A reagent sample injection distribution control system based on a double microfluidic chip clamp and a use method thereof

Abstract

The application provides a reagent sample injection distribution control system based on a double micro-fluidic chip clamp and a use method, which comprises a reagent sample injection distribution control module for reagent sample injection distribution; the reagent sample injection distribution control module is connected with a double micro-fluidic chip clamp in cooperation; the reagent sample injection distribution control module is connected with a liquid crystal screen control module for controlling a sample injection process at the same time; the system can realize the distribution and automatic control of multiple reagents at the same time, improves the work efficiency in the medical and biological fields, and has a simple structure, convenient operation, practical significance and good application prospect.

Description

Technical Field

[0001] This invention relates to the field of microfluidic chip technology, and in particular to a reagent injection and dispensing control system and its usage method based on a dual microfluidic chip fixture. Background Technology

[0002] Microfluidic chip technology is a scientific technology characterized by manipulating fluids in a micrometer-scale space. It integrates various basic operations onto a chip of just a few square centimeters, such as cell culture, sorting, lysis, sample preparation, reaction, separation, and detection. Due to its safety, low cost, high integration, and low pollution, microfluidic chip technology is widely used in microelectronics, biomedical engineering, fluid physics, and chemical engineering, especially in biomedical research where it has enormous development potential and broad application prospects. Fluid sample introduction is a crucial step in microfluidic chip technology; however, most current fluid sample introduction platforms have complex structures and low integration, only capable of single reagent introduction and limited to sample introduction functions. They are difficult to integrate, and achieving multiple functions requires multiple chip fixtures, hindering portability and increasing costs.

[0003] For example, patent number CN202210254661 discloses a flexible microfluidic chip fixture and a flexible microfluidic chip fixture assembly. The fixture includes a first clamping plate, a chip adapter, a second clamping plate, a flexible connector, and a locking structure. This fixture uses a flexible microfluidic chip, which effectively prevents the fragile chip material from directly contacting the fixture. However, the flexible microfluidic chip also increases the cost, requires advanced manufacturing processes, and is difficult to integrate and port.

[0004] Currently, there is no sample introduction platform that can simultaneously control and switch between different reagents in multiple microfluidic chips for sample introduction and dispensing. Summary of the Invention

[0005] To address the shortcomings of the existing technology, this invention provides a reagent injection and dispensing control system and its usage method based on a dual microfluidic chip fixture. This system can simultaneously dispense and automatically control multiple reagents, improving work efficiency in the medical and biological fields. Moreover, this system has a simple structure, is easy to operate, and has practical significance and good application prospects.

[0006] To achieve the above-mentioned technical features, the objective of this invention is as follows: a reagent injection and dispensing control system based on a dual microfluidic chip fixture, comprising a reagent injection and dispensing control module for reagent injection and dispensing; the reagent injection and dispensing control module is connected in cooperation with the dual microfluidic chip fixture; the reagent injection and dispensing control module is also connected to an LCD screen control module for controlling the sample injection process. The dual microfluidic chip fixture includes a sample dispensing control chip stage, a fixture cover plate installed on the top of the sample dispensing control chip stage via a sliding groove, an injection pressure plate installed inside the fixture cover plate, a replaceable sample dispensing control chip disposed between the injection pressure plate and the sample dispensing control chip stage, and capable of dispensing and controlling different types of reagents, a fixture base installed on the bottom of the sample dispensing control chip stage via a sliding groove, and a microfluidic detection chip stage disposed between the sample dispensing control chip stage and the fixture base; The reagent injection and dispensing control module includes a positive pressure injection device for achieving positive pressure injection and a negative pressure injection device for achieving negative pressure injection. The LCD screen control module includes a circuit board, which is connected to the display screen.

[0007] Preferably, the positive pressure injection device includes a plunger pump, a flow guide plate, a solenoid valve, and reagent tubes. The solenoid valves are arranged and installed on the flow guide plate. The air inlet of the flow guide plate is connected to the plunger pump. The air outlet of the flow guide plate is connected to the air inlet pipe. The reagent tubes are arranged and connected to the sample outlet pipe and the air inlet pipe respectively. The other end of the sample outlet pipe is connected to the dual microfluidic chip fixture. The negative pressure injection device includes a pressure regulating valve, a dispensing bottle, and a peristaltic pump. The sample outlet of the dual microfluidic chip fixture is connected to the dispensing bottle through a sample outlet tube. The other end of the dispensing bottle is connected to the pressure regulating valve through a plastic tube, and the other side of the pressure regulating valve is connected to the peristaltic pump through a plastic tube.

[0008] Preferably, the dual microfluidic chip fixture enables the use of two types of microfluidic chips in combination. The sample dispensing control chip and the microfluidic detection chip are connected in a sealed manner through the sample dispensing control chip stage, so as to realize two control modes: sample dispensing control and automated control of the experimental process. The control system can simultaneously perform precise injection control of 6 reagents, and can also push and extract reagents through a positive pressure injection device; The replaceable sample dispensing control chip in the dual microfluidic chip fixture can be selected from different types of chips.

[0009] Preferably, one end of the air inlet tube and the sample outlet tube connected to the reagent tube is connected to a metal needle, and the metal needle of the air inlet tube is located at the rubber part of the reagent tube opening, while the metal needle of the sample outlet tube is located at the bottom of the reagent tube.

[0010] Preferably, a lower deep groove is formed in the middle of the fixture base, and the microfluidic detection chip stage is supported inside the lower deep groove. The microfluidic detection chip stage is pressed and sealed with the sample dispensing control chip stage by the lower spring of the fixture base. The sample dispensing control chip stage is fixed to the lower groove on the fixture base by the lower insert plate, and the sample dispensing control chip stage is fixed to the upper cover plate of the fixture by the upper insert plate. An upper deep groove is formed inside the upper cover plate of the fixture, and the sample injection pressure plate is connected to the upper spring, pressing the sample injection pressure plate on the top of the sample dispensing control chip stage.

[0011] Preferably, the fixture base has symmetrically machined strip-shaped downward grooves on both sides, which are used to fit with the sample dispensing control chip stage for insertion; a square hole is provided in the lower deep groove of the fixture base, which is adapted to the lower boss of the microfluidic detection chip stage; The fixture base and the microfluidic detection chip stage are respectively provided with evenly distributed and corresponding lower spring holes on their mating surfaces. The fixture base and the microfluidic detection chip stage are connected by the lower springs. When the microfluidic detection chip stage is pressed to compress the lower springs, it will move downwards. The fixture base is symmetrically machined with first square grooves in the middle of the front and back. The first square grooves are used for sliding limit of the microfluidic detection chip stage. The first square grooves are provided with first ear plates on both sides of the outer wall of the fixture base. The first ear plates on both sides are respectively machined with first through holes of different diameters in the middle. The first through holes are used to pass through the first pins and fix the microfluidic detection chip stage to the fixture base.

[0012] Preferably, the microfluidic detection chip stage has first ear seats on both the front and rear sides for cooperating with the first square groove. The first ear seats are machined with first pin holes for passing through the first pin. The bottom of the microfluidic detection chip stage has a lower boss in the middle. The bottom of the lower boss has a sample outlet hole that passes through the lower boss and the microfluidic detection chip stage. The sample outlet hole at the bottom of the lower boss is fitted with a first fixing bolt. The top of the microfluidic detection chip stage has an opening for a first vacuum suction cup. The first vacuum suction cup and the first fixing bolt are connected by a plastic tube.

[0013] Preferably, the sample dispensing control chip stage has a groove in the middle, and a hole through the sample dispensing control chip stage is formed in the middle of the groove. A second vacuum suction cup is provided above and below the hole, and the second vacuum suction cups are connected by a plastic tube. An upper and lower insert plate are symmetrically arranged on the upper and lower sides of the sample dispensing control chip stage, and the upper and lower insert plates are respectively used for insertion and fixing with the upper cover plate of the fixture and the base of the fixture. A rectangular groove is provided in the center of the front and rear of the sample dispensing control chip stage for limiting the movement of the sample injection plate.

[0014] Preferably, the upper cover plate of the fixture is provided with an upper deep groove, and square openings are provided on the upper left and right sides of the upper deep groove. The square openings are adapted to the upper protrusions on the sample injection plate. The upper cover plate of the fixture is symmetrically machined with upper sliding grooves on both sides. The upper sliding grooves are used to fix the sample injection control chip stage. The upper deep groove of the fixture and the upper surface of the sample injection plate are also provided with evenly distributed and corresponding upper spring holes. The upper cover plate of the fixture and the sample injection plate are connected by upper springs. Pressing the microfluidic detection chip stage will move downwards. The upper cover plate of the fixture is symmetrically machined with second square grooves in the middle of the front and rear. The second square grooves are used to slide and limit the sample injection plate. Second ear plates are provided on both sides of the bottom of the front and rear second square grooves. Second through holes with different diameters are machined in the middle of the second ear plates on both sides. The second through holes are used to pass through the second pins and fix the sample injection plate to the upper cover plate of the fixture. The sample injection plate has second lugs of the same specifications on both the front and rear sides. The second lugs are symmetrically machined with second pin holes on both sides, and the second pin holes are used to pass through the second pins. The sample injection plate has upper bosses of the same specifications on the left and right sides above it. The upper bosses have three evenly distributed sample injection holes, which pass through the upper bosses and the sample injection plate. A second fixing bolt is installed at the sample injection hole at the top of the upper bosses. A third vacuum suction cup is installed at the sample injection hole at the bottom of the sample injection plate. The third vacuum suction cup is connected to the second fixing bolt through a plastic tube.

[0015] A method for using a reagent dispensing control system based on a dual microfluidic chip fixture includes the following steps: Step 1: Press down the first lugs on both sides of the microfluidic detection chip stage. The microfluidic detection chip stage will press the lower spring tightly. After the microfluidic detection chip stage reaches the bottom, insert the two first pins into the first through holes on both sides of the fixture base to fix the microfluidic detection chip stage. Then, place the microfluidic detection chip into the chip slot formed by the microfluidic detection chip stage and the fixture base. The chip slot acts as a limit. At this time, the microfluidic detection chip can only move up and down and cannot move in all directions. Step 2: Fix the sample dispensing control chip stage to the lower slot of the fixture base via the lower insert plate. Then, remove the first pins on both sides of the fixture base. At this time, the spring force of the lower spring generates an upward force on the microfluidic detection chip stage. Under this force, the microfluidic detection chip stage presses the microfluidic detection chip, and the three are sealed together by the first vacuum suction cup. Then, place the sample dispensing control chip into the dispensing control chip slot according to the corresponding sample outlet. The dispensing control chip slot acts as a limit. At this time, the sample dispensing control chip can only move up and down and cannot move in all directions. Step 3: Press the second lugs on both sides of the injection plate upwards. The injection plate will press the upper spring tightly. After the injection plate reaches the top, insert the two second pins into the second through holes of the fixture cover plate to fix the injection plate. Then, fix the dispensing control chip stage to the upper sliding groove of the fixture cover plate through the upper insert plate. Then, remove the second pins on both sides of the fixture cover plate. At this time, the elastic force of the upper spring will generate a downward force on the injection plate. Under this force, the injection plate will press the injection dispensing control chip tightly, and the three parts will be sealed together by the third vacuum suction cup. Step 4: Turn on the power and press the reagent injection volume and injection sequence on the display screen according to the experimental requirements.

[0016] The present invention has the following beneficial effects: 1. The system of this invention is equipped with two microfluidic chips with different functions, which effectively integrates the functions of reagent injection and dispensing control and experimental detection process, which is conducive to the development of automated and portable equipment.

[0017] 2. The system of the present invention has a simple structure, small size, and small footprint, making it suitable for large-scale application in biological and medical institutions.

[0018] 3. The present invention has a high degree of system integration. During use, users only need to press the corresponding button on the LCD screen according to the experimental requirements to achieve accurate sample injection of the corresponding reagents. The manual operation is minimal, so the experimental results are accurate and reliable with less external interference.

[0019] 4. The system of the present invention can replace different chips according to experimental requirements to achieve different types of reagent dispensing switching and automated control. Attached Figure Description

[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] Figure 1 This is a first-view three-dimensional diagram of the overall structure of the present invention.

[0022] Figure 2 This is a second-view three-dimensional diagram of the overall structure of the present invention.

[0023] Figure 3 This is a third-person 3D view of the overall structure of the present invention.

[0024] Figure 4 This is a first-view 3D view of the overall structure of the present invention after removing the outer shell.

[0025] Figure 5 This is a second-view 3D diagram of the overall structure of the present invention after removing the outer shell.

[0026] Figure 6This is a third-person 3D view of the overall structure of the present invention after removing the outer shell.

[0027] Figure 7 This is a top-exploded view of the present invention after the outer casing has been removed.

[0028] Figure 8 This is an exploded bottom view of the present invention after the outer casing has been removed. Detailed Implementation

[0029] The embodiments of the present invention will be further described below with reference to the accompanying drawings.

[0030] Example 1: See Figure 1-8 A reagent injection and dispensing control system based on a dual microfluidic chip fixture includes a reagent injection and dispensing control module for reagent injection and dispensing; the reagent injection and dispensing control module is connected to the dual microfluidic chip fixture 20; the reagent injection and dispensing control module is also connected to an LCD screen control module for controlling the sample injection process; the dual microfluidic chip fixture 20 includes an injection dispensing control chip stage 4, the top of the injection dispensing control chip stage 4 is fitted with a fixture upper cover plate 9 through a sliding groove, and an injection pressure plate 11 is installed inside the fixture upper cover plate 9. A replaceable sample dispensing control chip 26 is disposed between the sample dispensing control chip stage 4 and the sample dispensing control chip stage 4, enabling the dispensing and control of different types of reagents. A fixture base 1 is mounted on the bottom of the sample dispensing control chip stage 4 via a sliding groove. A microfluidic detection chip stage 2 is disposed between the sample dispensing control chip stage 4 and the fixture base 1. The reagent sample dispensing control module includes a positive pressure injection device for positive pressure injection and a negative pressure injection device for negative pressure injection. The LCD screen control module includes a circuit board 27, which is connected to a display screen 28. By employing the above-mentioned reagent sample dispensing control system, six types of reagents can be accurately injected, and different reagents can be injected according to the requirements of fluorescence in situ hybridization experiments via the sample dispensing control chip.

[0031] Further, see Figure 4 The positive pressure injection device includes a plunger pump 12, a flow guide plate 13, a solenoid valve 14, and reagent tubes 15. The solenoid valve 14 is arranged and installed on the flow guide plate 13. The air inlet 16 of the flow guide plate 13 is connected to the plunger pump 12, and the air outlet 17 of the flow guide plate 13 is connected to the air inlet pipe 18. The reagent tubes 15 are arranged and connected to the sample outlet pipe 19 and the air inlet pipe 18, respectively. The other end of the sample outlet pipe 19 is connected to the dual microfluidic chip fixture 20. By using a positive pressure injection device, positive pressure can be provided. During operation, the plunger pump 12 can supply pressure to the flow guide plate 13. After being split by the flow guide plate 13, the pressure is supplied to the air inlet pipe 18, and then supplied to the reagent tubes 15 by the air inlet pipe 18. The reagents are discharged through the sample outlet pipe 19 inside the reagent tubes 15.

[0032] Further, see Figure 4 The negative pressure injection device includes a pressure regulating valve 21, a dispensing bottle 22, and a peristaltic pump 23. The sample outlet 25 of the dual microfluidic chip holder 20 is connected to the dispensing bottle 22 via a sample outlet tube 24. The other end of the dispensing bottle 22 is connected to the pressure regulating valve 21 via a plastic tube 24, and the other end of the pressure regulating valve 21 is connected to the peristaltic pump 23 via a plastic tube 24. This negative pressure injection device provides negative pressure. During operation, the peristaltic pump 23 generates pressure, which is then regulated by the pressure regulating valve 21.

[0033] Furthermore, the dual microfluidic chip fixture 20 enables the combined use of two types of microfluidic chips. The sample dispensing control chip 26 and the microfluidic detection chip 52 are connected in a sealed manner via the sample dispensing control chip stage 4, allowing for simultaneous implementation of both sample dispensing control and automated experimental process control modes. This enhances the system's functionality.

[0034] Furthermore, the control system can simultaneously perform precise injection control of 6 reagents, and can push and extract reagents through a positive pressure injection device; Furthermore, the replaceable sample dispensing control chip 26 in the dual microfluidic chip fixture 20 can be selected from different types of chips. This enhances the system's adaptability.

[0035] Furthermore, one end of the air inlet tube 18 and the sample outlet tube 19 connected to the reagent tube 15 is connected to a metal needle 29, with the metal needle 29 of the air inlet tube 18 located at the rubber part of the reagent tube 15 opening, and the metal needle 29 of the sample outlet tube 19 located at the bottom of the reagent tube 15. This structure enables reagent transfer.

[0036] Furthermore, a lower deep groove 5 is formed in the middle of the fixture base 1. The lower deep groove 5 carries the microfluidic detection chip stage 2. The microfluidic detection chip stage 2 is pressed and sealed with the sample dispensing control chip stage 4 by the lower spring 3 of the fixture base 1. The sample dispensing control chip stage 4 is fixed to the lower sliding groove 7 on the fixture base 1 by the lower insert plate 6, and the sample dispensing control chip stage 4 is fixed to the upper cover plate 9 of the fixture by the upper insert plate 8. An upper deep groove 10 is formed inside the upper cover plate 9, which is connected to the sample injection pressure plate 11 by the upper spring 53, and presses the sample injection pressure plate 11 tightly on the top of the sample dispensing control chip stage 4. The fixture base 1 and the microfluidic detection chip stage 2 described above can conveniently fix the microfluidic detection chip between the microfluidic detection chip stage and the sample dispensing control chip stage.

[0037] Furthermore, the fixture base 1 has symmetrically machined strip-shaped downward grooves 7 on both sides, which are used for insertion and mating with the sample dispensing control chip stage 4; a square hole 30 is provided in the lower deep groove 5 of the fixture base 1, which is adapted to the lower protrusion 31 of the microfluidic detection chip stage 2; the mating surfaces of the fixture base 1 and the microfluidic detection chip stage 2 are respectively provided with evenly distributed and corresponding lower spring holes 32, and the fixture base 1 and the microfluidic detection chip stage 2 are connected by a lower spring 3, which can be pressed... The microfluidic detection chip stage 2 moves downward after compressing the spring 3. A first square groove 33 is symmetrically machined in the center of the front and rear of the fixture base 1. This first square groove 33 serves as a sliding limit for the microfluidic detection chip stage 2. First ear plates 34 are provided on both sides of the outer wall of the fixture base 1, and first through holes 35 of different diameters are machined in the center of each ear plate 34. These through holes 35 pass through a first pin 311, fixing the microfluidic detection chip stage 2 to the fixture base 1. The corresponding square holes are used to combine with the microfluidic detection chip bosses, and the first square groove 33 is used to fix the microfluidic detection chip stage 2 and the fixture base 1. Furthermore, the microfluidic detection chip stage 2 is fixed by pressing down and inserting the first pin 311, thus forming a microfluidic detection chip groove between the microfluidic detection chip stage 2 and the fixture base 1.

[0038] Furthermore, the microfluidic detection chip stage 2 has first ear seats 36 on its front and rear sides for cooperating with the first square groove 33. The first ear seats 36 have first pin holes for the first pin 311 to pass through. A lower boss 31 is located at the center of the bottom of the microfluidic detection chip stage 2. A sample outlet hole 25 is located at the bottom of the lower boss 31, penetrating both the lower boss 31 and the microfluidic detection chip stage 2. A first fixing bolt 37 is installed in the sample outlet hole 25 at the bottom of the lower boss 31. A first vacuum suction cup 38 is provided at the top of the microfluidic detection chip stage 2, and the first vacuum suction cup 38 is connected to the first fixing bolt 37 via a plastic tube. The microfluidic detection chip stage 2 can be fixed using the first ear seats 36.

[0039] Furthermore, the sample dispensing control chip stage 4 has a sample dispensing control chip stage groove 39 in the middle, and a hole penetrating the sample dispensing control chip stage 4 is opened in the middle of the sample dispensing control chip stage 4. Second vacuum suction cups 40 are provided above and below the hole, and the second vacuum suction cups 40 are connected by a plastic tube. An upper insertion plate 8 and a lower insertion plate 6 are symmetrically arranged on the upper and lower sides of the sample dispensing control chip stage 4, respectively used for insertion and fixing with the fixture upper cover plate 9 and the fixture base 1. A rectangular groove 41 is provided in the center of the front and rear of the sample dispensing control chip stage 4 for limiting the movement of the sample injection pressure plate 11. The sample dispensing control chip 26 and the microfluidic detection chip 52 can be connected through the sample dispensing control chip stage 4, the second vacuum suction cups 40, and the plastic tube between the second vacuum suction cups 40.

[0040] Furthermore, the upper cover plate 9 of the fixture is provided with an upper deep groove 10, and square openings 42 are provided on the left and right sides above the upper deep groove 10. The square openings 42 are adapted to the upper protrusions 43 on the sample injection pressure plate 11. The upper cover plate 9 is symmetrically machined with upper sliding grooves 44 on both sides. The upper sliding grooves 44 are used to fix with the sample injection distribution control chip stage 4. The upper cover plate 9 and the sample injection pressure plate 11 are also provided with evenly distributed and corresponding upper spring holes 45. The upper cover plate 9 and the sample injection pressure plate 11 are connected by a spring. The upper spring 53 connects to the microfluidic detection chip stage 2, which moves downward when pressed. The upper cover plate 9 of the fixture has symmetrically machined second square grooves 46 at its front and rear center. These grooves serve as sliding limits for the sample injection plate 11. Second ear plates 47 are provided on both sides of the bottom of the front and rear second square grooves 46. Second through holes 48 of different diameters are machined in the center of each ear plate 47. These through holes 48 are used to pass through the second pin 310 and fix the sample injection plate 11 to the upper cover plate 9 of the fixture. The sample injection plate 11 is fixed by pressing it upwards and inserting the second pin 310. Furthermore, the sample injection plate 11 and the upper cover plate 9 of the fixture are fixed together by a sliding groove.

[0041] Furthermore, the sample injection plate 11 has identical second lugs 49 on both its front and rear sides. The second lugs 49 have symmetrically machined second pin holes 50 on both sides, through which second pins 310 pass. The sample injection plate 11 has identical upper bosses 43 on its left and right sides, with three evenly distributed injection holes 51 on each boss. The injection holes 51 penetrate the bosses 43 and the sample injection plate 11. A second fixing bolt is installed at the injection hole 51 at the top of the bosses 43, and a third vacuum suction cup is installed at the injection hole 51 at the bottom of the sample injection plate 11. The third vacuum suction cup is connected to the second fixing bolt via a plastic tube. By pressing the sample injection plate 11 against the sample dispensing control chip stage 2, the sample injection plate 11 and the sample dispensing control chip 26 can be pressed together, and the third vacuum suction cup ensures a sealed fit between the three components.

[0042] Example 2: A method for using a reagent dispensing control system based on a dual microfluidic chip fixture includes the following steps: Step 1: Press down the first ear seats 36 on both sides of the microfluidic detection chip stage 2. The microfluidic detection chip stage 2 will press down the lower spring 3. After the microfluidic detection chip stage 2 reaches the bottom, insert the two first pins 311 into the first through holes 35 on both sides of the clamp base 1 to fix the microfluidic detection chip stage 2. Then, place the microfluidic detection chip 52 into the chip slot formed by the microfluidic detection chip stage 2 and the clamp base 1. The chip slot plays a limiting role. At this time, the microfluidic detection chip 52 can only move up and down and cannot move in all directions. Step 2: The sample dispensing control chip stage 4 is fixed to the sliding groove 7 of the fixture base 1 via the lower insert plate 6. Then, the first pins 311 on both sides of the fixture base 1 are removed. At this time, the elastic force of the lower spring 3 generates an upward force on the microfluidic detection chip stage 2. Under this force, the microfluidic detection chip stage 2 presses the microfluidic detection chip 52, and the three are sealed together by the first vacuum suction cup 38. Then, the sample dispensing control chip 26 is placed into the dispensing control chip slot 39 according to the sample outlet 25. The dispensing control chip slot 39 plays a limiting role. At this time, the sample dispensing control chip 26 can only move up and down and cannot move in all directions. Step 3: Press the second lugs 49 on both sides of the injection plate 11 upwards. The injection plate 11 presses the upper spring 53. After the injection plate 11 reaches the top, insert the two second pins 310 into the second through holes 48 of the fixture cover plate 9 to fix the injection plate 11. Then, fix the dispensing control chip stage 4 to the upper sliding groove 44 of the fixture cover plate 9 through the upper insert plate 8. Then, remove the second pins 310 on both sides of the fixture cover plate 9. At this time, the elastic force of the upper spring 53 generates a downward force on the injection plate 11. Under this force, the injection plate 11 presses the injection dispensing control chip 26 and seals the three together through the third vacuum suction cup. Step 4: Turn on the power and press the reagent injection volume and injection sequence on display screen 28 according to the experimental requirements.

Claims

1. A reagent dispensing control system based on a dual microfluidic chip fixture, characterized in that: It includes a reagent injection and dispensing control module for reagent injection and dispensing; the reagent injection and dispensing control module is connected in conjunction with a dual microfluidic chip fixture (20); the reagent injection and dispensing control module is also connected to an LCD screen control module for controlling the sample injection process; The dual microfluidic chip fixture (20) includes a sample dispensing control chip stage (4). A fixture cover plate (9) is mounted on the top of the sample dispensing control chip stage (4) via a sliding groove. An injection pressure plate (11) is installed inside the fixture cover plate (9). A replaceable sample dispensing control chip (26) is positioned between the injection pressure plate (11) and the sample dispensing control chip stage (4), enabling the dispensing and control of different types of reagents. The fixture cover plate (9) is connected to the injection pressure plate (11) via an upper spring (53), and the injection pressure plate (26)... 11) Pressed on the top of the sample dispensing control chip stage (4), the bottom of the sample dispensing control chip stage (4) is fitted with a clamp base (1) through a sliding groove, a microfluidic detection chip stage (2) is set between the sample dispensing control chip stage (4) and the clamp base (1), and a microfluidic detection chip (52) is set between the microfluidic detection chip stage (2) and the sample dispensing control chip stage (4); the microfluidic detection chip stage (2) is pressed and sealed with the sample dispensing control chip stage (4) by the lower spring (3) of the clamp base (1); The reagent injection and dispensing control module includes a positive pressure injection device for achieving positive pressure injection and a negative pressure injection device for achieving negative pressure injection. The LCD screen control module includes a circuit board (27), which is connected to the display screen (28).

2. The reagent dispensing control system based on a dual microfluidic chip fixture according to claim 1, characterized in that: The positive pressure injection device includes a plunger pump (12), a flow guide plate (13), a solenoid valve (14), and reagent tubes (15). The solenoid valves (14) are arranged on the flow guide plate (13). The air inlet (16) of the flow guide plate (13) is connected to the plunger pump (12). The air outlet (17) of the flow guide plate (13) is connected to the air inlet pipe (18). The reagent tubes (15) are arranged to pass through the sample outlet pipe (19) and the air inlet pipe (18), respectively. The other end of the sample outlet pipe (19) is connected to the dual microfluidic chip fixture (20). The negative pressure injection device includes a pressure regulating valve (21), a dispensing bottle (22), and a peristaltic pump (23). The sample outlet (25) of the dual microfluidic chip holder (20) is connected to the dispensing bottle (22) through a sample outlet tube. The other end of the dispensing bottle (22) is connected to the pressure regulating valve (21) through a plastic tube. The other side of the pressure regulating valve (21) is connected to the peristaltic pump (23) through a plastic tube.

3. The reagent dispensing control system based on a dual microfluidic chip fixture according to claim 1, characterized in that: The dual microfluidic chip fixture (20) enables the use of two types of microfluidic chips together. The sample dispensing control chip (26) and the microfluidic detection chip (52) are connected in a sealed manner by using the sample dispensing control chip stage (4). The control system can simultaneously perform precise injection control of 6 reagents, and can also push and extract reagents through a positive pressure injection device; The replaceable sample dispensing control chip (26) in the dual microfluidic chip fixture (20) can be selected from different types of chips.

4. The reagent dispensing control system based on a dual microfluidic chip fixture according to claim 2, characterized in that: One end of the air inlet tube (18) and the sample outlet tube (19) connected to the reagent tube (15) is connected to a metal needle (29), and the metal needle (29) of the air inlet tube (18) is located at the rubber part of the reagent tube (15), and the metal needle (29) of the sample outlet tube (19) is located at the bottom of the reagent tube (15).

5. The reagent dispensing control system based on a dual microfluidic chip fixture according to claim 4, characterized in that: The fixture base (1) has a lower deep groove (5) in the middle. The lower deep groove (5) carries the microfluidic detection chip stage (2). The microfluidic detection chip stage (2) is pressed and sealed with the sample dispensing control chip stage (4) by the lower spring (3) of the fixture base (1). The sample dispensing control chip stage (4) is fixed to the lower slide groove (7) on the fixture base (1) by the lower insert plate (6). The sample dispensing control chip stage (4) is fixed to the upper cover plate (9) of the fixture by the upper insert plate (8). The upper cover plate (9) of the fixture has an upper deep groove (10) inside. The sample injection pressure plate (11) is connected by the upper spring (53) and presses the sample injection pressure plate (11) on the top of the sample dispensing control chip stage (4).

6. The reagent dispensing control system based on a dual microfluidic chip fixture according to claim 5, characterized in that: The fixture base (1) has symmetrically machined strip-shaped downward grooves (7) on both sides. The downward grooves (7) are used to fit into the sample dispensing control chip stage (4). A square hole (30) is provided in the lower deep groove (5) of the fixture base (1). The square hole (30) is adapted to the lower boss (31) of the microfluidic detection chip stage (2). The fixture base (1) and the microfluidic detection chip stage (2) are respectively provided with uniformly distributed and corresponding lower spring holes (32). The fixture base (1) and the microfluidic detection chip stage (2) are connected by a lower spring (3). After pressing the microfluidic detection chip stage (2) to compress the lower spring (3), it will move downward. The fixture base (1) is symmetrically machined with a first square groove (33) in the middle of the front and back. The first square groove (33) is used for the sliding limit of the microfluidic detection chip stage (2). The first square groove (33) is located on both sides of the outer wall of the fixture base (1) and a first ear plate (34) is provided. The first ear plate (34) on both sides is machined with a first through hole (35) of different diameter in the middle of the first ear plate (34). The first through hole (35) is used to pass through the first pin (311) and fix the microfluidic detection chip stage (2) to the fixture base (1).

7. The reagent dispensing control system based on a dual microfluidic chip fixture according to claim 6, characterized in that: The microfluidic detection chip stage (2) has a first ear seat (36) on its front and rear sides for cooperating with the first square groove (33). The first ear seat (36) has a first pin hole for passing through the first pin (311). The bottom of the microfluidic detection chip stage (2) has a lower boss (31) in the middle. The bottom of the lower boss (31) has a sample outlet hole (25). The sample outlet hole (25) passes through the lower boss (31) and the microfluidic detection chip stage (2). The sample outlet hole (25) at the bottom of the lower boss (31) is equipped with a first fixing bolt (37). The top of the microfluidic detection chip stage (2) has an opening for a first vacuum suction cup (38). The first vacuum suction cup (38) and the first fixing bolt (37) are connected by a plastic tube.

8. The reagent dispensing control system based on a dual microfluidic chip fixture according to claim 7, characterized in that: The sample dispensing control chip stage (4) has a sample dispensing control chip stage groove (39) in the middle. The sample dispensing control chip stage groove (39) has a hole that passes through the sample dispensing control chip stage (4). A second vacuum suction cup (40) is provided above and below the hole. The second vacuum suction cups (40) are connected to each other by a plastic tube. The sample dispensing control chip stage (4) has an upper insert plate (8) and a lower insert plate (6) symmetrically arranged on the upper and lower sides. The upper insert plate (8) and the lower insert plate (6) are respectively used to be inserted and fixed with the upper cover plate (9) of the fixture and the base plate (1) of the fixture. The sample dispensing control chip stage (4) has a rectangular groove (41) in the center of the front and rear for limiting the movement of the sample dispensing pressure plate (11).

9. The reagent dispensing and control system based on a dual microfluidic chip fixture according to claim 8, characterized in that: The upper cover plate (9) of the fixture is provided with an upper deep groove (10), and square openings (42) are provided on the upper left and right sides of the upper deep groove (10). The square openings (42) are adapted to the upper protrusions (43) on the sample injection pressure plate (11). The upper cover plate (9) of the fixture is symmetrically machined with upper sliding grooves (44) on both sides. The upper sliding grooves (44) are used to fix with the sample injection control chip stage (4). The upper deep groove (10) of the fixture upper cover plate (9) and the upper part of the sample injection pressure plate (11) are also provided with evenly distributed and corresponding upper spring holes (45). The upper cover plate (9) of the fixture and the sample injection pressure plate (11) are connected. The sample pressure plates (11) are connected by an upper spring (53). The upper cover plate (9) of the fixture is symmetrically machined with a second square groove (46) in the middle of the front and back. The second square groove (46) is used for the sliding limit of the sample pressure plate (11). The bottom sides of the front and back second square grooves (46) are provided with second ear plates (47). The middle of the second ear plates (47) on both sides is machined with second through holes (48) of different diameters. The second through holes (48) are used to pass through the second pin (310) and fix the sample pressure plate (11) to the upper cover plate (9) of the fixture. The sample injection plate (11) is provided with second ear seats (49) of the same specifications on the front and rear sides. The second ear seats (49) are symmetrically machined with second pin holes (50) on both sides. The second pin holes (50) are used to pass through the second pin (310). The sample injection plate (11) is provided with upper bosses (43) of the same specifications on the left and right sides. The upper bosses (43) are provided with three evenly distributed sample injection holes (51). The sample injection holes (51) pass through the upper bosses (43) and the sample injection plate (11). A second fixing bolt is installed at the sample injection hole (51) at the top of the upper bosses (43). A third vacuum suction cup is provided at the sample injection hole (51) at the bottom of the sample injection plate (11). The third vacuum suction cup is connected to the second fixing bolt through a plastic tube.

10. The method of using the reagent injection and dispensing control system based on a dual microfluidic chip fixture according to claim 9, characterized in that, Includes the following steps: Step 1: Press down the first ear (36) on both sides of the microfluidic detection chip stage (2) to press down the lower spring (3). After the microfluidic detection chip stage (2) reaches the bottom, insert the two first pins (311) into the first through holes (35) on both sides of the fixture base (1) to fix the microfluidic detection chip stage (2). Then, place the microfluidic detection chip (52) into the chip slot formed by the microfluidic detection chip stage (2) and the fixture base (1). The chip slot plays a limiting role. At this time, the microfluidic detection chip (52) can only move up and down and cannot move around. Step 2: Fix the sample dispensing control chip stage (4) to the sliding groove (7) of the fixture base (1) via the lower insert plate (6). Then, remove the first pins (311) on both sides of the fixture base (1). At this time, the elastic force of the lower spring (3) generates an upward force on the microfluidic detection chip stage (2). Under this force, the microfluidic detection chip stage (2) presses the microfluidic detection chip (52) and connects it to the second vacuum suction cup (40) below the sample dispensing control chip stage (4) via the first vacuum suction cup (38). The microfluidic detection chip stage (2), microfluidic detection chip (52), and sample dispensing control chip stage (4) are sealed together. Then, the sample dispensing control chip (26) is placed in the sample dispensing control chip stage slot (39) according to the flow path, so that the liquid outlet of the sample dispensing control chip (26) is aligned with the through hole of the sample dispensing control chip stage (4). The sample dispensing control chip stage slot (39) plays a limiting role. At this time, the sample dispensing control chip (26) can only move up and down and cannot move around. Step 3: Press the second lugs (49) on both sides of the injection plate (11) upwards. The injection plate (11) will press the upper spring (53) tightly. After the injection plate (11) reaches the top, insert the two second pins (310) into the second through holes (48) of the fixture cover plate (9) respectively to fix the injection plate (11). Then, fix the injection dispensing control chip stage (4) to the upper sliding groove (44) of the fixture cover plate (9) through the upper insert plate (8). Finally, put the fixture cover plate on. When the second pins (310) on both sides of the plate (9) are removed, the spring force of the upper spring (53) exerts a downward force on the injection plate (11). Under this force, the injection plate (11) presses the injection distribution control chip (26) and cooperates with the second vacuum suction cup (40) above the injection distribution control chip stage (4) through the third vacuum suction cup, so that the injection plate (11), the injection distribution control chip (26), and the injection distribution control chip stage (4) are sealed together. Step 4: Turn on the power and press the reagent injection volume and injection sequence on the display screen (28) according to the experimental requirements.

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