A hybrid waxy sorghum breeding method

Abstract

The application discloses a hybrid waxy sorghum breeding method, comprising the following steps: step one, selecting sorghum plants with good growth and flowering, and waiting for use; step two, pretreating the sorghum plants: spraying a pretreatment agent on the sorghum plants, then bagging overnight, and taking out the bag. The hybrid waxy sorghum breeding method adopts the pretreatment agent treatment on the sorghum plants, and the zinc sulfate solution, the iron sulfate solution and the polyaspartic acid are cooperated and then treated by the montmorillonite adjustment and improvement, so that the interlayer spacing of the lamella is optimized; then the sodium dodecyl benzene sulfonate solution is uniformly mixed, so that the active efficiency is optimized; the zinc sulfate solution, the iron sulfate solution and other systems are cooperated, so that the activity of the system on the sorghum plants is enhanced, and the treatment of the detasseling treatment liquid is facilitated; the adjusting liquid added in the detasseling treatment liquid is treated by the graphene cooperation, and then the pretreatment agent treatment can be cooperated, so that the detasseling rate and the seed setting rate of the product can be improved.

Description

Technical Field

[0001] This invention relates to the field of hybrid glutinous sorghum technology, specifically to a method for breeding hybrid glutinous sorghum. Background Technology

[0002] Glutinous sorghum has a high amylopectin content in its total starch content, making it a major raw material for brewing baijiu (Chinese liquor). Traditional famous baijiu brands in my country, such as "Luzhou Laojiao," "Maotai," and "Langjiu," all use glutinous sorghum as their main ingredient. Baijiu brewed from glutinous sorghum is mellow, rich, fragrant, and sweet. In contrast, conventional glutinous sorghum varieties, such as Qingke Yanggaoliang, have poor resistance to pests and diseases and have low yields, generally producing only 3,000-4,500 kilograms per hectare. Their total production is far from meeting the demand for brewing famous baijiu using traditional techniques, and the annual supply gap of glutinous sorghum is over 1 million tons.

[0003] The existing hybrid breeding process for glutinous sorghum is simple, and the raw materials used in the emasculation process are relatively common, which affects the emasculation rate and seed setting rate. Based on this, the present invention further improves the process. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the purpose of this invention is to provide a method for breeding hybrid glutinous sorghum to solve the problems mentioned in the background art.

[0005] The present invention solves the technical problem by adopting the following technical solution:

[0006] This invention provides a method for breeding hybrid glutinous sorghum, comprising the following steps:

[0007] Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use;

[0008] Step 2, Pretreatment of sorghum plants:

[0009] Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags.

[0010] Step 3: The pre-treated sorghum plants are bagged with the emasculation treatment solution and left to stand overnight. Japonica sorghum iron 239B is used as the female parent and glutinous sorghum maintainer line 72B is used as the male parent for hybridization. The hybrid offspring are then self-pollinated and selected to develop a stable glutinous sorghum maintainer line B, thus completing the breeding of hybrid glutinous sorghum. The emasculation treatment solution is sprayed into the bag under high pressure to form a mist-like emasculation treatment solution.

[0011] Preferably, the pretreatment agent is prepared by:

[0012] S01: Improvement treatment of montmorillonite:

[0013] S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 4-7% of polyaspartic acid (total amount of zinc sulfate solution) and 2-5% of thermally modified montmorillonite (total amount of zinc sulfate solution), stir and mix thoroughly to obtain the pretreatment agent.

[0014] Preferably, the zinc sulfate solution has a mass fraction of 24-28%; the ferric sulfate solution has a mass fraction of 20-25%.

[0015] Preferably, the specific steps of the montmorillonite conditioning and improvement treatment are as follows:

[0016] Montmorillonite is first heat-treated at 310-330℃ for 10-15 minutes, then cooled to 180-185℃ at a rate of 2-4℃ / min, held for 5-10 minutes, and finally cooled to 60℃ at a rate of 1-3℃ / min.

[0017] Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried.

[0018] Preferably, the sodium dodecylbenzenesulfonate solution has a mass fraction of 10-15%.

[0019] Preferably, the method for preparing the virginity-determination solution is as follows:

[0020] Mix 1-3 parts ethephon, 1-3 parts gibberellin and 1-3 parts benzsulfuron thoroughly, then add to 4-7 parts conditioning solution and mix thoroughly to obtain the maleicide treatment solution.

[0021] Preferably, the preparation method of the conditioning liquid is as follows:

[0022] S101: Add graphene to a 3-6 times solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed.

[0023] S102: Place the product of S01 in 2-4 times its volume of chitosan solution and stir thoroughly to obtain the conditioning solution.

[0024] Preferably, the sodium lignosulfonate solution has a mass fraction of 10-15%.

[0025] Preferably, the ultrasonic dispersion power is 350-450W and the dispersion time is 30-40min.

[0026] Preferably, the chitosan solution has a mass fraction of 2-5%.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The breeding of hybrid glutinous sorghum in this invention involves pretreatment of sorghum plants with a zinc sulfate solution, ferric sulfate solution, and polyaspartic acid, followed by conditioning with montmorillonite. The montmorillonite is then heat-treated at 10-330℃ for 10-15 minutes, cooled to 180-185℃ at a rate of 2-4℃ / min, held for 5-10 minutes, and finally cooled to 60℃ at a rate of 1-3℃ / min to optimize the interlamellar spacing. The mixture is then mixed with sodium dodecylbenzenesulfonate solution to further optimize its activity. By incorporating this into the zinc sulfate and ferric sulfate solution system, the system's activity on the sorghum plants is enhanced, facilitating the emasculation treatment. The conditioning solution added to the emasculation treatment is co-treated with graphene, which synergistically enhances the effects of the pretreatment, resulting in a coordinated improvement in the emasculation rate and seed setting rate of the product. Detailed Implementation

[0029] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to specific examples. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0030] The method for breeding hybrid glutinous sorghum according to this embodiment includes the following steps:

[0031] Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use;

[0032] Step 2, Pretreatment of sorghum plants:

[0033] Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags.

[0034] Step 3: The pre-treated sorghum plants are bagged with the emasculation treatment solution and left to stand overnight. Japonica sorghum iron 239B is used as the female parent and glutinous sorghum maintainer line 72B is used as the male parent for hybridization. The hybrid offspring are then self-pollinated and selected to develop a stable glutinous sorghum maintainer line B, thus completing the breeding of hybrid glutinous sorghum. The emasculation treatment solution is sprayed into the bag under high pressure to form a mist-like emasculation treatment solution.

[0035] The preparation method of the pretreatment agent in this embodiment is as follows:

[0036] S01: Improvement treatment of montmorillonite:

[0037] S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 4-7% of polyaspartic acid (total amount of zinc sulfate solution) and 2-5% of thermally modified montmorillonite (total amount of zinc sulfate solution), stir and mix thoroughly to obtain the pretreatment agent.

[0038] In this embodiment, the zinc sulfate solution has a mass fraction of 24-28%, and the ferric sulfate solution has a mass fraction of 20-25%.

[0039] The specific operational steps for the montmorillonite conditioning and improvement treatment in this embodiment are as follows:

[0040] Montmorillonite is first heat-treated at 310-330℃ for 10-15 minutes, then cooled to 180-185℃ at a rate of 2-4℃ / min, held for 5-10 minutes, and finally cooled to 60℃ at a rate of 1-3℃ / min.

[0041] Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried.

[0042] The sodium dodecylbenzenesulfonate solution in this embodiment has a mass fraction of 10-15%.

[0043] The preparation method of the emasculation treatment solution in this embodiment is as follows:

[0044] Mix 1-3 parts ethephon, 1-3 parts gibberellin and 1-3 parts benzsulfuron thoroughly, then add to 4-7 parts conditioning solution and mix thoroughly to obtain the maleicide treatment solution.

[0045] The preparation method of the conditioning liquid in this embodiment is as follows:

[0046] S101: Add graphene to a 3-6 times solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed.

[0047] S102: Place the product of S01 in 2-4 times its volume of chitosan solution and stir thoroughly to obtain the conditioning solution.

[0048] The sodium lignosulfonate solution in this embodiment has a mass fraction of 10-15%.

[0049] In this embodiment, the ultrasonic dispersion power is 350-450W and the dispersion time is 30-40min.

[0050] The chitosan solution in this embodiment has a mass fraction of 2-5%.

[0051] Example 1.

[0052] The method for breeding hybrid glutinous sorghum according to this embodiment includes the following steps:

[0053] Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use;

[0054] Step 2, Pretreatment of sorghum plants:

[0055] Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags.

[0056] Step 3: Use bags containing the emasculation treatment solution to cover the pretreated sorghum plants and continue overnight treatment. Use japonica sorghum iron 239B as the female parent and glutinous sorghum maintainer line 72B as the male parent for hybridization. After self-pollination and selection of the hybrid offspring, a stable glutinous sorghum maintainer line B is bred, thus completing the breeding of hybrid glutinous sorghum.

[0057] The preparation method of the pretreatment agent in this embodiment is as follows:

[0058] S01: Improvement treatment of montmorillonite:

[0059] S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 4% of polyaspartic acid (total of zinc sulfate solution) and 2% of thermally modified montmorillonite (total of zinc sulfate solution), stir and mix thoroughly to obtain the pretreatment agent.

[0060] In this embodiment, the zinc sulfate solution has a mass fraction of 24% and the ferric sulfate solution has a mass fraction of 20%.

[0061] The specific operational steps for the montmorillonite conditioning and improvement treatment in this embodiment are as follows:

[0062] Montmorillonite was first heat-treated at 310℃ for 10 minutes, then cooled to 180℃ at a rate of 2℃ / min, held for 5 minutes, and finally cooled to 60℃ at a rate of 1℃ / min.

[0063] Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried.

[0064] The sodium dodecylbenzenesulfonate solution in this embodiment has a mass fraction of 10%.

[0065] The preparation method of the emasculation treatment solution in this embodiment is as follows:

[0066] Mix 1 part ethephon, 1 part gibberellin and 1 part benzsulfuron thoroughly, then add to 4 parts conditioning solution and mix thoroughly to obtain the maserization treatment solution.

[0067] The preparation method of the conditioning liquid in this embodiment is as follows:

[0068] S101: Add graphene to a 3-fold solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed.

[0069] S102: Place the product of S01 into 2 times the amount of chitosan solution and stir thoroughly to obtain the conditioning solution.

[0070] The sodium lignosulfonate solution in this embodiment has a mass fraction of 10%.

[0071] In this embodiment, the ultrasonic dispersion power is 350W and the dispersion time is 30min.

[0072] The chitosan solution in this embodiment has a mass fraction of 2%.

[0073] Example 2.

[0074] The method for breeding hybrid glutinous sorghum according to this embodiment includes the following steps:

[0075] Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use;

[0076] Step 2, Pretreatment of sorghum plants:

[0077] Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags.

[0078] Step 3: Use bags containing the emasculation treatment solution to cover the pretreated sorghum plants and continue overnight treatment. Use japonica sorghum iron 239B as the female parent and glutinous sorghum maintainer line 72B as the male parent for hybridization. After self-pollination and selection of the hybrid offspring, a stable glutinous sorghum maintainer line B is bred, thus completing the breeding of hybrid glutinous sorghum.

[0079] The preparation method of the pretreatment agent in this embodiment is as follows:

[0080] S01: Improvement treatment of montmorillonite:

[0081] S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 7% of polyaspartic acid (total of zinc sulfate solution) and 5% of thermally modified montmorillonite (total of zinc sulfate solution), stir and mix thoroughly to obtain the pretreatment agent.

[0082] In this embodiment, the zinc sulfate solution has a mass fraction of 28% and the ferric sulfate solution has a mass fraction of 25%.

[0083] The specific operational steps for the montmorillonite conditioning and improvement treatment in this embodiment are as follows:

[0084] Montmorillonite was first heat-treated at 330℃ for 15 minutes, then cooled to 185℃ at a rate of 4℃ / min, held for 10 minutes, and finally cooled to 60℃ at a rate of 3℃ / min.

[0085] Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried.

[0086] The sodium dodecylbenzenesulfonate solution in this embodiment has a mass fraction of 15%.

[0087] The preparation method of the emasculation treatment solution in this embodiment is as follows:

[0088] Mix 3 parts ethephon, 3 parts gibberellin and 3 parts bensulfuron-methyl thoroughly, then add them to 7 parts conditioning solution and mix thoroughly to obtain the maserization treatment solution.

[0089] The preparation method of the conditioning liquid in this embodiment is as follows:

[0090] S101: Add graphene to a 6-fold solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed.

[0091] S102: Place the product of S01 in 4 times its volume of chitosan solution and stir thoroughly to obtain the conditioning solution.

[0092] The sodium lignosulfonate solution in this embodiment has a mass fraction of 15%.

[0093] In this embodiment, the ultrasonic dispersion power is 450W and the dispersion time is 40min.

[0094] The chitosan solution in this embodiment has a mass fraction of 5%.

[0095] Example 3.

[0096] The method for breeding hybrid glutinous sorghum according to this embodiment includes the following steps:

[0097] Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use;

[0098] Step 2, Pretreatment of sorghum plants:

[0099] Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags.

[0100] Step 3: Use bags containing the emasculation treatment solution to cover the pretreated sorghum plants and continue overnight treatment. Use japonica sorghum iron 239B as the female parent and glutinous sorghum maintainer line 72B as the male parent for hybridization. After self-pollination and selection of the hybrid offspring, a stable glutinous sorghum maintainer line B is bred, thus completing the breeding of hybrid glutinous sorghum.

[0101] The preparation method of the pretreatment agent in this embodiment is as follows:

[0102] S01: Improvement treatment of montmorillonite:

[0103] S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 5.5% of polyaspartic acid (total amount of zinc sulfate solution) and 3.5% of thermally modified montmorillonite (total amount of zinc sulfate solution), stir and mix thoroughly to obtain the pretreatment agent.

[0104] In this embodiment, the zinc sulfate solution has a mass fraction of 26% and the ferric sulfate solution has a mass fraction of 22.5%.

[0105] The specific operational steps for the montmorillonite conditioning and improvement treatment in this embodiment are as follows:

[0106] Montmorillonite was first heat-treated at 320℃ for 12.5 min, then cooled to 182℃ at a rate of 3℃ / min, held at that temperature for 7.5 min, and finally cooled to 60℃ at a rate of 2℃ / min.

[0107] Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried.

[0108] The sodium dodecylbenzenesulfonate solution in this embodiment has a mass fraction of 12.5%.

[0109] The preparation method of the emasculation treatment solution in this embodiment is as follows:

[0110] Mix 2 parts ethephon, 2 parts gibberellin and 2 parts bensulfuron-methyl thoroughly, then add to 5.5 parts conditioning solution and mix thoroughly to obtain the maserization treatment solution.

[0111] The preparation method of the conditioning liquid in this embodiment is as follows:

[0112] S101: Add graphene to a 4.5 times solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed.

[0113] S102: Place the product of S01 into 3 times the amount of chitosan solution and stir thoroughly to obtain the conditioning solution.

[0114] The sodium lignosulfonate solution in this embodiment has a mass fraction of 12.5%.

[0115] In this embodiment, the ultrasonic dispersion power is 400W and the dispersion time is 35min.

[0116] The chitosan solution in this embodiment has a mass fraction of 3.5%.

[0117] Example 4.

[0118] The method for breeding hybrid glutinous sorghum according to this embodiment includes the following steps:

[0119] Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use;

[0120] Step 2, Pretreatment of sorghum plants:

[0121] Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags.

[0122] Step 3: Use bags containing the emasculation treatment solution to cover the pretreated sorghum plants and continue overnight treatment. Use japonica sorghum iron 239B as the female parent and glutinous sorghum maintainer line 72B as the male parent for hybridization. After self-pollination and selection of the hybrid offspring, a stable glutinous sorghum maintainer line B is bred, thus completing the breeding of hybrid glutinous sorghum.

[0123] The preparation method of the pretreatment agent in this embodiment is as follows:

[0124] S01: Improvement treatment of montmorillonite:

[0125] S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 5% of polyaspartic acid (total of zinc sulfate solution) and 3% of thermally modified montmorillonite (total of zinc sulfate solution), stir and mix thoroughly to obtain the pretreatment agent.

[0126] In this embodiment, the zinc sulfate solution has a mass fraction of 25% and the ferric sulfate solution has a mass fraction of 22%.

[0127] The specific operational steps for the montmorillonite conditioning and improvement treatment in this embodiment are as follows:

[0128] Montmorillonite was first heat-treated at 312℃ for 12 minutes, then cooled to 182℃ at a rate of 3℃ / min, held for 6 minutes, and finally cooled to 60℃ at a rate of 2℃ / min.

[0129] Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried.

[0130] The sodium dodecylbenzenesulfonate solution in this embodiment has a mass fraction of 12%.

[0131] The preparation method of the emasculation treatment solution in this embodiment is as follows:

[0132] Mix 2 parts ethephon, 2 parts gibberellin and 2 parts bensulfuron-methyl thoroughly, then add to 5 parts conditioning solution and mix thoroughly to obtain the maleicide treatment solution.

[0133] The preparation method of the conditioning liquid in this embodiment is as follows:

[0134] S101: Add graphene to a 34-fold solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed.

[0135] S102: Place the product of S01 into 3 times the amount of chitosan solution and stir thoroughly to obtain the conditioning solution.

[0136] The sodium lignosulfonate solution in this embodiment has a mass fraction of 12%.

[0137] In this embodiment, the ultrasonic dispersion power is 360W and the dispersion time is 32min.

[0138] The chitosan solution in this embodiment has a mass fraction of 3%.

[0139] Example 5.

[0140] The method for breeding hybrid glutinous sorghum according to this embodiment includes the following steps:

[0141] Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use;

[0142] Step 2, Pretreatment of sorghum plants:

[0143] Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags.

[0144] Step 3: Use bags containing the emasculation treatment solution to cover the pretreated sorghum plants and continue overnight treatment. Use japonica sorghum iron 239B as the female parent and glutinous sorghum maintainer line 72B as the male parent for hybridization. After self-pollination and selection of the hybrid offspring, a stable glutinous sorghum maintainer line B is bred, thus completing the breeding of hybrid glutinous sorghum.

[0145] The preparation method of the pretreatment agent in this embodiment is as follows:

[0146] S01: Improvement treatment of montmorillonite:

[0147] S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 6% of polyaspartic acid (total amount of zinc sulfate solution) and 4% of thermally modified montmorillonite (total amount of zinc sulfate solution), stir and mix thoroughly to obtain the pretreatment agent.

[0148] In this embodiment, the zinc sulfate solution has a mass fraction of 26% and the ferric sulfate solution has a mass fraction of 24%.

[0149] The specific operational steps for the montmorillonite conditioning and improvement treatment in this embodiment are as follows:

[0150] Montmorillonite was first heat-treated at 325℃ for 14 minutes, then cooled to 184℃ at a rate of 3℃ / min, held at that temperature for 8 minutes, and finally cooled to 60℃ at a rate of 2℃ / min.

[0151] Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried.

[0152] The sodium dodecylbenzenesulfonate solution in this embodiment has a mass fraction of 14%.

[0153] The preparation method of the emasculation treatment solution in this embodiment is as follows:

[0154] Mix 2 parts ethephon, 2 parts gibberellin and 2 parts bensulfuron-methyl thoroughly, then add them to 6 parts conditioning solution and mix thoroughly to obtain the maserization treatment solution.

[0155] The preparation method of the conditioning liquid in this embodiment is as follows:

[0156] S101: Add graphene to a 5-fold solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed.

[0157] S102: Place the product of S01 into 3 times the amount of chitosan solution and stir thoroughly to obtain the conditioning solution.

[0158] The sodium lignosulfonate solution in this embodiment has a mass fraction of 10-15%.

[0159] In this embodiment, the ultrasonic dispersion power is 420W and the dispersion time is 38min.

[0160] The chitosan solution in this embodiment has a mass fraction of 4%.

[0161] Comparative Example 1.

[0162] Unlike Example 3, no pretreatment agent was used.

[0163] Comparative Example 2.

[0164] Unlike Example 3, the pretreatment agent did not contain thermally modified montmorillonite.

[0165] Comparative Example 3.

[0166] Unlike Example 3, the thermally improved montmorillonite is replaced with montmorillonite.

[0167] Comparative Example 4.

[0168] Unlike Example 3, no conditioning solution was added to the emasculation treatment solution.

[0169] Comparative Example 5.

[0170] Unlike Example 3, the conditioning solution was not treated with S101.

[0171] The performance measurement results of Examples 1-5 and Comparative Examples 1-5 are as follows:

[0172] Example 1 99.7 98 Example 2 99.8 99 Example 3 99.9 99 Example 4 99.8 98 Example 5 99.8 98 Comparative Example 1 93.5 94.2 Comparative Example 2 96.7 97.3 Comparative Example 3 97.2 96.5 Comparative Example 4 94.8 95.6 Comparative Example 5 96.5 97.8

[0173] From Examples 1-5 and Comparative Examples 1-5, it can be seen that the emasculation rate in Examples 1-5 of the present invention is as high as 99% or more, and the fruit setting rate is as high as 98% or more; the products of Examples 1-5 have excellent emasculation rate and fruit setting rate performance, and the performance of the prepared products can achieve coordinated improvement in emasculation rate and fruit setting rate performance.

[0174] In this invention, the performance of the product deteriorates significantly when no pretreatment agent is used or no conditioning solution is added to the emasculation treatment solution. The performance of the product also deteriorates when no thermally modified montmorillonite is added to the pretreatment agent, when montmorillonite is used instead of thermally modified montmorillonite, or when S101 treatment is not used in the conditioning solution. Only when the pretreatment agent and emasculation treatment solution of this invention are used can the performance of the product be significantly improved.

[0175] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within the present invention.

[0176] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A method for breeding hybrid glutinous sorghum, characterized in that, Includes the following steps: Step 1: Select sorghum plants that are growing well and in the flowering period, and set them aside for later use; Step 2, Pretreatment of sorghum plants: Spray the sorghum plants with a pretreatment agent, then bag them overnight, and remove the bags. Step 3: Use bags containing the emasculation treatment solution to cover the pretreated sorghum plants and continue overnight treatment. Use japonica sorghum iron 239B as the female parent and glutinous sorghum maintainer line 72B as the male parent for hybridization. After self-pollination and selection of the hybrid offspring, a stable glutinous sorghum maintainer line B is bred, thus completing the breeding of hybrid glutinous sorghum. The bag containing the virginity-reducing solution is formed by high-pressure injection of the virginity-reducing solution into the bag to create a mist-like solution; the preparation method of the pretreatment agent is as follows: S01: Improvement treatment of montmorillonite: S02: Mix zinc sulfate solution and ferric sulfate solution at a weight ratio of 2:1, then add 4-7% polyaspartic acid (total of zinc sulfate solution) and 2-5% thermally modified montmorillonite (total of zinc sulfate solution), and stir thoroughly to obtain a pretreatment agent; the mass fraction of the zinc sulfate solution is 24-28%; the mass fraction of the ferric sulfate solution is 20-25%; the specific operation steps for the montmorillonite conditioning and improvement treatment are as follows: Montmorillonite is first heat-treated at 310-330℃ for 10-15 minutes, then cooled to 180-185℃ at a rate of 2-4℃ / min, held for 5-10 minutes, and finally cooled to 60℃ at a rate of 1-3℃ / min. Montmorillonite at 60°C was immersed in a sodium dodecylbenzenesulfonate solution and stirred until thoroughly mixed. Finally, it was washed with water and dried. The mass fraction of the sodium dodecylbenzenesulfonate solution was 10-15%.

2. The method for breeding hybrid glutinous sorghum according to claim 1, characterized in that, The preparation method of the emasculation treatment solution is as follows: Mix 1-3 parts ethephon, 1-3 parts gibberellin and 1-3 parts benzsulfuron thoroughly, then add to 4-7 parts conditioning solution and mix thoroughly to obtain the maleicide treatment solution.

3. The method for breeding hybrid glutinous sorghum according to claim 2, characterized in that, The preparation method of the conditioning liquid is as follows: S101: Add graphene to a 3-6 times solution of sodium lignosulfonate, then add hydrochloric acid to adjust the pH to 5.5, and ultrasonically disperse until fully dispersed. S102: Place the product of S01 in 2-4 times its volume of chitosan solution and stir thoroughly to obtain the conditioning solution.

4. The method for breeding hybrid glutinous sorghum according to claim 3, characterized in that, The sodium lignosulfonate solution has a mass fraction of 10-15%.

5. The method for breeding hybrid glutinous sorghum according to claim 4, characterized in that, The ultrasonic dispersion power is 350-450W, and the dispersion time is 30-40min.

6. The method for breeding hybrid glutinous sorghum according to claim 5, characterized in that, The chitosan solution has a mass fraction of 2-5%.