3D CFC / GWs / Au电极结合DNAzyme逻辑门控生物传感平台及其应用
By combining 3D CFC/GWs/Au electrodes with a DNAzyme logic-gated biosensor platform, the problem of insufficient delivery platform for DNA biocomputation in biosensors was solved, achieving high sensitivity and specificity detection of exosomal microRNA in non-small cell lung cancer and simplifying the detection process.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
- Filing Date
- 2024-01-29
- Publication Date
- 2026-07-17
AI Technical Summary
When existing DNA biocomputing is applied in biosensors, there are problems such as insufficient delivery platforms, low delivery concentration, and easy degradation in complex biological environments. Furthermore, building a highly integrated DNA biocomputing platform with multiple miRNAs as input signals for logical function activation remains a challenge.
A 3D CFC/GWs/Au electrode combined with a DNAzyme logic-gated biosensing platform is adopted, including an electrochemical detection device, a DNA tetrahedral structure, a Zr-MOF-Au-Fc-H1 beacon probe, and a logic gate module. Cu2+ is used to activate the DNAzyme to cleave the substrate chain, thereby realizing the logic gate output of various miRNAs.
It achieves high sensitivity and specificity in the detection of exosomal microRNAs in non-small cell lung cancer, simplifies the clinical personalized testing and diagnostic analysis process, and can simultaneously detect multiple miRNAs and perform logical interpretation.
Smart Images
Figure CN117929498B_ABST