A multi-component quantitative detection method for a traditional Chinese medicine compound preparation
CN118033003BActive Publication Date: 2026-08-28THE FIRST AFFILIATED HOSPITAL ZHEJIANG UNIV COLLEGE OF MEDICINE +1
Patent Information
- Application Number
- CN202410366618.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Priority Date
- 2024-01-19
- Filing Date
- 2024-03-28
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2044-03-28
AI Technical Summary
Technical Problem
[0008]鉴于现有技术的上述缺点、不足，本发明提供一种中药复方制剂的多成分定量检测方法，能够实现对肺感制剂中黄酮、有机酸和木脂素成分(具体包括绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、柚皮苷、新橙皮苷、黄芩苷、汉黄芩苷和连翘苷)的同步定量检测，对其中异绿原酸具有很低的检测限，检测灵敏度高，借此对肺感制剂的质量实现有效管控，解决肺感制剂多成分定量检测技术的缺失以及现有单味药材检测方法与药味部分重合的复方的检测方法无法直接应用于肺感制剂多成分含量检测的技术问题
Benefits of technology
[0025](1)本发明建立的用于肺感制剂的双波长高效液相检测法，采用特定的梯度洗脱制度，实现了对肺感制剂中绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、柚皮苷、新橙皮苷、黄芩苷、汉黄芩苷和连翘苷等10种化学成分的高效分离，解决了肺感制剂中各待测成分之间，待测成分与杂质之间的干扰问题，尤其是结构近似的成分之间的分离，实现了有机酸、黄酮、木脂素在内的10个有效成分的同步定量检测，解决肺感制剂多成分定量检测技术的缺失以及现有单味药材检测方法与药味部分重合的复方的检测方法无法直接应用于肺感制剂多成分含量检测的技术问题，为肺感制剂产品质量的管控和评价提供可靠的分析方法和工具。
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN118033003B_ABST
Abstract
The present application relates to a kind of multi-component quantitative detection method of traditional Chinese medicine compound preparation, the traditional Chinese medicine compound preparation contains 10 chemical components, namely chlorogenic acid, cryptomeria green acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, naringin, neohesperidin, baicalin, Hanhuangqin and forsythia glycoside;The detection method uses high performance liquid chromatography;Chromatographic column uses octadecylsilane bonded silica as filler, mobile phase A is acetonitrile, mobile phase B is 0.05%-0.1% phosphoric acid aqueous solution, elution gradient is as follows:0 minute mobile phase A is 10-12%;5 minute mobile phase A is 10-12%;10 minute mobile phase A is 18-20%;20 minute mobile phase A is 23-24%;40 minute mobile phase A is 30-32%;50 minute mobile phase A is 95%;10 kinds of chemical components in traditional Chinese medicine compound preparation are separated and meet chromatographic analysis requirements by gradient elution.This application provides reliable analysis method and tool for the quality control and evaluation of lung feeling preparation product.
Need to check novelty before this filing date? Find Prior Art
Citation Information
Patent Citations
Method for content determination of multiple components in traditional Chinese medicinal preparation Shuanghuanglian for injection
CN103308615A
Content determination method for heat-clearing and detoxifying oral liquid
CN114034794A