Method and apparatus for preparing microbial culture medium liquid from organic anaerobic fermentation liquid

Abstract

The application discloses a method and equipment for preparing microbial culture medium liquid from organic anaerobic fermentation liquid. The method comprises the following steps: adding and mixing a controlled amount of medicine in the organic anaerobic fermentation liquid, rapidly intercepting and processing the formed suspended organic matter polymer through matched MBR equipment, retaining other water-soluble nitrogen, phosphorus, potassium and other organic nutrients, introducing the purified water into an ozone reaction tower to simultaneously perform deodorization, sterilization, nitrification and decolorization treatment, and finally obtaining the culture medium liquid for microbial culture. The application combines the existing process, adopts a non-biological treatment process according to the characteristics of the organic anaerobic fermentation liquid, reasonably selects the treatment strength of each process link according to different sewage properties and different nutritional requirements of microorganisms, custom-produces the culture medium liquid meeting the requirements, and simultaneously innovates the existing process and equipment, so that the efficient, stable and low-cost treatment mode is realized.

Description

Technical Field

[0001] This invention relates to the field of biogas slurry treatment technology, specifically to a method and equipment for preparing microbial culture medium from organic anaerobic fermentation broth. Background Technology

[0002] Existing microbial culture media, such as those containing bacteria and algae, are mainly composed of various nutrients, proteins, and carbon sources. In actual large-scale production, large quantities of expensive fertilizers such as urea and potassium dihydrogen phosphate are required, making production costs a bottleneck restricting the development of the microbial industry. In organic anaerobic fermentation broth, macromolecules are decomposed into easily absorbed water-soluble and readily available nutrients such as nitrogen, phosphorus, and potassium, as well as small molecules with specific physiological functions, such as amino acids, plant hormones, vitamins, and NH4+. 4 With its high nutrient availability and strong ability to provide rapid nutrition (including ions, organic matter, humic acid, etc.), organic anaerobic fermentation broth can be quickly absorbed and utilized by microorganisms. Therefore, after specific treatment, organic anaerobic fermentation broth can be used as a base liquid for microbial production, thereby replacing chemical fertilizers and reducing the cost of microbial production in large quantities, which is a feasible direction.

[0003] Furthermore, the total amount of nutrients such as nitrogen cannot be used as the sole criterion for microbial culture media. Studies have shown that microorganisms have varying needs for NO3-. - -N, NH4 + -N, NO2 - The requirements for different nitrogen forms, such as -N, also vary greatly. Different forms and concentrations of nitrogen will have different effects on the growth of microorganisms, and may even directly affect the synthesis of certain proteins, polysaccharides, amino acids, vitamins, etc. Therefore, how to achieve the controllability of nitrogen form and ratio is also a key technology that must be possessed in the preparation of excellent microbial culture media.

[0004] Traditional organic anaerobic fermentation broth resource utilization mainly relies on returning it to the field for disposal. The overall technical approach emphasizes harmlessness and does not give much consideration to the nutritional needs of crops. Treatment processes commonly employ biotechnology such as A / O, SBR, and ANAMMOX. Water-soluble, readily available organic nutrients such as nitrogen, phosphorus, and potassium are simultaneously reduced during the treatment of other limited indicators, and this reduction cannot be quantitatively controlled. Therefore, it fails to achieve the goals of wastewater purification, efficient nutrient recovery, and the production of high-value biomass, and the value of waste resources is not effectively transferred. Furthermore, these biological treatment systems are susceptible to environmental influences, have poor stability, weak resistance to water quality fluctuations, require high control over the treatment process, have low treatment efficiency, require relatively large land areas, have complex processes, and long treatment times.

[0005] Currently, there is no method for preparing microbial culture medium from organic anaerobic fermentation broth that can maximize the retention of water-soluble beneficial nutrients in the broth while controlling multiple beneficial microbial production conditions such as total nutrient content, nitrogen form, and quality safety. Summary of the Invention

[0006] To address the aforementioned technical problems, this invention provides a method and apparatus for preparing microbial culture medium from organic anaerobic fermentation broth.

[0007] The technical solution of the present invention is: a method for preparing microbial culture medium from organic anaerobic fermentation broth. The method involves adding and mixing the organic anaerobic fermentation broth with controlled amounts of chemicals to form suspended organic matter aggregates, which are then rapidly intercepted and processed by a matching MBR device. Water-soluble organic nutrients in the organic anaerobic fermentation broth are retained. The purified water is introduced into an ozone reaction tower for simultaneous deodorization, sterilization, nitrification, and decolorization, ultimately yielding a culture medium for microbial cultivation. The COD of the organic anaerobic fermentation broth is 3000mg / L-7000mg / L; the amount of the controlled-dosing agent added per ton of organic anaerobic fermentation broth is 0.5kg-5kg, and the dosage of the agent is controlled according to the filtration accuracy of the MBR equipment and the nutritional indicators of the microbial culture medium. The organic nutrients are water-soluble organic nutrients such as nitrogen, phosphorus, and potassium.

[0008] Furthermore, the concentrated water produced by the MBR equipment is introduced into the sludge tank, and the concentrated water is filtered by a filter press to obtain tailwater and sludge. The tailwater is then combined with organic anaerobic fermentation liquid for recycling. At the same time, the sludge is treated to obtain organic fertilizer.

[0009] Explanation: By setting up sludge bins and filter presses, effluent and sludge can be separated, allowing for subsequent resource recovery of the sludge and recycling of the effluent, thus ensuring the harmless output of the entire process.

[0010] Furthermore, the method includes the following steps: Step 1: Pass the organic anaerobic fermentation broth into the dosing tube, and add the dosing agent in a controlled amount to carry out the mixing reaction and form a suspended organic matter polymer. Step 2: The organic anaerobic fermentation broth treated in Step 1 is introduced into the MBR equipment. Suspended organic matter polymers that meet the filtration precision of the MBR equipment are quickly intercepted. While significantly reducing the COD, SS, turbidity, and color of the fermentation broth, water-soluble organic nutrients such as nitrogen, phosphorus, and potassium in the fermentation broth are retained. The purified water and concentrated water are obtained through rapid filtration treatment by the MBR equipment. The COD of the purified water should be controlled between 400 mg / L and 600 mg / L, depending on the nutritional requirements of different microorganisms. Step 3: The concentrated water obtained in Step 2 is introduced into the sludge tank. The concentrated water is filtered by a filter press to obtain tailwater and sludge. The tailwater is introduced into the dosing pipe to merge with the organic anaerobic fermentation liquid. The sludge is then treated to obtain organic fertilizer. Step 4: The purified water obtained in Step 2 is introduced into the ozone reaction tower for simultaneous deodorization, sterilization, nitrification and decolorization treatment. The ozone reaction intensity is controlled and the nitrogen and phosphorus elements are reasonably adjusted to finally obtain the culture medium solution for microbial culture.

[0011] Description: The method of this invention uses controlled dosage and MBR filtration to treat organic anaerobic fermentation broth in a targeted manner, which can retain a large amount of water-soluble organic nutrients such as nitrogen, phosphorus, and potassium in the organic anaerobic fermentation broth, thereby processing the organic anaerobic fermentation broth into a culture medium for microbial culture and realizing the resource utilization of organic anaerobic fermentation broth.

[0012] Furthermore, the agent added in the controlled dosage is polyaluminum chloride (PAC) flocculant; the ozone injection rate of the ozone reaction tower is 50~200g / h.

[0013] Note: The amount of polyaluminum chloride (PAC) flocculant added is easy to control. It can flocculate small suspended particles in organic anaerobic fermentation broth into large particles, which facilitates subsequent membrane filtration and separation in MBR equipment.

[0014] This invention also provides an apparatus for preparing microbial culture medium from organic anaerobic fermentation broth. Based on the above-mentioned method for preparing microbial culture medium from organic anaerobic fermentation broth, the apparatus includes a box, and a mixing chamber for mixing the agent and the aquaculture biogas slurry, a sludge chamber for collecting concentrated water, an ozone treatment chamber for treating the purified water with ozone, an MBR device for filtering and removing impurities from the aquaculture biogas slurry, and a dosing pipe for controlling the dosage of the agent in the aquaculture biogas slurry. The MBR equipment is mounted above the sludge tank. An inlet for connecting to the dosing pipe is located on one side of the mixing tank. A controlled-release device is installed on the dosing pipe. An outlet connected to the vertical pipe of the MBR equipment via an inclined pipe is located on the partition on the other side of the mixing tank. The ozone treatment chamber is equipped with an ozone reaction tower. The inlet of the ozone reaction tower is connected to the purified water outlet of the MBR equipment via a pipeline. The concentrated water outlet at the bottom of the MBR equipment corresponds to the position of the sludge tank. The sludge silo is equipped with a filter press, and the sludge silo is also equipped with a tailwater outlet that is connected to a dosing pipe via a pipeline.

[0015] Note: The above equipment can meet the requirements of the organic anaerobic fermentation broth preparation microbial culture medium liquid process of the present invention, and has the advantages of high efficiency, stability and low cost. At the same time, it can also treat sludge for resource utilization and realize the harmless output of the entire treatment system.

[0016] Furthermore, the mixing chamber is equipped with multiple vertically staggered baffles between the inlet and outlet to form an S-shaped flow path, and multiple agitators are installed inside the mixing chamber.

[0017] Explanation: Designing the mixing chamber with an S-shaped flow path can significantly increase the residence time of the organic anaerobic fermentation broth in the mixing chamber, thereby allowing the reagents to be fully mixed with the organic anaerobic fermentation broth by the mixer.

[0018] Furthermore, the MBR equipment includes a housing, and a plurality of tubular membranes and a suction pump assembly disposed within the housing; The shell consists of a cylindrical part at the top and a conical part at the bottom. The upper part of the cylindrical part is provided with a first flow control tube assembly for connecting the upper end of the tubular membrane and entering the outlet to discharge the organic anaerobic fermentation liquid. The lower part of the cylindrical part is provided with a second flow control tube assembly for connecting the lower end of the tubular membrane and controlling the concentrated water to fall into the conical part. The first flow control tube assembly is connected to the liquid inlet provided on the shell. The bottom of the conical part is provided with a concentrated water outlet. The suction pump assembly includes a multi-port suction pipe sleeved on several tubular membranes, and a suction pump whose inlet end is connected to the multi-port suction pipe. The outlet end of the suction pump is connected to the clean water outlet provided on the housing through a flexible hose with a length margin. Each port of the multi-port suction pipe has an opening and the upper and lower ends of the port are rounded. The inner wall of the housing is provided with a slide rail that is slidably connected to the multi-port suction pipe. The cylindrical part located on the side wall of the inlet is provided with a drive assembly for reciprocatingly driving the suction pump assembly to move up and down using the flow of organic anaerobic fermentation liquid. The drive assembly includes a crank rod rotatably mounted on the inner wall of the cylindrical part, a water wheel mounted in the pipe and fixedly connected at one end to the center end of the crank rod via a shaft, and a carrier plate slidably mounted on the inner wall of the cylindrical part. One end of the carrier plate is provided with a guide rail perpendicular to it. The guide rail is slidably connected to the eccentric end of the crank rod via a slider. The carrier plate is detachably connected to the multi-port suction pipe. A control gear is rotatably mounted on the side wall of the cylindrical part located on the side of the liquid inlet. The carrier plate has a toothed segment that meshes with the control gear. One end of the second flow control tube assembly is rotatably mounted with a control rod for rotating and controlling the opening of the second flow control tube assembly. The control rod is provided with a groove, and the control gear is provided with a paddle block for cooperating with the groove to rotate the control rod. A water baffle is provided inside the housing located below the purified water outlet, and the outlet of the second flow control tube assembly passes through the water baffle and connects to the concentrated water outlet. The tubular membrane is equipped with a magnetic ring that can move up and down. The magnetic ring is connected to the first flow control tube assembly through a rod assembly. The multi-port suction tube is equipped with a magnetic block that interacts with the magnetic ring. The rod assembly consists of a rod body and several protrusions on the rod body. The magnetic ring is slidably sleeved on the rod body. The magnetic ring is equipped with a torsion spring striking block that cooperates with the protrusions. The sidewall of the magnetic ring has several bristles.

[0019] Explanation: The above-mentioned structural design of the MBR equipment can make full use of the water flow potential energy generated when the organic anaerobic fermentation broth is pumped to the MBR equipment. This water flow potential energy is used to drive the components, so that the multi-port suction tube can move up and down reciprocatingly. In addition, the elastic properties of the tubular membrane can effectively improve the efficiency of MBR membrane filtration. Meanwhile, with the magnetic ring in place, the up-and-down reciprocating motion of the multi-port suction tube, combined with the telescopic component, causes the magnetic ring to vibrate, and the brush bristles accelerate the fall of concentrated water. Furthermore, under the driving action of the drive component, the second flow control tube assembly can be periodically opened in conjunction with the control rod and control gear, thereby saving the number of electric drive components, reducing equipment energy consumption, and saving energy and protecting the environment.

[0020] Furthermore, the other end of the water wheel is sealed through the pipe via a rotating shaft, and a motor connected to the shaft via an output shaft is provided on the pipe; the multi-port suction pipe is provided with a flushing pipe for backwashing the tubular membrane, and the flushing pipe is connected to the water pump of the external water tank via a flexible hose with a length allowance.

[0021] Explanation: By starting the motor during backwashing, the multi-port suction pipe can move up and down reciprocally, thereby causing the flushing pipe to flush up and down repeatedly. Compared to backwashing the tubular membrane in full coverage, the required water pressure is greatly reduced, thus reducing the energy consumption of the water pump. Moreover, this up-and-down reciprocating flushing method can improve the efficiency of backwashing, thereby avoiding the impact of incomplete backwashing on the efficiency of reuse.

[0022] Furthermore, the outlet of the ozone reaction tower is equipped with a filter tube filled with activated carbon.

[0023] Note: By installing a filter tube filled with activated carbon at the outlet of the ozone reaction tower, the culture medium solution can be filtered twice, thereby obtaining a high-quality culture medium solution for microbial culture.

[0024] Furthermore, the box is covered with a cover, and the bottom of the box is equipped with casters.

[0025] Note: By placing the device in a movable housing, it can be easily moved as a whole and used in multiple scenarios. At the same time, the core components of the device can be covered to prevent damage caused by dust, collisions, etc., thereby improving the service life of the device.

[0026] The beneficial effects of this invention are: (1) The method of the present invention uses a combination of controlled dosage and MBR filtration to treat organic anaerobic fermentation broth in a targeted manner, which can retain a large amount of water-soluble organic nutrients such as nitrogen, phosphorus and potassium in the organic anaerobic fermentation broth, thereby processing the organic anaerobic fermentation broth into a culture medium for microbial culture, realizing the high-value resource utilization of organic anaerobic fermentation broth.

[0027] (2) The method and equipment of this invention solve the problems of the original organic anaerobic fermentation liquid treatment system, which occupies a large area and mostly uses A / O or A 2 The / O process is complex, difficult to operate, and the microorganisms are greatly affected by the environment, making them unstable. It also has a long reaction time, high cost, and poor results.

[0028] (3) At present, there is no conventional treatment method for organic anaerobic fermentation liquids such as livestock and poultry breeding, food production, and kitchen waste that does not require biological process treatment. Although there are membrane treatment methods on the market, traditional membrane filtration is expensive. Therefore, this invention combines existing processes, takes into full account the characteristics of organic anaerobic fermentation liquids, and fully considers factors such as treatment cost and efficiency. It adopts a gradient treatment process and innovates on existing processes and equipment to achieve a high-efficiency, stable and low-cost treatment mode.

[0029] (4) The equipment of the present invention can meet the requirements of the organic anaerobic fermentation liquid preparation microbial culture medium liquid process of the present invention, and has the advantages of high efficiency, stability and low cost. At the same time, it can also treat sludge for resource utilization and realize the harmless output of the entire treatment system. Attached Figure Description

[0030] Figure 1 This is a flowchart of the method of the present invention.

[0031] Figure 2 This is a schematic diagram of the overall structure of the device of the present invention. Figure 1 ; Figure 3 This is a schematic diagram of the overall structure of the device of the present invention. Figure 2 ; Figure 4 This is a schematic diagram of the internal structure of the device of the present invention; Figure 5 This is a schematic diagram of the structure of the MBR device of the present invention; Figure 6 This is a schematic diagram of the internal structure of the MBR device of the present invention; Figure 7 This is a schematic diagram of the assembly structure of the multi-port suction tube and tubular membrane of the present invention; Figure 8 This is a schematic diagram of the assembly structure of the drive component and the second flow control tube assembly of the present invention; Figure 9 This is a schematic diagram of the assembly structure of the carrier plate and crankshaft of the drive component of the present invention; Figure 10 This is a schematic diagram of the assembly structure of the control gear and control rod of the drive component of the present invention; Figure 11 This is a schematic diagram of the assembly structure of the second flow control tube assembly and the control rod of the present invention; Figure 12 This is a schematic diagram of the structure of the multi-port suction tube of the present invention; Figure 13 This is a schematic diagram of the assembly structure of the magnetic ring and the rod body of the present invention; Among them, 1-box body, 2-mixing chamber, 21-baffle, 3-sludge chamber, 4-ozone treatment chamber, 5-MBR equipment, 51-shell, 511-clean water outlet, 512-concentrate water outlet, 52-tubular membrane, 53-first flow control pipe assembly, 54-second flow control pipe assembly, 55-multi-port suction pipe, 551-suction pump, 56-flushing pipe, 57-vertical pipe, 58-inclined pipe, 6-dosing pipe, 7-drive assembly, 71-crank rod, 711-slider, 72-water wheel, 73-carrier plate, 731-tooth segment, 74-guide rail, 75-rotation control gear, 76-rotation control rod, 77-groove, 78-rotation block, 8-magnetic ring, 81-magnetic block, 82-rod body, 83-protrusion, 9-cover, 10-filter tube.

[0032] Figure 14 The diagrams are colorimetric changes in water treatment in Application Example 1 of the present invention, wherein (a) is the colorimetric diagram of organic anaerobic fermentation broth A, (b) is the colorimetric diagram of pretreated broth B, and (c) is the colorimetric diagram of culture medium broth C.

[0033] Figure 15 This is an application diagram of Rhodopseudomonas palustris (photosynthetic bacteria) in the production of this invention, as shown in Application Example 1.

[0034] Figure 16 The diagrams are colorimetric changes in water treatment in Example 2 of this invention. (a) is the colorimetric diagram of organic anaerobic fermentation broth A, (b) is the colorimetric diagram of pretreated broth B, and (c) is the colorimetric diagram of culture medium C.

[0035] Figure 17 This is a production diagram of Chlorella in Application Example 2 of the present invention. Detailed Implementation

[0036] The present invention will now be described in more detail with reference to specific embodiments, so as to better demonstrate the advantages of the present invention.

[0037] Example 1: A method for preparing microbial culture medium from organic anaerobic fermentation broth. The method involves adding chemicals to the organic anaerobic fermentation broth in controlled quantities to form suspended organic matter aggregates, which are then rapidly intercepted and treated by a matching MBR device 5. Water-soluble organic nutrients in the organic anaerobic fermentation broth are retained. The purified water is introduced into an ozone reaction tower for simultaneous deodorization, sterilization, nitrification, and decolorization, ultimately yielding a culture medium for microbial cultivation. The concentrated water produced by the MBR equipment 5 is introduced into the sludge tank 3. The concentrated water is filtered by a filter press to obtain tailwater and sludge. The tailwater is then combined with organic anaerobic fermentation liquid for recycling. At the same time, the sludge is treated to obtain organic fertilizer. The COD of the organic anaerobic fermentation broth is 4000 mg / L; the amount of the controlled-dosing agent added per ton of organic anaerobic fermentation broth is 0.7 kg, and the controlled-dosing agent is commercially available polyaluminum chloride flocculant; the ozone flow rate of the ozone reaction tower is 60 g / h; the organic nutrients are nitrogen, phosphorus, and potassium. It is understood that the organic nutrients are not limited to these three water-soluble organic nutrients. For the convenience of describing the scheme, nitrogen, phosphorus, and potassium are used as the contents of water-soluble organic nutrients in this description. like Figure 1 As shown, the above method specifically includes the following steps: Step 1: Pass the organic anaerobic fermentation broth into the dosing tube 6, and add the dosing agent in a controlled manner through the dosing tube 6 to carry out the mixing reaction and form a suspended organic matter polymer. Step 2: The organic anaerobic fermentation broth treated in Step 1 is introduced into MBR device 5. Suspended organic matter polymers that meet the filtration precision of MBR device 5 are quickly intercepted. While significantly reducing COD, SS, turbidity and color of fermentation broth, water-soluble nitrogen, phosphorus and potassium organic nutrients in fermentation broth are retained. The purified water and concentrated water are obtained through rapid treatment by MBR device 5. Understandably, the membranes used in MBR equipment 5 are made of materials such as PAN, PSF, PVC, and PVDF. Depending on the water quality, the filtration accuracy should be controlled between 0.001μm and 0.03μm. Step 3: The concentrated water obtained in Step 2 is introduced into the sludge tank 3. The concentrated water is filtered by a filter press to obtain tailwater and sludge. The tailwater is introduced into the dosing pipe 6 to merge with the organic anaerobic fermentation liquid. The sludge is then treated to obtain organic fertilizer. Step 4: The purified water obtained in Step 2 is introduced into the ozone reaction tower for simultaneous deodorization, sterilization, nitrification and decolorization treatment, and finally a culture medium solution for microbial culture is obtained.

[0038] Example 2: This example differs from Example 1 in that the COD of the organic anaerobic fermentation liquid is 3000 mg / L; the amount of the controlled dosage of the added agent is 0.5 kg per ton of organic anaerobic fermentation liquid; and the ozone flow rate of the ozone reaction tower is 50 g / h.

[0039] Example 3: This example differs from Example 1 in that the COD of the organic anaerobic fermentation liquid is 7000 mg / L; the amount of the controlled dosage of the added agent is 5 kg per ton of organic anaerobic fermentation liquid; and the ozone flow rate of the ozone reaction tower is 200 g / h.

[0040] Example 4: The present invention also provides an apparatus for preparing microbial culture medium from organic anaerobic fermentation broth, based on the above-described method for preparing microbial culture medium from organic anaerobic fermentation broth, such as... Figures 2-4 As shown, the equipment includes a housing 1, and a mixing chamber 2 for mixing the agent and the aquaculture biogas slurry, a sludge chamber 3 for collecting concentrated water, an ozone treatment chamber 4 for treating the purified water with ozone, an MBR device 5 for filtering and removing impurities from the organic anaerobic fermentation liquid, and a dosing pipe 6 for controlling the dosage of the agent in the aquaculture biogas slurry. Multiple baffles 21 are vertically arranged in a staggered pattern within the mixing chamber 2 between the inlet and outlet, forming an S-shaped flow path. The mixing chamber 2 contains three mixers; these mixers refer to commercially available mixers, and each mixer is located in one of the three compartments. The MBR equipment 5 is mounted above the sludge tank 3. The mixing tank 2 has an inlet on one side of its housing 1 for connection to the dosing pipe 6. The dosing pipe 6 is equipped with a controlled-release device. The other side of the mixing tank 2 has an outlet on its partition that connects to the vertical pipe 57 of the MBR equipment 5 via an inclined pipe 58. The ozone treatment tank 4 contains an ozone reaction tower. It is understood that the ozone reaction tower is a commercially available ozone reaction tower. The inlet of the ozone reaction tower is connected to the purified water outlet of the MBR equipment 5 via a pipe. The concentrated water outlet at the bottom of the MBR equipment 5 corresponds to the position of the sludge tank 3. The sludge tank 3 contains a filter press, and the sludge tank 3 has a tailwater outlet connected to the dosing pipe 6 via a pipe.

[0041] Example 5: This example differs from Example 4 in that, as Figures 5-12As shown, the MBR equipment 5 includes a housing 51, and a plurality of tubular membranes 52 and a suction pump assembly disposed within the housing 51; the housing 51 is composed of a cylindrical part at the top and a conical part at the bottom. The upper part of the cylindrical part is provided with a first flow control pipe assembly 53 for connecting the upper end of the tubular membranes 52 and connecting to the outlet of the organic anaerobic fermentation liquid, and the lower part is provided with a second flow control pipe assembly 54 for connecting the lower end of the tubular membranes 52 and controlling the concentrated water to fall into the conical part. The first flow control pipe assembly 53 is connected to the inlet provided on the housing 51, and the bottom of the conical part is provided with a concentrated water outlet 512; It is understandable that both the first flow control assembly 53 and the second flow control assembly 54 are as follows: Figure 7 The assembly shown consists of two arc-shaped plates and nine tubes positioned between them. The suction pump assembly includes a multi-port suction pipe 55 sleeved on several tubular membranes 52, and a suction pump 551 whose inlet end is connected to the multi-port suction pipe 55. The outlet end of the suction pump 551 is connected to the clean water outlet 511 provided on the housing 51 through a flexible hose with a length allowance. Each port of the multi-port suction pipe 55 has an opening and the upper and lower ends of the port are rounded. The inner wall of the housing 51 is provided with a slide rail that is slidably connected to the multi-port suction pipe 55. like Figure 8 , Figure 9 As shown, a drive assembly 7 is provided on the side wall of the cylindrical part located on the side of the liquid inlet. This drive assembly 7 is used to drive the suction pump assembly to move up and down by reciprocating the flow of organic anaerobic fermentation liquid. The drive assembly 7 includes a crank rod 71 rotatably mounted on the inner wall of the cylindrical part, a water wheel 72 mounted in the pipe and fixedly connected at one end to the center end of the crank rod 71 by a shaft, and a carrier plate 73 slidably mounted on the inner wall of the cylindrical part. One end of the carrier plate 73 is provided with a guide rail 74 perpendicular to it. The guide rail 74 is slidably connected to the eccentric end of the crank rod 71 by a slider 711. The carrier plate 73 is detachably connected to the multi-port suction pipe 55. like Figure 10 , Figure 11 As shown, a control gear 75 is rotatably mounted on the side wall of the cylindrical portion located on the side of the inlet. A toothed segment 731 meshes with the control gear 75 on the carrier plate 73. A control rod 76 is rotatably mounted at one end of the second flow control tube assembly 54 for rotating and controlling the opening of the second flow control tube assembly 54. The control rod 76 has a groove 77, and the control gear 75 has a lever 78 for engaging the groove 77 to rotate the control rod 76. A water-blocking plate is located inside the housing 51 below the purified water outlet 511, and the outlet of the second flow control tube assembly 54 passes through the water-blocking plate and connects to the concentrated water outlet 512. It is understandable that the rotating sealing sleeve inside the second flow control tube of the second flow control tube assembly is provided with, for example... Figure 11The control rod 76 shown is a hollow tube that can connect the upper and lower pipe ports by rotating 60°. like Figure 13 As shown, the tubular membrane 52 contains a magnetic ring 8 that can move up and down. The magnetic ring 8 is connected to the first flow control tube assembly 53 via a rod assembly. The multi-port suction tube 55 is provided with a magnetic block 81 that interacts with the magnetic ring 8. The rod assembly consists of a rod body 82 and several protrusions 83 on the rod body 82. The magnetic ring 8 is slidably sleeved on the rod body 82, and the magnetic ring 8 is provided with a torsion spring striking block that cooperates with the protrusions 83. The sidewall of the magnetic ring 8 has several bristles. like Figure 12 As shown, the other end of the water wheel 72 is sealed through the pipe by rotating the shaft, and the pipe is equipped with a motor connected to the shaft through the output shaft; the multi-port suction pipe 55 is equipped with a flushing pipe 56 for backwashing the tubular membrane 52, and the flushing pipe 56 is connected to the water pump of the external water tank through a flexible hose with a length margin.

[0042] The working principle of the above-mentioned MBR equipment 5 is as follows: The organic anaerobic fermentation liquid after step 1 is introduced into the pipeline of the MBR equipment 5. The organic anaerobic fermentation liquid drives the water wheel 72 to rotate. The rotation of the water wheel 72 causes the carrier plate 73 to move up and down reciprocally. The carrier plate 73 drives the suction pump assembly to move up and down reciprocally, thereby suctioning the purified water. At the same time as suction, the magnetic block 81 of the suction pump assembly will drive the magnetic ring 8 to move up and down inside the tubular membrane 52. With the cooperation of the telescopic component, the magnetic ring 8 vibrates. The brush bristles scrape the deposits inside the tubular membrane 52, thereby improving the suction efficiency. Meanwhile, as the carrier plate 73 moves downward, the control gear 75 is rotated by the tooth segment 731, so that the paddle block 78 provided on the control gear 75 cooperates with the paddle groove 77 to rotate the control rod 76, thereby opening the second flow control tube assembly 54, so that the concentrate in the tubular membrane 52 falls into the conical part and flows into the sludge bin 3 through the concentrate outlet 512. When backwashing is performed, the motor is started. In the same way as above, the up-and-down reciprocating motion of the suction pump assembly drives the flushing pipe to flush the tubular membrane 52, and works in conjunction with the magnetic ring 8. At the same time, negative pressure can be applied to the pipeline to improve the backwashing effect.

[0043] Example 6: This example differs from Example 5 in that, as... Figure 4 As shown, the outlet of the ozone reaction tower is equipped with a filter tube 10 filled with activated carbon. It can be understood that the activated carbon is commercially available activated carbon with a particle size of 0.5mm-0.8mm.

[0044] Example 7: This example differs from Example 5 in that, as Figure 2As shown, the box body 1 is covered with a cover 9, and the bottom surface of the box body 1 is provided with six sets of universal wheels, including four sets of universal wheels located at the four corners of the bottom surface of the box body 1 and two sets of universal wheels located at the center of the bottom surface of the box body 1. It can be understood that the universal wheels are selected from commercially available products.

[0045] Application Example 1: The indicators of the bottom liquid from the brewing cellar of a brewing enterprise in Guizhou are shown in Table 1. After being treated by the factory's existing two-stage high-efficiency anaerobic facilities, the bottom liquid was used to obtain organic anaerobic fermentation liquid A, the indicators of which are shown in Table 2. Table 1. Indicators of the bottom liquid in brewing cellars

[0046] Note: Other toxic and hazardous substance indicators all meet the limit standards, which are omitted here.

[0047] Table 2. Organic Anaerobic Fermentation Broth A Index

[0048] The above-mentioned organic anaerobic fermentation broth A was treated using the method of Example 1 and the equipment of Example 4. The organic anaerobic fermentation broth A was fed into the equipment, and 0.7 kg of polyaluminum chloride flocculant was added per ton of organic anaerobic fermentation broth A. After thorough mixing, the pretreated liquid B was obtained through an MBR device. The parameters of the pretreated liquid B are shown in Table 3. Table 3. Pretreatment solution B index

[0049] Pretreatment solution B enters ozone treatment chamber 4 at a flow rate of 60 g / h. After 24 hours of operation, a culture medium solution for microbial culture is obtained. Relevant indicators are shown in Table 4. Table 4. C-index of culture medium solution

[0050] As can be seen from Tables 2 and 3, the COD, nitrogen and phosphorus, color, and other indicators of pretreated liquid B showed significant changes. Most of the organic suspended solids in the wastewater were rapidly intercepted by the MBR equipment after flocculation, while water-soluble nitrogen and phosphorus elements and other beneficial elements were retained. The color also changed from grayish-black to clear tea color (see Table 3). Figure 14 (Figures (a)-(b) in the table) then pass through an ozone reaction tower, where the ozone reaction intensity and time are controlled according to the required indicators to simultaneously complete deodorization, nitrification, sterilization, and secondary decolorization treatments, ultimately yielding the microbial culture medium solution C shown in Table 4, with the color changing from tea-colored to slightly yellow (see Figure 4). Figure 14 (Figures (b)-(c)) show no odor, and the proportion of nitrate nitrogen in the culture medium is 70.71% of the total nitrogen. The obtained microbial culture medium solution is further prepared with appropriate trace elements, such as... Figure 15As shown, the raw material cost of the culture medium used to produce Rhodopseudomonas palustris (photosynthetic bacteria) for aquaculture is reduced by nearly 80% compared to commercially available fertilizer-type culture media, resulting in significant economic benefits.

[0051] Application Example 2: A large-scale pig farming enterprise in China centrally treats manure from multiple pig farms through a supporting energy biogas plant. The anaerobic fermentation liquid (biogas slurry) is separated into solid and liquid components and then enters an oxidation pond. The supernatant from the oxidation pond is now being treated. The relevant indicators are shown in Table 5. Table 5. Organic Anaerobic Fermentation Broth A Index

[0052] The above-mentioned organic anaerobic fermentation broth A was treated using the method of Example 1 and the equipment of Example 4. The organic anaerobic fermentation broth A was fed into the equipment, and 0.7 kg of polyaluminum chloride flocculant was added per ton of organic anaerobic fermentation broth A. After thorough mixing, the pretreated liquid B was obtained through an MBR device. The parameters of the pretreated liquid B are shown in Table 6. Table 6. Pretreatment solution B index

[0053] Pretreatment solution B enters ozone treatment chamber 4 at a flow rate of 120 g / h. After 24 hours of operation, a culture medium solution for microbial culture is obtained. Relevant indicators are shown in Table 7. Table 7. C-index of culture medium solution

[0054] As can be seen from Tables 6 and 7, the various indicators of pretreatment solution B showed significant changes, and the color changed from grayish-black to clear tea color (see Table 6). Figure 16 (See Figures (a)-(b)). However, due to the incomplete anaerobic fermentation in the energy-type biogas station, odorous substances such as indole and volatile phenols still exist in the fermentation liquid, which cannot be intercepted by the MBR system. At this time, the treated liquid still has a slight odor. After passing through the ozone reaction tower, the ozone treatment intensity and time need to be increased to simultaneously complete deodorization, nitrification, sterilization, and secondary decolorization treatment. Finally, the microbial culture medium liquid C in Table 7 is obtained, and the color changes from tea color to slightly yellow (see Figure 7). Figure 16 (Figures (b)-(c)) show no odor, and the proportion of nitrate nitrogen in the culture medium is 80.48% of the total nitrogen. The obtained microbial culture medium solution, after appropriate reconstitution, is as follows: Figure 17 As shown, this method is used for the production of Chlorella and Spirulina. Compared with commercially available culture media, such as BG11 medium, the raw material cost is reduced by nearly 70%, resulting in significant economic benefits.

Claims

1. An apparatus for preparing microbial culture medium from organic anaerobic fermentation broth, characterized in that, The equipment includes a housing (1), and a mixing chamber (2) for mixing the reagent and the organic anaerobic fermentation liquid, which are separated from left to right by vertically arranged partitions, a sludge chamber (3) for collecting concentrated water, an ozone treatment chamber (4) for treating the purified water with ozone, an MBR device (5) for filtering and removing impurities from the organic anaerobic fermentation liquid, and a dosing pipe (6) for controlling the amount of reagent added to the organic anaerobic fermentation liquid. The MBR equipment (5) is mounted above the sludge tank (3). An inlet for connecting to the dosing pipe (6) is provided on one side of the mixing tank (2). A controlled-release device is provided on the dosing pipe (6). An outlet is provided on the partition on the other side of the mixing tank (2), which connects to the vertical pipe (57) of the MBR equipment (5) via an inclined pipe (58). An ozone reaction tower is installed inside the ozone treatment chamber (4). The inlet of the ozone reaction tower is connected to the clean water outlet (511) of the MBR equipment (5) through a pipe. The concentrated water outlet (512) at the bottom of the MBR equipment (5) corresponds to the position of the sludge chamber (3). The sludge tank (3) is equipped with a filter press, and the sludge tank (3) is equipped with a tailwater outlet that is connected to the dosing pipe (6) via a pipeline. The MBR equipment (5) includes a housing (51), and a plurality of tubular membranes (52) and a suction pump assembly disposed within the housing (51); The shell (51) is composed of a cylindrical part at the top and a conical part at the bottom. The upper part of the cylindrical part is provided with a first flow control tube assembly (53) for connecting the upper end of the tubular membrane (52) and connecting to the outlet of the organic anaerobic fermentation liquid. The lower part of the cylindrical part is provided with a second flow control tube assembly (54) for connecting the lower end of the tubular membrane (52) and controlling the concentrated water to fall into the conical part. The first flow control tube assembly (53) is connected to the inlet of the vertical pipe (57) provided on the shell (51). The bottom of the conical part is provided with a concentrated water outlet (512). The suction pump assembly includes a multi-port suction pipe (55) sleeved on several tubular membranes (52), and a suction pump (551) whose inlet end is connected to the multi-port suction pipe (55). The outlet end of the suction pump (551) is connected to the clean water outlet (511) provided on the housing (51) through a flexible hose with a length margin. Each port of the multi-port suction pipe (55) has an opening and the upper and lower ends of the port are rounded. The inner wall of the housing (51) is provided with a slide rail that is slidably connected to the multi-port suction pipe (55).

2. The apparatus for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 1, characterized in that, The mixing chamber (2) between the inlet and outlet is provided with multiple vertically intersecting baffles (21) to form an S-shaped flow path. Multiple mixers are provided in the mixing chamber (2).

3. The apparatus for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 1, characterized in that, A drive assembly (7) is provided on the side wall of the cylindrical part located on the side of the liquid inlet for reciprocating drive of the suction pump assembly to move up and down using the flow of organic anaerobic fermentation liquid. The drive assembly (7) includes a crank rod (71) rotatably disposed on the inner wall of the cylindrical part, a water wheel (72) disposed in the vertical pipe (57) and one end of which is fixedly connected to the center end of the crank rod (71) by a shaft, and a carrier plate (73) slidably disposed on the inner wall of the cylindrical part. One end of the carrier plate (73) is provided with a guide rail (74) perpendicular to it. The guide rail (74) is slidably connected to the eccentric end of the crank rod (71) by a slider (711). The carrier plate (73) is detachably connected to the multi-port suction pipe (55). A control gear (75) is rotatably provided on the side wall of the cylindrical part located on the side of the liquid inlet. The carrier plate (73) has a tooth segment (731) that meshes with the control gear (75). One end of the second flow control tube assembly (54) is rotatably provided with a control rod (76) for rotating control of the opening of the second flow control tube assembly (54). The control rod (76) is provided with a groove (77), and the control gear (75) is provided with a paddle block (78) for cooperating with the groove (77) to paddle the control rod (76) to rotate. A water blocking plate is provided in the housing (51) located below the purified water outlet (511), and the outlet of the second flow control tube assembly (54) passes through the water blocking plate and connects to the concentrated water outlet (512). The tubular membrane (52) is provided with a magnetic ring (8) that can move up and down. The magnetic ring (8) is connected to the first flow control tube assembly (53) through a rod assembly. The multi-port suction tube (55) is provided with a magnetic block (81) that interacts with the magnetic ring (8). The rod assembly consists of a rod body (82) and several protrusions (83) on the rod body (82). The magnetic ring (8) is slidably sleeved on the rod body (82). The magnetic ring (8) is provided with a torsion spring striking block that cooperates with the protrusions (83). The side wall of the magnetic ring (8) has several bristles.

4. The apparatus for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 3, characterized in that, The other end of the water wheel (72) is sealed through the pipe by rotating the shaft, and the pipe is equipped with a motor connected to the shaft through the output shaft; the multi-port suction pipe (55) is equipped with a flushing pipe (56) for backwashing the tubular membrane (52), and the flushing pipe (56) is connected to the water pump of the external water tank through a flexible hose with a length margin.

5. The apparatus for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 1, characterized in that, The outlet of the ozone reaction tower is equipped with a filter tube (10) filled with activated carbon.

6. The apparatus for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 1, characterized in that, The box (1) is covered with a cover (9), and the bottom surface of the box (1) is provided with casters, and the side wall of the magnetic ring (8) is surrounded by several bristles.

7. A method for preparing microbial culture medium from organic anaerobic fermentation broth, characterized in that, Using any of the devices described in claims 1-6, the method involves adding and mixing the organic anaerobic fermentation liquid with controlled amounts of chemicals to form a suspended organic matter polymer, which is then rapidly intercepted and treated by a matching MBR device (5). The water-soluble organic nutrients in the organic anaerobic fermentation liquid are retained, and the purified water is introduced into the ozone reaction tower for simultaneous deodorization, sterilization, nitrification, and decolorization treatment, ultimately obtaining a culture medium solution for microbial culture. The COD of the organic anaerobic fermentation broth is 3000mg / L-7000mg / L; the amount of the controlled-dosing agent added per ton of organic anaerobic fermentation broth is 0.5kg-5kg; and the organic nutrients are nitrogen, phosphorus, and potassium.

8. The method for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 7, characterized in that, The concentrated water produced by the MBR equipment (5) is quickly introduced into the sludge tank (3). The concentrated water is filtered by a filter press to obtain tailwater and sludge. The tailwater is then combined with organic anaerobic fermentation liquid for recycling. At the same time, the sludge is treated to obtain organic fertilizer.

9. The method for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 7, characterized in that, The method includes the following steps: Step 1: Pass the organic anaerobic fermentation broth into the dosing tube (6), and add the dosing agent in a controlled manner through the dosing tube (6) to carry out the mixing reaction and form a suspended organic matter polymer. Step 2: The organic anaerobic fermentation liquid after step 1 is introduced into the MBR device (5). The suspended organic matter polymers that meet the filtration accuracy of the MBR device (5) are quickly intercepted. While greatly reducing the COD, SS, turbidity and color of the fermentation liquid, the water-soluble organic nutrients in the fermentation liquid are retained. The purified water and concentrated water are obtained by rapid treatment through the MBR device (5). Step 3: The concentrated water obtained in step 2 is introduced into the sludge tank (3), and the concentrated water is filtered by a filter press to obtain tailwater and sludge. The tailwater is introduced into the dosing pipe (6) to merge with the organic anaerobic fermentation liquid, and the sludge is treated to obtain organic fertilizer. Step 4: The purified water obtained in Step 2 is introduced into the ozone reaction tower for simultaneous deodorization, sterilization, nitrification and decolorization treatment, and finally a culture medium solution for microbial culture is obtained.

10. The method for preparing microbial culture medium from organic anaerobic fermentation broth as described in claim 7, characterized in that, The controlled dosage of the added agent is polyaluminum chloride flocculant; the dosage of the added agent is controlled according to the filtration accuracy of the MBR equipment (5) and the nutritional indicators of the culture medium solution; the ozone flow rate of the ozone reaction tower is 50~200g / h.

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