Guava seedling growth regulating composition and seedling raising method

Abstract

The present application belongs to the field of guava cultivation. Specifically provided is a guava seedling growth regulating composition and a seedling method, which comprises (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl)(phenyl)methanone, and the concentration is 5-20 mg / L. The growth regulating composition can promote the guava seedling to quickly root and grow leaves, adapt to the environment, and potentially improve the survival rate of planting.

Description

Technical Field

[0001] This invention relates to the field of passion fruit cultivation technology, specifically to a passion fruit seedling growth regulation composition and seedling cultivation method. Background Technology

[0002] Passion fruit (scientific name: *Passiflora edulia* Sims.) is a perennial climbing vine belonging to the genus *Passiflora* in the family Passifloraceae. It has fleshy, shallow roots, a hollow, smooth, hairless stem, and tendrils for climbing. The leaves are alternate, three-lobed, and green. There are five petals, as long as the sepals. The berry is ovoid, 3-4 cm in diameter, and hairless. When ripe, it is purple, yellow, or purplish-red. Native to South America, it is widely cultivated in tropical and subtropical regions. Passion fruit is named for its various fruity aromas; it is sweet and sour, with a rich flavor, earning it the reputation of "King of Juices." It contains 17 essential amino acids, various vitamins, carotenoids, and trace elements, making it highly nutritious. Passion fruit can be processed into juice, syrup, jam, and jelly; the peel can be used as animal feed and for pectin extraction; the roots, stems, and leaves can all be used medicinally, possessing effects such as clearing the lungs and moisturizing dryness, calming the nerves and relieving pain, promoting blood circulation and strengthening the body, nourishing yin and tonifying the kidneys, and lowering blood lipids and blood pressure. Summary of the Invention

[0003] This invention provides a passion fruit seedling growth regulator composition comprising (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-7-yl)(phenyl)methyl ketone, at a concentration of 5-20 mg / L. This growth regulator composition promotes rapid root and leaf growth in passion fruit seedlings, facilitating environmental adaptation and potentially increasing the survival rate after transplanting.

[0004] Specifically, the present invention provides a passion fruit seedling growth regulating composition comprising (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-7-yl)(phenyl)methyl ketone, wherein the concentration is 5~20 mg / L, preferably 10 mg / L.

[0005] In some embodiments, the passion fruit seedling growth regulating composition further includes (2,4-dichlorophenyl)(3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-7-yl) methyl ketone, with a concentration of 5-20 mg / L; preferably 10 mg / L.

[0006] In some embodiments, the mass concentration ratio of (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl)(phenyl) methyl ketone to (2,4-dichlorophenyl)(3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl) methyl ketone is 1:(0.8~1.2).

[0007] In some embodiments, the passion fruit seedling growth regulating composition further includes potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate.

[0008] In some embodiments, potassium nitrate is 1200~1500 mg / L, and the mass ratio of other components to potassium nitrate is: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1: (1~1.5): (0.8~1.2): (0.3~0.6): (0.2~0.5): (0.2~0.5).

[0009] In some embodiments, potassium nitrate is 1200 mg / L, and the mass ratio of other components to potassium nitrate is: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3.

[0010] On the other hand, the present invention provides a method for cultivating passion fruit seeds, the method comprising the following steps: 1) Soaking seeds; 2) Germination; 3) Seedling raising; During the seedling cultivation process, the seedlings are sprayed with a nutrient solution containing the passion fruit seedling growth regulating composition described in this invention every 5 days.

[0011] In some embodiments, the method includes the steps of: 1) Soaking seeds: Soak passion fruit seeds in water with pH 8 for 2-3 hours. Then wrap 80-120 grams of seeds in 80-mesh gauze, tie it with a string, and put it in water at 60-70°C for 1-3 seconds. Let the surface moisture dry naturally. Repeat this process 4-5 times. Then soak the seeds in clean water with trisodium phosphate at 20-30°C for 8-10 hours. 2) Germination: Germinate the soaked passion fruit seeds in a 32℃ germination chamber. Stop germination when 30% of the seeds have sprouted. 3) Seedling raising: Take several seedling cups with a diameter of 25-30cm and a depth of 30-35cm. Put 2-3 pre-germinated passion fruit seeds into each seedling cup, add the nutrient solution of the passion fruit seedling growth regulating composition described in this invention, and raise the seedlings in an environment with a room temperature of 27-32℃ and a relative humidity of 50-70%. Spray the seedlings with nutrient solution every 5 days. When the seedlings grow to a stem diameter of 0.5cm and a height of 40-50cm, they can be transplanted or used as rootstock for grafting.

[0012] On the other hand, the present invention also provides a method for passion fruit propagation by cuttings, the method comprising the following steps: a) Insert passion fruit 6-10 cm branches into the seedbed, spray with water to keep it moist, and wait for it to take root; b) Apply the nutrient solution of the passion fruit seedling growth regulating composition of the present invention to the branch segments that have grown 4-5 roots.

[0013] In some embodiments, the method includes the steps of: a) Lay a clean plastic film on a flat surface in the greenhouse. Take river sand, wash off the soil, and immerse it in boiling water for 5 minutes. After taking it out, cool it to about 50 ℃ and spread it evenly on the plastic film to form a seedbed 6-8 cm thick. Cut the branches into sections and insert them into the seedbed with a spacing of 4 cm × 8 cm. Spray clean water 2-3 times a day to keep the seedbed moist until it takes root. b) When the branch cuttings have grown 4-5 strong roots, transplant them into nutrient bags filled with garden soil. After 25 days, they can be planted in the orchard with soil. Alternatively, when the branch cuttings have grown 4-5 strong roots, continue to grow them in the seedbed, spraying them with nutrient solution every 3 days. After 20 days, transplant them into the orchard and manage them according to conventional methods. The nutrient bag is made by mixing 40% coconut coir, 30% peat moss, 20% vermiculite, and 10% perlite by weight percentage, and then soaking it in a nutrient solution. The nutrient solution is an aqueous solution containing the passion fruit seedling growth regulating composition described in this invention.

[0014] Beneficial effects The passion fruit seedling growth regulating composition provided by this invention can promote rapid root and leaf growth during passion fruit seedling cultivation. In seed cultivation, it can increase stem diameter and stem height by more than 24% and 34% respectively within 60 days. The rapid growth of seedlings can enhance their ability to adapt to the environment and potentially improve the survival rate after transplanting.

[0015] Terminology Explanation Certain embodiments of the present invention will now be described in detail. The present invention is intended to cover all alternatives, modifications, and equivalents, all of which are included within the scope of the invention as defined in the claims. Those skilled in the art will recognize that many similar or equivalent methods and materials described herein can be used to practice the present invention. The present invention is by no means limited to the methods and materials described herein. In the event that one or more of the incorporated documents, patents, and similar materials differ from or contradict this application (including, but not limited to, defined terminology, application of terminology, described techniques, etc.), this application shall prevail.

[0016] It should be further appreciated that certain features of the invention, for clarity, have been described in multiple independent embodiments, but may also be provided in combination in a single embodiment. Conversely, various features of the invention, for brevity, have been described in a single embodiment, but may also be provided individually or in any suitable sub-combination.

[0017] Unless otherwise stated, all technical terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art. All patents and publications related to this invention are incorporated herein by reference in their entirety.

[0018] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0019] In the following content, all numbers disclosed herein, whether or not they use words such as "approximately" or "about," are approximate values. The value of each number may vary by 1%, 2%, 5%, 7%, 8%, 10%, 15%, or 20%. Whenever a number with a value of N is disclosed, any numbers with values ​​of N+ / -1%, N+ / -2%, N+ / -3%, N+ / -5%, N+ / -7%, N+ / -8%, N+ / -10%, N+ / -15%, or N+ / -20% will be explicitly disclosed, where "+ / -" indicates addition or subtraction. Detailed Implementation

[0020] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. The specific embodiments described herein are for illustrative purposes only and are not intended to limit the invention in any way. Furthermore, descriptions of well-known structures and techniques are omitted in the following description to avoid unnecessarily obscuring the concepts of this disclosure. Such structures and techniques have also been described in many publications.

[0021] All reagents used in this invention can be purchased commercially or prepared by methods disclosed in the prior art or by the methods described in this invention.

[0022] Synthesis of accelerators:

[0023] Step 1, Synthesis of compound (ii) The mixture of acetic acid (i) and thiocarboxyhydrazide was refluxed under stirring for 4 hours. After concentration under reduced pressure, the crude product was recrystallized from ethanol to give compound (ii).

[0024] Step 2, Synthesis of compound (iii) To a solution of compound (ii) (2 mmol) in anhydrous alcohol (20 mL), substituted benzaldehyde (2 mmol) was added, and the pH was adjusted to 5–6 with dilute hydrochloric acid. The mixture was refluxed for approximately 4 hours. The solid obtained after filtration and cooling was washed with cold water, dried, and recrystallized from ethanol to give compound (iii).

[0025] Step 3: Synthesis of compound IV Compound (iii) (2.2 mmol) and the substituted 2-bromo-1-phenylethyl-1-one (2 mmol) were reacted in a mixture of ethanol (40 mL) and triethylamine (1 mL) at room temperature for about 30 min to give compound iv.

[0026] When the raw material in step 2 replaces benzaldehyde with benzaldehyde, and the 2-bromo-1-phenylethyl-1-one substituted in step 3 is 2-bromo-1-(2,4-dichlorophenyl)ethyl-1-one, then compound iv is (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl)(phenyl)methyl ketone, hereinafter referred to as compound 1.

[0027] Compound 1 1 H NMR (500MHz, DMSO- d 6) d : 2.34 (s, 3H), 5.05 (t,J = 5.8Hz, 1H), 5.76(d, J = 5.2Hz, 1H,), 7.04 (d, J = 6.7Hz, 1H), 7.28 (t, J = 7.2Hz, 1H), 7.34 (t, J = 7.6Hz, 2H), 7.50 (d, J = 7.6Hz, 2H,), 7.58 (t, J = 7.8Hz, 2H), 7.71 (t, J = 7.4Hz, 1H), 8.06 (d, J = 7.5 Hz, 2H).

[0028] When the raw material in step 2 replaces benzaldehyde with benzaldehyde, and the 2-bromo-1-phenylethyl-1-one substituted in step 3 is 2-bromo-1-phenylethyl-1-one, compound iv is (2,4-dichlorophenyl)(3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl) methyl ketone, hereinafter referred to as compound 2.

[0029] Compound 2 1 H NMR (500MHz, DMSO- d 6) d : 2.35 (s, 3H), 5.03 (t, J = 6.2Hz, 1H), 5.53(d, J = 5.3Hz, 1H), 7.06 (d, J = 7Hz, 1H), 7.28(t, J = 7Hz, 1H), 7.34 (t, J =6.9Hz, 2H), 7.43 (d, J = 7.4Hz, 2H), 7.54 (dd, J 1 = 1.8Hz J 2 = 8.4Hz, 1H), 7.63(d, J = 8.4Hz, 1H), 7.76 (d, J = 1.7Hz, 1H).

[0030] Example 1: Seedling cultivation Soaking: Soak passion fruit seeds in cold water at pH 8 (room temperature water is fine) for 2-3 hours. Then wrap 80-120 grams of seeds in 80-mesh gauze, tie it with a string, and put it in water at 60-70°C for 1-3 seconds. Let the surface moisture dry naturally. Repeat this process 4-5 times. Then soak the seeds in clean water at 20-30°C with trisodium phosphate for 8-10 hours.

[0031] Germination: Germinate the soaked passion fruit seeds in a 32℃ germination chamber. Stop germination when 30% of the seeds have sprouted.

[0032] Seedling raising: Take multiple seedling cups with a diameter of 25-30cm and a depth of 30-35cm, put 2-3 pre-germinated passion fruit seeds into each seedling cup, add the nutrient solution of the passion fruit seedling growth regulating composition described in this invention, and raise the seedlings in an environment with a room temperature of 27-32℃ and a relative humidity of 50-70%; spray with nutrient water every 5 days; when the seedlings grow to a stem diameter of 0.5cm and a height of 40-50cm, they can be transplanted or used as rootstock for grafting.

[0033] The seedlings were grouped based on different nutrient solutions, with 10 seedling cups set up in each group, and the growth status of passion fruit seedlings in each group was recorded.

[0034] For seedling group 1, the nutrient solution was selected to consist of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3; the solution was water.

[0035] For seedling group 2-1, the nutrient solution was selected to consist of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 1 was added to bring the concentration to 10 mg / L. The solution was water.

[0036] Seedling group 2-2 uses a nutrient solution composed of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate. The potassium nitrate concentration is 1200 mg / L. The mass ratio of the other components to potassium nitrate is: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 1 is also added to bring the concentration to 20 mg / L. The solution is water.

[0037] For seedling groups 2-3, the nutrient solution consisted of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at a concentration of 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 1 was also added, bringing the concentration to 30 mg / L. The solution was water.

[0038] For seedling group 3-1, the nutrient solution was selected to consist of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 2 was added to bring the concentration to 10 mg / L. The solution was water.

[0039] For seedling group 3-2, the nutrient solution was composed of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 2 was added to bring the concentration to 20 mg / L. The solution was water.

[0040] For seedling group 3-3, the nutrient solution was selected to consist of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 2 was added to bring the concentration to 30 mg / L. The solution was water.

[0041] For seedling group 4-1, the nutrient solution consisted of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 1 and compound 2 were added, with a final concentration of 5 mg / L each. The solution was water.

[0042] For seedling group 4-2, the nutrient solution consisted of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 1 and compound 2 were added, with a final concentration of 10 mg / L each. The solution was water.

[0043] For seedling group 4-3, the nutrient solution consisted of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at 1200 mg / L. The mass ratio of the other components to potassium nitrate was: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 1 and compound 2 were added, with a final concentration of 20 mg / L each. The solution was water.

[0044] The growth status of passion fruit seedlings is recorded in Table 1.

[0045] Table 1

[0046] Note the measurement method: Stem diameter: Measured with vernier calipers (accuracy 0.02 mm). For seedlings, the maximum value of the newly grown stem is used.

[0047] Stem height: Measured with a ruler (1mm accuracy). Starting from the substrate leveling line, measure the highest point of the plant.

[0048] Example 2: Propagation by Cuttings Take disease-free, semi-lignified stem segments from the current year's growth of the golden fruit and cut them into 6-10 cm sections, each section containing 2 nodes. Remove the leaves from the basal node, leaving one leaf on the upper node. Bundle 30-50 sections together, soak the cuttings in a special disinfectant solution, and then propagate them in river sand substrate.

[0049] Lay a clean plastic film on a flat surface in the greenhouse. Wash river sand to remove the soil and immerse it in boiling water for 5 minutes. After removing it, cool it to about 50°C and spread it evenly on the plastic film to form a seedbed 6-8 cm thick. Insert the branch cuttings into the seedbed, with a spacing of 4 cm × 8 cm. Spray clean water 2-3 times a day to keep the seedbed moist. When the branch cuttings have grown 4-5 strong roots, transplant them into nutrient bags filled with garden soil. After 25 days, they can be planted in the orchard with soil. Alternatively, they can continue to grow in the seedbed, spraying nutrient solution every 3 days. After 20 days, they can be transplanted to the orchard and managed according to conventional methods.

[0050] The nutrient bag is made by mixing 40 wt% coconut coir, 30 wt% peat moss, 20 wt% vermiculite, and 10 wt% perlite by weight percentage, and then soaking it in a nutrient solution. The nutrient solution consists of potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate, with potassium nitrate at a concentration of 1200 mg / L. The ratio of the other components to potassium nitrate is: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.3. Compound 1 and Compound 2 are added, with a final concentration of 10 mg / L each.

[0051] The group without the addition of compounds 1 and 2 was designated as the cutting propagation group 1; the group with the addition of compounds 1 and 2 was designated as the cutting propagation group 2.

[0052] The growth status of passion fruit seedlings is recorded in Table 2.

[0053] Table 2

[0054] Note the measurement method: Stem diameter: Measured with vernier calipers (accuracy 0.02mm). For cutting propagation, the minimum measured value of the cutting stem is used.

[0055] Stem height: Measured with a ruler (1mm accuracy). Starting from the substrate leveling line, measure the highest point of the plant.

[0056] The method of this invention has been described through preferred embodiments. Those skilled in the art will readily be able to modify or appropriately alter and combine the methods and applications described herein within the scope, spirit, and context of this invention to implement and apply the technology of this invention. Those skilled in the art can refer to the content herein to appropriately improve process parameters. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art and are considered to be included within the scope of this invention.

Claims

1. A passion fruit seedling growth regulator composition, characterized in that, Including (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl)(phenyl) methyl ketone and (2,4-dichlorophenyl)(3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl) methyl ketone, wherein (3-methyl-6... The concentration of phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl)(phenyl) methyl ketone is 5~20 mg / L, and the concentration of (2,4-dichlorophenyl)(3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl) methyl ketone is 5~20 mg / L.

2. The passion fruit seedling growth regulating composition according to claim 1, characterized in that, The concentration of (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazine-7-yl)(phenyl)methyl ketone was 10 mg / L.

3. The passion fruit seedling growth regulating composition according to claim 1, characterized in that, The concentration of (2,4-dichlorophenyl)(3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl) methyl ketone was 10 mg / L.

4. The passion fruit seedling growth regulating composition according to claim 1, characterized in that, The mass concentration ratio of (3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl)(phenyl) methyl ketone to (2,4-dichlorophenyl)(3-methyl-6-phenyl-6,7-dihydro-5H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazin-7-yl) methyl ketone is 1:(0.8~1.2).

5. The passion fruit seedling growth regulating composition according to any one of claims 1-4, characterized in that, It also includes potassium nitrate, magnesium sulfate, ammonium phosphate, potassium iodide, zinc sulfate, and calcium dihydrogen phosphate.

6. The passion fruit seedling growth regulating composition according to claim 5, characterized in that, The potassium nitrate concentration is 1200~1500 mg / L. The mass ratio of other components to potassium nitrate is as follows: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1: (1~1.5): (0.8~1.2): (0.3~0.6): (0.2~0.5): (0.2~0.5).

7. The passion fruit seedling growth regulating composition according to claim 5, characterized in that, The potassium nitrate content is 1200 mg / L. The mass ratio of the other components to the potassium nitrate is: potassium nitrate: magnesium sulfate: ammonium phosphate: potassium iodide: zinc sulfate: calcium dihydrogen phosphate = 1:1.2:1:0.5:0.3:0.

3.

8. A method for raising passion fruit seedlings, characterized in that, Including the following steps: 1) Soaking seeds; 2) Germination; 3) Seedling raising; During the seedling raising process, the seedlings are sprayed with a nutrient solution of the passion fruit seedling growth regulating composition according to any one of claims 1-7 every 5 days.

9. The method according to claim 8, characterized in that, Including the following steps: 1) Soaking seeds: Soak passion fruit seeds in water with pH 8 for 2-3 hours. Then wrap 80-120 grams of seeds in 80-mesh gauze, tie it with a string, and put it in water at 60-70°C for 1-3 seconds. Let the surface moisture dry naturally. Repeat this process 4-5 times. Then soak the seeds in clean water with trisodium phosphate at 20-30°C for 8-10 hours. 2) Germination: Germinate the soaked passion fruit seeds in a 32℃ germination chamber. Stop germination when 30% of the seeds have sprouted. 3) Seedling raising: Take multiple seedling cups with a diameter of 25-30cm and a depth of 30-35cm, put 2-3 pre-germinated passion fruit seeds into each seedling cup, add a nutrient solution of the passion fruit seedling growth regulating composition according to any one of claims 1-7, and raise the seedlings in an environment with a room temperature of 27-32℃ and a relative humidity of 50-70%; spray with nutrient water every 5 days; when the seedlings grow to a stem diameter of 0.5cm and a height of 40-50cm, they can be transplanted or used as rootstock for grafting.

10. A method for propagating passion fruit by cuttings, characterized in that, Including the following steps: a) Insert passion fruit 6-10 cm branches into the seedbed, spray with water to keep it moist, and wait for it to take root; b) Apply an aqueous nutrient solution containing the passion fruit seedling growth regulator composition according to any one of claims 1-7 to the branch segments that have grown 4-5 roots.

11. The method according to claim 10, characterized in that, Including the following steps: a) Lay a clean plastic film on a flat surface in the greenhouse. Take river sand, wash off the soil, and immerse it in boiling water for 5 minutes. After taking it out, cool it to 50 ℃ and spread it evenly on the plastic film to form a seedbed 6-8 cm thick. Cut the branches into sections and insert them into the seedbed with a spacing of 4 cm × 8 cm. Spray clean water 2-3 times a day to keep the seedbed moist until it takes root. b) When the branch cuttings have grown 4-5 strong roots, transplant them into nutrient bags filled with garden soil. After 25 days, they can be planted in the orchard with soil attached. Alternatively, when the branch cuttings have grown 4-5 strong roots, continue to grow them in the seedbed, spraying them with nutrient solution every 3 days. After 20 days, transplant them into the orchard and manage them according to conventional methods. The nutrient bag is made by mixing 40 wt% coconut coir, 30 wt% peat moss, 20 wt% vermiculite, and 10 wt% perlite by weight percentage, and then soaking it in a nutrient solution; the nutrient solution is a nutrient aqueous solution of the passion fruit seedling growth regulating composition according to any one of claims 1-7.

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