Microscope automatic oil dropping control method and device, equipment and storage medium

CN118050886BActive Publication Date: 2026-10-27ZHENGZHOU JINYU CLINICAL TESTING CENT CO LTD
View PDF 2 Cites 0 Cited by

Patent Information

Application Number
CN202410230156.2
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-02-29
Publication Date
2026-10-27
Estimated Expiration
2044-02-29

AI Technical Summary

Technical Problem

[0004]但在实践中发现,观察一个玻片的部位细胞形态时,只需要滴加一次香柏油,就能满足使用,在观察同一个玻片的过程中可能存在多次切换高倍物镜的情况,如果每转换一次高倍物镜,就滴一滴镜油,容易导致镜油的浪费及镜油过多污染显微镜等情况的出现

Benefits of technology

[0042]本发明的有益效果在于,通过根据霍尔传感器采集到的磁场信号,判断物镜是否朝靠近载物台的方向发生转动,若发生转动,利用重力传感器识别载物台上是否有玻片以及利用液体传感器识别玻片上是否存在镜油,当且仅当载物台上有玻片且玻片上未存在镜油时,确定玻片上的滴油位置区域,并在物镜转动到位之前完成自动滴油,从而不仅能够根据物镜的转动情况判定是否需要滴油,而且可以判断玻片上是否存在镜油及定位滴油位置,在需要滴油时能够实现物镜到位前完全自动滴油,可以避免显微镜切换到物镜时再手动滴油的情况,使使用过程更加连贯顺畅,同时控制方法更加智能化、高效及准确,满足用户实际需求。

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN118050886B_ABST
    Figure CN118050886B_ABST
Patent Text Reader

Abstract

The application belongs to the technical field of intelligent control, and discloses a microscope automatic oil dropping control method, device, equipment and storage medium. Whether the objective lens rotates towards the direction of approaching the objective table is judged by using a Hall sensor. If rotation occurs, whether there is a slide on the objective table is identified by using a gravity sensor, and whether there is mirror oil on the slide is identified by using a liquid sensor. Only when there is a slide on the objective table and there is no mirror oil on the slide, the oil dropping position area on the slide is determined, and automatic oil dropping is completed before the objective lens is rotated into place. Not only can it be determined whether oil needs to be dropped according to the rotation of the objective lens, but also whether there is mirror oil on the slide and the oil dropping position can be determined. When oil needs to be dropped, the objective lens is automatically dropped completely before it is in place, which can avoid the situation that the microscope needs to be manually dropped when switching to the objective lens, making the use process more coherent and smooth. At the same time, the control method is more intelligent, efficient and accurate, and meets the actual needs of users.
Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] This invention belongs to the field of intelligent control technology, specifically relating to an automatic oil-drip control method, device, equipment, and storage medium for microscopes. Background Technology

[0002] When observing cell morphology and special structures under a microscope, a high-power objective lens (100x) is required, which necessitates the use of cedarwood oil. Traditionally, cedarwood oil is added manually, the amount is uncontrollable, and the precision of the drop placement is relatively low. This often results in spillage, uncontrollable drop volume, and inaccurate placement, wasting reagents, affecting work efficiency, contaminating equipment, and even posing a risk of injury due to improper manual addition.

[0003] Currently, some existing technologies disclose automatic oil-drip devices for microscopes, which can automatically identify the use of high-power objectives, automatically locate the oil dripping position, and automatically control the amount of oil dripped. This can greatly improve the oil dripping efficiency and reduce the adverse effects of previous operation processes.

[0004] However, in practice, it has been found that when observing cell morphology on a part of a slide, only one drop of cedar oil is needed. During the observation of the same slide, there may be multiple switching of high-power objectives. If a drop of immersion oil is added every time a high-power objective is switched, it is easy to waste immersion oil and cause excessive immersion oil to contaminate the microscope.

[0005] This shows that the existing automatic oil-drip control methods for microscopes are still not intelligent enough and cannot meet the actual needs of users. Summary of the Invention

[0006] The purpose of this invention is to provide an automatic oil-drip control method, device, equipment, and storage medium for microscopes, which can be more intelligent and meet the actual needs of users.

[0007] The first aspect of this invention discloses an automatic oil-drip control method for a microscope, applied to an automatic oil-drip device. The automatic oil-drip device includes a control device and an oil-drip mechanism, a gravity sensor, a liquid sensor, a photoelectric sensor, and a Hall sensor, all communicatively connected to the control device. The gravity sensor, the liquid sensor, and the photoelectric sensor are embedded in the microscope stage. The Hall sensor is fixed to the microscope stage. A magnet is disposed on the outer wall of the objective lens, and the Hall sensor cooperates with the magnet to sense the liquid. The control device is used to execute the automatic oil-drip control method, which includes:

[0008] Based on the magnetic field signal collected by the Hall sensor, it is determined whether the objective lens has rotated toward the stage.

[0009] If the objective lens rotates towards the stage, the presence of a slide on the microscope stage is determined based on the gravity signal collected by the gravity sensor.

[0010] If there is a glass slide on the stage, the presence of immersion oil on the slide is determined based on the liquid signal collected by the liquid sensor.

[0011] The location of the oil droplet on the slide is determined based on the photoelectric signal collected by the photoelectric sensor if and only if there is a glass slide on the stage and there is no immersion oil on the glass slide.

[0012] Based on the magnetic field signal, the oil-drip mechanism is controlled to automatically drip oil into the oil-drip position area before the objective lens is rotated into position; wherein, the objective lens being rotated into position means that the objective lens is rotated to be directly above the stage.

[0013] In some embodiments, determining the oil droplet location region on the glass slide based on the photoelectric signal collected by the photoelectric sensor includes:

[0014] The glass slide is divided into multiple glass slide regions, and the photoelectric signal of each glass slide region is acquired by the photoelectric sensor.

[0015] The transmittance of each glass slide area is calculated based on the photoelectric signal of each area.

[0016] Identify the target glass slide area with a transmittance lower than the specified transmittance, and determine the oil dripping location area based on the target glass slide area.

[0017] In some embodiments, determining the oil droplet location region based on the target slide region includes:

[0018] Determine whether there are multiple target slide regions;

[0019] If there are multiple target slide regions, and at least two target slide regions are adjacent to each other, then all adjacent target slide regions are defined as the experimental area.

[0020] Obtain a region of a specified size located at the center of the experimental area and mark it as the oil dripping location region.

[0021] In some embodiments, the method further includes:

[0022] If there is only one target slide area, mark that target slide area as the oil dripping location area.

[0023] In some embodiments, controlling the oil-drip mechanism to automatically drip oil into the oil-drip position area before the objective lens is rotated into position, based on the magnetic field signal, includes:

[0024] Calculate the real-time distance between the objective lens and the microscope stage based on the magnetic field signal;

[0025] When the real-time distance is less than the specified distance, the oil-drip mechanism is controlled to start automatic oil dripping, so that the oil-drip mechanism completes the automatic oil dripping of the oil-drip position area before the objective lens is rotated into place.

[0026] The second aspect of this invention discloses an automatic oil-drip control device for a microscope, applied to an automatic oil-drip apparatus. The automatic oil-drip apparatus includes a control device and an oil-drip mechanism, a gravity sensor, a liquid sensor, a photoelectric sensor, and a Hall sensor, all communicatively connected to the control device. The gravity sensor, the liquid sensor, and the photoelectric sensor are embedded in the microscope stage. The Hall sensor is fixed to the microscope stage. A magnet is disposed on the outer wall of the objective lens, and the Hall sensor cooperates with the magnet to sense the light. The control device includes:

[0027] The first sensing unit is used to determine whether the objective lens has rotated toward the stage based on the magnetic field signal collected by the Hall sensor.

[0028] The second sensing unit is used to determine whether there is a slide on the microscope stage based on the gravity signal collected by the gravity sensor when the objective lens rotates towards the stage.

[0029] The third sensing unit is used to determine whether there is immersion oil on the slide based on the liquid signal collected by the liquid sensor when there is a slide on the stage.

[0030] The positioning unit is used to determine the oil dripping location area on the glass slide based on the photoelectric signal collected by the photoelectric sensor when there is a glass slide on the stage and there is no immersion oil on the glass slide.

[0031] An execution unit is used to control the oil-drip mechanism to automatically drip oil into the oil-drip position area before the objective lens is rotated into position, based on the magnetic field signal; wherein, rotating the objective lens into position means rotating the objective lens to be directly above the stage.

[0032] In some embodiments, the positioning unit includes:

[0033] The acquisition subunit is used to divide the glass slide into multiple glass slide regions and acquire the photoelectric signal of each glass slide region collected by the photoelectric sensor.

[0034] The calculation subunit is used to calculate the transmittance of each glass slide region based on the photoelectric signal of each glass slide region.

[0035] The first determining subunit is used to determine the target glass slide area with a transmittance lower than the specified transmittance;

[0036] The second determining subunit is used to determine the oil dripping location area based on the target glass slide area.

[0037] In some embodiments, the execution unit includes:

[0038] The ranging subunit is used to calculate the real-time distance between the objective lens and the microscope stage based on the magnetic field signal.

[0039] The start-up subunit is used to control the oil-drip mechanism to start automatic oil dripping when the real-time distance is less than the specified distance, so that the oil-drip mechanism can complete the automatic oil dripping of the oil-drip position area before the objective lens is rotated into place.

[0040] A third aspect of the present invention discloses an electronic device, including a memory storing executable program code and a processor coupled to the memory; the processor calls the executable program code stored in the memory to execute the microscope automatic oil-dispensing control method disclosed in the first aspect.

[0041] A fourth aspect of the present invention discloses a computer-readable storage medium storing a computer program, wherein the computer program causes a computer to execute the microscope automatic oil-drip control method disclosed in the first aspect.

[0042] The beneficial effects of this invention are as follows: by using the magnetic field signal collected by the Hall sensor, it determines whether the objective lens is rotating towards the stage. If rotation occurs, a gravity sensor identifies whether there is a slide on the stage and a liquid sensor identifies whether there is immersion oil on the slide. Only when there is a slide on the stage and no immersion oil is present on the slide, the oil-drip position area on the slide is determined, and automatic oil dripping is completed before the objective lens rotates to its final position. Thus, it can not only determine whether oil dripping is needed based on the rotation of the objective lens, but also determine whether there is immersion oil on the slide and locate the oil dripping position. When oil dripping is needed, it can achieve fully automatic oil dripping before the objective lens is in position, avoiding the need for manual oil dripping when switching the microscope to the objective lens, making the use process more seamless and smooth. At the same time, the control method is more intelligent, efficient, and accurate, meeting the actual needs of users. Attached Figure Description

[0043] The accompanying drawings illustrate specific examples of the technical solutions described in this invention and, together with the detailed embodiments, form part of the specification, serving to explain the technical solutions, principles, and effects of this invention.

[0044] Unless otherwise specified or defined, the same reference numerals in different figures represent the same or similar technical features, and different reference numerals may be used to represent the same or similar technical features.

[0045] Figure 1This is a structural diagram of the automatic oil-drip device for microscopes of the present invention;

[0046] Figure 2 This is a partial structural schematic diagram of the automatic oil-drip device for microscopes of the present invention;

[0047] Figure 3 This is a magnified view of point A of the automatic oil-drip device for microscopes of the present invention;

[0048] Figure 4 This is a diagram of the inside of the oil tank of the automatic oil-drip device for microscopes of the present invention;

[0049] Figure 5 This is a schematic diagram of the oil tank cover of the automatic oil-drip device for microscopes of the present invention;

[0050] Figure 6 This is a flowchart of an automatic oil-drip control method for a microscope disclosed in an embodiment of the present invention;

[0051] Figure 7 This is a schematic diagram of the structure of an automatic oil-drip control device for a microscope disclosed in an embodiment of the present invention;

[0052] Figure 8 This is a schematic diagram of the structure of an electronic device disclosed in an embodiment of the present invention.

[0053] Explanation of reference numerals in the attached figures:

[0054] 1. Base; 2. Arm; 3. Objective lens; 4. Stage; 5. Gravity sensor; 6. Liquid sensor; 7. Magnet; 8. Hall sensor; 9. Oil tank; 10. Plunger pump; 11. Piping; 12. Oil tank cap; 13. Float switch; 14. Control module; 701. First sensing unit; 702. Second sensing unit; 703. Third sensing unit; 704. Positioning unit; 705. Execution unit; 801. Memory; 802. Processor. Detailed Implementation

[0055] Unless otherwise specified or defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. When combined with the technical solutions of the invention in a real-world scenario, all technical and scientific terms used herein may also have meanings corresponding to the purpose of achieving the technical solutions of the invention. The terms "first," "second," etc., used herein are merely for distinguishing names and do not represent a specific number or order. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0056] It should be noted that when a component is considered "fixed" to another component, it can be directly fixed to the other component or there can be an intervening component; when a component is considered "connected" to another component, it can be directly connected to the other component or there can be an intervening component; when a component is considered "mounted" on another component, it can be directly mounted on the other component or there can be an intervening component; when a component is considered "placed" on another component, it can be directly placed on the other component or there can be an intervening component.

[0057] Unless otherwise specified or defined, the terms "described" or "the" as used herein refer to the technical features or technical content mentioned or described prior to the relevant section, which may be the same as or similar to the technical features or technical content mentioned herein. Furthermore, the terms "comprising" and "having," and any variations thereof, as used herein, are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or apparatus that includes a series of steps or units is not limited to the steps or units listed, but may optionally include steps or units not listed, or may optionally include other steps or units inherent to such processes, methods, products, or apparatus.

[0058] To facilitate understanding of the present invention, specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings.

[0059] like Figures 1 to 5 As shown in the figure, this invention discloses an automatic oil-drip device, including a microscope body, a control device 14, and an oil-drip mechanism, a gravity sensor 5, a liquid sensor 6, a photoelectric sensor, and a Hall sensor 8, all communicatively connected to the control device 14. The microscope body includes a base 1, an arm 2, an objective lens 3, and a stage 4. The arm 2 is mounted on the base 1, the objective lens is mounted on the arm 2, and the stage 4 is located below the objective lens 3 and is fixedly connected to the arm 2. The gravity sensor, liquid sensor, and photoelectric sensor are embedded in the stage 4 of the microscope. The Hall sensor is fixed to the stage. A magnet 7 is provided on the outer wall of the objective lens, and the Hall sensor 8 cooperates with the magnet 7 to sense signals. The gravity sensor, liquid sensor, photoelectric sensor, and Hall sensor respectively collect corresponding sensing signals and transmit them to the control device 14. They can be connected to the control device via wiring or wireless communication. The control device 14 is disposed on the microscope body and is used to execute the automatic oil-drip control method to automatically drip immersion oil onto the microscope body.

[0060] This invention discloses an automatic oil-drip control method for microscopes, applied to the aforementioned automatic oil-drip device. This method can be implemented through computer programming. The executing entity of this method can be an electronic device such as a computer, laptop, or tablet computer, or an automatic oil-drip control device for microscopes embedded in an electronic device; this invention does not limit this to any particular type.

[0061] like Figure 6 As shown, the automatic oil dripping control method includes the following steps 610-650:

[0062] 610. Based on the magnetic field signal collected by the Hall sensor, determine whether the objective lens has rotated toward the stage.

[0063] The Hall sensor is positioned at the bottom of the stage near the objective lens for observing cell morphology. A magnet 7 is attached to the microscope objective lens 3. When the objective lens 3 approaches the stage 4, the magnet 7 triggers a signal with the Hall sensor 8 located at the bottom of the stage 4 near the objective lens 3. This signal, transmitted between the magnet 7 and the Hall sensor 8, indicates whether the objective lens is rotating towards the stage. By fixing the Hall sensor to the microscope, the magnetic field signal between the Hall sensor and the magnet on the objective lens is detected when the objective lens is rotated. A specific trend in the magnetic field signal indicates that the objective lens is rotating towards the stage. When the oil immersion objective lens approaches the stage, an automatic oil dispensing signal is triggered, reducing the need for manual oil dispensing when switching the microscope to the oil immersion objective lens, resulting in a smoother and more seamless operation.

[0064] The Hall sensor 8 works as follows: A Hall semiconductor plate exists within a magnetic field. A constant current I flows from A to B through this plate. Under the influence of the Lorentz force, the electron flow of I is deflected to one side as it passes through the Hall semiconductor, creating a potential difference in the CD direction – this is the Hall voltage. The Hall voltage varies with the magnetic field strength; the stronger the magnetic field, the higher the voltage, and vice versa. The Hall voltage is very small, typically only a few millivolts, but it can be amplified by an amplifier in an integrated circuit to output a strong signal. By mounting the magnet 7 on the outer wall of the objective lens 3, when the microscope is about to switch to objective mode, indicating the need for immersion oil, the magnet 7 can trigger the Hall sensor 8 to generate a signal, thus determining whether the objective lens has rotated towards the stage.

[0065] 620. If the objective lens rotates towards the stage, determine whether there is a slide on the microscope stage based on the gravity signal collected by the gravity sensor.

[0066] In this embodiment of the invention, when a slide is placed on the stage 4, the control device can sense the gravity signal through the gravity sensor 5 to determine whether and how much gravity is acting on the stage of the microscope, thus determining whether there is a slide on the stage. Preferably, the gravity sensor 5 is an embedded miniature gravity sensor. By using an embedded miniature gravity sensor, it is not only highly sensitive but also easy to place and does not affect the actual operation of the microscope. Specifically, in step 620, the method for determining whether there is a slide on the microscope stage is as follows: the gravity value represented by the gravity signal collected by the gravity sensor is judged. If the gravity value reaches a specified gravity, it is determined that there is a slide on the stage; if the gravity value is less than the specified gravity, it is determined that there is no slide on the stage. By detecting whether there is a slide on the stage, the situation of erroneous oil dripping when there is no slide can be prevented.

[0067] 630. If there is a glass slide on the stage, determine whether there is immersion oil on the glass slide based on the liquid signal collected by the liquid sensor.

[0068] The liquid sensor 6 is located to one side of the gravity sensor 5 and is mounted on the stage 4. It detects the presence of liquid on the slide to determine the presence of immersion oil. This detection of liquid (cedar oil) on the slide serves as one of the criteria for issuing an oil-drip signal, preventing repeated dripping. Specifically, the liquid sensor 6 is positioned at the center of the microscope stage 4 and is designed as an embedded miniature liquid sensor. Using an embedded miniature liquid sensor not only provides high accuracy in liquid level sensing but also offers rapid response, ease of placement, and does not interfere with the actual operation of the microscope.

[0069] 640. If and only if there is a glass slide on the stage and there is no immersion oil on the glass slide, determine the location of the oil droplet on the glass slide based on the photoelectric signal collected by the photoelectric sensor.

[0070] Optionally, the specific implementation of determining the oil droplet location area on the glass slide based on the photoelectric signal collected by the photoelectric sensor may include the following steps S11 to S14 (not shown):

[0071] S11. Divide the glass slide into multiple glass slide regions and acquire the photoelectric signal of each glass slide region collected by the photoelectric sensor.

[0072] S12. Calculate the transmittance of each glass slide region based on the photoelectric signal of each glass slide region.

[0073] S13. Identify the target glass slide area where the light transmittance is lower than the specified light transmittance.

[0074] The specified transmittance can be the average transmittance of the unprocessed film (unprocessed film).

[0075] S14. Determine the oil dripping location area based on the target glass slide area.

[0076] There may be one or more target slide regions, therefore step S14 may include the following steps S141 to S143 (not shown):

[0077] S141. Determine whether there are multiple target slide regions.

[0078] S142. If there are multiple target slide regions, and at least two target slide regions are adjacent to each other, then all adjacent target slide regions shall be defined as the experimental region.

[0079] S143. Obtain a specified size area located at the center of the experimental area and mark it as the oil dripping location area.

[0080] In some embodiments, if it is determined that there is only one target slide area, the target slide area can be marked as the oil dripping location area.

[0081] 650. Based on the magnetic field signal, control the oil-drip mechanism to complete the automatic oil dripping in the oil-drip area before the objective lens is rotated into position; whereby the objective lens is rotated into position means that the objective lens is rotated to be directly above the stage.

[0082] like Figures 1 to 5 As shown, a mounting groove is provided on the base 1 of the microscope body, and the mounting groove is opened at the bottom of the base 1. The oil dripping mechanism includes an oil tank 9, a plunger pump 10, a pipeline 11, an oil tank cover 12, and a float switch 13. The oil tank 9 is connected to the two side walls of the mounting groove. One end of the pipeline 11 is connected to the oil tank 9, and the other end of the pipeline 11 is located above the stage 4. The float switch 13 is located inside the oil tank 9, and the oil tank cover 12 is installed at the open end of the oil tank 9. The plunger pump 10 is installed at the top of the mounting groove. The input end of the plunger pump 10 is connected to the oil tank 9, and the output end of the plunger pump 10 is connected to one end of the pipeline 11. The other end of the pipeline 11 extends along the edge of the microscope arm 2 to the top of the stage 4, so as to accurately drip the immersion oil onto the slide during oil dripping.

[0083] By embedding the tubing 11 along the microscope arm and edge, the structure is simplified, avoiding any impact on the microscope's operation. The plunger pump 10 allows for precise control of the oil dripping volume, achieving the required conditions while preventing reagent waste and contamination, thus saving costs. The oil tank 9 stores cedar oil; since the amount used each time is small, its capacity does not need to be large. The float switch 13 senses the oil level in the tank; its placement prevents any interference with the microscope's operation.

[0084] In some embodiments, when the float switch senses that the oil level in the tank is less than a specified level, the float switch sinks, triggering an alarm mechanism. At this time, the control device can trigger the alarm mechanism upon detecting the sinking of the float switch, issuing an alarm message to remind the user to refuel. By setting the float switch, an oil shortage alarm can be implemented, reminding relevant personnel to refuel in a timely manner and to order oil in advance. This avoids the impact of running out of oil on the normal operation of the microscope.

[0085] Specifically, in step 650, the method of controlling the oil-drip mechanism to complete the automatic oil dripping of the oil-drip position area before the objective lens is rotated into position can be as follows: calculate the real-time distance between the objective lens and the microscope stage based on the magnetic field signal; when the real-time distance is less than the specified distance, control the oil-drip mechanism to start automatic oil dripping, so that the oil-drip mechanism completes the automatic oil dripping of the oil-drip position area before the objective lens is rotated into position.

[0086] The automatic oil dripping mechanism is controlled as follows: the plunger pump in the mechanism draws a specified amount of oil (e.g., 50, 60, or 100 microliters) from the oil tank and injects it onto the slide through a pipeline. Because the specified distance is set to allow for the dripping time from the start of pumping to completion, oil dripping can be initiated when the real-time distance between the objective lens and the stage is sufficiently close, leaving only the pre-reserved specified distance. This ensures that the dripping process is completed when the objective lens is rotated into position, demonstrating a high degree of automation.

[0087] In summary, in implementing this embodiment of the invention, the three sensors are connected to the same controller. The controller will issue a command to trigger the oil-drip mechanism only when three conditions are met simultaneously: there is a slide on the stage, there is no liquid (mainly cedar oil) on the slide, and the microscope is using an oil immersion objective (100x).

[0088] This invention not only determines whether oil needs to be applied based on the rotation of the objective lens, but also automatically applies a quantitative amount of oil to the microscope when oil is needed even when there is no oil on the slide. Crucially, it can identify whether there is oil on the slide, thus avoiding waste of immersion oil and excessive oil contamination of the microscope compared to applying oil every time the objective lens is changed.

[0089] This invention also discloses an automatic oil-drip control device for a microscope, which is applied to an automatic oil-drip device. The automatic oil-drip device includes a control device and an oil-drip mechanism, a gravity sensor, a liquid sensor, a photoelectric sensor, and a Hall sensor, which are respectively communicated and connected to the control device. The gravity sensor, liquid sensor, and photoelectric sensor are respectively embedded in the stage of the microscope, the Hall sensor is fixed to the stage of the microscope, and a magnet is provided on the outer wall of the objective lens. The Hall sensor cooperates with the magnet to sense.

[0090] like Figure 7As shown, the automatic oil-dispensing control device for the microscope includes a first sensing unit 701, a second sensing unit 702, a third sensing unit 703, a positioning unit 704, and an execution unit 705, wherein:

[0091] The first sensing unit 701 is used to determine whether the objective lens has rotated toward the stage based on the magnetic field signal collected by the Hall sensor.

[0092] The second sensing unit 702 is used to determine whether there is a slide on the microscope stage based on the gravity signal collected by the gravity sensor when the objective lens rotates toward the stage.

[0093] The third sensing unit 703 is used to determine whether there is immersion oil on the slide based on the liquid signal collected by the liquid sensor when there is a slide on the stage.

[0094] The positioning unit 704 is used to determine the oil dripping position area on the glass slide based on the photoelectric signal collected by the photoelectric sensor when there is a glass slide on the stage and there is no immersion oil on the glass slide.

[0095] The execution unit 705 is used to control the oil-drip mechanism to automatically drip oil into the oil-drip position area before the objective lens is rotated into position, based on the magnetic field signal; wherein, the objective lens being rotated into position means that the objective lens is rotated to be directly above the stage.

[0096] As an optional implementation, the positioning unit 704 includes the following sub-units (not shown):

[0097] The acquisition subunit is used to divide the glass slide into multiple glass slide regions and acquire the photoelectric signal of each glass slide region collected by the photoelectric sensor.

[0098] The calculation subunit is used to calculate the transmittance of each glass slide region based on the photoelectric signal of each glass slide region.

[0099] The first determining subunit is used to determine the target glass slide area with a transmittance lower than the specified transmittance;

[0100] The second determining subunit is used to determine the oil dripping location area based on the target glass slide area.

[0101] Further optionally, the second determining subunit includes the following modules not shown:

[0102] The judgment module is used to determine whether there are multiple target slide regions.

[0103] The adjacency module is used to determine all adjacent target slide regions as the experimental area when the judgment module determines that there are multiple target slide regions and at least two target slide regions are adjacent regions.

[0104] The first marking module is used to obtain a specified size area located at the center of the experimental area and mark it as the oil dripping location area.

[0105] Further optionally, the second determining subunit also includes a second marking module (not shown), used to mark the target slide area as the oil dripping location area when the determining module determines that there is only one target slide area.

[0106] As an optional implementation, the execution unit 705 includes the following sub-units (not shown):

[0107] The ranging subunit is used to calculate the real-time distance between the objective lens and the microscope stage based on the magnetic field signal;

[0108] The start-up subunit is used to control the oil-drip mechanism to start automatic oil dripping when the real-time distance is less than the specified distance, so that the oil-drip mechanism can complete the automatic oil dripping of the oil-drip position area before the objective lens is rotated into place.

[0109] like Figure 8 As shown, an embodiment of the present invention discloses an electronic device, including a memory 801 storing executable program code and a processor 802 coupled to the memory 801;

[0110] The processor 802 calls the executable program code stored in the memory 801 to execute the microscope automatic oil dripping control method described in the above embodiments.

[0111] This invention also discloses a computer-readable storage medium storing a computer program that causes a computer to execute the microscope automatic oil-drip control method described in the above embodiments.

[0112] The purpose of the above embodiments is to reproduce and derive the technical solution of the present invention by way of example, and to fully describe the technical solution, purpose and effect of the present invention. The purpose is to enable the public to have a more thorough and comprehensive understanding of the disclosure of the present invention, and not to limit the scope of protection of the present invention.

[0113] The above embodiments are not an exhaustive list based on the present invention, and there may be many other embodiments not listed. Any substitutions and improvements made without departing from the concept of the present invention are within the protection scope of the present invention.

Claims

1. A method for automatically controlling oil dripping in a microscope, characterized in that, An automatic oil-drip device is applied to a microscope. The automatic oil-drip device includes a control device and an oil-drip mechanism, a gravity sensor, a liquid sensor, a photoelectric sensor, and a Hall sensor, all communicatively connected to the control device. The gravity sensor, liquid sensor, and photoelectric sensor are embedded in the microscope stage. The Hall sensor is fixed to the microscope stage. A magnet is disposed on the outer wall of the objective lens, and the Hall sensor cooperates with the magnet to sense the liquid. The control device executes the automatic oil-drip control method, which includes: Based on the magnetic field signal collected by the Hall sensor, it is determined whether the objective lens has rotated toward the stage. If the objective lens rotates towards the stage, the presence of a slide on the microscope stage is determined based on the gravity signal collected by the gravity sensor. If there is a glass slide on the stage, the presence of immersion oil on the slide is determined based on the liquid signal collected by the liquid sensor. The location of the oil droplet on the slide is determined based on the photoelectric signal collected by the photoelectric sensor if and only if there is a glass slide on the stage and there is no immersion oil on the glass slide. Based on the magnetic field signal, the oil-drip mechanism is controlled to automatically drip oil into the oil-drip position area before the objective lens is rotated into position; wherein, the objective lens being rotated into position means that the objective lens is rotated to be directly above the stage.

2. The automatic oil-drip control method for microscopes as described in claim 1, characterized in that, The step of determining the oil droplet location area on the glass slide based on the photoelectric signal collected by the photoelectric sensor includes: The glass slide is divided into multiple glass slide regions, and the photoelectric signal of each glass slide region is acquired by the photoelectric sensor. The transmittance of each glass slide area is calculated based on the photoelectric signal of each area. Identify the target glass slide area with a transmittance lower than the specified transmittance, and determine the oil dripping location area based on the target glass slide area.

3. The automatic oil-drip control method for microscopes as described in claim 2, characterized in that, The oil dripping location area is determined based on the target slide area, including: Determine whether there are multiple target slide regions; If there are multiple target slide regions, and at least two target slide regions are adjacent to each other, then all adjacent target slide regions are defined as the experimental area. Obtain a region of a specified size located at the center of the experimental area and mark it as the oil dripping location region.

4. The automatic oil-drip control method for microscopes as described in claim 3, characterized in that, The method further includes: If there is only one target slide area, mark that target slide area as the oil dripping location area.

5. The automatic oil-drip control method for microscopes as described in any one of claims 1 to 4, characterized in that, The step of controlling the oil-drip mechanism to automatically drip oil into the oil-drip area before the objective lens rotates to the correct position, based on the magnetic field signal, includes: Calculate the real-time distance between the objective lens and the microscope stage based on the magnetic field signal; When the real-time distance is less than the specified distance, the oil-drip mechanism is controlled to start automatic oil dripping, so that the oil-drip mechanism completes the automatic oil dripping of the oil-drip position area before the objective lens is rotated into place.

6. An automatic oil-dispensing control device for a microscope, characterized in that, An automatic oil-drip device is applied to a microscope. The automatic oil-drip device includes a control device and an oil-drip mechanism, a gravity sensor, a liquid sensor, a photoelectric sensor, and a Hall sensor, all communicatively connected to the control device. The gravity sensor, liquid sensor, and photoelectric sensor are embedded in the microscope stage. The Hall sensor is fixed to the microscope stage. A magnet is disposed on the outer wall of the objective lens, and the Hall sensor cooperates with the magnet to sense the liquid. The control device includes: The first sensing unit is used to determine whether the objective lens has rotated toward the stage based on the magnetic field signal collected by the Hall sensor. The second sensing unit is used to determine whether there is a slide on the microscope stage based on the gravity signal collected by the gravity sensor when the objective lens rotates towards the stage. The third sensing unit is used to determine whether there is immersion oil on the slide based on the liquid signal collected by the liquid sensor when there is a slide on the stage. The positioning unit is used to determine the oil dripping location area on the glass slide based on the photoelectric signal collected by the photoelectric sensor when there is a glass slide on the stage and there is no immersion oil on the glass slide. An execution unit is used to control the oil-drip mechanism to automatically drip oil into the oil-drip position area before the objective lens is rotated into position, based on the magnetic field signal; wherein, rotating the objective lens into position means rotating the objective lens to be directly above the stage.

7. The automatic oil-drip control device for microscopes as described in claim 6, characterized in that, The positioning unit includes: The acquisition subunit is used to divide the glass slide into multiple glass slide regions and acquire the photoelectric signal of each glass slide region collected by the photoelectric sensor. The calculation subunit is used to calculate the transmittance of each glass slide region based on the photoelectric signal of each glass slide region. The first determining subunit is used to determine the target glass slide area with a transmittance lower than the specified transmittance; The second determining subunit is used to determine the oil dripping location area based on the target glass slide area.

8. The automatic oil-drip control device for microscopes as described in claim 6 or 7, characterized in that, The execution unit includes: The ranging subunit is used to calculate the real-time distance between the objective lens and the microscope stage based on the magnetic field signal. The start-up subunit is used to control the oil-drip mechanism to start automatic oil dripping when the real-time distance is less than the specified distance, so that the oil-drip mechanism completes automatic oil dripping in the oil-drip position area before the objective lens is rotated into place.

9. An electronic device, characterized in that, It includes a memory storing executable program code and a processor coupled to the memory; the processor calls the executable program code stored in the memory to execute the microscope automatic oil-drip control method according to any one of claims 1 to 5.

10. A computer-readable storage medium, characterized in that, The computer-readable storage medium stores a computer program, wherein the computer program causes a computer to perform the microscope automatic oil-drip control method according to any one of claims 1 to 5.

Citation Information

Patent Citations

  • Stage-free automatic microscope scanning system

    CN107479178A

  • Film reading machine and oil dripping control method thereof

    CN115903203A