一种壳聚糖铜纳米簇复合物及其制备方法和应用
By preparing chitosan-copper nanoclusters as nanozymes, bacterial activity can be rapidly determined using a colorimetric method, solving the problems of time-consuming, labor-intensive, and high-cost methods in existing technologies, and realizing rapid and economical bacterial activity detection.
CN118002124BActive Publication Date: 2026-07-17SHENYANG PHARMA UNIV
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHENYANG PHARMA UNIV
- Filing Date
- 2024-02-05
- Publication Date
- 2026-07-17
AI Technical Summary
Technical Problem
Existing bacterial detection technologies are time-consuming, labor-intensive, complex to operate, and expensive, making it difficult to quickly and economically determine bacterial activity.
Method used
Chitosan copper nanocluster complexes were prepared as nanozymes, and bacterial activity was detected by colorimetry. The color signal was generated by the reaction of chitosan copper nanocluster complexes with glucose oxidase and chromogenic substrate, enabling rapid and economical determination of bacterial activity.
Benefits of technology
It enables the visual assessment of bacterial activity within 30 minutes, avoiding complex operations and high costs. It is suitable for assessing bacterial activity in water bodies and determining water quality safety.
✦ Generated by Eureka AI based on patent content.
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Abstract
本发明涉及一种壳聚糖铜纳米簇复合物及其制备方法和应用，属于涉及生物检测技术领域。一种壳聚糖铜纳米簇复合物的制备方法将L‑半胱氨酸溶液和硫酸铜溶液混合均匀，还原Cu2+，离心取上清，得到铜纳米簇溶液；将壳聚糖与铜纳米簇溶液混合搅拌，即得含有壳聚糖铜纳米簇复合物CS@CuNCs的溶液。本发明制备了壳聚糖铜纳米簇复合物纳米酶，并利用该纳米酶来进行比色传感，相比于天然酶，纳米酶不仅价格低廉，且其稳定性也是天然酶无法比拟的，实现了成本低廉的检测方法。本发明提出的比色方法，可以在肉眼下进行细菌活性的判断，不需要技术人员和专业的仪器设备，可以适用于水体的细菌活力评估，用来判断水质的安全情况。
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