A method for preparing and using a hydrophilic interaction chromatography stationary phase of acryloyloxyethyltrimethylammonium chloride bonded silica gel

Abstract

The application relates to a preparation method and application of an acryloxyethyl trimethyl ammonium chloride bonded silica gel hydrophilic chromatographic stationary phase. The hydrophilic chromatographic stationary phase is prepared by bonding DAC to the surface of mercaptopropyl modified silica gel microspheres through a "thiol-alkene" click chemistry reaction. The method comprises the following steps: (1) drying of silica gel microspheres; (2) bonding of a silanization modification reagent, mercaptopropyl triethoxysilane, to the surface of silica gel to obtain mercaptopropyl silica gel; (3) bonding of DAC with a vinyl group to the surface of silica gel modified with mercapto through a "thiol-alkene" click chemistry reaction to prepare a novel DAC bonded silica gel hydrophilic chromatographic mode stationary phase. The preparation method is simple, low in cost, good in selectivity and mild in reaction conditions; the stationary phase is good in bonding force, not easy to flow out, good in stability and repeatability; and the stationary phase is good in separation effect on polar compounds.

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