一种靶向敲除RIPK1基因的Neuro-2a细胞模型及其应用

By targeting and knocking out the RIPK1 gene in Neuro-2a cells using CRISPR/Cas9 technology, a cell model of RIPK1 gene loss of function was constructed. This fills the research gap on the impact of RIPK1 gene deletion on Neuro-2a cell function and the infection mechanism of Japanese encephalitis virus, and provides important support for drug screening.

CN118222574BActive Publication Date: 2026-07-17HENAN AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HENAN AGRICULTURAL UNIVERSITY
Filing Date
2024-04-25
Publication Date
2026-07-17

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Abstract

本发明公开了一种靶向敲除RIPK1基因的Neuro‑2a细胞模型及其应用，属于分子生物学与细胞生物学领域，本发明公开了一种靶向敲除RIPK1基因的sgRNA，包含sgRNA‑1、sgRNA‑2或sgRNA‑3。本发明还公开了一种Neuro‑2a细胞模型，所述Neuro‑2a细胞模型为RIPK1基因敲除的Neuro‑2a细胞系。本发明制备了敲除RIPK1基因的Neuro‑2a细胞系，该敲除细胞与野生型细胞相比，生长功能不受影响，为研究RIPK1基因在乙型脑炎病毒复制方面的功能提供有效的细胞模型，并为RIPK1基因缺失与乙型脑炎病毒感染Neuro‑2a细胞的分子机制相关研究奠定重要基础。
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