一种铁纳米酶及其制备方法和快速检测诺氟沙星中的应用

By preparing dumbbell-shaped iron nanozymes and combining them with enzyme inhibitors and chromogenic substrates, a rapid and low-cost quantitative detection of norfloxacin was achieved, solving the problems of high detection cost and poor stability in existing technologies, and providing a highly sensitive and selective method for the detection of norfloxacin.

CN118268037BActive Publication Date: 2026-07-17NANCHANG UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANCHANG UNIV
Filing Date
2024-03-21
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing norfloxacin detection methods are costly, have poor operational stability, are sensitive to reaction environments, are difficult to recover and reuse, and are time-consuming and complex, which limits their application in rapid detection.

Method used

Dumbbell-shaped iron nanozymes were prepared, and norfloxacin was visualized and detected using an ultraviolet spectrophotometer or RGB extraction detection method with enzyme inhibitors and chromogenic substrates. This simplified the pretreatment steps, reduced costs, and improved sensitivity and accuracy.

Benefits of technology

It enables rapid, simple, and low-cost quantitative detection of norfloxacin, with a detection range of 0.05-6.00 μmol/L and a detection limit of 27 nmol/L. It has good selectivity and anti-interference ability and is suitable for on-site visual detection.

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Abstract

本发明涉及污染物检测技术领域,尤其涉及一种铁纳米酶及其制备方法和快速检测诺氟沙星中的应用。该方法包括以下步骤:将待测水样品与酶抑制物、缓冲液、过氧化物、显色底物、哑铃状铁纳米酶混和后得到反应液一,使用紫外分光光度计测定所述反应液一的吸光度,从而得到所述待测样品中的诺氟沙星含量;或通过手机软件提取反应液一图片中的RGB值,从而得到所述待测样品中的诺氟沙星含量。本发明提供的方法利用纳米酶的类酶活性与比色化学传感技术结合,具有成本低、操作简单便利、高灵敏度和准确性等特点,能够实现现场可视化快速检测样品中诺氟沙星含量,具有重要的实际意义。
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