Moisturizing and anti-wrinkle composition and preparation method thereof

Abstract

The present invention relates to a moisturizing and anti-wrinkle composition and a preparation method thereof, and belongs to the technical field of anti-wrinkle skin care products. The moisturizing and anti-wrinkle composition is prepared from raw materials including honeysuckle, rhus chinensis leaves, honeysuckle, and huoshan dendrobium, and the four have a synergistic effect, and the free radical scavenging ability of the prepared composition is significantly enhanced, that is, the antioxidant performance is improved and the moisturizing performance is enhanced; at the same time, the composition increases the water content of the skin, thereby achieving the effect of protecting the skin and delaying aging. Inula flower is also added to the raw materials of the present invention to prepare the moisturizing and anti-wrinkle composition, which produces a synergistic effect with honeysuckle, and significantly enhances the free radical scavenging performance of the moisturizing and anti-wrinkle composition.

Description

Technical Field

[0001] The invention belongs to the technical field of anti-wrinkle skin care products, and in particular, relates to a moisturizing anti-wrinkle composition and a preparation method thereof. Background Art

[0002] Skin is an important barrier of the human body. Wrinkles are the result of the combined effects of natural aging and photoaging of the skin. Natural aging is a natural process of metabolism that causes fine wrinkles on the skin surface. Photoaging is an accelerator of skin aging, which causes wrinkles to deepen. Free radicals can be produced when the skin is exposed to ultraviolet rays, causing oxidative damage to proteins and DNA, leading to damage to collagen and elastic fibers. Free radicals can also upregulate the expression of matrix metalloproteinases, which can destroy collagen and elastic fibers and accelerate wrinkle formation. In addition, the inertia of fibroblasts and the reduction of collagen synthesis are also one of the causes of wrinkles.

[0003] Skin moisturizing is the basis of skin care, and it plays an important role in maintaining the skin's luster and elasticity and enhancing its barrier properties. When the stratum corneum's own moisturizing factor decreases, the hydration capacity decreases, resulting in skin sagging, dryness, desquamation, fine wrinkles, etc. Taking effective moisturizing measures for the skin can increase the skin's water content, thereby protecting the skin and delaying aging. Summary of the invention

[0004] The present invention relates to a moisturizing and anti-wrinkle composition and a preparation method thereof, and belongs to the technical field of anti-wrinkle skin care products. The moisturizing and anti-wrinkle composition is prepared from raw materials including honeysuckle, rhus chinensis leaves, honeysuckle, and huoshan dendrobium, and the four have a synergistic effect, and the free radical scavenging ability of the prepared composition is significantly enhanced, that is, the antioxidant performance is improved and the moisturizing performance is enhanced; at the same time, the composition increases the water content of the skin, thereby achieving the effect of protecting the skin and delaying aging. Inula flower is also added to the raw materials of the present invention to prepare the moisturizing and anti-wrinkle composition, which produces a synergistic effect with honeysuckle, and significantly enhances the free radical scavenging performance of the moisturizing and anti-wrinkle composition.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A moisturizing and anti-wrinkle composition is prepared from the following raw materials in parts by weight:

[0007] 10-20 parts of honeysuckle phyllostachys, 15-30 parts of rhus chinensis leaves, 15-30 parts of honeysuckle, and 20-50 parts of Huoshan dendrobium.

[0008] As a preferred embodiment of the present invention, the raw materials of the moisturizing and anti-wrinkle composition further include 15 to 30 parts by weight of Inula fructus.

[0009] The preparation method of the above-mentioned moisturizing and anti-wrinkle composition comprises the following operations:

[0010] S1: taking raw materials of Lonicera japonica, Rhus chinensis leaves, honeysuckle and Dendrobium huoshanense to prepare Lonicera japonica extract, Rhus chinensis leaves extract, honeysuckle extract and Dendrobium huoshanense extract respectively;

[0011] S2: Mix the extract of Lonicera japonica, the extract of Rhus chinensis leaves, the extract of Honeysuckle and the extract of Dendrobium huoshanense to prepare a mixture, and dissolve the mixture in an ethanol aqueous solution with a volume fraction of 20-60% to prepare a moisturizing and anti-wrinkle composition.

[0012] As a preferred embodiment of the present invention, the volume ratio of the mixture to the ethanol aqueous solution in S2 is 1:4-6.

[0013] As a preferred embodiment of the present invention, the Lonicera japonica extract described in S1 is prepared by the following operations: crushing the raw materials Lonicera japonica and Dendrobium huoshanense, extracting them with an ethanol aqueous solution with a volume fraction of 90-95%, taking the solid after the extraction to obtain defatted medicinal residues, and precipitating the defatted medicinal residues with alcohol, centrifuging, and freeze-drying to obtain the Lonicera japonica extract and the Dendrobium huoshanense extract.

[0014] As a preferred embodiment of the present invention, the Rhus chinensis leaf extract and / or the honeysuckle extract in S1 are prepared by the following operations: crushing the raw Rhus chinensis leaves and / or honeysuckle flowers, extracting them with an ethanol aqueous solution with a volume fraction of 60-65%, then removing the solvent, drying to obtain a crude extract, separating the crude extract with a macroporous resin, eluting with an ethanol aqueous solution, concentrating and drying to obtain the Rhus chinensis leaf extract and / or honeysuckle extract.

[0015] As a preferred embodiment of the present invention, the material-liquid ratio of the Rhus chinensis leaves and / or honeysuckle raw materials to the ethanol aqueous solution in the extraction is 1 g: 12-18 mL.

[0016] As a preferred embodiment of the present invention, the Dendrobium huoshanense extract in S1 is prepared by the following operations:

[0017] The raw material Huoshan Dendrobium is crushed, then extracted with water, and concentrated under reduced pressure to obtain a Huoshan Dendrobium crude extract, which is extracted with an ethanol aqueous solution with a volume fraction of 90-98%, the precipitate is dried, and the protein is removed, and then extracted with an ethanol aqueous solution with a volume fraction of 90-98%, the precipitate is dried, and the Huoshan Dendrobium extract is obtained.

[0018] As a preferred embodiment of the present invention, S1 is replaced by: taking raw materials of Lonicera japonica, Rhus chinensis leaves, honeysuckle, Dendrobium huoshanense and Inula fructus to prepare Lonicera japonica extract, Rhus chinensis leaves extract, honeysuckle extract, Dendrobium huoshanense extract and Inula fructus extract respectively;

[0019] The step S2 is replaced by: mixing the extract of Lonicera japonica, the extract of Rhus chinensis leaves, the extract of Lonicera japonica, the extract of Dendrobium huoshanense and the extract of Inula fructus to prepare a mixture, and dissolving the mixture with 4 to 6 times the volume of an ethanol aqueous solution with a volume fraction of 20 to 40%, to prepare a moisturizing and anti-wrinkle composition;

[0020] The Inula japonica extract is prepared by the following operations:

[0021] The Inula flower is crushed, extracted with 80-90% by volume ethanol, filtered, the filtrate is concentrated to obtain an extract, and vacuum freeze-dried to obtain an Inula flower extract.

[0022] Beneficial effects of the present invention:

[0023] The present invention provides a moisturizing and anti-wrinkle composition, which is made of raw materials including honeysuckle, rhus chinensis leaves, honeysuckle, and huoshan dendrobium. When the moisturizing and anti-wrinkle composition is made, the raw materials honeysuckle, rhus chinensis leaves, honeysuckle, and huoshan dendrobium produce a synergistic effect, and the antioxidant performance of the prepared composition is significantly improved: first, the honeysuckle extract and the huoshan dendrobium extract in the composition may interact with the structures of the rhus chinensis leaf extract and the honeysuckle extract, and the structure similar to the conjugated double bond is added to produce a synergistic effect, and the free radical scavenging ability is significantly enhanced, that is, the antioxidant performance is improved, so as to have a good anti-wrinkle effect. Secondly, the honeysuckle extract and the huoshan dendrobium extract have the ability to combine with water in the form of hydrogen bonds, forming a film on the skin surface to prevent skin moisture loss, so they have a good moisturizing effect. Then, honeysuckle extract and Rhus chinensis leaf extract contain hydrophilic phenolic hydroxyl groups, which can combine with water on the skin surface and reduce the evaporation of water in the air; Lonicera japonica extract and Huoshan dendrobium extract contain polar groups such as hydroxyl groups, which can combine with water molecules; and Lonicera japonica extract and Huoshan dendrobium extract are viscous, which can slow down the evaporation of water after forming a film on the skin surface; at the same time, honeysuckle extract and Rhus chinensis leaf extract combine with Fuchsia oleracea extract and Huoshan dendrobium extract at multiple sites on the cell wall surface, and the four work synergistically, combining more tightly with water molecules, enhancing moisturizing properties, and increasing the water content of the skin, thereby protecting the skin and delaying aging.

[0024] Furthermore, the raw materials of the present invention are also added with Inula japonica to prepare a moisturizing and anti-wrinkle composition, which produces a synergistic effect with the honeysuckle fungus, and significantly enhances the free radical scavenging performance of the moisturizing and anti-wrinkle composition. DETAILED DESCRIPTION

[0025] The present invention is further described in detail below in conjunction with specific implementation methods. Example 1

[0026] A moisturizing and anti-wrinkle composition is prepared by the following preparation method:

[0027] 1. Preparation of Lonicera japonica extract: 5 kg of Lonicera japonica raw material was crushed and passed through a 100-mesh sieve, and extracted three times with a 90% ethanol aqueous solution by volume, and the extraction parameters were as follows: extraction temperature 70°C, extraction time 1h, solid-liquid ratio 1:25g / mL; after the extraction was completed, the solid was taken to obtain defatted drug residue, and the defatted drug residue was precipitated with alcohol for 24h, centrifuged, and freeze-dried to obtain the Lonicera japonica extract.

[0028] 2. Preparation of Rhus chinensis leaf extract: 5 kg of Rhus chinensis leaves as raw material were crushed and extracted with 60% by volume ethanol aqueous solution. The extraction parameters were as follows: extraction temperature 55° C., extraction time 0.5 h, and the solid-liquid ratio of the Rhus chinensis leaves to the ethanol aqueous solution was 1 g:12 mL; then the solvent was removed and dried to obtain a crude extract, which was separated using a macroporous resin, eluted with ethanol aqueous solution, concentrated and dried to obtain the Rhus chinensis leaf extract.

[0029] The honeysuckle extract was prepared by the above method, with 5 kg of raw material.

[0030] 3. Preparation of Huoshan Dendrobium officinale extract: The Huoshan Dendrobium officinale extract is prepared by the following operations:

[0031] 5 kg of raw material, Dendrobium huoshanense, was crushed and passed through a 100-mesh sieve, and then water-extracted at a temperature of 70° C. for 1 h, and concentrated under reduced pressure to obtain a crude extract of Dendrobium huoshanense, which was extracted with a 90% by volume ethanol aqueous solution, and the extraction parameters were as follows: extraction temperature of 2° C., extraction time of 24 h, and solid-liquid ratio of 1:10 g / mL; after the extraction, the precipitate was dried, the protein was removed by the sevag method, and then extracted with a 90% by volume ethanol aqueous solution, the method was the same as above, the precipitate was dried, and the Dendrobium huoshanense extract was obtained.

[0032] 4. Mix the honeysuckle extract, Rhus chinensis leaf extract, honeysuckle extract, and Huoshan dendrobium extract to prepare a mixture, and dissolve the mixture in 20% by volume ethanol aqueous solution to prepare a moisturizing and anti-wrinkle composition, wherein the volume ratio of the mixture to the ethanol aqueous solution is 1:4. Example 2

[0033] A moisturizing and anti-wrinkle composition is prepared by the following preparation method:

[0034] 1. Preparation of Lonicera japonica extract: 5 kg of Lonicera japonica raw material was crushed and passed through a 100-mesh sieve, and extracted three times with a 91% ethanol aqueous solution by volume, and the extraction parameters were as follows: extraction temperature 72° C., extraction time 1.5 h, solid-liquid ratio 1:26 g / mL; after the extraction was completed, the solid was taken to obtain the defatted drug residue, and the defatted drug residue was precipitated with alcohol for 24 h, centrifuged, and freeze-dried to obtain the Lonicera japonica extract.

[0035] 2. Preparation of Rhus chinensis leaf extract: 5 kg of Rhus chinensis leaves as raw material were crushed and extracted with 62% ethanol aqueous solution by volume, and the extraction parameters were as follows: extraction temperature 57° C., extraction time 1 h, and the solid-liquid ratio of the Rhus chinensis leaves to the ethanol aqueous solution was 1 g: 14 mL; then the solvent was removed and dried to obtain a crude extract, which was separated with a macroporous resin, eluted with ethanol aqueous solution, concentrated and dried to obtain the Rhus chinensis leaf extract.

[0036] The honeysuckle extract was prepared by the above method, with 5 kg of raw material.

[0037] 3. Preparation of Huoshan Dendrobium officinale extract: The Huoshan Dendrobium officinale extract is prepared by the following operations:

[0038] 5 kg of raw material, Dendrobium huoshanense, was crushed and passed through a 100-mesh sieve, and then extracted with water at a temperature of 72° C. for 1.5 h. The crude extract of Dendrobium huoshanense was obtained by concentration under reduced pressure. The crude extract of Dendrobium huoshanense was extracted with a 92% by volume aqueous ethanol solution. The extraction parameters were as follows: extraction temperature of 2° C., extraction time of 24 h, and solid-liquid ratio of 1:10 g / mL. After the extraction, the precipitate was dried, the protein was removed by the sevag method, and then extracted with a 92% by volume aqueous ethanol solution. The method was the same as above. The precipitate was dried to obtain the Dendrobium huoshanense extract.

[0039] 4. Mix the honeysuckle extract, Rhus chinensis leaf extract, honeysuckle extract, and Dendrobium huoshanense extract to prepare a mixture, and dissolve the mixture in 25% by volume ethanol aqueous solution to prepare a moisturizing anti-wrinkle composition, wherein the volume ratio of the mixture to the ethanol aqueous solution is 1:4.5. Example 3

[0040] A moisturizing and anti-wrinkle composition is prepared by the following preparation method:

[0041] 1. Preparation of Lonicera japonica extract: 5 kg of Lonicera japonica raw material was crushed and passed through a 100-mesh sieve, and extracted with a 92% by volume ethanol aqueous solution for 4 times. The extraction parameters were as follows: extraction temperature 75°C, extraction time 2h, solid-liquid ratio 1:28g / mL; after the extraction, the solid was taken to obtain the defatted residue, and the defatted residue was precipitated with alcohol for 24h, centrifuged, and freeze-dried to obtain the Lonicera japonica extract.

[0042] 2. Preparation of Rhus chinensis leaf extract: 5 kg of Rhus chinensis leaves as raw material were crushed and extracted with 63% ethanol aqueous solution by volume, and the extraction parameters were as follows: extraction temperature 60° C., extraction time 1.7 h, and the solid-liquid ratio of the Rhus chinensis leaves to the ethanol aqueous solution was 1 g:15 mL; then the solvent was removed and dried to obtain a crude extract, which was separated with a macroporous resin, eluted with ethanol aqueous solution, concentrated and dried to obtain the Rhus chinensis leaf extract.

[0043] The honeysuckle extract was prepared by the above method, with 5 kg of raw material.

[0044] 3. Preparation of Huoshan Dendrobium officinale extract: The Huoshan Dendrobium officinale extract is prepared by the following operations:

[0045] 5 kg of raw material, Dendrobium huoshanense, was crushed and passed through a 100-mesh sieve, and then water-extracted at a temperature of 75° C. for 2 h, and concentrated under reduced pressure to obtain a crude extract of Dendrobium huoshanense, which was extracted with a 95% by volume ethanol aqueous solution, and the extraction parameters were as follows: extraction temperature of 2° C., extraction time of 24 h, and solid-liquid ratio of 1:10 g / mL; after the extraction, the precipitate was dried, the protein was removed by the sevag method, and then extracted with a 95% by volume ethanol aqueous solution, the method was the same as above, the precipitate was dried, and the Dendrobium huoshanense extract was obtained.

[0046] 4. Mix the honeysuckle extract, Rhus chinensis leaf extract, honeysuckle extract, and Dendrobium huoshanense extract to prepare a mixture, and dissolve the mixture in 30% by volume ethanol aqueous solution to prepare a moisturizing and anti-wrinkle composition, wherein the volume ratio of the mixture to the ethanol aqueous solution is 1:5. Example 4

[0047] A moisturizing and anti-wrinkle composition is prepared by the following preparation method:

[0048] 1. Preparation of Lonicera japonica extract: 5 kg of Lonicera japonica raw material was crushed and passed through a 100-mesh sieve, and extracted with a 93% ethanol aqueous solution for 5 times. The extraction parameters were as follows: extraction temperature 78° C., extraction time 2.5 h, solid-liquid ratio 1:29 g / mL; after the extraction, the solid was taken to obtain the defatted residue, and the defatted residue was precipitated with alcohol for 24 h, centrifuged, and freeze-dried to obtain the Lonicera japonica extract.

[0049] 2. Preparation of Rhus chinensis leaf extract: 5 kg of Rhus chinensis leaves as raw material were crushed and extracted with 64% ethanol aqueous solution by volume. The extraction parameters were as follows: extraction temperature 62° C., extraction time 2.2 h, and the solid-liquid ratio of the Rhus chinensis leaves to the ethanol aqueous solution was 1 g:16 mL. Then, the solvent was removed and dried to obtain a crude extract. The crude extract was separated with a macroporous resin, eluted with ethanol aqueous solution, concentrated and dried to obtain the Rhus chinensis leaf extract.

[0050] The honeysuckle extract was prepared by the above method, with 5 kg of raw material.

[0051] 3. Preparation of Huoshan Dendrobium officinale extract: The Huoshan Dendrobium officinale extract is prepared by the following operations:

[0052] 5 kg of raw material, Dendrobium huoshanense, was crushed and passed through a 100-mesh sieve, and then water-extracted at a temperature of 78° C. for 1 to 3 h. The crude extract of Dendrobium huoshanense was obtained by concentration under reduced pressure. The crude extract of Dendrobium huoshanense was extracted with a 96% by volume aqueous ethanol solution. The extraction parameters were as follows: extraction temperature of 2° C., extraction time of 24 h, and solid-liquid ratio of 1:10 g / mL. After the extraction, the precipitate was dried, the protein was removed by the sevag method, and then the extract was extracted with a 96% by volume aqueous ethanol solution. The method was the same as above. The precipitate was dried to obtain the Dendrobium huoshanense extract.

[0053] 4. Mix the honeysuckle extract, Rhus chinensis leaf extract, honeysuckle extract, and Huoshan dendrobium extract to prepare a mixture, and dissolve the mixture in 35% by volume ethanol aqueous solution to prepare a moisturizing anti-wrinkle composition, wherein the volume ratio of the mixture to the ethanol aqueous solution is 1:5.5. Example 5

[0054] A moisturizing and anti-wrinkle composition is prepared by the following preparation method:

[0055] 1. Preparation of Lonicera japonica extract: 5 kg of Lonicera japonica raw material was crushed and passed through a 100-mesh sieve, and extracted with a 95% by volume ethanol aqueous solution for 5 times. The extraction parameters were as follows: extraction temperature 80°C, extraction time 3h, solid-liquid ratio 1:30g / mL; after the extraction, the solid was taken to obtain defatted drug residue, and the defatted drug residue was precipitated with alcohol for 24h, centrifuged, and freeze-dried to obtain the Lonicera japonica extract.

[0056] 2. Preparation of Rhus chinensis leaf extract: 5 kg of Rhus chinensis leaves as raw material were crushed and extracted with 65% ethanol aqueous solution by volume, and the extraction parameters were as follows: extraction temperature 65° C., extraction time 3 h, and the solid-liquid ratio of the Rhus chinensis leaves to the ethanol aqueous solution was 1 g:18 mL; then the solvent was removed and dried to obtain a crude extract, which was separated with a macroporous resin, eluted with ethanol aqueous solution, concentrated and dried to obtain the Rhus chinensis leaf extract.

[0057] The honeysuckle extract was prepared by the above method, with 5 kg of raw material.

[0058] 3. Preparation of Huoshan Dendrobium officinale extract: The Huoshan Dendrobium officinale extract is prepared by the following operations:

[0059] 5 kg of raw material, Dendrobium huoshanense, was crushed and passed through a 100-mesh sieve, and then water-extracted at a temperature of 80° C. for 3 h. The crude extract of Dendrobium huoshanense was obtained by concentration under reduced pressure. The crude extract of Dendrobium huoshanense was extracted with a 98% by volume aqueous ethanol solution. The extraction parameters were as follows: extraction temperature of 2° C., extraction time of 24 h, and solid-liquid ratio of 1:10 g / mL. After the extraction, the precipitate was dried, the protein was removed by the sevag method, and then the extract was extracted with a 98% by volume aqueous ethanol solution. The method was the same as above. The precipitate was dried to obtain the Dendrobium huoshanense extract.

[0060] 4. Mix the honeysuckle extract, Rhus chinensis leaf extract, honeysuckle extract, and Dendrobium huoshanense extract to prepare a mixture, and dissolve the mixture in 40% by volume ethanol aqueous solution to prepare a moisturizing and anti-wrinkle composition, wherein the volume ratio of the mixture to the ethanol aqueous solution is 1:6.

[0061] The dosages of the Lonicera japonica extract, Rhus chinensis leaf extract, Lonicera japonica extract, and Dendrobium huoshanense extract in Examples 1 to 5 are shown in Table 1 below:

[0062] Table 1

[0063] Lonicera japonica extract / g Rhus chinensis leaf extract / g Honeysuckle extract / g Huoshan Dendrobium Extract / g Example 1 10 15 15 20 Example 2 12 18 18 28 Example 3 15 22 22 35 Example 4 18 26 26 42 Example 5 20 30 30 50 Example 6

[0064] A moisturizing and anti-wrinkle composition was prepared based on Example 3, except that:

[0065] The raw materials also include Inula flower, which is made into Inula flower extract, and then 15 g of Inula flower extract is added to the mixture. The rest is the same as in Example 3.

[0066] The Inula japonica extract is prepared by the following operations:

[0067] 5 kg of Inula flower was crushed and extracted with 80% ethanol by volume. The extraction parameters were as follows: solid-liquid ratio 1:20 g / mL, extraction time 60 min, extraction once, and filtration after extraction. The filtrate was concentrated to obtain an extract, which was vacuum freeze-dried to obtain an Inula flower extract. Example 7

[0068] A moisturizing and anti-wrinkle composition was prepared based on Example 3, except that:

[0069] The raw materials also include Inula flower, which is made into Inula flower extract, and then 22 g of Inula flower extract is added to the mixture. The rest is the same as in Example 3.

[0070] The Inula japonica extract is prepared by the following operations:

[0071] 5 kg of Inula flower was crushed and extracted with 85% ethanol by volume. The extraction parameters were as follows: solid-liquid ratio 1:20 g / mL, extraction time 60 min, extraction 2 times. After the extraction was completed, the filtrate was filtered and concentrated to obtain an extract, which was vacuum freeze-dried to obtain an Inula flower extract. Example 8

[0072] A moisturizing and anti-wrinkle composition was prepared based on Example 3, except that:

[0073] The raw materials also include Inula flower, which is made into Inula flower extract, and then 30 g of Inula flower extract is added to the mixture. The rest is the same as in Example 3.

[0074] The Inula japonica extract is prepared by the following operations:

[0075] 5 kg of Inula flower was crushed and extracted with 90% ethanol by volume. The extraction parameters were as follows: solid-liquid ratio 1:20 g / mL, extraction time 60 min, extraction 3 times, and filtration after extraction. The filtrate was concentrated to obtain an extract, which was vacuum freeze-dried to obtain an Inula flower extract.

[0076] Comparative Example 1

[0077] A moisturizing and anti-wrinkle composition was prepared based on Example 3, except that:

[0078] The raw materials do not contain Lonicera japonica. In the process of preparing the moisturizing and anti-wrinkle composition, the mixture is replaced with an equal mass of Rhus chinensis leaf extract instead of Lonicera japonica extract, and the rest is the same as in Example 3.

[0079] Comparative Example 2

[0080] A moisturizing and anti-wrinkle composition was prepared based on Example 3, except that:

[0081] The raw material does not contain Rhus chinensis leaves. In the process of preparing the moisturizing and anti-wrinkle composition, the mixture is replaced with an equal mass of honeysuckle extract instead of Rhus chinensis leaf extract, and the rest is the same as in Example 3.

[0082] Comparative Example 3

[0083] A moisturizing and anti-wrinkle composition was prepared based on Example 3, except that:

[0084] The raw material does not contain honeysuckle. In the process of preparing the moisturizing and anti-wrinkle composition, the mixture is replaced with an equal mass of Huoshan Dendrobium extract instead of honeysuckle extract, and the rest is the same as in Example 3.

[0085] Comparative Example 4

[0086] A moisturizing and anti-wrinkle composition was prepared based on Example 3, except that:

[0087] The raw material does not contain Huoshan Dendrobium. In the process of preparing the moisturizing and anti-wrinkle composition, the mixture is replaced with an equal mass of Lonicera japonica extract instead of Huoshan Dendrobium extract, and the rest is the same as in Example 3.

[0088] Comparative Example 5

[0089] A moisturizing and anti-wrinkle composition was prepared based on Example 8, except that:

[0090] The raw materials do not contain Lonicera japonica. In the process of preparing the moisturizing and anti-wrinkle composition, the mixture is replaced with an equal mass of Rhus chinensis leaf extract instead of Lonicera japonica extract, and the rest is the same as in Example 3.

[0091] The moisturizing and anti-wrinkle compositions prepared in Examples 1 to 8 and Comparative Examples 1 to 5 were tested as follows:

[0092] Test Example 1 DPPH free radical scavenging assay

[0093] Weigh 6 mg of DPPH dry powder and dilute to 100 mL with 95% ethanol aqueous solution to prepare 1×10 – 4 mol / L DPPH working solution. Sample and positive control water-soluble V E A sample solution with a mass concentration of 0.2 g / L was prepared with a 95% ethanol aqueous solution. 50 μL of sample solution of different concentrations and 100 μL of DPPH working solution were added to a 96-well plate, and the reaction was protected from light for 30 minutes. The absorbance of the experimental group was read at 517 nm using an enzyme marker. The control group used a 95% ethanol aqueous solution instead of the DPPH working solution. The blank group used a 95% ethanol aqueous solution instead of the sample solution. The DPPH free radical scavenging rate was calculated according to the following formula, and the results are shown in Table 2:

[0094]

[0095] Among them: A 11 is the absorbance of the experimental group; A 21 is the absorbance of the control group; A 31 is the absorbance of the blank group.

[0096] Table 2

[0097] Experimental Group DPPH•Free radical scavenging rate / % Example 1 76.5 Example 2 79.4 Example 3 84.1 Example 4 83.9 Example 5 83.3 Example 6 82.5 Example 7 80.2 Example 8 83.7 Comparative Example 1 52.1 Comparative Example 2 62.5 Comparative Example 3 59.6 Comparative Example 4 55.8 Comparative Example 5 78.6

[0098] As can be seen from Table 2, Examples 1 to 8 provided by the present invention have good DPPH• radical scavenging rates, all reaching more than 79%. The DPPH• radical scavenging rates obtained in Comparative Examples 1 to 4 are decreased to varying degrees compared with Example 3, indicating that a synergistic effect is produced between the raw materials, Lonicera japonica, Rhus chinensis leaves, Lonicera japonica, and Dendrobium huoshanense.

[0099] Test Example 2 Moisturizing performance test

[0100] Prepare the sample and the positive control substance 99% glycerol into 2g / L aqueous solution, take 700μL and apply it on the 3M tape attached to the glass slide, put it into the desiccator together with the saturated aqueous solution of potassium carbonate, keep the humidity at 43%; or put it into the desiccator together with the saturated aqueous solution of ammonium sulfate, keep the humidity at 81%, and weigh it after 2h. The blank control is distilled water, and the moisture retention rate is calculated according to the following formula. The results are shown in Table 3:

[0101]

[0102] Where: M 0 is the mass of the glass sheet, g; M 1 is the mass of the glass slide just after adding the sample, g; M 2 is the mass of the glass piece after being placed for 2 hours, g.

[0103] Table 3

[0104] Experimental Group Moisturizing rate / % Example 1 82.6 Example 2 83.1 Example 3 84.8 Example 4 82.3 Example 5 83.0 Example 6 90.7 Example 7 91.9 Example 8 93.4 Comparative Example 1 69.1 Comparative Example 2 72.5 Comparative Example 3 70.6 Comparative Example 4 55.8 Comparative Example 5 72.1

[0105] As shown in Table 3, Examples 1 to 5 provided by the present invention have good moisture retention rates, all reaching more than 80%. After adding the extract of Inula japonica obtained from Inula japonica as raw material in Examples 6 to 8, the moisture retention rate is significantly improved, reaching more than 90%. The DPPH free radical scavenging rates obtained in Comparative Examples 1 to 4 are reduced to varying degrees compared with Example 3, indicating that the raw materials, Lonicera japonica, Rhus chinensis leaves, Flos lonicerae, and Dendrobium huoshanense, have a synergistic effect. Comparative Example 5 has a significant decrease relative to Example 8, which shows that Inula japonica and Lonicera japonica have a synergistic effect in moisture retention performance.

[0106] The above is only an implementation method of the invention, and does not limit the scope of patent protection. Those skilled in the art can make non-substantial changes or substitutions based on the invention, which still fall within the scope of patent protection.

Claims

1. A moisturizing and anti-wrinkle composition, characterized in that: Made from the following raw materials in parts by weight: 10-20 parts of honeysuckle phyllostachys, 15-30 parts of Rhus chinensis leaves, 15-30 parts of honeysuckle flowers and 20-50 parts of Huoshan dendrobium; The preparation method of the composition comprises the following steps: S1: taking raw materials of Lonicera japonica, Rhus chinensis leaves, honeysuckle and Dendrobium huoshanense to prepare Lonicera japonica extract, Rhus chinensis leaves extract, honeysuckle extract and Dendrobium huoshanense extract respectively; S2: mixing the extract of Lonicera japonica, the extract of Rhus chinensis leaves, the extract of Honeysuckle and the extract of Dendrobium huoshanense to prepare a mixture, and dissolving the mixture in an ethanol aqueous solution with a volume fraction of 20-60% to prepare a moisturizing and anti-wrinkle composition; in, The extract of the honeysuckle phylloxera is prepared by the following operations: crushing the raw material of the honeysuckle phylloxera, extracting it with an ethanol aqueous solution with a volume fraction of 90-95%, taking the solid to obtain defatted medicinal residue after the extraction is completed, and precipitating the defatted medicinal residue with alcohol, centrifuging, and freeze-drying to obtain the honeysuckle phylloxera extract; The Rhus chinensis leaf extract is prepared by the following operations: crushing the raw material Rhus chinensis leaves, extracting with an ethanol aqueous solution with a volume fraction of 60-65%, then removing the solvent, drying to obtain a crude extract, separating the crude extract with a macroporous resin, eluting with an ethanol aqueous solution, concentrating and drying to obtain the Rhus chinensis leaf extract; The honeysuckle extract is prepared by the following operations: crushing the raw honeysuckle, leaching with an ethanol aqueous solution with a volume fraction of 60-65%, then removing the solvent, drying to obtain a crude extract, separating the crude extract with a macroporous resin, eluting with an ethanol aqueous solution, concentrating and drying to obtain the honeysuckle extract; The Huoshan Dendrobium extract is prepared by the following operations: crushing the raw material Huoshan Dendrobium, then extracting with water, and concentrating under reduced pressure to obtain a Huoshan Dendrobium crude extract, extracting the Huoshan Dendrobium crude extract with an ethanol aqueous solution with a volume fraction of 90-98%, drying the precipitate, removing protein, and then extracting with an ethanol aqueous solution with a volume fraction of 90-98%, and drying the precipitate to obtain the Huoshan Dendrobium extract.

2. The composition according to claim 1, characterized in that The material-liquid ratio of the raw material Rhus chinensis leaves to the ethanol aqueous solution is 1 g: 12-18 mL.

3. The composition according to claim 1, characterized in that The material-liquid ratio of the raw material honeysuckle and the ethanol aqueous solution is 1g:12-18mL.

4. A moisturizing and anti-wrinkle composition, characterized in that: Made from the following raw materials in parts by weight: 10-20 parts of honeysuckle phyllostachys, 15-30 parts of Rhus chinensis leaves, 15-30 parts of honeysuckle, 20-50 parts of Huoshan dendrobium and 15-30 parts by weight of Inula flower; The preparation method of the composition comprises the following steps: S1: taking raw materials of Lonicera japonica, Rhus chinensis leaves, honeysuckle, Dendrobium huoshanense and Inula flos roxburghii to prepare Lonicera japonica extract, Rhus chinensis leaves extract, honeysuckle extract, Dendrobium huoshanense extract and Inula flos roxburghii extract respectively; S2: mixing the extract of Lonicera japonica, the extract of Rhus chinensis leaves, the extract of Lonicera japonica, the extract of Dendrobium huoshanense and the extract of Inula japonica to prepare a mixture, and dissolving the mixture in an ethanol aqueous solution with a volume fraction of 20-40% to prepare a moisturizing and anti-wrinkle composition; in, The extract of the honeysuckle phylloxera is prepared by the following operations: crushing the raw material of the honeysuckle phylloxera, extracting it with an ethanol aqueous solution with a volume fraction of 90-95%, taking the solid to obtain defatted medicinal residue after the extraction is completed, and precipitating the defatted medicinal residue with alcohol, centrifuging, and freeze-drying to obtain the honeysuckle phylloxera extract; The Rhus chinensis leaf extract is prepared by the following operations: crushing the raw material Rhus chinensis leaves, extracting with an ethanol aqueous solution with a volume fraction of 60-65%, then removing the solvent, drying to obtain a crude extract, separating the crude extract with a macroporous resin, eluting with an ethanol aqueous solution, concentrating and drying to obtain the Rhus chinensis leaf extract; The honeysuckle extract is prepared by the following operations: crushing the raw honeysuckle, leaching with an ethanol aqueous solution with a volume fraction of 60-65%, then removing the solvent, drying to obtain a crude extract, separating the crude extract with a macroporous resin, eluting with an ethanol aqueous solution, concentrating and drying to obtain the honeysuckle extract; The Huoshan Dendrobium extract is prepared by the following operations: crushing the raw material Huoshan Dendrobium, then extracting with water, and concentrating under reduced pressure to obtain a Huoshan Dendrobium crude extract, extracting the Huoshan Dendrobium crude extract with an ethanol aqueous solution with a volume fraction of 90-98%, drying the precipitate, removing protein, and then extracting with an ethanol aqueous solution with a volume fraction of 90-98%, and drying the precipitate to obtain the Huoshan Dendrobium extract; The Inula japonica extract is prepared by the following operations: crushing the raw material Inula japonica, extracting with 80-90% ethanol by volume, filtering, concentrating the filtrate to obtain an extract, and vacuum freeze-drying to obtain the Inula japonica extract.

5. The composition according to claim 4, characterized in that The volume ratio of the mixture to the ethanol aqueous solution in S2 is 1:4-6.

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