Test sample loading device and nucleic acid detection integrated machine with it

Abstract

This invention provides a analyte loading device and a nucleic acid detection integrated machine having the same. The analyte loading device includes: a storage mechanism including a storage chamber for storing storage tubes; a transfer mechanism including a transfer component and a detection component disposed on the transfer component; a movable frame having a first bearing position and a second bearing position spaced apart for placing storage tubes, wherein when the movable frame moves to the loading position, the transfer component can transfer the storage tubes between the movable frame and the storage chamber to load the storage tubes onto the first bearing position and / or the second bearing position; a capping mechanism including a capping component capable of opening and closing the storage tubes, wherein when the movable frame moves to the capping position, the first bearing position of the movable frame corresponds to the capping component; and a dispensing mechanism including a dispensing component capable of dispensing the stored material from the storage tubes into a reaction container. The technical solution provided by this application can solve the problem of low transport efficiency of storage tubes in the transfer mechanism of existing nucleic acid detection equipment.

Description

Technical Field

[0001] This invention relates to the field of nucleic acid detection technology, and more specifically, to a test sample loading device and an integrated nucleic acid detection machine having the same. Background Technology

[0002] In clinical medical testing laboratories, collected samples are usually stored in storage tubes, and then nucleic acid testing equipment is used to test the samples in the storage tubes.

[0003] In the existing technology, storage tubes are mainly divided into two categories. The first type of storage tube has a cap, which needs to be opened using a capping device before filling. The second type of storage tube does not have a cap, so it does not need to be opened before filling. This results in low transport efficiency of the storage tubes by the transmission mechanism of the nucleic acid detection equipment in the existing technology when the two types of storage tubes are used together. Summary of the Invention

[0004] This invention provides a test sample loading device and a nucleic acid detection integrated machine having the same, to solve the problem of low transport efficiency of storage tubes in the transmission mechanism of existing nucleic acid detection equipment.

[0005] According to one aspect of the present invention, a test substance loading device is provided, comprising: a storage mechanism including a storage chamber for storing a storage tube; a transfer mechanism including a transfer member and a detection member disposed on the transfer member; a transmission mechanism including a movable frame movably disposed thereon, the movable frame having a first bearing position and a second bearing position for placing the storage tube spaced apart, the movable frame having a loading position, an opening position and a filling position, the movable frame being movable between the loading position, the opening position and the filling position, when the movable frame moves to the loading position, the movable frame is located at a position corresponding to the storage mechanism, and the transfer member is capable of transferring the storage tube between the movable frame and the storage chamber to load the storage tube onto the first bearing position and / or the second bearing position; an opening mechanism including an opening member capable of opening and closing the storage tube, when the movable frame moves to the opening position, the first bearing position of the movable frame corresponds to the opening member; and a filling mechanism including a filling member capable of filling the storage substance in the storage tube into a reaction vessel, when the movable frame moves to the filling position, the movable frame is located at a position corresponding to the filling mechanism.

[0006] Further, the cover opening component includes: a base; a cap pressure post disposed on the base and protruding from the lower surface of the base, wherein when the moving frame is in the open position, the first bearing position is located directly below the cap pressure post; at least two cap clamping blocks are disposed on the base at circumferential intervals along the cap pressure post, the clamping portion of the cap clamping block is lower than the cap pressure post, the cap clamping block is movable relative to the cap pressure post in the radial direction along the cap pressure post, and the at least two cap clamping blocks have adjacent clamping positions and distant opening positions.

[0007] Furthermore, a cap-piercing needle extending radially along the cap pressure post is provided on one side of the cap clamp block facing the axis of the cap pressure post.

[0008] Furthermore, the opening mechanism also includes a first driving member, a second driving member, and a third driving member. The first driving member is driven to the base to drive the base to move in the vertical direction relative to the moving frame. The second driving member is driven to the base to drive the base to rotate about the axis of the cap pressure column. The third driving member is driven to the cap clamping blocks to drive at least two cap clamping blocks to move between a clamping position and an open position.

[0009] Furthermore, the movable frame includes: a top plate, with both the first and second bearing positions disposed on the top plate; a bottom plate located below the top plate; a guide rod extending vertically, with one of the top plate and the bottom plate fixedly connected to the guide rod, and the other of the top plate and the bottom plate movably sleeved on the guide rod along the extension direction of the guide rod; and a buffer spring sleeved on the guide rod, with both ends of the buffer spring contacting the bottom plate and the top plate respectively.

[0010] Furthermore, the dispensing component includes a sample arm, on which a dispensing needle is provided.

[0011] Furthermore, the dispensing needle includes a first dispensing needle and a second dispensing needle, and when the moving frame moves to the dispensing position, the first dispensing needle and the second dispensing needle correspond to the first bearing position and the second bearing position, respectively; and / or, the dispensing mechanism also includes a TIP loading mechanism for storing the TIP head.

[0012] Furthermore, the first bearing position and the second bearing position are spaced apart in the direction of movement parallel to the moving frame; and / or, the transmission mechanism also includes a base on which the moving frame is movably mounted.

[0013] Furthermore, the transfer component includes a first guide rail and a gripper. The first guide rail extends vertically, and the gripper is movably disposed on the first guide rail along the extension direction of the first guide rail. The gripper includes at least two second grippers, and the detection component includes an optical fiber sensor disposed on the second grippers. The transfer component also includes a second guide rail and a third guide rail extending laterally. The second guide rail and the third guide rail are perpendicular to each other. The first guide rail is movably disposed on the second guide rail along the extension direction of the second guide rail, and the second guide rail is movably disposed on the third guide rail along the extension direction of the third guide rail.

[0014] Furthermore, the storage tube includes sample tubes and quality control tubes, and the storage compartment includes a sample storage compartment for storing sample tubes and a quality control product storage compartment for storing quality control tubes; the sample storage compartment and the quality control product storage compartment are set up independently; the quality control product storage compartment and the capping mechanism are located on both sides of the sample storage compartment, and the dispensing mechanism is located on the side of the capping mechanism away from the sample storage compartment.

[0015] According to another aspect of the present invention, a nucleic acid detection integrated machine is provided, comprising a reagent preparation device, a analyte extraction device, and an amplification detection device. The reagent preparation device is used to prepare extraction reagents and amplification reagents. The analyte extraction device is used to extract analytes from samples and combine the analytes with amplification reagents to form a detection mixture. The amplification detection device is used to amplify and detect the detection mixture. The analyte extraction device includes the analyte loading device provided above.

[0016] According to the technical solution of this invention, the test substance loading device includes a storage mechanism, a transfer mechanism, a transmission mechanism, a capping mechanism, and a filling mechanism. The storage tubes are mainly divided into two types: the first type has a cap, and the second type does not. When the moving frame moves to the loading position, the transfer component transfers the storage tube in the storage chamber to the moving frame. Before transfer, a detection component checks whether the storage tube to be transferred has a cap. If the storage tube to be transferred is found to have a cap, it is transferred to the first bearing position, while the second bearing position is empty. Then, the moving frame moves to the capping position, and the capping component opens the cap on the storage tube at the first bearing position. Then, it moves to the filling position, and the filling component fills the test substance in the storage tube into the reaction vessel. If the storage tube to be transferred is found to not have a cap, it is transferred to the first bearing position and / or the second bearing position, so that at least one of the first and second bearing positions has a storage tube. Then, the moving frame moves directly to the filling position, and the filling component fills the test substance in the storage tube into the reaction vessel. Therefore, when two types of storage tubes are used together, the detection device checks whether the storage tube to be transferred has a cap. The loading method of the first and second bearing positions on the moving frame can be flexibly selected, so that storage tubes with caps and those without caps can share a set of storage mechanism, transfer mechanism, transmission mechanism, cap opening mechanism and filling mechanism. This achieves structural compactness, ensures the consistency of the experimental process, and for storage tubes without caps, the moving frame can use the first and second bearing positions for transfer at the same time and skip the cap opening step to improve transportation efficiency. Attached Figure Description

[0017] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0018] Figure 1 A schematic diagram of the structure of the test object loading device provided according to an embodiment of the present invention is shown;

[0019] Figure 2 A schematic diagram of the opening mechanism and the transfer mechanism of the test object loading device provided according to an embodiment of the present invention is shown;

[0020] Figure 3 A schematic diagram of the opening mechanism of the test object loading device provided according to an embodiment of the present invention is shown;

[0021] Figure 4 This diagram shows a structural schematic of the opening mechanism of the test object loading device provided according to an embodiment of the present invention from another perspective;

[0022] Figure 5 A schematic diagram of the opening component of the test object loading device provided according to an embodiment of the present invention is shown;

[0023] Figure 6 A bottom view of the opening component of the test object loading device provided according to an embodiment of the present invention is shown;

[0024] Figure 7 A schematic diagram of the transmission mechanism of the test object loading device provided according to an embodiment of the present invention is shown;

[0025] Figure 8 A side view of the moving frame and clamping assembly of the test object loading device provided according to an embodiment of the present invention is shown;

[0026] Figure 9 A schematic diagram of the transfer mechanism of the test object loading device provided according to an embodiment of the present invention is shown;

[0027] Figure 10 A top view of the transfer mechanism of the test object loading device provided according to an embodiment of the present invention is shown;

[0028] Figure 11 A bottom view of the transmission mechanism of the test object loading device provided according to an embodiment of the present invention is shown;

[0029] Figure 12 A schematic diagram of the structure of a nucleic acid testing integrated machine provided according to an embodiment of the present invention is shown.

[0030] The above figures include the following reference numerals:

[0031] 10. Storage mechanism; 11. Storage compartment; 111. Sample storage compartment; 112. Quality control product storage compartment;

[0032] 20. Transfer mechanism; 21. Transfer component; 211. First guide rail; 212. Gripper; 213. Second guide rail; 214. Third guide rail; 22. Detection component; 221. Fiber optic sensor;

[0033] 30. Transmission mechanism; 31. Moving frame; 311. First bearing position; 312. Second bearing position; 313. Connecting block; 314. Top plate; 315. Bottom plate; 316. Guide rod; 317. Buffer spring;

[0034] 32. Clamping assembly; 321. First clamping member; 3211. First spring; 3212. First clamping block; 322. Second clamping member; 3221. Second spring; 3222. Second clamping block; 323. First driving member; 324. Sliding seat; 33. Base; 331. First groove; 332. Second groove; 34. Second driving member;

[0035] 40. Opening mechanism; 41. Opening component; 411. Base; 4111. Guide groove; 412. Cap clamping column; 413. Cap clamping block; 42. First driving component; 43. Second driving component; 44. Third driving component;

[0036] 50. Injection mechanism; 51. TIP loading mechanism;

[0037] 61. Reagent preparation device; 62. Analyte extraction device; 621. Analyte loading device; 63. Amplification and detection device. Detailed Implementation

[0038] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present invention or its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0039] like Figures 1 to 11As shown, this embodiment of the invention provides a test object loading device, which includes a storage mechanism 10, a transfer mechanism 20, a transmission mechanism 30, a cap-opening mechanism 40, and a filling mechanism 50. The storage mechanism 10 includes a storage compartment 11 for storing storage tubes. The transfer mechanism 20 includes a transfer component 21 and a detection component 22 disposed on the transfer component 21. The transmission mechanism 30 includes a movably disposed moving frame 31. The moving frame 31 has a first bearing position 311 and a second bearing position 312 for placing storage tubes, which are spaced apart. The moving frame 31 has a loading position, a cap-opening position, and a filling position. The moving frame 31 can move between the loading position, the cap-opening position, and the filling position. The moving frame 31 is positioned to load the storage tube into the first bearing position 311 and / or the second bearing position 312 when it moves to the loading position. The transfer member 21 can transfer the storage tube between the moving frame 31 and the storage chamber 11 to load the storage tube into the first bearing position 311 and / or the second bearing position 312. The opening mechanism 40 includes an opening member 41 that can open and close the storage tube. When the moving frame 31 moves to the opening position, the first bearing position 311 of the moving frame 31 corresponds to the opening member 41. The filling mechanism 50 includes a filling member that can fill the storage material in the storage tube into the reaction vessel. When the moving frame 31 moves to the filling position, the moving frame 31 is positioned to load the storage material into the reaction vessel.

[0040] The test substance loading device provided in this embodiment includes a storage mechanism 10, a transfer mechanism 20, a transmission mechanism 30, a capping mechanism 40, and a filling mechanism 50. The storage tubes are mainly divided into two types: the first type has a cap, and the second type does not. When the moving frame 31 moves to the loading position, the transfer component 21 transfers the storage tube in the storage chamber 11 to the moving frame 31. Before transfer, the detection component 22 detects whether the storage tube to be transferred has a cap. If the storage tube to be transferred is found to have a cap, it is transferred to the first bearing position 311, while the second bearing position 312 is empty. Then, the moving frame 31 moves to the capping position, and the capping component 41 opens the cap of the storage tube on the first bearing position 311. Finally, it moves to the filling position, and the filling component fills the test substance in the storage tube into the reaction vessel. If the storage tube to be transferred is found to be without a cap, it is transferred to the first bearing position 311 and / or the second bearing position 312, so that at least one of the first bearing position 311 and the second bearing position 312 is occupied by the storage tube. Then, the moving frame 31 moves directly to the filling position, and the test substance in the storage tube is filled into the reaction vessel using the filling device. Thus, in the case of mixing two types of storage tubes, the detection device 22 detects whether the storage tube to be transferred has a cap, and the loading method of the first bearing position 311 and the second bearing position 312 on the moving frame 31 is flexibly selected. This allows storage tubes with caps and those without caps to share a set of storage mechanism 10, transfer mechanism 20, transmission mechanism 30, capping mechanism 40, and filling mechanism 50, achieving structural compactness, ensuring the consistency of the experimental process, and for storage tubes without caps, the moving frame 31 can simultaneously use the first bearing position 311 and the second bearing position 312 for transfer, skipping the capping step, thereby improving transportation efficiency.

[0041] The movable frame 31 has the following three working states:

[0042] (1) The first bearing position 311 is used to place a storage tube with a tube cap, and the second bearing position is left empty;

[0043] (2) Both the first bearing position 311 and the second bearing position 312 are used to place storage tubes without tube caps;

[0044] (3) One of the first bearing position 311 and the second bearing position 312 is used to place a storage tube without a cap, and the other is left empty.

[0045] It should be noted that when the movable frame 31 moves to the open position, the first bearing position 311 of the movable frame 31 corresponds to the opening member 41. This means that, compared to the movable frame 31 in the filling position or the loading position, the distance between the movable frame 31 in the open position and the opening member 41 is smaller, thus enabling the opening member 41 to open the storage tube on the movable frame 31 in the open position. When the movable frame 31 moves to the filling position, the movable frame 31 is located in a position corresponding to the filling mechanism 50. This means that, compared to the movable frame 31 in the open position or the loading position, the distance between the movable frame 31 in the filling position and the opening member 41 is smaller, thus enabling the opening member 41 to be used to open the storage tube on the movable frame 31 in the open position. The movable frame 31 is positioned such that the distance between the movable frame 31 in the filling position and the filling mechanism 50 is smaller, thereby enabling the filling component to pick up the test sample from the storage tube on the movable frame 31 in the filling position; when the movable frame 31 moves to the loading position, the movable frame 31 is located in a position corresponding to the storage mechanism 10, meaning that the distance between the movable frame 31 in the loading position and the storage compartment 11 is smaller than that between the movable frame 31 in the filling position or the movable frame 31 in the open position, thereby enabling the transfer component 21 to transfer the storage tube between the storage compartment 11 and the movable frame 31 in the loading position.

[0046] like Figure 7 As shown, the transmission mechanism 30 also includes a clamping assembly 32, which includes a first clamping member 321 and a second clamping member 322 for clamping the storage tube. The first clamping member 321 is disposed on the movable frame 31 corresponding to the first bearing position 311, and the second clamping member 322 is disposed on the movable frame 31 corresponding to the second bearing position 312. During the transportation of the storage tube by the movable frame 31, the storage tube is clamped by the clamping assembly 32 by the first clamping member 321 and the second clamping member 322 respectively disposed around the first bearing position 311 and the second bearing position 312, thus preventing the storage tube from shaking during transportation.

[0047] like Figure 7As shown, the clamping assembly 32 also includes a first driving member 323 and a sliding seat 324. The sliding seat 324 is disposed on the movable frame 31 and located on the same side of the first bearing position 311 and the second bearing position 312. The first driving member 323 and the sliding seat 324 are drivenly connected to drive the sliding seat 324 to move relative to the first bearing position 311 and the second bearing position 312. The first clamping member 321 includes a first spring 3211 and a first clamping block 3212. The first clamping block 3212 is located on the side of the first bearing position 311 near the sliding seat 324. The two ends of the first spring 3211 are respectively connected to the first clamping block 3212 and the sliding seat 324. The second clamping member 322 includes a second spring 3221 and a second clamping block 3222. The second clamping block 3222 is located on the side of the second bearing position 312 near the sliding seat 324. The two ends of the second spring 3221 are respectively connected to the second clamping block 3222 and the sliding seat 324. The first driving member 323 drives the sliding seat 324 to move relative to the first bearing position 311 and the second bearing position 312, so that the moving frame 31 drives the first clamping block 3212 to clamp the storage tube on the first bearing position 311 through the first spring 3211, and the moving frame 31 drives the second clamping block 3222 to clamp the storage tube on the second bearing position 312 through the second spring 3221, so that the first clamping member 321 and the second clamping member 322 clamp synchronously, and the first spring 3211 and the second spring 3221 can play a buffering role, so that the first clamping member 321 and the second clamping member 322 are flexibly clamped.

[0048] like Figures 3 to 6 As shown, the cover opening component 41 includes a base 411, a cap pressing column 412, and at least two cap clamping blocks 413. The cap pressing column 412 is disposed on the base 411 and protrudes from the lower surface of the base 411. When the moving frame 31 is in the open position, the first bearing position 311 is located directly below the cap pressing column 412. At least two cap clamping blocks 413 are disposed on the base 411 at intervals along the circumference of the cap pressing column 412. The clamping part of the cap clamping block 413 is lower than the cap pressing column 412. The cap clamping block 413 can move relative to the cap pressing column 412 in the radial direction. At least two cap clamping blocks 413 have close clamping positions and far open positions. When the movable frame 31 is in the open position, the cap of the storage tube is pressed on the first bearing position 311 by the cap pressure column 412 to prevent the cap from tilting during the opening and closing process, which would affect the opening and closing effect. At least two cap clamping blocks 413 located in the clamping position are used to clamp the cap, which makes it easier to remove the cap from the storage tube later.

[0049] In this embodiment, a cap-piercing needle extending radially along the cap-pressing post 412 is provided on the side of the cap clamping block 413 facing the axis of the cap pressing post 412. When at least two cap clamping blocks 413 are in the clamping position, the cap-piercing needle pierces the cap, preventing relative sliding between the cap clamping block 413 and the cap during the opening process, which facilitates the removal of the cap from the storage tube.

[0050] like Figures 3 to 5 As shown, the cap-opening mechanism 40 also includes a first driving member 42, a second driving member 43, and a third driving member 44. The first driving member 42 is driven to the base 411 to drive the base 411 to move vertically relative to the moving frame 31. The second driving member 43 is driven to the base 411 to drive the base 411 to rotate around the axis of the cap pressing column 412. The third driving member 44 is driven to the cap clamping blocks 413 to drive at least two cap clamping blocks 413 to move between a clamping position and an open position. The first driving member 42 drives the base 411 to move downward relative to the moving frame 31, so that the cap pressing column 412 presses against the cap of the storage tube on the first bearing position 311. The third driving member 44 drives at least two cap clamping blocks 413 to move to the clamping position. Then, the first driving member 42 and the second driving member 43 drive the base 411 to rise and rotate relative to the moving frame 31, completing the cap-opening operation.

[0051] Specifically, for the cap that mates with the threaded part of the storage tube, when the third drive member 44 drives at least two cap clamping blocks 413 to move to the clamping position, the second drive member 43 first drives the base 411 to rotate relative to the moving frame 31. After the cap is completely unscrewed, the first drive member 42 drives the base 411 to move upward relative to the moving frame 31 to complete the cap opening operation. For the rubber cap fitted on the body of the storage tube, when the third drive member 44 drives at least two cap clamping blocks 413 to move to the clamping position, the first drive member 42 and the second drive member 43 simultaneously drive the base 411 to rise and rotate relative to the moving frame 31 to complete the cap opening operation.

[0052] like Figure 5 and Figure 6 As shown, a guide groove 4111 is provided on the base, and the cap clamp 413 can move in the guide groove 4111 along the extension direction of the guide groove 4111.

[0053] like Figure 8As shown, the movable frame 31 includes a top plate 314, a bottom plate 315, a guide rod 316, and a buffer spring 317. The first bearing position 311 and the second bearing position 312 are both set on the top plate 314. The bottom plate 315 is located below the top plate 314. The guide rod 316 extends vertically. One of the top plate 314 and the bottom plate 315 is fixedly connected to the guide rod 316. The other of the top plate 314 and the bottom plate 315 is movably sleeved on the guide rod 316 along the extension direction of the guide rod 316. The buffer spring 317 is sleeved on the guide rod 316. The two ends of the buffer spring 317 are in contact with the bottom plate 315 and the top plate 314, respectively. When the opening component 41 rotates to open the storage tube on the first bearing position 311, the up-and-down movement of the top plate 314 relative to the bottom plate 315 causes the buffer spring 317 to undergo elastic deformation. Under the action of the elastic force of the buffer spring 317, the first bearing position 311 and the second bearing position 312 are supported to float with the top plate 314 relative to the bottom plate 315, thereby simplifying the action of the opening component 41.

[0054] In this embodiment, the dispensing component includes a sample arm with a dispensing needle. When the moving frame 31 moves to the dispensing position, the sample arm drives the TIP head on the dispensing needle to move above the moving frame 31. The TIP head draws up the analyte from the storage tube, and the movement of the sample arm drives the TIP head to move above the reaction vessel, injecting the analyte from the TIP head into the corresponding position in the reaction vessel, thus improving the flexibility of the analyte loading device.

[0055] The sampling needle includes a first sampling needle and a second sampling needle. When the first and second sampling needles need to be used together to complete the injection action, there are three implementation methods:

[0056] (1) The distance between the first and second sampling needles is not adjustable. At this time, the distance between the first support position 311 and the second support position 312 is the same as the distance between adjacent or spaced reaction positions on the reaction vessel. Using the TIP head on the first and second sampling needles, the analyte in the storage tubes on the first support position 311 and the second support position 312 is simultaneously aspirated, and the aspirated analyte is simultaneously injected into the two adjacent or spaced reaction positions in the reaction vessel.

[0057] (2) The distance between the first sampling needle and the second sampling needle is not adjustable. At this time, the distance between the first support position 311 and the second support position 312 is different from the distance between the adjacent or spaced reaction positions on the reaction vessel. Using the TIP head on the first sampling needle and the TIP head on the second sampling needle, the analyte in the storage tube on the first support position 311 and the second support position 312 is sequentially aspirated, and the aspirated analyte is sequentially injected into the two adjacent or spaced reaction positions in the reaction vessel. If the distance between the first sampling needle and the second sampling needle is the same as the distance between the first support position 311 and the second support position 312 or the distance between the adjacent or spaced reaction positions on the reaction vessel, the first sampling needle and the second sampling needle can perform the action of aspirating or injecting the analyte simultaneously.

[0058] (3) The distance between the first and second sampling needles is adjustable. At this time, the distance between the first support position 311 and the second support position 312 is different from the distance between adjacent or spaced reaction positions on the reaction vessel. First, adjust the distance between the first and second sampling needles to the distance between the first support position 311 and the second support position 312. Use the TIP head on the first and second sampling needles to simultaneously aspirate the analyte in the storage tubes on the first support position 311 and the second support position 312. Then, adjust the distance between the first and second sampling needles to the distance between adjacent reaction positions on the reaction vessel. Use the TIP head on the first and second sampling needles to simultaneously inject the analyte into the two adjacent or spaced reaction positions in the reaction vessel.

[0059] In this embodiment, the sampling needle includes a first sampling needle and a second sampling needle. When the moving frame 31 moves to the injection position, the first sampling needle and the second sampling needle correspond to the first support position 311 and the second support position 312, respectively. Depending on the load-bearing conditions of the first support position 311 and the second support position 312, a tip is selected to be installed on the first sampling needle and / or the second sampling needle. When storage tubes are placed on both the first support position 311 and the second support position 312, by installing tip-heads on both the first and second sampling needles, the analyte in the storage tubes on the first support position 311 and the second support position 312 can be simultaneously aspirated and injected into the corresponding positions in the reaction vessel, thereby improving the efficiency of the analyte loading device.

[0060] like Figure 1As shown, the dispensing mechanism 50 also includes a TIP loading mechanism 51 for storing TIP heads. After the sample is injected into the reaction container using the TIP head, the TIP head on the sample arm is unloaded, and then the sample arm is moved to the TIP loading mechanism 51 to install a new TIP head on the sample arm. This allows the TIP head to be replaced for single use, avoiding cross-contamination between different samples and ensuring the accuracy of nucleic acid testing.

[0061] Specifically, by inserting the sampling needle into the opening of the TIP head in the TIP loading mechanism 51, an interference fit is used to make the TIP head fit onto the sampling needle.

[0062] like Figure 7 As shown, the first bearing position 311 and the second bearing position 312 are spaced apart in the moving direction parallel to the moving frame 31. During the movement of the moving frame 31, the first bearing position 311 and the second bearing position 312 are always moved synchronously, ensuring that the first bearing position 311 and the second bearing position 312 can pass under the cover member one after the other, making the structure of the transmission mechanism 30 more compact.

[0063] Furthermore, in the working state where a storage tube without a cap is placed in one of the first bearing position 311 and the second bearing position 312, and the other is empty, it is preferable that the storage tube without a cap is placed in the first bearing position 311 and the second bearing position is empty. On the one hand, this simplifies the judgment procedure, and on the other hand, since the first bearing position 311 and the second bearing position 312 are spaced apart in the moving direction parallel to the moving frame 31, the transportation path of the moving frame 31 can be shortened.

[0064] like Figure 1 As shown, the transmission mechanism 30 also includes a base 33, and a movable frame 31 is movably mounted on the base 33. The movement of the movable frame 31 on the base 33 makes its movement more stable, preventing the storage tube from falling during transport and ensuring the normal operation of the test object loading device.

[0065] like Figure 10 and Figure 11 As shown, the transmission mechanism also includes a second driving member 34. A connecting block 313 is located below the moving frame 31. A first groove 331 and a second groove 332 are arranged in parallel on the base 33. The second driving member 34 and the connecting block 313 are drivenly connected. The connecting block 313 is located in the first groove 331 and slides along the extension direction of the first groove 331. The first driving member 323 extends into the second groove 332 and slides along the extension direction of the second groove 332 as the moving frame 31 moves.

[0066] like Figure 9As shown, the transfer component 21 includes a first guide rail 211 and a gripper 212. The first guide rail 211 extends vertically, and the gripper 212 is movably mounted on the first guide rail 211 along its extension direction. The gripper 212 includes at least two second grippers. The detection component 22 includes an optical fiber sensor 221 mounted on the second grippers. When the gripper 212 is positioned above the storage tube to be gripped, causing it to move downwards along the first guide rail 211, the optical fiber sensor 221 detects the light transmittance of the storage tube. If the light transmittance of the storage tube changes significantly, it indicates that the storage tube has a cap; if the light transmittance of the storage tube does not change significantly, it indicates that the storage tube does not have a cap.

[0067] like Figure 9 As shown, the transfer component 21 also includes a second guide rail 213 and a third guide rail 214 extending laterally. The second guide rail 213 and the third guide rail 214 are perpendicular to each other. The first guide rail 211 is movably mounted on the second guide rail 213 along its extension direction, and the second guide rail 213 is movably mounted on the third guide rail 214 along its extension direction. Utilizing the first guide rail 211, the second guide rail 213, and the third guide rail 214, the gripper 212 can achieve movement in three directions, making the movement of the gripper 212 flexible and reliable.

[0068] like Figure 1 As shown, the storage tube includes sample tubes and quality control tubes. The storage compartment 11 includes a sample storage compartment 111 for storing sample tubes and a quality control product storage compartment 112 for storing quality control tubes. By storing the quality control products in the quality control tubes of the quality control product storage compartment 112, the extraction of quality control products and the collection of samples share a single dispensing mechanism 50, a transfer mechanism 30, and a transport mechanism 20, thereby improving the automation level of quality control product loading, simplifying the operation procedure for quality control product testing, and shortening the time required for quality control product testing.

[0069] like Figure 1 As shown, the sample storage chamber 111 and the quality control storage chamber 112 are set up independently. This independent setting prevents contamination between the sample tubes in sample storage chamber 111 and the quality control tubes in quality control storage chamber 112, ensuring the accuracy of nucleic acid testing and improving detection precision.

[0070] like Figure 1As shown, the quality control storage compartment 112 and the capping mechanism 40 are located on opposite sides of the sample storage compartment 111, and the dispensing mechanism 50 is located on the side of the capping mechanism 40 furthest from the sample storage compartment 111. With this spatial layout, since the dispensing mechanism 50 is located on the side of the capping mechanism 40 furthest from the sample storage compartment 111, the sample tubes and quality control tubes can pass through the capping mechanism 40 during transport between the storage mechanism 10 and the dispensing mechanism 50. This allows the capping mechanism 40 to perform opening and closing operations on the sample tubes and quality control tubes, shortening the moving distance of the moving frame 31. Under the premise that the moving speed of the moving frame 31 is constant, the moving time of the moving frame 31 can be shortened, reducing the time required for sample and quality control extraction. Furthermore, because the quality control storage compartment... 112 and the capping mechanism 40 are located on both sides of the sample storage chamber 111. Compared with the distance between the quality control storage chamber 112 and the capping mechanism 40, the distance between the sample storage chamber 111 and the capping mechanism 40 is shorter. In each batch of nucleic acid testing, the number of times the sample in the sample tube is extracted is much greater than the number of times the quality control material in the quality control tube is extracted. Therefore, compared with shortening the extraction time of the quality control material, shortening the extraction time of the sample can shorten the time consumed in each batch of nucleic acid testing to a greater extent and improve the working efficiency of the test material loading device.

[0071] like Figures 1 to 12 As shown, this embodiment of the invention provides a nucleic acid detection integrated machine, which includes a reagent preparation device 61, a analyte extraction device 62, and an amplification detection device 63. The reagent preparation device 61 is used to prepare extraction reagents and amplification reagents. The analyte extraction device 62 is used to extract analytes from samples and combine the analytes with amplification reagents to form a detection mixture. The amplification detection device 63 is used to amplify and detect the detection mixture. The analyte extraction device 62 includes the analyte loading device provided above. Using the nucleic acid detection integrated machine provided in this embodiment, the analyte loading device 621 can also detect whether the storage tube to be transported has a cap when two types of storage tubes are used together. The loading status of the first bearing position 311 and the second bearing position 312 on the moving frame 31 can be flexibly selected. When dealing with storage tubes without caps, the moving frame 31 can simultaneously utilize the first bearing position 311 and the second bearing position 312, skipping the capping step, thereby improving transportation efficiency.

[0072] Specifically, extraction reagents and amplification reagents are prepared by reagent preparation device 61, and the extraction reagents and amplification reagents are transported to analyte extraction device 62 respectively. Analyte extraction device 62 mixes the sample with the extraction reagents for extraction, and then combines the analyte with the amplification reagent to form a test mixture. The test mixture is transported to amplification detection device 63. After amplifying the analyte in the test mixture, the test mixture is analyzed and detected to obtain the test results of the sample.

[0073] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0074] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0075] In the description of this invention, it should be understood that the orientation or positional relationship indicated by directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" is generally based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this invention and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this invention; the directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.

[0076] For ease of description, spatial relative terms such as "above," "on top of," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "on top of" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0077] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore should not be construed as limiting the scope of protection of this invention.

[0078] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A device for loading an object to be tested, characterized in that, The test object loading device includes: Storage mechanism (10), including storage compartment (11) for storing storage tubes; The transfer mechanism (20) includes a transfer component (21) and a detection component (22) disposed on the transfer component (21); The transmission mechanism (30) includes a movable frame (31) that is movably arranged. The movable frame (31) is provided with a first bearing position (311) and a second bearing position (312) for placing the storage tube. The movable frame (31) has a loading position, an opening position and a filling position. The movable frame (31) can move between the loading position, the opening position and the filling position. When the movable frame (31) moves to the loading position, the movable frame (31) is located at a position corresponding to the storage mechanism (10). The transfer component (21) can transfer the storage tube between the movable frame (31) and the storage compartment (11) to load the storage tube onto the first bearing position (311) and / or the second bearing position (312). The opening mechanism (40) includes an opening member (41) capable of opening and closing the storage tube. When the moving frame (31) moves to the opening position, the first bearing position (311) of the moving frame (31) corresponds to the opening member (41). The dispensing mechanism (50) includes a dispensing element capable of dispensing the contents of the storage tube into the reaction vessel. When the movable frame (31) moves to the dispensing position, the movable frame (31) is located at a position corresponding to the dispensing mechanism (50).

2. The test object loading device according to claim 1, characterized in that, The cover opening component (41) includes: Base (411); The cap pressure column (412) is disposed on the base (411) and protrudes from the lower surface of the base (411). When the movable frame (31) is in the open position, the first bearing position (311) is located directly below the cap pressure column (412). At least two cap clamping blocks (413) are arranged circumferentially on the base (411) along the cap pressure post (412). The clamping portion of the cap clamping block (413) is lower than the cap pressure post (412). The cap clamping block (413) is movable relative to the cap pressure post (412) along the radial direction of the cap pressure post (412). At least two of the cap clamping blocks (413) have close clamping positions and far open positions.

3. The test object loading device according to claim 2, characterized in that, The cap clamp block (413) has a cap-piercing needle extending radially along the cap pressure post (412) on one side facing the axis of the cap pressure post (412).

4. The test object loading device according to claim 2, characterized in that, The opening mechanism (40) further includes a first driving member (42), a second driving member (43), and a third driving member (44). The first driving member (42) is driven to connect with the base (411) to drive the base (411) to move in the vertical direction relative to the moving frame (31). The second driving member (43) is driven to connect with the base (411) to drive the base (411) to rotate about the axis of the cap pressure column (412). The third driving member (44) is driven to connect with the cap clamping block (413) to drive at least two of the cap clamping blocks (413) to move between the clamping position and the opening position.

5. The test object loading device according to any one of claims 1 to 4, characterized in that, The movable frame (31) includes: The top plate (314) has the first bearing position (311) and the second bearing position (312) both disposed on the top plate (314); The bottom plate (315) is located below the top plate (314); A guide rod (316) extends vertically, and one of the top plate (314) and the bottom plate (315) is fixedly connected to the guide rod (316), while the other of the top plate (314) and the bottom plate (315) is movably sleeved on the guide rod (316) along the extension direction of the guide rod (316). A buffer spring (317) is sleeved on the guide rod (316), and the two ends of the buffer spring (317) are in contact with the bottom plate (315) and the top plate (314) respectively.

6. The test object loading device according to any one of claims 1 to 4, characterized in that, The dispensing device includes a sample arm, on which a dispensing needle is provided.

7. The test object loading device according to claim 6, characterized in that, The sampling needle includes a first sampling needle and a second sampling needle. When the moving frame (31) moves to the dispensing position, the first sampling needle and the second sampling needle correspond to the first bearing position (311) and the second bearing position (312) respectively; and / or, The dispensing mechanism (50) also includes a TIP loading mechanism (51) for storing the TIP header.

8. The test object loading device according to any one of claims 1 to 4, characterized in that, The first bearing position (311) and the second bearing position (312) are spaced apart in a direction parallel to the movement of the movable frame (31); and / or, The transmission mechanism (30) also includes a base (33), and the movable frame (31) is movably mounted on the base (33).

9. The test object loading device according to any one of claims 1 to 4, characterized in that, The transfer component (21) includes a first guide rail (211) and a gripper (212). The first guide rail (211) extends vertically, and the gripper (212) is movably disposed on the first guide rail (211) along the extension direction of the first guide rail (211). The gripper (212) includes at least two second grippers, and the detection component (22) includes an optical fiber sensor (221) disposed on the second grippers. The transfer component (21) further includes a second guide rail (213) and a third guide rail (214) extending laterally. The second guide rail (213) and the third guide rail (214) are perpendicular to each other. The first guide rail (211) is movably disposed on the second guide rail (213) along the extension direction of the second guide rail (213), and the second guide rail (213) is movably disposed on the third guide rail (214) along the extension direction of the third guide rail (214).

10. The test object loading device according to any one of claims 1 to 4, characterized in that, The storage tube includes a sample tube and a quality control tube, and the storage compartment (11) includes a sample storage compartment (111) for storing the sample tube and a quality control product storage compartment (112) for storing the quality control tube; The sample storage compartment (111) and the quality control product storage compartment (112) are set up independently; The quality control sample storage compartment (112) and the cap opening mechanism (40) are located on opposite sides of the sample storage compartment (111), and the filling mechanism (50) is located on the side of the cap opening mechanism (40) away from the sample storage compartment (111).

11. A nucleic acid testing integrated machine, characterized in that, The nucleic acid detection integrated machine includes a reagent preparation device (61), a analyte extraction device (62), and an amplification detection device (63). The reagent preparation device (61) is used to prepare extraction reagents and amplification reagents. The analyte extraction device (62) is used to extract analytes from samples and combine the analytes with amplification reagents to form a detection mixture. The amplification detection device (63) is used to amplify and detect the detection mixture. The analyte extraction device (62) includes the analyte loading device according to any one of claims 1 to 10.

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