A moisturizing anti-wrinkle mask serum

Abstract

The present application provides a kind of moisturizing anti-wrinkle mask essence, the essence simultaneously contains black ganoderma fermentation extract and black truffle extract, the black truffle extract is extracted by heating reflux with black truffle first for 2-3 hours with propylene glycol and water, after extracting 2-3 times, combined extract is concentrated by rotary evaporation under reduced pressure, then activated carbon is added, after stirring extraction for 10-30 min, it is placed, centrifuged, the supernatant is concentrated by rotary evaporation under reduced pressure to obtain black truffle extract;The black ganoderma fermentation extract is extracted by ultrasonic extraction after being crushed with black ganoderma and adding water for 20-40 min, and the filtrate is obtained by filtering out the residue, and the black ganoderma extract is obtained;Black ganoderma extract and black ganoderma mycelium are inoculated in sterilized seed culture medium to ferment for 7-10 days to obtain fermentation liquor, the fermentation liquor is sterilized, cooled, filtered to obtain black ganoderma fermentation liquor.The mask essence has good moisturizing and anti-wrinkle effect.

Description

Technical Field

[0001] This invention relates to the field of facial mask essences, specifically to a moisturizing and anti-wrinkle facial mask essence. Background Technology

[0002] Facial mask essence is a subcategory of skincare products used for beauty and skin care.

[0003] Black truffles (Latin name Tuber sinense, scientific name *Tuber sinense*) are a type of wild edible fungus that grows underground and has a rugged appearance. Black truffles have multiple uses in skincare products. They contain various natural moisturizing factors that can penetrate deep into the skin to provide long-lasting hydration, improve dryness, and increase skin elasticity. The rich vitamin C and polyphenols in black truffles give them strong antioxidant properties, helping the skin resist free radical damage, prevent skin aging, and reduce pigmentation, thus playing an anti-aging role. Furthermore, the polysaccharides and flavonoids in black truffles have certain anti-inflammatory effects, effectively eliminating skin redness, swelling, and allergies. Existing technologies include research on black truffle extracts and extraction methods, which are applied in the skincare field, primarily for moisturizing. For example, patent CN116077383A discloses a black truffle extract, its extraction process, and its applications.

[0004] Reishi mushroom (scientific name: *Ganoderma lucidum* (Curtis) P. Karst.) is a fungus belonging to the Ganodermataceae family and the *Ganoderma* genus. Reishi fermentation liquid is a functional liquid produced through microbial metabolism using *Ganoderma* strains as fermenting microorganisms under specific conditions. Reishi fermentation liquid contains various alkaloids and polyphenols, possessing excellent antioxidant properties. It can scavenge free radicals in the body, reduce the damage of oxidative stress to the human body, and delay aging. CN113832193A discloses a two-way fermentation liquid of *Ganoderma lucidum* and *Dendrobium*, a fermentation process, and the application of the fermentation liquid. Through two-way fermentation technology, the *Ganoderma lucidum* and *Dendrobium* two-way fermentation liquid obtained by two-way fermentation of *Ganoderma lucidum* and *Dendrobium* exhibits superior DPPH-clearing ability compared to *Dendrobium* extract, *Ganoderma lucidum* fermentation liquid, and *Ganoderma lucidum* and *Dendrobium* fermentation liquid without two-way fermentation. The fermentation liquid also has good anti-inflammatory effects, and masks made from the fermentation liquid have certain effects in moisturizing, hydrating, and whitening.

[0005] There are currently no products that combine black truffle extract and Ganoderma lucidum fermentation liquid in the same face mask. Summary of the Invention

[0006] To address the shortcomings of existing technologies, the purpose of this invention is to develop a moisturizing and anti-wrinkle facial mask essence that has good moisturizing and anti-wrinkle effects. Further research will be conducted on the raw materials and extraction processes of this mask essence, improving the process to enhance its functionality and improve its effectiveness after use.

[0007] To achieve the objective of this invention, the technical solution is as follows:

[0008] A moisturizing and anti-wrinkle facial mask essence includes deionized water. The essence contains both black Ganoderma lucidum fermentation extract and black truffle extract. The black truffle extract is obtained by first extracting black truffle with propylene glycol and water under reflux for 2-3 hours, then extracting with purified water under reflux for 2-3 hours, evaporating the combined extracts, concentrating under reduced pressure, adding activated carbon, ultrasonically extracting for 10-30 minutes, centrifuging, and then concentrating the supernatant under reduced pressure to obtain the black truffle extract. The black Ganoderma lucidum fermentation extract is obtained by crushing black Ganoderma lucidum, adding water, ultrasonically extracting for 4-6 hours, filtering to remove the residue, and obtaining a black Ganoderma lucidum extract. The black Ganoderma lucidum extract and black Ganoderma lucidum mycelium are inoculated into a sterilized seed culture medium and fermented for 7-10 days at 25℃-30℃ and pH 6.8-7.2 to obtain a fermentation broth. The fermentation broth is sterilized, cooled, and filtered to obtain the black Ganoderma lucidum fermentation broth.

[0009] Preferably, the moisturizing and anti-wrinkle mask essence comprises the following ingredients in parts by weight:

[0010] Component A: 80-90 parts deionized water; 0.05-0.1 parts disodium EDTA; 0.1-0.2 parts carbomer; 2-6 parts glycerol; 1-3 parts panthenol;

[0011] Component B: Triethanolamine 0.1-0.3 parts; Deionized water 1-3 parts;

[0012] Component C: 0.1-1 part of p-hydroxyacetophenone; 1-5 parts of butanediol;

[0013] Component D: Sodium hyaluronate 1-3 parts; water-soluble ceramide 1-3 parts; Ganoderma lucidum fermented extract 1-3 parts; arginine / lysine polypeptide 0.1-2 parts; Truffle extract 0.2-1 part; Eyepep035S polypeptide complex 0.1-1 part; palmitoyl tripeptide-5 0.1-1 part; recombinant type III humanized collagen ACNO.1 0.001-0.005 parts; conozoin polypeptide complex 0.001-0.005 parts;

[0014] Component E: 0.5-1 part, Component E includes one or more of capryloyl hydroxamic acid, ethylhexylglycerin, propylene glycol or 1,2-hexanediol;

[0015] Component F: 0.1-0.3 parts of hydrogenated castor oil.

[0016] The slash " / " means "and" or "or".

[0017] Preferably, component F also includes a small amount of fragrance, in an amount of 0.01-0.02 parts.

[0018] Preferably, the extraction method for the *Ichthyophthirius multifiliis* extract is as follows:

[0019] (1) Crush and sieve the dried black truffles to select 80-100 mesh black truffle powder;

[0020] (2) Add 500-600g of black truffle powder to a mixture of 2.5-3L of purified water and propylene glycol, wherein the volume ratio of water to propylene glycol in the mixture is (1-1.5):1. Heat and reflux for 2-3 hours, then centrifuge to obtain the extract.

[0021] (3) Then, under the same conditions, repeat the extraction 1-2 times, filter, and combine the extracts;

[0022] (3) The combined extract was concentrated to 1.5-2L by rotary evaporation under reduced pressure. Then, 10-20g of activated carbon was added. After adding activated carbon, the concentrate was stirred and extracted for 10-30 minutes, and then allowed to stand for 12-24 hours. Then, the supernatant was collected by centrifugation.

[0023] (5) The supernatant was then concentrated to 400-500 mL by rotary evaporation under reduced pressure to obtain the extract of Black Truffle.

[0024] Preferably, the black Ganoderma lucidum fermented extract is prepared by the following fermentation method:

[0025] (1) Ganoderma lucidum powder is crushed, sieved, and 20-30 mesh black Ganoderma lucidum powder is selected. Water is added and mixed. The weight ratio of black Ganoderma lucidum powder to water is (5-15):60 to obtain a mixture.

[0026] (2) After ultrasonic extraction of the mixture for 20-40 minutes, filter to remove the residue. Concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum extract. Take the black Ganoderma lucidum extract and black Ganoderma lucidum mycelium and inoculate them into a sterilized seed culture medium and ferment them in a fermenter. The inoculation amount of the black Ganoderma lucidum extract in the culture medium is 5%-10%, and the mass of the black Ganoderma lucidum mycelium is 0.1-0.5 times the mass of the black Ganoderma lucidum extract. Ferment for 7-10 days under the conditions of 25℃-30℃, 160-240r / min, and pH 6.8-7.2. After fermentation, sterilize at 120℃-130℃ for 10-20 minutes, cool, filter through a 0.2-0.4μm filter membrane, and concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum fermentation liquid.

[0027] Black Ganoderma mycelium is the mycelium of fresh black Ganoderma.

[0028] More preferably, the seed culture medium comprises the following components and concentrations: glucose 30-40 g / L, yeast powder 2-5 g / L, peptone 2-8 g / L, magnesium sulfate heptahydrate 1-3 g / L, corn starch 5-10 g / L, and the remainder being deionized water.

[0029] A further preferred embodiment of the method for preparing the moisturizing and anti-wrinkle facial mask liquid includes the following steps:

[0030] (1) Mix and disperse each component of phase A, heat to 85℃-90℃, soak and swell, stir homogenously until there are no particles, and obtain phase A mixture;

[0031] (2) Stir the A phase mixture and cool it to 40℃-45℃, add the components of the B phase, stir to make the components evenly dispersed, and obtain the AB mixture;

[0032] (3) Add the pre-dissolved components of item C, item D, item E, and item F to the AB mixture in sequence, stir evenly, let stand for 24-48 hours, and then filter through a 300-400 mesh screen to obtain the moisturizing and anti-wrinkle mask essence.

[0033] Compared with the prior art, the advantages of the present invention are:

[0034] 1. Optimized ingredients: The various raw materials of the mask liquid of the present invention are formulated in a specific ratio to make the moisturizing and anti-wrinkle effects of the mask liquid better.

[0035] 2. The preparation methods of the black Ganoderma lucidum fermentation extract and black truffle extract in the mask liquid of the present invention are innovative, and more efficient process technology is adopted to make the effective ingredients in the mask liquid easier to be absorbed by the skin and have better effects.

[0036] 3. Effects of use: Improved in terms of user experience and skin comfort.

[0037] The detailed structure of the present invention will be further described below with reference to specific embodiments. Detailed Implementation

[0038] Example 1

[0039] A moisturizing and anti-wrinkle facial mask liquid comprises the following ingredients in percentage by weight as shown in Table 1:

[0040] Table 1. Ingredients and mass percentage of the moisturizing and anti-wrinkle mask liquid in Example 1

[0041]

[0042] The extract of *Tuber melanosporum* in component D was prepared using the following extraction method:

[0043] (1) Use a handheld portable grinder to grind the dried black truffles into 80-mesh black truffle powder;

[0044] (2) Weigh 500g of black truffle powder and add 2.5 L of purified water + propylene glycol mixture. The volume ratio of water to propylene glycol is 1:1 (L:L). Set the electric heating mantle to 200W and heat under reflux for 2 hours. Centrifuge to obtain the extract.

[0045] (3) Then, under the same conditions, the extraction was repeated once, filtered, and the two extracts were combined;

[0046] (4) Concentrate the extract to 2L under reduced pressure on a rotary evaporator, add 10g of activated carbon, stir the concentrated extract after adding activated carbon for 10 min, and let it stand overnight (for 12 hours); then centrifuge at 6500 r / min for 10 min and take the supernatant.

[0047] (5) The supernatant was concentrated under reduced pressure to 500 mL to obtain the tuber extract.

[0048] Black truffle extract has a pleasant aroma.

[0049] The black Ganoderma lucidum fermentation extract in component D was prepared by the following fermentation method:

[0050] (1) Select black Ganoderma lucidum, remove impurities, crush it, pass it through a 20-mesh sieve, add water and mix. The weight ratio of black Ganoderma lucidum powder to water is 15:60.

[0051] (2) After ultrasonic extraction of the mixture for 20-40 minutes, filter to remove the residue. Concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum extract. Take the black Ganoderma lucidum extract and Ganoderma lucidum mycelium and inoculate them into a sterilized seed culture medium in a fermenter. The inoculation amount of the black Ganoderma lucidum extract in the culture medium is 5%, and the mass of the black Ganoderma lucidum mycelium is 0.2 times the mass of the black Ganoderma lucidum extract. Ferment for 7 days under the conditions of 25℃, 160r / min and pH 7.0. After fermentation, sterilize at 121℃ for 10 minutes, cool, filter through a 0.2μm filter membrane, and concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum fermentation liquid.

[0052] The seed culture medium consists of the following components and concentrations: glucose 32 g / L, yeast extract 5 g / L, peptone 5 g / L, magnesium sulfate heptahydrate 1.0 g / L, corn starch 5 g / L, and the remainder is deionized water.

[0053] The recombinant type III humanized collagen ACNO.1 and conopod protein polypeptide complex in component D were purchased from Guangzhou Meimei Biopharmaceutical Co., Ltd.; the Eyepep035S polypeptide complex in component D was purchased from Zhejiang Paipai Biotechnology Co., Ltd.

[0054] The preparation method of the above-mentioned moisturizing and anti-wrinkle facial mask liquid includes the following steps:

[0055] (1) Mix and disperse each component of phase A, heat to 85°C, soak and swell, stir homogenously until there are no particles, and obtain phase A mixture;

[0056] (2) Stir the A phase mixture and cool it to 45°C. Add the components of the B phase and stir to make the components evenly dispersed to obtain the AB mixture.

[0057] (3) Add the pre-dissolved components of item C, item D, item E, and item F to the AB mixture in sequence, stir evenly, let stand for 24 hours, and then filter through a 300-400 mesh screen to obtain the moisturizing and anti-wrinkle mask essence.

[0058] The specific effects of the moisturizing and anti-wrinkle facial mask liquid obtained in Example 1 are shown in Table 4.

[0059] Example 2:

[0060] A moisturizing and anti-wrinkle facial mask liquid comprises the following ingredients in percentage by weight as shown in Table 2:

[0061] Table 2. Ingredients and mass percentage of the moisturizing and anti-wrinkle mask liquid in Example 2

[0062]

[0063] The extract of *Tuber nigricans* in component D was prepared using the following extraction method:

[0064] (1) Use a handheld portable grinder to grind the dried black truffles into 90-mesh black truffle powder;

[0065] (2) Weigh 500g of black truffle powder and add 2.8 L of purified water + propylene glycol mixture. The volume ratio of water to propylene glycol is 2:1 (L:L). Set the heating mantle to 200W and heat under reflux for 1.5h. Centrifuge to obtain the extract.

[0066] (3) Then, under the same conditions, the extraction was repeated once, filtered, and the two extracts were combined;

[0067] (4) Concentrate the extract to 2L under reduced pressure on a rotary evaporator, add 15g of activated carbon, stir the concentrated extract after adding activated carbon for 15min, and let it stand overnight (for 18 hours); then centrifuge at 6500 r / min for 15min and take the supernatant.

[0068] (5) The supernatant was concentrated under reduced pressure to 500 mL to obtain the tuber extract.

[0069] Black truffle extract has a pleasant aroma.

[0070] The black Ganoderma lucidum fermentation extract in component D was prepared by the following fermentation method:

[0071] (1) Select black Ganoderma lucidum, remove impurities, crush it, pass it through a 30-mesh sieve, add water and mix. The weight ratio of black Ganoderma lucidum powder to water is 10:60.

[0072] (2) After ultrasonic extraction of the mixture for 30 min, filter to remove the residue. Concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum extract. Take the black Ganoderma lucidum extract and Ganoderma lucidum mycelium and inoculate them into a sterilized seed culture medium in a fermenter. The inoculation amount of the black Ganoderma lucidum extract in the culture medium is 6%, and the mass of the black Ganoderma lucidum mycelium is 0.15 times the mass of the black Ganoderma lucidum extract. Ferment for 8 days under the conditions of 25℃, 160r / min and pH 7.0. After fermentation, sterilize at 121℃ for 5 minutes, cool, filter through a 0.3μm filter membrane, and concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum fermentation liquid.

[0073] The recombinant type III humanized collagen ACNO.1 and conopod protein polypeptide complex in component D were purchased from Guangzhou Meimei Biopharmaceutical Co., Ltd.; the Eyepep035S polypeptide complex in component D was purchased from Zhejiang Paipai Biotechnology Co., Ltd.

[0074] The preparation method of the above-mentioned moisturizing and anti-wrinkle facial mask liquid includes the following steps:

[0075] (1) Mix and disperse each component of phase A, heat to 90°C, soak and swell, stir homogenously until there are no particles, and obtain phase A mixture;

[0076] (2) Stir the A phase mixture and cool it to 40°C. Add the components of the B phase and stir to make the components evenly dispersed to obtain the AB mixture.

[0077] (3) Add the pre-dissolved components of item C, item D, item E, and item F to the mixture of AB in sequence, stir evenly, let stand for 36 hours, and then filter through a 300-400 mesh screen to obtain the moisturizing and anti-wrinkle mask essence.

[0078] The specific effects of the moisturizing and anti-wrinkle facial mask liquid obtained in Example 2 are shown in Table 4.

[0079] Example 3:

[0080] A moisturizing and anti-wrinkle facial mask liquid comprises the following ingredients in percentage by weight as shown in Table 3:

[0081] Table 3. Ingredients and mass percentage of the moisturizing and anti-wrinkle mask liquid in Example 3

[0082]

[0083] The extraction methods for *Ichthyophthirius multifiliis* extract and *Ganoderma lucidum* fermentation extract were the same as in Example 1, and the preparation method for the essence was also the same as in Example 1. Specific experimental data on the effects are shown in Table 4.

[0084] Comparative Example 1: Regular face mask - without black truffle or Ganoderma lucidum ingredients

[0085] The rest of the formula is the same as in Example 1, but it does not contain *Ichthyophthirius multifiliis* extract or *Ganoderma lucidum* fermentation extract. The preparation method of the mask liquid is the same as in Example 1. See Table 4 for specific experimental data on the effects.

[0086] Results: The liquid in the mask prepared in Comparative Example 2 was refreshing. For specific experimental data, please see Table 4.

[0087] Comparative Example 2: Black Truffle Mask - No Ganoderma Lucidum Ingredients

[0088] This case study describes a black truffle extract and its extraction process. The rest of the formula is the same as in Example 1. The extraction method of the black truffle extract and the preparation method of the mask liquid are the same as in Example 1. The difference is that the mask formula contains 0.5% black truffle extract, but does not contain Ganoderma lucidum fermentation liquid.

[0089] Results: The liquid in the mask prepared in Comparative Example 2 was refreshing. For specific experimental data, please see Table 4.

[0090] Comparative Example 3: Reishi Mushroom Mask - No Black Truffle Ingredients

[0091] This case provides a black Ganoderma lucidum fermented extract and its fermentation process. The rest of the formula is the same as in Example 1. The extraction method of the black Ganoderma lucidum fermented extract and the preparation method of the mask liquid are the same as in Example 1. The difference is that no black truffle extract is added to the mask formula, but it contains 1% black Ganoderma lucidum fermented extract.

[0092] Results: The liquid mask prepared in Comparative Example 3 was more viscous than that in Comparative Example 1. For specific experimental data, please see Table 4.

[0093] Comparative Example 4:

[0094] The preparation process and formulation of the mask liquid in this case are the same as in Example 1; the difference is that a different extraction process for black truffle extract and a fermentation process for black Ganoderma lucidum extract are used.

[0095] The specific extraction process of the black truffle extract described in this comparative example is as follows:

[0096] (1) Grind the dried black truffles into 80-mesh coarse powder using a handheld portable grinder;

[0097] (2) Weigh 500g of black truffle powder, add 2.5 L of purified water, set the electric heating mantle to 200W, heat and reflux for 2 hours, and filter the extract with clean gauze.

[0098] (3) Repeat the extraction once under the same conditions, filter, and combine the two extracts;

[0099] (4) The combined extract was concentrated to 500 mL under reduced pressure on a rotary evaporator to obtain a sample with a light aroma.

[0100] The specific fermentation process of the black Ganoderma lucidum fermented extract described in this comparative example is as follows:

[0101] (1) Remove impurities from black Ganoderma lucidum, wash it clean, select black Ganoderma lucidum, crush it, pass it through a 20-mesh sieve, add ethanol solution and mix to obtain a mixture (the weight ratio of black Ganoderma lucidum powder to ethanol solution is 15:60), wherein the mass concentration of ethanol solution is 70%;

[0102] (2) Heat the mixture to 60℃ and extract for 2 hours, filter, take the residue and extract again 2-3 times, filter, combine the two extracts, and concentrate under reduced pressure at 60℃ to 1000mL to obtain Ganoderma lucidum extract.

[0103] (3) Take 100mL of Ganoderma lucidum extract, 5mL of Lactococcus lactis culture (108CFU / mL), 5g of MRS broth culture medium and 400mL of deionized water and mix them. Ferment at 40℃ for 7 days. After fermentation, sterilize the fermentation liquid at 121℃ for 20min. Then centrifuge at a centrifuge radius of 15cm and a speed of 3000rpm for 30min. Take the supernatant after centrifugation, which is the black Ganoderma lucidum fermentation extract.

[0104] Results: This formula is also a mask containing black truffle extract and black Ganoderma lucidum fermented extract, but the specific extraction method is different. The moisturizing and anti-wrinkle effects are worse than those in Example 1. Please see Table 4 for specific experimental data.

[0105] Conclusion of the experiment:

[0106] 1. Moisturizing Efficacy: The moisturizing effects of various facial masks and saline solution were evaluated according to the "QBT4256-2011 Guidelines for Evaluating the Moisturizing Efficacy of Cosmetics". A 4cm × 4cm test area was selected on healthy skin on the inner forearm, and the tested skincare product was applied at a concentration of 2.0 ± 0.1 mg / cm². 2The product was applied as a single patch. Skin moisture content in each test area was measured before and after application using a capacitive skin moisture meter, with a 1-hour time interval. Ten volunteers aged 20 to 40 years participated in the test. Two areas were selected for each sample from each subject, and skin moisture in each area was measured three times. The average value was taken. The test results for each embodiment and comparative example are shown in Table 4 below. Higher moisture content indicates better moisturizing effect.

[0107] Table 4 Product Skin Moisture Detection Data ( (n=10)

[0108]

[0109] Note: Compared with control skin* P <0.05,** P <0.01.

[0110] As can be seen from the above data, the black truffle + Ganoderma lucidum masks in Examples 1, 2, and 3 of this invention have good moisturizing effects. The skin's moisture content within 1-18 hours after using the mask was significantly higher than before use, and also significantly higher than the skin moisture content of Comparative Examples 1, 2, 3, and 4. This demonstrates that the moisturizing and anti-wrinkle mask liquid of this invention has good moisturizing effects and a long-lasting moisturizing effect.

[0111] 2. Anti-wrinkle efficacy: The left and right eye areas of 50 participants were selected as the test areas. The participants were divided into 5 groups: four sample groups and one control group. Participants applied the product to the same test area (washed with water) morning and evening using the same method. The skin texture of the participants was measured after different application times using the MicroSkin II multifunctional dermoscopy image analysis system. The average roughness of eye wrinkles and the rate of change of skin elasticity R² value were measured for each group. R² value refers to the ratio of skin rebound without negative pressure to the maximum stretch under negative pressure. Based on R² change rate = (R² value - initial R² value) / initial R² value, the R² change rate after the corresponding time can be calculated. The R² change rate reflects the change in skin elasticity over time in the test area during the test period. The results are shown in Table 5. A larger R² change rate indicates a more significant change in the skin and a better effect.

[0112] Table 5 Data Table of Product Skin Anti-wrinkle Function Test ( (n=10)

[0113]

[0114] Note: Compared with control skin* P <0.05,**P <0.01.

[0115] As can be seen from the above data, the black truffle + Ganoderma lucidum facial mask liquid in Examples 1, 2, and 3 of this invention has a good anti-wrinkle effect. Comparative data from before use, 2 weeks of use, and 4 weeks of use show a significant improvement in skin roughness and a large change in the skin elasticity R2 value, which is significantly better than the facial mask liquids in the comparative examples. The anti-wrinkle effect is: Example 1 (Examples 2 and 3) > Comparative Example 4 > Comparative Example 2 > Comparative Example 3 > Comparative Example 1. This proves that the moisturizing and anti-wrinkle facial mask liquid of this invention has a good anti-wrinkle effect.

[0116] The above description is a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the technical scope disclosed in the present invention, based on the technical solution and concept of the present invention, should be covered within the scope of protection of the claims of the present invention.

Claims

1. A moisturizing and anti-wrinkle facial mask essence, comprising deionized water, characterized in that, The essence contains both black Ganoderma lucidum fermentation extract and black truffle extract. The black truffle extract is obtained by extracting black truffle powder with propylene glycol and water under reflux for 2-3 hours, repeating the extraction 2-3 times. The extracts are then combined and concentrated under reduced pressure by rotary evaporation. Activated carbon is added, and the mixture is stirred and extracted for another 10-30 minutes. After standing and centrifugation, the supernatant is concentrated under reduced pressure by rotary evaporation to obtain the black truffle extract. The black Ganoderma lucidum fermentation extract is obtained by crushing black Ganoderma lucidum, adding water, and ultrasonically extracting for 20-40 minutes. The residue is then removed by filtration to obtain a black Ganoderma lucidum extract. The black Ganoderma lucidum extract and black Ganoderma lucidum mycelium are inoculated into a sterilized seed culture medium and fermented for 7-10 days at 25℃-30℃ and pH 6.8-7.2 to obtain a fermentation broth. The fermentation broth is then sterilized, cooled, and filtered to obtain the black Ganoderma lucidum fermentation broth. The moisturizing and anti-wrinkle facial mask essence comprises the following ingredients in parts by weight: Component A: 80-90 parts deionized water; 0.05-0.1 parts disodium EDTA; 0.1-0.2 parts carbomer; 2-6 parts glycerol; 1-3 parts panthenol; Component B: Triethanolamine 0.1-0.3 parts; Deionized water 1-3 parts; Component C: 0.1-1 part of p-hydroxyacetophenone; 1-5 parts of butanediol; Component D: Sodium hyaluronate 1-3 parts; water-soluble ceramide 1-3 parts; Ganoderma lucidum fermented extract 1-3 parts; arginine / lysine polypeptide 0.1-2 parts; Truffle extract 0.2-1 part; Eyepep035S polypeptide complex 0.1-1 part; palmitoyl tripeptide-5 0.1-1 part; Recombinant type III humanized collagen ACNO.1 0.001-0.005 parts; conopod protein polypeptide complex 0.001-0.005 parts; Component E: 0.5-1 part, Component E includes one or more of capryloyl hydroxamic acid, ethylhexylglycerin, propylene glycol or 1,2-hexanediol; Component F: 0.1-0.3 parts of hydrogenated castor oil.

2. The moisturizing and anti-wrinkle facial mask essence according to claim 1, characterized in that, Component F also includes fragrance.

3. The moisturizing and anti-wrinkle facial mask essence according to claim 1 or 2, characterized in that, The extraction method of the *Tuber nigricans* extract is as follows: (1) Crush and sieve the dried black truffles to select 80-100 mesh black truffle powder; (2) Add 500-600g of black truffle powder to a mixture of 2.5-3L of purified water and propylene glycol, wherein the volume ratio of water to propylene glycol in the mixture is (1-1.5):

1. Heat and reflux for 2-3 hours, then centrifuge to obtain the extract. (3) Then, under the same conditions, repeat the extraction 1-2 times, filter, and combine the extracts; (4) The combined extract is concentrated to 1.5L-2L by rotary evaporation under reduced pressure. Then, 10-20g of activated carbon is added. After adding activated carbon, the concentrate is stirred and extracted for 10-30min and then allowed to stand for 12-24 hours. Centrifuge again and collect the supernatant; (5) The supernatant was then concentrated to 400-500 mL by rotary evaporation under reduced pressure to obtain the extract of Black Truffle.

4. The moisturizing and anti-wrinkle facial mask essence according to claim 1 or 2, characterized in that, The black Ganoderma lucidum fermented extract was prepared by the following fermentation method: (1) Crush black Ganoderma lucidum, sieve it, select 20-30 mesh black Ganoderma lucidum powder, add water and mix. The weight ratio of black Ganoderma lucidum powder to water is (5-15):60 to obtain a mixture; (2) After ultrasonic extraction of the mixture for 20-40 minutes, filter to remove the residue. Concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum extract. Take the black Ganoderma lucidum extract and black Ganoderma lucidum mycelium and inoculate them into a sterilized seed culture medium and ferment them in a fermenter. The inoculation amount of the black Ganoderma lucidum extract in the culture medium is 5%-10%, and the mass of the black Ganoderma lucidum mycelium is 0.1-0.5 times the mass of the black Ganoderma lucidum extract. Ferment for 7-10 days under the conditions of 25℃-30℃, 160-240r / min, and pH 6.8-7.

2. After fermentation, sterilize at 120℃-130℃ for 10-20 minutes, cool, filter through a 0.2-0.4μm filter membrane, and concentrate the filtrate to the same mass as the weighed black Ganoderma lucidum powder to obtain black Ganoderma lucidum fermentation liquid.

5. The moisturizing and anti-wrinkle facial mask essence according to claim 4, characterized in that, The seed culture medium consists of the following components and concentrations: glucose 30-40 g / L, yeast extract 2-5 g / L, peptone 2-8 g / L, magnesium sulfate heptahydrate 1-3 g / L, corn starch 5-10 g / L, and the remainder is deionized water.

6. The moisturizing and anti-wrinkle facial mask essence according to claim 1 or 2, characterized in that, The preparation method of the moisturizing and anti-wrinkle facial mask liquid includes the following steps: (1) Mix and disperse each component of phase A, heat to 85℃-90℃, soak and swell, stir homogenously until there are no particles, and obtain phase A mixture; (2) Stir the A phase mixture and cool it to 40℃-45℃, add the components of the B phase, stir to make the components evenly dispersed, and obtain the AB mixture; (3) Add the pre-dissolved components of item C, item D, item E, and item F to the AB mixture in sequence, stir evenly, let stand for 24-48 hours, and then filter through a 300-400 mesh screen to obtain the moisturizing and anti-wrinkle mask essence.

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