一种液质联用同时测定血液样本中29种氨基酸含量的方法
By optimizing chromatographic and mass spectrometric conditions using liquid chromatography-mass spectrometry (LC-MS), the problems of long analysis time and poor stability of amino acids in existing technologies have been solved, enabling accurate quantification of 29 amino acids and their isomers, which is suitable for the precise diagnosis of various diseases.
CN117969708BActive Publication Date: 2026-07-17HUNAN HUAWU XINGCHEN MEDICAL LAB CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- HUNAN HUAWU XINGCHEN MEDICAL LAB CO LTD
- Filing Date
- 2024-01-31
- Publication Date
- 2026-07-17
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Abstract
本发明提供了一种液质联用同时测定血液样本中29种氨基酸含量的方法，包括以下步骤：称取29种氨基酸标准物质分别配制标准曲线，用6‑氨基己酸作内标物质配制内标溶液，取样品，加内标溶液，混匀，加蛋白沉淀剂，再混匀，离心，取上清液氮气吹干，加复溶剂10%乙腈‑10mM甲酸铵溶液，再混匀，离心，取上清液后上机进样分析。该方法可以将多种同分异构体完全分离，比如亮氨酸、异亮氨酸、酪氨酸等异构体，能对各种同分异构体准确定量，更加精准的对各种疾病进行诊断。该方法样品前处理方法简单，分析时间短，节省分析时间，提高分析效率，准确度高、稳定性好、重复性好。
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