Antibacterial peptide gel with antibacterial efficacy and preparation method thereof

Abstract

The present application relates to the technical field of gel preparation, and discloses an antibacterial peptide gel with antibacterial efficacy and a preparation method thereof.The antibacterial peptide gel designed and synthesized by the present application is blended with polyvinyl alcohol as a carrier and antibacterial peptides.Polyvinyl alcohol is a good component of the gel, and on this basis, hydroxyethyl cellulose is used to improve the physicochemical properties and biocompatibility thereof, and diatomite is used as a filler of the gel.At the same time, the present application modifies chitin, which is added to the antibacterial gel as an antibacterial component with better hydrophilicity, greatly enhancing the antibacterial performance of the antibacterial gel, achieving a rapid sterilization effect on the surface of the skin while ensuring accelerated healing of a wound, and meanwhile, the antibacterial gel has good stability, tensile strength and water retention, thereby enhancing the comprehensive performance of the gel.

Description

Technical Field

[0001] This invention relates to the field of gel preparation technology, specifically to an antimicrobial peptide gel with antibacterial effects and its preparation method. Background Technology

[0002] Microorganisms are widely distributed and small in size. While they have a series of beneficial effects, they also have noteworthy hazards. Microorganisms can easily lead to the spread of infectious diseases, corrosion of industrial equipment, food spoilage, and other problems, thus causing more serious issues. Common gels on the market cannot guarantee that while they have strong bactericidal ability, they also have a long bactericidal time and cannot promote the growth of wound tissue.

[0003] Antimicrobial peptides, as substances naturally extracted from bacteria, plants, etc., can quickly detect and kill targets due to their small molecular weight, good thermal stability, and broad-spectrum antimicrobial activity. At the same time, antimicrobial peptides are important defense molecules in the immune system of organisms in nature, and have good resistance to a variety of microorganisms and pathogens. Therefore, this invention uses them as the main component of antimicrobial gel and discloses a method for preparing an antimicrobial peptide gel with antimicrobial effects. Summary of the Invention

[0004] The purpose of this invention is to provide an antimicrobial peptide gel with antibacterial effects and its preparation method, so as to solve the problems mentioned in the background art.

[0005] To solve the above-mentioned technical problems, the present invention provides the following technical solution:

[0006] A method for preparing an antimicrobial peptide gel with antibacterial properties includes the following steps:

[0007] S1: Take resin, add dichloromethane solution, stir for 30-45 min, soak, stir and then dry the resin. Wash the dried product with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add dimethylformamide, aspartic acid, p-hydroxybenzonitrile, peptide coupling agent and activator. After stirring for 30-45 min, add lysine and continue to react for 20-30 min. Add glutamic acid and react for 15-20 min. Take out the synthesized product, wash and dry it. Add cleavage reagent and stir for 2-3 h. Filter and evaporate, freeze dry to obtain pure peptide powder.

[0008] S2: Take the chitosan, add it to a sodium hydroxide solution, store it at -10 to -15℃ for 24 to 30 hours, take it out, thaw it, remove the residual sodium hydroxide, and dry it.

[0009] S3: Take dried chitosan, silane coupling agent and ethanol, mix them, heat to 60-65℃, stir magnetically until uniform, react for 6-7 hours, and then purify to obtain modified chitosan.

[0010] S4: Mix polyvinyl alcohol, tremella fruiting body extract, and modified chitosan, stir, add pure peptide powder, hydroxyethyl cellulose, graphene oxide, and diatomaceous earth, mix evenly, heat to 40-50℃, stir for 3-3.5h until completely uniform, then sonicate for 20-30min, cool, freeze-thaw the solution, dry and shape to obtain antimicrobial peptide gel.

[0011] In a more optimized form, in S1, the resin is a dichlororesin, the peptide coupling agent is O-benzotriazole-tetramethylurea hexafluorophosphate, the activator is N,N-diisopropylethylamine, and the cleavage reagent is a compound product of trifluoroacetic acid, dichloromethane, and aminobutanetriol.

[0012] In an optimized manner, the proportions of the cleavage reagent in S1 are as follows: 50–52% trifluoroacetic acid, 47.5–49% dichloromethane, and 0.5–1.5% aminobutanetriol by weight.

[0013] In a more optimized form, S1 contains the following components by weight: 20-30 parts resin, 0.5-0.8 parts aspartic acid, 0.3-0.5 parts lysine, 0.1-0.3 parts glutamic acid, 5-7 parts dimethylformamide, 2-6 parts p-hydroxybenzonitrile, 1-2 parts peptide coupling agent, 1-2 parts activator, and 10-15 parts cleavage reagent.

[0014] Ideally, the mass fraction of sodium hydroxide solution in S2 is 2.44%.

[0015] In an optimized manner, in S3, the ratio of chitosan to silane coupling agent is (2-2.5:1), and the ratio of chitosan to ethanol is 1:(30-35).

[0016] In a more optimized configuration, the silane coupling agent in S3 is KH560.

[0017] In a more optimized manner, in S4, the freeze-thaw process is repeated 2 to 3 times.

[0018] In a more optimized form, S4 contains, by weight, 4-10 parts pure peptide powder, 10-16 parts hydroxyethyl cellulose, 5-7 parts modified chitosan, 2-3 parts diatomaceous earth, 2-3 parts Tremella fuciformis extract, and 10-15 parts polyvinyl alcohol.

[0019] Compared with the prior art, the beneficial effects achieved by the present invention are:

[0020] (1) The present invention designs and synthesizes antimicrobial peptides, which are used as the main antimicrobial components of antimicrobial peptide gels. While synthesizing antimicrobial peptides using solid-state synthesis, the side chains of the peptides are modified to make the prepared antimicrobial peptides have broad-spectrum antimicrobial effects. They have killing effects on bacteria, fungi, protozoa and other microorganisms, and also have inhibitory effects on microorganisms. This is mainly achieved by destroying the cell membrane structure of microorganisms and changing the membrane voltage and osmotic pressure of microorganisms to kill pathogens.

[0021] (2) The antimicrobial peptides are mixed with hydroxyethyl cellulose, Tremella fuciformis extract, polyvinyl alcohol and other bactericidal components in a certain proportion to obtain a gel. This firstly provides a moist environment for wound healing, reduces the bacterial load on the wound, slows down the inflammatory response of the wound, promotes the healing of infected wounds, effectively reduces systemic toxicity, increases the drug concentration at the wound site, and accelerates wound healing.

[0022] (3) The modification of chitosan reduced its inherent hydrophobicity. At the same time, as a good bactericidal component, the bactericidal ability of chitosan was better utilized after modification. The water absorption increased, the porosity decreased, the water vapor loss rate was reduced, and the mechanical strength of the composite gel was improved. The gel was prepared with the ability to be used on wounds for a long time to treat wounds infected by drug-resistant bacteria, and an antibacterial gel with better comprehensive performance was obtained. Detailed Implementation

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] The preparation method of antimicrobial peptide gels with antibacterial properties includes the following chemicals:

[0025] The following quantities are by weight:

[0026] Dichloropolymer resin: Model: YJ-2150, Shandong Yuanjin New Material Co., Ltd.; Aspartic acid: Item No.: fdqwrq, Jiangsu Caiwei Biotechnology Co., Ltd.; Lysine: Brand: Huayang, Shandong Zhenghong Biotechnology Co., Ltd.; Glutamic acid: Brand: Huayang, Shandong Zhenghong Biotechnology Co., Ltd.; Polyvinyl alcohol: Item No.: P105124-500g, Shanghai Aladdin Biochemical Technology Co., Ltd.; Tremella fuciformis fruiting body extract: Item No.: SN-6556, Xi'an Shennong Biotechnology Co., Ltd.; Graphene oxide: Model: MSTN-DGO, Beijing Meiston Technology Development Co., Ltd.; Diatomaceous earth: Specification: 150 mesh, Hebei Zexu Building Materials Technology Development Co., Ltd.; Chitosan: Item No.: LS-6666, Baoji Fangcheng Biotechnology Development Co., Ltd.; Hydroxyethyl cellulose: Model: QYJ86, Hebei Yida Cellulose Co., Ltd.

[0027] Example 1: S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 0.8 parts of aspartic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir and react for 45 min. Then add 0.5 parts of lysine and react for another 30 min. Then add 0.3 parts of glutamic acid and stir for 20 min. Take out the synthesized product, wash and dry it. Then add the cleavage reagent and stir for 2 h. Filter and evaporate, freeze dry to obtain pure peptide powder.

[0028] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0029] S3: Take 3 parts of dried chitosan, 1.5 parts of KH560 and 90 parts of ethanol, mix them, heat the system to 60℃, stir magnetically until uniform, react for 6 hours, and then purify to obtain modified chitosan.

[0030] S4: Mix 10 parts polyvinyl alcohol, 3 parts Tremella fuciformis extract, and 4 parts modified chitosan. After stirring evenly, add 7 parts pure peptide powder, 15 parts hydroxyethyl cellulose, 7 parts graphene oxide, and 3 parts diatomaceous earth. Mix evenly, heat to 40℃, stir for 3 hours until the reaction is complete, and then sonicate for 20 minutes. After cooling, freeze and thaw the solution twice, dry and shape to obtain antimicrobial peptide gel.

[0031] Example 2: S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 0.8 parts of aspartic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir and react for 45 min. Then add 0.5 parts of lysine and continue to react for 30 min. Take out the synthesized product, wash and dry it. Then add the cleavage reagent, stir for 2 h, filter and evaporate, and freeze dry to obtain pure peptide powder.

[0032] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0033] S3: Take 3 parts of dried chitosan, 1.5 parts of KH560 and 90 parts of ethanol, mix them, heat the system to 60℃, stir magnetically until uniform, react for 6 hours, and then purify to obtain modified chitosan.

[0034] S4: Mix 10 parts polyvinyl alcohol, 3 parts Tremella fuciformis extract, and 4 parts modified chitosan. After stirring evenly, add 7 parts pure peptide powder, 15 parts hydroxyethyl cellulose, 7 parts graphene oxide, and 3 parts diatomaceous earth. Mix evenly, heat to 40℃, stir for 3 hours until the reaction is complete, and then sonicate for 20 minutes. After cooling, freeze and thaw the solution twice, dry and shape to obtain antimicrobial peptide gel.

[0035] Example 3: S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 0.8 parts of aspartic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir, then add 0.3 parts of glutamic acid and stir for 20 min. Take out the synthesized product, wash and dry it. Add the cleavage reagent, stir for 2 h, filter and evaporate, and freeze dry to obtain pure peptide powder.

[0036] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0037] S3: Take 3 parts of dried chitosan, 1.5 parts of KH560 and 90 parts of ethanol, mix them, heat the system to 60℃, stir magnetically until uniform, react for 6 hours, and then purify to obtain modified chitosan.

[0038] S4: Mix 10 parts polyvinyl alcohol, 3 parts Tremella fuciformis extract, and 4 parts modified chitosan. After stirring evenly, add 7 parts pure peptide powder, 15 parts hydroxyethyl cellulose, 7 parts graphene oxide, and 3 parts diatomaceous earth. Mix evenly, heat to 40℃, stir for 3 hours until the reaction is complete, and then sonicate for 20 minutes. After cooling, freeze and thaw the solution twice, dry and shape to obtain antimicrobial peptide gel.

[0039] Example 4: S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 0.8 parts of aspartic acid, 0.5 parts of lysine, 0.3 parts of glutamic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir and react for 45 min. Take out the synthesized product, wash and dry it. Add the cleavage reagent and stir for 2 h. Filter and evaporate, freeze dry to obtain pure peptide powder.

[0040] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0041] S3: Take 3 parts of dried chitosan, 1.5 parts of KH560 and 90 parts of ethanol, mix them, heat the system to 60℃, stir magnetically until uniform, react for 6 hours, and then purify to obtain modified chitosan.

[0042] S4: Mix 10 parts polyvinyl alcohol, 3 parts Tremella fuciformis extract, and 4 parts modified chitosan. After stirring evenly, add 7 parts pure peptide powder, 15 parts hydroxyethyl cellulose, 7 parts graphene oxide, and 3 parts diatomaceous earth. Mix evenly, heat to 40℃, stir for 3 hours until the reaction is complete, and then sonicate for 20 minutes. After cooling, freeze and thaw the solution twice, dry and shape to obtain antimicrobial peptide gel.

[0043] Comparative Example 1: In the preparation of the antimicrobial peptide, glutamic acid and lysine were not added, but the content of aspartic acid was increased; all other conditions were the same as in Example 1.

[0044] S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 2.4 parts of aspartic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir and react for 45 min. Take out the synthesized product, wash and dry it. Add the cleavage reagent and stir for 2 h. Filter and evaporate, freeze dry to obtain pure peptide powder.

[0045] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0046] S3: Take 3 parts of dried chitosan, 1.5 parts of KH560 and 90 parts of ethanol, mix them, heat the system to 60℃, stir magnetically until uniform, react for 6 hours, and then purify to obtain modified chitosan.

[0047] S4: Mix 10 parts polyvinyl alcohol, 3 parts Tremella fuciformis extract, and 4 parts modified chitosan. After stirring evenly, add 7 parts pure peptide powder, 15 parts hydroxyethyl cellulose, 7 parts graphene oxide, and 3 parts diatomaceous earth. Mix evenly, heat to 40℃, stir for 3 hours until the reaction is complete, and then sonicate for 20 minutes. After cooling, freeze and thaw the solution twice, dry and shape to obtain antimicrobial peptide gel.

[0048] Comparative Example 2: The addition of hydroxyethyl cellulose to the antimicrobial peptide gel was omitted, and all other conditions were the same as in Example 1:

[0049] S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 0.8 parts of aspartic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir and react for 45 min. Then add 0.5 parts of lysine and react for another 30 min. Then add 0.3 parts of glutamic acid and stir for 20 min. Take out the synthesized product, wash and dry it. Then add the cleavage reagent and stir for 2 h. Filter and evaporate, freeze dry to obtain pure peptide powder.

[0050] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0051] S3: Take 3 parts of dried chitosan, 1.5 parts of KH560 and 90 parts of ethanol, mix them, heat the system to 60℃, stir magnetically until uniform, react for 6 hours, and then purify to obtain modified chitosan.

[0052] S4: Mix 10 parts polyvinyl alcohol, 3 parts tremella fruiting body extract, and 4 parts modified chitosan. After stirring evenly, add 7 parts pure peptide powder, 7 parts graphene oxide, and 3 parts diatomaceous earth. Mix evenly, heat to 40℃, stir for 3 hours until the reaction is complete, and then sonicate for 20 minutes. After cooling, freeze and thaw the solution twice, dry and shape to obtain antimicrobial peptide gel.

[0053] Comparative Example 3: Diatomaceous earth was not added during the preparation of the antimicrobial peptide gel, and all other conditions were the same as in Example 1:

[0054] S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 0.8 parts of aspartic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir and react for 45 min. Then add 0.5 parts of lysine and react for another 30 min. Then add 0.3 parts of glutamic acid and stir for 20 min. Take out the synthesized product, wash and dry it. Then add the cleavage reagent and stir for 2 h. Filter and evaporate, freeze dry to obtain pure peptide powder.

[0055] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0056] S3: Take 3 parts of dried chitosan, 1.5 parts of KH560 and 90 parts of ethanol, mix them, heat the system to 60℃, stir magnetically until uniform, react for 6 hours, and then purify to obtain modified chitosan.

[0057] S4: Mix 10 parts polyvinyl alcohol, 3 parts Tremella fuciformis extract, and 4 parts modified chitosan. After stirring evenly, add 7 parts pure peptide powder, 15 parts hydroxyethyl cellulose, and 7 parts graphene oxide. Mix evenly, heat to 40℃, stir for 3 hours until the reaction is complete, and then sonicate for 20 minutes. After cooling, freeze and thaw the solution twice, dry and shape to obtain antimicrobial peptide gel.

[0058] Comparative Example 4: The chitosan modification step was omitted, and the remaining conditions were the same as in Example 1:

[0059] S1: Take 20 parts of dichloro resin, add it to dichloromethane solution and soak. After stirring for 30 min, dry the resin. Wash the dried product three times with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add 5 parts of dimethylformamide, 0.8 parts of aspartic acid, 6 parts of p-hydroxybenzonitrile, 1.5 parts of O-benzotriazole-tetramethylurea hexafluorophosphate, and 1 part of N,N-diisopropylethylamine. Stir and react for 45 min. Then add 0.5 parts of lysine and react for another 30 min. Then add 0.3 parts of glutamic acid and stir for 20 min. Take out the synthesized product, wash and dry it. Then add the cleavage reagent and stir for 2 h. Filter and evaporate, freeze dry to obtain pure peptide powder.

[0060] S2: Take 10 parts of chitosan, add it to a 2.44% sodium hydroxide solution, stir and mix evenly, store at -10℃ for 24 hours, take it out, thaw it, remove the residual sodium hydroxide, and then dry it.

[0061] S3: Mix 10 parts polyvinyl alcohol, 3 parts Tremella fuciformis extract, and 4 parts chitosan, stir well, then add 7 parts pure peptide powder, 15 parts hydroxyethyl cellulose, 7 parts graphene oxide, and 3 parts diatomaceous earth, mix well, heat to 40℃, stir for 3 hours until the reaction is complete, then sonicate for 20 minutes, cool, freeze and thaw twice, dry and shape to obtain antimicrobial peptide gel.

[0062] Experiment: (1) Take the prepared antimicrobial peptide gel, and according to standard GB15979-2002, take the fresh culture of the 14th generation of the strain on the nutrient agar slant (18-24h), wash the bacterial moss with 5mL 0.03mol / L phosphate buffer, make the bacteria evenly suspended, and then dilute with phosphate buffer to detect its bactericidal rate after 10min.

[0063] (2) Take the prepared antimicrobial peptide gel and make it into a rectangle with a size of 10mm × 30mm. Determine the tensile strength of the gel according to the standard ASTM D638. The data obtained are shown in the table below:

[0064] Table 1 Performance Test Data

[0065] Example 1 99.5 1.91 Example 2 99.3 1.88 Example 3 98.9 1.87 Example 4 98.8 1.88 Comparative Example 1 98.1 1.73 Comparative Example 2 98.5 1.35 Comparative Example 3 98.7 1.32 Comparative Example 4 97.2 1.69

[0066] Conclusion: By comparing the data in Table 1, it can be seen that the antimicrobial peptide gel prepared according to Example 1 has better sterilization performance, can kill more bacteria in a short time, and has a wider range of applications.

[0067] The comparative examples show that the amount of pure peptides, the modification of chitosan as an additive, and hydroxyethyl cellulose are all important factors affecting the antibacterial properties of the gel during the preparation of the antibacterial gel. Hydroxyethyl cellulose and modified chitosan, as the main performance indicators of antibacterial effect, have a significant impact on the prepared antibacterial peptide gel. Graphene oxide, as an excellent loading medium for pure peptides, is also an important condition affecting the antibacterial properties.

[0068] In summary, the data shows that the method for preparing an antimicrobial peptide gel with antibacterial effects provided by the present invention can prepare an antimicrobial peptide gel with antibacterial effects.

[0069] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for preparing an antimicrobial peptide gel with antibacterial properties, characterized in that: The steps are as follows: S1: Take resin, add dichloromethane solution, stir for 30-45 min, soak, stir and then dry the resin. Wash the dried product with dimethylformamide and hexahydropyridine respectively. Under the protection of nitrogen atmosphere, add dimethylformamide, aspartic acid, p-hydroxybenzonitrile, peptide coupling agent and activator. After stirring for 30-45 min, add lysine and continue to react for 20-30 min. Add glutamic acid and react for 15-20 min. Take out the synthesized product, wash and dry it. Add cleavage reagent and stir for 2-3 h. Filter and evaporate, freeze dry to obtain pure peptide powder. S2: Take the chitosan, add it to a sodium hydroxide solution, store it at -10 to -15℃ for 24 to 30 hours, take it out, thaw it, remove the residual sodium hydroxide, and dry it. S3: Take dried chitosan, silane coupling agent and ethanol, mix them, heat to 60-65℃, stir magnetically until uniform, react for 6-7 hours, and then purify to obtain modified chitosan. S4: Mix polyvinyl alcohol, tremella fruiting body extract, and modified chitosan, stir, add pure peptide powder, hydroxyethyl cellulose, graphene oxide, and diatomaceous earth, mix evenly, heat to 40-50℃, stir for 3-3.5h until completely uniform, then sonicate for 20-30min, cool, freeze-thaw the solution, dry and shape to obtain antimicrobial peptide gel. In S1, the resin is dichlororesin, the peptide coupling agent is O-benzotriazole-tetramethylurea hexafluorophosphate, the activator is N,N-diisopropylethylamine, and the cleavage reagent is a compound product of trifluoroacetic acid, dichloromethane, and aminobutanetriol.

2. The method for preparing an antimicrobial peptide gel with antibacterial effects according to claim 1, characterized in that: In S1, the proportions of the cutting reagent are as follows: by weight, 50-52% trifluoroacetic acid, 47.5-49% dichloromethane, and 0.5-1.5% aminobutanetriol.

3. The method for preparing an antimicrobial peptide gel with antibacterial effects according to claim 1, characterized in that: S1 contains the following components by weight: 20-30 parts resin, 0.5-0.8 parts aspartic acid, 0.3-0.5 parts lysine, 0.1-0.3 parts glutamic acid, 5-7 parts dimethylformamide, 2-6 parts p-hydroxybenzonitrile, 1-2 parts peptide coupling agent, 1-2 parts activator, and 10-15 parts cleavage reagent.

4. The method for preparing an antimicrobial peptide gel with antibacterial effects according to claim 1, characterized in that: In S2, the mass fraction of sodium hydroxide solution is 2.44%.

5. The method for preparing an antimicrobial peptide gel with antibacterial effects according to claim 1, characterized in that: In S3, the mass ratio of chitosan to silane coupling agent is (2-2.5):1, and the mass ratio of chitosan to ethanol is 1:(30-35).

6. The method for preparing an antimicrobial peptide gel with antibacterial effects according to claim 1, characterized in that: In S3, the silane coupling agent is KH560.

7. The method for preparing an antimicrobial peptide gel with antibacterial effects according to claim 1, characterized in that: In S4, the freeze-thaw process is repeated 2 to 3 times.