A submerged macrophyte transplanting rooting agent, a preparation method thereof and a method for improving the survival rate of submerged macrophyte transplantation

Abstract

The application discloses a cabomba transplanting rooting agent, and components of the cabomba rooting agent include biological regulators, emulsifiers, penetrants and solvents, and a mass ratio of the components of the cabomba rooting agent is biological regulators:emulsifiers:penetrants:solvents=1:3-5:0.5-1.5:50-100, the biological regulators are composed of indole butyric acid, gibberellin and 6-benzylaminopurine, and the application further provides a preparation method of the cabomba transplanting rooting agent and a method for improving cabomba transplanting survival rate.

Description

Technical Field

[0001] This invention belongs to the field of aquatic plant cultivation, specifically relating to a rooting agent for water shield transplantation, its preparation method, and a method for improving the survival rate of water shield transplantation. Background Technology

[0002] Water shield (scientific name: *Brasenia schreberi* JFGmel.) is an aquatic plant belonging to the genus *Brasenia* in the family Nymphaeaceae. It is also known as water shield, water chestnut vegetable, and lake vegetable. In China, water shield is a popular aquatic vegetable, prized for its tender and delicious young shoots and leaves. Rich in protein, polysaccharides, various vitamins, and minerals, it possesses properties such as clearing heat and phlegm, detoxifying, reducing swelling, anti-inflammation, and boosting immunity. It is widely considered a dual-purpose aquatic plant resource, valued for both its medicinal and culinary uses.

[0003] Water shield (Brasenia schreberi) thrives in shallow, silty water rich in organic matter, requiring fertile bottom sediment and clear water. However, despite its long history of cultivation, water shield is still grown using traditional manual methods. Growers must enter the water, which is not only arduous but also causes significant disturbance to the bottom sediment, leading to turbidity and hindering early photosynthesis and growth. Furthermore, if the water is deep or fluctuates greatly, newly planted water shield is prone to floating seedlings, resulting in a low survival rate and wasted seed stock.

[0004] In recent years, due to challenges such as environmental pollution, the yield of water shield in West Lake and Taihu Lake has gradually decreased. Lichuan, Hubei Province, with its beautiful mountains and clear waters, has become an ideal place for water shield cultivation, developing into an important industry supporting the local economy. To address the transplanting issues of water shield, leading water shield cultivation enterprises in Lichuan are actively trying to improve cultivation methods. Research shows that some modern horticultural techniques, such as deep substrate, deep-water cultivation, and rapid propagation by stem cuttings, have been introduced into the cultivation process. Although some patents involve early-maturing cultivation, irrigation and fertilization, and stem selection, in practice they still continue the traditional cultivation method of planting water shield in the mud at the bottom of the water. However, this traditional method has not effectively solved the labor pressure of underwater operations, as well as the resulting water turbidity and survival rate problems. Summary of the Invention

[0005] In view of the problems existing in the above and / or prior art, the present invention is proposed.

[0006] Therefore, the purpose of this invention is to overcome the shortcomings of the prior art by providing a rooting agent for water shield transplantation, its preparation method, and a method for improving the survival rate of water shield transplantation.

[0007] This invention provides a rooting agent for water shield transplantation. The rooting agent comprises a bioregulator, an emulsifier, a penetrant, and a solvent. The mass ratio of the components of the rooting agent is: bioregulator: emulsifier: penetrant: solvent = 1:3-5:0.5-1.5:50-100. The bioregulator is composed of indolebutyric acid, gibberellin, and 6-benzylaminopurine (6-BA).

[0008] Furthermore, the emulsifier is any one of the following: polyoxyethylene fatty alcohol ether series, Tween series, sodium dodecylbenzenesulfonate and polyethylene glycol octylphenyl ether series.

[0009] Furthermore, the penetrant is either lignin sulfonate or naphthalene sulfonate.

[0010] Furthermore, the mass ratio of the bioregulator components in indolebutyric acid:gibberellin and benzylaminopurine is 1:0.5-1.5:0.1-45.

[0011] Furthermore, the solvent is any one or a combination of ethanol, isopropanol, methyl ethyl ketone, ethylene glycol, and propylene glycol.

[0012] According to a second aspect of the present invention, a method for preparing a rooting agent for water shield transplantation is also provided, specifically: adding a solvent to a container, adding indolebutyric acid, gibberellin, and 6-benzylaminopurine (6-BA) in a mass ratio, then adding an emulsifier and a penetrant, and stirring at room temperature for 30 minutes to ensure that all components are fully mixed.

[0013] According to a second aspect of the present invention, a method for improving the survival rate of water shield transplanted plants is also provided, the specific steps of which are as follows:

[0014] A. Preparation for water shield transplanting: Use biodegradable and environmentally friendly materials to make water shield transplanting bags;

[0015] B. Substrate preparation: Take river mud or pond mud, organic fertilizer, sandy soil and water and mix them evenly in a certain proportion to ensure that they are thoroughly wet but not obviously seeping water, so as to obtain the substrate. The ratio of the mass of the organic fertilizer to the mass of the substrate is less than or equal to 1 / 8.

[0016] C. Treatment of water shield rhizomes and transplanting of water shield: Prepare a water shield rooting agent solution by mixing water shield rooting agent with tap water at a volume ratio of 1:80-120. After treatment with the solution, plant the water shield rhizomes into water shield transplanting bags, compact the roots with substrate, tie the top of the transplanting bag with a rope, pull the rope, and gently place the water shield transplanting bag together with the water shield rhizomes into the water.

[0017] Furthermore, the size of the water shield transplanting bag can hold 0.5 to 2.5 kg of substrate. The upper end of the water shield transplanting bag has a convenient buckle, which makes it easy to seal the top after the water shield transplanting strip is inserted. At the same time, the buckle can be used to gently place the water shield transplanting bag into the water.

[0018] Furthermore, the organic fertilizer is any one or a combination of well-rotted chicken manure, soybean cake fertilizer, and rapeseed cake fertilizer.

[0019] Beneficial effects of this invention:

[0020] 1. Growth regulators such as indoleacetic acid (IAA), gibberellin, and 6-BA can exert positive physiological effects in the propagation of water shield (Brasenia schreberi) cuttings, contributing to the formation of healthy roots. IAA promotes cambium cell division, promotes root development in cuttings, and regulates callus morphology; gibberellin promotes stem elongation and induces rooting in stems; 6-BA inhibits the decomposition of chlorophyll, nucleic acids, and proteins in plant leaves, promoting rooting in stems. The combined use of these three growth regulators to prepare a rooting agent for water shield can significantly promote the rooting process, reduce the damage to the plant caused by individual components, increase the success rate of cuttings, and improve the quality of water shield in the later stages.

[0021] 2. Using transplanting bags, the rhizomes of water shield are wrapped in the bags along with the substrate, preventing seedlings from floating. Planting workers do not need to enter the water; the bags can be lowered into the water from the shore or a small boat using the straps, sinking to the bottom. This reduces the labor intensity and costs of transplanting water shield, significantly improving the success rate of cuttings and the survival rate of transplanted seedlings. This method has achieved excellent results in large-scale water shield transplanting. Specific Implementation

[0022] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the examples in the specification.

[0023] Many specific details are set forth in the following description in order to provide a full understanding of the invention. However, the invention may also be practiced in other ways different from those described herein, and those skilled in the art can make similar extensions without departing from the spirit of the invention. Therefore, the invention is not limited to the specific embodiments disclosed below.

[0024] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in different places in this specification does not necessarily refer to the same embodiment, nor is it a single or selective embodiment that is mutually exclusive with other embodiments.

[0025] Example 1

[0026] A rooting agent for water shield transplantation was prepared by mixing plant bio-regulators, emulsifiers, penetrants, and solvents in a ratio of 1:3:1:75 by weight.

[0027] In a container, add 10 grams of plant growth regulators, including indolebutyric acid (2 g), gibberellin (3 g), and 6-benzylaminopurine (5 g), then add 30 grams of emulsifiers, including polyoxyethylene fatty alcohol ether (AEO-7) (15 g) and Tween-80 (15 g), and 10 grams of penetrant sodium lignosulfonate salt (10 g). Add 750 grams of solvent, including ethanol (700 g) and ethylene glycol (50 g). Stir at room temperature for 30 minutes to ensure thorough mixing of all components, thus obtaining a rooting agent for water shield transplanting.

[0028] Example 2

[0029] A rooting agent for water shield transplantation was prepared by mixing plant bio-regulators, emulsifiers, penetrants, and solvents in a ratio of 1:4:1.5:100 by weight.

[0030] In a container, add 10 grams of plant growth regulators, including indolebutyric acid (5 g), gibberellin (4.5 g), and 6-benzylaminopurine (0.5 g), then add 40 grams of emulsifiers, including sodium dodecylbenzenesulfonate (5 g) and polyethylene glycol octylphenyl ether (OP-13) (35 g), and 15 grams of sodium naphthalenesulfonic acid formaldehyde condensate (diffuser NNO) (15 g). Finally, add 100 grams of solvents, including isopropanol (900 g) and propylene glycol (100 g). Stir well at room temperature to ensure thorough mixing of all components, thus obtaining a rooting agent for water shield transplanting.

[0031] Example 3

[0032] A rooting agent for water shield transplantation was prepared by mixing plant bioregulators, emulsifiers, penetrants, and solvents in a ratio of 1:3.5:1:60 by weight.

[0033] In a container, add 10 grams of plant growth regulators, including indolebutyric acid (0.5 g), gibberellin (0.5 g), and 6-benzylaminopurine (9 g); add 35 grams of emulsifiers, including Tween-80 (10 g) and polyethylene glycol octylphenyl ether (OP-15) (25 g); add sodium lignosulfonate penetrant (10 g); and add methyl ethyl ketone (60 g) solvent. Stir well at room temperature to obtain a rooting agent for water shield transplanting.

[0034] Example 4

[0035] Indoor transplanting of water shield

[0036] A. Preparation for transplanting water shield: Use cotton cloth to make water shield transplant bags, which can hold 0.5kg of substrate. The top has a convenient buckle, which makes it easy to seal the top after the water shield transplant tape is inserted. At the same time, the buckle can be used to gently put the water shield transplant bag into the water.

[0037] B. Substrate preparation: Take river mud or pond mud, decomposed chicken manure fertilizer, sandy soil and water and mix them evenly in a certain proportion to ensure that they are thoroughly wet but not obviously seeping water, so as to obtain the substrate. The ratio of the mass of the decomposed chicken manure fertilizer to the mass of the substrate is equal to 1 / 8.

[0038] C. Treatment of Water Shield Rhizomes and Transplanting: The rooting agent for water shield prepared in Example 1 was diluted with tap water at a volume ratio of 1:80 to prepare a rooting agent solution. Water shield rhizomes were soaked in the solution for 25 minutes, then planted into water shield transplanting bags, ensuring the roots were compacted with substrate. The top of the transplanting bag was secured with a rope. Ensure that the young shoots and stems of the water shield are exposed above the bag opening. The rope should be pulled loosely enough to prevent substrate from falling out without affecting the growth of the tender stems. After the water shield rhizomes were treated with the solution, they were planted into the transplanting bags, the roots compacted with substrate. The top of the transplanting bag was secured with a rope. Holding the rope, the transplanting bag, along with the water shield rhizomes, was gently placed into water.

[0039] Example 5

[0040] Outdoor water shield transplantation

[0041] A. Preparation for water shield transplanting: Use burlap to make water shield transplanting bags, which can hold 2.5kg of substrate. The top has a convenient buckle, which makes it easy to seal the top after the water shield transplanting strip is inserted. At the same time, the buckle can be used to gently put the water shield transplanting bag into the water.

[0042] B. Substrate preparation: Take river mud or pond mud, soybean cake fertilizer, sandy soil and water and mix them evenly in a certain proportion to ensure that they are thoroughly wet but not obviously seeping water, so as to obtain the substrate. The ratio of the mass of soybean cake fertilizer to the mass of the substrate is equal to 1 / 4.

[0043] C. Treatment of water shield rhizomes and transplanting of water shield: The rooting agent for water shield prepared in Example 2 was diluted with tap water at a volume ratio of 1:120 to prepare a rooting agent solution. After soaking the water shield rhizomes in the solution for 20 minutes, they were planted into water shield transplanting bags, ensuring that the roots were compacted with substrate. The top of the transplanting bag was then tied with a rope. Care was taken to ensure that the young shoots and stems of the water shield were exposed above the bag opening. The tightness of the rope was adjusted to prevent the substrate from falling out, but also to avoid affecting the growth of the tender stems of the water shield.

[0044] Example 6:

[0045] Outdoor water shield transplantation

[0046] A. Preparation for water shield transplanting: Use burlap to make water shield transplanting bags, which can hold 2kg of substrate. The top has a convenient buckle, which makes it easy to seal the top after the water shield transplanting strip is inserted. At the same time, the buckle can be used to gently put the water shield transplanting bag into the water.

[0047] B. Substrate preparation: Take river mud or pond mud, rapeseed cake fertilizer, sandy soil and water and mix them evenly in a certain proportion to ensure that they are thoroughly wet but not obviously seeping water, so as to obtain the substrate. The ratio of the mass of rapeseed cake fertilizer to the mass of the substrate is equal to 1 / 4.

[0048] C. Treatment of water shield rhizomes and transplanting of water shield: The rooting agent for water shield prepared in Example 3 was diluted with tap water at a volume ratio of 1:100 to prepare a rooting agent solution. After soaking the water shield rhizomes in the solution for 30 minutes, they were planted into water shield transplanting bags, ensuring that the roots were compacted with substrate. The top of the transplanting bag was then tied with a rope. Care was taken to ensure that the young shoots and stems of the water shield were exposed above the bag opening. The tightness of the rope was adjusted to prevent the substrate from falling out, but also to avoid affecting the growth of the tender stems of the water shield.

[0049] Comparative Example 1:

[0050] Outdoor water shield transplantation

[0051] In this example, water shield rhizomes treated without rooting agents were used as a control. Sturdy, biodegradable, and environmentally friendly hemp was used to construct the water shield transplant bags, ensuring they could withstand the elements. The bags were designed for easy sealing, and a drawstring allowed for easy placement into the water. A mixture of pond mud, organic fertilizer, sandy soil, and water was prepared in the correct proportions, ensuring the substrate was thoroughly moistened but not overly wet. During outdoor transplanting, the untreated water shield rhizomes were inserted into the transplant bags, the roots were gently compacted, and the bag opening was secured with the drawstring. The transplant bags were then gently placed into the water, keeping the stems and leaves facing upwards to minimize disturbance to the bottom mud.

[0052] The appearance quality of the water shield transplanted in Examples 4-6 and Comparative Example 1 was inspected on day 45 after transplanting. The results are shown in Table 1.

[0053] Table 1. Appearance quality inspection results of transplanted water shield (Brasenia schreberi) in the example.

[0054]

[0055] The water shield transplanted in Examples 4-6 exhibited optimal growth, with outstanding average weight per rolled leaf. The survival rate of the water shield transplanted in Examples 4-6 was between 95-100%. In Example 5, the survival rate of the water shield cuttings reached 100%. However, in Comparative Example 1, the survival rate of the water shield transplanted was only 62%.

[0056] Nutritional quality of the water shield transplanted in Examples 4-6 and Comparative Example 1 was tested. Water shield 45 days after transplanting was taken and tested according to the agricultural standard NYT 701-2018 Water Shield and the national standard GB7718 Food Labeling. The results are shown in Table 2.

[0057] Table 2. Nutritional quality test results of transplanted water shield (Brasenia schreberi) in the examples.

[0058]

[0059]

[0060] The test results show that the water shield planted in Examples 4-6 has a particularly high water content (around 98%) and a fat content of around 0.1%. The soluble sugar, ash, crude protein, and soluble solids content in Examples 4-6 are significantly lower than those in Comparative Example 1.

[0061] The foregoing has provided a detailed description of a rooting agent for water shield transplantation, its preparation method, and a method for improving the survival rate of water shield transplantation. The embodiments used herein are merely to aid in understanding the methods and ideas of this invention; this invention includes, but is not limited to, the content of the embodiments. Various substitutions or modifications made to this invention without departing from its principles and spirit are all included within the scope of this invention.

[0062] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered by the claims of the present invention.

Claims

1. A rooting agent for water shield transplanting, characterized in that, The rooting agent for water shield transplanting comprises a bioregulator, an emulsifier, a penetrant, and a solvent. The mass ratio of the components in the rooting agent is: bioregulator: emulsifier: penetrant: solvent = 1:3-5:0.5-1.5:50-100. The bioregulator is composed of indolebutyric acid, gibberellin, and 6-benzylaminopurine. The bioregulator components are present in a mass ratio of indolebutyric acid: gibberellin: 6-benzylaminopurine of 1:0.5-1.5:0.1-45.

2. The rooting agent for water shield transplanting as described in claim 1, characterized in that, The emulsifier is any one of the following: polyoxyethylene fatty alcohol ether series, Tween series, sodium dodecylbenzenesulfonate and polyethylene glycol octylphenyl ether series.

3. The rooting agent for water shield transplanting as described in claim 1, characterized in that, The penetrant is either lignin sulfonate or naphthalene sulfonate.

4. The rooting agent for water shield transplanting as described in claim 1, characterized in that, The solvent is any one or a combination of ethanol, isopropanol, methyl ethyl ketone, ethylene glycol, and propylene glycol.

5. A method for preparing the rooting agent for water shield transplanting as described in claims 1-4, characterized in that, Specifically, add solvent to a container, then add indolebutyric acid, gibberellin, and 6-benzylaminopurine in the specified mass ratio, followed by emulsifier and penetrant. Stir at room temperature for 30-60 minutes to ensure thorough mixing of all components.

6. A method for improving the survival rate of water shield transplanted plants using the rooting agent for water shield transplanted plants as described in claims 1-4, characterized in that, The specific steps are as follows: A. Preparation for water shield transplanting: Use biodegradable and environmentally friendly materials to make water shield transplanting bags; B. Substrate preparation: Take river mud or pond mud, organic fertilizer, sandy soil and water and mix them evenly in a certain proportion, ensuring that they are thoroughly wet but not obviously seeping water, to obtain the substrate. The ratio of the mass of the organic fertilizer to the mass of the substrate is less than or equal to 1 / 8. C. Treatment of water shield rhizomes and transplanting of water shield: Prepare a rooting agent for water shield transplanting by mixing it with tap water at a volume ratio of 1:80-120. After treatment with the agent, plant the water shield rhizomes into water shield transplanting bags, compact the roots with substrate, and tie the top of the transplanting bag with a rope. Hold the rope and gently place the water shield transplanting bag together with the water shield rhizomes into the water.

7. The method for improving the survival rate of water shield transplanted as described in claim 6, characterized in that, The water shield transplanting bag can hold 0.5~2.5kg of substrate. The top of the water shield transplanting bag has a convenient buckle, which makes it easy to seal the top of the water shield transplanting bag after it is planted. At the same time, the buckle can be used to gently put the water shield transplanting bag into the water.

8. The method for improving the survival rate of water shield transplanted as described in claim 6, characterized in that, The organic fertilizer is any one or a combination of well-rotted chicken manure, soybean cake fertilizer, and rapeseed cake fertilizer.

Images