A composition containing probiotics and a probiotic pressed candy prepared using the same

Abstract

The application belongs to the technical field of food, and discloses a composition containing probiotics and probiotic compressed sugar prepared by using the composition. The composition contains sorbitol, isomalt, vitamin C, prebiotics and probiotic powder. The prebiotics contain at least one of xylitol, inulin and stachyose. The composition containing probiotics provided by the application contains sorbitol, isomalt, vitamin C, prebiotics and probiotic powder as main components. By adding isomalt and sorbitol, two low-hygroscopic sugar alcohols, and by selecting prebiotics, the survival rate of probiotics can be effectively improved. The probiotic compressed sugar prepared by using the composition is easy to store and has a long shelf life. The probiotic compressed sugar provided by the application has a smooth, clean and intact surface, uniform color and no impurities.

Description

Technical Field

[0001] This invention belongs to the field of food technology, specifically relating to a composition containing probiotics and probiotic compressed sugar prepared therefrom. Background Technology

[0002] Probiotics are live microorganisms that are beneficial to the host and, when ingested in sufficient quantities, can have a positive impact on the host's health. They are normally found in the human gut, helping to maintain the balance of the gut microbiota and promote digestive health. There are many types of probiotics, the most common being Lactobacillus, Bifidobacterium, and yeast. The functions of probiotics include: aiding digestion: probiotics can break down indigestible fiber and other components in food, promoting nutrient absorption; enhancing the immune system: probiotics help regulate the immune response and enhance the body's resistance to disease; maintaining gut health: probiotics can inhibit the growth of harmful bacteria, reduce intestinal inflammation, and prevent intestinal diseases; and improving mental health: some studies suggest that probiotics may have a positive impact on mood and cognitive function, possibly related to the "gut-brain axis."

[0003] Probiotic compressed tablets are food supplements containing probiotics, typically available in candy form for easy portability and consumption. They combine the health benefits of probiotics with the portability of candy, aiming to provide a delicious and healthy way to consume probiotics. Their features include high portability, good taste, and easy dosage control. Currently, there are many probiotic compressed tablets on the market, but the survival rate of probiotics during production, storage, and digestion cannot be guaranteed. For example, a probiotic compressed tablet with isomaltitol, mannitol, milk powder, and prebiotics as its main ingredients can maintain a high survival rate for the first month after compression, but this rate drops sharply in the third month, making it impossible to guarantee the shelf life of the probiotic compressed tablet.

[0004] Therefore, providing a probiotic composition that is beneficial to improving the survival rate of probiotics is of great significance to the probiotic tablet industry. Summary of the Invention

[0005] The present invention aims to at least solve one of the technical problems existing in the prior art. To this end, the present invention provides a probiotic-containing composition and probiotic compressed tablets prepared therefrom. The probiotic-containing composition provided by the present invention can effectively improve the survival rate of probiotics, and the probiotic compressed tablets prepared therefrom are easy to store and have a long shelf life.

[0006] This invention provides a composition containing probiotics.

[0007] Specifically, a probiotic-containing composition includes sorbitol, isomaltitol, vitamin C, prebiotics, and probiotic powder; said prebiotics include at least one of xylitol, inulin, and stachyose.

[0008] The addition of sorbitol and isomalt not only increases the sweetness of the composition but also enhances its stability. By adding sorbitol, isomalt, and the prebiotic xylitol—all three are low-hygroscopic sugar alcohols—and when used in conjunction with vitamin C, they help maintain the low water activity of the probiotic compressed candy, thereby reducing bacterial loss during the compression process.

[0009] Preferably, the prebiotic comprises at least two of xylitol, inulin, and stachyose.

[0010] Preferably, the prebiotic comprises xylitol, inulin, and stachyose. More preferably, the mass ratio of xylitol, inulin, and stachyose is (0.5–1.5):(0.5–2):(0.1–2); more preferably, the mass ratio of xylitol, inulin, and stachyose is (0.8–1.2):(0.8–1.2):(0.5–1.5). Using xylitol, inulin, and stachyose as prebiotics can further enhance the viability stability of probiotics during compression and storage, and reduce viability loss.

[0011] Preferably, the composition, by weight, comprises 30-60 parts sorbitol, 15-30 parts isomaltitol, 1-8 parts vitamin C, 5-20 parts prebiotics, and 0.5-15 parts probiotic powder. More preferably, the composition, by weight, comprises 30-50 parts sorbitol, 20-25 parts isomaltitol, 4-8 parts vitamin C, 10-20 parts prebiotics, and 3-15 parts probiotic powder.

[0012] Preferably, the composition further comprises a filler. The filler includes at least one selected from fruit powder and maltodextrin. The fruit powder can improve the appearance and flavor of the composition tablets. The fruit powder includes, but is not limited to, at least one selected from cranberry powder, strawberry powder, blueberry powder, orange powder, dragon fruit powder, peach powder, cherry powder, and apple powder.

[0013] Preferably, the composition contains 8 to 15 parts by weight of filler.

[0014] Preferably, the composition contains 0.5 to 2 parts by weight of magnesium stearate.

[0015] Preferably, the composition contains 0.5 to 1.5 parts by weight of edible flavoring.

[0016] In this invention, the probiotic powder includes *Lactobacillus plantarum* Lp90, *Bifidobacterium animalis* subsp. *lactobacter* BLa36, *Lactobacillus plantarum* Lp05, *Lactobacillus rhamnosus* LRa66, *Lactobacillus plantarum* Lp99, *Lactobacillus acidophilus* LA16, *Lactobacillus casei* LC07, *Lactobacillus paracasei* LC28, *Lactobacillus reuteri* LR47, *Lactobacillus salivarius* LS09, *Lactobacillus gasseri* LG24, *Bifidobacterium bifidum* BBi77, and *Lactobacillus longiflorus*. The probiotic powder contains at least one of the following: *Bifidobacterium longum* BL44, *Bifidobacterium breve* BBr02, *Bifidobacterium adolescentis* BAC30, *Bifidobacterium infantis* B145, *Streptococcus salivarius* subsp. *thermophilus* ST11, *Lactobacillus delbrueckii* subsp. *bulgaricus* LB50, *Lactococcus lactis* subsp. *lactophoresis* LLa61, *Pediococcus lactis* PA53, *Lactobacillus plantarum* CW006, *Lactobacillus gasseri* LG08, *Lactobacillus paracasei* Lpc-37, and *Lactobacillus rhamnosus* Lr-32. When the probiotic powder contains the above-mentioned specific probiotics, the viable count after tableting reaches 50 billion to 100 billion CFU / g. In one embodiment, the probiotic powder includes *Lactobacillus plantarum* Lp90, *Bifidobacterium animalis* subsp. *lactobacter* BLa36, *Lactobacillus plantarum* Lp05, *Lactobacillus rhamnosus* LRa66, *Lactobacillus plantarum* Lp99, *Lactobacillus acidophilus* LA16, *Lactobacillus casei* LC07, *Lactobacillus paracasei* LC28, *Lactobacillus reuteri* LR47, *Lactobacillus salivarius* LS09, *Lactobacillus gasseri* LG24, *Bifidobacterium bifidum* BBi77, *Bifidobacterium longum* subsp. *longum* BL44, and *Bifidobacterium breve* BBr0. 2. At least one of the following: Bifidobacterium adolescentis BAC30, Bifidobacterium longum subsp. infantis B145, Streptococcus salivarius subsp. thermophilus ST11, Lactobacillus delbrueckii subsp. bulgaricus LB50, Lactococcus lactis subsp. lactis LLa61, Pediococcus lactis PA53, Lactobacillus plantarum CW006, Lactobacillus gasseri LG08, Lactobacillus paracasei Lpc-37, and Lactobacillus rhamnosus Lr-32, to achieve a higher viable bacteria retention rate of 50%–92% when the resulting probiotic-containing composition is stored at 25°C for 3 months.

[0017] Preferably, the probiotic powder is freeze-dried probiotic powder. Freeze-dried probiotic powder has a high bacterial activity.

[0018] Preferably, the probiotic powder is added to a concentration of 100 billion CFU / g based on the live bacteria ratio.

[0019] The present invention also provides a method for preparing a composition containing probiotics.

[0020] Specifically, a method for preparing a composition containing probiotics includes the following steps:

[0021] The prebiotic and probiotic powders were mixed and dispersed to obtain the first mixture; the sorbitol, isomaltitol, and vitamin C were mixed and dispersed, and then sieved to obtain the second mixture.

[0022] The first mixture and the second mixture are mixed and dispersed, sieved, and compressed into tablets to obtain a composition containing probiotics.

[0023] When the composition contains a filler, the preparation method includes the following steps:

[0024] The prebiotic and probiotic powders were mixed and dispersed, and then the filler was added and mixed and dispersed to obtain the first mixture; the sorbitol, isomaltitol and vitamin C were mixed and dispersed, and then sieved to obtain the second mixture.

[0025] The first mixture and the second mixture are mixed and dispersed, sieved, and compressed into tablets to obtain a composition containing probiotics.

[0026] When the composition simultaneously contains a filler, a fragrance, and magnesium stearate, the preparation method includes the following steps:

[0027] The prebiotic and probiotic powders were mixed and dispersed, and then the filler was added and mixed and dispersed to obtain the first mixture; the sorbitol, isomaltitol and vitamin C were mixed and dispersed, and then sieved to obtain the second mixture.

[0028] The first and second mixtures are mixed and dispersed, then flavoring and magnesium stearate are added and mixed and dispersed, sieved, and compressed into tablets to obtain a composition containing probiotics.

[0029] Preferably, the moisture content of the sorbitol, isomaltitol, prebiotic, vitamin C, and filler is controlled to be within 3 wt%.

[0030] Preferably, the sorbitol, isomaltitol, and prebiotics are all passed through a 40-mesh sieve, and the filler is passed through a 60-mesh sieve.

[0031] It should be noted that the mixing and dispersion in the preparation method does not have special requirements, as long as the mixing is thorough. The tableting process can use conventional food or pharmaceutical tableting methods, but it is necessary to control the ambient temperature below 18°C ​​and humidity below 35% RH during tableting to reduce the inactivation of probiotics. If excessive heat is generated during the tableting process, cooled compressed air or nitrogen can be used to purge the work surface to lower the temperature.

[0032] The present invention also provides a probiotic compressed tablet.

[0033] Specifically, a probiotic compressed tablet comprises the above-mentioned probiotic-containing composition.

[0034] Preferably, the probiotic compressed tablets weigh 1.0 to 2.0 g per tablet.

[0035] Preferably, the probiotic compressed tablets are packaged in a moisture-proof material to reduce the impact of water activity on bacterial activity during storage and ensure minimal loss of bacterial activity within the shelf life. Preferably, the probiotic compressed tablets are packaged in PVC aluminum foil blister packs, PET bottles, or aluminum foil composite films. Compared to packaging in PET bottles or aluminum foil composite films, when the probiotic compressed tablets are packaged in PVC aluminum foil blister packs, the water activity is lower during storage, which is more beneficial for ensuring the stability of bacterial activity.

[0036] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0037] The probiotic-containing composition provided by this invention uses sorbitol, isomaltitol, vitamin C, prebiotics, and probiotic powder as its main components. By adding isomaltitol and sorbitol, two low-hygroscopic sugar alcohols, and by selecting appropriate prebiotics, the survival rate of probiotics can be effectively improved. The probiotic compressed tablets prepared using this composition are easy to store and have a long shelf life. The addition of isomaltitol and sorbitol helps maintain the low water activity of the probiotic compressed tablets, while xylitol, inulin, and stachyose, as prebiotics, can protect the probiotics and improve their survival rate and stability during compression and storage.

[0038] The probiotic compressed tablets provided by this invention have a smooth, clean, and uniform color, and are free of impurities. Attached Figure Description

[0039] Figure 1 This is an image of the probiotic compressed tablets prepared in Example 9. Detailed Implementation

[0040] To enable those skilled in the art to more clearly understand the technical solutions described in this invention, the following embodiments are provided for illustration. It should be noted that the following embodiments do not constitute a limitation on the scope of protection claimed by this invention.

[0041] The moisture content of sorbitol, isomaltitol, lactose, vitamin C, xylitol, inulin, stachyose, fructooligosaccharides, isomaltooligosaccharides, cranberry powder, blueberry powder, orange powder, maltodextrin, flavoring, and magnesium stearate used in the following examples and comparative examples is controlled to be less than 3 wt%; the probiotic powder is freeze-dried probiotic powder; all raw materials used are commercially available and can be obtained from conventional commercial channels or by existing known methods.

[0042] Example 1

[0043] A probiotic-containing composition comprising the following components in parts by weight: 38 parts sorbitol, 22 parts isomaltitol, 5 parts vitamin C, 15 parts prebiotics, 10 parts cranberry fruit powder (filler), 8 parts probiotic powder, 1 part flavoring, and 1 part magnesium stearate. The prebiotics consist of 5 parts xylitol, 5 parts inulin, and 5 parts stachyose. The probiotic powder comprises *Lactobacillus plantarum* Lp90, *Bifidobacterium animalis* subsp. *lactobacterium* BLa36, *Lactobacillus plantarum* Lp05, *Lactobacillus rhamnosus* LRa66, *Lactobacillus plantarum* Lp99, *Lactobacillus acidophilus* LA16, *Lactobacillus casei* LC07, and *Lactobacillus paracasei* LC. 28. This probiotic compressed candy is composed of *Lactobacillus reuteri* LR47, *Lactobacillus salivarius* LS09, *Lactobacillus gasseri* LG24, *Bifidobacterium bifidum* BBi77, *Bifidobacterium longum* subsp. *longum* BL44, *Bifidobacterium breve* BBr02, *Bifidobacterium adolescentis* BAC30, *Bifidobacterium longum* subsp. *infant* B145, *Streptococcus salivarius* subsp. *thermophilus* ST11, *Lactobacillus delbrueckii* subsp. *bulgaricus* LB50, *Lactococcus lactis* subsp. *lactophoresis* LLa61, *Pediococcus lactis* PA53, *Lactobacillus plantarum* CW006, *Lactobacillus gasseri* LG08, *Lactobacillus paracasei* Lpc-37, and *Lactobacillus rhamnosus* Lr-32. The preparation method of this probiotic compressed candy includes the following steps:

[0044] Mix and disperse the prebiotic and probiotic powder for 5 minutes, then add the filler and mix and disperse for another 5 minutes to obtain the first mixture;

[0045] Sorbitol, isomaltitol and vitamin C were mixed and dispersed for 20 minutes, and then passed through a 40-mesh sieve to obtain a second mixture;

[0046] Mix and disperse the first and second mixtures for 5 minutes, then add the flavoring and magnesium stearate and mix and disperse for 10 minutes. Pass the mixture through a 40-mesh sieve to obtain the third mixture.

[0047] The third mixture was placed in an environment of 4°C and cooled for 24 hours.

[0048] The cooled third mixture is pressed into tablets in a clean room using a 12mm×12mm square mold. The ambient temperature is <18℃ and the humidity is <35%RH. The filling amount is adjusted to stabilize the tablet weight at 1.5g / tablet. The pressing pressure is adjusted to 10kN and the rotation speed is 15rpm. Because heat is generated during the pressing process, the turntable needs to be purged with cooled compressed air every half hour to cool it down. The material is collected into sterile plastic turnover bags and sealed.

[0049] The resulting tablets were packaged in PVC aluminum foil blister packs at an ambient temperature of <18℃ and humidity of <35%RH.

[0050] Example 2

[0051] The difference between Example 2 and Example 1 is that the prebiotic content is 20 parts, specifically including 5 parts xylitol, 5 parts inulin, and 10 parts stachyose. The preparation method is the same as in Example 1.

[0052] Example 3

[0053] The difference between Example 3 and Example 1 is that the prebiotic content is 15 parts, specifically including 7.5 parts xylitol and 7.5 parts inulin. The preparation method is the same as in Example 1.

[0054] Table 1 shows the components and dosages for Examples 4-8 and Comparative Examples 1-6. The preparation methods are the same as in Example 1.

[0055] Table 1. Components and dosages for each embodiment and comparative example.

[0056]

[0057] Product effectiveness test

[0058] The probiotic-containing compositions prepared in each example and comparative example were tested separately. Each composition was stored at 25°C for 1 to 3 months, and the number of live bacteria in each composition at different times was detected and the live bacteria retention rate was calculated. The test results are shown in Table 2.

[0059] Wherein, the viable bacteria retention rate after tableting = number of viable bacteria after tableting / number of viable bacteria added before tableting × 100%;

[0060] 1-month viable bacteria retention rate = (number of viable bacteria after 1 month of storage / number of viable bacteria added before tableting) × 100%;

[0061] 3-month viable bacteria retention rate = number of viable bacteria after 3 months of storage / number of viable bacteria added before tableting × 100%.

[0062] Table 2

[0063]

[0064] As shown in Table 2, the composition prepared in the embodiments of the present invention exhibits a high survival rate of probiotics after 3 months of storage, with a live bacteria retention rate as high as 81.96%. The composition prepared in the comparative example is significantly lower than that in the embodiments.

Claims

1. A composition containing probiotics, characterized in that, By weight, it contains 30-50 parts sorbitol, 20-25 parts isomaltitol, 4-8 parts vitamin C, 10-20 parts prebiotics, and 3-15 parts probiotic powder; the prebiotics include xylitol, inulin, and stachyose; the mass ratio of xylitol, inulin, and stachyose is (0.8-1.2):(0.8-1.2):(0.5-1.5).

2. The composition according to claim 1, characterized in that, The composition further comprises a filler; the filler includes at least one selected from fruit powder and maltodextrin.

3. The composition according to claim 2, characterized in that, The filler is 8 to 15 parts by weight.

4. The composition according to claim 2 or 3, characterized in that, The composition contains 0.5 to 2 parts by weight of magnesium stearate.

5. The method for preparing the probiotic-containing composition according to claim 1, characterized in that, Includes the following steps: The prebiotic and probiotic powders were mixed and dispersed to obtain the first mixture; the sorbitol, isomaltitol, and vitamin C were mixed and dispersed, and then sieved to obtain the second mixture. The first mixture and the second mixture are mixed and dispersed, sieved, and compressed into tablets to obtain a composition containing probiotics.

6. A probiotic compressed tablet, characterized in that, A composition comprising any one of claims 1 to 4 containing probiotics.

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