A seed treatment suspension concentrate containing metalaxyl-m, ipconazole and fludioxonil

Abstract

The application relates to the technical field of pesticides, and particularly discloses a seed treatment suspending agent containing mefenoxam, ipconazole and fludioxonil, which is used to solve the problems that mefenoxam, ipconazole and fludioxonil are prone to drug resistance when used alone for a long time, the drug amount is increased, the prevention and treatment effect is reduced, the effective period is shortened, a large amount of use causes environmental pollution, and sustainable development is not conducive; the seed treatment suspending agent is prepared by reasonably compounding mefenoxam, ipconazole and fludioxonil with auxiliary materials, has good heat storage stability and low-temperature stability, has a high suspending rate of 99.2%, can obviously improve the drug resistance of seeds and the resistance range of the seeds to pathogenic bacteria after seed coating treatment, has the effect of spectrum sterilization, improves the seed germination rate, obviously increases the prevention and treatment effect, reduces the use amount of single pesticide, and reduces environmental pollution.

Description

Technical Field

[0001] This invention relates to the field of pesticide technology, specifically to a seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil. Background Technology

[0002] Metalaxyl is a broad-spectrum fungicide, belonging to the systemic aniline class of compounds. It has strong bactericidal and fungal growth-inhibiting abilities, and its structural formula is shown below:

[0003]

[0004] The mechanism of action of metalaxyl is mainly to inhibit the biosynthesis of RNA, thereby preventing fungal sporulation and inhibiting mycelial growth, ultimately leading to the death of the pathogen. It has a very good preventive effect on seed-borne and soil-borne diseases caused by lower fungi such as Pythium and Amygdalin.

[0005] Izoxystrobin is a triazole fungicide, an inhibitor of ergosterol biosynthesis, and possesses systemic, protective, and curative effects. Its structural formula is shown below:

[0006]

[0007] The mechanism of action of tebuconazole is to inhibit the synthesis of ergosterol in pathogenic fungi at different sites of action, leading to cell membrane damage and death. It can be used in rice, cereals, ornamental plants and non-crop fields to control many seed diseases, especially effective against rice bakanae disease, leaf spot and rice blast, and can also effectively control stem rot and corn smut in the seedling stage.

[0008] Fludioxonil is a non-systemic broad-spectrum fungicide with both bacteriostatic and bactericidal effects. It exhibits no cross-resistance with existing fungicides and is a highly effective, low-toxicity contact fungicide. Its structural formula is shown below:

[0009]

[0010] The mechanism of action of fludioxonil is mainly through inhibiting the transfer associated with glucose phosphorylation, thereby inhibiting the growth of fungal mycelium and ultimately leading to the death of the pathogen. The fungicidal mechanism against Botrytis cinerea is mainly through dissolving the cell wall of Botrytis cinerea and rapidly destroying the hydrophobic chains on the cell membrane of Botrytis cinerea. It also oxidizes and dissolves proteins, which are the main substances for the life activities of the pathogen, and disrupts the synthesis of proteins and nucleic acids. This interferes with and destroys the biosynthesis and biooxidation processes of Botrytis cinerea. This mechanism of action has uniqueness and a long duration of action, and it is widely used in seed treatment to prevent the damage caused by seed-borne pathogens and soil spread.

[0011] Seeds and seedlings are susceptible to damage and infection from various underground and above-ground pathogens, making them the most vulnerable link in the crop growth process. When seeds and seedlings are infected by pathogens, diseased seedlings and seedlings will appear, reducing the effective number of plants per unit area, resulting in large-scale crop yield reduction and quality decline.

[0012] With advancements in seed treatment technology and increased environmental awareness, the application of agricultural chemicals is increasingly shifting from foliar application to seed treatment. Seed treatment agents containing single active ingredients are frequently used, but their actual effectiveness is poor and their range of action is narrow. Although fungicides such as metalaxyl, cymoxanil, and fludioxonil are highly effective, long-term use as single agents can easily lead to resistance, resulting in increased dosage, reduced efficacy, and shorter duration of action, which is detrimental to sustainable environmental development. Therefore, there is an urgent need for a new type of product to replace seed treatment products with single active ingredients, increasing efficacy, delaying disease progression, and reducing economic losses. Summary of the Invention

[0013] In view of the above situation and to overcome the defects of the prior art, the present invention provides a seed treatment suspension containing metalaxyl, tebuconazole, and fludioxonil: by rationally compounding metalaxyl, tebuconazole, and fludioxonil, the prepared seed treatment suspension has the advantages of all three, with a significant synergistic effect. This solves the problems that long-term use of metalaxyl, tebuconazole, and fludioxonil alone can easily lead to drug resistance, resulting in increased dosage, reduced efficacy, shortened effective period, and environmental pollution caused by large-scale use, which is detrimental to sustainable development.

[0014] To achieve the above objectives, the present invention provides the following technical solution:

[0015] A seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, comprising active ingredients and excipients, wherein the active ingredients comprise 30-60% by mass, and the remainder comprises excipients;

[0016] The active ingredients include metalaxyl, cyproconazole, and fludioxonil;

[0017] The mass ratio of metalaxyl, cyproconazole, and fludioxonil is 20-80:1-30:20-50.

[0018] As a further aspect of the present invention, the mass ratio of metalaxyl, cyprodinil, and fludioxonil is 40-60:10-20:30-40.

[0019] As a further aspect of the present invention, the mass ratio of the metalaxyl, cymoxanil, and fludioxonil is 50:15:35.

[0020] As a further embodiment of the present invention: the excipients include 2-5% sodium dodecylbenzenesulfonate, 2-5% sodium lignosulfonate, 2-5% gelatin, 1.5-2.5% ethylene glycol, 0.3-0.9% zirconium oxide beads, and the balance being deionized water.

[0021] As a further aspect of the present invention: a method for preparing a seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, comprising the following steps:

[0022] S1: Weigh out metalaxyl, cymoxanil and fludioxonil in a mass ratio of 50:15:35 to obtain the active ingredients, and set aside for later use;

[0023] S2: Weigh out 2-5% sodium dodecylbenzenesulfonate, 2-5% sodium lignosulfonate, 2-5% gelatin, 1.5-2.5% ethylene glycol, and 0.3-0.9% zirconium oxide beads according to the mass percentage, then add deionized water to make up to 100% to obtain the auxiliary material, which is ready for use.

[0024] S3: Weigh out 30-60% of the active ingredient by mass percentage, then weigh out the excipients to make up to 100%, and set aside.

[0025] S4: Add metalaxyl, cymoxanil, fludioxonil, sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads and deionized water to a high-speed shear disperser and shear for 5-12 minutes at a shear rate of 5000-10000 r / min to obtain a mixture.

[0026] S5: Add the mixture to a sand mill and grind it until the particle size is 1-5μm to obtain the seed treatment suspension.

[0027] As a further aspect of the present invention: the seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil is used for the treatment of rice, corn, and cotton seeds.

[0028] The beneficial effects of this invention are:

[0029] This invention discloses a seed treatment suspension containing metalaxyl, tebuconazole, and fludioxonil. By rationally combining these three ingredients, the effective components are obtained, resulting in a seed treatment suspension that simultaneously possesses the advantages of all three. It can inhibit fungal sporulation and mycelial growth by suppressing RNA biosynthesis; it can inhibit ergosterol synthesis within pathogenic fungi, leading to cell membrane damage and death; and it can inhibit glucose phosphorylation-related translocation, thereby inhibiting fungal mycelial growth and ultimately causing pathogen death. The combination of these three ingredients has a synergistic effect, significantly improving seed germination rate. It not only reduces the amount of each single active ingredient used, thus reducing environmental pollution and avoiding resistance developed from long-term use alone, but also improves the efficacy against pathogen infection in seeds and extends the duration of its effect. The efficacy is enhanced by the following: the active ingredients are obtained by weighing metalaxyl, cymoxanil, and fludioxonil; excipients are obtained by weighing sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads, and deionized water; the active ingredients and excipients are then mixed evenly and ground to obtain a seed treatment suspension. This seed treatment suspension has good thermal storage stability and low temperature stability, with a suspension rate as high as 99.2%. After treating cotton seeds with the seed treatment suspension and then planting them, the germination rate of the cotton seeds after planting can reach more than 92%, the control effect of Verticillium wilt can reach more than 92% after 90 days, and the control effect of Verticillium wilt can reach more than 85% after 120 days. While reducing the amount of single agent used, it still has a good efficacy duration. These three ingredients cannot be eliminated or replaced. Detailed Implementation

[0030] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0031] Example 1:

[0032] This embodiment describes a method for preparing a seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, comprising the following steps:

[0033] Step 1: Weigh out metalaxyl, cymoxanil and fludioxonil in a mass ratio of 50:15:35 to obtain the active ingredients, and set aside for later use;

[0034] Step 2: Weigh out 2% sodium dodecylbenzenesulfonate, 2% sodium lignosulfonate, 2% gelatin, 1.5% ethylene glycol, and 0.3% zirconium oxide beads according to the mass percentage, then add deionized water to make up to 100% to obtain the auxiliary materials for later use;

[0035] Step 3: Weigh out 30% of the active ingredient according to the mass percentage, then weigh out the excipients to make up to 100%, and set aside;

[0036] Step 4: Add metalaxyl, cymoxanil, fludioxonil, sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads, and deionized water to a high-speed shear disperser and shear for 5 minutes at a shear rate of 5000 r / min to obtain a mixture.

[0037] Step 5: Add the mixture to a sand mill and grind it until the particle size is 1μm to obtain the seed treatment suspension.

[0038] Example 2:

[0039] This embodiment describes a method for preparing a seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, comprising the following steps:

[0040] Step 1: Weigh out metalaxyl, cymoxanil and fludioxonil in a mass ratio of 50:15:35 to obtain the active ingredients, and set aside for later use;

[0041] Step 2: Weigh out 3% sodium dodecylbenzenesulfonate, 3% sodium lignosulfonate, 3% gelatin, 1.8% ethylene glycol, and 0.4% zirconium oxide beads according to the mass percentage, then add deionized water to make up to 100% to obtain the auxiliary materials for later use;

[0042] Step 3: Weigh out 38% of the active ingredient according to the mass percentage, then weigh out the excipients to make up to 100%, and set aside;

[0043] Step 4: Add metalaxyl, cymoxanil, fludioxonil, sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads, and deionized water to a high-speed shear disperser and shear for 6 minutes at a shear rate of 6000 r / min to obtain a mixture.

[0044] Step 5: Add the mixture to a sand mill and grind it until the particle size is 2μm to obtain the seed treatment suspension.

[0045] Example 3:

[0046] This embodiment describes a method for preparing a seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, comprising the following steps:

[0047] Step 1: Weigh out metalaxyl, cymoxanil and fludioxonil in a mass ratio of 50:15:35 to obtain the active ingredients, and set aside for later use;

[0048] Step 2: Weigh out 4% sodium dodecylbenzenesulfonate, 4% sodium lignosulfonate, 4% gelatin, 2% ethylene glycol, and 0.6% zirconium oxide beads according to the mass percentage, then add deionized water to make up to 100% to obtain the auxiliary materials for later use;

[0049] Step 3: Weigh out 48% of the active ingredient according to the mass percentage, then weigh out the excipients to make up to 100%, and set aside;

[0050] Step 4: Add metalaxyl, cymoxanil, fludioxonil, sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads, and deionized water to a high-speed shear disperser and shear for 8 minutes at a shear rate of 8000 r / min to obtain a mixture.

[0051] Step 5: Add the mixture to a sand mill and grind it until the particle size is 3μm to obtain the seed treatment suspension.

[0052] Example 4:

[0053] This embodiment describes a method for preparing a seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, comprising the following steps:

[0054] Step 1: Weigh out metalaxyl, cymoxanil and fludioxonil in a mass ratio of 50:15:35 to obtain the active ingredients, and set aside for later use;

[0055] Step 2: Weigh out 5% sodium dodecylbenzenesulfonate, 5% sodium lignosulfonate, 5% gelatin, 2.3% ethylene glycol, and 0.8% zirconium oxide beads according to the mass percentage, then add deionized water to make up to 100% to obtain the auxiliary materials for later use;

[0056] Step 3: Weigh out 54% of the active ingredient according to the mass percentage, then weigh out the excipients to make up to 100%, and set aside;

[0057] Step 4: Add metalaxyl, cymoxanil, fludioxonil, sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads, and deionized water to a high-speed shear disperser and shear for 10 minutes at a shear rate of 9000 r / min to obtain a mixture.

[0058] Step 5: Add the mixture to a sand mill and grind it until the particle size is 4μm to obtain the seed treatment suspension.

[0059] Example 5:

[0060] This embodiment describes a method for preparing a seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, comprising the following steps:

[0061] Step 1: Weigh out metalaxyl, cymoxanil and fludioxonil in a mass ratio of 50:15:35 to obtain the active ingredients, and set aside for later use;

[0062] Step 2: Weigh out 5% sodium dodecylbenzenesulfonate, 5% sodium lignosulfonate, 5% gelatin, 2.5% ethylene glycol, and 0.9% zirconium oxide beads according to the mass percentage, then add deionized water to make up to 100% to obtain the auxiliary materials for later use;

[0063] Step 3: Weigh out 60% of the active ingredient according to the mass percentage, then weigh out the excipients to make up to 100%, and set aside;

[0064] Step 4: Add metalaxyl, cymoxanil, fludioxonil, sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads, and deionized water to a high-speed shear disperser and shear for 12 minutes at a shear rate of 10,000 r / min to obtain a mixture.

[0065] Step 5: Add the mixture to a sand mill and grind it until the particle size is 5μm to obtain the seed treatment suspension.

[0066] According to the testing standards for seed treatment suspensions, the pH value, suspension rate, viscosity, thermal storage stability, and low-temperature stability of the seed treatment suspensions prepared in Examples 1-5 were tested. The test results are shown in the table below:

[0067] Example 1 6.5 97.8 352 qualified qualified Example 2 6.5 98.2 360 qualified qualified Example 3 6.7 98.4 362 qualified qualified Example 4 6.6 98.8 370 qualified qualified Example 5 6.8 99.2 375 qualified qualified

[0068] Referring to the data in the table above, it can be seen that the seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil in this invention exhibits excellent pH value, suspension rate, viscosity, thermal storage stability, and low-temperature stability.

[0069] Comparative Example 1:

[0070] The difference between Comparative Example 1 and Example 5 is that no nail cream was added.

[0071] Comparative Example 2:

[0072] The difference between Comparative Example 2 and Example 5 is that no tebuconazole was added.

[0073] Comparative Example 3:

[0074] The difference between Comparative Example 3 and Example 5 is that no fludioxonil was added.

[0075] The seed treatment suspensions from Examples 1-5 and Comparative Examples 1-3 were used to coat cotton seeds for field efficacy trials. The experimental fields were sandy loam soil with an organic matter content of 0.85%. Cotton was sown with a row spacing of 80 cm and a plant spacing of 25 cm in each sample plot, with 4 rows planted per plot and 120 seedlings per plot. Germination rate was assessed on the 10th day after sowing, and the control effect on cotton Verticillium wilt was assessed on the 90th and 120th days after sowing. The results are shown in the table below.

[0076] Example 1 92.36 93.45 86.32 Example 2 93.51 95.78 88.56 Example 3 94.28 97.85 89.54 Example 4 96.01 96.26 88.15 Example 5 95.47 98.12 90.45 Comparative Example 1 81.52 84.25 78.29 Comparative Example 2 78.49 80.69 75.03 Comparative Example 3 75.52 76.82 63.74

[0077] Referring to the data in the table above, and comparing Examples 1-5 and Comparative Examples 1-3, it can be seen that the seed treatment composition of metalaxyl, tebuconazole, and fludioxonil can effectively improve the germination rate of cotton seeds and the control efficacy against Verticillium wilt caused by pathogen infection. When one of the three components is missing from the seed treatment composition, the germination rate and the control efficacy against Verticillium wilt caused by pathogen infection are significantly reduced. This indicates that the seed treatment composition of metalaxyl, tebuconazole, and fludioxonil has the best protective effect on seeds, and the effect of using the three components cannot be eliminated or replaced.

[0078] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0079] The above description is merely an example and illustration of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the invention or exceed the scope defined in the claims, all of which should fall within the protection scope of the present invention.

Claims

1. A seed treatment suspension containing metalaxyl, cymoxanil, and fludioxonil, characterized in that: It includes active ingredients and excipients, wherein the active ingredients account for 30-60% by mass percentage, and the remainder is excipients; The active ingredients include metalaxyl, cyproconazole, and fludioxonil; The mass ratio of metalaxyl, cyproconazole, and fludioxonil is 50:15:35; The excipients include 2-5% sodium dodecylbenzenesulfonate, 2-5% sodium lignosulfonate, 2-5% gelatin, 1.5-2.5% ethylene glycol, 0.3-0.9% zirconium oxide beads, and the balance being deionized water; Preparation method of seed treatment suspension Includes the following steps: S1: Weigh out metalaxyl, cymoxanil and fludioxonil in a mass ratio of 50:15:35 to obtain the active ingredients, and set aside for later use; S2: Weigh out 2-5% sodium dodecylbenzenesulfonate, 2-5% sodium lignosulfonate, 2-5% gelatin, 1.5-2.5% ethylene glycol, and 0.3-0.9% zirconium oxide beads according to the mass percentage, then add deionized water to make up to 100% to obtain the auxiliary materials for later use; S3: Weigh out 30-60% of the active ingredient by mass percentage, then weigh out the excipients to make up to 100%, and set aside. S4: Add metalaxyl, cymoxanil, fludioxonil, sodium dodecylbenzenesulfonate, sodium lignosulfonate, gelatin, ethylene glycol, zirconium oxide beads and deionized water to a high-speed shear disperser and shear for 5-12 minutes at a shear rate of 5000-10000 r / min to obtain a mixture. S5: Add the mixture to a sand mill and grind it until the particle size is 1-5μm to obtain the seed treatment suspension.

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