Platelet-rich plasma separation device

Abstract

The application belongs to the technical field of separation devices, and particularly relates to a platelet-rich plasma separation device, which comprises a cylinder body, an upper cover, a piston and a piston push rod, the cylinder body comprises a bin body and a separation part, the bin body is connected with the upper cover at the top, and the inside is provided with the piston; the separation part is in an inverted funnel type structure, the top end of the separation part can abut against the upper cover, and the bottom end is connected with the inner wall of the cylinder body; the top end height of the separation part is slightly lower than the top end height of the side wall of the cylinder body, the separation part divides the bin body into a first bin body and a second bin body which can be communicated with each other, a channel is formed at the communication part of the first bin body and the second bin body, and the separation part and the upper cover can be matched to realize the opening or closing of the channel. The plasma in the application can be subjected to centrifugal treatment in the first bin body and the second bin body respectively, the supernatant in the second bin body is extracted by an external extraction device, and the remaining part is PRP, and the application has the advantages of convenient operation, high extraction efficiency and high extraction precision.

Description

Technical Field

[0001] This application belongs to the field of separation device technology, specifically relating to a platelet-rich plasma separation device. Background Technology

[0002] Platelet-rich plasma (PRP) is a platelet-rich product obtained by centrifuging whole blood. PRP contains a large number of growth factors and has analgesic, wound-healing, growth-promoting, and regenerative effects. High concentrations of platelets can alleviate cartilage degeneration, promote bone regeneration, and shorten wound and fracture healing time, and are widely used in orthopedic, plastic surgery, and other procedures.

[0003] Currently, due to the high liquid levels of the supernatant and platelets in the separation device after centrifugation, platelets are easily extracted during the supernatant extraction process, resulting in low extraction accuracy and low extraction efficiency.

[0004] Therefore, there is an urgent need for a platelet-rich plasma separation device with high extraction precision and efficiency. Summary of the Invention

[0005] The purpose of this application is to provide a platelet-rich plasma separation device to solve the problems of low extraction accuracy and low extraction efficiency of existing separation devices.

[0006] The embodiments of this application can be implemented through the following technical solutions:

[0007] A platelet-rich plasma separation device includes a cylinder, a top cover, a piston, and a piston push rod. The top cover is connected above the cylinder and is retractably connected to the cylinder. The piston is housed in the cylinder and is movable along the axial direction of the cylinder. The piston push rod is connected to the side of the piston away from the top cover.

[0008] The cylinder includes a chamber and a partition. The chamber is a hollow cylindrical structure, connected to the top cover at the top, and the piston is housed inside. The partition is an inverted funnel-shaped structure, with its top end abutting against the top cover and its bottom end connected to the inner wall of the cylinder.

[0009] The top of the partition is slightly lower than the top of the side wall of the cylinder. The partition divides the chamber into a first chamber and a second chamber that can communicate with each other. A channel is formed at the connection between the first chamber and the second chamber. The partition and the top cover cooperate to open or close the channel.

[0010] Furthermore, the partition includes an extension and a transition portion. The extension is a cylindrical structure that extends along the axial direction of the cylinder, and the top end of the extension can abut against the top cover. The transition portion is connected to the end of the extension away from the top cover, and the extension is connected to the inner wall of the cylinder through the transition portion.

[0011] Furthermore, the first compartment is located on the side of the partition closer to the piston.

[0012] Furthermore, the end face of the transition portion facing the upper cover has a rounded corner structure, and the partition portion is made of a smooth material.

[0013] Furthermore, the extension and the transition are connected by a rounded portion, which has a rounded corner structure.

[0014] Preferably, the rounded angle of the smooth portion is 6°, and the included angle between the transition portion and the inner wall of the second compartment is 50°.

[0015] Furthermore, the separation device employs horizontal centrifugation with a centrifugal force of less than 400g and a centrifugation time of 15 minutes.

[0016] Furthermore, the partition is made of polycarbonate material and its surface is coated with choline phosphate.

[0017] Furthermore, the upper cover has a first through hole and a second through hole, the first through hole being connected to the first compartment body and the second through hole being connected to the second compartment body.

[0018] Furthermore, the volume of the second compartment is 17ml and 23ml.

[0019] The platelet-rich plasma separation device provided in the embodiments of this application has at least the following beneficial effects:

[0020] (1) The separation device in this application divides the chamber into a first chamber and a second chamber that can communicate with each other. The connection between the first chamber and the second chamber forms a channel. The upper cover and the partition can cooperate to open or close the channel. The plasma undergoes the first centrifugation process in the first chamber, and then the upper yellow part is pushed into the second chamber for the second centrifugation process. The external extraction device removes the supernatant from the second chamber, leaving PRP. It has the advantage of convenient operation.

[0021] (2) The end face of the transition part facing the top cover in this application is a rounded structure, and the partition part is made of a smooth material. The rounded structure and the smooth material together can realize the aggregation of PRP, which can not only extract the supernatant but also prevent the lower PRP from being extracted, and also prevent PRP from aggregating into clumps, thus improving the efficiency and accuracy of PRP extraction.

[0022] (3) The separation device in this application uses horizontal centrifugation with a centrifugal force of less than 400g and a centrifugation time of 15 minutes. The platelets are stretched and deformed, which can ensure that the platelets are not activated during the preparation process to the greatest extent.

[0023] (4) The separation device in this application produces PRP through two centrifugations, and the transfer process is completed within the separation device.

[0024] The PRP production process is completely isolated from the outside world, which fundamentally reduces the possibility of contamination and is easy to operate;

[0025] (5) The red blood cell stratification in this application is convenient, which can reduce red blood cell contamination and reduce platelet loss. Therefore, the difference between different people will not be too great when operating, which can better ensure the consistency of PRB quality. Attached Figure Description

[0026] Figure 1 This is an overall structural diagram of a platelet-rich plasma separation device according to this application;

[0027] Figure 2 This is a cross-sectional view of a platelet-rich plasma separation device according to this application;

[0028] Figure 3 This is an exploded view of a platelet-rich plasma separation device according to this application;

[0029] Figure 4 This is an overall structural diagram of the piston in this application;

[0030] Figure 5 This is an overall structural diagram of the piston push rod in this application;

[0031] Figure 6 for Figure 2 A magnified view of a portion of region A in the middle.

[0032] Reference numerals: 1. Cylinder body; 11. Chamber body; 111. First chamber body; 112. Second chamber body; 12. Divider; 121. Extension; 122. Transition section; 123. Rounded section; 2. Top cover; 21. First through hole; 22. Second through hole; 3. Piston; 31. Fitting groove; 4. Piston push rod; 41. U-shaped groove; 42. Protrusion. Detailed Implementation

[0033] The present application will now be further described based on preferred embodiments and with reference to the accompanying drawings.

[0034] The vocabulary used in this specification is for illustrative purposes and is not intended to limit the scope of this application. Unless otherwise expressly specified and limited, the terms "set," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, a direct connection, or an indirect connection via an intermediate medium; or they can refer to the internal communication between two components. Those skilled in the art will understand the specific meaning of these terms in this application.

[0035] Furthermore, in the description of the embodiments of this application, various components on the drawings have been enlarged or reduced for ease of understanding, but this is not intended to limit the scope of protection of this application.

[0036] As used in this specification, the term "platelet-rich plasma (PRP)" refers to a concentrate obtained by extracting platelets from one's own blood. When blood is centrifuged, the thin intermediate layer where platelets and white blood cells aggregate is PRP, above which is a supernatant consisting of a solution without blood cell particles, and below which is a layer of red blood cells consisting of a solution containing red blood cell particles.

[0037] Figures 1-3 The overall structural diagram, cross-sectional view, and exploded view of a platelet-rich plasma separation device according to this application are shown respectively. Figures 1-3 As shown, the separation device includes a cylinder 1, an upper cover 2, and a piston 3. The upper cover 2 is connected above the cylinder 1 and can be retracted to the cylinder 1. The piston 3 is housed inside the cylinder 1 and can be displaced along the axial direction of the cylinder 1.

[0038] Furthermore, the cylinder 1 includes a compartment 11 and a partition 12. The compartment 11 is a hollow cylindrical structure, connected to the top cover 2 at the top, and houses the piston 3 inside. The partition 12 is an inverted funnel-shaped structure, with its top end abutting against the top cover 2 and its bottom end connected to the inner wall of the cylinder 1. The partition 12 divides the compartment 11 into a first compartment 111 and a second compartment 112 that can communicate with each other. The connection between the first compartment 111 and the second compartment 112 forms a channel. The top cover 2 can move away from or closer to the partition 12 along the axial direction of the cylinder 1, thereby opening or closing the channel through the cooperation of the top cover 2 and the partition 12, and thus controlling the communication or separation between the first compartment 111 and the second compartment 112.

[0039] Furthermore, the top height of the partition 12 is slightly lower than the top height of the side wall of the cylinder 1, so that the plasma in the first chamber 111 can pass over the top of the partition 12 and enter the second chamber 112.

[0040] Furthermore, the partition 12 includes an extension 121 and a transition 122. The extension 121 is a cylindrical structure and extends along the axial direction of the cylinder 1. The top end of the extension 121 can abut against the top cover 2. The transition 122 is connected to the end of the extension 121 away from the top cover 2. The extension 121 is connected to the inner wall of the cylinder 1 through the transition 122, that is, the outer side of the transition 122 is connected to the inside of the cylinder 1.

[0041] Furthermore, the first chamber 111 is located on the side of the partition 12 near the piston 3, and the extension 121 forms a narrow channel with the first chamber 111. When the piston 3 moves toward the upper cover 2, the plasma can pass through the narrow channel of the first chamber 111, the channel between the first chamber 111 and the second chamber 112, and enter the second chamber 112, thereby achieving the initial separation of plasma.

[0042] Once the plasma enters the second chamber 112, the separation device can be placed in a centrifuge for a second centrifugation. At this time, the plasma in the second chamber 112 can be separated into supernatant and PRP. The supernatant is located at the top and can be extracted and separated using an extraction device.

[0043] However, if a commercially available platelet-rich plasma (PRP) separator is used, the lower PRP layer is easily drawn away while the supernatant is being extracted due to the siphon effect of the liquid. Therefore, in this application, the end face of the transition section 122 facing the upper cover 2 is rounded, and the separator 12 is made of a smooth material. The combination of the rounded corner structure and the smooth material can achieve the aggregation of PRP, which can prevent the lower PRP layer from being drawn away and also prevent the separator 12 and the second chamber 112 from agglomerating the PRP into clumps, thereby improving the efficiency and accuracy of PRP extraction.

[0044] In some preferred embodiments of this application, the partition 12 is made of polycarbonate material and its surface is made of phosphocholine coating material.

[0045] In some preferred embodiments of this application, the cylinder 1 and the upper cover 2 are spirally connected. The outer periphery of the cylinder 1 is provided with an external thread that mates with the upper cover 2. The connection between the cylinder 1 and the upper cover 2 is achieved through the threaded connection, and the upper cover 2 can be stably displaced axially along the cylinder 1. This method is reliable and easy to process.

[0046] Therefore, when the top cover 2 is tightened downwards, the top cover 2 abuts against the top of the extension 121, and the first compartment 111 and the second compartment 112 are in a separated state; when the top cover 2 is loosened upwards, the top cover 2 separates from the top of the extension 121, and the first compartment 111 and the second compartment 112 are in a connected state.

[0047] In some preferred embodiments of this application, the separator 12 is coaxially arranged with the cylinder 1 to ensure the stability, accuracy and efficiency of plasma separation during the centrifugation process of the cylinder 1.

[0048] Furthermore, the upper cover 2 is provided with a first through hole 21 and a second through hole 22. The first through hole 21 is connected to the first compartment 111, and the second through hole 22 is connected to the second compartment 112.

[0049] Furthermore, a Luer connector is connected to the first through hole 21, which can be connected to a blood collection needle to introduce blood into the first chamber 111. The separation device is then placed in a centrifuge for a first centrifugation. After centrifugation, the plasma in the first chamber 111 becomes a red-yellow boundary, with the red portion at the bottom. The top cover 2 is then loosened upwards to separate it from the extension 121. The piston 3 is then pushed to move the plasma closer to the top cover 2. The yellow plasma enters the second chamber 112 through the narrow channel of the first chamber 111 until the red plasma is level with the top of the extension 121. At this point, the top cover 2 is tightened downwards to make it abut against the extension 121. The separation device is then placed in a centrifuge for a second centrifugation. An external extraction device is connected to the second chamber 112 through the second through hole 22. The external extraction device removes the supernatant at the top, leaving the yellow portion as the PRP to be prepared.

[0050] In some preferred embodiments of this application, the second chamber 112 is provided with graduations, so that the user can extract an appropriate volume of supernatant as needed.

[0051] Specifically, the volume of the second compartment 112 is 17ml and 23ml.

[0052] In some preferred embodiments of this application, the separation device further includes a piston push rod 4, which is connected to the side of the piston 3 away from the upper cover 2, and can drive the piston 3 to move along the axial direction of the first chamber 111. Meanwhile, when the separation device needs to be centrifuged, it is only necessary to separate the piston push rod 4 from the piston 3, and then place the separation device without the piston push rod 4 into the centrifuge.

[0053] Figure 4 , Figure 5 The overall structural diagrams of piston 3 and piston push rod 4 in this application are shown respectively, as follows: Figure 4 and Figure 5 As shown, in some preferred embodiments of this application, the rear end of the piston 3 is provided with a fitting groove 31, and the front end of the piston push rod 4 is provided with a U-shaped groove 41 to form an elastic spring portion at the front end of the piston push rod 4. The front end of the spring portion is provided with a protrusion 42 that cooperates with the fitting groove 31. When the spring portion is pressed, the front end of the piston push rod 4 deforms and can be easily disengaged from the piston 3.

[0054] In some specific embodiments of this application, such as Figure 6 As shown, the extension 121 and the transition 122 are connected by a rounded part 123, which has a rounded corner structure.

[0055] Furthermore, the angle α between the extension 121 and the transition 122, i.e. the fillet reading α of the smooth part 123, and the angle β between the transition 122 and the inner wall of the second chamber 112, will be discussed in detail below regarding their effects on platelet enrichment rate.

[0056] In some preferred embodiments of the application, the included angle β between the transition portion 122 and the inner wall of the second chamber 112 is 50°, the radius reading α of the smooth portion 123 is 6°, and after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be more than 1.8 times that of whole blood.

[0057] As a comparative embodiment 1, the included angle β between the transition section 122 and the inner wall of the second chamber 112 is 40°, the radius reading α of the smooth section 123 is 1°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.67 times that of whole blood.

[0058] In a comparative embodiment 2, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 45°, the radius reading α of the smooth section 123 is 1°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.64 times that of whole blood.

[0059] In comparative embodiment 3, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 55°, the radius reading α of the smooth section 123 is 1°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.4 times that of whole blood.

[0060] In comparative embodiment 4, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 60°, the radius reading α of the smooth section 123 is 1°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.3 times that of whole blood.

[0061] In comparative embodiment 5, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 40°, the radius reading α of the smooth section 123 is 3°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.77 times that of whole blood.

[0062] In a comparative embodiment 6, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 45°, the radius reading α of the smooth section 123 is 3°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.74 times that of whole blood.

[0063] As a comparative embodiment 7, the included angle β between the transition section 122 and the inner wall of the second chamber 112 is 55°, the radius reading α of the smooth section 123 is 3°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.5 times that of whole blood.

[0064] As a comparative embodiment 8, the included angle β between the transition section 122 and the inner wall of the second chamber 112 is 60°, the radius reading α of the smooth section 123 is 3°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.4 times that of whole blood.

[0065] As a comparative embodiment 9, the included angle β between the transition section 122 and the inner wall of the second chamber 112 is 40°, the radius reading α of the smooth section 123 is 6°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.8 times that of whole blood.

[0066] In comparative embodiment 10, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 45°, the radius reading α of the smooth section 123 is 6°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.8 times that of whole blood.

[0067] As a comparative example 11, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 55°, the radius reading α of the smooth section 123 is 6°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.6 times that of whole blood.

[0068] As a comparative example 12, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 60°, the radius reading α of the smooth section 123 is 6°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.5 times that of whole blood.

[0069] As a comparative example 13, the included angle β between the transition section 122 and the inner wall of the second chamber 112 is 40°, the rounded corner reading α of the smooth section 123 is 9°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.8 times that of whole blood.

[0070] In comparative embodiment 14, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 45°, the radius reading α of the smooth section 123 is 9°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.8 times that of whole blood.

[0071] As a comparative example 15, the included angle β between the transition section 122 and the inner wall of the second chamber 112 is 55°, the rounded corner reading α of the smooth section 123 is 9°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.6 times that of whole blood.

[0072] As a comparative example 16, the included angle β between the transition section 122 and the inner wall of the second chamber 112 is 60°, the radius reading α of the smooth section 123 is 9°, after the first centrifugation, the liquid in the second chamber 112 is shaken evenly, and the enrichment rate in the second chamber 112 is measured to be 1.5 times that of whole blood.

[0073] Furthermore, the angle β between the transition section 122 and the inner wall of the second chamber 112 is 50°, and the radius α of the smooth section 123 is 6°. After the first centrifugation, the liquid in the second chamber 112 is shaken well, and the enrichment rate in the second chamber 112 is measured to be more than 1.8 times that of whole blood. The liquid in the second chamber 112 is centrifuged a second time, the supernatant is extracted, the remaining part is shaken well, and the platelet enrichment rate is measured to be 5-6 times that of whole blood.

[0074] As a comparative example 17, the inner wall of the second chamber 112 has no coating. The liquid in the second chamber 112 is centrifuged twice, the supernatant is extracted, and the remaining part is shaken. Flocculent matter is present in the second chamber 112, and platelets aggregate to form plaques. The platelet enrichment rate is measured to be 2-3 times that of whole blood.

[0075] As a comparative example 18, the inner wall of the second chamber 112 is coated with phosphocholine. The liquid in the second chamber 112 is centrifuged twice, the supernatant is extracted, and the remaining part is shaken well. No flocculent matter is produced in the second chamber 112, and the platelet enrichment rate is measured to be 5-6 times that of whole blood.

[0076] In some preferred embodiments of this application, the separation device performs centrifugation by horizontal centrifugation with a centrifugal force of less than 400g and a centrifugation time of 15 minutes. Under these centrifugation conditions, platelets are subjected to less stretching deformation, which can maximize the protection of platelets from activation during the preparation process, thereby further ensuring the efficiency and enrichment rate of PRP preparation.

[0077] The specific embodiments of this application have been described in detail above. For those skilled in the art, several improvements and modifications can be made to this application without departing from the principle of this application, and these improvements and modifications also fall within the protection scope of the claims of this application.

Claims

1. A platelet-rich plasma separation device, comprising a cylinder (1), a top cover (2), a piston (3), and a piston push rod (4), wherein the top cover (2) is connected above the cylinder (1) and retractably connected to the cylinder (1), the piston (3) is housed within the cylinder (1) and is movable along the axial direction of the cylinder (1), and the piston push rod (4) is connected to the side of the piston (3) away from the top cover (2), characterized in that: The cylindrical body (1) includes a compartment (11) and a partition (12). The compartment (11) is a hollow cylindrical structure, which is connected to the top cover (2) at the top and contains the piston (3). The partition (12) is an inverted funnel-shaped structure, whose top end can abut against the top cover (2) and whose bottom end is connected to the inner wall of the cylindrical body (1). The top of the partition (12) is slightly lower than the top of the side wall of the cylinder (1). The partition (12) divides the compartment (11) into a first compartment (111) and a second compartment (112) that can communicate with each other. A channel is formed at the connection between the first compartment (111) and the second compartment (112). The partition (12) can be opened or closed by cooperating with the top cover (2). The partition (12) includes an extension (121) and a transition (122). The extension (121) is a cylindrical structure and extends along the axial direction of the cylinder (1). The top of the extension (121) can be connected to the top cover. (2) Abutting; the transition part (122) is connected to the end of the extension part (121) away from the top cover (2), and the extension part (121) is connected to the inner wall of the cylinder (1) through the transition part (122); the end face of the transition part (122) facing the top cover (2) is a rounded corner structure, and the partition part (12) is made of a smooth material; the extension part (121) and the transition part (122) are connected through a rounded part (123), and the rounded part (123) is a rounded corner structure; the rounded angle of the rounded part (123) is 6°, and the included angle between the transition part (122) and the inner wall of the second compartment (112) is 50°.

2. The platelet-rich plasma separation device according to claim 1, characterized in that: The first compartment (111) is located on the side of the partition (12) near the piston (3).

3. The platelet-rich plasma separation device according to claim 1, characterized in that: The separation device uses horizontal centrifugation with a centrifugal force of less than 400g and a centrifugation time of 15 minutes.

4. The platelet-rich plasma separation device according to claim 1, characterized in that: The partition (12) is made of polycarbonate material and has a choline phosphate coating on its surface.

5. The platelet-rich plasma separation device according to claim 1, characterized in that: The upper cover (2) has a first through hole (21) and a second through hole (22). The first through hole (21) is connected to the first compartment (111), and the second through hole (22) is connected to the second compartment (112).

6. The platelet-rich plasma separation device according to claim 1, characterized in that: The volume of the second compartment (112) is 17ml and 23ml.

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