A method for producing probiotic nutrient feed using distiller's yellow water

Abstract

The application discloses a method for producing probiotic nutrient feed by using yellow water for brewing liquor, and comprises the following steps: firstly, removing impurities from fresh yellow water to obtain yellow water concentrated nutrient solution; then, taking the yellow water concentrated nutrient solution as a basic medium, and inoculating yeast to obtain a yeast suspension; subsequently, inoculating the obtained yeast suspension into bran for culture and drying to obtain nutrient bran; and then, mixing the nutrient bran with the yellow water concentrated nutrient solution according to a proportion, drying and granulating to obtain probiotic nutrient feed. The probiotic nutrient feed obtained by the method has high contents of crude protein, peptides and lactic acid, complete amino acid components, and contains probiotic yeast, so that the probiotic nutrient feed can promote the digestion and metabolism of livestock and improve the immunity of the livestock.

Description

Technical Field

[0001] This invention belongs to the field of animal feed production, specifically relating to a method for producing probiotic nutritional feed using brewing liquid. Background Technology

[0002] The brewing process of strong-aroma baijiu is a traditional solid-state fermentation, which produces a large amount of yellow water. This is mainly because the brewing process only utilizes the sugars and starches in the raw materials, while other substances such as cellulose, proteins, and phenols dissolve in the fermentation mash and directly settle to the bottom of the fermentation tank. These substances, along with the liquid produced by fermentation metabolism and the moisture in the mash itself, form the yellow water produced in traditional brewing. Therefore, the concentration of organic matter in the yellow water, a byproduct of strong-aroma baijiu brewing, is relatively high. Typically, yellow water contains 1-2% residual starch, 0.3-0.7% residual sugar, 4-5% ethanol, as well as a large amount of organic acids, alcohols, aldehydes, antioxidants, humic substances, autolysates of yeast cells, microbial cells, and live cells, making it highly valuable. Lactic acid accounts for over 70% of the organic matter in the yellow water, and Bacillus bacteria, primarily lactic acid bacteria, constitute a significant portion, laying the foundation for the use of yellow water as animal feed.

[0003] For example, CN111436536A discloses a method for the resource utilization of brewing by-product yellow water. Using yellow water as a raw material, the pH value is adjusted and the water is directly concentrated. A food-grade anti-sticking agent is added, and the mixture is dried and pulverized to obtain animal feed. The nutritional content of the animal feed obtained by this method is limited to the inherent nutrients in the brewing by-product yellow water, mainly lactic acid, with limited protein content.

[0004] For example, CN113545418A discloses the application of yellow water in ruminant feeding, directly replacing energy feeds such as corn and protein feeds such as soybean meal in ruminant concentrate feed to achieve a reduction in the amount of yellow water used in ruminant concentrate feed. However, this method of directly feeding ruminants with yellow water has poor palatability, involves the transfer and storage of yellow water, and has a limited feeding volume, thus failing to fundamentally solve the problem of yellow water's application in feed.

[0005] The two methods of using yellow water as feed mentioned above have limited nutritional value and low added value from the utilization of yellow water resources, making them unsuitable for large-scale, industrialized applications. In addition, they lack probiotics, and their effect on regulating the animal's gastrointestinal tract by relying solely on the lactic acid from yellow water is limited. Summary of the Invention

[0006] This invention addresses the shortcomings of existing technologies by providing a method for producing probiotic nutritional feed using brewing wastewater. The probiotic nutritional feed obtained by this invention is high in crude protein, peptides, and lactic acid, contains a complete amino acid profile, and includes beneficial yeast, which can promote digestion and metabolism in livestock and enhance their immunity.

[0007] The present invention discloses a method for producing probiotic nutritional feed using brewing wastewater, comprising the following steps:

[0008] Step 1: Preparation of concentrated nutrient solution from yellow water

[0009] Take fresh yellow water, filter to remove impurities, use a 2:1 ratio of food-grade or feed-grade sodium hydroxide and calcium hydroxide as pH adjusters to adjust the pH to 5.5±0.2, and then use a multi-effect evaporator to concentrate the yellow water to 1 / 3 to 1 / 5 of the original volume to obtain concentrated yellow water nutrient solution; the solid content of the concentrated yellow water nutrient solution is 30-50%.

[0010] Step 2: Preparation of yeast suspension

[0011] Using water as a medium, add concentrated nutrient solution of yellow water, yeast extract and glucose, and adjust the pH to 5.6-6.5 with food-grade or feed-grade sodium hydroxide and calcium hydroxide in a 2:1 ratio, sterilize at 115℃ for 30 min to obtain yeast culture medium;

[0012] Based on water content, the addition amount of concentrated nutrient solution in yellow water is 0.5–2 wt%, the addition amount of yeast extract is 0.1–0.5 g / 100 mL, and the addition amount of glucose is 0.5–1 g / 100 mL.

[0013] The first and second yeast strains were inoculated into yeast culture medium and cultured at 28–30°C for 24–36 hours, yielding 10⁶ cells / cells each. 9 -10 10 First yeast suspension and second yeast suspension, CFU / mL.

[0014] The first yeast strain is *Saccharomyces cerevisiae* GJYD02, which has been deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 2017132, deposit date March 20, 2017, address: Wuhan University, Wuhan, China. *Saccharomyces cerevisiae* GJYD02 exhibits heat and acid resistance, tolerating temperatures up to 45℃ and an acid tolerance range of pH 2.0-9.0. The total protein content of the cells is 48.98%.

[0015] The second yeast strain is *Candida tropicalis* GJY125, which has been deposited at the China Center for Type Culture Collection (CCTCC), accession number CCTCC NO: M 20221369, deposit date September 5, 2022, address: Wuhan University, Wuhan, China. The total protein content of the cells is 55.31%.

[0016] Step 3: Preparation of No. 1 Nutrient Bran

[0017] Mix 99 wt% wheat bran and 1 wt% concentrated nutrient solution of yellow water according to mass percentage, add water to a moisture content of 50%-60%, and sterilize to obtain the fermentation raw material; inoculate the obtained fermentation raw material with 10 wt% of the first yeast suspension, and culture aerobically at 28-35℃ for 24-36 hours, until the number of brewer's yeast in the wheat bran is >6*10. 9 CFU / g, dried at 45℃ to obtain No. 1 nutrient bran;

[0018] Step 4: Preparation of No. 2 Nutrient Bran

[0019] Mix 97 wt% wheat bran, 2 wt% urea, and 1 wt% concentrated nutrient solution of yellow water according to mass percentage, add water to a moisture content of 50%-60%, and sterilize to obtain the fermentation raw material; inoculate the obtained fermentation raw material with a second yeast suspension at 10 wt%, and culture aerobically at 28-35℃ for 24-36 hours until the number of Candida tropicalis in the wheat bran is >10. 9 CFU / g, dried at 80℃, to obtain No. 2 nutrient bran;

[0020] Step 5: Preparation of Probiotic Nutritional Feed

[0021] Yellow water concentrated nutrient solution, No. 1 nutrient bran, and No. 2 nutrient bran were mixed in a mass ratio of 4:3:3, dried at 45℃, and granulated to obtain a probiotic nutrient feed with a brewer's yeast viable count >10. 9 CFU / g, total peptides ≥15%, crude protein ≥25%, lactic acid ≥70000mg / kg, and 17 kinds of amino acids.

[0022] Compared with the prior art, the beneficial effects of the present invention are reflected in:

[0023] The production method of this invention for probiotic nutritional feed increases the total protein and total peptide content of the feed while fully preserving the beneficial components in the yellow water. Furthermore, the nutritional feed obtained by this invention contains a high number of live Saccharomyces cerevisiae, which can improve the microecological balance of the animal's digestive tract. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this invention clear and complete, the embodiments of this invention are further described in detail below. It should be understood that the specific embodiments described herein are only some, not all, embodiments of this invention, and are merely illustrative of the embodiments of this invention. They are not intended to limit the embodiments of this invention. All other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this invention.

[0025] Example 1:

[0026] Take fresh yellow water, filter to remove impurities, adjust the pH to 5.5 using a 2:1 ratio of food-grade or feed-grade sodium hydroxide and calcium hydroxide, and concentrate the yellow water to 1 / 5 of its original volume in a multi-effect evaporator to obtain concentrated yellow water nutrient solution.

[0027] Using water as the medium, concentrated yellow water nutrient solution, yeast extract, and glucose were added. The pH was adjusted to 6.5 using a 2:1 ratio of food-grade or feed-grade sodium hydroxide and calcium hydroxide. The mixture was then sterilized at 115℃ for 30 minutes to obtain the yeast culture medium. The amount of concentrated yellow water nutrient solution added was 1 wt%, the amount of yeast extract added was 0.3 g / 100 mL, and the amount of glucose added was 1 g / 100 mL.

[0028] Yeast GJYD02 and Candida tropicalis GJY125 were inoculated into yeast culture medium and cultured at 28℃ for 36 h. The resulting yeast suspensions had a cell count of 2.5 × 10⁻⁶. 10 CFU / mL, and the second yeast suspension, with a cell count of 1.5*10⁻⁶. 10 CFU / mL.

[0029] Using 99 wt% wheat bran and 1 wt% concentrated yellow water nutrient solution, mixed with water to 50% moisture content and sterilized, as fermentation raw materials, a first yeast suspension was inoculated at 10 wt% and cultured aerobically at 28℃ for 24-36 hours. The wheat bran yeast count was 9.3*10^9. 9 CFU / g, dried at 45℃ to obtain No. 1 nutrient bran.

[0030] Using a concentrated nutrient solution of 97 wt% wheat bran, 2 wt% urea, and 1 wt% yellow water, mixed with water to a moisture content of 50% and sterilized, as the fermentation raw material, a second yeast suspension was inoculated at 10 wt% and cultured aerobically at 30°C for 36 hours. The wheat bran yeast count was 4.5 x 10^6 cells / day. 9 CFU / g, dried at 80℃, to obtain No. 2 nutrient bran.

[0031] Mix the concentrated nutrient solution, No. 1 nutrient bran, and No. 2 nutrient bran in a mass ratio of 4:3:3, dry at a low temperature of 45℃, and granulate to obtain probiotic nutrient feed.

[0032] Example 2:

[0033] Using yellow water as raw material, the pH value is adjusted to 5.5 by using food-grade or feed-grade sodium hydroxide and calcium hydroxide in a 2:1 ratio. The mixture is then concentrated to 1 / 5 of its original volume in a multi-effect evaporator. It is then mixed with wheat bran in a 4:6 ratio, dried at 80℃, and pulverized to obtain animal feed.

[0034] Example 3:

[0035] Based on Example 1, 97 wt% wheat bran, 2 wt% urea, and 1 wt% concentrated nutrient solution were mixed by weight percentage, and water was added to a moisture content of 50%. After sterilization, the fermentation raw material was obtained. The first yeast suspension and the second yeast suspension were mixed evenly at a 1:1 ratio, and then inoculated into the obtained fermentation raw material at 10 wt%. The mixture was aerobically cultured at 30°C for 36 hours, and the yeast count in the wheat bran was 1.8 x 10^6 cells / day. 9 CFU / g was dried at a low temperature of 45℃ to obtain nutritious bran.

[0036] The concentrated nutrient solution prepared according to the method of Example 1 was mixed with the above-mentioned nutrient bran at a mass ratio of 4:6, dried at a low temperature of 45°C, and granulated to obtain nutrient feed.

[0037] Example 4:

[0038] Based on Example 1, no concentrated nutrient solution was added to the fermentation raw materials of both No. 1 and No. 2 nutrient bran. The cultivation conditions and drying temperature were the same as in Example 1. The yeast count in the obtained No. 1 nutrient bran was 9*10. 8 CFU / g, No. 2 nutrient bran yeast count 1.5*10 9 CFU / g.

[0039] Mix the concentrated nutrient solution, No. 1 nutrient bran, and No. 2 nutrient bran in a mass ratio of 4:3:3, dry at a low temperature of 45℃, and granulate to obtain probiotic nutrient feed.

[0040] Table 1. Nutritional indicators of feed in each embodiment

[0041]

[0042]

[0043] As shown in Table 1, compared with the other three examples, Example 1 showed a significant increase in the content of crude protein, total peptides, crude fat, and amino acids, especially in crude protein, total peptides, and amino acids. Furthermore, the yeast viable count in the feed of Example 1 was 2.5 x 10⁻⁶. 9 CFU / g, compared to 3*10^3 live yeast cells in the feed of Examples 3 and 4. 8 CFU / g and 8*10 7 The CFU / g ratio was increased by one to two orders of magnitude. This indicates that the nutritional feed of Example 1 had a higher nutritional content and better probiotic function.

Claims

1. A method for producing probiotic nutritional feed using brewing wastewater, characterized in that... Includes the following steps: Step 1: Preparation of concentrated nutrient solution from yellow water Take fresh yellow water, filter to remove impurities, adjust the pH to 5.5±0.2 using a pH adjuster, and then concentrate the yellow water to 1 / 3 to 1 / 5 of its original volume using a multi-effect evaporator to obtain concentrated yellow water nutrient solution. Step 2: Preparation of yeast suspension Using water as a medium, add concentrated nutrient solution of yellow water, yeast extract and glucose, adjust the pH to 5.6-6.5 with a pH adjuster, and sterilize at 115℃ for 30 min to obtain yeast culture medium; The first and second yeast strains were inoculated into yeast culture medium and cultured at 28–30°C for 24–36 hours, yielding 10⁶ cells / cells each. 9 -10 10 First yeast suspension and second yeast suspension, CFU / mL; The first yeast strain is Saccharomyces cerevisiae GJYD02, preservation number CCTCC NO: M 2017132; The second yeast strain is Candida tropicalis GJY125, preservation number CCTCC NO: M 20221369; Step 3: Preparation of No. 1 Nutrient Bran Mix 99 wt% wheat bran and 1 wt% concentrated nutrient solution of yellow water according to mass percentage, add water to a moisture content of 50%-60%, and sterilize to obtain the fermentation raw material; inoculate the obtained fermentation raw material with a first yeast suspension at a volume of 10 wt%, and culture aerobically at 28-35℃ for 24-36 hours until the yeast count in the wheat bran is >6×10⁻⁶. 9 CFU / g, dried at 45℃ to obtain No. 1 nutrient bran; Step 4: Preparation of No. 2 Nutrient Bran Mix 97wt% wheat bran, 2wt% urea, and 1wt% concentrated nutrient solution of yellow water according to the mass percentage, add water to a moisture content of 50%-60%, and sterilize to obtain the fermentation raw material; inoculate the obtained fermentation raw material with a second yeast suspension at a volume of 10wt%, and culture aerobically at 28-35℃ for 24-36 hours until the yeast count in the wheat bran is >10. 9 CFU / g, dried at 80℃, to obtain No. 2 nutrient bran; Step 5: Preparation of Probiotic Nutritional Feed The concentrated nutrient solution from the yellow water, No. 1 nutrient bran, and No. 2 nutrient bran were mixed, dried at 45℃, and granulated to obtain a probiotic nutrient feed with a brewer's yeast viable count >10. 9 CFU / g, total peptides ≥15%, crude protein ≥25%, lactic acid ≥70000mg / kg, and 17 kinds of amino acids.

2. The method according to claim 1, characterized in that: The pH adjuster is composed of food-grade or feed-grade sodium hydroxide and calcium hydroxide in a mass ratio of 2:

1.

3. The method according to claim 1, characterized in that: In step 2, the amount of concentrated nutrient solution added is 0.5~2wt% based on water, the amount of yeast extract added is 0.1~0.5g / 100mL, and the amount of glucose added is 0.5~1g / 100mL.

4. The method according to claim 1, characterized in that: In step 5, the mass ratio of the concentrated nutrient solution, No. 1 nutrient bran, and No. 2 nutrient bran is 4:3:3.

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