Sample processing device and nucleic acid detection all-in-one machine having the same

Abstract

This invention provides a analyte processing device and a nucleic acid detection integrated machine having the same. The analyte processing device includes: a frame with a sample processing platform; an extraction mechanism for extracting analytes; a gripping part movably disposed above the sample processing platform; a transfer component movably disposed on the sample processing platform, the transfer component including an extraction plate transfer component having a first transfer position; the gripping part having a first gripping position and a second gripping position; and a pipetting part movably disposed above the sample processing platform. The extraction plate transfer component also has a sample dispensing position, and when the extraction plate transfer component is located at the sample dispensing position, the pipetting part also has a first pipetting position. The solution of this application can solve the problem of the large size of nucleic acid detection integrated machines.

Description

Technical Field

[0001] This invention relates to the field of biological detection technology, and more specifically, to a analyte processing device and a nucleic acid detection integrated machine having the same. Background Technology

[0002] Nucleic acid testing is currently widely used in clinical diagnosis, agricultural monitoring, food safety, and other fields. Especially during large-scale infectious disease outbreaks, nucleic acid testing can serve as the gold standard for disease diagnosis. The most commonly used nucleic acid testing method is PCR. The general principle of PCR is that, under the catalysis of DNA polymerase, using the parental DNA strand as a template and specific primers as extension start points, a daughter DNA strand complementary to the parental DNA strand is replicated in vitro.

[0003] In related technologies, nucleic acid testing machines require multiple modules to work together to extract parent DNA. The modules use movable grippers to transfer consumables, so as to extract parent DNA from the sample using extraction reagents.

[0004] However, transferring consumables between different modules requires different grippers. Alternatively, the operating area of ​​the grippers could be made large enough. Both of these solutions require a lot of space, resulting in an increased equipment size. Summary of the Invention

[0005] This invention provides a test sample processing device and a nucleic acid detection integrated machine having the same, to solve the problem of large size of nucleic acid detection integrated machines in related technologies.

[0006] According to one aspect of the present invention, an analyte processing apparatus is provided, comprising: a frame having a sample processing platform; an extraction mechanism for extracting an analyte from the analyte; a gripping part movably disposed above the sample processing platform along a vertical and a first horizontal direction; a transfer member movably disposed on the sample processing platform along a second horizontal direction perpendicular to the first horizontal direction, the transfer member including an extraction plate transfer member having a first transfer position corresponding to being moved below the gripping part, the gripping part having a first gripping position above the extraction plate transfer member and a second gripping position above the extraction mechanism, wherein when the extraction plate transfer member is located at the first transfer position, the gripping part is movable between the first gripping position and the second gripping position to move the extraction plate between the extraction plate transfer member and the extraction mechanism; and a pipetting part movably disposed above the sample processing platform; wherein the extraction plate transfer member further has a sample dispensing position moving to a corresponding pipetting part, and when the extraction plate transfer member is located at the sample dispensing position, the pipetting part further has a first pipetting position moving above the extraction plate transfer member.

[0007] Furthermore, the transfer device also includes an amplification plate transfer device. Both the extraction plate transfer device and the amplification plate transfer device move along a second horizontal direction. The amplification plate transfer device has a nucleic acid addition position flush with the extraction plate transfer device. The pipetting part also has a second pipetting position corresponding to the amplification plate transfer device. When the extraction plate transfer device is in the sample addition position and the amplification plate transfer device is in the nucleic acid addition position, the pipetting part can switch between the first pipetting position and the second pipetting position.

[0008] Furthermore, the extraction mechanism includes an extraction member and a transfer member movably disposed through the extraction member along a second horizontal direction. The transfer member has a transfer position corresponding to the gripping part below the extraction member and an extraction position corresponding to the extraction member below the extraction member. When the extraction plate transfer member is in the first transfer position and the transfer member is in the transfer position, the gripping part can move the extraction plate between the extraction plate transfer member and the transfer member.

[0009] Furthermore, the analyte processing device also includes a sealing mechanism for encapsulating an amplification plate. The sealing mechanism includes a carrier and a hot-pressing member for encapsulating the amplification plate. The hot-pressing member is located above the carrier. The carrier is movably disposed on the sample processing platform along a second horizontal direction. The carrier has an amplification plate bearing position for placing the amplification plate. The amplification plate transfer member has a second transfer position located below the gripping part. The gripping part also has a third gripping position located above the carrier and a fourth gripping position located above the amplification plate transfer member. When the amplification plate transfer member is located at the second transfer position, the gripping part can move between the third gripping position and the fourth gripping position to move the amplification plate from the amplification plate transfer member to the amplification plate bearing position.

[0010] Furthermore, the carrier also has a cover support position for placing the cover. When the gripping part is in the third gripping position, the carrier has a separation position where the cover support position moves below the gripping part to allow the gripping part to grip the cover, a release position where the amplification plate support position moves below the gripping part to allow the gripping part to release the cover to the amplification plate, and a sealing position where the amplification plate support position moves below the thermopressor.

[0011] Furthermore, the sealing mechanism includes a sealing frame and a testing component. The sealing frame is set on the sample processing platform, and the hot pressing component and the testing component are both fixedly set on the sealing frame. The carrier component is movably set on the sealing frame along the second horizontal direction, and the testing component can detect the bearing status of the carrier component.

[0012] Furthermore, the cover bearing position has a first limiting protrusion that limits the cover, and / or the amplification plate bearing position has a second limiting protrusion that limits the amplification plate.

[0013] Furthermore, the analyte processing device also includes a consumable storage compartment for carrying the pipette tip, and the pipette part has a loading position that moves above the consumable storage compartment to load the pipette tip.

[0014] Furthermore, the pipetting unit includes a sample arm and a pipetting needle. The sample arm is horizontally movable and mounted on the frame above the sample processing platform, while the pipetting needle is vertically movable and mounted on the sample arm.

[0015] Furthermore, the frame also includes a first guide rail and a second guide rail located above the sample processing platform. The first guide rail extends along a first horizontal direction, and the second guide rail extends along a second horizontal direction. The first guide rail is movably mounted on the second guide rail along the extension direction of the second guide rail, and the sample arm is movably mounted on the first guide rail along the extension direction of the first guide rail.

[0016] Furthermore, the gripping unit includes a gripping frame and a gripper that is vertically movable on the gripping frame. The gripping frame is movably mounted on the frame in a first horizontal direction, and the gripper has a first gripping position and a second gripping position.

[0017] According to another aspect of the present invention, a nucleic acid detection integrated machine is provided, which includes a reagent preparation device, a analyte processing device, and an amplification detection device. The reagent preparation device is used for preparing extraction reagents and amplification reagents, the amplification detection device is used for performing analyte detection on the test mixture to analyze the analyte, and the analyte processing device is the analyte processing device provided above.

[0018] Furthermore, the reagent preparation device, the analyte processing device, and the amplification detection device are isolated from each other. The reagent preparation device and the amplification detection device are located on opposite sides of the analyte processing device. A first channel is provided between the reagent preparation device and the analyte processing device, and a second channel is provided between the analyte processing device and the amplification detection device. Both the first and second channels have closed and open states. And / or, the nucleic acid detection integrated machine also includes a transfer mechanism disposed between the reagent preparation device and the analyte processing device. The transfer mechanism can move the extraction plate for preparing extraction reagents, the amplification plate for preparing amplification reagents, and the cover to the analyte processing device through the first channel. The gripping part of the analyte processing device can move the extraction plate to the extraction plate transfer position of the analyte processing device and move the amplification plate to the amplification plate transfer position of the analyte processing device.

[0019] According to the technical solution of this invention, the analyte processing device includes a frame, an extraction mechanism, a gripping part, a transfer member, and a pipetting part. When extracting analytes from the analyte, the pipetting part picks up the analyte, and then the extraction plate transfer member is moved to the sample application position. The pipetting part is then moved to a first pipetting position to release the analyte into the extraction plate of the extraction plate transfer member. The extraction plate transfer member is then moved to a first transfer position, and the gripping part is moved to a first gripping position above the extraction plate transfer member to grip the extraction plate on the extraction plate transfer member. The gripping part is then moved to a second gripping position above the extraction mechanism to release the extraction plate onto the extraction mechanism, whereby the extraction mechanism extracts the analyte. Since the analyte is extracted by the gripping part moving vertically and in a first horizontal direction, and by the extraction plate transfer member moving in a second horizontal direction, the space occupied by the analyte processing device can be greatly reduced, thereby reducing the overall size of the analyte processing device. Attached Figure Description

[0020] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings: Figure 1 A schematic diagram of the structure of the test object processing device provided according to an embodiment of the present invention is shown; Figure 2 Another schematic diagram of the test object processing apparatus provided according to an embodiment of the present invention is shown; Figure 3 It shows Figure 1 A schematic diagram of the extraction mechanism in the middle; Figure 4 It shows Figure 1 A schematic diagram of the sealing mechanism in the middle; Figure 5 It shows Figure 1 A schematic diagram of the pipetting section in the image.

[0021] The above figures include the following reference numerals: 10. Frame; 11. Sample processing platform; 12. First guide rail; 13. Second guide rail; 20. Loading components for the object under test; 30. Collection mechanism; 31. Collection component; 32. Transfer component; 40. Grabbing section; 50. Transfer component; 53. Extraction plate transfer component; 54. Amplification plate transfer component; 60. Pipette; 61. Sample arm; 62. Pipette needle; 70. Sealing mechanism; 71. Hot pressing component; 72. Supporting component; 721. Cover support position; 722. Amplification board support position; 73. Sealing frame; 80. Consumables storage compartment. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present invention or its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] like Figures 1 to 5 As shown, this embodiment of the invention provides a analyte processing device, which includes: a frame 10, an extraction mechanism 30, a gripping part 40, a transfer member 50, and a pipetting part 60. The frame 10 has a sample processing platform 11; the extraction mechanism 30 is used to extract the analyte from the analyte; the gripping part 40 is movably disposed above the sample processing platform 11 along a vertical and a first horizontal direction; the transfer member 50 is movably disposed on the sample processing platform 11 along a second horizontal direction perpendicular to the first horizontal direction, and the transfer member 50 includes an extraction plate transfer member 53, which has a first transfer position corresponding to being moved below the gripping part 40. The gripping part 40 has a first gripping position above the extraction plate transfer member 53 and a second gripping position above the extraction mechanism 30. When the extraction plate transfer member 53 is in the first transfer position, the gripping part 40 can move between the first gripping position and the second gripping position to move the extraction plate between the extraction plate transfer member 53 and the extraction mechanism 30. The pipetting part 60 is movably disposed above the sample processing platform 11. The extraction plate transfer member 53 also has a sample loading position that moves to the corresponding pipetting part 60. When the extraction plate transfer member 53 is in the sample loading position, the pipetting part 60 also has a first pipetting position that moves to the top of the extraction plate transfer member 53.

[0024] According to the technical solution of this invention, the analyte processing device includes a frame 10, an extraction mechanism 30, a gripping part 40, a transfer member 50, and a pipetting part 60. When extracting the analyte from the analyte, the pipetting part 60 first picks up the analyte, then the extraction plate transfer member 53 is moved to the sample application position, and the pipetting part 60 is moved to the first pipetting position so that the pipetting part 60 releases the analyte into the extraction plate of the extraction plate transfer member 53. The extraction plate transfer member 53 is then moved to the first transfer position, and the gripping part 40 is moved to the first gripping position above the extraction plate transfer member 53 to grip the extraction plate on the extraction plate transfer member 53. The gripping part 40 is then moved to the second gripping position above the extraction mechanism 30 to release the extraction plate onto the extraction mechanism 30, and then the extraction mechanism 30 extracts the analyte from the analyte. Since the analyte is extracted by moving the gripping part 40 in the vertical and first horizontal directions and by moving the extraction plate transfer part 53 in the second horizontal direction, the space occupied by the analyte processing device can be greatly reduced, thereby reducing the volume of the analyte processing device.

[0025] It should be noted that the analyte can be transferred from outside the device to the sample processing platform 11 inside the device, or the analyte loading device 20 can be set on the sample processing platform 11 and the analyte can be placed in the analyte loading device 20.

[0026] In this embodiment, the test sample loading member 20 includes a test sample compartment for holding the test sample and a cap opening mechanism. The test sample is placed in a test sample tube, which is placed in the test sample compartment. The cap is opened by the cap opening mechanism, and the opened test sample tube is conveyed to the bottom of the pipetting section 60 to aspirate the test sample.

[0027] It should be noted that the analyte includes one or more of the following: the sample to be tested, the quality control sample, or the calibration sample.

[0028] like Figure 1 and Figure 3 As shown, the extraction mechanism 30 includes an extraction member 31 and a transfer member 32 movably disposed through the extraction member 31 along a second horizontal direction. The transfer member 32 has a transfer position corresponding to a position below the gripping part 40 and an extraction position corresponding to a position below the extraction member 31. When the extraction plate transfer member 53 is in the first transfer position and the transfer member 32 is in the transfer position, the gripping part 40 can move the extraction plate between the extraction plate transfer member 53 and the transfer member 32. With the above structure, the transfer member 32 can realize the transfer of the extraction plate between the extraction plate transfer member 53 and the extraction member 31, which has the advantages of simple structure and easy implementation.

[0029] In this embodiment, both the extraction plate transfer member 53 and the transfer member 32 can move along the second horizontal direction to below the gripping part 40. For example... Figures 1 to 3As shown, the transfer unit 50 includes an amplification plate transfer unit 54, an extraction plate transfer unit 53, and an amplification plate transfer unit 54, both of which move along a second horizontal direction. The amplification plate transfer unit 54 has a nucleic acid loading position flush with the extraction plate transfer unit 53. The pipetting unit 60 also has a second pipetting position corresponding to the amplification plate transfer unit 54. When the extraction plate transfer unit 53 is in the sample loading position and the amplification plate transfer unit 54 is in the nucleic acid loading position, the pipetting unit 60 can switch between the first pipetting position and the second pipetting position. When the amplification plate transfer unit 54 is in the nucleic acid loading position and the extraction plate transfer unit 53 is in the sample loading position, the pipetting unit 60 can be used to transfer the extracted nucleic acid from the extraction plate on the extraction plate transfer unit 53 to the amplification plate on the amplification plate transfer unit 54, which has the advantages of compact structure and easy operation.

[0030] In this embodiment, the extraction plate transfer member 53 and the amplification plate transfer member 54 are arranged in parallel. The sample processing platform 11 is provided with two parallel tracks, both of which extend along the second horizontal direction. The extraction plate transfer member 53 and the amplification plate transfer member 54 are respectively movably arranged on the corresponding tracks.

[0031] It should be noted that after the analyte in the extraction plate is extracted by the extraction mechanism 30, the extraction plate is gripped by the gripping part 40 and released onto the extraction plate transfer member 53 located at the first transfer position. The extraction plate transfer member 53 is then moved to the sample loading position, and the pipetting part 60 is moved to the first pipetting position to aspirate the extracted analyte from the extraction plate. Then, the pipetting part 60 is moved to the second pipetting position located above the amplification plate transfer member 54 to add the analyte into the amplification plate, thus completing the transfer of the analyte.

[0032] The amplification plate transfer member 54 also has a second transfer position that moves to a position below the gripping part 40 so that the gripping part 40 can load the amplification plate.

[0033] Since the extraction plate is transferred between the extraction plate transfer member 53 and the extraction mechanism 30 by moving the gripping part 40 along the vertical and first horizontal directions; and since the extraction plate transfer member 53, the amplification plate transfer member 54 and the transfer member 32 are all transferred along the second horizontal direction, it is possible to realize the extraction of the analyte by the pipetting part 60 and the transfer to the extraction member 31 for analyte extraction, and the transfer of the extracted analyte from the extraction plate to the amplification plate. This makes the arrangement of each component more reasonable and compact, thereby reducing the size of the device.

[0034] like Figure 1 and Figure 4As shown, the analyte processing device also includes a sealing mechanism 70 for encapsulating an amplification plate. The sealing mechanism 70 includes a carrier 72 and a hot-pressing member 71 for encapsulating the amplification plate. The hot-pressing member 71 is located above the carrier 72. The carrier 72 is movably disposed on the sample processing platform 11 along a second horizontal direction. The carrier 72 has an amplification plate bearing position 722 for placing the amplification plate. The amplification plate bearing position 722 has a third transfer position corresponding to being moved below the gripping part 40. The amplification plate transfer member 54 has a second transfer position located below the gripping part 40. The gripping part 40 also has a third gripping position located above the carrier 72 and a fourth gripping position located above the amplification plate transfer member 54. When the amplification plate transfer member 54 is located at the second transfer position and the amplification plate bearing position 722 is located at the third transfer position, the gripping part 40 can move between the third gripping position and the fourth gripping position to move the amplification plate from the amplification plate transfer member 54 to the amplification plate bearing position 722. With the above structure, the amplification plate can be supported by the carrier 72, and the amplification plate loaded with analytes can be moved onto the carrier 72 by the gripping part 40, and then the amplification plate can be packaged by the hot pressing part 71.

[0035] Furthermore, the transfer of the extraction plate and the amplification plate is performed using the gripping unit 40. During the transfer of the extraction plate and the amplification plate, the extraction plate and the amplification plate can share a common moving path, which helps to reduce space occupation and make the structure of the test analyte processing device more compact.

[0036] like Figure 4 As shown, the carrier 72 also has a cover support position 721 for placing the cover. When the gripping part 40 is in the third gripping position, the carrier 72 has a separation position where the cover support position 721 moves below the gripping part 40 to allow the gripping part 40 to grip the cover, a release position where the amplification plate support position 722 moves below the gripping part 40 to allow the gripping part 40 to release the cover to the amplification plate, and a sealing position where the amplification plate support position 722 moves below the thermoforming member 71. Within the range of motion of the gripping part 40, the cover and amplification plate can be sealed by the carrier 72 moving along the second horizontal direction in conjunction with the gripping part 40, which has the advantage of a compact structure.

[0037] Specifically, when the gripping part is in the fourth gripping position, the carrier 72 is moved to the separation position, allowing the gripping part 40 to grip the cover and detach it from the cover support position 721. The carrier 72 is then moved along the second horizontal direction to the release position, moving the amplification plate support position 722 below the gripping part 40 to release the cover onto the amplification plate. The carrier 72 is then moved to the encapsulation position, where the thermoforming member 71 encapsulates the cover and the amplification plate.

[0038] like Figure 4As shown, the sealing mechanism 70 includes a sealing frame 73 and a detection element. The sealing frame 73 is mounted on the sample processing platform 11. The heat-pressing element 71 and the detection element are both fixedly mounted on the sealing frame 73. The carrier element 72 is movably mounted on the sealing frame 73 along a second horizontal direction. The detection element can detect the bearing status of the carrier element 72. The detection element can detect whether an amplification plate, a cover, or a packaged amplification plate and a cover are mounted on the carrier element 72.

[0039] In this embodiment, the cover support position 721 has a first limiting protrusion that limits the cover, and the amplification plate support position 722 has a second limiting protrusion that limits the amplification plate. The first limiting protrusion can limit the cover, making the cover more stable on the cover support position 721. The second limiting protrusion can limit the amplification plate, making the amplification plate more stable on the amplification plate support position 722.

[0040] like Figure 2 As shown, the analyte processing device also includes a consumable storage compartment 80 for holding a pipette tip, and a loading position for the pipette tip to be moved above the consumable storage compartment 80. The consumable storage compartment 80 allows for the storage of the pipette tip, while the pipette tip can be loaded using the pipette section 60 and replaced before the pipette tip aspirates the analyte or analyte, thus preventing contamination between extraction reagents, amplification reagents, the analyte, and the analyte.

[0041] Specifically, before the pipette 60 aspirates the analyte from the test tube, the pipette 60 needs to be moved above the consumable storage compartment 80 to load the pipette tip. The analyte is then aspirated using the pipette tip. The process for aspirating the analyte is similar and will not be described in detail here.

[0042] like Figure 1 As shown, the pipetting unit 60 includes a sample arm 61 and a pipette 62. The sample arm 61 is horizontally movable and mounted on the frame 10, located above the sample processing platform 11. The pipette 62 is vertically movable and mounted on the sample arm 61. With the above structure, the pipetting unit 60 can achieve triaxial movement of the pipette 62 by cooperating with the sample arm 61 and the pipette 62.

[0043] In this embodiment, a pipette tip can be loaded using a pipette 62. Of course, multiple pipettes 62 can also be provided on the sample arm 61, and a different pipette 62 can be used for each operation to prevent contamination.

[0044] like Figure 1 and Figure 5As shown, the frame 10 also includes a first guide rail 12 and a second guide rail 13 located above the sample processing platform 11. The first guide rail 12 extends along a first horizontal direction, and the second guide rail 13 extends along a second horizontal direction. The first guide rail 12 is movably mounted on the second guide rail 13 along its extension direction, and the sample arm 61 is movably mounted on the first guide rail 12 along its extension direction. The use of the first guide rail 12 and the second guide rail 13 enables the sample arm 61 to move horizontally, offering the advantage of a simple structure.

[0045] like Figure 5 As shown, the gripping unit 40 includes a gripping frame and a gripper that is vertically movable on the gripping frame. The gripping frame is movably mounted on the frame 10 along a first horizontal direction, and the gripper has a first gripping position and a second gripping position. The gripping unit 40 with the above structure enables the gripper to move along two axes in the vertical plane using the gripping frame, and has the advantage of simple structure.

[0046] The following describes the usage process of the analyte processing device. When extracting the analyte from the analyte, the pipette 62 on the pipette section 60 is first used to aspirate the analyte. Then, the pipette 62 is moved to the first pipetting position above the extraction plate transfer member 53, and the extraction plate transfer member 53 is moved to the sample application position. The pipette 62 then releases the analyte into the extraction plate of the extraction plate transfer member 53. Next, the extraction plate transfer member 53 is moved to the first transfer position below the gripping section 40, and the gripping section 40 is moved to the first gripping position to grip the extraction plate on the extraction plate transfer member 53. Then, the gripping section 40 is moved to the second gripping position, placing it above the transfer member 32 in the transfer position, to release the extraction plate onto the transfer member 32. The transfer member 32 is then moved to the extraction position in the extraction member 31, whereby the analyte is extracted using the extraction member 31. After analyte extraction, the transporter 32 is moved from the extraction position to the transport position, and the gripping part 40 grips the extraction plate and releases it onto the extraction plate transfer member 53 located at the first transfer position. The extraction plate transfer member 53 is then moved to the sample loading position, and the pipette 62 is moved to the first pipetting position to aspirate the extracted analyte from the extraction plate. The pipette 62 is then moved to the second pipetting position, positioned above the amplification plate transfer member 54 at the nucleic acid loading position, to release the analyte into the amplification plate. The amplification plate transfer member 54 is then moved to the second transfer position, and the gripping unit 40 is moved to the fourth gripping position. The gripping unit 40 grips the amplification plate, and then moves to the third gripping position above the carrier member 72. The amplification plate is released to the amplification plate carrier position 722, and the carrier member 72 is moved to the separation position. The gripping unit 40 grips the cover, and then the carrier member 72 is moved to the release position to release the cover to the amplification plate. Finally, the carrier member 72 is moved to the encapsulation position, and the cover and amplification plate are encapsulated using the thermoforming member 71. Therefore, the analyte processing device can realize the transfer of the extraction plate, amplification plate, and cover along the same path, as well as the merging of the amplification plate and cover. It has the characteristics of simple structure and reduces the volume of the analyte processing device.

[0047] Another embodiment of the present invention provides a nucleic acid detection integrated machine, which includes a reagent preparation device, a analyte processing device, and an amplification detection device. The reagent preparation device is used for preparing extraction reagents and amplification reagents, the amplification detection device is used for analyzing and detecting the mixture, and the analyte processing device is the analyte processing device provided above. By using this nucleic acid detection integrated machine, the analyte is extracted by the gripping part 40 moving vertically and in a first horizontal direction, and by the transfer member 50 moving in a second horizontal direction. This significantly reduces the space occupied by the analyte processing device, thereby reducing its volume.

[0048] In this embodiment, the reagent preparation device, the analyte processing device, and the amplification detection device are isolated from each other. The reagent preparation device and the amplification detection device are located on opposite sides of the analyte processing device. A first channel is provided between the reagent preparation device and the analyte processing device, and a second channel is provided between the analyte processing device and the amplification detection device. Both the first and second channels have closed and open states. This structure allows for isolation between the individual device modules by utilizing the closed state of the first and second channels, preventing cross-contamination. The open state of the first and second channels enables the transfer of consumables between the device modules.

[0049] The nucleic acid testing integrated machine also includes a transfer mechanism disposed between the reagent preparation device and the analyte processing device. This transfer mechanism can move the extraction plate (containing the preparation of extraction reagents), the amplification plate (containing the preparation of amplification reagents), and the cover to the analyte processing device via a first channel. The gripping part 40 of the analyte processing device can move the extraction plate to the extraction plate transfer member 53 and the amplification plate to the amplification plate transfer member 54. Using this transfer mechanism, the extraction plate (containing the preparation of extraction reagents) and the amplification plate (containing the preparation of amplification reagents) in the reagent preparation device can be moved to the analyte processing device for analyte extraction.

[0050] In this embodiment, the extraction plate, amplification plate, and cover are all transferred from the reagent preparation device to the analyte processing device via a transfer mechanism. The gripping unit 40 can transfer the extraction plate, amplification plate, and cover separately. The extraction plate is placed on the extraction plate transfer member 53, the amplification plate is placed on the amplification plate transfer member 54, and the cover is placed on the cover bearing position 721 of the carrier member 72. The transfer mechanism is located within the movement range of the gripping unit 40. Therefore, the transfer paths of the gripping unit 40 in transferring the extraction plate from the transfer mechanism to the extraction plate transfer member, transferring the amplification plate from the transfer mechanism to the amplification plate transfer member, and transferring the cover from the transfer mechanism to the carrier member 72 can all overlap, resulting in a more compact structure.

[0051] Below the gripping part 40, along the moving direction of the gripping part 40, the running track of the ferry mechanism, the running track of the transfer component 50, the running track of the transfer component 32, and the running track of the carrier component 72 are arranged in sequence, making the structure more compact.

[0052] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0053] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps set forth in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0054] In the description of this invention, it should be understood that the orientation or positional relationship indicated by directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" is generally based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this invention and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this invention; the directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.

[0055] For ease of description, spatial relative terms such as "above," "on top of," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation beyond the orientation of the device as described in the figures. For example, if the device in the figures were inverted, a device described as "above" or "on top of" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0056] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore should not be construed as limiting the scope of protection of this invention.

[0057] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A device for processing a test object, characterized in that The test object processing device includes: The frame (10) has a sample processing platform (11). Extraction mechanism (30) is used to extract analytes from the analyte; The gripping part (40) is movably disposed above the sample processing platform (11) in both the vertical and first horizontal directions; A transfer member (50) is movably disposed on the sample processing platform (11) along a second horizontal direction perpendicular to the first horizontal direction. The transfer member (50) includes an extraction plate transfer member (53). The extraction plate transfer member (53) has a first transfer position corresponding to being moved below the gripping part (40). The gripping part (40) has a first gripping position above the extraction plate transfer member (53) and a second gripping position above the extraction mechanism (30). When the extraction plate transfer member (53) is located at the first transfer position, the gripping part (40) can move between the first gripping position and the second gripping position to move the extraction plate between the extraction plate transfer member (53) and the extraction mechanism (30). A pipetting section (60) is movably disposed above the sample processing platform (11); The extraction plate transfer member (53) also has a sample application position that can be moved to the corresponding pipetting part (60). When the extraction plate transfer member (53) is located at the sample application position, the pipetting part (60) also has a first pipetting position that can be moved to the top of the extraction plate transfer member (53).

2. The object under test handling device according to claim 1, characterized in that, The transfer unit (50) further includes an amplification plate transfer unit (54). Both the extraction plate transfer unit (53) and the amplification plate transfer unit (54) move along the second horizontal direction. The amplification plate transfer unit (54) has a nucleic acid addition position that is flush with the extraction plate transfer unit (53). The pipetting part (60) also has a second pipetting position corresponding to the amplification plate transfer unit (54). When the extraction plate transfer unit (53) is located at the sample addition position and the amplification plate transfer unit (54) is located at the nucleic acid addition position, the pipetting part (60) can switch between the first pipetting position and the second pipetting position.

3. The object under test handling device according to claim 2, characterized in that, The extraction mechanism (30) includes an extraction member (31) and a transfer member (32) movably disposed in the extraction member (31) along the second horizontal direction. The transfer member (32) has a transfer position corresponding to the gripping part (40) and an extraction position corresponding to the extraction member (31). When the extraction plate transfer member (53) is located in the first transfer position and the transfer member (32) is located in the transfer position, the gripping part (40) can move the extraction plate between the extraction plate transfer member (53) and the transfer member (32).

4. The test object processing device according to claim 2, characterized in that, The analyte processing device further includes a sealing mechanism (70) for encapsulating an amplification plate. The sealing mechanism (70) includes a carrier (72) and a hot press (71) for encapsulating the amplification plate. The hot press (71) is located above the carrier (72). The carrier (72) is movably disposed on the sample processing platform (11) along the second horizontal direction. The carrier (72) has an amplification plate carrier position (722) for placing the amplification plate. The amplification plate transfer member (54) has a second transfer position located below the gripping part (40). The gripping part (40) also has a third gripping position located above the carrier (72) and a fourth gripping position located above the amplification plate transfer member (54). When the amplification plate transfer member (54) is located at the second transfer position, the gripping part (40) can move between the third gripping position and the fourth gripping position to move the amplification plate from the amplification plate transfer member (54) to the amplification plate carrier position (722).

5. The test object processing device according to claim 4, characterized in that, The carrier (72) also has a cover support position (721) for placing the cover, and when the gripping part (40) is in the third gripping position, the carrier (72) has a separation position where the cover support position (721) moves below the gripping part (40) so that the gripping part (40) grips the cover, the separation position where the amplification plate support position (722) moves below the gripping part (40) so that the gripping part (40) releases the cover to the amplification plate, and a sealing position where the amplification plate support position (722) moves below the thermopressor (71).

6. The test object processing device according to claim 5, characterized in that, The sealing mechanism (70) includes a sealing frame (73) and a detection component. The sealing frame (73) is disposed on the sample processing platform (11). The hot pressing component (71) and the detection component are both fixedly disposed on the sealing frame (73). The carrier component (72) is movably disposed on the sealing frame (73) along the second horizontal direction. The detection component can detect the bearing state of the carrier component (72).

7. The test object processing device according to claim 5, characterized in that, The cover support position (721) has a first limiting protrusion that limits the cover, and / or the amplification plate support position (722) has a second limiting protrusion that limits the amplification plate.

8. The test object processing device according to claim 1, characterized in that, The test sample processing device also includes a consumable storage compartment (80) for carrying a pipette tip, and the pipette part (60) has a loading position that moves above the consumable storage compartment (80) to load the pipette tip.

9. The test object processing device according to claim 1, characterized in that, The pipetting unit (60) includes a sample arm (61) and a pipetting needle (62). The sample arm (61) is horizontally movable on the frame (10) and located above the sample processing platform (11). The pipetting needle (62) is vertically movable on the sample arm (61).

10. The test object processing device according to claim 9, characterized in that, The frame (10) also includes a first guide rail (12) and a second guide rail (13) located above the sample processing platform (11). The first guide rail (12) extends along the first horizontal direction, and the second guide rail (13) extends along the second horizontal direction. The first guide rail (12) is movably disposed on the second guide rail (13) along the extension direction of the second guide rail (13), and the sample arm (61) is movably disposed on the first guide rail (12) along the extension direction of the first guide rail (12).

11. The test object processing device according to claim 1, characterized in that, The gripping part (40) includes a gripping frame and a gripper that is vertically movable on the gripping frame. The gripping frame is movably mounted on the frame (10) along the first horizontal direction. The gripper has a first gripping position and a second gripping position.

12. A nucleic acid testing integrated machine, characterized in that, The nucleic acid detection integrated machine includes a reagent preparation device, a analyte processing device, and an amplification detection device. The reagent preparation device is used for preparing extraction reagents and amplification reagents. The amplification detection device is used for analyzing the analyte by performing analyte detection on the test mixture. The analyte processing device is the analyte processing device according to any one of claims 1 to 11.

13. The nucleic acid testing integrated machine according to claim 12, characterized in that, The reagent preparation device, the analyte processing device, and the amplification detection device are isolated from each other. The reagent preparation device and the amplification detection device are located on opposite sides of the analyte processing device. A first channel is provided between the reagent preparation device and the analyte processing device, and a second channel is provided between the analyte processing device and the amplification detection device. Both the first channel and the second channel have a closed state and a conductive state; and / or, The nucleic acid testing integrated machine also includes a transfer mechanism disposed between the reagent preparation device and the test sample processing device. The transfer mechanism can move the extraction plate for preparing extraction reagent, the amplification plate for preparing amplification reagent, and the cover to the test sample processing device through the first channel. The gripping part (40) of the test sample processing device can move the extraction plate to the extraction plate transfer part (53) of the test sample processing device and move the amplification plate to the amplification plate transfer part (54) of the test sample processing device.

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