A fungicidal composition for preventing and treating pachysandra stem base rot

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a fungicidal composition for preventing and treating stem base rot of pueraria. The fungicidal composition comprises active ingredients of triflumizole, enoxastro and carvacol. The fungicidal composition provided by the present application comprises active ingredients of triflumizole, enoxastro and carvacol. Through indoor experiments, it is found that the synergistic coefficient of the fungicidal composition after compounding for inhibiting the growth of stem base rot pathogen of pueraria, i.e. Fusarium solani, is greater than 120, and the fungicidal composition shows a synergistic effect. Compared with triflumizole, the fungicidal composition can reduce the risk of drug resistance, delay the generation and development of drug resistance of the pathogen, and improve the control effect on the pathogen.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, specifically relating to a fungicide composition for preventing and controlling kudzu root stem rot. Background Technology

[0002] Ipfentrifluconazole is a novel triazole fungicide developed by BASF in recent years. CAS: 1417782-08-1, Molecular formula: C 20 H 19 ClF3N3O2, molecular weight: 425.8, structural formula as follows:

[0003]

[0004] The mechanism of action of isofentricazole is to inhibit the biosynthesis of ergosterol in pathogens, thereby disrupting the cell membrane function and inhibiting or interfering with the development of appressorium and haustorium, as well as the formation of hyphae and spores. Current research reports that isofentricazole has good control effects against cucumber powdery mildew, wheat scab, banana leaf spot, tomato gray mold, and rice blast. Furthermore, due to its chiral nature, isofentricazole, after entering organisms or the ecological environment, exhibits enantiomers that specifically bind to biomolecules such as amino acids and proteins, resulting in selective differences.

[0005] While isofenfluconazole has the advantages mentioned above, as a fungicide, its long-term use as a single component can easily lead to the development and accumulation of resistance in pathogens, thereby reducing its effectiveness in controlling diseases. Combining different pesticide components can reduce the risk of resistance and broaden the fungicidal spectrum, improving disease control. Existing technologies have disclosed combinations of isofenfluconazole with fluopyram, kasugamycin, or fluopyram, but there are no reports of combinations of isofenfluconazole with azoxystrobin or carvacrol.

[0006] The information disclosed in this background section is intended only to enhance the understanding of the overall background of the invention and should not be construed as an admission or in any way implying that the information constitutes prior art known to those skilled in the art. Summary of the Invention

[0007] The purpose of this invention is to provide a bactericidal composition for preventing and controlling kudzu root stem rot, thereby solving the problems associated with using isofentrifluconazole as a single ingredient.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] The first objective of this invention is to provide a bactericidal composition in which the active ingredient is a compound of isofentrifluconazole and tebufenozide or carvacrol.

[0010] Preferably, the mass ratio of isofentrifluconazole to tebuconazole is 1-3:18-1.

[0011] Preferably, the mass ratio of isofentrifluconazole to carvacrol is 1-12:2-1.

[0012] A second objective of this invention is to provide the application of the aforementioned bactericidal composition in the prevention and control of kudzu root stem rot.

[0013] A third objective of this invention is to provide the application of the aforementioned bactericidal composition in the preparation of pesticides for controlling kudzu root stem rot.

[0014] Preferably, the pathogen causing the kudzu stem rot is Fusarium solani.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The bactericidal composition provided by this invention consists of a compound of isofentricoconazole and azoxystrobin or carvacrol. Indoor experiments revealed that the compounded bactericidal composition exhibits a co-toxicity coefficient greater than 120 in inhibiting the growth of *Fusarium solani* mycelium, the pathogen causing stem rot of kudzu roots, demonstrating a synergistic effect. Compared to isofentricoconazole alone, it reduces the risk of drug resistance and delays the emergence and development of drug resistance in pathogens; simultaneously, it improves the control effect against pathogens. Detailed Implementation

[0017] The technical solution of this invention patent will be clearly and completely described below. Obviously, the described embodiments are only some, not all, of the embodiments of this invention. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without inventive effort are within the scope of protection of this invention.

[0018] Example

[0019] 1. Test strain

[0020] The stem base of diseased kudzu roots was taken, and the pathogen was isolated, purified, and identified using conventional tissue isolation methods. The pathogen causing kudzu stem base rot was identified as Fusarium solani.

[0021] 2. Test reagents

[0022] 96.5% isofenfluconazole technical grade, 90% tebufenozide technical grade, 92.5% carvacrol technical grade

[0023] 3. Test methods (Refer to "Guidelines for Indoor Bioassays of Pesticides - Part 2: Plate Method for Inhibition of Pathogenic Fungal Mycelial Growth")

[0024] 3.1 The test reagent was first dissolved in dimethyl sulfoxide, then diluted with 0.1% Tween-80 aqueous solution to prepare a single-agent stock solution. Multiple ratios were set up, and five mass concentration gradients were set up for each single-agent stock solution and ratio mixture according to the equal ratio method.

[0025] 3.2 Add 9 mL of PDA medium (52-55℃) and 1 mL of drug solution to a sterile conical flask, shake well, and pour into a 9 cm diameter petri dish to prepare a plate containing the drug at the corresponding concentration; and set up a blank control without the drug, with 5 replicates for each treatment.

[0026] 3.3 After the test strain was propagated on PDA medium for 3 days, mycelial cakes (5 mm in diameter) were cut from the edge of the colony using a punch. The mycelial cakes were inoculated in the center of the drug-containing plate and the blank control plate, with the mycelial side facing up. The plates were covered and placed in an incubator at 25°C. When the blank control colony grew to 2 / 3 of the plate, the colony diameter was measured using the cross-cross method, and the inhibition rate of mycelial growth by different treatments was calculated.

[0027]

[0028] 4. Data Analysis

[0029] The logarithmic values ​​of agent concentrations and the probability values ​​of mycelial growth inhibition rates were regressed using DPS software, and the EC values ​​of each agent were calculated. 50 The cotoxicity coefficient (CTC) was calculated based on Sun Yunpei's method.

[0030] 5. Measurement Results

[0031] The synergistic effect of the agent was evaluated based on the calculated co-toxicity coefficient (CTC). CTC ≤ 80 indicates antagonistic effect, 80 < CTC < 120 indicates additive effect, and CTC ≥ 120 indicates synergistic effect. The results are shown in Table 1-2.

[0032] Table 1. Results of in vitro bioactivity assays of the combination of isofenfluconazole and tebuconazole with Fusarium solani.

[0033] Ifentrifluconazole 1.538 100.000 -- -- Enoximil 3.279 46.905 -- -- Ivermectin 1: Azoxystrobin 18 2.575 59.728 49.699 120.180 Ivermectin 1: Azoxystrobin 12 1.824 84.320 50.989 165.370 Ivermectin 1: Azoxystrobin 6 0.925 166.270 54.490 305.141 Ivermectin 1: Azoxystrobin 3 2.065 74.479 60.178 123.764 Ivermectin 1: Azoxystrobin 1 1.722 89.315 73.452 121.596 Ivermectin 3: Azoxystrobin 1 1.127 136.469 86.726 157.356

[0034] As shown in Table 1, the bactericidal composition of isofenfluconazole and tebuconazole has a co-toxicity coefficient of more than 120 in inhibiting the growth of Fusarium solani, the pathogen of kudzu stem rot, and exhibits a synergistic effect.

[0035] Table 2. Results of in vitro bioactivity assays of isofenfluconazole combined with carvacrol against Fusarium solani.

[0036]

[0037]

[0038] As shown in Table 2, the bactericidal composition of isofenfluconazole and carvacrol has a co-toxicity coefficient of more than 120 in inhibiting the growth of Fusarium solani, the pathogen of kudzu stem rot, and exhibits a synergistic effect.

[0039] In summary, the active ingredient of the bactericidal composition provided by this invention is a compound of isofentricoconazole and azoxystrobin or carvacrol. Indoor experiments revealed that the compounded bactericidal composition exhibits a co-toxicity coefficient greater than 120 in inhibiting the growth of *Fusarium solani* mycelium, the pathogen causing stem rot of kudzu roots, demonstrating a synergistic effect. Compared with isofentricoconazole alone, it can reduce the risk of drug resistance and delay the emergence and development of drug resistance in pathogens; simultaneously, it can improve the control effect against pathogens.

[0040] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A bactericidal composition, characterized in that, The active ingredient of the bactericidal composition is a compound of isofenfluconazole and azoxystrobin or carvacrol; wherein, The mass ratio of isofenfluconazole to tebuconazole is 1-3:18-1; The mass ratio of isofentrifluconazole to carvacrol is 1-12:2-1.

2. The application of the bactericidal composition according to claim 1 in the prevention and control of kudzu root stem rot, characterized in that, The pathogen causing the basal rot of kudzu stems is Fusarium solani.

3. The application of the bactericidal composition according to claim 1 in the preparation of a pesticide for controlling kudzu root stem rot, characterized in that, The pathogen causing the basal rot of kudzu stems is Fusarium solani.

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