System device, method and use for quantitative sample injection of a chip detection sample

Abstract

The application provides a chip detection sample quantitative injection system device, method and use. The system device is used for injecting quantitative detection sample into a gap cavity of a chip. The system device comprises a sample quantitative assembly and a liquid injection chamber. The sample quantitative assembly comprises a body, and a quantitative cavity is arranged in the body. The liquid injection chamber comprises an open shell, and a partition plate is arranged in the shell. The partition plate divides the shell into a first cavity and a second cavity. The detection sample is injected into the first cavity. The sample quantitative assembly is placed in the liquid injection chamber and continuously pressed down. The body is inserted into the first cavity to quantitatively inject the sample. The application does not need an additional quantitative tool, quantitatively injects the detection sample, realizes the automation of chip injection, reduces the dependence on the additional quantitative tool, and makes the operation more convenient and flexible.

Description

Technical Field

[0001] This application belongs to the field of microfluidic chip technology, and relates to quantitative sample injection within a chip, particularly to a system, method and application for chip-based sample quantitative injection. Background Technology

[0002] In the polymerase chain reaction (PCR) process, the test sample needs to be added by the tester when using the chip after sample collection and processing. Usually, the test sample is collected in the collection tube, and the amount of sample to be injected into the digital microfluidic chip is fixed. If the sample is quantitatively taken from the collection tube by liquid quantitative tools such as pipettes and droppers and then injected into the chip sample inlet, it increases the user's operation steps and greatly limits the application scenarios of digital microfluidic chips.

[0003] CN109652298A discloses a droplet PCR amplification and detection device based on a microfluidic chip. The device includes a droplet microfluidic chip, an XYZ motion unit, a PCR amplification unit, and a detection unit. After a droplet containing DNA molecules is generated, it is introduced into the droplet microfluidic chip using a pipette, and the chip is placed in the droplet PCR amplification unit for PCR amplification reaction.

[0004] CN107831811A discloses a microfluidic flow control device and method for micro / nanocellulose. The device includes: an injection pump, an injection section, a sample plug, a microfluidic chip, and a sealing membrane. The injection pump quantitatively controls the flow rate of the injected micro / nanocellulose suspension. The injection section is used to inject the suspension containing micro / nanocellulose into the microfluidic chip.

[0005] CN112271416A discloses a lithium battery electrolyte injection device and manufacturing method that uses a storage cup to inject electrolyte. The device includes a battery casing, a mold holder, and a storage cup. A locking component is provided between the storage cup and the mold holder for detachable connection. A sealed injection cavity is formed between the storage cup and the top of the battery casing by the locking component. The storage cup contains a storage cavity for storing a fixed amount of electrolyte. The electrolyte in the storage cavity can be pressurized by external pressure to enter the injection cavity first, and then injected into the battery casing in one go through the injection hole.

[0006] Traditional PCR reactions cannot be modified midway once started, and the fully manual operation is time-consuming and labor-intensive. Digital microfluidic chips use the principle of electrowetting technology, which modulates the surface energy of solid and liquid through electrical potential, and uses the imbalance of surface energy to drive the liquid to move, thereby achieving precise control of microfluidics. However, this method is highly dependent on the syringe pump and is therefore costly. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this application provides a system, method, and application for quantitative injection of chip detection samples. During use, the built-in sample quantification component can complete the quantitative extraction of the required detection sample without the need for additional quantification tools. This enables the quantitative injection of the detection sample, thereby automating chip sample injection, reducing reliance on additional quantification tools, and making the operation more convenient and flexible.

[0008] The following technical solution is adopted in this application:

[0009] In a first aspect, this application provides a system device for quantitative injection of chip detection samples, used to inject a quantitative detection sample into the gap cavity of a chip. The system device includes a sample quantitative component and an injection chamber. The sample quantitative component includes a body, and the body has a quantitative chamber. The injection chamber includes an open shell, and the shell has a partition that divides the shell into a first chamber and a second chamber. The detection sample is injected into the first chamber. The sample quantitative component is placed in the injection chamber and continuously pressed down. The body extends into the first chamber to perform quantitative injection.

[0010] The chip detection sample quantitative injection system provided in this application can complete the quantitative extraction of the required detection sample through its built-in sample quantitative component during use, without the need for additional quantitative tools, and quantitatively inject the detection sample, thereby realizing the automation of chip injection, reducing the dependence on additional quantitative tools, and making the operation more convenient and flexible.

[0011] In this application, the dimensions of the shell and the body can be adjusted to meet the sample volume requirements of different systems, thereby achieving the function of quantitative injection of larger or smaller liquid volumes.

[0012] It should be noted that the first chamber provided in this application serves as a quantitative sample injection chamber, while the second chamber serves as an overflow storage chamber. This application does not impose specific limitations or special requirements on the structure and combination of the partition, the first chamber, and the second chamber. For example, when the partition is a hollow column, the shell is divided into a first chamber inside the column and a second chamber outside the column, wherein the first chamber and the second chamber are in a containment relationship, and the injection column is located inside the first chamber. When the partition is a vertical plate, the shell is divided into a first chamber and a second chamber arranged side by side, and a notch can be provided at the connection between the first chamber and the second chamber to achieve the overflow of the detection sample.

[0013] As a preferred technical solution of this application, at least one injection column is provided in the first chamber, which is connected to the gap cavity. During the quantitative injection process, the sample quantitative component is continuously pressed down, and the injection column extends into the quantitative chamber.

[0014] The injection column in this application can be integrally formed with the shell, or it can be a separate component assembled at the bottom of the shell.

[0015] Preferably, the injection chamber further includes an injection channel that extends through the injection column, and the injection channel is connected to the gap cavity of the chip.

[0016] It should be noted that this application does not impose specific limitations or special requirements on the structure of the injection channel. For example, a straight or oblique hole that penetrates the injection column can be selected, or tubular fittings can be assembled inside the injection column.

[0017] Preferably, the injection channel has a guide notch at one end near the gap cavity.

[0018] Preferably, the inner wall of the housing near the open end is provided with an exhaust groove.

[0019] It should be noted that the length of the exhaust groove in this application is matched with the height of the body, so as to ensure that the exhaust ends when the sample quantification component is pressed down to the bottom of the shell, so that the overflowing liquid is sealed in the second chamber.

[0020] As a preferred technical solution of this application, a sealing element is provided at one end of the body that extends into the housing.

[0021] It should be noted that during the quantitative injection process, the main body extends into the first chamber and forms a sealed environment with the first chamber through the sealing element, so that the liquid can only flow out from the injection channel after being squeezed.

[0022] Preferably, a groove is formed on the outer wall of the body, and the sealing element is disposed in the groove.

[0023] Preferably, the sealing element is an O-ring.

[0024] It should be noted that at the beginning of the sample injection in this application, the sample quantification component is slowly and uniformly lowered under downward pressure. When the "O" ring begins to enter the first chamber, the excess test sample in the first chamber is squeezed by the body and overflows into the second chamber. As it continues to descend until the "O" ring completely enters the first chamber, a sealed environment is formed, and the amount of test sample in the first chamber remains basically constant. With continuous downward pressure, the test sample enters the injection channel and enters the chip gap cavity. When the body is pressed down to the bottom of the first chamber, the quantitative sample injection is completed.

[0025] The quantitative component in this application can be pre-stored in the empty area of ​​the chip shell or packaged together with the chip, and placed on the injection chamber after the initial drop of the test sample.

[0026] As a preferred technical solution of this application, the sample quantification component further includes a base for fixing the body. During the sample injection process, the edge of the base is press-fitted with the inner wall of the shell to achieve a seal.

[0027] It should be noted that in this application, the sample quantification component with the assembled seal is placed in the injection chamber, and it must be placed horizontally. The base of the sample quantification component has an interference fit with the inner wall of the shell during the pressing process, which plays a secondary sealing role.

[0028] Preferably, the base is further provided with a guide on the surface near the body.

[0029] Preferably, a gap is left between the body and the guide, and during the injection process, the partition gradually extends into the gap.

[0030] It should be noted that the guide in this application enables the sample quantification component to descend vertically, avoiding deviation or tilting of the body that could lead to injection errors or jamming and inability to press down.

[0031] Preferably, the base surface has an exhaust vent.

[0032] Preferably, the outer edge of the base is provided with an exhaust notch.

[0033] It should be noted that those skilled in the art can choose to provide venting by providing venting holes on the surface of the base and / or providing venting notches on the outer edge of the base, depending on the specific circumstances.

[0034] As a preferred embodiment of this application, the injection column is higher than the partition.

[0035] Preferably, a gap is left between the outer wall of the injection column and the inner wall of the quantitative chamber, and the test sample flows within the gap.

[0036] As a preferred technical solution of this application, the side surface of the partition near the sample quantification component is provided with a stepped groove.

[0037] It should be noted that the stepped groove at the top of the partition in this application serves as a buffer.

[0038] Preferably, the stepped groove is divided into a first groove and a second groove along the direction in which the body extends into the first chamber. The width of the first groove is greater than the width of the second groove, and the test sample is located at the junction of the first groove and the second groove.

[0039] It should be noted that, before the system device provided in this application starts the injection, the test sample is injected into the first chamber of the injection chamber. The amount of test sample is dripped to the junction of the first groove and the second groove. At this time, since the injection column is higher than the first chamber, the liquid cannot enter the injection channel temporarily. Then, during the injection process, the sample quantification component is pressed down, and part of the test sample is gradually squeezed between the injection column and the quantification chamber, and then enters the injection channel and flows into the gap cavity.

[0040] Secondly, this application provides a method for quantitative injection of chip detection samples, wherein the method employs the system apparatus described in the first aspect, and the method includes:

[0041] The test sample is injected into the first chamber, the sample quantification component is placed in the injection chamber, and the sample quantification component is continuously pressed down. The main body is sent into the first chamber from the open end of the shell. Part of the test sample in the first chamber overflows into the second chamber, and the test sample entering the quantification chamber flows into the gap cavity to achieve quantitative sample injection.

[0042] As a preferred technical solution of this application, the quantitative sample injection specifically includes:

[0043] As the sample quantification component is continuously pressed down, the injection column gradually extends into the quantification chamber, squeezing the test sample into the gap between the injection column and the quantification chamber, and then flowing into the gap cavity through the injection channel.

[0044] Preferably, the method includes injecting a test sample into the first chamber using a collection tube.

[0045] Preferably, the pressing body includes a motor or lifting mechanism to achieve automated pressing.

[0046] As a preferred technical solution of this application, the method further includes venting during the quantitative injection process.

[0047] Preferably, exhaust is achieved using exhaust vents on the surface of the base.

[0048] Preferably, the base has an exhaust notch on its outer edge for venting.

[0049] Preferably, exhaust is performed using an exhaust groove on the inner wall of the housing.

[0050] For example, the method for quantitative injection of chip detection samples provided in this application specifically includes the following steps:

[0051] (1) Use the collection tube to drip the test sample into the first chamber until the liquid level of the test sample rises to the junction of the first groove and the second groove at the top of the partition. At this time, because the injection column is higher than the partition, the test sample liquid cannot enter the injection channel temporarily.

[0052] (2) After dripping the test sample, place the sample quantification component with the "O" ring assembled horizontally in the injection chamber, with the main body placed close to the injection column;

[0053] (3) The sample quantitative component is pressed down by a motor or lifting mechanism to make it descend slowly and uniformly. The exhaust groove on the inner wall of the shell is used for exhaust. When the “O” ring begins to enter the first chamber, the edge of the base and the inner wall of the shell are pressurized to achieve a seal. The excess test sample in the first chamber is squeezed by the body and overflows into the second chamber. The descent continues until the “O” ring is completely in the first chamber, forming a sealed environment. The partition gradually extends into the gap between the body and the guide. The amount of test sample in the first chamber is basically constant. With continuous pressing, the test sample enters the injection channel and enters the chip gap cavity. When the body is pressed down to the bottom of the first chamber, the quantitative injection is completed.

[0054] Thirdly, this application provides the use of the system device described in the first aspect, the system device being used to inject a detection sample into the gap cavity of a digital microfluidic chip.

[0055] The digital microfluidic chip in this application employs the principle of electrowetting technology. It modulates the surface energy of solids and liquids through electrical potential, and utilizes the imbalance of surface energy to drive liquid movement, thereby achieving precise control of micro-liquids. Its main components include: a transparent conductive cap (e.g., ITO glass), an electrode array with a hydrophobic layer and a dielectric layer on its surface, and a gap cavity between the transparent conductive cap and the electrode array for droplet movement. The surface of the electrode array is equipped with the chip-based sample quantitative injection system provided in this application, and the injection column is connected to the gap cavity through an injection channel.

[0056] The system refers to an equipment system, device system, or production device.

[0057] Compared with the prior art, the beneficial effects of this application are as follows:

[0058] This application provides a system, method, and application for quantitative injection of chip detection samples. During use, the system can extract the required detection samples quantitatively using its built-in sample quantification component, eliminating the need for additional quantification tools. This automates chip injection, reduces reliance on additional quantification tools, and makes operation more convenient and flexible. Attached Figure Description

[0059] Figure 1 This is a schematic diagram of the system device for quantitative injection of chip detection samples provided in Embodiment 1 of this application;

[0060] Figure 2 This is a schematic diagram of the sample quantification component provided in Embodiment 1 of this application;

[0061] Figure 3 This is a schematic diagram of the injection chamber provided in Embodiment 1 of this application;

[0062] Figure 4 A schematic diagram showing the sample quantification component entering the injection chamber as provided in Embodiment 1 of this application;

[0063] Figure 5 This is a schematic diagram of the structure of the digital microfluidic chip provided in Application Example 1 of this application;

[0064] Figure 6 A schematic diagram of the quantitative injection process provided for Application Example 1 of this application.

[0065] The components are as follows: 1-Sample quantification component; 2-Injection chamber; 3-Body; 4-"O" ring seal; 5-Quantification chamber; 6-Guide component; 7-Shell; 8-Separator; 9-First chamber; 10-Second chamber; 11-Injection column; 12-Injection channel; 13-Step groove; 14-Gap cavity; 15-Electrode array; 16-Hydrophobic layer; 17-Dielectric layer; 18-Transparent conductive cap. Detailed Implementation

[0066] It should be understood that in the description of this application, the terms "center," "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. These terms are used solely for the convenience of describing this application and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application. Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0067] It should be noted that, in the description of this application, unless otherwise expressly specified and limited, the terms "set up," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0068] The technical solution of this application will be further described below with reference to the accompanying drawings and specific embodiments.

[0069] In one specific embodiment, this application provides a system device for quantitative injection of chip detection samples. The system device is used to inject a quantitative detection sample into the gap cavity 14 of the chip. The system device includes a sample quantitative component 1 and an injection chamber 2. The sample quantitative component 1 includes a body 3, and the body 3 is provided with a quantitative chamber 5. The injection chamber 2 includes an open shell 7, and the shell 7 is provided with a partition 8. The partition 8 divides the shell 7 into a first chamber 9 and a second chamber 10. The detection sample is injected into the first chamber 9. The sample quantitative component 1 is placed in the injection chamber 2 and continuously pressed down. The body 3 extends into the first chamber 9 to perform quantitative injection.

[0070] In this application, the sample volume requirements of different systems can be met by adjusting the dimensions of the shell 7 and the body 3, thereby achieving the function of quantitative injection of larger or smaller liquid volumes.

[0071] The first chamber 9 provided in this application serves as a quantitative sample injection chamber, and the second chamber 10 serves as an overflow liquid storage chamber. This application does not impose specific limitations or special requirements on the structure and combination of the partition 8, the first chamber 9, and the second chamber 10. For example, when the partition 8 is a hollow column, the shell 7 is divided into the first chamber 9 inside the column and the second chamber 10 outside the column, wherein the first chamber 9 and the second chamber 10 are in an inclusive relationship, and the injection column 11 is located inside the first chamber 9. When the partition 8 is a vertical plate, the shell 7 is divided into the first chamber 9 and the second chamber 10 arranged side by side. A notch can be provided at the connection between the first chamber 9 and the second chamber 10 to realize the overflow of the detection sample.

[0072] Furthermore, at least one injection column 11 communicating with the gap cavity 14 is provided in the first chamber 9. During the quantitative injection process, the sample quantitative component 1 is continuously pressed down, and the injection column 11 extends into the quantitative chamber 5. In this application, the injection column 11 can be integrally formed with the housing 7, or it can be a separate component assembled at the bottom of the housing 7.

[0073] The injection chamber 2 further includes an injection channel 12 that penetrates the injection column 11, and the injection channel 12 connects to the chip's gap cavity 14. This application does not impose specific limitations or special requirements on the structure of the injection channel 12. For example, a straight or oblique hole penetrating the injection column 11 can be selected, or tubular fittings can be assembled inside the injection column 11.

[0074] The injection channel 12 is provided with a guide notch at one end near the gap cavity 14.

[0075] The inner wall of the housing 7 near the open end is provided with an exhaust groove. In this application, the length of the exhaust groove is matched with the height of the body 3, which should ensure that the exhaust ends when the sample quantification component 1 is pressed down to the bottom of the housing 7, so that the overflowing liquid is sealed in the second chamber 10.

[0076] Furthermore, a sealing element is provided at one end of the body 3 that extends into the housing 7. During the quantitative injection process, the body 3 extends into the first chamber 9 and forms a sealed environment with the first chamber 9 through the sealing element, so that the liquid can only flow out from the injection channel 12 after being squeezed.

[0077] The outer wall of the body 3 has a groove, and the sealing element is disposed in the groove.

[0078] The sealing element is an O-ring 4. At the start of sample injection in this application, the sample quantitative component 1 is slowly and uniformly lowered under downward pressure. Initially, before the O-ring has achieved its sealing function, excess liquid in the first chamber 9 is squeezed into the second chamber 10 by the body 3 of the sample quantitative component 1. As it continues to descend until the O-ring begins its first sealing, the liquid volume in the first chamber 9 remains essentially constant. With continued downward pressure, the sample enters the chip's gap cavity 14 through the injection column 11. When the sample quantitative component 1 reaches the bottom, quantitative sample injection is completed.

[0079] The quantitative component in this application can be pre-stored in the empty area of ​​the chip shell or packaged together with the chip, and placed on the injection chamber after the initial drop of the test sample.

[0080] Furthermore, the sample quantification component 1 also includes a base for fixing the main body 3. During the sample injection process, the edge of the base is press-fitted with the inner wall of the housing 7 to achieve a seal. In this application, the sample quantification component 1 with the assembled seal is placed in the injection chamber 2, ensuring that it is placed horizontally. The base of the sample quantification component 1 has an interference fit with the inner wall of the housing 7 during the pressing process, which plays a secondary sealing role.

[0081] The base surface near the main body 3 is also provided with a guide 6. A gap is left between the main body 3 and the guide 6, and during the sample injection process, the partition 8 gradually extends into the gap. The guide 6 in this application allows the sample quantitative component 1 to descend vertically, avoiding deviation or tilting of the main body 3 that could cause injection errors or jamming that would prevent downward pressure.

[0082] The base surface has ventilation holes.

[0083] The base has an exhaust vent on its outer edge.

[0084] Furthermore, the injection column 11 is higher than the partition 8.

[0085] A gap is left between the outer wall of the injection column 11 and the inner wall of the quantitative chamber 5, and the test sample flows within the gap.

[0086] Furthermore, a stepped groove 13 is formed on the surface of the partition 8 near the sample quantification component 1. The stepped groove 13 is divided into a first groove and a second groove along the direction in which the body 3 extends into the first chamber 9. The width of the first groove is greater than the width of the second groove, and the sample is located at the junction of the first groove and the second groove.

[0087] Before the injection begins, the system device provided in this application injects the test sample into the first chamber 9 of the injection chamber 2. The test sample is dripped to the junction of the first groove and the second groove. At this time, because the injection column 11 is higher than the first chamber 9, the liquid cannot enter the injection channel 12. Subsequently, during the injection process, the sample quantification component 1 is pressed down, and part of the test sample is gradually squeezed between the injection column 11 and the quantification chamber 5, and then enters the injection channel 12 and flows into the gap chamber 14.

[0088] In another specific embodiment, this application provides a method for quantitative injection of chip detection samples. The method employs the system apparatus described in the first aspect, and the method includes:

[0089] The test sample is injected into the first chamber 9. The sample quantitative component 1 is placed in the injection chamber 2. The sample quantitative component 1 is continuously pressed down. The body 3 is sent into the first chamber 9 from the open end of the shell 7. Part of the test sample in the first chamber 9 overflows into the second chamber 10. The test sample that enters the quantitative chamber 5 flows into the gap cavity 14 to achieve quantitative injection.

[0090] Furthermore, the quantitative sample injection specifically includes:

[0091] As the sample quantification component 1 is continuously pressed down, the injection column 11 gradually extends into the quantification chamber 5, squeezing the sample into the gap between the injection column 11 and the quantification chamber 5, and then flowing into the gap cavity 14 through the injection channel 12.

[0092] The method involves injecting a test sample into a first chamber 9 using a collection tube. The test sample is located at the junction of the first groove and the second groove.

[0093] The pressing body 3 includes a motor or lifting mechanism to achieve automated pressing.

[0094] Furthermore, the method also includes venting during the quantitative injection process.

[0095] Exhaust is achieved through vent holes on the surface of the base.

[0096] The base has an exhaust vent on its outer edge for ventilation.

[0097] Exhaust is achieved by using an exhaust groove on the inner wall of the housing 7.

[0098] Example 1

[0099] This embodiment provides a system device for quantitative injection of chip-based detection samples, such as... Figure 1 As shown, the system device includes a sample quantification component 1 and an injection chamber 2, such as... Figure 2 As shown, the sample quantification component 1 includes a body 3, which has a quantification chamber 5 inside. A groove is formed on the outer wall of the body 3, and an O-ring seal 4 is placed in the groove. The sample quantification component 1 also includes a base for fixing the body 3. During the sample injection process, the edge of the base is press-fitted with the inner wall of the housing 7 to achieve a seal. Four guide members 6 are also provided on the side surface of the base near the body 3, and a gap is left between the body 3 and the guide members 6.

[0100] like Figure 3 As shown, the injection chamber 2 includes an open shell 7. Inside the shell 7 is a partition 8, which is a hollow cylinder. The partition 8 divides the shell 7 into a first chamber 9 and a second chamber 10. The first chamber 9 contains a sample injection column 11 that communicates with the gap cavity 14. The injection chamber 2 also includes an injection channel 12 that penetrates the sample injection column 11. The injection channel 12 connects to the gap cavity 14 of the chip. A guide notch is provided at one end of the injection channel 12 near the gap cavity 14. Figure 3 As shown, the inner wall of the shell 7 near the open end is provided with an exhaust groove.

[0101] like Figure 4 As shown, the injection column 11 is higher than the partition 8, and there is a gap between the outer wall of the injection column 11 and the inner wall of the quantitative chamber 5, so that the detection sample flows within the gap.

[0102] The partition 8 has a stepped groove 13 on the side surface near the sample quantification component 1. The stepped groove 13 is divided into a first groove and a second groove along the direction of the body 3 extending into the first chamber 9. The width of the first groove is greater than the width of the second groove. The sample is located at the junction of the first groove and the second groove.

[0103] Application Example 1

[0104] In this application example, the chip detection sample quantitative injection system device provided in Example 1 is used to... Figure 5 The digital microfluidic chip shown is injected with reagents into its cavity 14. The main components of the digital microfluidic chip in this application example include: a transparent conductive cap 18 and an electrode array 15 with a surface containing a hydrophobic layer 16 and a dielectric layer 17. A cavity 14 for droplet movement is provided between the transparent conductive cap 18 and the electrode array 15. The surface of the electrode array 15 is equipped with the chip-based sample quantitative injection system provided in this application. The injection column 11 is connected to the cavity 14 via an injection channel 12.

[0105] like Figure 6As shown, quantitative sample injection specifically includes the following steps:

[0106] (1) Use the collection tube to drip the test sample into the first chamber 9 until the liquid level of the test sample rises to the junction of the first groove and the second groove at the top of the partition 8. At this time, because the injection column 11 is higher than the partition 8, the test sample liquid cannot enter the injection channel 12 temporarily.

[0107] (2) After dripping the test sample, place the sample quantification component 1 with the "O" ring assembled horizontally in the injection chamber 2, with the main body 3 placed close to the injection column 11;

[0108] (3) The sample quantitative component 1 is pressed down by a motor or lifting mechanism to make it descend slowly and uniformly. The exhaust groove on the inner wall of the housing 7 is used for exhaust. When the “O” ring begins to enter the first chamber 9, the edge of the base and the inner wall of the housing 7 are pressurized to achieve a seal. The guide 6 extends into the second chamber 10. The excess test sample in the first chamber 9 is squeezed by the body 3 and overflows into the second chamber 10. The descent continues until the “O” ring is completely in the first chamber 9, forming a sealed environment. The partition 8 gradually extends into the gap between the body 3 and the guide 6. The amount of test sample in the first chamber 9 is basically constant. With continuous pressing, the test sample enters the injection channel 12 and enters the chip gap cavity 14. When the body 3 is pressed down to the bottom of the first chamber 9, the quantitative injection is completed.

[0109] (1) The sealing performance and injection accuracy of sample quantitative component 1 are tested, specifically using the following steps:

[0110] 1. Weigh the EP tubes, label them with serial numbers, and record the weight;

[0111] 2. Measure the key dimensions of the monomer and sample quantitative component 1 and label them with serial numbers;

[0112] 3. Use a pipette to inject liquid into sample quantification component 1, then place the sample quantification stopper with O-ring on top, and use an EP tube to collect the injected liquid below. Manually press down, weigh the EP tube that collects the liquid, record the data, and calculate the difference. The results are shown in Table 1.

[0113] 4. After the experiment, tidy up the lab bench.

[0114] Table 1

[0115]

[0116]

[0117] (2) Stress test of sample quantification component 1, specifically using the following steps:

[0118] 1. The injection chamber 2 is placed directly below the press head of the press;

[0119] 2. Inject sample liquid into injection chamber 2 until it reaches the vicinity of stepped groove 13 (the liquid level is located at the junction of the first groove and the second groove);

[0120] 3. Place the sample quantification assembly 1 with the O-rings assembled above the injection chamber 2;

[0121] 4. Start the press and press down;

[0122] 5. Observe and record the pressure value when pressing down. The results are shown in Table 2.

[0123] Table 2

[0124]

[0125]

[0126] As shown in Table 1, the range of the forty test samples is 12.8 μL, indicating that the injection accuracy meets the requirements. The sample quantitative component 1 provided in this application has good sealing performance, and the test sample can be injected into the gap cavity 14 of the chip from the injection channel 12 as designed. As shown in Table 2, the average pressure required for the sample quantitative component 1 is 145.975 N, the maximum pressure is 158.7 N, and the minimum pressure is 131.8 N.

[0127] The chip detection sample quantitative injection system device provided in this application can complete the quantitative extraction of the required detection sample through its built-in sample quantitative component 1 during use, without the need for additional quantitative tools, and quantitatively inject the detection sample, thereby realizing the automation of chip injection, reducing the dependence on additional quantitative tools, and making the operation more convenient and flexible.

[0128] The applicant declares that the above description is only a specific implementation of this application, but the protection scope of this application is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in this application fall within the protection and disclosure scope of this application.

Claims

1. A system apparatus for quantitative injection of a chip detection sample, used to inject a quantitative detection sample into the gap cavity of a chip, the system apparatus comprising: A sample quantification component, comprising a body, wherein a quantification chamber is provided within the body; The injection chamber includes an open shell with a partition inside, which divides the shell into a first chamber and a second chamber. The first chamber is used to contain the injected test sample. The sample quantification component is used to press into the injection chamber so that the body extends into the first chamber for quantitative sample injection; The first chamber is provided with at least one injection column that communicates with the gap cavity, and the at least one injection column is used to extend into the quantitative chamber; when the injection column extends into the quantitative chamber, a gap is left between the outer wall of the injection column and the inner wall of the quantitative chamber; The injection column is higher than the partition.

2. The system apparatus according to claim 1, wherein, The inner wall of the shell near the open end is provided with an exhaust groove.

3. The system apparatus according to claim 1, wherein, The outer wall of the body is provided with a sealing element.

4. The system apparatus according to claim 3, wherein, The outer wall of the body is provided with a groove, and the sealing element is disposed in the groove.

5. The system apparatus according to claim 3, wherein, The sealing element is an "O" ring.

6. The system apparatus according to claim 1, wherein, The sample quantification component also includes a base for fixing the body, the edge of which is used to make an interference fit with the inner wall of the housing during the sample injection process.

7. The system apparatus according to claim 6, wherein, The base is also provided with a guide on the surface near the main body.

8. The system apparatus according to claim 7, wherein, A gap is left between the body and the guide member, and the gap is used to accommodate the partition during the injection process.

9. The system apparatus according to claim 6, wherein, The base surface has ventilation holes.

10. The system apparatus according to claim 6, wherein, The base has an exhaust vent on its outer edge.

11. The system apparatus according to claim 1, wherein, The sample quantification component also includes a base for fixing the body, the edge of the base being used for interference fit with the inner wall of the housing during sample injection, the outer wall of the body being provided with a sealing element, and the injection chamber also includes an injection channel penetrating the injection column, the injection channel being used to connect to the gap cavity of the chip.

12. The system apparatus according to claim 11, wherein, The injection channel has a guide notch at one end near the gap cavity.

13. The system apparatus according to any one of claims 1-12, wherein, The partition has a stepped groove on the side surface near the sample quantification component.

14. The system apparatus according to claim 13, wherein, The stepped groove includes a first groove and a second groove in the direction along which it extends into the first chamber from the body, wherein the width of the first groove is greater than the width of the second groove.

15. A method for quantitative injection of samples for chip detection, the method employing the system apparatus according to any one of claims 1-14, the method comprising: The test sample was injected into the first chamber; Place the sample quantification component in the injection chamber; The sample quantification component is continuously pressed down so that the main body is sent into the first chamber from the open end of the shell. Part of the test sample in the first chamber overflows into the second chamber, and the test sample entering the quantification chamber flows into the gap cavity to achieve quantitative sample injection.

16. The method according to claim 15, wherein, The first chamber is provided with at least one injection column communicating with the gap chamber. The injection column includes a through injection channel communicating with the gap chamber. The quantitative injection includes: continuously pressing down the sample quantitative component, the injection column gradually extending into the quantitative chamber, squeezing the test sample into the gap between the injection column and the quantitative chamber, so that the test sample flows into the gap chamber through the injection channel.

17. The method according to claim 16, wherein, The method includes injecting a test sample into the first chamber using a collection tube.

18. The method according to claim 17, wherein, The injected test sample is located at the junction of the first and second grooves on the surface of the partition near the sample quantification component.

19. The method of claim 16, wherein, The method also includes using a motor or lifting mechanism to press down the sample quantification component.

20. The method according to any one of claims 16-19, wherein, The method also includes venting during the quantitative injection process.

21. The method according to claim 20, wherein, Exhaust is achieved through venting holes on the base surface of the sample quantification component.

22. The method according to claim 20, wherein, The venting is achieved by using the venting notch on the outer edge of the base of the sample quantification component.

23. The method of claim 20, wherein, Exhaust is achieved using a long exhaust groove on the inner wall of the casing.

24. Use of a system device according to any one of claims 1-14, the system device being used to inject a detection sample into a gap cavity of a digital microfluidic chip.

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