床旁诊断微流控芯片的制备方法

By designing a bedside diagnostic microfluidic chip with a fixed reaction volume and mixing channel, combined with active drive and superhydrophobic channels, the problems of poor detection repeatability and complex production in the prior art are solved, and multi-item detection and high sensitivity detection effects are achieved.

CN118080035BActive Publication Date: 2026-07-17DIYALAB ZJG BIOTECH CO LTD

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DIYALAB ZJG BIOTECH CO LTD
Filing Date
2018-12-13
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing POCT testing technologies for microfluidic chips suffer from problems such as poor repeatability, complex manufacturing processes, and inability to perform multi-item testing. In particular, active chips are difficult to package and passive chips have high randomness in testing.

Method used

A bedside diagnostic microfluidic chip was designed, which uses a reaction cell with a fixed reaction volume and a mixing channel. Combined with an active driving method, a passive flow blocking valve is formed using a superhydrophobic channel. Furthermore, multiple capture antibodies are coated in the detection channel to achieve precise control of samples and reagents and multi-item detection.

Benefits of technology

It improves the repeatability and sensitivity of detection, simplifies chip manufacturing processes, enables multi-item joint detection, and enhances the specificity and controllability of detection.

✦ Generated by Eureka AI based on patent content.

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Abstract

本发明公开了一种床旁诊断微流控芯片的制备方法,包括以下步骤:对芯片表面材料进行超亲水改性,之后将微流控芯片表面喷涂一层封闭液进行表面封闭,然后将芯片进行烘干处理;在凹槽微结构上覆盖超疏水试剂,对芯片中凹槽微结构的表面进行超疏水改性,然后将芯片进行烘干处理,得到超疏水管道;将荧光微球标记试剂添加至芯片基板中的混匀管道中,然后进行烘干,使荧光微球标记试剂干燥于芯片中并包被在第一微米柱上;将捕获抗体试剂包被于芯片基板的检测管道中,然后进行烘干,使捕获抗体固定于第二微米柱上;将芯片盖板与芯片基板进行组装并键合,得到床旁诊断微流控芯片。
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