A flavored xiaoyao wobei powder, a preparation method thereof and application thereof

Abstract

The present application relates to a kind of flavored Xiaoyao Fu Bei powder and its preparation method and application, the flavored Xiaoyao Fu Bei powder includes the following weight parts of raw materials: Chaihu 6-18 parts;When the 6-18 parts of Angelica;Fried melon 9-27 parts of Fu skin;Mountain 3-9 parts of Cigu;Milk bran fried 6-18 parts of Baishu;Zhebeimu 6-18 parts;Oyster 10-30 parts;Fried perilla 9-27 parts;White Fuling 6-18 parts;Prepared Banxia 6-18 parts;Fried melon 3-9 parts of Fuzi;Milk bran fried 6-18 parts of Baishao;Prepared Nansheng 6-18 parts;Fried wangbulu 9-27 parts;Prunella vulgaris 9-27 parts.Compared with prior art, the flavored Xiaoyao Fu Bei powder prepared by the present application is detected, and the main component is related to tumor microenvironment regulation, such as nocardone, oleanolic acid, solanum avicennifolium, peimine, etc., can significantly inhibit the growth of breast cancer, and has no damage to heart, liver, spleen, lung, kidney, intestinal tract.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine preparation technology, and in particular to a modified Xiaoyao Weibei powder, its preparation method and application. Background Technology

[0002] Triple-negative breast cancer (TNBC) is a subtype of breast cancer characterized by negative responses to estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor-2 (HER2). This means its cancer cells lack estrogen or progesterone receptors and have either no or low levels of HER2 receptors. It is a highly aggressive malignant tumor with poor treatment outcomes and a high risk of metastasis and recurrence. TNBC accounts for approximately 10%-20.8% of all breast cancers, and to some extent, it can be more invasive than hormone-positive or HER2-positive breast cancers.

[0003] Modern Traditional Chinese Medicine has made some progress in the treatment of TNBC. Xiaoyao Loubei Powder has been found to have a certain therapeutic effect on TNBC. The traditional Xiaoyao Loubei Powder generally consists of Bupleurum, Angelica sinensis, Paeonia lactiflora, Poria cocos, Trichosanthes kirilowii, Fritillaria cirrhosa, Pinellia ternata, Arisaema heterophyllum, raw oyster shell, and Cremastra appendiculata. To improve the efficacy of the drug, this study aims to create a modified Xiaoyao Weibei Powder based on the traditional Xiaoyao Loubei Powder. Summary of the Invention

[0004] The purpose of this invention is to provide a modified Xiaoyao Weibei Powder, its preparation method, and its application in order to improve the efficacy of the traditional Xiaoyao Loubei Powder.

[0005] The objective of this invention can be achieved through the following technical solutions:

[0006] One of the technical solutions of the present invention is to provide a modified Xiaoyao Weibei Powder, comprising the following raw materials in parts by weight: Bupleurum chinense 6-18 parts; Angelica sinensis 6-18 parts; stir-fried Trichosanthes kirilowii peel 9-27 parts; Cremastra appendiculata 3-9 parts; stir-fried Atractylodes macrocephala with honey bran 6-18 parts; Fritillaria thunbergii 6-18 parts; Ostrea gigas 10-30 parts; stir-fried Perilla frutescens seed 9-27 parts; Poria cocos 6-18 parts; processed Pinellia ternata 6-18 parts; stir-fried Trichosanthes kirilowii seed 3-9 parts; stir-fried Paeonia lactiflora with honey bran 6-18 parts; processed Arisaema heterophyllum 6-18 parts; stir-fried Vaccaria segetalis 9-27 parts; Prunella vulgaris 9-27 parts.

[0007] In some specific embodiments, the modified Xiaoyao Weibei Powder comprises the following ingredients in parts by weight: Bupleurum chinense 18 parts; Angelica sinensis 18 parts; stir-fried Trichosanthes kirilowii peel 27 parts; Cremastra appendiculata 9 parts; stir-fried Atractylodes macrocephala with honey bran 18 parts; Fritillaria thunbergii 18 parts; Ostrea gigas 30 parts; stir-fried Perilla frutescens seed 27 parts; Poria cocos 18 parts; processed Pinellia ternata 18 parts; stir-fried Trichosanthes kirilowii seed 9 parts; stir-fried Paeonia lactiflora with honey bran 18 parts; processed Arisaema heterophyllum 18 parts; stir-fried Vaccaria segetalis 27 parts; Prunella vulgaris 27 parts.

[0008] The second technical solution of the present invention is to provide a method for preparing Jiawei Xiaoyao Weibei Powder as described in one of the above technical solutions, comprising the following steps:

[0009] S1. Prepare each ingredient according to the following weight proportions: Bupleurum, Angelica sinensis, stir-fried Trichosanthes kirilowii peel, Cremastra appendiculata, stir-fried Atractylodes macrocephala with honey bran, Fritillaria thunbergii, Oyster shell, stir-fried Perilla frutescens seed, Poria cocos, processed Pinellia ternata, stir-fried Trichosanthes kirilowii seed, stir-fried Paeonia lactiflora with honey bran, processed Arisaema heterophyllum, stir-fried Vaccaria segetalis, and Prunella vulgaris.

[0010] S2. Submerge the raw materials from step S1 in water and decoct them to obtain a concentrated decoction, which is Jiawei Xiaoyao Weibei San.

[0011] In some specific implementations, in step S2, the concentrated decoction is specifically: the raw materials are submerged in water and decocted to a concentration of 8 times, that is, 8 bowls of water are decocted down to 1 bowl to obtain the decoction.

[0012] The third technical solution of the present invention is to provide an application of the modified Xiaoyao Weibei powder as described in one of the above technical solutions, wherein the modified Xiaoyao Weibei powder is used to prepare a drug for regulating the tumor microenvironment.

[0013] In some specific embodiments, the drug is a drug for treating breast cancer.

[0014] In some specific embodiments, the drug is a drug for treating triple-negative breast cancer.

[0015] In some specific embodiments, the drug is a drug that inhibits the growth of MCF-7 breast cancer cells.

[0016] In some specific embodiments, the drug is a drug that inhibits Ki67 expression in breast cancer cells.

[0017] In some specific embodiments, the dosage form of the drug includes liquid dosage form, gas dosage form, solid dosage form and semi-solid dosage form.

[0018] Compared with existing technologies, the Jiawei Xiaoyao Weibei Powder prepared by this invention, after testing, has main components such as nocarbone, oleanolic acid, solanine, and fritillary alkaloids that are related to the regulation of the tumor microenvironment. It can effectively regulate the tumor microenvironment and significantly inhibit the growth of breast cancer, and has no damaging effect on the heart, liver, spleen, lungs, kidneys, and intestines. Attached Figure Description

[0019] Figure 1 To analyze the components of Jiawei Xiaoyao Weibei Powder by mass spectrometry.

[0020] Figure 2 The effect of Jiawei Xiaoyao Weibei Powder on tumor growth.

[0021] Figure 3 To investigate the effect of Jiawei Xiaoyao Loubei Powder on the expression of Ki67 in tumors.

[0022] Figure 4 To investigate the toxic side effects of Jiawei Xiaoyao Loubei Powder in other parts of the mouse body. Detailed Implementation

[0023] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. These embodiments are based on the technical solution of the present invention and provide detailed implementation methods and specific operating procedures. However, the scope of protection of the present invention is not limited to the following embodiments.

[0024] Unless otherwise specified, the raw materials or processing techniques used in the following embodiments and comparative examples are all conventional commercially available raw materials or conventional processing techniques in the art.

[0025] This invention is a pure traditional Chinese medicine preparation, and all raw materials used are commonly used medicinal materials. It has no toxic side effects or adverse reactions. Unless otherwise stated, the main terms in this specification are defined as follows:

[0026] (1) Bupleurum is the root of Bupleurum chinense and Bupleurum stenoptera, plants of the Apiaceae family. It tastes bitter and is slightly cold in nature. It enters the liver and gallbladder meridians. It has the effects of harmonizing the exterior and interior, soothing the liver and relieving depression, raising yang and lifting prolapse, and reducing fever and stopping malaria.

[0027] (2) Angelica sinensis, also known as dried angelica, horse-tail angelica, Qinna, horse-tail angelica, cloud angelica, western angelica, and the dream of Min angelica, is a medicinal part of the root of Angelica sinensis, a plant in the Apiaceae family. It has a sweet and pungent taste and is warm in nature. It enters the liver, heart, and spleen meridians. It has the effects of nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and promoting bowel movements.

[0028] (3) Trichosanthes peel is the peel of Trichosanthes kirilowii, a perennial herbaceous vine belonging to the Cucurbitaceae family. It is sweet in taste and cold in nature. It enters the lung and stomach meridians. It has the effects of regulating qi and relieving bloating, resolving phlegm and relieving numbness, and clearing the lungs and relieving cough.

[0029] The stir-fried Trichosanthes peel used in this invention is a processed medicinal material of Trichosanthes peel. Compared with Trichosanthes peel, stir-fried Trichosanthes peel has a weaker cold nature, a slightly charred aroma, and is better at relieving chest tightness and promoting qi circulation.

[0030] (4) *Cremastra appendiculata* (D.Don) Makino, *Pleione bulbocodioides* (Franch.) Rolfe, or *Pleione yunnanensis* Rolfe are all orchid species. They have a sweet and slightly pungent taste and are cool in nature. They possess the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules.

[0031] (5) Atractylodes macrocephala, also known as Atractylodes macrocephala, is the dried rhizome of the plant Atractylodes macrocephala in the Asteraceae family. It has a bitter and sweet taste and is warm in nature. It enters the spleen and stomach meridians. It has the effects of tonifying the spleen, benefiting the stomach, drying dampness, harmonizing the middle jiao, and calming the fetus.

[0032] This invention uses honey-braised Atractylodes macrocephala as a processed medicinal material. Compared with Atractylodes macrocephala, honey-braised Atractylodes macrocephala has a stronger drying effect.

[0033] (6) Fritillaria thunbergii, the bulb of Fritillaria thunbergii, a plant of the Liliaceae family, is bitter and cold in nature. It enters the lung and heart meridians. It has the effects of clearing heat, resolving phlegm, relieving cough, detoxifying, dispersing nodules, and eliminating carbuncles.

[0034] (7) Oyster, the shell of oysters such as the Pacific oyster, the long oyster, or the Dalian Bay oyster. It is salty and moist in taste, and cool in nature. It enters the liver and kidney meridians. It has the effects of nourishing yin and suppressing yang, softening hardness and dissipating nodules, and astringing.

[0035] (8) Perilla seed, the fruit of Perilla frutescens and Perilla frutescens, both belonging to the Lamiaceae family. It has a pungent taste and warm properties. It enters the Lung meridian. It is used to treat cough and asthma, phlegm accumulation and shortness of breath, and constipation due to intestinal dryness.

[0036] This invention uses roasted perilla seeds, which are processed medicinal materials of perilla seeds. Compared with perilla seeds, roasted perilla seeds have a milder medicinal effect.

[0037] (9) White Poria cocos is the dried sclerotium of Poria cocos, a fungus belonging to the Polyporaceae family. It has a sweet and bland taste and a neutral nature. It enters the Heart, Spleen, Lung, and Kidney meridians. It is good at draining dampness and promoting urination, and it can also strengthen the spleen and calm the mind.

[0038] (10) Pinellia ternata is the tuber of Pinellia ternata, a plant in the Araceae family. It is pungent and warm in nature and is poisonous. It enters the spleen, stomach, and lung meridians. It is used for cough and asthma with excessive phlegm, dizziness and palpitations due to phlegm retention, dizziness due to internal phlegm, vomiting and nausea, chest and epigastric fullness, and globus hystericus.

[0039] This invention uses processed Pinellia ternata, which is a processed medicinal material of Pinellia ternata. Compared with Pinellia ternata, processed Pinellia ternata has eliminated toxicity and has better medicinal properties and efficacy.

[0040] (11) Trichosanthes seeds are the dried, mature seeds of Trichosanthes kirilowii Maxim. or Trichosanthes rosthornii Harms, both belonging to the Cucurbitaceae family. They are sweet in taste and cold in nature. They enter the Lung, Stomach, and Large Intestine meridians. They are used to treat cough with phlegm and heat, constipation, carbuncles, and insufficient lactation.

[0041] This invention uses roasted Trichosanthes kirilowii seeds, which are processed medicinal materials of Trichosanthes kirilowii seeds. Compared with Trichosanthes kirilowii seeds, roasted Trichosanthes kirilowii seeds have a weaker cold nature.

[0042] (12) White peony root (scientific name: Paeonia lactiflora Pall.), also known as white-flowered peony, is a plant belonging to the genus Paeonia in the family Ranunculaceae. The medicinal part is the root of the white peony root. It tastes bitter and sour, and is slightly cold in nature. It enters the liver and spleen meridians. It has the effects of nourishing blood and softening the liver, relieving pain, and astringing yin and stopping sweating.

[0043] This invention uses honey-braised white peony root, which is a processed medicinal material of white peony root. Compared with white peony root, honey-braised white peony root has a slightly milder medicinal effect and mainly nourishes blood and astringes yin.

[0044] (13) Arisaema, the tuber of Arisaema heterophyllum, Arisaema davidii, or Arisaema heterophyllum, all belonging to the Araceae family. It has a bitter and pungent taste, is warm in nature, and is poisonous. It enters the lung, liver, and spleen meridians. It is used for stubborn cough with phlegm, dizziness due to wind-phlegm, phlegm obstruction due to stroke, facial paralysis, hemiplegia, epilepsy, infantile convulsion, and tetanus.

[0045] This invention uses processed Arisaema heterophyllum, which is a medicinal material prepared from Arisaema heterophyllum. Compared with Arisaema heterophyllum, processed Arisaema heterophyllum has reduced toxicity and better medicinal properties and efficacy.

[0046] (14) Wang Bu Liu Xing is the dried, mature seed of Vaccaria segetalis (Neck.) Garcke, a plant in the Caryophyllaceae family. It has a bitter taste and neutral properties. It enters the liver and stomach meridians. It has the effects of promoting blood circulation and regulating menstruation, promoting lactation and reducing swelling, and promoting urination and relieving strangury.

[0047] This invention uses stir-fried Vaccaria segetalis, which is a processed medicinal material of Vaccaria segetalis. Compared with Vaccaria segetalis, stir-fried Vaccaria segetalis is more likely to exert its effects.

[0048] (15) Prunella vulgaris L., a plant of the Lamiaceae family, is a dried fruit spike with a pungent and bitter taste and a cold nature. It enters the liver and gallbladder meridians. It has the effects of clearing heat, improving eyesight, dispersing nodules, and reducing swelling.

[0049] All of the processed medicinal materials were prepared using conventional processing methods.

[0050] Example 1:

[0051] This embodiment 1 provides a decoction of modified Xiaoyao Weibei Powder, the preparation method of which includes the following steps:

[0052] Place 18g Bupleurum, 18g Angelica sinensis, 27g stir-fried Trichosanthes kirilowii peel, 9g Cremastra appendiculata, 18g stir-fried Paeonia lactiflora with honey, 18g Fritillaria thunbergii, 30g Ostrea gigas, 27g stir-fried Perilla frutescens, 18g Poria cocos, 18g processed Pinellia ternata, 9g stir-fried Trichosanthes kirilowii, 18g stir-fried Paeonia lactiflora with honey, 18g processed Arisaema heterophyllum, 27g stir-fried Vaccaria segetalis, and 27g Prunella vulgaris in a pot, cover with water, and decoct to a concentration 8 times. The resulting decoction is the modified Xiaoyao Weibei Powder Decoction.

[0053] Mass spectrometry was used to analyze the active ingredients in Jiawei Xiaoyao Weibei San decoction, such as... Figure 1 As shown in Figure A, nootkatone can be seen in the figure. Figure 1 B) Oleanolic acid Figure 1 C) Solasodine (a type of lycopene) Figure 1D) Peimine ( Figure 1 The content of E) and other active ingredients is relatively high, and according to reports, these active ingredients are related to the regulation of the tumor microenvironment.

[0054] Comparative Example 1:

[0055] This comparative example provides a traditional Xiaoyao Loubei powder decoction, the preparation method of which includes the following steps:

[0056] Place 18g Bupleurum, 18g Angelica sinensis, 18g Paeonia lactiflora, 18g Poria cocos, 9g Trichosanthes kirilowii, 18g Fritillaria cirrhosa, 18g Pinellia ternata, 18g Arisaema heterophyllum, 30g raw oyster shell, and 9g Cremastra appendiculata in a pot, cover with water, and decoct to a concentration 8 times. The resulting decoction is the traditional Xiaoyao Loubei San decoction.

[0057] The modified Xiaoyao Weibei powder decoction prepared in Example 1 and the traditional Xiaoyao Loubei powder decoction prepared in Comparative Example 1 were subjected to the following in vivo mouse experiments:

[0058] (1) Construction of a subcutaneous tumor model of breast cancer

[0059] MCF-7 cells were cultured in carbon dioxide medium at 37°C until they reached 80%-90% confluence. The medium was discarded, and the cells were washed with PBS buffer, then digested with trypsin for 1 min. Finally, DMEM complete medium was added to terminate the digestion. The culture was centrifuged at 1000 rpm for 4 min to collect the cells, and the cell concentration was adjusted to 1×10⁶ cells / mL with PBS buffer. 6 A tumor-bearing mouse model was established by subcutaneously injecting 1 mL of cells into BALB / c immunodeficient mice.

[0060] Treatment group 1: 1 mL of traditional Xiaoyao Loubei San decoction was administered by gavage daily; Treatment group 2: 1 mL of modified Xiaoyao Loubei San decoction was administered by gavage daily; Control group: an equal amount of physiological saline was administered by gavage daily.

[0061] Starting from day 5 after cell injection, the tumor volume was measured using calipers. On day 30, the mice were sacrificed, and the tumors were removed for volume and weight measurements.

[0062] The results are as follows Figure 2 As shown, the tumor cell volume (***P<0.001) and weight (***P<0.001) in treatment group 2 were significantly smaller than those in treatment group 1 and the control group, indicating that the modified Xiaoyao Loubei powder decoction inhibited the growth of MCF-7 breast cancer cells after oral administration. Figure 2 A), including the volume of tumor cells ( Figure 2 B) and weight ( Figure 2 C).

[0063] (2) Immunohistochemical detection of Ki67 expression

[0064] Fixation: First, fix the tumor tissue sample taken out in the fixative solution (4% paraformaldehyde) for more than 24 hours.

[0065] Dehydration: First, clean the fixed tumor tissue sample with deionized water, and then wash it with 70% alcohol, 80% alcohol, 90% alcohol, 95% alcohol, 100% alcohol, 100% alcohol and 100% alcohol for 1 hour each. The 70% alcohol can be left overnight.

[0066] Transparency: The dehydrated tumor tissue sample was immersed in a mixture of xylene and alcohol at a volume ratio of 1:1 (20 times the volume) until the tumor tissue sample became transparent. Then, it was immersed in pure xylene for 10 minutes, and the immersion in pure xylene was repeated for 10 minutes.

[0067] Paraffin infiltration: The cleared tumor tissue sample was infiltrated with paraffin in a constant temperature oven at 60°C for 3 hours, and then placed in an embedding cassette and cooled on a cold stage.

[0068] Sectioning: The paraffin-impregnated tumor tissue sample was trimmed and sectioned to a thickness of 5 μm.

[0069] Slicing: Use a spatula to remove the spread paraffin slices and place them on a baking machine. Dry the numbered paraffin slices overnight in an oven at 30-40℃.

[0070] Dewaxing and hydrophilicization: The dried paraffin slices were immersed in xylene for dewaxing for 5 minutes each time, and repeated 4 times. Then, they were successively immersed in 100% alcohol, 100% alcohol, 95% alcohol, 90% alcohol, 80% alcohol, 70% alcohol, deionized water, deionized water and deionized water, respectively, for 5 minutes each time.

[0071] Staining: After dewaxing and hydrophilic treatment, tumor tissue samples were immersed in 0.3% hydrogen peroxide at room temperature for 15 minutes to remove endogenous peroxidase. Then, 0.4% pepsin (prepared with 10mM Tris-HCl, pH 2.0) was added and the samples were treated at 37°C for 20 minutes. Sheep serum blocking solution was added and the samples were treated at 37°C for 1 hour. After blotting the blocking solution around the tumor tissue samples with absorbent paper, rabbit anti-human Ki67 antibody (1% BSA diluted in PBS, 1:500 volume dilution) was added and the samples were incubated overnight at 4°C. After washing three times with TBST (TBS buffer containing 0.025% Tritonx-100), mouse anti-human secondary antibody (1% BSA diluted in PBS, 1:500 volume dilution) was added and the samples were incubated at 37°C for 1 hour. After washing three times with Tris-buffered saline (TBST: TBS buffer containing 0.025% Tritonx-100), add DAB chromogenic solution and treat at 37°C for 10 minutes. Stop treatment after complete chromogenic development. Rinse the developed slides with water and counterstain with hematoxylin for 20 minutes. After soaking in deionized water, soak in 70% alcohol, 80% alcohol, 90% alcohol, 95% alcohol, 100% alcohol and 100% alcohol for 5 minutes each. Then soak in xylene four times for 5 minutes each time.

[0072] Resin mounting: The sections must not be dried before mounting. Dip a small amount of xylene into the end of the coverslip and drop a drop of neutral resin in the middle of the sample. Be careful not to have air bubbles when mounting.

[0073] Microscopic observation: After the resin dries, observe it under a microscope. The results are as follows. Figure 3 As shown, compared with the saline control group and the traditional Xiaoyao Loubei powder decoction treatment group 1, the modified Xiaoyao Loubei powder decoction can better inhibit the expression of Ki67 in breast cancer tumor tissue after gavage.

[0074] (3) Effects of immunohistochemical detection on toxic side effects in other parts of mice

[0075] Tissues from other parts of the mice treated in experiment (1) were taken out and stained for observation.

[0076] The specific steps are as follows:

[0077] Fixation: First, fix the tissue samples from the above sites with fixative (4% paraformaldehyde) for more than 24 hours.

[0078] Dehydration: First, clean the fixed tissue sample with deionized water, then wash it with 70% alcohol, 80% alcohol, 90% alcohol, 95% alcohol, 100% alcohol, 100% alcohol and 100% alcohol for 1 hour each, and the 70% alcohol can be left overnight.

[0079] Transparency: Immerse the dehydrated tissue sample in a 20-fold volume mixture of xylene and alcohol (xylene to alcohol volume ratio of 1:1) until the tissue sample becomes transparent. Then, transfer it to pure xylene and immerse it for 10 minutes. Repeat the immersion in pure xylene for 10 minutes.

[0080] Paraffin infiltration: The cleared tissue sample is infiltrated with paraffin at 60°C for 3 hours, then placed in an embedding cassette and cooled on a cold stage.

[0081] Sectioning: The paraffin-impregnated tissue samples are trimmed and sectioned to a thickness of 5 μm.

[0082] Slicing: Use a spatula to remove the spread paraffin slices and place them on a baking machine. Dry the numbered paraffin slices overnight in an oven at 30-40℃.

[0083] Dewaxing paraffin sections to water: Place the sections in xylene I for 20 min, xylene II for 20 min, anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, and 75% ethanol for 5 min, then wash with tap water.

[0084] Hematoxylin staining: Immerse the sections in hematoxylin staining solution for 3-5 minutes, wash with tap water, differentiate with differentiation solution, wash with tap water, blue with blueing solution, and rinse with running water.

[0085] Eosin staining: The sections were dehydrated in 85% and 95% graded alcohol solutions for 5 min each, and then stained in eosin staining solution for 5 min.

[0086] Dehydration and mounting: The sections were sequentially immersed in anhydrous ethanol I for 5 min, anhydrous ethanol II for 5 min, anhydrous ethanol III for 5 min, xylene I for 5 min, and xylene II for 5 min. After clearing, the sections were mounted with neutral resin.

[0087] Microscopic examination, image acquisition and analysis. Results are as follows: Figure 4 As shown, HE staining confirmed that neither the modified Xiaoyao Loubei San decoction nor the traditional Xiaoyao Loubei San decoction had any toxic side effects on the heart, liver, spleen, lungs, kidneys, or intestines after oral administration. This indicates that the modified Xiaoyao Loubei San decoction prepared in this invention can be used as a good traditional Chinese medicine compound for preparing anti-tumor and microenvironment-improving treatments.

[0088] The above description of the embodiments is provided to enable those skilled in the art to understand and use the invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments, and any improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the invention should be within the protection scope of the present invention.

Claims

1. A modified Xiaoyao Weibei powder for treating breast cancer, characterized in that, The modified Xiaoyao Weibei powder is used to regulate the tumor microenvironment. The modified Xiaoyao Weibei powder is made from the following raw materials in parts by weight: Bupleurum chinense 18 parts; Angelica sinensis 18 parts; stir-fried Trichosanthes kirilowii peel 27 parts; Cremastra appendiculata 9 parts; stir-fried Atractylodes macrocephala with honey bran 18 parts; Fritillaria thunbergii 18 parts; Ostrea gigas 30 parts; stir-fried Perilla frutescens seed 27 parts; Poria cocos 18 parts; processed Pinellia ternata 18 parts; stir-fried Trichosanthes kirilowii seed 9 parts; stir-fried Paeonia lactiflora with honey bran 18 parts; processed Arisaema heterophyllum 18 parts; stir-fried Vaccaria segetalis 27 parts; Prunella vulgaris 27 parts.

2. A method for preparing the flavored Xiaoyao Weibei powder as described in claim 1, characterized in that, Includes the following steps: S1. Prepare each ingredient according to the following weight proportions: Bupleurum, Angelica sinensis, stir-fried Trichosanthes kirilowii peel, Cremastra appendiculata, stir-fried Atractylodes macrocephala with honey bran, Fritillaria thunbergii, Oyster shell, stir-fried Perilla frutescens seed, Poria cocos, processed Pinellia ternata, stir-fried Trichosanthes kirilowii seed, stir-fried Paeonia lactiflora with honey bran, processed Arisaema heterophyllum, stir-fried Vaccaria segetalis, and Prunella vulgaris. S2. Submerge the raw materials from step S1 in water and decoct them to obtain a concentrated decoction, which is the flavored Xiaoyao Weibei Powder decoction.

3. The preparation method of Jiawei Xiaoyao Weibei Powder according to claim 2, characterized in that, In step S2, the concentrated decoction is specifically: the raw materials are submerged in water and decocted to a concentration of 8 times, that is, 8 bowls of water are decocted down to 1 bowl to obtain the decoction.

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