field

[0001] This invention belongs to the field of biological control technology. More specifically, it relates to the application of small molecule organic acids in promoting the absorption and transport of pesticides by crops. Background Technology

[0002] Pesticide control is one way to ensure the safety of agricultural production, significantly reducing the incidence of pests and diseases and ensuring that crop yields are not affected. However, the extensive use of pesticides leads to pesticide residues in crops, soil, water, and other environmental environments, threatening human health, causing pests to develop resistance, and increasing control costs. Therefore, reducing pesticide use and improving pesticide utilization efficiency while ensuring the effectiveness of pesticide control, thereby safeguarding agricultural production, agricultural product quality, and the ecological environment, is a key issue that urgently needs to be addressed.

[0003] In existing technologies, improving the effective utilization rate of pesticides mainly involves increasing the amount of pesticide deposited on the crop surface by improving the type of pesticide formulation, optimizing the performance and configuration of plant protection machinery, application methods, and pesticide adjuvants, thereby improving the effective utilization rate of pesticides. However, these methods are either complex to implement and costly, or they only improve the effective utilization rate of pesticides to a limited extent.

[0004] Patent CN115462375A discloses the effect of compound amino acid powder on promoting pesticide absorption by crops; however, the composition of compound amino acid powder is complex, and the effective components cannot be clearly identified. Furthermore, studies have shown that different amino acids have significantly different effects on pesticide absorption.

[0005] Therefore, exploring molecules that have clear effects, low cost, and effectively enhance crop absorption of pesticides is of great significance for improving crop disease control, implementing the concept of green agricultural development, and reducing the negative impact of pesticide use on agricultural product quality and safety and ecological environment safety. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of existing technologies for improving the effective utilization rate of pesticides, and to provide an application of small molecule organic acids in promoting the absorption and transport of pesticides by crops. The small molecule organic acids are succinic acid, citric acid and tryptophan.

[0007] The first object of the present invention is to provide the use of small molecule organic acids in promoting the absorption and / or translocation of the pesticide thifluzamide by crops, and their use in the preparation of products that promote the absorption and / or translocation of the pesticide thifluzamide by crops.

[0008] The second objective of this invention is to provide the application of small molecule organic acids as synergists in the preparation of the pesticide thifluzamide, and the application of small molecule organic acids in combination with thifluzamide in the preparation of pesticide formulations.

[0009] A third objective of this invention is to provide the application of small molecule organic acids in promoting the absorption and translocation of pesticides by crops and enhancing the ability of crop roots to resist pesticide oxidative stress, as well as their application in the preparation of products that promote the absorption and translocation of pesticides by crops and enhance the ability of crop roots to resist pesticide oxidative stress.

[0010] The fourth objective of this invention is to provide a pesticide formulation.

[0011] A fifth objective of this invention is to provide a method for promoting the absorption and / or translocation of the pesticide thifluzamide by crops.

[0012] A sixth object of the present invention is to provide the application of the said pesticide formulation in improving the control of crop diseases or in the preparation of products that improve the control of crop diseases.

[0013] The above-mentioned objective of this invention is achieved through the following technical solution:

[0014] This invention involves mixing succinic acid, citric acid, or tryptophan with the pesticide thifluzamide and applying the mixture to the roots of rice. This significantly increases the pesticide content in the root zone and stems and leaves of rice, indicating that succinic acid, citric acid, or tryptophan promotes the absorption and translocation of pesticides by rice, thereby greatly improving the utilization rate of pesticides by rice.

[0015] Therefore, this invention seeks to protect the following applications:

[0016] The application of small molecule organic acids in promoting the absorption and / or transport of the pesticide thifluzamide by crops, wherein the small molecule organic acid is any one or more of succinic acid, citric acid or tryptophan.

[0017] The application of small molecule organic acids in the preparation of products that promote the absorption and / or translocation of the pesticide thifluzamide by crops, wherein the small molecule organic acid is any one or more of succinic acid, citric acid or tryptophan.

[0018] The application of small molecule organic acids in the preparation of synergists for the pesticide thifluzamide, wherein the small molecule organic acid is any one or several of succinic acid, citric acid, or tryptophan.

[0019] The application of small molecule organic acids combined with thifluzamide in the preparation of pesticide formulations, wherein the small molecule organic acids are any one or more of succinic acid, citric acid, or tryptophan.

[0020] Based on this, the present invention provides a pesticide formulation containing a small molecule organic acid and thifluzamide, wherein the small molecule organic acid is any one or more of succinic acid, citric acid or tryptophan.

[0021] Given that thifluzamide is effective against a variety of crop diseases, this invention also seeks to protect the use of the above-mentioned pesticide formulation in improving the control effect of crop diseases or in the preparation of products that improve the control effect of crop diseases, including sheath blight, white mold, and stem rot.

[0022] Furthermore, this invention also applies for protection of a method for promoting the absorption and / or translocation of the pesticide thifluzamide by crops, specifically the method of applying a small molecule organic acid and thifluzamide together to the roots of crops, wherein the small molecule organic acid is any one or more of succinic acid, citric acid, or tryptophan.

[0023] In the above method, preferably, the concentration of the small molecule organic acid is 0.01 to 10 mM.

[0024] More preferably, the concentration of the small molecule organic acid is 0.01 to 1 mM.

[0025] More preferably, the concentration of the small molecule organic acid is 0.1 to 1 mM.

[0026] In addition, the research data of this invention show that succinic acid and tryptophan can significantly reduce the malondialdehyde content in roots, succinic acid and citric acid can increase the activity of catalase, and tryptophan can increase the activity of superoxide dismutase, indicating that these three organic acids can enhance the ability of plant roots to resist pesticide oxidative stress.

[0027] Therefore, the scope of protection of this invention also includes: the application of small molecule organic acids in promoting the absorption and translocation of pesticides by crops and enhancing the ability of crop roots to resist pesticide oxidative stress, and the application of small molecule organic acids in the preparation of products that promote the absorption and translocation of pesticides by crops and enhance the ability of crop roots to resist pesticide oxidative stress, wherein the small molecule organic acid is any one or several of succinic acid, citric acid or tryptophan, and the pesticide is thifluzamide.

[0028] The present invention has the following beneficial effects:

[0029] 1. This invention is the first to discover that small-molecule organic acids such as succinic acid, citric acid, and tryptophan can promote the absorption and utilization of the pesticide thifluzamide by crops, reduce pesticide dosage, and improve the control effect of pesticides on crop diseases and pests. Simultaneously, these small-molecule organic acids can also enhance the ability of plant roots to resist pesticide oxidative stress. The technical solution of this invention provides agricultural production with more effective pesticide utilization strategies and technologies, promoting sustainable agricultural development.

[0030] 2. The method of promoting crop absorption of pesticides by applying small molecule organic acids through root application provided by this invention is simple to operate, environmentally friendly, and has a more effective pesticide utilization efficiency. In actual production applications, it can reduce the amount of pesticides used and reduce pollution to farmland, and has important agricultural and environmental protection value. Attached Figure Description

[0031] Figure 1 The effects of different concentrations of succinic acid, citric acid, and tryptophan on the absorption of thifluzamide in rice.

[0032] Figure 2 : Concentration of thifluzamide in different parts of rice at different time points after the addition of 0.1 mM succinic acid. Different lowercase letters indicate significant differences in thifluzamide concentration between the control group and the treatment group at the same time point (p<0.05, t-test).

[0033] Figure 3 : Concentration of thifluzamide in different parts of rice at different time points after the addition of 0.1 mM citric acid. Different lowercase letters indicate significant differences in thifluzamide concentration between the control group and the treatment group at the same time point (p<0.05, t-test).

[0034] Figure 4 : Thifluzamide concentration in different parts of rice at different time points after the addition of 1 mM tryptophan. Different lowercase letters indicate significant differences in thifluzamide concentration between the control group and the treatment group at the same time point (p<0.05, t-test).

[0035] Figure 5 : Concentration of tebuconazole in different parts of rice at different time points after the addition of 0.1 mM citric acid. Different lowercase letters indicate significant differences in tebuconazole concentration between the control group and the treatment group at the same time point (p<0.05, t-test).

[0036] Figure 6 The effects of different small-molecule organic acids on the physiological indicators of rice roots. Detailed Implementation

[0037] The present invention will be further described below with reference to the accompanying drawings and specific embodiments, but the embodiments do not limit the present invention in any way. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in this technical field.

[0038] Unless otherwise specified, all reagents and materials used in the following examples are commercially available.

[0039] The thifluzamide suspension used in the following examples was purchased from Jinan Lvba Pesticide Co., Ltd., and the citric acid, succinic acid, and tryptophan used were purchased from Shanghai Maclean Biochemical Technology Co., Ltd. Kimura B rice nutrient solution was purchased from Guangzhou Daxin Biotechnology Co., Ltd. (sterilized by high-pressure steam before use).

[0040] The rice variety used in the following examples is Huahang 31 indica rice, purchased from Guangdong Huanongda Seed Industry Co., Ltd.

[0041] The rice cultivation methods used in the following examples are as follows:

[0042] (1) Before rice seeds germinate, use tap water flotation to remove shriveled seeds without endosperm, disinfect them by soaking in 2% sodium hypochlorite for 20 minutes, rinse them repeatedly with distilled water, and then soak them in distilled water in a 37℃ constant temperature box for 24 hours.

[0043] (2) Select seeds that have absorbed water and are showing white spots, place them in a petri dish lined with two layers of filter paper, add distilled water to partially immerse the seeds, and place them in a dark environment to promote germination and root growth.

[0044] (3) When the primary roots of the rice embryos reach 2-3 cm and have no lateral roots, transfer them to a light incubator. Pre-culture them in 1 / 2 Kimura B nutrient solution for 3 days, then replace with full Kimura B nutrient solution. Change the nutrient solution every 3-5 days. The light incubator parameters are set as follows: light intensity 300 μmol / m². 2 / s, photoperiod is 16h, temperature is 28℃ during illumination, temperature is 30℃ during darkness, and relative humidity is 80%.

[0045] The specific components of 1 / 2 Kimura B nutrient solution are shown in Table 1 below:

[0046] Table 1. Composition of 1 / 2 Kimura B Nutrient Solution

[0047]

[0048]

[0049] The specific composition of the complete Kimura B nutrient solution is shown in Table 2 below:

[0050] Table 2. Composition of the complete Kimura B nutrient solution

[0051]

[0052] Example 1: Effects of different concentrations of succinic acid, citric acid, and tryptophan on the absorption of thifluzamide in rice.

[0053] The specific experimental method is as follows:

[0054] 1. Select robust and uniformly growing rice seedlings and transfer them to brown glass bottles containing Kimura B rice nutrient solution with thifluzamide (wrap the bottles with aluminum foil and seal the openings). The initial concentration of thifluzamide in the rice nutrient solution is 40 mg / kg.

[0055] 2. Different concentrations of organic acids (succinic acid, citric acid, tryptophan) were added to each group. The group without added organic acids served as the control group. The final concentration of organic acids in each group was 0-10 mM. Each treatment was set up in 3 replicates.

[0056] 3. Five days after adding organic acid, samples were collected from rice roots and stems for pesticide (thifluzamide) content testing. Specific sample collection and testing methods are as follows:

[0057] (1) Extraction method of thifluzamide from rice roots: Accurately weigh 0.5 g of rice root sample into a 5 mL grinding tube, add 3 mL of chromatographic acetonitrile, shake for 1 min, and sonicate for 30 min. Add 1.0 g of sodium chloride, shake for 1 min, and centrifuge at 9000 rpm for 3 min. Take 1 mL of the supernatant after centrifugation into a 2 mL centrifuge tube containing 50 mg of PSA, shake for 1 min, and centrifuge at 10000 rpm for 5 min. Filter the supernatant after centrifugation through a 0.22 μm microporous organic filter membrane and collect it in a brown sample bottle for analysis.

[0058] (2) Extraction method of thifluzamide from rice stems and leaves: Accurately weigh 0.5 g of rice stem and leaf sample into a 5 mL grinding tube, add 3 mL of chromatographic acetonitrile, shake for 1 min, and sonicate for 30 min. Add 1.0 g of sodium chloride, shake for 1 min, and centrifuge at 9000 rpm for 3 min. Take 1 mL of the supernatant after centrifugation into a 2 mL centrifuge tube containing 50 mg of PSA and 10 mg of GCB, shake for 1 min, and centrifuge at 10000 rpm for 5 min. Filter the supernatant after centrifugation through a 0.22 μm microporous organic filter membrane and collect it in a brown sample bottle for analysis.

[0059] (3) Method for detecting thifluamide content:

[0060] The concentration of thifluzamide was determined using an Agilent 1260 high-performance liquid chromatograph under the following chromatographic conditions:

[0061] Chromatographic column: Athena C18, 120A, 4.6 × 250 mm, 5 μm liquid chromatography column

[0062] Mobile phase: 80% acetonitrile, 20% water

[0063] Flow rate: 0.800 mL / min

[0064] Column temperature: Not controlled

[0065] Detection was performed using a DAD detector: the detection wavelength was 206 nm, and the temperature was 24℃.

[0066] Determination of thifluzamide concentration in rice root and stem / leaf samples: A series of mixed standard solutions of 50, 10, 5, 1, and 0.5 mg / L were prepared by diluting a 100 mg / L thifluzamide standard solution with chromatographic acetonitrile. These solutions were then analyzed by high-performance liquid chromatography (HPLC) under the aforementioned chromatographic conditions. A standard curve was plotted with peak area on the ordinate and the concentration of the thifluzamide standard solution on the abscissa. The concentration of thifluzamide in the rice root and stem / leaf samples was determined by substituting the measured peak area values ​​into the standard curve equation.

[0067] The results are shown in the Experimental Results and Analysis section below.

[0068] Example 2: Effects of different treatment times with succinic acid, citric acid, and tryptophan on the absorption of thifluzamide in rice.

[0069] The specific experimental method is as follows:

[0070] 1. Select robust and uniformly growing rice seedlings and transfer them to brown glass bottles containing Kimura B rice nutrient solution with thifluzamide (wrap the bottles with aluminum foil and seal the openings). The initial concentration of thifluzamide in the rice nutrient solution is 40 mg / kg.

[0071] 2. Add 0.1mM succinic acid, 0.1mM citric acid, and 1mM tryptophan respectively. Set up a control group without added organic acids. Each treatment group has 3 replicates.

[0072] 3. Rice root and stem / leaf samples were collected 2 hours (0d), 1 day, and 3 days after the addition of organic acid for pesticide (thifluzamide) content detection. The specific sample collection and detection methods were the same as those described in Example 1.

[0073] The results are shown in the Experimental Results and Analysis section below.

[0074] Example 3: Effects of succinic acid, citric acid, and tryptophan on the physiological characteristics of rice roots

[0075] The specific experimental method is the same as in Example 2. On the third day after adding the organic acid, the physiological indicators of rice roots (malondialdehyde content, reduced glutathione content, catalase activity, and superoxide dismutase activity) were measured. The specific measurement method is as follows:

[0076] 1. Determination of malondialdehyde content in roots

[0077] (1) Accurately weigh 0.1g of root system, add 1mL of K2HPO4-KH2PO4 buffer (pH=7.8) for ice bath homogenization, and then centrifuge at 4℃ and 8500rpm for 10min. The supernatant obtained is malondialdehyde crude extract.

[0078] (2) Take 1 mL of malondialdehyde crude extract, add 2 mL of 0.6% TBA solution, mix thoroughly, keep warm in a 100℃ water bath for 60 min (cover tightly to prevent moisture loss), cool in an ice bath, and then centrifuge at room temperature for 10 min at 10000 rpm.

[0079] (3) Take 200 μL of the supernatant from step (2) and measure the absorbance of the supernatant sample at 450 nm, 532 nm and 600 nm using an ELISA reader. Determine and calculate the relative content of malondialdehyde (MDA) according to the instructions of the malondialdehyde (MDA) content detection kit (catalog number D799761-0050, Sangon Biotech (Shanghai) Co., Ltd.).

[0080] 2. Determination of reduced glutathione content in roots

[0081] (1) Accurately weigh 0.1g of root system, add 1mL of 5% metaphosphoric acid solution for ice bath homogenization, and then centrifuge at 4℃ and 8500rpm for 10min to obtain the supernatant as crude extract of reduced glutathione.

[0082] (2) Add each solution according to Table 3 and mix thoroughly (the samples in the table are crude reduced glutathione extracts). After mixing, let stand for 2 minutes and detect the absorbance of the mixture at 412 nm using an ELISA reader. Determine and calculate the relative content of reduced glutathione according to the instructions of the reduced glutathione (GSH) content assay kit (catalog number D799613-0050, Sangon Biotech (Shanghai) Co., Ltd.).

[0083] 3. Root catalase activity assay

[0084] (1) Accurately weigh 0.1g of root system, add 1mL of K2HPO4-KH2PO4 buffer (pH=7.0) for ice bath homogenization, and then centrifuge at 4℃ and 8500rpm for 10min. The supernatant obtained is the crude catalase extract.

[0085] (2) Take 10 μL of crude catalase extract and add it to 190 μL of mixed solution (the mixed solution is prepared by mixing 20 mL of 0.1 mol / L K2HPO4-KH2PO4 buffer and 5 mL of 0.1 mol / L H2O2). Mix immediately and start timing. Measure the initial absorbance A1 of the mixed sample at 240 nm and the absorbance A2 at 240 nm after 1 min using a microplate reader. Determine and calculate the relative activity of catalase according to the instructions of the catalase (CAT) activity assay kit (catalog number D799597-0050, Sangon Biotech (Shanghai) Co., Ltd.).

[0086] 4. Root superoxide dismutase activity assay

[0087] (1) Accurately weigh 0.1g of root system, add 1mL of K2HPO4-KH2PO4 buffer (pH=7.8) for ice bath homogenization, and then centrifuge at 4℃ and 8500rpm for 10min. The supernatant obtained is the crude extract of superoxide dismutase.

[0088] (2) Add the solutions according to Table 3 and mix thoroughly (the samples in the table are crude superoxide dismutase extracts). Incubate at 37°C for 30 min. After precipitation, take 200 μL of the supernatant and measure the absorbance of the supernatant sample at 560 nm using an ELISA reader. Determine and calculate the relative activity of superoxide dismutase according to the instructions of the superoxide dismutase (SOD) activity assay kit (catalog number D799593-0050, Sangon Biotech (Shanghai) Co., Ltd.).

[0089] Table 3

[0090]

[0091] The results are shown in the Experimental Results and Analysis section below.

[0092] Comparative Example 1: Effect of tyrosine on the absorption of pesticide thifluzamide in rice

[0093] The difference from Example 1 is that the organic acid used is tyrosine, and the concentration of tyrosine used is 0-0.1 mM.

[0094] Comparative Example 2: Effect of phenylalanine on the absorption of pesticide thifluzamide in rice

[0095] The difference from Example 1 is that the organic acid used is phenylalanine, and the concentration of phenylalanine used is 0-0.1 mM.

[0096] Comparative Example 3: Effect of Citric Acid on the Absorption of Pesticide Tebuconazole in Rice

[0097] The difference from Example 1 is that the organic acid used is citric acid, the concentration of citric acid used is 0.1 mM, the pesticide used is tebuconazole, and the concentration of tebuconazole used is 20 mg / kg.

[0098] Experimental Results and Analysis:

[0099] 1. The results of Example 1, Comparative Example 1, and Comparative Example 2 are as follows: Figure 1 As shown.

[0100] The results showed that after treating rice roots with small-molecule organic acids for 5 days:

[0101] Tyrosine and phenylalanine did not promote the root absorption and upward transport of pesticides in rice. Adding different concentrations of tryptophan significantly increased thifluzamide accumulation in rice roots; among them, 1 mM tryptophan resulted in the highest thifluzamide accumulation in rice roots. Adding different concentrations of succinic acid also significantly increased both root accumulation and upward transport of thifluzamide in rice, with 1 mM resulting in the highest accumulation in rice roots, and 0.1 mM resulting in the highest accumulation in rice stems and leaves. Adding different concentrations of citric acid also significantly increased thifluzamide accumulation in both rice roots and stems and leaves; the highest accumulation of thifluzamide in rice roots was observed at a citric acid concentration of 0.1 mM, while the highest translocation rate of thifluzamide in rice was observed at a citric acid concentration of 0.01 mM.

[0102] 2. The results of Example 2 are as follows Figures 2-4 As shown.

[0103] The results showed that on the third day after adding succinic acid, citric acid, or tryptophan to the rice nutrient solution, the content of thifluzamide in the roots and stems of rice was significantly increased compared with the control group, indicating that succinic acid, citric acid, and tryptophan can all promote the absorption and transport of the pesticide thifluzamide in rice.

[0104] The above experimental results indicate that succinic acid, citric acid, and tryptophan can all promote the absorption and transport of the pesticide thifluzamide in rice at relatively low concentrations.

[0105] 3. The results of Comparative Example 3 are as follows: Figure 5 As shown.

[0106] The results showed that when rice seedlings were cultured in a nutrient solution containing 0.1 mM citric acid and 20 mg / kg tebuconazole for 5 and 7 days, citric acid failed to promote the absorption of tebuconazole by rice roots or its translocation within the rice plant. Furthermore, on day 7, the concentration of tebuconazole in the stems and leaves of the citric acid group was significantly lower than that in the control group (CK), indicating that citric acid inhibited the translocation of tebuconazole. These data suggest that the promoting effect of citric acid on the absorption of different pesticides in rice varies significantly, and may even be contradictory. Therefore, selecting appropriate pesticides is more conducive to the effective role of citric acid in promoting pesticide absorption and utilization in rice.

[0107] 4. The results of Example 3 are as follows Figure 6 As shown.

[0108] Malondialdehyde (MDA) is an important product of plant membrane lipid peroxidation and can indirectly reflect the degree of peroxidative damage to plant membrane systems. It is often used to evaluate the extent of damage suffered by plants under stress from exogenous pollutants. Figure 6As shown, 0.1 mM succinic acid and 1 mM tryptophan can significantly reduce the malondialdehyde content in roots, indicating that succinic acid and tryptophan can reduce the damage of the pesticide thifluzamide to rice and play a role in protecting the plant.

[0109] Reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) are effective substances for scavenging reactive oxygen species in plant root cells, and their enzyme activity levels can reflect the ability of plant roots to resist pesticide oxidative stress. Figure 6 The results showed that succinic acid and citric acid could both increase the activity of catalase, while tryptophan increased the activity of superoxide dismutase, indicating that these three organic acids could enhance the ability of plant roots to resist pesticide oxidative stress.

[0110] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be considered equivalent substitutions and shall be included within the protection scope of the present invention.

Claims

1. The application of small molecule organic acids in promoting the absorption and / or transport of the pesticide thifluzamide by crops, characterized in that, The small molecule organic acid is succinic acid.

2. The application of small molecule organic acids in the preparation of products that promote the absorption and / or translocation of the pesticide thifluzamide by crops, characterized in that, The small molecule organic acid is succinic acid.

3. The application of small molecule organic acids in the preparation of synergists for the pesticide thifluzamide, characterized in that, The small molecule organic acid is succinic acid.

4. The application of small molecule organic acids in promoting the absorption and translocation of pesticides by crops and enhancing the ability of crop roots to resist pesticide oxidative stress, characterized in that... The small molecule organic acid is succinic acid, and the pesticide is thifluzamide.

5. The application of small molecule organic acids in the preparation of products that promote the absorption and translocation of pesticides by crops and enhance the ability of crop roots to resist pesticide oxidative stress, characterized in that, The small molecule organic acid is succinic acid, and the pesticide is thifluzamide.

6. A method for promoting the absorption and / or translocation of the pesticide thifluzamide in crops, characterized in that, A small molecule organic acid and thifluzamide are applied together to the roots of crops, wherein the small molecule organic acid is succinic acid.

7. The application of succinic acid in enhancing the efficacy of thifluzamide in controlling crop diseases or in the preparation of products that enhance the efficacy of thifluzamide in controlling crop diseases, characterized in that, The crop diseases mentioned include sheath blight, white mold, and stem rot.

8. The application according to claim 7, characterized in that, The concentration of succinic acid is 0.01–10 mM.

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