一种高纯度几丁寡糖的制备方法
By acetylation of chitosan oligosaccharides and separation and purification by ion-exchange chromatography, combined with ammonia treatment, the problem of preparing high-purity chitosan oligosaccharides was solved, and the preparation of high-purity chitosan oligosaccharides was realized, which is suitable for bioactivity research and quality control.
CN117986402BActive Publication Date: 2026-07-17INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- INST OF OCEANOLOGY - CHINESE ACAD OF SCI
- Filing Date
- 2024-01-26
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing technologies make it difficult to obtain high-purity chitosan oligosaccharides, leading to inconsistent results in bioactivity studies. Furthermore, impurities such as chitosan oligosaccharides and O-acetylated chitosan oligosaccharides are difficult to remove during the preparation process.
Method used
After acetylation of chitosan oligosaccharides, high-purity chitosan oligosaccharides were prepared by separation and purification at a specific pH value using an ion exchange chromatography column, followed by ammonia treatment to remove impurities.
Benefits of technology
The prepared chitosan oligosaccharide has a purity of over 99.5%, uniform degree of polymerization distribution, low cost, and is suitable for bioactivity screening and quality control.
✦ Generated by Eureka AI based on patent content.
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Figure CN117986402B_ABST
Abstract
本发明公开了一种高纯度几丁寡糖的制备方法，包括如下步骤：将壳寡糖进行乙酰化反应后，得到N‑乙酰化壳寡糖混合物；将所得N‑乙酰化壳寡糖混合物溶解后，用离子交换色谱柱在pH为3‑6下进行分离纯化，收集含有中性寡糖的洗脱液组分；将洗脱液浓缩，冷冻干燥后加入氨水，搅拌反应4‑8h；用离子交换色谱柱在pH为3‑6下进行分离纯化，冷冻干燥，即得高纯度几丁寡糖。本发明所公开的方法可制得高纯度的几丁寡糖，成本低，制备方法操作简便，重复性好，可用于进一步几丁寡糖的生物活性筛选以及单一聚合度几丁寡糖标准品的分离纯化，对于几丁寡糖类生物制品的开发及精准质量控制均具有重要意义。
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