A kit for detecting mycobacterium tuberculosis specific cellular immune response

Abstract

This invention relates to the field of reagent kit technology and discloses a Mycobacterium tuberculosis-specific cellular immune response detection kit, comprising a box body with a lid hinged to the top. The box body has a storage hole and a placement slot inside. A top plate and a bottom plate are fixedly installed on the inner wall of the storage hole, and a reagent tube is rotatably installed on the inner wall of the storage hole, positioned between the top plate and the bottom plate. This invention utilizes the side edge of the lid hinge to abut against a pressure post, causing the pressure post to push an L-shaped plate towards a guide post. Because the end of the L-shaped plate abuts against the conical surface of a conical top block, the movement of the L-shaped plate can lift the conical top block upwards, causing it to extend beyond the upper surface of the bottom plate. This, in turn, lifts a slidingly mounted base in the placement hole upwards, causing the base to lift the reagent tube placed in the placement hole upwards, so that the top of the reagent tube extends beyond the upper surface of the top plate, facilitating the removal of the reagent tube.

Description

Technical Field

[0001] This invention relates to the field of reagent kit technology, and more particularly to a reagent kit for detecting Mycobacterium tuberculosis-specific cellular immune responses. Background Technology

[0002] Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis, causing significant harm. Although TB ​​is well-known in the medical community today, it still holds important epidemiological significance in some regions. TB continues to cause a large number of deaths and a significant disease burden worldwide. Therefore, the prevention and control of TB is of great scientific importance.

[0003] Current diagnostic methods for tuberculosis infection include tuberculin skin test, interferon-gamma release test, recombinant tuberculosis fusion protein skin test, tuberculosis-specific immunoglobulin IgG and IgM, Xpert MTB / RIF technology, and combined diagnosis using the above methods.

[0004] With the continuous improvement of detection technology, Mycobacterium tuberculosis-specific cell-mediated immune response detection kits have begun to appear and be promoted for use in the market. These kits typically include reagent bottles, pipettes, reaction tubes, and detection plates.

[0005] An existing Mycobacterium tuberculosis-specific cellular immune response detection kit (publication number: CN215206131U) has at least the following drawbacks: The aforementioned patent places the reagent tubes required for the reaction detection inside the box, making it easy to carry the box externally; however, because multiple reagent tubes are arranged side by side, it is easy for the testing personnel to have difficulty picking up the required reagent tube when picking up a single reagent tube due to the small gap between adjacent reagent tubes. Summary of the Invention

[0006] The purpose of this invention is to address the shortcomings of existing technologies by proposing a Mycobacterium tuberculosis-specific cellular immune response detection kit.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A kit for detecting Mycobacterium tuberculosis-specific cellular immune response includes a box body with a lid hinged to its top. The box body has a storage hole and a placement slot inside. A top plate and a bottom plate are fixedly installed on the inner wall of the storage hole. A reagent cylinder is rotatably installed on the inner wall of the storage hole, positioned between the top and bottom plates. Multiple placement holes are equidistantly spaced along the circumference of the top of the reagent cylinder. Corresponding through holes are formed on the upper surfaces of the bottom and top plates. A guide post is fixedly installed on the bottom wall of the box body near the through holes. A conical top block is fitted onto the outer surface of the guide post. A lifting mechanism for pushing the conical top block upwards is provided between the lid and the box body.

[0009] As a further embodiment of the present invention, the lifting mechanism includes an L-shaped groove inside the box body, an L-shaped plate slidably mounted on the inner wall of the L-shaped groove, one end of the L-shaped plate near the guide post abutting against the conical surface of the conical top block, a pressure post fixedly mounted on the outer surface of the other end of the L-shaped plate, the side of the box cover after it is fully opened abutting against the end of the pressure post, and a reset assembly is installed between the L-shaped plate and the bottom of the box body.

[0010] As a further embodiment of the present invention, the reset assembly includes a fixing block fixedly installed on the bottom wall of the box body, a rectangular groove is provided through the bottom of the L-shaped plate, the fixing block is slidably installed on the inner wall of the rectangular groove, a guide rod is fixedly installed between the inner walls at both ends of the rectangular groove, a reset spring is sleeved on the outer surface of the guide rod, and the reset spring is disposed between the fixing block and the inner wall of the rectangular groove at the end away from the guide post.

[0011] As a further embodiment of the present invention, a sliding groove is provided on the lower surface of the top plate near the through hole, a cover plate is slidably installed on the inner wall of the sliding groove, two wedge-shaped blocks are fixedly installed on the upper surface of the cover plate, two pressure rods adapted to the wedge-shaped blocks are fixedly installed on the inner side of the box cover, and a tension spring is fixedly installed between the cover plate and the inner wall of the sliding groove.

[0012] As a further embodiment of the present invention, a fixing mechanism is installed at the bottom of the box body. The fixing mechanism includes four fixing tubes that are evenly fixedly installed at the bottom of the box body. Each of the four fixing tubes has a suction cup that is connected to its interior fixedly installed at its bottom end. A conduit that is connected to each other is fixedly installed between the four fixing tubes. An air suction component is installed between the bottom of the box body and the L-shaped plate.

[0013] As a further embodiment of the present invention, the air intake assembly includes an air intake pipe fixedly installed at the bottom of the box body. The end of the air intake pipe near the L-shaped plate is a closed structure. A piston is slidably installed on the inner wall of the air intake pipe. A connecting rod is fixedly installed on the lower surface of the L-shaped plate. The connecting rod passes through the closed end of the air intake pipe and is fixedly installed on the outer surface of the piston. The conduit is connected to the interior of the closed end of the air intake pipe.

[0014] As a further embodiment of the present invention, a base is slidably mounted on the inner wall of the placement hole, and the bottom of the base has a conical structure.

[0015] As a further embodiment of the present invention, a knob is fixedly installed at the rotation center of the reagent cylinder.

[0016] The beneficial effects of this invention are as follows:

[0017] 1. The side of the hinge of the box cover abuts against the pressure column, causing the pressure column to push the L-shaped plate in the direction of the guide column. Since the end of the L-shaped plate abuts against the conical surface of the conical top block, the movement of the L-shaped plate can lift the conical top block upward, so that the conical top block extends out of the upper surface of the bottom plate. The conical top block lifts the bottom support that is slidably installed in the placement hole upward, and the bottom support lifts the reagent tube placed in the placement hole upward, so that the top of the reagent tube extends out of the upper surface of the top plate, making it convenient to take out the reagent tube.

[0018] 2. As the lid moves the L-shaped plate via the pressure column, the L-shaped plate moves the piston via the connecting rod. The piston then draws out the air between the suction cup and the table through the conduit, allowing the suction cup to adhere to the table and preventing the box from moving during use, which would affect the detection effect of Mycobacterium tuberculosis specific cellular immune response. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of a Mycobacterium tuberculosis-specific cell immune response detection kit proposed in this invention;

[0020] Figure 2 This is a schematic diagram of the bottom structure of a Mycobacterium tuberculosis-specific cellular immune response detection kit proposed in this invention;

[0021] Figure 3 This is a schematic diagram of the internal structure of the kit for detecting Mycobacterium tuberculosis-specific cellular immune responses proposed in this invention.

[0022] Figure 4 This is a schematic diagram of the storage well structure of a Mycobacterium tuberculosis-specific cell immunoreaction detection kit proposed in this invention;

[0023] Figure 5 This is a schematic diagram of the L-shaped plate structure of a Mycobacterium tuberculosis-specific cellular immune response detection kit proposed in this invention;

[0024] Figure 6 This is a schematic diagram of the reagent cartridge structure of a Mycobacterium tuberculosis-specific cellular immune response detection kit proposed in this invention;

[0025] Figure 7This is a schematic diagram of the top plate structure of a Mycobacterium tuberculosis-specific cellular immune response detection kit proposed in this invention;

[0026] Figure 8 This is a schematic diagram of the cover plate structure of a Mycobacterium tuberculosis-specific cellular immune response detection kit proposed in this invention;

[0027] Figure 9 This is a schematic diagram of the internal structure of the inhalation tube of a Mycobacterium tuberculosis-specific cellular immune response detection kit proposed in this invention.

[0028] In the diagram: 1. Box body; 2. Box lid; 3. Storage hole; 4. Placement slot; 5. Top plate; 6. Reagent cylinder; 7. Bottom plate; 8. Placement hole; 9. Base support; 10. Guide post; 11. Conical top block; 12. L-shaped plate; 13. Pressure post; 14. Fixing block; 15. Guide rod; 16. Return spring; 17. Knob; 18. Cover plate; 19. Wedge block; 20. Pressure rod; 21. Tension spring; 22. Fixing tube; 23. Suction cup; 24. Guide tube; 25. Suction tube; 26. Connecting rod; 27. Piston. Detailed Implementation

[0029] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0030] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0031] See attached document Figure 1 -Appendix Figure 9A kit for detecting Mycobacterium tuberculosis-specific cellular immune responses includes a box body 1, with a lid 2 hinged to the top of the box body 1 and locked to the box body 1 by a snap-fit ​​connection; the box body 1 has a storage hole 3 and a placement slot 4 inside, a top plate 5 and a bottom plate 7 are fixedly installed on the inner wall of the storage hole 3, a reagent cylinder 6 is rotatably installed on the inner wall of the storage hole 3, the reagent cylinder 6 is positioned between the top plate 5 and the bottom plate 7, the reagent cylinder 6 is rotatably installed via a damping shaft to prevent the reagent cylinder 6 from rotating arbitrarily, multiple placement holes 8 are equidistantly opened in the circumferential direction at the top of the reagent cylinder 6, and corresponding through holes are opened through the upper surfaces of the bottom plate 7 and the top plate 5, and a guide is fixedly installed on the bottom wall of the box body 1 near the through holes. A conical top block 11 is fitted on the outer surface of the column 10 and the guide column 10. A lifting mechanism is provided between the cover 2 and the box body 1 to push the conical top block 11 upward. The lifting mechanism includes an L-shaped groove opened inside the box body 1. An L-shaped plate 12 is slidably installed on the inner wall of the L-shaped groove. One end of the L-shaped plate 12 near the guide column 10 abuts against the conical surface of the conical top block 11. A pressure column 13 is fixedly installed on the outer surface of the other end of the L-shaped plate 12. The side of the cover 2 after it is fully opened abuts against the end of the pressure column 13. A reset component is installed between the L-shaped plate 12 and the bottom of the box body 1. A base 9 is slidably installed on the inner wall of the placement hole 8. The bottom of the base 9 has a conical structure. A knob 17 is fixedly installed at the rotation center of the reagent cylinder 6.

[0032] When in use, open the lid 2 so that the side of the hinge of the lid 2 abuts against the pressure post 13, so that the pressure post 13 pushes the L-shaped plate 12 in the direction of the guide post 10. Since the end of the L-shaped plate 12 abuts against the conical surface of the conical top block 11, the movement of the L-shaped plate 12 can lift the conical top block 11 upward, so that the conical top block 11 extends out of the upper surface of the bottom plate 7, so that the conical top block 11 lifts the bottom support 9 that is slidably installed in the placement hole 8 upward, so that the bottom support 9 lifts the reagent tube placed in the placement hole 8 upward, so that the top of the reagent tube extends out of the upper surface of the top plate 5, making it convenient to take out the reagent tube.

[0033] In this embodiment, the reset assembly includes a fixing block 14 fixedly installed on the bottom wall of the box 1. A rectangular groove is opened through the bottom of the L-shaped plate 12. The fixing block 14 is slidably installed on the inner wall of the rectangular groove. A guide rod 15 is fixedly installed between the inner walls at both ends of the rectangular groove. A reset spring 16 is sleeved on the outer surface of the guide rod 15. The reset spring 16 is disposed between the fixing block 14 and the inner wall of the rectangular groove at the end away from the guide post 10. The reset spring 16 is used to push the L-shaped plate 12 to reset.

[0034] In this embodiment, a groove is provided on the lower surface of the top plate 5 near the through hole. A cover plate 18 is slidably installed on the inner wall of the groove. Two wedge blocks 19 are fixedly installed on the upper surface of the cover plate 18. Two pressure rods 20 that are adapted to the wedge blocks 19 are fixedly installed on the inner side of the box cover 2. A tension spring 21 is fixedly installed between the cover plate 18 and the inner wall of the groove.

[0035] When the lid 2 is closed, the pressure rod 20 installed on the inside of the lid 2 will abut against the inclined surface of the wedge block 19, so that the pressure rod 20 pushes the cover plate 18 toward the through hole through the inclined surface of the wedge block 19, so that the cover plate 18 seals the through hole on the top plate 5, preventing the reagent tube from shaking in the reagent cylinder 6 and affecting the transfer of the reagent tube.

[0036] In this embodiment, a fixing mechanism is installed at the bottom of the box body 1. The fixing mechanism includes four fixing tubes 22 that are evenly fixedly installed at the bottom of the box body 1. Each of the four fixing tubes 22 has a suction cup 23 that is connected to its interior fixedly installed at its bottom end. A conduit 24 that is connected to each other is fixedly installed between the four fixing tubes 22. An air suction assembly is installed between the bottom of the box body 1 and the L-shaped plate 12. The air suction assembly includes an air suction pipe 25 that is fixedly installed at the bottom of the box body 1. The end of the air suction pipe 25 near the L-shaped plate 12 is a closed structure. A piston 27 is slidably installed on the inner wall of the air suction pipe 25. A connecting rod 26 is fixedly installed on the lower surface of the L-shaped plate 12. The connecting rod 26 passes through the closed end of the air suction pipe 25 and is fixedly installed on the outer surface of the piston 27. The conduit 24 is connected to the interior of the closed end of the air suction pipe 25.

[0037] While the lid 2 pushes the L-shaped plate 12 to move via the pressure column 13, the L-shaped plate 12 drives the piston 27 to move via the connecting rod 26. This causes the piston 27 to extract the air between the suction cup 23 and the table through the conduit 24, allowing the suction cup 23 to adhere to the table and preventing the box 1 from moving during use, which would affect the detection effect of Mycobacterium tuberculosis specific cellular immune response.

[0038] From the above description, it can be seen that the above embodiments of the present invention achieve the following technical effects: When in use, the box cover 2 is opened, so that the side of the hinge of the box cover 2 abuts against the pressure column 13, so that the pressure column 13 pushes the L-shaped plate 12 in the direction of the guide column 10. Since the end of the L-shaped plate 12 abuts against the conical surface of the conical top block 11, the movement of the L-shaped plate 12 can push the conical top block 11 upward, so that the conical top block 11 extends out of the upper surface of the bottom plate 7, so that the conical top block 11 pushes the bottom support 9 slidably installed in the placement hole 8 upward, so that the bottom support 9 pushes the reagent tube placed in the placement hole 8 upward, so that the top of the reagent tube extends out of the upper surface of the top plate 5, making it convenient to take out the reagent tube;

[0039] After the reagent tube is used up, the reagent cylinder 6 is rotated by the knob 17, so that the reagent tube placed in the next placement hole 8 can be pushed out by the lifting action of the base 9, thereby realizing the continuous use of the reagent tube.

[0040] While the lid 2 pushes the L-shaped plate 12 to move through the pressure column 13, the L-shaped plate 12 drives the piston 27 to move through the connecting rod 26, so that the piston 27 draws out the air between the suction cup 23 and the table through the conduit 24, so that the suction cup 23 can be attached to the table, preventing the box 1 from moving during use and affecting the detection effect of Mycobacterium tuberculosis specific cellular immune response.

[0041] When the lid 2 is closed, the pressure rod 20 installed on the inside of the lid 2 will abut against the inclined surface of the wedge block 19, so that the pressure rod 20 pushes the cover plate 18 toward the through hole through the inclined surface of the wedge block 19, so that the cover plate 18 seals the through hole on the top plate 5, preventing the reagent tube from shaking in the reagent cylinder 6 and affecting the transfer of the reagent tube.

[0042] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0043] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A kit for detecting Mycobacterium tuberculosis-specific cellular immunoreaction, comprising a housing (1), wherein a lid (2) is hinged to the top of the housing (1), characterized in that, The box body (1) has a storage hole (3) and a placement slot (4) inside. A top plate (5) and a bottom plate (7) are fixedly installed on the inner wall of the storage hole (3). A reagent cylinder (6) is rotatably installed on the inner wall of the storage hole (3). The reagent cylinder (6) is located between the top plate (5) and the bottom plate (7). Multiple placement holes (8) are equidistantly opened in the circumferential direction at the top of the reagent cylinder (6). Corresponding through holes are opened on the upper surfaces of the bottom plate (7) and the top plate (5). The box body (1) is close to the through holes. A guide post (10) is fixedly installed on the bottom wall of the box. A conical top block (11) is fitted on the outer surface of the guide post (10). A lifting mechanism for pushing the conical top block (11) upward is provided between the box cover (2) and the box body (1). The lifting mechanism includes an L-shaped groove that is opened inside the box body (1). An L-shaped plate (12) is slidably installed on the inner wall of the L-shaped groove. One end of the L-shaped plate (12) near the guide post (10) abuts against the conical surface of the conical top block (11). The outer surface of the other end of the L-shaped plate (12) is fixedly installed with a... The pressure post (13) is abutted against the end of the pressure post (13) after the box cover (2) is fully opened. A reset assembly is installed between the L-shaped plate (12) and the bottom of the box body (1). The reset assembly includes a fixing block (14) fixedly installed on the bottom wall of the box body (1). A rectangular groove is opened through the bottom of the L-shaped plate (12). The fixing block (14) is slidably installed on the inner wall of the rectangular groove. A guide rod (15) is fixedly installed between the inner walls at both ends of the rectangular groove. A reset spring is sleeved on the outer surface of the guide rod (15). Spring (16), the reset spring (16) is set between the fixed block (14) and the inner wall of the rectangular groove away from the guide post (10). The top plate (5) has a sliding groove on the lower surface near the through hole. The inner wall of the sliding groove is slidably installed with a cover plate (18). The upper surface of the cover plate (18) is fixedly installed with two wedge blocks (19). The inner side of the box cover (2) is fixedly installed with two pressure rods (20) that are adapted to the wedge blocks (19). A tension spring (21) is fixedly installed between the cover plate (18) and the inner wall of the sliding groove.

2. The Mycobacterium tuberculosis-specific cellular immunoreaction detection kit according to claim 1, characterized in that, The bottom of the box (1) is equipped with a fixing mechanism, which includes four fixing tubes (22) that are evenly fixedly installed at the bottom of the box (1). The bottom ends of the four fixing tubes (22) are all fixedly installed with suction cups (23) that communicate with their interiors. The four fixing tubes (22) are fixedly installed with interconnected conduits (24). An air suction assembly is installed between the bottom of the box (1) and the L-shaped plate (12).

3. The Mycobacterium tuberculosis-specific cellular immunoreaction detection kit according to claim 2, characterized in that, The suction assembly includes a suction pipe (25) fixedly installed at the bottom of the box (1). The end of the suction pipe (25) near the L-shaped plate (12) is a closed structure. A piston (27) is slidably installed on the inner wall of the suction pipe (25). A connecting rod (26) is fixedly installed on the lower surface of the L-shaped plate (12). The connecting rod (26) passes through the closed end of the suction pipe (25) and is fixedly installed on the outer surface of the piston (27). The conduit (24) is connected to the inside of the closed end of the suction pipe (25).

4. The Mycobacterium tuberculosis-specific cellular immunoreaction detection kit according to claim 1, characterized in that, The inner wall of the placement hole (8) is slidably fitted with a base (9), and the bottom of the base (9) is a conical structure.

5. The Mycobacterium tuberculosis-specific cellular immunoreaction detection kit according to claim 1, characterized in that, A knob (17) is fixedly installed at the rotation center of the reagent cylinder (6).

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