A fungal mycelium aerogel cushion and a method of making the same

Abstract

The application discloses a kind of fungal mycelium aerogel cushion and its preparation method, belong to biotechnology material technical field.The application is prepared by using glass fiber three-dimensional felt hollow fabric 3D cloth as aggregate, and the mycelium yield of mycelium cushion prepared after drying reaches 190.2g / m 2 , mycelium thickness reaches 3.2cm, density is 59kg / m 3 , buffering coefficient reaches 4.0, rebound rate is 59.4%, the compression strength of 10% deformation reaches 317Kpa, water absorption is 3.4%.

Description

Technical Field

[0001] This invention relates to a fungal mycelium aerogel cushioning pad and its preparation method, belonging to the field of biotechnology materials technology. Technical Background

[0002] Fungal mycelium is a dense network structure formed by the branching and interconnection of tubular hyphae on a culture medium. Because the main component of mycelium, chitin, has properties such as insolubility in water, elasticity, adhesiveness, and biodegradability, fungal mycelium can be used as a natural adhesive to combine with other materials to form mycelium composite materials, which have good mechanical properties, thermal insulation properties, and flame retardant properties.

[0003] Mycelial composite materials can serve as cushioning materials. Chinese patent CN 113773665A discloses a mycelial cushioning material made from corn stalks. The corn stalks are pretreated and dried to obtain a modified solid culture medium. Fungal mycelia are then added to this modified solid culture medium for cultivation and drying to obtain the mycelial cushioning material. Chinese patent CN 106148199A discloses a method for preparing a biodegradable cushioning material using macrofungi mycelia. This method uses agricultural and forestry waste such as cottonseed hulls, bran, and sawdust as raw materials. These materials are crushed, and a certain amount of substrate nutrients are added to prepare a mycelial growth substrate, which is then placed in a sealable mold. After high-pressure wet heat sterilization, a liquid inoculum of macrofungi is inoculated into the substrate. Once the mycelia have grown to a certain extent, they are demolded for further growth, and finally dried to obtain the mycelial cushioning material. Similarly, Chinese patent CN 116692246A also discloses a low-density, high-elasticity biodegradable biomass cushioning material made from straw, sawdust, and fungal residue. However, existing materials prepared from fungal mycelium still suffer from problems such as high density, low compressive strength, high water absorption, low mycelium yield and thickness, and poor cushioning performance and resilience. Summary of the Invention

[0004] To address the above problems, this invention provides a method for preparing mycelial cushioning pads. This invention uses inorganic fabric as aggregate to composite with mycelium, resulting in mycelial cushioning pads with high density, high compressive strength, low water absorption, excellent cushioning performance and resilience, and high mycelium yield.

[0005] The first objective of this invention is to provide a method for preparing a fungal mycelium aerogel buffer material, the method comprising the following steps:

[0006] (1) Inoculate the fungus into the culture medium to activate the strain;

[0007] (2) Preparation of mushroom bags: Mix the culture medium with the nutrient solution, put it into a bag to make mushroom bags, sterilize it, cool it, inoculate the activated strain obtained in step (1) into the mushroom bags, and cultivate it.

[0008] (3) Preparation of filling fabric: Immerse the 3D hollow fabric of glass fiber three-dimensional felt in the nutrient matrix to obtain the filling fabric;

[0009] (4) Place the filling fabric obtained in step (3) onto the cultured fungal bag described in step (2) and culture it to obtain a fabric containing mycelium;

[0010] (5) Dry the fabric containing mycelium obtained in step (4), mix the dried fabric with plasticizer, plasticize it, and dry it to obtain fungal mycelium aerogel buffer material.

[0011] The culture medium contains: cottonseed hulls, corn cobs, wheat bran, and flour;

[0012] The nutrient solution contains: soluble starch, dihydrogen phosphate, peptone, glucose, calcium chloride, B complex vitamins, and mushroom extract;

[0013] The nutrient matrix contains: soluble starch, dihydrogen phosphate, peptone, glucose, calcium chloride, B complex vitamins, lentinan, wheat bran, and guar gum.

[0014] In one embodiment, the fungus includes at least one selected from Florida oyster mushroom, elm oyster mushroom, Ganoderma lucidum, king oyster mushroom, phoenix tail mushroom, enoki mushroom, and shiitake mushroom. Optionally, the fungus includes Florida oyster mushroom.

[0015] In one embodiment, the culture temperature is 25-35°C, and optionally, the culture temperature is 27°C.

[0016] In one implementation method, the culture medium in step (1) is PDA culture medium.

[0017] In one implementation method, the sterilization conditions in step (1) are high-pressure steam sterilization at 120°C and 0.1 MPa for 20 minutes.

[0018] In one embodiment, the mass ratio of cottonseed hulls, corn cobs, bran and flour in the culture medium in step (2) is (3-5):(2-3):(1-3):(1-2).

[0019] In one embodiment, the inoculation step (2) includes: soaking the wooden stick in water for more than 48 hours; mixing wheat bran and flour at a mass ratio of 1:(0.5-5); placing the wooden stick in the wheat bran and flour mixture to coat it with wheat bran and flour; sterilizing and cooling the mixture before use; inoculating the wooden stick with the strain obtained in step (1); culturing it at 25-35℃ for 5-7 days; and then mixing the wooden stick with the mushroom bag.

[0020] In one implementation, the culture time in step (2) is 5-9 days, optionally 7 days.

[0021] In one embodiment, the thickness of the 3D hollow fabric in the glass fiber three-dimensional felt in step (3) is 0.5-3cm, optionally 0.5cm, 1cm, 1.5cm, 2cm, 2.5cm or 3cm.

[0022] In one embodiment, the soaking time in step (3) is ≥12h.

[0023] In one embodiment, the nutrient solution contains: 5-20 g / L soluble starch, 0.1-1 g / L potassium dihydrogen phosphate, 0.5-2 g / L peptone, 10-30 g / L glucose, 0.5-2 g / L calcium chloride, 0.05-0.2 g / L vitamin B complex, and 0.1-1 g / L mushroom extract. Optionally, it contains: 10 g / L soluble starch, 0.5 g / L potassium dihydrogen phosphate, 1 g / L peptone, 20 g / L glucose, 1 g / L calcium chloride, 0.1 g / L vitamin B complex, and 0.5 g / L mushroom extract.

[0024] In one embodiment, the nutrient matrix contains: 10-30 g / L soluble starch, 0.1-1 g / L potassium dihydrogen phosphate, 0.5-2 g / L peptone, 10-30 g / L glucose, 0.5-2 g / L calcium chloride, 0.05-0.2 g / L vitamin B complex, 0.1-1 g / L guar gum, 50-300 g / L wheat bran, and 10-100 g / L guar gum. Optionally, it contains: 15 g / L soluble starch, 0.5 g / L potassium dihydrogen phosphate, 1 g / L peptone, 15 g / L glucose, 1 g / L calcium chloride, 0.1 g / L vitamin B complex, 0.5 g / L guar gum, 100 g / L wheat bran, and 50 g / L guar gum.

[0025] In one embodiment, the cultivation conditions in step (4) are: humidity 60-98%, CO2 concentration 1-20%, external magnetic field strength 10-100 mT, and time 5-30 days. Optionally, the cultivation temperature is 27°C, humidity 90%, CO2 concentration 3%, external magnetic field strength 50 mT, time 10 days, and the environment is protected from light.

[0026] In one embodiment, the plasticizer in step (5) includes, but is not limited to, dibutyl phthalate, dioctyl phthalate, tributyl citrate, dicyandiamide, thiocyanate, glycerol, castor oil, sorbitol, ethylene glycol, and diisooctyl sebacate. Optionally, the plasticizer is glycerol. As a preferred example, there is no limitation, and the mass concentration of the glycerol is 5%-25%, more preferably 15%.

[0027] In one embodiment, the plasticizing treatment in step (5) is to mix the plasticizer with the fabric and soak it for at least 24 hours.

[0028] The second objective of this invention is to provide a fungal mycelium aerogel buffer material prepared using the above method.

[0029] A third objective of this invention is to provide the application of the above-described method or materials in the chemical industry.

[0030] In one embodiment, the application includes the use of the above-described methods or materials in the manufacture of home sofas, car seats, and clothing and footwear.

[0031] Beneficial effects:

[0032] 1. This invention optimizes the solid culture medium formulation, the thickness of the 3D hollow fiberglass mat, and the CO2 concentration. Ultimately, under the conditions of a cottonseed hull, corn cob, bran, and flour mass ratio of 4:3:2:1, a 2cm thickness of the 3D hollow fiberglass mat, a 3% CO2 concentration, and an applied magnetic field strength of 50mT, a concentration of 190.2g / m³ was achieved. 2 The mycelium yield is high and the mycelium thickness is 3.2 cm. Higher mycelium yield can better protect the contents and increase the wear resistance of the material.

[0033] 2. This invention utilizes a biosafe plasticizer to further enhance the cushioning effect of the resulting material. When glycerol with a mass fraction of 15% is used as the plasticizer, the resulting material achieves a cushioning coefficient of 4.0 and a rebound rate of 59.4%. The decrease in the cushioning coefficient and the increase in the rebound rate indicate that the material possesses excellent cushioning performance. The cushioning material prepared by this invention exhibits good compressive strength, reaching 317 kPa at 10% deformation. The improved cushioning performance and compressive strength effectively absorb energy during drops or impacts, thus providing better protection and reducing collision damage during product transportation.

[0034] 4. The density of the buffer material prepared by this invention is significantly lower than that of existing technologies, reaching 59 kg / m³. 3 This greatly reduces material transportation costs.

[0035] 5. The water absorption rate of the buffer material prepared by this invention is significantly reduced compared with the prior art, reaching 3.4%. Therefore, it can effectively prevent the material from becoming moldy, which is beneficial to the storage of the material, expands the application scenarios of the material and extends the service life of the material, which is conducive to the promotion and application of the material. Attached Figure Description

[0036] Figure 1 Schematic diagram of the 3D fabric structure of glass fiber hollow felt.

[0037] Figure 2 : Schematic diagram of the mycelial growth environment. Detailed Implementation

[0038] To better understand the present invention, the present invention will be further described in detail below with reference to the embodiments. However, the scope of protection of the present invention is not limited to the scope represented by the embodiments.

[0039] In the following examples, the Florida oyster mushrooms were obtained from commercially available sources, the fiberglass 3D hollow fabric was obtained from Guangdong Bohao Composite Materials Co., Ltd., the compound vitamin B was purchased from Weifang Huihao Pharmaceutical Co., Ltd., and the mushroom was purchased from Shanxi Xiaxian Yaotai Biotechnology Co., Ltd.

[0040] Example 1: Preparation of mycelial buffer pads

[0041] (1) Activation of microbial strains

[0042] Florida oyster mushrooms were inoculated onto PDA medium plates and cultured at 27°C for 4 days to obtain Florida oyster mushroom strains.

[0043] (2) Solid culture of microbial strains

[0044] Take 30 wooden sticks, each about 10cm long, soak them in water for at least 48 hours, remove them, mix wheat bran and flour in a 1:1 mass ratio, coat each stick evenly, place them in a polyethylene spawn bag, seal the bag, and sterilize by high-pressure steam for 20 minutes at 120℃ and 0.1MPa. After sterilization and cooling, set aside for later use. Cut the PDA culture medium of the Florida oyster mushroom spawn obtained in step (1) into 1cm pieces on a clean bench. 3 Place the left and right squares into the sterilized mushroom bag, so that the inoculum can be inoculated onto the wooden stick. Incubate at 27℃ for 5-7 days to allow the wooden stick to be covered with mycelium.

[0045] (3) Preparation of solid culture medium and inoculation

[0046] Solid culture medium formula: by weight, 40% cottonseed hulls, 30% corn cobs, 20% wheat bran, 10% flour, with a water content of 65% (based on the dry weight of the culture medium). In addition, the water contains exogenous nutrients, the nutrient formula of which is: 10g / L soluble starch, 0.5g / L potassium dihydrogen phosphate, 1g / L peptone, 20g / L glucose, 1g / L calcium chloride, 0.1g / L compound vitamin B, and 0.5g / L mushroom extract. After the solid culture medium is mixed evenly, it is put into polypropylene bags to make mushroom bags, and then sterilized by high pressure steam at 120℃ and 0.1Mpa for 30min, and then cooled. The wooden sticks with mycelium obtained in step (2) are inserted into the mushroom bags and cultured at 27℃ for 7 days to obtain mushroom bags with well-grown mycelium.

[0047] (4) Preparation of nutrient substrate and filling fabric

[0048] To prepare the nutrient matrix, a 2cm thick 3D hollow fiberglass felt was immersed in the nutrient matrix for 12 hours, allowing the nutrient matrix to fill the 3D hollow fiberglass felt.

[0049] Nutritional matrix formula: 15g / L soluble starch, 0.5g / L potassium dihydrogen phosphate, 1g / L peptone, 15g / L glucose, 1g / L calcium chloride, 0.1g / L vitamin B complex, 0.5g / L shimata, 100g / L wheat bran powder, and 50g / L guar gum.

[0050] (5) Loosen the mycelium-covered bag, flatten it and place it in a sterilized mold. Place the fabric filled with nutrient substrate on a solid culture medium and culture it for 10 days under the conditions of 27°C, 90% humidity, 3% CO2 concentration, 50mT external magnetic field strength and no light to obtain a fabric covered with aerial mycelium.

[0051] (6) Drying treatment

[0052] The fabric covered with aerial mycelium obtained in step (5) was removed from the substrate and dried at 60°C until the moisture content was less than 14%, thus obtaining the dried mycelial aerogel buffer material.

[0053] (7) Plasticizing

[0054] Dilute glycerol with water to prepare a 15% (w / v) glycerol solution as a plasticizer. Soak the dried mycelial aerogel buffer material obtained in step (6) in the plasticizer for 24 hours to plasticize it. After plasticizing, dry it to obtain a mycelial buffer pad.

[0055] Example 2: Effect of different solid culture medium ratios on mycelial growth

[0056] The difference between Example 2 and Example 1 is that the proportion of culture medium in the solid culture medium in step (3) is different, while other conditions are the same, as shown in Table 1.

[0057] Table 1. Proportion of culture medium in different solid culture medium formulations

[0058] Group Cottonseed hulls (%) Corn cob (%) Bran (%) flour(%) 1 (Example 1) 40 30 20 10 2 30 30 30 10 3 50 30 10 10 4 30 20 30 20 5 40 20 20 20 6 50 20 10 20

[0059] The mycelial yield and thickness of mycelial buffer pads prepared with different solid culture medium formulations were tested, and the results are shown in Table 2.

[0060] Mycelium yield test: per 1m 2 Dry weight of mycelial aerogel pad.

[0061] Thickness test: The thickness of the mycelial aerogel cushioning pad after drying.

[0062] Table 2. Mycelial yield and thickness of mycelial buffer pads prepared with different solid culture medium formulations.

[0063]

[0064] As shown in Table 2, different formulations provide different carbon sources, nitrogen sources, and nutrients, resulting in different mycelial growth. In the above examples, the solid culture medium formulation used in Example 1 is more suitable for mycelial growth.

[0065] Example 3: The effect of the thickness of the 3D hollow fabric in the fiberglass three-dimensional felt on the mycelial cushioning pad

[0066] The difference between Example 3 and Example 1 is that the thickness of the 3D hollow fabric in the fiberglass three-dimensional felt in step (4) is 0.5cm, 1cm, 1.5cm, 2.5cm, and 3cm, respectively, in groups 7-11. Other conditions remain unchanged, and mycelial aerogel cushioning pads of different thicknesses are prepared according to the method described in Example 1. The mycelial yield and thickness of the prepared mycelial cushioning pads are tested. The results are shown in Table 3.

[0067] Table 3. Effects of different 3D fabric thicknesses of glass fiber 3D mat on mycelial yield and thickness.

[0068]

[0069] Table 3 shows that the mycelial yield and thickness of 3D hollow fiberglass felt vary depending on the thickness of the fabric. This is because different thicknesses provide different growth spaces for the mycelium, resulting in different mycelial contents and thus different cushioning pad thicknesses. However, a thicker fabric does not necessarily result in a thicker mycelial cushioning pad. The amount of mycelium growing on the same substrate is roughly the same. Therefore, the mycelium growing on a 3cm thick 3D hollow fiberglass felt cannot cover the fabric evenly. Thus, a thickness of 2cm yields the optimal mycelial coverage of the 3D fabric.

[0070] Example 4: Effect of CO2 concentration on mycelial aerogel buffer pads

[0071] The difference between Example 4 and Example 1 is that the CO2 concentration in step (5) is different. The CO2 concentrations for groups 12-16 are 1%, 5%, 7%, 9%, and 11%, respectively. Other conditions remain unchanged, and different mycelial aerogel buffer pads are prepared according to the steps of Example 1. The mycelial yield and thickness of the prepared mycelial aerogel buffer pads are tested, and the results are shown in Table 4.

[0072] Table 4. Effects of different CO2 concentrations on mycelial yield and thickness of mycelial aerogel cushioning pads.

[0073]

[0074] At a certain CO2 concentration, mycelia can grow more rapidly and taller, and propagate into the air to form aerial mycelia. Excessively high CO2 concentrations inhibit mycelial growth, while excessively low CO2 concentrations do not promote it. Table 4 shows that optimal mycelial growth occurs at a CO2 concentration of 3%.

[0075] Example 5: Effect of different applied magnetic field intensities on the buffering effect of the obtained mycelial aerogel buffer pad

[0076] The difference between Example 5 and Example 1 is that the applied magnetic field strength is different in step (5). The applied magnetic field strengths for groups 17-20 are 10mT, 30mT, 70mT, and 90mT, respectively. Other conditions remain unchanged, and different mycelial aerogel buffer pads are prepared according to the steps of Example 1. The mycelial yield and thickness of the prepared mycelial aerogel buffer pads are tested, and the results are shown in Table 5.

[0077] Table 5. Effects of different applied magnetic field intensities on mycelial yield and thickness of mycelial aerogel cushioning pads.

[0078] Group 1 (Example 1) 17 18 19 20 Mycelium yield (g) 190.2 130.1 149.4 183.9 174.3 Thickness (cm) 3.2 2.5 2.7 3.0 2.9

[0079] Magnetic fields can increase cell membrane permeability, facilitating the transport of nutrients between cells and inducing hyphae to grow into the air. Table 5 shows that hyphal growth is optimal when the applied magnetic field strength is 50 mT.

[0080] Example 6: Effect of different concentrations of glycerol on the buffering effect of the resulting mycelial aerogel buffer pad

[0081] The difference between Example 6 and Example 1 is that the mass fraction of glycerol in the plasticizer used in step (7) is different. The mass fraction of glycerol in groups 21-24 is 5%, 10%, 20%, and 25%, respectively. Other conditions remain unchanged. Mycelial aerogel buffer pads with different plasticizing effects are prepared according to the method of Example 1, and their buffering coefficients are tested.

[0082] The test method for the buffer coefficient refers to GB / T8168-2008 "Static Compression Test Method for Packaging Cushioning Materials". The smaller the buffer coefficient, the more impact energy absorbed per unit volume of material, and the better the buffering effect. The test results are shown in Table 6. The results show that the mycelial aerogel cushioning pad treated with 15% glycerol by mass has the lowest buffer coefficient and the best buffering effect.

[0083] Table 6. Buffer coefficients of buffer pads obtained from treatment with glycerin of different mass fractions.

[0084]

[0085] Example 7: Effect of different plasticizer treatments on the cushioning effect of the resulting mycelial aerogel cushioning pads

[0086] The difference between Example 7 and Example 1 is that the type of plasticizer in step (7) is different. The plasticizers in groups 24-26 are epoxidized soybean oil, tributyl citrate, and castor oil, respectively, and the mass fraction of the plasticizer used is 15%. With other conditions unchanged, mycelial aerogel buffer pads with different plasticizing effects can be prepared by following the steps.

[0087] The buffering coefficient of the mycelial aerogel cushioning pads obtained after plasticizing was tested. The test method for the buffering coefficient was in accordance with GB / T8168-2008 "Static Compression Test Method for Packaging Cushioning Materials". The results are shown in Table 7. The results show that the mycelial aerogel cushioning pads obtained by using glycerol as a plasticizer have the lowest buffering coefficient and the best buffering effect.

[0088] Table 7. Buffering coefficients of mycelial aerogel cushioning pads obtained from different plasticizer treatments.

[0089] Group 1 (Example 1) 24 25 26 Buffer coefficient 4.0 4.5 4.4 4.7

[0090] Comparative Example 1: Preparation of Conventional Mycelial Buffer Material

[0091] (1) Activation of microbial strains

[0092] Florida oyster mushrooms were inoculated onto PDA medium plates and cultured at 27°C for 4 days to obtain Florida oyster mushroom strains.

[0093] (2) Solid culture of microbial strains

[0094] Take 30 wooden sticks, each about 10cm long, soak them in water for at least 48 hours, remove them, mix wheat bran and flour in a 1:1 mass ratio, coat each stick evenly, place them in a polyethylene spawn bag, seal the bag, and sterilize by high-pressure steam for 20 minutes at 120℃ and 0.1MPa. After sterilization and cooling, set aside for later use. Cut the PDA culture medium of the Florida oyster mushroom spawn obtained in step (1) into 1cm pieces on a clean bench. 3 Place the left and right squares into the sterilized mushroom bag, so that the inoculum can be inoculated onto the wooden stick. Incubate at 27℃ for 5-7 days to allow the wooden stick to be covered with mycelium.

[0095] (3) Preparation of solid culture medium and inoculation

[0096] Solid culture medium formula: by weight, 40% cottonseed hulls, 30% corn cobs, 20% wheat bran, 10% flour, with a water content of 65% (based on the dry weight of the culture medium). In addition, the water contains exogenous nutrients, the nutrient formula of which is: 10g / L soluble starch, 0.5g / L potassium dihydrogen phosphate, 1g / L peptone, 20g / L glucose, 1g / L calcium chloride, 0.1g / L compound vitamin B, and 0.5g / L mushroom extract. After the solid culture medium is mixed evenly, it is put into polypropylene bags to make mushroom bags, and then sterilized by high pressure steam at 120℃ and 0.1Mpa for 30min, and then cooled. The wooden sticks with mycelium obtained in step (2) are inserted into the mushroom bags and cultured at 27℃ for 7 days to obtain mushroom bags with well-grown mycelium.

[0097] (4) Loosen the mycelium-covered bags, spread them out, and place them in a sterilized mold. Cultivate them for 10 days under conditions of 27°C, 90% humidity, 3% CO2 concentration, 50mT magnetic field strength, and no light, until the mycelium fully grows on the substrate.

[0098] (5) Drying treatment: The substrate covered with aerial mycelium is dried at 60°C until the moisture content is less than 14% to obtain mycelium composite buffer material.

[0099] Comparative Example 2: Preparation of mycelial aerogel buffer material in solid culture medium without nutrient solution

[0100] The addition of exogenous nutrients in step (3) of Example 1 is omitted, and the rest is the same as in Example 1.

[0101] Comparative Example 3: Preparation of Mycelial Aerogel Cushioning Material from Fiberglass Hollow Fabric 3D Fabric without Nutrient Matrix

[0102] The step of soaking the glass fiber 3D hollow fabric in a specific nutrient matrix in step (4) of Example 1 is omitted, and the rest is consistent with Example 1.

[0103] Comparative Example 4: Preparation of Unplasticized Mycelial Aerogel Buffer Material

[0104] Step (7) in Example 1 is omitted, and the rest remains the same as in Example 1.

[0105] Experiment Example 8: Performance Testing

[0106] The performance of the mycelial cushioning materials prepared in Example 1 and Comparative Examples 1-4 was tested, including density, cushioning coefficient, resilience, compressive strength, and water absorption rate. Density was determined according to GB8168-87 "Method for Determination of Density of Cushioning Materials". Cushioning coefficient and compressive strength were determined according to GB / T8168-2008 "Static Compression Test Method for Cushioning Materials for Packaging". Resilience was measured using a universal testing machine. Test procedure: The load was gradually increased along the thickness direction of the sample at a speed of 12±3 mm / min. When the strain reached 50%, the test was stopped, and the pressure was maintained for 3 minutes before unloading. The sample thickness was measured after 10 seconds, and the resilience rate was calculated. Water absorption rate test procedure: The initial mass of the mycelial material was weighed using an electronic balance. The mycelial material was soaked in distilled water for 12 hours. The sample was removed, and residual water was wiped off the surface. The mass after water absorption was measured. Water absorption rate = (weight after water absorption - initial weight) ÷ initial weight × 100%.

[0107] The test results are shown in Table 8.

[0108] Table 8 Performance Tests of Different Materials

[0109]

[0110] The buffer coefficient is a crucial indicator for evaluating the suitability of a buffer material for cushioning applications. A smaller buffer coefficient means more impact energy is absorbed per unit volume, resulting in better cushioning. Resilience, representing an object's ability to rebound after being compressed by external forces, is also an important indicator; a higher resilience indicates better cushioning. For buffer materials, density determines their lightness; for the same volume, lower density results in a lighter material. Compressive strength relates to a material's ability to withstand external compression; higher compressive strength means better resistance to external forces. Water absorption affects material stability; mycelial materials are prone to mold growth after absorbing water. Lower water absorption improves mold resistance and stability.

[0111] As shown in Table 8, the mycelial aerogel cushioning pad prepared in Example 1 has better cushioning performance, compression performance, water absorption rate and resilience than Comparative Examples 1, 2, 3 and 4, and is comparable to EPS foam cushioning material.

[0112] Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Anyone skilled in the art can make various modifications and alterations without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims.

Claims

1. A method for preparing a fungal mycelium aerogel buffer material, characterized in that, The method includes the following steps: (1) Inoculate the fungi into the culture medium to activate the strain; (2) Preparation of mushroom bags: Mix the culture medium with the nutrient solution, put it into bags to make mushroom bags, sterilize and cool, inoculate the activated strain obtained in step (1) into the mushroom bags and culture; (3) Preparation of filling fabric: The glass fiber three-dimensional felt hollow fabric 3D cloth is immersed in the nutrient matrix to obtain the filling fabric; the thickness of the glass fiber three-dimensional felt hollow fabric 3D cloth is 2 cm; (4) Place the filling fabric obtained in step (3) onto the cultured fungal bag described in step (2) and culture it to obtain a fabric containing mycelium; an external magnetic field was applied during the culture, the strength of which was 50 mT; the CO2 concentration during the culture was 3%; (5) Dry the fabric containing mycelium obtained in step (4), mix the dried fabric with a plasticizer, plasticize it, and dry it to obtain a fungal mycelium aerogel buffer material; the plasticizer is glycerol, and the mass-volume concentration of the glycerol is 15%; The culture medium contains cottonseed hulls, corn cobs, wheat bran, and flour in a weight ratio of 4:3:2:

1. The nutrient solution contains: soluble starch, dihydrogen phosphate, peptone, glucose, calcium chloride, B complex vitamins, and mushroom extract; The nutrient matrix contains: soluble starch, dihydrogen phosphate, peptone, glucose, calcium chloride, B complex vitamins, lentinan, wheat bran powder, and guar gum; The fungi include at least one of Florida oyster mushroom, elm oyster mushroom, Ganoderma lucidum, king oyster mushroom, enoki mushroom, or shiitake mushroom.

2. The method according to claim 1, characterized in that, The culture temperature is 25-35℃.

3. A fungal mycelium aerogel cushioning material, characterized in that, Prepared by the method described in any one of claims 1-2.

4. The method of any one of claims 1-2, or the fungal mycelium aerogel cushioning material of claim 3, in the preparation of home sofas, car seats, or clothing and footwear.

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