Liquid path system

Abstract

The application discloses a kind of liquid path systems, liquid path system includes independent first reservoir and second reservoir, rotary valve, pump assembly and reaction device. Independent first reservoir and second reservoir;First reservoir can be communicated with rotary valve by first flow path, second reservoir can be communicated with rotary valve by second flow path;Pump assembly is fluidly connected with rotary valve by third flow path;Reaction device is fluidly connected with rotary valve by fourth flow path;Liquid path system is selectively in first state or second state, when liquid path system is in first state, first reservoir is communicated with pump assembly by first flow path, rotary valve and third flow path, and pump assembly is used to drive liquid in first reservoir into third flow path;When liquid path system is in second state, second reservoir is communicated with reaction device by second flow path, rotary valve and fourth flow path, and pump assembly is used to drive liquid in second reservoir into reaction device.

Description

Technical Field

[0001] The embodiments of the present invention relate to the technical field of gene sequencing, and more particularly to a liquid circuit system. Background Technology

[0002] The liquid chromatography system of biochemical analyzers (such as gene sequencers) is used to sequence different reagents. After sequencing, reagent residue often remains in the reagent needles used to extract reagents or in the tubing supplying reagent flow. If this residue is not cleaned promptly, it can affect the instrument's performance if the instrument is left idle for a period of time. Summary of the Invention

[0003] This invention provides a liquid circuit system.

[0004] The fluid circuit system of this invention includes:

[0005] Independent first and second memory;

[0006] A rotary valve, wherein the first memory is connected to the rotary valve via a first flow path, and the second memory is connected to the rotary valve via a second flow path;

[0007] A pump assembly, which is fluidly connected to the rotary valve via a third flow path;

[0008] A reaction apparatus, wherein the reaction apparatus is fluidly connected to the rotary valve via a fourth flow path;

[0009] The liquid circuit system is selectively in a first state or a second state. When the liquid circuit system is in the first state, the first memory is connected to the pump assembly through the first flow path, the rotary valve and the third flow path. The pump assembly is used to drive the liquid in the first memory into the third flow path.

[0010] When the liquid circuit system is in the second state, the second storage device is connected to the reaction device through the second flow path, the rotary valve and the fourth flow path, and the pump assembly is used to drive the liquid in the second storage device into the reaction device.

[0011] In the liquid path system of this invention, the system is configured to switch between a first state and a second state, with the liquid entering the third flow path or reaction device in different states. The system can perform different functions when different liquids are stored in the first and second memories. Furthermore, when the first and second memories store sequencing reagents and cleaning solution respectively, the system can simultaneously perform sequencing and cleaning functions. After sequencing, the system can be cleaned promptly, ensuring no sequencing reagent residue remains and guaranteeing instrument performance.

[0012] In some embodiments, the fluid system includes a multi-way valve disposed between the rotary valve and the reaction device, the multi-way valve changing its own passage to allow the fluid system to be in either the first state or the second state.

[0013] In some embodiments, the multi-port valve includes a first port, a second port, and a third port, wherein the first port is selectively connected to either the second port or the third port, the first port is connected to the rotary valve, the second port is connected to one end of the third flow path, the other end of the third flow path is connected to the pump assembly, the third port is connected to one end of the fourth flow path, and the other end of the fourth flow path is connected to the reaction device;

[0014] When the first port is connected to the second port, the liquid circuit system is in the first state; when the first port is connected to the third port, the liquid circuit system is in the second state.

[0015] In some embodiments, the rotary valve includes a common port and a plurality of connection ports, the common port being selectively connected to at least one of the connection ports, the common port being connected to the first port, and the first flow path and the second flow path being respectively connected to the corresponding connection ports;

[0016] When the rotary valve is in the first valve position, the common port is connected to the first flow path through at least one of the connection ports;

[0017] When the rotary valve is in the second valve position, the common port is connected to the second flow path through the remaining connection ports.

[0018] In some embodiments, the rotary valve has a first common port, a second common port, a plurality of connection ports, a first connecting groove and a second connecting groove, the first common port being connected to one end of the third flow path, the other end of the third flow path being connected to the pump assembly, the second common port being connected to one end of the fourth flow path, and the other end of the fourth flow path being connected to the reaction device;

[0019] When the rotary valve is in the first valve position, the first common port is connected to the first flow path through at least one of the connection ports and the first communication slot;

[0020] When the rotary valve is in the second valve position, the second common port is connected to the second flow path through the remaining connection ports and the second communication slot.

[0021] In some embodiments, the rotary valve includes a stator and a rotor disposed opposite to the stator, the stator having a first common port, a second common port, the plurality of connection ports and at least a portion of the first communicating groove, and the rotor having at least a portion of the second communicating groove.

[0022] In some embodiments, the first connecting slot includes a first slot and a second slot that are interconnected, the first slot and the second slot being respectively disposed on the stator and the rotor.

[0023] In some embodiments, the first slot has two ends, one end of which is connected to the first common port and the other end of which is connected to the second slot. The second slot has two ends, one end of which is connected to the first slot and the other end of which may be connected to one of the connection ports.

[0024] In some embodiments, the stator includes a first end face and a second end face facing away from each other, and the rotor includes a third end face and a fourth end face facing away from each other, the second end face and the third end face being in contact, the first groove being formed on the first end face, and the second groove being formed on the third end face.

[0025] In some embodiments, the second communication slot has two ends, one end of which is connected to the second common port, and the other end is optionally connected to one of the connection ports.

[0026] In some embodiments, the stator includes a first end face and a second end face facing away from each other, and the rotor includes a third end face and a fourth end face facing away from each other, the second end face and the third end face being in contact, and the second connecting groove being formed on the third end face.

[0027] In some embodiments, when the fluid system is in the first state, the pump assembly further controls the liquid in the third flow path to flow back to the second flow path to clean the second flow path.

[0028] In some embodiments, the liquid circuit system includes a first reagent needle and a first driving mechanism, the first reagent needle being connected to the first flow path and / or the second flow path, and the first driving mechanism being used to drive the first reagent needle to move along the length direction of the first reagent needle so that the first reagent needle extends into the first memory and / or the second memory.

[0029] In some embodiments, the first drive mechanism includes:

[0030] Fixture;

[0031] A movable component is movably disposed on the fixed frame, and the first reagent needle is mounted on the movable component;

[0032] A first drive assembly is disposed on the fixed frame, the first drive assembly being used to drive the movable part to move relative to the fixed frame.

[0033] In some embodiments, the fixing frame includes a first plate, a second plate, and a connecting column, the first plate and the second plate being spaced apart, the connecting column connecting the first plate and the second plate, the movable member being movably sleeved on the connecting column, and the first drive assembly being mounted on the first plate and the second plate.

[0034] In some embodiments, the first drive assembly includes a first motor and a first lead screw connected to the first motor, the movable part is sleeved on the first lead screw, and the first motor drives the movable part to move through the first lead screw.

[0035] In some embodiments, the fluid circuit system includes a second reagent needle and a mounting base, the mounting base being mounted on the fixture, the second reagent needle being connected to the first flow path and / or the second flow path, the second reagent needle being mounted on the mounting base, and the second reagent needle being inserted into the first memory and / or the second memory when the movable element moves toward the first memory and / or the second memory.

[0036] In some embodiments, the mounting base includes a guide and a slider. The guide is mounted on the fixed frame, and the slider is movably disposed on the guide. The second reagent needle is mounted on the slider, and the slider moves when the movable member moves toward the first memory and / or the second memory.

[0037] In some embodiments, a first elastic element is provided between the slider and the fixed frame. The first elastic element compresses and stores force when the movable element moves toward the first memory and / or the second memory, and releases the stored force after the force between the movable element and the slider is eliminated, so that the second reagent needle moves toward the outside of the first memory and / or the second memory.

[0038] In some embodiments, a second elastic member is connected between the slider and the second reagent needle. The second elastic member applies a force in a first direction to the second reagent needle to keep the second reagent needle on the slider. When the second reagent needle is subjected to a force in a second direction, the second elastic member is compressed under the action of the second reagent needle. The first direction is the direction in which the second reagent needle is directed toward the first memory and / or the second memory, and the second direction is opposite to the first direction.

[0039] In some embodiments, the slider is provided with a mounting hole, the second reagent needle includes a needle body and a flange disposed on the needle body, the needle body passes through the slider, the flange is disposed in the mounting hole, and the second elastic member abuts against the flange.

[0040] In some embodiments, the fluid system includes a first detection component disposed on the fixture, the first detection component being used to detect the position of the moving part.

[0041] In some embodiments, the first detection component includes a first photoelectric switch and a second photoelectric switch arranged at intervals along the movement direction of the movable member, wherein the first photoelectric switch and the second photoelectric switch are used to detect the upper limit position and the lower limit position of the movable member, respectively.

[0042] In some embodiments, the fluid system includes a liquid collector for collecting liquids generated by the fluid system, the liquid collector having an inlet, and the fluid system also includes a cover assembly.

[0043] During the process of driving the first reagent needle to extend into the first memory and / or the second memory, the first driving mechanism drives the cover assembly to close and press against the inlet; or,

[0044] During the process of driving the first reagent needle away from the first memory and / or the second memory, the first driving mechanism drives the cover assembly to open the liquid inlet.

[0045] In some embodiments, the first drive mechanism includes:

[0046] Fixture;

[0047] A movable component is movably disposed on the fixed frame, and the first reagent needle is mounted on the movable component;

[0048] A first drive assembly is disposed on the fixed frame. The first drive assembly is used to drive the movable part to move relative to the fixed frame. During the movement of the movable part, the movable part can drive the cover assembly to close and press the liquid inlet or open the liquid inlet.

[0049] In some embodiments, the cover assembly includes a guide rail, a connecting frame, and a pressure cap. The guide rail is mounted on the fixed frame, the connecting frame is movably disposed on the guide rail, and the pressure cap is disposed on the connecting frame. During the movement of the movable member, the movable member can drive the connecting frame to cause the pressure cap to close and press against the liquid inlet or open the liquid inlet.

[0050] In some embodiments, the connecting frame is provided with a first limiting position and a second limiting position, which are spaced apart along the movement direction of the connecting frame. When the movable member moves in a first direction, the movable member can press against the first limiting position to close and press the pressure cap against the liquid inlet. When the movable member moves in a second direction, the movable member can abut against the second limiting position to open the liquid inlet. The first direction is the direction in which the first reagent needle faces the first memory and / or the second memory, and the second direction is opposite to the first direction.

[0051] In some embodiments, the connecting frame is provided with a groove that extends along the movement direction of the connecting frame, and the two groove walls along the movement direction of the connecting frame respectively form the first limiting position and the second limiting position.

[0052] In some embodiments, an elastic element is provided between the connecting frame and the pressure cap, with both ends of the elastic element connected to the connecting frame and the pressure cap, respectively.

[0053] In some embodiments, the first memory and the second memory are both located in the same kit, and the liquid collector and the kit are both disposed on the same pull-out device, which is used to be disposed within the housing of the liquid circuit system by a pull-out mechanism.

[0054] In some embodiments, the reagent kit is kept at room temperature.

[0055] In some embodiments, the fluid circuit system further includes a bracket and a second drive mechanism connected to the bracket, wherein the first memory and the second memory are both mounted on the bracket, and the second drive mechanism is used to drive the bracket to move so as to move the first memory and the second memory.

[0056] In some embodiments, the second drive mechanism includes a second motor and a second lead screw connected to the second motor, the second lead screw being connected to the bracket, and the second motor driving the bracket to move via the second lead screw.

[0057] In some embodiments, the fluid system includes a second detection component for detecting the position of the bracket to determine the positions of the first memory and the second memory.

[0058] In some embodiments, the first memory and the second memory are both located in the same reagent kit. The second detection component includes a third photoelectric switch, a fourth photoelectric switch, a first light-blocking element, and a second light-blocking element. The third photoelectric switch and the fourth photoelectric switch are spaced apart above the tray along the movement direction of the tray. The first light-blocking element and the second light-blocking element can both abut against the reagent kit. The first light-blocking element and the second light-blocking element can both move in a movement direction perpendicular to the movement of the reagent kit when the reagent kit moves. When the reagent kit is in a first position, the first light-blocking element blocks the light path of the third photoelectric switch, and the second light-blocking element blocks the light path of the fourth photoelectric switch when the reagent kit is in a second position.

[0059] In some embodiments, the first memory and the second memory are both located in the same kit, and the second detection component includes a fifth photoelectric switch and a third light-blocking element. The third light-blocking element is disposed on the bracket, and when the kit is in the initial position, the third light-blocking element blocks the light path of the fifth photoelectric switch.

[0060] In some embodiments, the first memory and the second memory are both located in the same kit. The second detection component includes a third photoelectric switch, a fourth photoelectric switch, and a first light-blocking element. The third photoelectric switch and the fourth photoelectric switch are spaced apart on the side of the tray along the movement direction of the tray. The first light-blocking element is disposed on the tray. When the kit is in a first position, the first light-blocking element blocks the light path of the third photoelectric switch. When the kit is in a second position, the first light-blocking element blocks the light path of the fourth photoelectric switch.

[0061] In some embodiments, the first memory and the second memory are both located in the same kit, and the second detection component further includes a fifth photoelectric switch and a third light-blocking element. The third light-blocking element is capable of moving in a direction perpendicular to the movement of the kit during the process of placing the kit into the tray, so as to block the light path of the fifth photoelectric switch.

[0062] In some embodiments, the bracket is provided with a limiting structure that restricts the movement of the first memory and the second memory relative to the bracket.

[0063] In some embodiments, the fluid system includes a cooling device, and the bracket houses the first memory and the second memory within the cooling device.

[0064] In some embodiments, the bottom of the bracket has a through hole extending through the bracket.

[0065] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0066] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0067] Figure 1 This is a schematic diagram of the structure of the liquid circuit system according to an embodiment of the present invention;

[0068] Figure 2 This is a partial structural schematic diagram of the rotary valve according to an embodiment of the present invention;

[0069] Figure 3 This is a schematic diagram of the rotor structure according to an embodiment of the present invention;

[0070] Figure 4 This is a schematic diagram of the stator structure according to an embodiment of the present invention;

[0071] Figure 5 This is a schematic diagram of the stator structure according to an embodiment of the present invention;

[0072] Figure 6 This is a schematic diagram of the rotor structure according to an embodiment of the present invention;

[0073] Figure 7 This is a schematic diagram of the structure of the liquid circuit system according to an embodiment of the present invention;

[0074] Figure 8 This is a schematic diagram of the assembly structure of the fixture, reagent kit, and cooling device according to an embodiment of the present invention;

[0075] Figure 9 yes Figure 8 A disassembly diagram of the assembly structure;

[0076] Figure 10 This is a schematic diagram of the assembly structure of the fixing frame and reagent needle according to an embodiment of the present invention;

[0077] Figure 11 This is a schematic diagram of the assembly structure of the second reagent needle and the mounting base according to an embodiment of the present invention;

[0078] Figure 12 yes Figure 11 A sectional view of the assembly structure along direction a;

[0079] Figure 13 This is a partial structural schematic diagram of the liquid circuit system according to an embodiment of the present invention;

[0080] Figure 14 This is a partial structural schematic diagram of the liquid circuit system according to an embodiment of the present invention;

[0081] Figure 15 This is a schematic diagram of the structure of the cover assembly according to an embodiment of the present invention;

[0082] Figure 16 This is a partial structural schematic diagram of the liquid circuit system according to an embodiment of the present invention;

[0083] Figure 17 yes Figure 8 A partial structural diagram of the assembly structure;

[0084] Figure 18 This is a schematic diagram of the structure of the second detection component according to an embodiment of the present invention;

[0085] Figure 19 yes Figure 8 A partial structural diagram of the assembly structure;

[0086] Figure 20 This is a schematic diagram of the bracket according to an embodiment of the present invention;

[0087] Figure 21 This is a schematic diagram of the lower plate according to an embodiment of the present invention.

[0088] Key component symbols: Liquid circuit system 1000; First memory 100; Second memory 200; Rotary valve 300; Pump assembly 400; Reaction device 500; First unit 501; First unit 502; First flow path 10; Second flow path 11; Third flow path 12; Fourth flow path 13; First liquid circuit unit 600; Second liquid circuit unit 700; First manifold 800; First inlet 810; Second inlet 820; First outlet 830; Second outlet 840; Third outlet 850; Fourth outlet 860; Pressure sensor 900; Second manifold 14; First common port 310; Second common port 320; Connection port 330; Common port 301; A connecting channel 340; a second connecting channel 350; a stator 360; a rotor 370; a first channel 341; a second channel 342; a first end 3410 of the first channel; a second end 3411 of the first channel; a first end 3420 of the second channel; a second end 3421 of the second channel; a first end face 361; a second end face 362; a third end face 371; a fourth end face 372; a first end 351 of the second connecting channel; a second end 352 of the second connecting channel; a multi-port valve 15; a first port 150; a second port 151; a third port 152; a first reagent needle 16; a first drive mechanism 17; a first reagent needle 160; a second reagent needle 161; a third reagent needle 162; a third manifold 18; and a liquid extraction port 19. Fixed frame 170; movable part 171; first drive assembly 172; first plate 173; second plate 174; connecting column 175; first motor 1720; first lead screw 1721; second reagent needle 20; mounting base 21; guide 210; sliding part 211; first elastic part 22; paddle 1710; second elastic part 23; mounting hole 2110; needle body 201; flange 202; first detection assembly 24; first photoelectric switch 240; second photoelectric switch 241; support plate 176; light shield 1711; liquid collector 25; liquid inlet 250; cover assembly 26; guide rail 260; connecting frame 261; pressure cap 262; first limiting position 2610; second limiting position 2611; recess 2613; elastic element 27; pull-out device 28; drawer box 280; slide rail 281; partition 2801; reagent kit anti-mistake block 2802; liquid collector anti-mistake block 2803; bracket 29; second drive mechanism 30; second motor 31; second lead screw 32; second detection component 40; third photoelectric switch 41; fourth photoelectric switch 42; first light blocking element 43; second light blocking element 44; first limit block 290; second limit block 291; cooling device 50; box body 51; chamber 510; through hole 292; lower plate 52; receiving groove 520; water outlet 521; strip groove 523; reagent kit 60; liquid manifold 70; drain pipe 71; fifth photoelectric switch 45; third light blocking element 46. Detailed Implementation

[0089] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0090] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more of the stated features. In the description of this invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0091] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, an electrical connection, or a connection that allows for communication; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0092] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0093] The following disclosure provides many different embodiments or examples for implementing various structures of the invention. To simplify the disclosure, specific examples of components and arrangements are described below. These are merely examples and are not intended to limit the invention. Furthermore, reference numerals and / or letters may be repeated in different examples; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various embodiments and / or arrangements discussed. In addition, examples of various specific processes and materials are provided in this invention, but those skilled in the art will recognize the application of other processes and / or the use of other materials.

[0094] Please see Figure 1 The liquid circuit system 1000 of the present invention includes an independent first memory 100 and second memory 200, a rotary valve 300, a pump assembly 400 and a reaction device 500. The first storage device 100 is connected to the rotary valve 300 via the first flow path 10, and the second storage device 200 is connected to the rotary valve 300 via the second flow path 11. The pump assembly 400 is fluidly connected to the rotary valve 300 via the third flow path 12. The reaction device 500 is fluidly connected to the rotary valve 300 via the fourth flow path 13. The liquid system 1000 is selectively in either the first or second state. When the liquid system 1000 is in the first state, the first storage device 100 is connected to the pump assembly 400 via the first flow path 10, the rotary valve 300, and the third flow path 12, and the pump assembly 400 is used to drive the liquid in the first storage device 100 into the third flow path 12. When the liquid system 1000 is in the second state, the second storage device 200 is connected to the reaction device 500 via the second flow path 11, the rotary valve 300, and the fourth flow path 13, and the pump assembly 400 is used to drive the liquid in the second storage device 200 into the reaction device 500.

[0095] In the liquid path system 1000 of this embodiment, the liquid path system 1000 is configured to switch between a first state and a second state. The liquid in the liquid path system 1000 enters the third flow path 12 or the reaction device 500 in different states. When the first memory 100 and the second memory 200 store different liquids, the liquid path system 1000 can have different functions. Furthermore, when the first memory 100 and the second memory 200 respectively store cleaning solution and sequencing reagents, the liquid path system 1000 can simultaneously perform cleaning and sequencing functions. The liquid path system 1000 can be cleaned promptly after sequencing is completed, ensuring no sequencing reagent residue remains in the liquid path system 1000, thus guaranteeing the performance of the instrument.

[0096] Specifically, the first memory 100 and the second memory 200 can be containers for containing liquids. Optionally, the liquid contained in the first memory 100 may include a washing solution. The liquid contained in the second memory 200 may include sequencing reagents. "Sequencing reagents" refers to reagents needed for the detection and analysis of nucleic acids to be tested, such as during sequencing reactions. Sequencing reagents include imaging reagents, excision reagents, etc. "Washing solution" refers to another liquid or substance used to remove or replace it in the previous reaction system.

[0097] Both the first memory 100 and the second memory 200 may include multiple reagent tubes, which are used to hold the same or different liquids. The liquid system 1000 of this embodiment can transport liquids, which can be various types, including reaction solutions, biological sample liquids, buffer solutions, washing solutions, etc., and include reagents for different reactions or different steps of the same reaction. The liquid system 1000 can transport biological sample liquids to the reaction device 500 for corresponding reactions, such as hybridization reactions and sequencing reactions.

[0098] The biological sample liquid is a liquid containing the nucleic acid to be tested. The liquid system 1000 delivers the biological sample liquid to the fluid channel of the reaction device 500, so that the nucleic acid to be tested and at least a part of the probe can be complementaryly paired, that is, a hybridization reaction is carried out, so as to fix or attach the nucleic acid to be tested to the surface of the fluid channel for subsequent detection and analysis of the nucleic acid to be tested, such as sequencing reaction.

[0099] The reaction apparatus 500 has a sandwich-like structure with three layers (top, middle, and bottom), or a two-layer structure. The top layer is a transparent glass layer, and the middle or bottom layer is a transparent or opaque substrate layer. The middle or bottom layer has an array of fluid channels that can contain liquid and provide physical space for the reaction. Probes (oligonucleotides) are placed on the upper surface (lower surface of the upper glass layer) or the lower surface (upper surface of the middle or bottom layer) of the fluid channels.

[0100] Multiple rotary valves 300 can be used, and these multiple rotary valves 300 can integrate the functions of multiple three-way valves. This allows for the diversion of liquid in the liquid circuit system 1000 with a significantly reduced number of valves, thereby reducing the cost, size, and reagent consumption of the liquid circuit system 1000, improving reliability, and facilitating maintenance and operation. The rotary valve 300 can be connected to the first storage device 100 via a first flow path 10, allowing liquid in the first storage device 100 to flow into the rotary valve 300. The rotary valve 300 can also be connected to the second storage device 200 via a second flow path 11, allowing liquid in the second storage device 200 to flow into the rotary valve 300.

[0101] Please see Figure 1In some embodiments, the reaction apparatus 500 may include a first unit 501 and a second unit 502. The number of first units 501 and second units 502 is not limited in this embodiment. Each unit in the first unit 501 and the second unit 502 includes at least one fluid channel. The number of fluid channels in the two units may be the same or different. In this embodiment, both the first unit 501 and the second unit 502 include two fluid channels.

[0102] The liquid path system 1000 can deliver the same or different biological sample liquids to the fluid channels of the first unit 501 and the second unit 502 of the reaction device 500, respectively, to achieve the detection and analysis of the same or different nucleic acids to be tested. Therefore, the liquid path system 1000, including the reaction device 500, is beneficial for improving sequencing throughput and / or enabling the simultaneous detection of multiple samples. To avoid crosstalk when the liquid path system 1000 supplies liquid to the first unit 501 and the second unit 502...

[0103] Please see Figure 1 The liquid circuit system 1000 of the present invention is designed to include a first liquid circuit unit 600 and a second liquid circuit unit 700. The first liquid circuit unit 600 and the second liquid circuit unit 700 are independent and parallel to each other, so as to supply liquid to the first unit 501 and the second unit 502 respectively. This is beneficial for the first unit 501 and the second unit 502 to perform different reactions or different steps of the same reaction, so as to realize the detection and analysis of the same or different nucleic acids to be tested.

[0104] Specifically, both the first liquid circuit unit 600 and the second liquid circuit unit 700 include a reagent switching system and a power system.

[0105] The reagent switching system is located upstream of the reaction apparatus 500. Specifically, the reagent switching system includes a rotary valve 300 and a first manifold 800. The rotary valve 300 controls different liquids to sequentially pass through the first manifold 800 into the fluid channel of the reaction apparatus 500. The first manifold 800 includes a first inlet 810, a second inlet 820, a first outlet 830, a second outlet 840, a third outlet 850, and a fourth outlet 860.

[0106] The first liquid inlet 810 is connected to the first liquid outlet 830 and the second liquid outlet 840, respectively; the second liquid inlet 820 is connected to the third liquid outlet 850 and the fourth liquid outlet 860, respectively. The liquid transported by the first liquid circuit unit 600 flows into the first manifold 800 through the rotary valve 300 of the first liquid circuit unit 600, and is divided into two parts, flowing out from the first liquid outlet 830 and the second liquid outlet 840.

[0107] The first liquid outlet 830 and the second liquid outlet 840 are respectively connected to and correspond one-to-one with the two fluid channels of the first unit 501 on the reaction device 500. The liquid transported by the first liquid circuit unit 600 flows out from the first liquid outlet 830 and the second liquid outlet 840, and then flows into the two fluid channels of the first unit 501, thereby realizing that the first liquid circuit unit 600 supplies liquid to the first unit 501 on the reaction device 500 independently. The liquid transported by the second liquid circuit unit 700 flows into the first manifold 800 through the rotary valve 300 of the second liquid circuit unit 700 and the second liquid inlet 820, where it is divided into two and flows out from the third liquid outlet 850 and the fourth liquid outlet 860.

[0108] The third outlet 850 and the fourth outlet 860 are respectively connected to and correspond one-to-one with the two fluid channels of the second unit 502 on the reaction device 500. After the liquid transported by the second liquid circuit unit 700 flows out from the third outlet 850 and the fourth outlet 860, it flows into the two fluid channels of the second unit 502 respectively, thereby realizing that the second liquid circuit unit 700 supplies liquid to the second unit 502 on the reaction device 500 independently.

[0109] The first liquid path unit 600 and the second liquid path unit 700 independently and in parallel supply liquid to the first unit 501 and the second unit 502 of the reaction device 500. This facilitates different reactions or different steps of the same reaction in the first unit 501 and the second unit 502, enabling the detection and analysis of the same or different nucleic acids to be tested. This improves sequencing throughput and / or allows for the simultaneous detection of multiple samples. It should be noted that there can be multiple rotary valves 300 and first manifolds 800. The illustration is only for illustrative purposes and should not be construed as limiting the number of rotary valves 300 and first manifolds 800 in this embodiment of the invention.

[0110] In some embodiments, the first busbar 800 may be designed separately from the reaction device 500.

[0111] In some embodiments, the first busbar 800 may be integrated with the reaction device 500 on the reaction device 500.

[0112] Please see Figure 1 In some embodiments, the power system provides power to the liquid system 1000, enabling the liquid in the liquid system 1000 to flow.

[0113] Specifically, the power system may include a pump assembly 400, a pressure sensor 900, and a second manifold 14. The pump assembly 400 is located downstream of the reaction device 500 and provides negative pressure for the liquid to pass through the rotary valve 300 and the inflow fluid passage.

[0114] Optionally, the number of pump assemblies 400 is the same as the number of fluid channels in the reaction apparatus 500, thereby enabling the pump assemblies 400 to independently control the liquid in each fluid channel in the reaction apparatus 500, which facilitates precise control of the flow rate and / or velocity of the fluid in each fluid channel.

[0115] Additionally, the pump assembly 400 is located downstream of the reaction apparatus 500 and provides negative pressure to prevent cross-contamination or leakage of liquids in the fluid channels within the reaction apparatus 500. A pressure sensor 900 is installed on the pipeline connecting the reaction apparatus 500 and the pump assembly 400. The pressure sensor 900 monitors the pressure of the liquid system 1000 and issues an alarm when the pressure is abnormal. The second manifold 14 can be used to merge the liquid flowing out of the reaction apparatus 500, or in other words, to merge the liquid generated during sequencing. The liquid generated during sequencing can be the liquid generated after the sequencing reagents react, or the used sequencing reagents, or other liquids used to maintain the reliability of the sequencing process, such as condensate used to cool the sequencing environment.

[0116] The number of pump assembly 400, pressure sensor 900 and second manifold 14 can be multiple. The illustration is only for illustrative purposes and should not be taken as a limitation on the pump assembly 400, pressure sensor 900 and second manifold 14 in the embodiments of the present invention.

[0117] The switching between the first and second states of the liquid system 1000 can be achieved via the pump assembly 400 and / or the rotary valve 300. For example, when the liquid system 1000 is in the first state, the pump assembly 400 can drive the cleaning fluid in the first storage 100 into the first flow path 10, the rotary valve 300, and the third flow path 12. In this document, "cleaning" refers to the introduction of a liquid to remove or replace another liquid or substance in the previous reaction system, and generally does not involve substantial treatment and / or biochemical reactions.

[0118] When the liquid system 1000 is in the second state, the pump assembly 400 can drive the sequencing reagents in the second memory 200 to enter the reaction device 500 through the second flow path 11, the rotary valve 300 and the fourth flow path 13 for sequencing reaction.

[0119] Please see Figure 1 and Figure 2In some embodiments, the rotary valve 300 is provided with a first common port 310, a second common port 320, a plurality of connection ports 330, a first connecting groove 340 and a second connecting groove 350. The first common port 310 is connected to one end of the third flow path 12, and the other end of the third flow path 12 is connected to the pump assembly 400. The second common port 320 is connected to one end of the fourth flow path 13, and the other end of the fourth flow path 13 is connected to the reaction device 500. When the rotary valve 300 is in the first valve position, the first common port 310 is connected to the first flow path 10 through at least one of the connection ports 330 and the first connecting groove 340. When the rotary valve 300 is in the second valve position, the second common port 320 is connected to the second flow path 11 through the remaining connection ports 330 and the second connecting groove 350.

[0120] Thus, by configuring the rotary valve 300 to switch between a first valve position and a second valve position, in different positions, the first common port 310 and at least one of the connection ports 330 can be connected to the first flow path 10 through the first connecting groove 340, thereby enabling the first storage container 100 to supply liquid to the third flow path 12. The second common port 320 and the remaining connection ports 330 are connected to the second flow path 11 through the second connecting groove 350, thereby enabling the second storage container 200 to supply liquid to the fourth flow path 13. This configuration integrates the function of a three-way valve into the rotary valve 300 and provides two common ports, simplifying the liquid circuit system 1000 and reducing reagent consumption.

[0121] The first common port 310 can be connected to the connection port 330 through the first connecting groove 340. The figure of the embodiment of the present invention shows one first common port 310. The first common port 310 can serve as the liquid inlet or outlet of the rotary valve 300.

[0122] Alternatively, liquid can enter or exit the rotary valve 300 through the first common port 310, thereby achieving flow splitting or parallel flow. Here, the first common port 310 serves as the liquid inlet of the rotary valve 300, allowing liquid to enter the rotary valve 300 from the first common port 310. The shape of the first common port 310 can be a regular shape such as a circle or a polygon, or it can be an irregular shape.

[0123] Similarly, the second common port 320 can be connected to the connection port 330 via the second connecting groove 350. The illustration in this embodiment shows one second common port 320. The second common port 320 can serve as a liquid inlet or outlet of the rotary valve 300. In other words, liquid can enter or exit the rotary valve 300 through the second common port 320, thereby achieving flow splitting or parallel flow. Here, the second common port 320 serves as the liquid inlet of the rotary valve 300, allowing liquid to enter the rotary valve 300. The shape of the second common port 320 can be a regular shape such as a circle or polygon, or it can be an irregular shape. In this embodiment, to facilitate the formation, manufacturing, and / or connection with common pipes, the second common port 320 is circular.

[0124] To facilitate the arrangement of the connection port 330, the included angle between the first connecting groove 340 and the second connecting groove 350 can be set to 173.5°, that is, the angle between the extension segment of the first connecting groove 340 through the axis of the rotary valve 300 and the extension segment of the second connecting groove 350 through the axis of the rotary valve 300. This embodiment of the invention does not limit the size of the included angle between the first connecting groove 340 and the second connecting groove 350. Furthermore, by adjusting the angle and shape between the first connecting groove 340 and the second connecting groove 350, the positions of the first common port 310 and the second common port 320 on the rotary valve 300 can be adjusted.

[0125] Only one of the first common port 310 and the second common port 320 can be connected to the connection port 330, while the other port is blocked. Alternatively, when the first common port 310 and the connection port 330 are connected through the first connecting groove 340, the second common port 320 is blocked (i.e., liquid cannot flow into the second common port 320); when the second common port 320 and the connection port 330 are connected through the second connecting groove 350, the first common port 310 is blocked (i.e., liquid cannot flow into the first common port 310).

[0126] When the rotary valve 300 is rotated to the first valve position, the first common port 310 is connected to the multiple connection ports 330 through the first connecting groove 340. At this time, liquid enters the rotary valve 300 from the first common port 310 and flows out through the multiple connection ports 330. When the rotary valve 300 is rotated to the second valve position, the second common port 320 is connected to the multiple connection ports 330 through the second connecting groove 350. At this time, liquid enters the rotary valve 300 from the second common port 320 and flows out through the multiple connection ports 330.

[0127] Please see Figure 1 and Figure 2In some embodiments, the rotary valve 300 includes a stator 360 and a rotor 370 disposed opposite to the stator 360. The stator 360 is provided with a first common port 310, a second common port 320, a plurality of connection ports 330 and at least a portion of a first connecting groove 340, and the rotor 370 is provided with at least a portion of a second connecting groove 350.

[0128] Thus, during the rotation of the rotor 370, the first connecting groove 340 and the second connecting groove can connect the first common port 310 and the second common port 320, so that liquid can enter the rotary valve 300 from the stator 360 and flow out of the rotary valve 300 from the stator 360.

[0129] The rotor 370 and stator 360 are coaxially arranged, or in other words, the central axis of the rotor 370 coincides with the central axis of the stator 360, and the second common port 320 can be located on the central axis. It can be understood that the rotor 370 can rotate relative to the stator 360. Furthermore, the rotor 370 can rotate relative to the stator 360 between a first valve position and a second valve position.

[0130] It is worth noting that the rotary valve 300 rotates between the first valve position and the second valve position, that is, the rotor 370 of the rotary valve 300 rotates itself, rather than the rotary valve 300 as a whole rotating.

[0131] The fact that the stator 360 is provided with at least a portion of the first connecting groove 340 means that part or all of the structure of the first connecting groove 340 can be provided on the stator 360. Similarly, the fact that the rotor 370 is provided with at least a portion of the second connecting groove 350 means that part or all of the structure of the second connecting groove 350 can be provided on the rotor 370. It can be understood that the first connecting groove 340 can be composed of different parts provided on the stator 360 and the rotor 370, and the second connecting groove 350 can be composed of different parts provided on the stator 360 and the rotor 370.

[0132] Please see Figure 2 , Figure 3 and Figure 4 In some embodiments, the first connecting slot 340 includes a first slot 341 and a second slot 342 that can be connected, and the first slot 341 and the second slot 342 are respectively disposed on the stator 360 and the rotor 370.

[0133] Thus, during the rotation of rotor 370, the second groove 342 can move to a position communicating with the first groove 341, allowing liquid to flow from the first groove 341 to the second groove 342.

[0134] Specifically, the first groove 341 can be recessed into the end face of the stator 360 along the axial direction of the stator 360, and the second groove 342 can be disposed on the rotor 370 with its opening facing the stator 360. The first groove 341 and the second groove 342 can be connected or separated during the rotation of the rotor 370. For example, when the rotor 370 rotates and the rotary valve 300 is in the first valve position, the first groove 341 and the second groove 342 are connected.

[0135] Please see Figure 2 , Figure 3 and Figure 4 In some embodiments, the first slot 341 has two ends, one end of the first slot 341 is connected to the first common port 310 and the other end is connected to the second slot 342. The second slot 342 has two ends, one end of the second slot 342 is connected to the first slot 341 and the other end is optionally connected to a connection port 330.

[0136] Thus, the first common port 310 can be connected to a connection port 330 through the first slot 341 and the second slot 342.

[0137] Specifically, for ease of functional implementation, both the first groove 341 and the second groove 342 can be arc-shaped grooves, or they can have other shapes. The embodiments of the present invention do not limit the specific shape or structure of the first groove 341 and the second groove 342. The first groove 341 may include a first end 3410 and a second end 3411. The first end 3410 may be located near the first common port 310, and the second end 3411 may be located away from the first common port 310.

[0138] Similarly, the second groove 342 may include a first end 3420 and a second end 3421 of the second groove 342. The second end 3421 of the second groove 342 is one end on the arc of the second groove 342 that is connected to a connection port 330. The second end 3421 of the second groove 342 may be set perpendicular to the arc of the second groove 342.

[0139] When the second common port 320 is blocked, the liquid flows into the rotary valve 300 through the first common port 310, and flows along the arc of the first groove 341 to the second end 3411 of the first groove 341. Then it flows from the second end 3411 of the first groove 341 to the first end 3420 of the second groove 342. Then it flows along the arc of the second groove 342 to the second end 3421 of the second groove 342. Finally, the liquid flows out of the stator 360.

[0140] It is worth noting that during the rotation of the rotor 370, the second end 3421 of the second slot 342 can be selectively connected to a connection port 330. That is, when the rotor 370 rotates to different positions, the second end 3421 of the second slot 342 is connected to a corresponding connection port 330.

[0141] Please see Figures 2 to 6 In some embodiments, the stator 360 includes a first end face 361 and a second end face 362 facing away from each other, and the rotor 370 includes a third end face 371 and a fourth end face 372 facing away from each other. The second end face 362 and the third end face 371 are in contact with each other. A first groove 341 is formed on the first end face 361, and a second groove 342 is formed on the third end face 371.

[0142] Thus, the fit between the second end face 362 and the third end face 371 can prevent liquid leakage. The first groove 341 and the second groove 342 are formed on the first end face 361 and the third end face 371, respectively. This makes the first connecting groove 340 easier to manufacture and can reduce the manufacturing cost of the stator 360 and the rotor 370.

[0143] Specifically, the first end face 361 is the end face where the liquid first flows into the stator 360. The first stator 360 and the rotor 370 are attached to each other through the second end face 362 and the third end face 371, so that after the liquid enters the stator 360 and rotor 370 of the rotary valve 300, it can only flow along the first groove 341 and the second groove 342. The first groove 341 is formed on the first end face 361, and the groove opening of the first groove 341 is set away from the third end face 371 of the rotor 370. The second groove 342 is formed on the third end face 371, and the groove opening of the second groove 342 faces the second end face 362 of the stator 360.

[0144] Please see Figure 2 , Figure 3 and Figure 5 In some embodiments, the second connecting groove 350 has two ends, one end of which is connected to the second common port 320 and the other end is optionally connected to a connection port 330.

[0145] Thus, the second connecting slot 350 allows the second common port 320 to be connected to a connecting port 330.

[0146] Specifically, the second connecting channel 350 can be a straight channel, and the second connecting channel 350 can be formed on the third end face 371. When the first common port 310 is blocked, the liquid flows into the rotary valve 300 through the second common port 320. Further, the liquid flows into the first end 351 of the second connecting channel 350 through the second common port 320, and flows into the second end 352 of the second connecting channel 350 in a straight line along the second connecting channel 350 via the first end 351, and finally flows into a connection port 330.

[0147] It is worth noting that during the rotation of the rotor 370, the second end 352 of the second connecting groove 350 can be selectively connected to a connection port 330. That is, when the rotor 370 rotates to different positions, the second end 352 of the second connecting groove 350 is connected to a corresponding connection port 330.

[0148] Please see Figures 2 to 6 In some embodiments, the stator 360 includes a first end face 361 and a second end face 362 facing away from each other, and the rotor 370 includes a third end face 371 and a fourth end face 372 facing away from each other. The second end face 362 and the third end face 371 are fitted together, and a second connecting groove 350 is formed on the third end face 371.

[0149] Thus, the fit between the second end face 362 and the third end face 371 can prevent liquid leakage, and the second connecting groove 350 formed on the third end face 371 can reduce the manufacturing cost of the stator 360 and the rotor 370.

[0150] Specifically, the second connecting groove 350 is recessed in the third end face 371 of the rotor 370, and the opening of the second connecting groove 350 faces the second end face 362 along the axial direction of the stator 360.

[0151] Please see Figure 1 In some embodiments, when the liquid system 1000 is in the first state, the pump assembly 400 also controls the liquid in the third flow path 12 to flow back to the second flow path 11 to clean the second flow path 11.

[0152] In this way, the reagents used for sequencing that remain in the second flow path 11 can be cleaned.

[0153] Specifically, when the liquid system 1000 is in the first state, the pump assembly 400 can create a negative pressure, driving the liquid in the first storage 100 to flow through the rotary valve 300 to the third flow path 12. Then, the pump assembly 400 can create a positive pressure, driving the liquid in the third flow path 12 to flow back to the rotary valve 300, and the liquid flows through the second common port 320 of the rotary valve 300 to the second flow path 11 and cleans the second flow path 11.

[0154] Please see Figure 7In some embodiments, the liquid circuit system 1000 includes a multi-way valve 15 disposed between the rotary valve 300 and the reaction device 500, which changes its own passage to put the liquid circuit system 1000 into a first state or a second state.

[0155] Thus, the liquid system 1000 can switch between the first and second states through the multi-way valve 15, thereby enabling the liquid system 1000 to perform cleaning and sequencing functions simultaneously.

[0156] Specifically, in the hydraulic system 1000 equipped with multi-way valves 15, the liquid outlet of the rotary valve 300 is the second common port 320. The number of multi-way valves 15 can correspond one-to-one with the number of rotary valves 300, independently controlling the liquid flowing through different rotary valves 300. The multi-way valves 15 can be connected to the third flow path 12 and the fourth flow path 13 respectively. The multi-way valves 15 change their own pathways to put the hydraulic system 1000 into a first state or a second state. For example, the multi-way valve 15 can close its connection to the third flow path 12, thereby putting the hydraulic system 1000 into the second state. As another example, the multi-way valve 15 can close its connection to the fourth flow path 13, thereby putting the hydraulic system 1000 into the first state.

[0157] Please see Figure 7 In some embodiments, the multi-port valve 15 includes a first port 150, a second port 151, and a third port 152. The first port 150 is selectively connected to either the second port 151 or the third port 152. The first port 150 is connected to the rotary valve 300. The second port 151 is connected to one end of the third flow path 12, and the other end of the third flow path 12 is connected to the pump assembly 400. The third port 152 is connected to one end of the fourth flow path 13, and the other end of the fourth flow path 13 is connected to the reaction device 500. When the first port 150 is connected to the second port 151, the liquid system 1000 is in a first state. When the first port 150 is connected to the third port 152, the liquid system 1000 is in a second state.

[0158] Thus, by connecting the first port 150 to the second port 151 and the third port 152 respectively, the liquid system 1000 can be in the first state and the second state respectively, so the liquid system 1000 can simultaneously realize the functions of cleaning and sequencing.

[0159] Specifically, the multi-port valve 15 can be a solenoid valve, which may include an electromagnet. The solenoid valve controls the connection between the first port 150 and the second port 151 and the third port 152 by controlling whether the electromagnet is energized. When the first port 150 and the second port 151 are connected, the first storage device 100 and the rotary valve 300 are connected to the pump assembly 400 through the third flow path 12. At this time, the liquid system 1000 is in the first state, and the cleaning solution in the first storage device 100 can enter the first flow path 10, the rotary valve 300, and the third flow path 12. When the first port 150 and the third port 152 are connected, the second storage device 200 and the rotary valve 300 are connected to the reaction device 500 through the fourth flow path 13. The sequencing reagents in the second storage device 200 can enter the reaction device 500 for sequencing reaction.

[0160] Please see Figure 7 In some embodiments, the rotary valve 300 includes a common port 301 and a plurality of connection ports 330. The common port 301 is selectively connected to at least one of the connection ports 330. The common port 301 is connected to a first port 150. The first flow path 10 and the second flow path 11 are respectively connected to the corresponding connection ports 330. When the rotary valve 300 is in the first valve position, the common port 301 is connected to the first flow path 10 through at least one of the connection ports 330. When the rotary valve 300 is in the second valve position, the common port 301 is connected to the second flow path 11 through the remaining connection ports 330.

[0161] Thus, when the rotary valve 300 is in different positions, the common port 301 can be connected to the first flow path 10 and the second flow path 11 through different connection ports 330, thereby simplifying the liquid circuit system 1000.

[0162] Specifically, by rotating the rotor 370 relative to the stator 360, the common port 301 can be connected to different connection ports 330, thereby connecting the first flow path 10 or the second flow path 11.

[0163] It should be pointed out that, Figure 7 Other parts of the hydraulic system 1000 not described in the implementation embodiment can be found in the following reference. Figure 1 The parts of the liquid circuit system 1000 in the embodiments that are the same as or similar to those in the embodiments will not be described again here.

[0164] Please see Figure 1 , Figures 7 to 10In some embodiments, the liquid circuit system 1000 includes a first reagent needle 16 and a first drive mechanism 17. The first reagent needle 16 is connected to a first flow path 10 and / or a second flow path 11. The first drive mechanism 17 is used to drive the first reagent needle 16 to move along the length direction of the first reagent needle 16 so that the first reagent needle 16 extends into the first memory 100 and / or the second memory 200.

[0165] Thus, driven by the first driving mechanism 17, the first reagent needle 16 can be inserted into the first memory 100 and / or the second memory 200 to extract liquid from the first memory 100 and / or the second memory 200.

[0166] It should be noted that when there is only one first reagent needle 16, the first reagent needle 16 can be inserted into the first memory 100 or the second memory 200 each time; when there are multiple first reagent needles 16, the multiple first reagent needles 16 can be inserted into the first memory 100 and the second memory 200 respectively each time, or the multiple first reagent needles 16 can all be inserted into one of the first memory 100 and the second memory 200 each time.

[0167] Specifically, the first reagent needle 16 can be used to draw reaction solution, biological sample liquid, or other liquids. After the liquid enters the first reagent needle 16, under the negative pressure provided by the pump assembly 400, it can flow into the reaction device 500 through the rotary valve 300 to carry out the corresponding reaction. The rotary valve 300 can select different liquids to flow into the reaction device 500 in sequence to complete the corresponding reaction.

[0168] The first reagent needle 16 includes a first reagent needle 160, a second reagent needle 161, and a third reagent needle 162. The number of the first reagent needle 160, the second reagent needle 161, and the third reagent needle 162 is unlimited, and they are used to draw the corresponding liquid.

[0169] The first reagent needle 160 is connected to the rotary valve 300 of the first liquid circuit unit 600 via a pipeline, so that the liquid drawn by the first reagent needle 160 enters the first unit 501 of the reaction device 500 through the rotary valve 300 of the first liquid circuit unit 600; the second reagent needle 161 is connected to the rotary valve 300 of the second liquid circuit unit 700 via a pipeline, so that the liquid drawn by the second reagent needle 161 enters the second unit 502 of the reaction device 500 through the rotary valve 300 of the second liquid circuit unit 700; the third reagent needle 162 enters the reaction device 500 through the third manifold 18 (e.g., ...). Figure 1(As shown) is connected to the rotary valves 300 of the first liquid circuit unit 600 and the second liquid circuit unit 700 respectively, so that the liquid drawn by the third reagent needle 162 enters the first unit 501 and the second unit 502 of the reaction device 500 simultaneously through the rotary valves 300 of the first liquid circuit unit 600 and the second liquid circuit unit 700 respectively.

[0170] The third manifold 18 has multiple Y-shaped channels arranged side by side along its length. Each Y-shaped channel can divide the liquid flowing into the third manifold 18 into two. Each Y-shaped channel includes one inlet and two outlets. The inlet of the Y-shaped channel is connected to the third reagent needle 162 via a pipeline, and the two outlets of the Y-shaped channel are connected to the rotary valve 300 of the first liquid path unit 600 and the rotary valve 300 of the second liquid path unit 700 via pipelines, respectively.

[0171] The liquid drawn by the third reagent needle 162 is divided into two by the third manifold 18, and can flow into the first unit 501 and the second unit 502 of the reaction device 500 through the rotary valve 300 of the first liquid circuit unit 600 and the rotary valve 300 of the second liquid circuit unit 700, respectively. This enables the liquid circuit system 1000 to simultaneously supply the same liquid to the first unit 501 and the second unit 502 of the reaction device 500, thereby improving the liquid supply efficiency.

[0172] Any one of the first reagent needles 160 and any one of the second reagent needles 161 can be combined into a dual-needle configuration and inserted into the same memory to simultaneously or sequentially extract sequencing reagents stored in the memory, thereby supplying liquid to the first liquid circuit unit 600 and the second liquid circuit unit 700 respectively. This enables the first unit 501 and the second unit 502 to perform the same reaction, different reactions, or different steps of the same reaction, while reducing the number of memories storing the same type of liquid. As a result, the liquid circuit system 1000 has a more compact structure and smaller size.

[0173] The first memory 100 and / or the second memory 200 have multiple extraction ports 19 on their surfaces near the first reagent needle 16. The first reagent needle 16 extends into the first memory 100 and / or the second memory 200 through the extraction ports 19. The first reagent needle 16 can be used to extract liquid stored in the first memory 100 and the second memory 200. The first reagent needle 16 is fluidly connected to the first flow path 10 and / or the second flow path 11. The first reagent needle 16 can be fixed to the first drive mechanism 17, and the fixing method can be a threaded connection or the like.

[0174] Please see Figure 10In some embodiments, the first drive mechanism 17 includes a fixed frame 170, a movable member 171, and a first drive assembly 172. The movable member 171 is movably disposed on the fixed frame 170. The first drive assembly 172 is disposed on the fixed frame 170, and the first reagent needle 16 is mounted on the movable member 171; the first drive assembly 172 is used to drive the movable member 171 to move relative to the fixed frame 170.

[0175] Thus, the first reagent needle 16 mounted on the movable part 171 can move relative to the fixed frame 170 via the first drive assembly 172.

[0176] Specifically, the mounting bracket 170 can be positioned above the first memory 100 and the second memory 200, and the first reagent needle 16 can be fixed to the mounting bracket 170. Further, the first reagent needle 16 can be fixed to the movable member 171. The first driving assembly 172 drives the movable member 171 to move relative to the mounting bracket 170, thereby causing the first reagent needle 16 to move along its length.

[0177] Please see Figure 10 In some embodiments, the fixing frame 170 includes a first plate 173, a second plate 174 and a connecting post 175. The first plate 173 and the second plate 174 are spaced apart. The connecting post 175 connects the first plate 173 and the second plate 174. The movable member 171 is movably sleeved on the connecting post 175. The first drive assembly 172 is mounted on the first plate 173 and the second plate 174.

[0178] Thus, the first drive assembly 172 can drive the movable part 171 sleeved on the connecting post 175 to move, thereby driving the first reagent needle 16 fixedly connected to the movable part 171 to move along the length direction of the first reagent needle 16.

[0179] Specifically, the first reagent needle 16 can be inserted through the first plate 173, the second plate 174, and the movable member 171, and is fixedly connected to the movable member 171. The first plate 173, the second plate 174, and the movable member 171 can be arranged in an upper, middle, and lower position. The first plate 173 and the second plate 174 are respectively connected to the two ends of the connecting post 175 and fixedly connected to the connecting post 175. The movable member 171 can move between the first plate 173 and the second plate 174 along the axial direction of the connecting post 175. The axial direction of the connecting post 175 is parallel to the length direction of the first reagent needle 16.

[0180] Please see Figure 10 In some embodiments, the first drive assembly 172 includes a first motor 1720 and a first lead screw 1721 connected to the first motor 1720. A movable member 171 is sleeved on the first lead screw 1721, and the first motor 1720 drives the movable member 171 to move through the first lead screw 1721.

[0181] Thus, the first motor 1720 drives the movable part 171 through the first lead screw 1721, so that the movable part 171 has good positional accuracy and repeatability of motion.

[0182] Specifically, the first motor 1720 can be fixedly connected to the first plate 173. One end of the first lead screw 1721 is connected to the first motor 1720 and passes through the first plate 173. The movable part 171 is sleeved on the first lead screw 1721 and fixedly connected to the first lead screw 1721. When the motor moves, it drives the first lead screw 1721 to move along the axial direction of the first lead screw 1721, thereby causing the first lead screw 1721 to drive the movable part 171 to move, and thus causing the first reagent needle 16 fixed on the movable part 171 to move with the movable part 171. The axial direction of the lead screw is parallel to the length direction of the first reagent needle 16.

[0183] Please see Figure 1 , Figure 7 and Figure 10 In some embodiments, the liquid circuit system 1000 includes a second reagent needle 20 and a mounting base 21. The second reagent needle 20 is shorter than the first reagent needle 16. The mounting base 21 is mounted on a fixing frame 170. The second reagent needle 20 is connected to the first flow path 10 and / or the second flow path 11. When the movable member 171 moves toward the first memory 100 and / or the second memory 200, it causes the second reagent needle 20 to extend into the first memory 100 and / or the second memory 200.

[0184] Thus, the liquid system 1000 can be adapted to reagent needles of different sizes, and the liquid system 1000 has good versatility. The second reagent needle 20 can be used to extract biological sample solutions.

[0185] Specifically, the second reagent needle 20 is mounted on the second plate 174 of the fixing frame 170 by means of bolt connection or other means. One end of the second reagent needle 20 is fluidly connected to the first flow path 10 and / or the second flow path 11. The second reagent needle 20 is mounted on the mounting base 21 and passes through the second plate 174. The second reagent needle 20 is fixedly connected to the movable member 171. When the movable member 171 moves toward the first memory 100 and / or the second memory 200, it can drive the second reagent needle 20 to extend into the first memory 100 and / or the second memory 200.

[0186] Please see Figure 1 , Figure 7 , Figures 10 to 12In some embodiments, the mounting base 21 includes a guide 210 and a slider 211. The guide 210 is mounted on the fixed frame 170, and the slider 211 is movably disposed on the guide 210. The second reagent needle 20 is mounted on the slider 211. When the movable member 171 moves toward the first memory 100 and / or the second memory 200, it drives the slider 211 to move.

[0187] Thus, when the movable part 171 moves toward the first memory 100 and / or the second memory 200, it can drive the sliding part 211 to move, thereby driving the second reagent needle 20 mounted on the sliding part 211 to move.

[0188] Specifically, the guide member 210 can be fixedly installed on the fixed frame 170 by means of bolt connection or other means. The guide member 210 can have a slot, which extends along the axial direction of the guide member 210. The sliding member 211 can be set in the slot, and the groove wall is used to guide the sliding member 211 so that the sliding member 211 will not shake when it moves.

[0189] Please see Figure 1 , Figure 7 , Figures 10 to 12 In some embodiments, a first elastic member 22 is provided between the sliding member 211 and the fixed frame 170. The first elastic member 22 compresses and stores force when the movable member 171 moves toward the first memory 100 and / or the second memory 200, and releases the stored force after the force between the movable member 171 and the sliding member 211 is eliminated, so that the second reagent needle 20 moves out of the first memory 100 and / or the second memory 200.

[0190] Thus, the first elastic element 22 can cause the second reagent needle 20 to move out of the first memory 100 and / or the second memory 200, thereby causing the second reagent needle 20 to leave the first memory 100 and / or the second memory 200, and avoiding interference between the process of the second reagent needle 20 moving laterally relative to the memory and the memory.

[0191] Specifically, the movable member 171 may include a lever 1710. When the movable member 171 moves toward the first memory 100, the lever 1710 can press against the sliding member 211, thereby causing the sliding member 211 to move toward the first memory 100 and / or the second memory 200, thus driving the second reagent needle 20 to move toward the first memory 100 and / or the second memory 200. When the second reagent needle 20 abuts against the bottom of the memory, the force between the movable member 171 and the sliding member 211 is eliminated, and the stored force of the first elastic member 22 is released, thereby allowing the second reagent needle 20 to move out of the first memory 100 and / or the second memory 200. When the force between the first elastic member 22 and the second reagent needle 20 is balanced, the movement of the second reagent needle 20 toward the first memory 100 and / or the second memory 200 is limited.

[0192] Please see Figure 1 , Figure 7 , Figures 10 to 12 In some embodiments, a second elastic member 23 is connected between the slider 211 and the second reagent needle 20. The second elastic member 23 applies a force in a first direction to the second reagent needle 20 to keep the second reagent needle 20 on the slider 211. When the second reagent needle 20 is subjected to a force in a second direction, the second elastic member 23 is compressed under the action of the second reagent needle 20. The first direction is the direction in which the second reagent needle 20 is directed toward the first memory 100 and / or the second memory 200, and the second direction is opposite to the first direction.

[0193] Thus, the second elastic member 23 can keep the second reagent needle 20 on the slider 211 and can counteract the force formed after the second reagent needle 20 contacts the bottom of the memory, preventing the second reagent needle 20 from puncturing the memory or bending the second reagent needle 20.

[0194] Specifically, when the lever 1710 presses against the slider 211, the second elastic member 23 applies a force to the second reagent needle 20 toward the first memory 100 and / or the second memory 200, causing the second reagent needle 20 to move in the first direction along with the slider 211. When the second reagent needle 20 contacts the bottom of the memory, the bottom of the memory provides an upward force to the second reagent needle 20. Under the combined action of this force and the downward force of the lever 1710, the second elastic member 23 is compressed. In this way, the interaction force between the second reagent needle 20 and the memory is kept within a certain range, preventing the second reagent needle 20 from puncturing the memory or bending the second reagent needle 20.

[0195] Please see Figure 1 , Figure 7 , Figures 10 to 12In some embodiments, the slider 211 is provided with a mounting hole 2110, the second reagent needle 20 includes a needle body 201 and a flange 202 disposed on the needle body 201, the needle body 201 passes through the slider 211, the flange 202 is disposed in the mounting hole 2110, and the second elastic member 23 abuts against the flange 202.

[0196] Thus, the second elastic element 23 applies a force to the second reagent needle 20 by abutting against the flange 202, thereby restricting the movement of the second reagent needle 20 away from the first memory 100 and / or the second memory 200.

[0197] Specifically, the mounting hole 2110 can be a regular shape such as round or square, or it can be an irregular shape. The needle body 201 can be used to draw liquid from the first memory 100 and / or the second memory 200. The flange 202 can be arranged circumferentially around the needle body 201, and the radial dimension of the flange 202 is smaller than the diameter of the mounting hole 2110. When the sliding member 211 moves toward the first memory 100 and / or the second memory 200, the second elastic member 23 abuts against the flange 202, thereby driving the needle body 201 to move toward the first memory 100 and / or the second memory 200.

[0198] Please see Figure 1 , Figure 7 , Figure 10 In some embodiments, the fluid system 1000 includes a first detection component 24 disposed on a fixture 170, the first detection component 24 being used to detect the position of the movable part 171.

[0199] In this way, the position of the movable part 171 can be detected, the stroke of the movable part 171 is fixed, and the movable part 171 will not interfere with the first plate 173 or the second plate 174 during movement; in addition, by detecting the position of the movable part 171, the position of the reagent needle inserted into or out of the memory can be determined, thus preventing the reagent needle from moving laterally relative to the memory when it is inserted into the memory.

[0200] Specifically, the first detection component 24 can be fixedly connected to the fixing frame 170, or in other words, the first detection component 24 and the fixing frame 170 maintain a constant relative position.

[0201] Please see Figure 1 , Figure 7 , Figure 10 In some embodiments, the first detection component 24 includes a first photoelectric switch 240 and a second photoelectric switch 241 arranged at intervals along the movement direction of the movable member 171. The first photoelectric switch 240 and the second photoelectric switch 241 are used to detect the upper limit position and the lower limit position of the movable member 171, respectively.

[0202] Therefore, compared with other detection components, photoelectric switches have strong anti-interference capabilities and high detection reliability.

[0203] Specifically, the mounting bracket 170 may include a support plate 176, and the first photoelectric switch 240 and the second photoelectric switch 241 may be arranged at intervals on the support plate 176 along the movement direction of the movable member 171. A light-shielding member 1711 is provided on the movable member 171, and the light-shielding member 1711 may cooperate with the first photoelectric switch 240 or the second photoelectric switch 241 to detect the upper limit position and the lower limit position of the movable member 171.

[0204] For example, when the movable element 171 moves toward the first memory 100 and / or the second memory 200 to allow the first reagent needle 16 and the second reagent needle 20 to draw liquid, if the light-shielding element 1711 blocks the second photoelectric switch 241, the movable element 171 moves to its lower limit position. As another example, when the movable element 171 moves away from the first memory 100 and / or the second memory 200, if the light-shielding element 1711 blocks the first photoelectric switch 240, the movable element 171 moves to its upper limit position. The lower limit position is the position where the movable element 171 is closest to the first memory 100 and / or the second memory 200, and the upper limit position is the position where the movable element 171 is farthest from the first memory 100 and / or the second memory 200.

[0205] Please see Figure 1 , Figure 7 , Figure 13 In some embodiments, the liquid system 1000 includes a liquid collector 25 for collecting liquids generated by the liquid system 1000. The liquid collector 25 is provided with a liquid inlet 250. The liquid system 1000 also includes a cover assembly 26. During the process of driving the first reagent needle 16 to extend into the first memory 100 and / or the second memory 200, the first drive mechanism 17 drives the cover assembly 26 to close and press the liquid inlet 250. Alternatively, during the process of driving the first reagent needle 16 to leave the first memory 100 and / or the second memory 200, the first drive mechanism 17 drives the cover assembly 26 to open the liquid inlet 250.

[0206] Thus, the process of the cap assembly 26 closing the inlet 250 is synchronized with the process of the first reagent needle 16 extending into the first memory 100 and / or the second memory 200, and the process of the cap assembly 26 opening the inlet 250 is synchronized with the process of the first reagent needle 16 leaving the first memory 100 and / or the second memory 200. This allows the cap assembly 26 to automatically close the inlet 250 when the liquid system 1000 generates liquid, and to open the inlet 250 when the liquid system 1000 does not generate liquid, thereby making the liquid system 1000 convenient to use.

[0207] Specifically, the liquid generated by the liquid system 1000 flows through the drain pipe 71 into the liquid collector 25 located downstream of the pump assembly 400. A liquid manifold 70 is also located upstream of the liquid collector 25, which collects the liquid generated in the liquid system 1000 and inputs it into the liquid collector 25. The first drive mechanism 17 can simultaneously drive the first reagent needle 16 and the cap assembly 26, so that the first reagent needle 16 and the cap assembly 26 are linked together.

[0208] Please see Figure 1 , Figure 7 and Figure 13 In some embodiments, the first drive mechanism 17 includes a fixed frame 170, a movable member 171 movably disposed on the fixed frame 170, and a first drive assembly 172 disposed on the fixed frame 170. The first reagent needle 16 is mounted on the movable member 171; the first drive assembly 172 is used to drive the movable member 171 to move relative to the fixed frame 170. During the movement of the movable member 171, the movable member 171 can drive the cap assembly 26 to close and press against the liquid inlet 250 or open the liquid inlet 250.

[0209] Thus, the first reagent needle 16 is mounted on the movable part 171, which in turn drives the cap assembly 26 to move. This allows the first reagent needle 16 to be linked with the cap assembly 26, thereby simplifying the structure and control logic and reducing the failure rate and cost of the first drive mechanism 17.

[0210] Specifically, the first reagent needle 16 can be fixedly connected to the movable component 171. The first driving component 172 can drive the movable component 171 to move toward or away from the first memory 100 and / or the second memory 200, thereby causing the first reagent needle 16 mounted on the movable component 171 to move toward or away from the first memory 100 and / or the second memory 200.

[0211] The first reagent needle 16 can be fixedly connected to the movable part 171. For example, the first reagent needle 16 can pass through the movable part 171, and a metal plate can be interference-fitted to the circumference of the first reagent needle 16. Both the metal plate and the movable part 171 can be provided with bolt holes, and the metal plate and the movable part 171 can be fixedly connected together by bolts, thereby making the movable part 171 indirectly connected to the first reagent needle 16. Since there can be multiple first reagent needles 16, setting the movable part 171 and the first reagent needle 16 to be indirectly connected allows for the independent assembly and disassembly of different first reagent needles 16.

[0212] Please see Figure 1 , Figure 7 and Figure 13In some embodiments, the cover assembly 26 includes a guide rail 260, a connecting frame 261, and a pressure cap 262. The guide rail 260 is mounted on the fixed frame 170, the connecting frame 261 is movably disposed on the guide rail 260, and the pressure cap 262 is disposed on the connecting frame 261. During the movement of the movable member 171, the movable member 171 can drive the connecting frame 261 to make the pressure cap 262 close and press against the liquid inlet 250 or open the liquid inlet 250.

[0213] Thus, by connecting the bracket 261 to drive the movable part 171, the pressure cap 262 can close and press the liquid inlet 250 or open the liquid inlet 250, so that the liquid generated by the liquid circuit system 1000 will not leak out, evaporate, or generate aerosol, and the liquid collector 25 can be removed when the liquid circuit system 1000 is not generating liquid.

[0214] Specifically, the guide rail 260 can be mounted on the fixed frame 170 by means of bolts or other methods. The connecting frame 261 can move on the guide rail 260, and the pressure cap 262 can be mounted on the connecting frame 261 by means of bolts or other methods. When the connecting frame 261 moves, it can drive the pressure cap 262 to move. The connecting frame 261 is fixedly connected to the movable part 171 by means of bolts or other methods. Therefore, when the movable part 171 moves, it can drive the connecting frame 261 to move, thereby driving the pressure cap 262 to move, which in turn causes the pressure cap 262 to close and press against the liquid inlet 250 or open the liquid inlet 250.

[0215] Please see Figure 1 , Figure 7 , Figure 14 and Figure 15 In some embodiments, the connecting frame 261 is provided with a first limiting position 2610 and a second limiting position 2611. The first limiting position 2610 and the second limiting position 2611 are spaced apart along the movement direction of the connecting frame 261. When the movable member 171 moves in the first direction, the movable member 171 can press against the first limiting position 2610 to close the pressure cap 262 and press the liquid inlet 250. When the movable member 171 moves in the second direction, the movable member 171 can abut against the second limiting position 2611 to open the pressure cap 262 and the liquid inlet 250. The first direction is the direction in which the first reagent needle 16 faces the first memory 100 and / or the second memory 200. The second direction is opposite to the first direction.

[0216] Thus, through the cooperation of the movable part 171 with the first limiting position 2610 and the second limiting position 2611, the movable part 171 can control the cap 262 to close and press the liquid inlet 250 or open the liquid inlet 250, thereby enabling the liquid inlet 250 to be automatically opened or closed, thereby improving the ease of use of the liquid circuit system 1000.

[0217] Specifically, the direction of movement of the connecting frame 261 is parallel to the direction of movement of the first reagent needle 16 toward the first memory 100 and / or the second memory 200.

[0218] Please see Figure 1 , Figure 7 , Figure 14 and Figure 15 In some embodiments, the connecting frame 261 is provided with a groove 2613, which extends along the movement direction of the connecting frame 261. The two groove walls of the groove 2613 along the movement direction of the connecting frame 261 respectively form a first limiting position 2610 and a second limiting position 2611.

[0219] Thus, the first limiting position 2610 and the second limiting position 2611 formed by the groove 2613 make the movement stroke of the connecting frame 261 consistent with the movement stroke of the movable part 171, thereby realizing the synchronization of the first reagent needle 16 extending into the first memory 100 and / or the second memory 200 with the cap 262 closing the liquid inlet 250.

[0220] Please see Figure 14 and Figure 15 In some embodiments, an elastic element 27 is provided between the connecting frame 261 and the pressure cap 262, with the two ends of the elastic element 27 connected to the connecting frame 261 and the pressure cap 262 respectively.

[0221] Thus, by connecting the connecting bracket 261 and the pressure cap 262 with the elastic element 27, the precision requirements for the pressure cap 262 to close and tighten the liquid inlet 250 can be reduced, ensuring that the pressure cap 262 reliably fits the liquid inlet 250. It is understandable that without the elastic element 27, when the connecting bracket 261 drives the pressure cap 262 to close the liquid inlet 250, there may be a certain gap, which would cause the pressure cap 262 to not completely tighten the liquid inlet 250, resulting in liquid leakage through the liquid inlet 250.

[0222] Specifically, the elastic element 27 can be a standard part such as a compression spring, and the embodiments of the present invention do not limit this.

[0223] Please see Figure 1 , Figure 7 , Figure 13 , Figure 16 In some embodiments, the first memory 100 and the second memory 200 are both located in the same kit 60, and the liquid collector 25 and the kit 60 are both disposed on the same pull-out device 28, which is used to be disposed in the housing of the liquid circuit system 1000 by pulling out.

[0224] Thus, the liquid collector 25 and reagent kit 60 are easy to place and remove by pulling them out. This design makes the internal structure of the liquid circuit system 1000 compact, which is convenient for management and maintenance.

[0225] Specifically, pull-out devices 28 may be spaced out within the housing of the liquid system 1000. Each pull-out device 28 may include a drawer box 280 and a slide rail 281. The drawer box 280 is slidably mounted on the slide rail 281, which is located on both sides of the drawer box 280. The drawer box 280 may include a partition 2801, a reagent kit misplacement prevention block 2802, and a liquid collector misplacement prevention block 2803. The partition 2801 isolates the liquid collector 25 and the reagent kit 60; the reagent kit misplacement prevention block 2802 prevents the reagent kit 60 from being placed in the wrong position; and the liquid collector misplacement prevention block 2803 prevents the liquid collector 25 from being placed in the wrong position.

[0226] Please see Figure 16 In some embodiments, reagent kit 60 is kept at room temperature. Thus, the reagents in reagent kit 60 are stored according to storage conditions to prevent sample damage.

[0227] Please see Figure 1 , Figure 7 , Figure 9 and Figure 17 In some embodiments, the fluid system 1000 further includes a bracket 29 and a second drive mechanism 30 connected to the bracket 29. The first memory 100 and the second memory 200 are both mounted on the bracket 29. The second drive mechanism 30 is used to drive the bracket 29 to move so as to move the first memory 100 and the second memory 200.

[0228] Thus, the movement of the first memory 100 and the second memory 200 will change the position of the first reagent needle 16 relative to the first memory 100 and the second memory 200, thereby allowing the first reagent needle 16 to extract reagents stored in different locations in the first memory 100 and the second memory 200.

[0229] Specifically, the second drive mechanism 30 can be fixedly connected to the bracket 29. The first memory 100 and the second memory 200 can be detachably mounted on the bracket 29. The second drive mechanism 30 can drive the bracket 29 to move in a plane perpendicular to the direction of movement of the first reagent needle 16, thereby causing the first memory 100 and the second memory 200 to move in a plane perpendicular to the direction of movement of the first reagent needle 16.

[0230] Please see Figure 9 and Figure 17 In some embodiments, the second drive mechanism 30 includes a second motor 31 and a second lead screw 32 connected to the second motor 31. The second lead screw 32 is connected to a bracket 29, and the second motor 31 drives the bracket 29 to move through the second lead screw 32.

[0231] Thus, the second motor 31 drives the bracket 29 to move through the second lead screw 32, so that the bracket 29 has good positional accuracy and repeatability of movement.

[0232] Specifically, there can be multiple second motors 31 and second lead screws 32, with each second motor 31 corresponding to a second lead screw 32. Multiple second motors 31 can be spaced apart on both sides of the bracket 29. The bracket 29 can include a connecting rod, which can be located at the end of the bracket 29. The second lead screw 32 can be fixedly connected to the connecting rod, and its movement drives the bracket 29 to move.

[0233] Please see Figure 1 , Figure 7 , Figure 9 and Figure 17 In some embodiments, the fluid system 1000 includes a second detection component 40 for detecting the position of the bracket 29 to determine the positions of the first memory 100 and the second memory 200.

[0234] Thus, the position confirmation of the first memory 100 and the second memory 200 can ensure that the first reagent needle 16 can be inserted into the designated position in the first memory 100 and the second memory 200, thereby ensuring that the first reagent needle 16 can accurately extract the designated reagent.

[0235] Specifically, along the height direction of the first memory 100 and the second memory 200, the second detection component 40 can be disposed on the side of the first memory 100 and the second memory 200 near the first reagent needle 16.

[0236] Please see Figure 9 , Figure 17 and Figure 18 In some embodiments, the first memory 100 and the second memory 200 are both located in the same reagent kit 60. The second detection component 40 includes a third photoelectric switch 41, a fourth photoelectric switch 42, a first light-blocking element 43, and a second light-blocking element 44. The third photoelectric switch 41 and the fourth photoelectric switch 42 are spaced apart above the bracket 29 along the movement direction of the bracket 29. The first light-blocking element 43 and the second light-blocking element 44 can both abut against the reagent kit 60. The first light-blocking element 43 and the second light-blocking element 44 can both move in a direction perpendicular to the movement of the reagent kit 60 when the reagent kit 60 moves. When the reagent kit 60 is in the first position, the first light-blocking element 43 blocks the light path of the third photoelectric switch 41. When the reagent kit 60 is in the second position, the second light-blocking element 44 blocks the light path of the fourth photoelectric switch 42.

[0237] Thus, by determining whether the light paths of the third photoelectric switch 41 and the fourth photoelectric switch 42 are blocked, the location of the reagent kit 60 can be determined.

[0238] Specifically, the parts of the first light-blocking element 43 and the second light-blocking element 44 that contact the reagent kit 60 can be designed as inclined surfaces. The inclined surfaces have a certain angle with the reagent kit 60, and when they contact the reagent kit 60, they move in a direction perpendicular to the movement of the reagent kit 60. Taking the liquid drawn by the first reagent needle 16 as sequencing reagent and washing solution as an example: When the carrier 29 moves the reagent kit 60 to the first position, the first light-blocking element 43 moves in a direction perpendicular to the movement of the reagent kit 60 until the light path of the third photoelectric switch 41 is blocked by the first light-blocking element 43. At this time, it is determined that the first reagent needle 16 is aligned with the second memory 200. When the carrier 29 continues to move the reagent kit 60 to the second position, the second light-blocking element 44 moves in a direction perpendicular to the movement of the reagent kit 60 until the light path of the fourth photoelectric switch 42 is blocked by the second light-blocking element 44. At this time, it is determined that the first reagent needle 16 is aligned with the first memory 100.

[0239] Please see Figure 9 and Figure 17 In some embodiments, the first memory 100 and the second memory 200 are both located in the same kit 60. The second detection component 40 includes a fifth photoelectric switch 45 and a third light-blocking element 46. The third light-blocking element 46 is disposed on the bracket 29. When the bracket 29 is in the initial position, the third light-blocking element 46 blocks the light path of the fifth photoelectric switch 45.

[0240] Thus, the fifth photoelectric switch 45 and the third light-blocking element 46 work together to determine whether the reagent kit 60 is placed in the designated position.

[0241] Specifically, when the third light-blocking element 46 blocks the light path of the fifth photoelectric switch 45, the reagent kit 60 is placed in a designated position. The designated position refers to the initial position. By changing the position of the bracket 29, the reagent kit 60 can move from the initial position to a first position or a second position. In some embodiments, the designated position is the first position of the reagent kit 60, and by changing the position of the bracket 29, the reagent kit 60 can move from the first position to the second position.

[0242] Please see Figure 9 and Figure 19 In some embodiments, the first memory 100 and the second memory 200 are both located in the same reagent kit 60. The second detection component 40 includes a third photoelectric switch 41, a fourth photoelectric switch 42 and a first light-blocking element 43. The third photoelectric switch 41 and the fourth photoelectric switch 42 are spaced apart on the side of the bracket 29 along the movement direction of the bracket 29. The first light-blocking element 43 is disposed on the bracket 29. When the reagent kit 60 is in the first position, the first light-blocking element 43 blocks the light path of the third photoelectric switch 41. When the reagent kit 60 is in the second position, the first light-blocking element 43 blocks the light path of the fourth photoelectric switch 42.

[0243] Thus, by determining whether the light paths of the third photoelectric switch 41 and the fourth photoelectric switch 42 are blocked, the location of the reagent kit 60 can be determined.

[0244] Specifically, the first light-blocking element 43 blocks the light path of the third photoelectric switch 41 when the reagent kit 60 is in the first position, at which time the reagent kit 60 is in the first position. The first light-blocking element 43 blocks the light path of the fourth photoelectric switch 42 when the reagent kit 60 is in the second position, at which time the reagent kit 60 is in the second position.

[0245] Please see Figure 9 and Figure 19 In some embodiments, the first memory 100 and the second memory 200 are both located in the same reagent kit 60. The second detection component 40 also includes a fifth photoelectric switch 45 and a third light-blocking component 46. The third light-blocking component 46 can move in a direction perpendicular to the movement of the reagent kit 60 during the process of placing the reagent kit 60 into the tray 29, so as to block the light path of the fifth photoelectric switch 45.

[0246] Thus, the fifth photoelectric switch 45 and the third light-blocking element 46 work together to determine whether the reagent kit 60 is placed in the designated position.

[0247] Specifically, when the third light-blocking element 46 blocks the light path of the fifth photoelectric switch 45, the reagent kit 60 is placed in a designated position. The designated position refers to the initial position. By changing the position of the bracket 29, the reagent kit 60 can move from the initial position to a first position or a second position. In some embodiments, the designated position is the first position of the reagent kit 60, and by changing the position of the bracket 29, the reagent kit 60 can move from the first position to the second position.

[0248] Please see Figure 9 and Figure 20 In some embodiments, the bracket 29 is provided with a limiting structure that restricts the movement of the first memory 100 and the second memory 200 relative to the bracket 29.

[0249] In this way, the first memory 100 and the second memory 200 can be positioned so that the first reagent needle 16 can be accurately inserted into the first memory 100 and the second memory 200.

[0250] Specifically, the bracket 29 may include a first limiting block 290, which may be disposed on both sides of the bracket 29 along its width direction. The first limiting block 290 is used to limit the movement of the first memory 100 and the second memory 200 along the height direction of the bracket 29, thereby improving the service life of the first memory 100 and the second memory 200. The bracket 29 may also include a second limiting block 291, which is used to limit the displacement of the first memory 100 and the second memory 200 along the length direction of the bracket 29. The second limiting block 291 may be disposed at both ends of the bracket 29 along its length direction.

[0251] Please see Figure 8 and Figure 9 In some embodiments, the fluid system 1000 includes a cooling device 50, and a bracket 29 houses the first memory 100 and the second memory 200 within the cooling device 50.

[0252] In this way, the cooling device 50 can adjust the temperature of the first memory 100 and the second memory 200 to a suitable temperature for storing reagents, thus ensuring the availability of the reagents.

[0253] Specifically, the cooling device 50 may include a housing 51, which may include a chamber 510. The bracket 29 may be fixed in the chamber 510, and the first memory 100 and the second memory 200 may be housed in the chamber 510.

[0254] Please see Figure 8 , Figure 9 , Figure 20 and Figure 21 In some embodiments, the bottom of the bracket 29 is provided with a through hole 292 through the bracket 29.

[0255] Thus, the through hole 292 allows the condensate generated by the cooling device 50 cooling the first memory 100 and the second memory 200 to flow to the outside of the bracket 29, preventing condensate accumulation and extending the service life of the first memory 100 and the second memory 200. The through hole 292 can also reduce the weight of the bracket 29 and improve its air permeability.

[0256] Specifically, the through hole 292 can be a regular shape such as an oblong hole or a circular hole, or it can be an irregular shape. The cooling device 50 may also include a lower plate 52, which can be positioned below the bracket 29 along the height direction of the cooling device 50, allowing condensate in the through hole 292 to flow to the lower plate 52. The lower plate 52 may include a receiving groove 520, which can be configured with a structure that is higher on both sides and lower in the middle. The side wall of the receiving groove 520 has a water outlet 521. The lower plate 52 may also include a strip groove 523, which can be multiple. The multiple strip grooves 523 are arranged along the length direction of the lower plate 52, and the strip grooves 523 are inclined towards the receiving groove 520. The strip grooves 523 are used to guide the condensate flowing from the through hole 292 to the lower plate 52, so that the condensate is guided into the receiving groove 520 and discharged through the water outlet 521.

[0257] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0258] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A fluid circuit system, characterized in that, include: Independent first and second memory; A rotary valve, wherein the first memory is connected to the rotary valve via a first flow path, and the second memory is connected to the rotary valve via a second flow path; A pump assembly, which is fluidly connected to the rotary valve via a third flow path; A reaction apparatus, wherein the reaction apparatus is fluidly connected to the rotary valve via a fourth flow path; The liquid circuit system is selectively in a first state or a second state. When the liquid circuit system is in the first state, the first memory is connected to the pump assembly through the first flow path, the rotary valve and the third flow path. The pump assembly is used to drive the liquid in the first memory into the third flow path. When the liquid circuit system is in the second state, the second storage device is connected to the reaction device through the second flow path, the rotary valve and the fourth flow path, and the pump assembly is used to drive the liquid in the second storage device into the reaction device; The liquid circuit system includes a first reagent needle and a first driving mechanism. The first reagent needle is connected to the first flow path and / or the second flow path. The first driving mechanism is used to drive the first reagent needle to move along the length direction of the first reagent needle so that the first reagent needle extends into the first memory and / or the second memory. The liquid system includes a liquid collector for collecting liquids generated by the system, the liquid collector having an inlet, and a cover assembly. During the process of driving the first reagent needle to extend into the first memory and / or the second memory, the first driving mechanism drives the cover assembly to close and press against the liquid inlet. or, During the process of driving the first reagent needle away from the first memory and / or the second memory, the first driving mechanism drives the cover assembly to open the liquid inlet.

2. The fluid circuit system according to claim 1, characterized in that, The liquid circuit system includes a multi-way valve disposed between the rotary valve and the reaction device, the multi-way valve changing its own passage to allow the liquid circuit system to be in the first state or the second state.

3. The fluid circuit system according to claim 2, characterized in that, The multi-port valve includes a first port, a second port, and a third port. The first port is selectively connected to either the second port or the third port. The first port is connected to the rotary valve. The second port is connected to one end of the third flow path, and the other end of the third flow path is connected to the pump assembly. The third port is connected to one end of the fourth flow path, and the other end of the fourth flow path is connected to the reaction device. When the first port is connected to the second port, the liquid circuit system is in the first state; when the first port is connected to the third port, the liquid circuit system is in the second state.

4. The hydraulic system according to claim 3, characterized in that, The rotary valve includes a common port and multiple connection ports. The common port is selectively connected to at least one of the connection ports. The common port is connected to the first port. The first flow path and the second flow path are respectively connected to the corresponding connection ports. When the rotary valve is in the first valve position, the common port is connected to the first flow path through at least one of the connection ports; When the rotary valve is in the second valve position, the common port is connected to the second flow path through the remaining connection ports.

5. The fluid circuit system according to claim 1, characterized in that, The rotary valve is provided with a first common port, a second common port, multiple connection ports, a first connecting groove and a second connecting groove. The first common port is connected to one end of the third flow path, and the other end of the third flow path is connected to the pump assembly. The second common port is connected to one end of the fourth flow path, and the other end of the fourth flow path is connected to the reaction device. When the rotary valve is in the first valve position, the first common port is connected to the first flow path through at least one of the connection ports and the first communication slot; When the rotary valve is in the second valve position, the second common port is connected to the second flow path through the remaining connection ports and the second communication slot.

6. The hydraulic system according to claim 5, characterized in that, The rotary valve includes a stator and a rotor disposed opposite to the stator. The stator is provided with a first common port, a second common port, the plurality of connection ports and at least a portion of the first connecting slot, and the rotor is provided with at least a portion of the second connecting slot.

7. The fluid circuit system according to claim 6, characterized in that, The first connecting slot includes a first slot and a second slot that can be connected, and the first slot and the second slot are respectively disposed on the stator and the rotor.

8. The fluid circuit system according to claim 7, characterized in that, The first slot has two ends, one end of which is connected to the first common port and the other end of which is connected to the second slot. The second slot has two ends, one end of which is connected to the first slot and the other end of which may be connected to one of the connection ports.

9. The fluid circuit system according to claim 8, characterized in that, The stator includes a first end face and a second end face facing away from each other, and the rotor includes a third end face and a fourth end face facing away from each other. The second end face and the third end face are in contact with each other. The first groove is formed on the first end face, and the second groove is formed on the third end face.

10. The fluid circuit system according to claim 6, characterized in that, The second connecting slot has two ends, one end of which is connected to the second common port, and the other end is optionally connected to one of the connection ports.

11. The fluid circuit system according to claim 10, characterized in that, The stator includes a first end face and a second end face facing away from each other, and the rotor includes a third end face and a fourth end face facing away from each other. The second end face and the third end face are in contact with each other, and the second connecting groove is formed on the third end face.

12. The fluid circuit system according to claim 1, characterized in that, When the fluid system is in the first state, the pump assembly also controls the liquid in the third flow path to flow back to the second flow path to clean the second flow path.

13. The fluid circuit system according to claim 1, characterized in that, The first driving mechanism includes: Fixture; A movable component is movably disposed on the fixed frame, and the first reagent needle is mounted on the movable component; A first drive assembly is disposed on the fixed frame, the first drive assembly being used to drive the movable part to move relative to the fixed frame.

14. The fluid circuit system according to claim 13, characterized in that, The fixing frame includes a first plate, a second plate, and a connecting column. The first plate and the second plate are spaced apart. The connecting column connects the first plate and the second plate. The movable part is movably sleeved on the connecting column. The first drive assembly is installed on the first plate and the second plate.

15. The fluid circuit system according to claim 13, characterized in that, The first drive assembly includes a first motor and a first lead screw connected to the first motor. The movable part is sleeved on the first lead screw, and the first motor drives the movable part to move through the first lead screw.

16. The fluid circuit system according to claim 13, characterized in that, The liquid circuit system includes a second reagent needle and a mounting base. The mounting base is mounted on the fixed frame. The second reagent needle is connected to the first flow path and / or the second flow path. The second reagent needle is mounted on the mounting base. When the movable part moves toward the first memory and / or the second memory, it causes the second reagent needle to extend into the first memory and / or the second memory.

17. The fluid circuit system according to claim 16, characterized in that, The mounting base includes a guide and a slider. The guide is mounted on the fixed frame, and the slider is movably disposed on the guide. The second reagent needle is mounted on the slider. When the movable part moves toward the first memory and / or the second memory, it drives the slider to move.

18. The fluid circuit system according to claim 17, characterized in that, A first elastic element is provided between the sliding member and the fixed frame. The first elastic element compresses and stores force when the movable member moves toward the first memory and / or the second memory, and releases the stored force after the force between the movable member and the sliding member is eliminated, so that the second reagent needle moves toward the outside of the first memory and / or the second memory.

19. The fluid circuit system according to claim 17, characterized in that, A second elastic element is connected between the slider and the second reagent needle. The second elastic element applies a force in a first direction to the second reagent needle to keep the second reagent needle on the slider. When the second reagent needle is subjected to a force in a second direction, the second elastic element is compressed under the action of the second reagent needle. The first direction is the direction in which the second reagent needle is directed toward the first memory and / or the second memory, and the second direction is opposite to the first direction.

20. The fluid circuit system according to claim 19, characterized in that, The sliding member is provided with a mounting hole, and the second reagent needle includes a needle body and a flange disposed on the needle body. The needle body passes through the sliding member, the flange is disposed in the mounting hole, and the second elastic member abuts against the flange.

21. The fluid circuit system according to claim 13, characterized in that, The fluid circuit system includes a first detection component disposed on the fixed frame, the first detection component being used to detect the position of the moving part.

22. The fluid circuit system according to claim 21, characterized in that, The first detection component includes a first photoelectric switch and a second photoelectric switch arranged at intervals along the movement direction of the movable component. The first photoelectric switch and the second photoelectric switch are used to detect the upper limit position and the lower limit position of the movable component, respectively.

23. The fluid circuit system according to claim 1, characterized in that, The first driving mechanism includes: Fixture; A movable component is movably disposed on the fixed frame, and the first reagent needle is mounted on the movable component; A first drive assembly is disposed on the fixed frame. The first drive assembly is used to drive the movable part to move relative to the fixed frame. During the movement of the movable part, the movable part can drive the cover assembly to close and press the liquid inlet or open the liquid inlet.

24. The fluid circuit system according to claim 23, characterized in that, The cover assembly includes a guide rail, a connecting frame, and a pressure cap. The guide rail is mounted on the fixed frame, the connecting frame is movably disposed on the guide rail, and the pressure cap is disposed on the connecting frame. During the movement of the movable component, the movable component can drive the connecting frame to make the pressure cap close and press against the liquid inlet or open the liquid inlet.

25. The fluid circuit system according to claim 24, characterized in that, The connecting frame is provided with a first limiting position and a second limiting position, which are spaced apart along the movement direction of the connecting frame. When the movable member moves in the first direction, the movable member can press against the first limiting position to close and press the pressure cap tightly against the liquid inlet. When the movable member moves in the second direction, the movable member can abut against the second limiting position to open the pressure cap and the liquid inlet. The first direction is the direction in which the first reagent needle faces the first memory and / or the second memory, and the second direction is opposite to the first direction.

26. The fluid circuit system according to claim 25, characterized in that, The connecting frame is provided with a groove that extends along the movement direction of the connecting frame, and the two groove walls along the movement direction of the connecting frame respectively form the first limiting position and the second limiting position.

27. The fluid circuit system according to claim 24, characterized in that, An elastic element is provided between the connecting frame and the pressure cap, and the two ends of the elastic element are respectively connected to the connecting frame and the pressure cap.

28. The fluid circuit system according to claim 27, characterized in that, The reagent kit is kept at room temperature.

29. The fluid circuit system according to claim 22, characterized in that, The first memory and the second memory are both located in the same kit, and the liquid collector and the kit are both mounted on the same pull-out device, which is used to be mounted inside the housing of the liquid circuit system by pulling out.

30. The fluid circuit system according to claim 1, characterized in that, The fluid circuit system further includes a bracket and a second drive mechanism connected to the bracket. The first memory and the second memory are both mounted on the bracket. The second drive mechanism is used to drive the bracket to move so as to move the first memory and the second memory.

31. The fluid circuit system according to claim 30, characterized in that, The second drive mechanism includes a second motor and a second lead screw connected to the second motor. The second lead screw is connected to the bracket, and the second motor drives the bracket to move through the second lead screw.

32. The fluid circuit system according to claim 30, characterized in that, The fluid circuit system includes a second detection component, which is used to detect the position of the bracket to determine the positions of the first memory and the second memory.

33. The fluid circuit system according to claim 32, characterized in that, The first memory and the second memory are both located in the same reagent kit. The second detection component includes a third photoelectric switch, a fourth photoelectric switch, a first light-blocking element, and a second light-blocking element. The third photoelectric switch and the fourth photoelectric switch are spaced apart above the tray along the movement direction of the tray. The first light-blocking element and the second light-blocking element can both abut against the reagent kit. The first light-blocking element and the second light-blocking element can both move in a direction perpendicular to the movement of the reagent kit when the reagent kit moves. When the reagent kit is in a first position, the first light-blocking element blocks the light path of the third photoelectric switch, and the second light-blocking element blocks the light path of the fourth photoelectric switch when the tray is in a second position.

34. The fluid circuit system according to claim 32, characterized in that, Both the first memory and the second memory are located in the same kit. The second detection component includes a fifth photoelectric switch and a third light-blocking element. The third light-blocking element is disposed on the bracket. When the kit is in the initial position, the third light-blocking element blocks the light path of the fifth photoelectric switch.

35. The fluid circuit system according to claim 32, characterized in that, The first memory and the second memory are both located in the same kit. The second detection component includes a third photoelectric switch, a fourth photoelectric switch and a first light-blocking element. The third photoelectric switch and the fourth photoelectric switch are spaced apart on the side of the tray along the movement direction of the tray. The first light-blocking element is disposed on the tray. When the kit is in a first position, the first light-blocking element blocks the light path of the third photoelectric switch. When the kit is in a second position, the first light-blocking element blocks the light path of the fourth photoelectric switch.

36. The fluid circuit system according to claim 32, characterized in that, The first memory and the second memory are both located in the same reagent kit. The second detection component also includes a fifth photoelectric switch and a third light-blocking element. The third light-blocking element can move in a direction perpendicular to the movement of the reagent kit during the process of placing the reagent kit into the tray, so as to block the light path of the fifth photoelectric switch.

37. The fluid circuit system according to claim 30, characterized in that, The bracket is provided with a limiting structure that restricts the movement of the first memory and the second memory relative to the bracket.

38. The fluid circuit system according to claim 30, characterized in that, The fluid system includes a cooling device, and the bracket houses the first memory and the second memory within the cooling device.

39. The fluid circuit system according to claim 38, characterized in that, The bottom of the bracket has a through hole.

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