Image scanning method based on a linkage scanning device

Abstract

The application adopts a slide clamp transfer device to take out the slide clamp in the slide clamp cabinet and move it under a preview lens for preview shooting, marks the area on the slide that needs to be shot, positions the slide clamp on each two-dimensional moving table, drives each zoom scanning lens to uniformly rise to the initial height and shoot each horizontal area that needs to be shot on a single height layer, drives each zoom scanning lens to uniformly descend multiple times and shoot the images of each height layer, synthesizes the pictures of multiple height layers of the same horizontal area, and splices the synthesized pictures according to the horizontal position for subsequent use, thereby solving the problems of the traditional centralized placement type scanning cabinet, the overall large cabinet body, and high cost.

Description

Technical Field

[0001] This invention relates to the field of cell image scanning, and in particular to an image scanning method based on a linkage scanning device. Background Technology

[0002] After cervical cell samples are collected, they need to be prepared into slides and stained. After staining, a coverslip is placed on top to form a slide. Subsequently, the slide is placed in a slide holder and inserted into a slide scanner to scan the pathological areas into images for subsequent use by physicians.

[0003] Scanning microscopes with low magnification capture images with a large field of view, but details are lost when magnified due to lens resolution limitations. Scanning microscopes with high magnification capture images with a small field of view and clear details, but cannot observe the entire pathological area. Therefore, image stitching is required for manual review, which is time-consuming and labor-intensive. Therefore, a scanning microscope with appropriate magnification and a suitable shooting distance are generally selected for image acquisition.

[0004] Currently, common image acquisition devices on the market employ a three-axis moving mechanism equipped with an electronic scanning mirror, capable of capturing images of different areas and depths on a slide and automatically transmitting them to a backend server. Recently, due to a significant increase in inspection demands, cabinet-style scanning matrices that centrally arrange multiple 3D scanners have emerged. However, since these essentially involve assembling multiple independent scanners, the overall cabinet is quite large, and they also suffer from problems such as a large number of moving mechanisms, high cost, and high overall energy consumption. Furthermore, most of these 3D scanners are equipped with fixed-focus scanning mirrors, requiring small step distances to capture a large number of images to obtain clear details, thus resulting in low imaging efficiency. Summary of the Invention

[0005] This invention provides an image scanning method based on a linkage scanning device, which solves the problems of traditional centralized scanning cabinets, which are bulky and costly.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is: an image scanning method based on a single-axis linkage scanning device, including a slide holder, a slide transfer device and a scanning tower assembly. A preview lens is provided between the slide holder and the scanning tower assembly. The scanning tower assembly is provided with multiple support layers. Each support layer is provided with multiple two-dimensional moving stages along the circumference. A slide holder is provided on each two-dimensional moving stage. A height-adjustable lifting sleeve is provided in the center of the scanning tower assembly. The lifting sleeve is provided with multiple outward arms along the circumference. A zoom scanning lens is provided at the end of each outward arm. The slide transfer device removes the slide clips from the slide clip cabinet and moves them below the preview lens for preview shooting, marking the areas on the slides that need to be photographed. The slide holder is transferred to each two-dimensional moving stage for positioning; The extension arm drives each zoom scanning lens to rise and fall to the initial height and captures the horizontal areas that need to be captured on a single height layer. The outreach arm drives each zoom scanning lens to descend multiple times in unison and capture images of each height layer; Images from multiple height layers within the same horizontal region are composited and then stitched together according to their horizontal positions for later use.

[0007] In a preferred embodiment, each slide holder has at least one corner equipped with two vertically arranged target assemblies. Each target assembly includes a centrally hollowed-out tilting frame with a hinged target plate in the central hollow portion. The target plates of the two target assemblies are of the same height. The tilting frame has a graduated area, and a weight is located at the center of the lower end of the target plate. The embodiment also includes a method for determining the imaging depth. Pre-adjust each zoom scanning lens to be vertical and at a uniform height; After each slide holder is transferred to its respective two-dimensional moving stage, each two-dimensional moving stage drives each slide holder to move horizontally. The extension arm adjusts its height multiple times. One zoom scanning lens photographs the target plate of the target assembly on the slide holder, while the other zoom scanning lenses photograph the scale area of ​​the target assembly on the other slide holders. Find the height difference between the lowest and highest slide clips according to the clear scale lines, and record it as the shooting depth; Using the top of the cell sample area on the highest slide clip as the first imaging height, the height areas within the imaging depth range are photographed in layers.

[0008] The preferred scheme includes a multi-level segmentation scanning method: The slide transfer device takes out the slide clips from the slide clip cabinet and moves them under the preview lens for previewing and shooting. The analysis software marks the distribution area of ​​the cell sample area on each slide of the slide clip and records these preview marked areas. The preview marked area is initially divided according to the first step distance; The slide transfer device continues to transfer the slides to each two-dimensional moving stage and determines the imaging depth; The extension arm descends to the first shooting height, and each two-dimensional moving stage drives the slide holder to move to the origin position below each zoom scanning lens, and sequentially shoots the nearest preview mark area according to the first set step distance; Photograph the other preview marked areas on the slide holder one by one according to the first set step distance; The extended arm descends to the second shooting height, and each two-dimensional moving stage drives the slide clamp to reset to the origin position below the zoom scanning mirror. According to the first set step distance, the nearest preview mark area and other preview mark areas are photographed in turn. Then the extended arm descends to other shooting heights again and repeats the area-by-area shooting. The clear portions of images from the same horizontal region are combined into a single fused image. Each fused image is analyzed, and blank areas without cell tissue and healthy cell areas are excluded. The locations of suspected pathological cell areas are marked. The outrigger returns to its initial height. The zoom scanning mirror zooms and increases the magnification. The suspected pathological cell region is segmented according to a second set step size, which is smaller than the first set step size. The extension arm descends to the first imaging height, and each two-dimensional moving stage drives the slide holder to move to the origin position below each zoom scanning mirror, and sequentially images the nearest suspected pathological cell area according to the second set step distance. The extended arm descends to the second imaging height, and each two-dimensional moving stage drives the slide holder to reset to the origin position below the zoom scanning mirror. According to the first set step distance, the nearest suspected pathological cell area and other suspected pathological cell areas are photographed in sequence. Then the extended arm descends to other imaging heights again and repeats the area-by-area imaging. The clear portions of images from the same horizontal region are combined into a single fused image. Each fused image is then analyzed again to exclude blank areas without cell tissue and healthy cell areas, and to mark precisely subdivided regions that closely resemble the characteristics of pathological cells. Again, at different height levels, a third set step distance was used to capture precisely subdivided areas. The third set step distance was smaller than the second set step distance. The individual images were uploaded and stitched together to synthesize an overall image of the lesion cell area.

[0009] In a preferred embodiment, the two-dimensional moving stage includes a first slide, a first lead screw and a first guide rail slider mechanism are provided on the support plate below the first slide, the lead screw nut is connected to the first slide, the slider of the first guide rail slider mechanism is connected to the first slide, a second drive motor is provided at one end of the first lead screw, a second lead screw and a second guide rail slider device are provided on the first slide, a second slide is also provided, the second slide is connected to the slider of the second guide rail slider device and the lead screw nut, the shaft end of the second lead screw is provided with a first drive motor, and the slide clamp is positioned on the second slide.

[0010] In a preferred embodiment, the scanning tower assembly has a central fixed shaft at its center, and multiple positioning sleeves are provided on the central fixed shaft in the height direction. The upper end of the positioning sleeve is sleeved with the lifting sleeve. A first pin and a second pin are also provided. The first pin passes through the positioning sleeve to connect with the central fixed shaft, and the second pin passes through the positioning sleeve to connect with the lifting sleeve.

[0011] In the preferred embodiment, a central sleeve is provided, which is sleeved with the outer wall of the lifting sleeve. One end of the extension arm is connected to the central sleeve. A locking key is provided on the central sleeve, and a vertical locking groove is provided on the outer wall of the lifting sleeve. The locking key slides in the vertical locking groove.

[0012] In the preferred embodiment, the outer wall of the central sleeve is provided with a rack, the outer wall of the lifting sleeve is provided with a sleeve frame, the lower end of the sleeve frame is provided with a lifting motor, and a gear is installed on the shaft end of the lifting motor, the gear meshing with the rack.

[0013] The beneficial effects of this invention are as follows: a cylindrical tower-type scanning component is used in conjunction with a linkage vertical moving mechanism to drive the overall lifting and lowering of the scanning mirror, reducing the number of linear mechanisms and saving costs; a target component is used to predetermine the height of each slide holder and determine the imaging depth; a multi-level area screening and imaging method is used to reduce the workload caused by multi-layer imaging and make up for the loss of scanning efficiency; after multiple screenings, the workload of image stitching and post-processing manual screening is greatly reduced, thereby improving the overall efficiency of cell image identification. Attached Figure Description

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0015] Figure 1 This is a schematic diagram of the layout of the plan.

[0016] Figure 2 This is a schematic diagram of the internal structure of the scanning tower assembly.

[0017] Figure 3 This is a schematic diagram of the lifting sleeve mechanism.

[0018] Figure 4 This is a top view of a two-dimensional moving platform.

[0019] Figure 5 This is a schematic diagram of the relative positions of the two-dimensional moving stage and the zoom scanning mirror.

[0020] Figure 6 This is a preview of the marked area.

[0021] Figure 7 This is a schematic diagram of the target components.

[0022] Figure 8 This is a side view of the target component.

[0023] Figure 9 This is a schematic diagram showing the height difference between the glass slide holders.

[0024] Figure 10 This is a schematic diagram of the layering of deep regions.

[0025] Figure 11 This is a schematic diagram of the initial segmentation of the preview marked area.

[0026] Figure 12 This is a schematic diagram of screening for suspected pathological cell regions.

[0027] Figure 13 This is a schematic diagram of secondary segmentation of suspected pathological cell regions.

[0028] Figure 14 This is a schematic diagram of a cell sample when the glass slide is horizontal.

[0029] Figure 15 This is a schematic diagram showing the increasing layering in the depth direction when a cell sample is tilted.

[0030] Figure 16 This is a schematic diagram of the finely segmented regions after further regional filtering.

[0031] In the diagram: 1. Slide holder; 2. Slide transfer device; 201. Slide clamp; 3. Preview lens; 4. Scanning tower assembly; 5. Two-dimensional moving stage; 501. First slide; 502. Second slide; 503. First lead screw; 504. First guide rail slider mechanism; 505. Second lead screw; 506. Second guide rail slider device; 507. First drive motor; 508. Second drive motor; 6. Slide holder; 7. Zoom scanning mirror; 8. Lifting sleeve; 801. Vertical slot; 9. Extending arm; 901. Central sleeve; 902. Locking key; 903. Rack; 904. Gear; 905. Lifting motor; 906. Frame; 10. Target assembly; 1001. Tilt frame; 1002. Target plate; 1003. Scale area; 1004. Weight; 11. Central fixed shaft; 1101. Positioning sleeve; 1102. First pin; 1103. Detailed Implementation

[0032] like Figure 1-16 In the present invention, an image scanning method based on a single-axis linkage scanning device includes a slide holder 1, a slide transfer device 2, and a scanning tower assembly 4. A preview lens 3 is provided between the slide holder 1 and the scanning tower assembly 4. The scanning tower assembly 4 is provided with multiple support layers. Each support layer is provided with multiple two-dimensional moving stages 5 along the circumference. A slide holder 6 is provided on the two-dimensional moving stage 5. A height-adjustable lifting sleeve 8 is provided in the center of the scanning tower assembly 4. The lifting sleeve 8 is provided with multiple outward arms 9 along the circumference. A zoom scanning lens 7 is provided at the end of each outward arm 9. The slide transfer device 2 takes out the slide clip 6 from the slide clip cabinet 1 and moves it below the preview lens 3 for preview shooting, marking the area on the slide that needs to be photographed; The slide holder 6 is transferred to each two-dimensional moving stage 5 for positioning; The extension arm 9 drives each zoom scanning lens 7 to rise and fall to the initial height and captures the horizontal areas that need to be captured on the single height layer; The outreach arm 9 drives each zoom scanning lens 7 to descend multiple times in unison and capture images of each height layer; Images from multiple height layers within the same horizontal region are composited and then stitched together according to their horizontal positions for later use.

[0033] The slide transfer device 2 is equipped with three orthogonally arranged linear motion mechanisms that drive the slide clamp 201 to pick up and place the slide clamp 6 between the slide clamp cabinet 1 and the scanning tower assembly 4. The linear motion mechanism adopts a servo motor, lead screw, and guide rail mechanism.

[0034] The clip cabinet 1 has multiple clip storage positions arranged in an array in width and height.

[0035] The scanning tower assembly 4 is equipped with a rotatable turntable mechanism, which can be rotated to face the transfer device when the sheet is being loaded.

[0036] Due to machining and assembly errors of the parts, there is a slight height difference between the actual glass slide placement planes of each slide holder 6. However, at the microscopic level, this slight height difference still appears relatively large. Since the zoom scanning mirror 7 cannot be raised or lowered independently, it means that the focusing height cannot be adjusted independently. This causes the cell areas on some slide holders 6 to be in focus, while the cell areas on the remaining slide holders 6 are not in focus.

[0037] In a preferred embodiment, each slide holder 6 is further provided with at least one corner with two vertically arranged target assemblies 10. Each target assembly 10 includes a centrally hollowed-out tilting frame 1001, with a hinged target plate 1002 in the central hollow portion of the tilting frame 1001. The target plates 1002 of the two target assemblies 10 are at the same height. A scale area 1003 is provided on the tilting frame 1001, and a weight 1004 is provided at the lower center of the target plate 1002. The embodiment also includes a method for determining the imaging depth. Pre-adjust each zoom scanning mirror 7 to be vertical and at a uniform height; After each slide holder 6 is transferred to each two-dimensional moving stage 5, each two-dimensional moving stage 5 drives each slide holder 6 to move horizontally, and the extension arm 9 adjusts its height multiple times. One of the zoom scanning lenses 7 photographs the target plate 1002 of the target assembly 10 on the slide holder 6, and the other zoom scanning lenses 7 photograph the scale area 1003 of the target assembly 10 on the other slide holders 6. Find the height difference between the lowest slide clip 6 and the highest slide clip 6 according to the clear scale lines, and record it as the shooting depth; Using the top of the cell sample area on the highest slide clip 6 as the first imaging height, the height area within the imaging depth range is photographed in layers.

[0038] The weight 1004 has a low center of gravity, ensuring that the target plate 1002 remains horizontal. When the lens focuses on the center of the target plate 1002, due to the tilt angle between the target plate 1002 and the tilting bracket 1001, the graduations in the scale area 1003, except for the center line, are blurred relative to the focal plane, either above or below. The height difference reading relative to the focal plane is marked next to the graduation line. Since the height of each target plate 1002 from the slide placement plane of its respective slide holder 6 is equal and the distance is known, when the focal plane of one zoom scanning mirror 7 is taken as the height of the target plate 1002, other zoom scanning mirrors 7 can quickly determine the height difference with the zoom scanning mirror 7 that serves as the reference height by taking a reading if a certain graduation line is clear.

[0039] The area between the highest and lowest slide holders 6 is designated as the depth region to be sampled. The height reference is the height of the lower end of the coverslip of the slide in the highest slide holder 6. The number of downward imaging layers is determined based on empirical layering height. This increases the number of imaging layers in exchange for hardware cost savings by not needing to independently adjust the height of the zoom scanning mirror 7.

[0040] The preferred scheme includes a multi-level segmentation scanning method: The slide transfer device 2 takes out the slide clip 6 from the slide clip cabinet 1 and moves it under the preview lens 3 for preview shooting. The analysis software marks the distribution area of ​​the cell sample area on each slide of the slide clip 6 and records these preview marked areas. The preview marked area is initially divided according to the first step distance; The slide transfer device 2 continues to transfer the slide clip 6 to each two-dimensional moving stage 5 and determines the imaging depth; The extension arm 9 descends to the first shooting height, and each two-dimensional moving stage 5 drives the slide holder 6 to move to the origin position below each zoom scanning mirror 7, and sequentially shoots the nearest preview mark area according to the first set step distance. Photograph the other preview marked areas on the slide holder 6 in sequence according to the first set step distance; The extended arm 9 descends to the second shooting height, and each two-dimensional moving stage 5 drives the slide holder 6 to reset to the origin position below the zoom scanning mirror 7. According to the first set step distance, the nearest preview mark area and other preview mark areas are photographed in turn. Then the extended arm 9 descends to other shooting heights again and repeats the area-by-area shooting. The clear portions of images from the same horizontal region are combined into a single fused image. Each fused image is analyzed, and blank areas without cell tissue and healthy cell areas are excluded. The locations of suspected pathological cell areas are marked. Extended arm 9 returns to its initial height; The zoom scanning mirror 7 zooms and increases the magnification; The suspected pathological cell region is segmented according to a second set step size, which is smaller than the first set step size. The extension arm 9 descends to the first imaging height, and each two-dimensional moving stage 5 drives the slide holder 6 to move to the origin position below each zoom scanning mirror 7, and sequentially images the nearest suspected pathological cell area according to the second set step distance. The extended arm 9 descends to the second imaging height, and each two-dimensional moving stage 5 drives the slide holder 6 to reset to the origin position below the zoom scanning mirror 7. According to the first set step distance, the nearest suspected pathological cell area and other suspected pathological cell areas are photographed in turn. Then the extended arm 9 descends to other imaging heights again and repeats the area-by-area imaging. The clear portions of images from the same horizontal region are combined into a single fused image. Each fused image is then analyzed again to exclude blank areas without cell tissue and healthy cell areas, and to mark precisely subdivided regions that closely resemble the characteristics of pathological cells. Again, at different height levels, a third set step distance was used to capture precisely subdivided areas. The third set step distance was smaller than the second set step distance. The individual images were uploaded and stitched together to synthesize an overall image of the lesion cell area.

[0041] After the initial and second screening, the area of ​​pathological cells in the planar region is relatively small. Therefore, in the final third segmentation and imaging, the number of imaging layers can be appropriately increased to avoid some out-of-focus cell areas not being recorded due to the tilt between individual slides and slide holders. In other words, the segmentation precision in the vertical direction is positively correlated with the segmentation precision in the horizontal region.

[0042] By previewing the specific location of the marked cell sample, a wide field of view is used during the initial area-by-area imaging. This reduces the amount of planar imaging, thus offsetting the increased workload caused by increasing the number of imaging layers. At the same time, the initial and second area-by-area imaging filters out images that do not require manual verification, greatly reducing the workload of subsequent fine-division imaging and also reducing the workload of manual verification by physicians in the later stages.

[0043] In a preferred embodiment, the two-dimensional moving stage 5 includes a first slide 501. A first lead screw 503 and a first guide rail slider mechanism 504 are provided on the support plate below the first slide 501. The lead screw nut of the first lead screw 503 is connected to the first slide 501, and the slider of the first guide rail slider mechanism 504 is connected to the first slide 501. A second drive motor 508 is provided at one end of the first lead screw 503. A second lead screw 505 and a second guide rail slider device 506 are provided on the first slide 501. A second slide 502 is also provided. The second slide 502 is connected to the slider of the second guide rail slider device 506 and the lead screw nut of the second lead screw 505. A first drive motor 507 is provided at the shaft end of the second lead screw 505. The slide clip 6 is positioned on the second slide 502.

[0044] In a preferred embodiment, the scanning tower assembly 4 has a central fixed shaft 11 at its center. Multiple positioning sleeves 1101 are provided on the central fixed shaft 11 in the height direction. The upper end of the positioning sleeve 1101 is sleeved with the lifting sleeve 8. A first pin 1102 and a second pin 1103 are also provided. The first pin 1102 passes through the positioning sleeve 1101 to connect with the central fixed shaft 11, and the second pin 1103 passes through the positioning sleeve 1101 to connect with the lifting sleeve 8.

[0045] A positioning hole can be reserved on the central fixed shaft 11 for installing the first pin 1102. After the positioning sleeve 1101 is fixed, the upper lifting sleeve 8 is installed and fixed by the second pin 1103. Then the upper positioning sleeve 1101 and the lifting sleeve 8 are installed.

[0046] In a preferred embodiment, a central sleeve 901 is provided, which is sleeved with the outer wall of the lifting sleeve 8. One end of the extension arm 9 is connected to the central sleeve 901. A locking key 902 is provided on the central sleeve 901. A vertical locking groove 801 is provided on the outer wall of the lifting sleeve 8. The locking key 902 slides in the vertical locking groove 801.

[0047] The locking key 902 can prevent the central sleeve 901 from rotating, but can guide the central sleeve 901 to slide up and down.

[0048] In the preferred embodiment, the outer wall of the central sleeve 901 is provided with a rack 903, the outer wall of the lifting sleeve 8 is provided with a sleeve frame 906, the lower end of the sleeve frame 906 is provided with a lifting motor 905, and a gear 904 is installed on the shaft end of the lifting motor 905, which meshes with the rack 903.

[0049] The lifting motor 905 drives the central sleeve 901 to slide up and down relative to the lifting sleeve 8, simultaneously lifting the zoom scanning mirror 7 to adjust the focal plane height.

[0050] The above embodiments are merely preferred technical solutions of the present invention and should not be considered as limitations on the present invention. The scope of protection of the present invention should be limited to the technical solutions described in the claims, including equivalent substitutions of the technical features described in the claims. That is, equivalent substitutions and improvements within this scope are also within the scope of protection of the present invention.

Claims

1. An image scanning method based on a single-axis linkage scanning device, characterized in that: It includes a slide holder (1), a slide transfer device (2), and a scanning tower assembly (4). A preview lens (3) is provided between the slide holder (1) and the scanning tower assembly (4). The scanning tower assembly (4) is provided with multiple support layers. Each support layer is provided with multiple two-dimensional moving stages (5) along the circumference. A slide holder (6) is provided on the two-dimensional moving stage (5). A height-adjustable lifting sleeve (8) is provided in the center of the scanning tower assembly (4). The lifting sleeve (8) is provided with multiple outward arms (9) along the circumference. A zoom scanning lens (7) is provided at the end of each outward arm (9). The slide transfer device (2) takes out the slide clip (6) in the slide clip cabinet (1) and moves it below the preview lens (3) for preview shooting, marking the area on the slide that needs to be photographed; The slide holder (6) is transferred to each two-dimensional moving stage (5) for positioning; The extension arm (9) drives each zoom scanning lens (7) to rise and fall to the initial height and take pictures of the horizontal areas that need to be photographed on the single height layer; The extension arm (9) drives each zoom scanning lens (7) to descend multiple times in a unified manner and take images of each height layer; Images from multiple height layers within the same horizontal region are composited and then stitched together according to their horizontal positions for later use.

2. The image scanning method based on a single-axis linkage scanning device according to claim 1, characterized in that: Each slide holder (6) also has at least one corner equipped with two vertically arranged target components (10). The target component (10) includes a centrally hollowed-out tilting frame (1001). The central hollowed-out part of the tilting frame (1001) is equipped with a hinged target plate (1002). The target plates (1002) of the two target components (10) are at the same height. The tilting frame (1001) is equipped with a scale area (1003). The target plate (1002) is equipped with a weight (1004) at the lower center. The method for determining the shooting depth is also included. Pre-adjust each zoom scanning lens (7) to be vertical and at a uniform height; After each slide holder (6) is transferred to each two-dimensional moving stage (5), each two-dimensional moving stage (5) drives each slide holder (6) to move horizontally, and the extension arm (9) adjusts the height multiple times. One of the zoom scanning lenses (7) takes pictures of the target plate (1002) of the target assembly (10) on the slide holder (6), and the other zoom scanning lenses (7) take pictures of the scale area (1003) of the target assembly (10) on the other slide holders (6). Find the height difference between the lowest slide clip (6) and the highest slide clip (6) according to the clear scale lines, and record it as the shooting depth; Using the top of the cell sample area on the highest slide clip (6) as the first imaging height, the height area within the imaging depth range is imaged in layers.

3. The image scanning method based on a single-axis linkage scanning device according to claim 2, characterized in that: Including multi-level segmentation scanning methods: The slide transfer device (2) takes out the slide clip (6) in the slide clip cabinet (1) and moves it under the preview lens (3) for preview shooting. The analysis software marks the distribution area of ​​the cell sample area on each slide of the slide clip (6) and records these preview marked areas. The preview marked area is initially divided according to the first step distance; The slide transfer device (2) continues to transfer the slide clip (6) to each two-dimensional moving stage (5) and determines the imaging depth; The extension arm (9) descends to the first shooting height, and each two-dimensional moving stage (5) drives the slide holder (6) to move to the origin position below each zoom scanning lens (7), and sequentially shoots the nearest preview mark area according to the first set step distance; According to the first set step distance, take pictures of other preview marked areas on the slide holder (6) in turn; The extension arm (9) descends to the second shooting height, and each two-dimensional moving stage (5) drives the slide holder (6) to reset to the origin position below the zoom scanning mirror (7). The nearest preview mark area and other preview mark areas are photographed sequentially according to the first set step distance. Then the extension arm (9) descends to other shooting heights again and repeats the sequential shooting. The clear portions of images from the same horizontal region are combined into a single fused image. Each fused image is analyzed, and blank areas without cell tissue and healthy cell areas are excluded. The locations of suspected pathological cell areas are marked. The outrigger (9) returns to its initial height; The zoom scanning mirror (7) zooms and increases the magnification; The suspected pathological cell region is further segmented according to the second set step distance, which is smaller than the first set step distance. The extension arm (9) descends to the first imaging height, and each two-dimensional moving stage (5) drives the slide holder (6) to move to the origin position below each zoom scanning mirror (7), and sequentially photographs the nearest suspected pathological cell area according to the second set step distance; The extension arm (9) descends to the second imaging height, and each two-dimensional moving stage (5) drives the slide holder (6) to reset to the origin position below the zoom scanning mirror (7). The nearest suspected pathological cell area and other suspected pathological cell areas are photographed in sequence according to the first set step distance. Then the extension arm (9) descends to other imaging heights again and repeats the imaging of each area. The clear portions of images from the same horizontal region are combined into a single fused image. Each fused image is then analyzed again to exclude blank areas without cell tissue and healthy cell areas, and to mark precisely subdivided regions that closely resemble the characteristics of pathological cells. Again, at different height levels, the third set step distance was used to capture precisely subdivided areas. The individual images were then uploaded and stitched together to create a composite image of the lesion cell region.

4. The image scanning method based on a single-axis linkage scanning device according to claim 1, characterized in that: The two-dimensional moving stage (5) includes a first slide (501), a first lead screw (503) and a first guide rail slider mechanism (504) are provided on the bearing plate below the first slide (501), the lead screw (503) nut is connected to the first slide (501), the slider of the first guide rail slider mechanism (504) is connected to the first slide (501), a second drive motor (508) is provided at one end of the first lead screw (503), a second lead screw (505) and a second guide rail slider device (506) are provided on the first slide (501), and a second slide (502) is also provided. The second slide (502) is connected to the slider of the second guide rail slider device (506) and the lead screw (505) nut, the shaft end of the second lead screw (505) is provided with a first drive motor (507), and the slide clip (6) is positioned on the second slide (502).

5. The image scanning method based on a single-axis linkage scanning device according to claim 1, characterized in that: The scanning tower assembly (4) has a central fixed shaft (11) in the center. Multiple positioning sleeves (1101) are provided on the central fixed shaft (11) in the height direction. The upper end of the positioning sleeve (1101) is sleeved with the lifting sleeve (8). A first pin (1102) and a second pin (1103) are also provided. The first pin (1102) passes through the positioning sleeve (1101) to connect with the central fixed shaft (11), and the second pin (1103) passes through the positioning sleeve (1101) to connect with the lifting sleeve (8). A positioning hole is reserved on the central fixed shaft (11) for installing the first pin (1102). After the positioning sleeve (1101) is fixed, the upper lifting sleeve (8) is installed and the lifting sleeve (8) is fixed by the second pin (1103). Then the upper positioning sleeve (1101) and the lifting sleeve (8) are installed.

6. The image scanning method based on a single-axis linkage scanning device according to claim 5, characterized in that: It is also provided with a central sleeve (901), which is sleeved with the outer wall of the lifting sleeve (8). One end of the extension arm (9) is connected to the central sleeve (901). The central sleeve (901) is provided with a key (902). The outer wall of the lifting sleeve (8) is provided with a vertical slot (801). The key (902) slides in the vertical slot (801). The locking key (902) prevents the central sleeve (901) from rotating, but guides the central sleeve (901) to slide up and down.

7. The image scanning method based on a single-axis linkage scanning device according to claim 6, characterized in that: The outer wall of the central sleeve (901) is provided with a rack (903), the outer wall of the lifting sleeve (8) is provided with a sleeve frame (906), the lower end of the sleeve frame (906) is provided with a lifting motor (905), the shaft end of the lifting motor (905) is equipped with a gear (904), and the gear (904) meshes with the rack (903); The lifting motor (905) drives the central sleeve (901) to slide up and down relative to the lifting sleeve (8), and simultaneously lifts the zoom scanning mirror (7) to adjust the focal plane height.

Images