Brassica napus a04 chromosome salt-alkali tolerance major gene qtl site, molecular marker and application

Abstract

This invention discloses a major QTL locus on chromosome A04 of Brassica napus (Brassica napus) for salt and alkali tolerance, related molecular markers, and their applications. The QTL locus contributes 4.69% to the salt and alkali tolerance of Brassica napus. The molecular markers were obtained through genome-wide association analysis and include an 11-nucleotide insertion at 8857551 bp, a single-nucleotide mutation at 8857564 bp, a single-nucleotide insertion at 8857584 bp, a single-nucleotide mutation at 8857666 bp, and a three-nucleotide insertion at 8857699 bp on chromosome A04 of the Brassica napus reference genome. This invention can rapidly and accurately detect the haplotype of the major QTL locus for salt and alkali tolerance on chromosome A04 of Brassica napus, which is of great significance for accelerating the molecular breeding process of Brassica napus.

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