一种松树与干巴菌形成菌根苗的方法
By using mechanical scraping and light cultivation under optimized culture conditions, the problem of symbiosis between pine trees and *Gynostemma pentaphyllum* was solved, improving the survival rate and infection rate of pine seedlings and enhancing the stress resistance and disease prevention ability of pine trees.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHAANXI UNIV OF SCI & TECH
- Filing Date
- 2024-04-30
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies make it difficult to efficiently establish a symbiotic relationship between pine trees and *Gnaphalium affine* under artificial conditions, resulting in a low survival rate of transplanted pine seedlings. Furthermore, existing treatment solutions are not conducive to the infecting fungi.
The roots of pine seedlings were mechanically scraped and immersed in a culture solution of *Gynostemma pentaphyllum* mycelium. They were then cultured under light in a semi-solid culture medium. With optimized culture medium composition and environmental conditions, *Gynostemma pentaphyllum* mycelium symbiosis was formed. Subsequently, the seedlings were hardened off and transplanted to the field.
Stable symbiosis between pine seedlings and *Gynostemma pentaphyllum* was achieved, increasing the survival rate of symbiotic seedlings to 80%-90% and the infection rate to 70%-85%, thereby enhancing the stress resistance and disease prevention ability of pine trees.
Smart Images
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