A method for removing endotoxin from alginate oligosaccharide and alginate oligosaccharide with endotoxin removed

Abstract

The present application relates to a method for removing endotoxin from alginate oligosaccharide and alginate oligosaccharide with endotoxin removed, aiming to provide a method for efficiently removing endotoxin from alginate oligosaccharide with simple operation and easy industrialization. The technical points are: insoluble particles are removed by centrifugation, mixed Lewis acid is added for synergistic treatment, the treated solution is acidified to form a precipitate, the precipitate is washed with deionized water, an alkaline solution is added to dissolve the precipitate and filter, the filtrate is adsorbed by an adsorption medium, then alginate oligosaccharide is eluted with an organic solvent, and the alginate oligosaccharide with endotoxin removed is obtained after drying. The present application removes endotoxin from alginate oligosaccharide by using mixed Lewis acid synergistic treatment and adsorption by an adsorbent, no impurities are introduced during the preparation process, the endotoxin content in the obtained alginate oligosaccharide is low, the operation is simple, and the preparation process is suitable for industrial production.

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