Sample loading and receiving device for protein ultrafiltration process and application method thereof

Abstract

The application discloses a sample loading and collecting device for protein ultra-centrifugation process and an application method, relates to the experimental equipment field, and comprises a support, a power assembly, a guide assembly and a sample collecting device. The top of the support is provided with a fixing disc. The power assembly is fixedly installed on the fixing disc. An inclined pressing block is arranged at the power assembly. The side surface of the inclined pressing block is slidably connected to the sample collecting device. The sample collecting device is slidably connected in the guide assembly. The guide assembly is fixedly connected to the support. The bottom of the sample collecting device is provided with a sample collecting rubber plug. When the sample collecting rubber plug is driven by the power assembly to move downward, the tube cover and the centrifuge tube in the angular rotor can be taken out. A balance assembly is arranged on the power assembly. A balance frame corresponding to the centrifugal holes of the angular rotor in number is arranged in the balance assembly. The balance frame is used for helping to judge the sample balance during sample loading.

Description

Technical Field

[0001] This invention relates to the field of experimental equipment, specifically to a sample loading and receiving device and application method for protein ionization processes. Background Technology

[0002] Ultracentrifugation is a commonly used method for protein separation and purification. The usual method involves placing the protein in a sealed centrifuge tube, then placing the tube in a centrifuge. The high-speed rotation of the centrifuge achieves protein separation and enrichment.

[0003] In the process of loading centrifuge tubes into the centrifuge rotor, the loading and collection of samples after centrifugation are all done manually by laboratory personnel. This process is very tedious when there are many holes in the rotor. After loading the centrifuge tubes, the tube caps need to be installed one by one. However, the tube caps are small and usually cylindrical, making them very difficult to remove. Some tube caps are machined with removal threads, which are pulled out by screwing in bolts from the top, which is very tedious. This impacts the time and efficiency of centrifugation experiments, especially when the number of protein samples to be separated is less than the number of wells in the centrifuge. To ensure the rotation balance of the angular rotor, the samples need to be placed symmetrically into the wells. If there is no symmetrical setup during manual operation, for example, if the angular rotor has 12 wells and the samples include two types of proteins, one with two tubes and the other with three tubes, then the two-tube samples need to be placed symmetrically. After placing the two-tube samples, the three-tube samples of the other type must also be balanced in the centrifuge. If the five-tube samples are not placed symmetrically into the 12 wells of the angular rotor, the centrifuge will be under unbalanced forces, and the shaft torque will be different. When rotating at overspeed, the centrifuge is easily damaged, which not only affects the centrifugation results but also easily causes personnel injury. Moreover, different types of samples are easily confused when being taken out, leading to mixed loading and storage. Summary of the Invention

[0004] The purpose of this invention is to provide a sample loading and receiving device and application method for protein ultrafiltration processes, so as to solve the above-mentioned defects caused by the prior art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a sample loading and unloading device, comprising a support, a power component, a guide component, and a unloading device. The support has a fixed plate at its top, the power component is fixedly mounted on the fixed plate, and a slanted pressure block is provided at the location of the power component. The side of the slanted pressure block is slidably connected to the unloading device, and the unloading device is slidably connected within the guide component. The guide component is fixedly connected to the support, and the unloading device has a unloading rubber stopper at its bottom. When the unloading rubber stopper is driven downwards by the power component, it can remove the tube cap and centrifuge tube inside the angular rotor. The power component has a balancing component, which includes balancing frames in a number corresponding to the number of centrifuge holes of the angular rotor. The balancing frames are used to help determine the sample balance during loading.

[0006] Preferably, the power assembly includes a fixed head and a cylinder. The fixed head is fixedly mounted on a fixed plate, and the cylinder is fixedly mounted inside the fixed head. The output shaft of the cylinder is connected to the inclined pressure block.

[0007] Preferably, the guiding assembly includes a chuck and guide blocks. The chuck is fixedly connected to the inner wall of the bracket by a connecting rod. The number of guide blocks corresponds to the centrifugal holes of the angular rotor and is fixedly installed inside the chuck.

[0008] Preferably, the sample collection device includes a sealing tube, a push rod, a receiving cover, a connecting handle, and a sliding block. The top end of the sealing tube is fixedly connected to the connecting handle, and the lower end of the sealing tube is fixedly connected to the sample collection stopper. The upper end of the push rod is fixedly connected to the connecting handle, and a sealing element is provided below the push rod. The sealing element slides and seals within the sample collection stopper. The upper end of the receiving cover is fixedly connected to the lower end of the guide block, and the upper end of the connecting handle is connected to the sliding block.

[0009] Preferably, the inclined pressure block is a frustum-shaped block with a larger upper end and a smaller lower end. The side of the inclined pressure block is provided with a guide rail. The sliding block and the inclined pressure block are slidably connected through the guide rail. The lower end of the inclined pressure block is provided with a guide post. The bottom of the guide post can be inserted into the positioning hole at the center of the angular rotor.

[0010] Preferably, the sample receiving stopper is cylindrical, the bottom of the sample receiving stopper is provided with a guide slope, the inside of the sample receiving stopper is provided with a flared hole, the upper section of the flared hole is straight and the lower section is flared, the side of the flared hole is provided with a self-sealing hole, the self-sealing hole is provided with a fixing cover, the fixing cover is provided with a sealing membrane, and the sealing membrane includes two symmetrical parts that are closed at the front end.

[0011] Preferably, the balancing assembly includes a base, a rotating ring, a wear-resistant pad, and a pressure sensor. The base is fixedly mounted on the inclined pressure block, the rotating ring is rotatably mounted on the inclined pressure block and located above the base, the wear-resistant pad is fixedly mounted on the base and its upper end rests on the rotating ring, the pressure sensor is mounted on the rotating ring and its position corresponds to the balancing frame, and the lower part of the pressure sensor contacts the base.

[0012] Preferably, the tube cap is provided with a slot, and the opening of the slot is provided with a retaining ring.

[0013] An application method, characterized by comprising the following steps:

[0014] S1. Before loading the sample, a balance check is performed, including the following steps:

[0015] a. Clamp all centrifuge tube samples into the balancing rack based on experience;

[0016] b. Turn on the pressure sensor and use the digital feedback from the pressure sensor to determine whether the position of the centrifuge tube on the balancing assembly can keep the balancing assembly balanced.

[0017] c. When unbalanced, move the centrifuge tubes until they are balanced, and then copy the positions of the balanced centrifuge tubes one by one into the holes of the angular rotor.

[0018] S2: Insert the tube cap and perform ultracentrifugation;

[0019] S3: Collect the sample after centrifugation;

[0020] h. By pressing down the inclined block with the cylinder, the sample collection plug is pushed down into the slot;

[0021] i. Control the cylinder to reset, and remove the sample collection stopper and tube cap together from the centrifuge hole;

[0022] j. During the upward movement of the sample collection stopper, the tube cap will touch the collection cover and be pushed off the sample collection stopper by the collection cover as the sample collection stopper continues to move upward.

[0023] k. Control the cylinder to move down again, so that the sample collection stopper extends into the centrifuge hole and presses against the cap of the centrifuge tube. As the pressure continues to be applied, the lower end of the sample collection stopper deforms, and at the same time the push rod moves down to release the gas in the sample collection stopper by pushing open the sealing membrane.

[0024] 1. After the gas is discharged, the sample collection stopper is moved upward by the cylinder. The sample collection stopper expands and forms a negative pressure, which adsorbs the centrifuge tube and removes the centrifuge tube from the centrifuge, thus completing the sample collection.

[0025] Compared with the prior art, the beneficial effects of the present invention are:

[0026] 1. The present invention provides a balancing component for pre-evaluating whether different types and quantities of centrifuge tubes can maintain balance, which can obtain the balance result before loading the sample, and prevent centrifuge rotation imbalance caused by multiple types and quantities of centrifuge tubes not being able to maintain balance on the centrifuge, thus affecting the results;

[0027] 2. The tube caps with slots are designed to be collected from the centrifuge using a storage cover during sample collection, ensuring unified storage and preventing loss.

[0028] 3. The sample collection stopper allows multiple centrifuge tubes to be removed from the centrifuge at once, avoiding sample mixing and storage when different types of samples are removed one by one, which facilitates the summarization of experimental results and data statistics. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the structure of the present invention;

[0030] Figure 2 This is a top view of the present invention;

[0031] Figure 3 for Figure 2 Cross-sectional structural diagram of the inner BB section;

[0032] Figure 4 for Figure 3 Enlarged view of the structure at point C;

[0033] Figure 5 for Figure 3 Enlarged view of the structure at point D;

[0034] Figure 6 This is a cross-sectional structural diagram of the sample collection device;

[0035] Figure 7 for Figure 6 Enlarged view of the structure at point E;

[0036] Figure 8 This is a schematic diagram of the inclined pressure block structure;

[0037] Figure 9 This is a schematic diagram of the balancing component structure.

[0038] Wherein: 1-bracket; 11-fixed plate;

[0039] 2-Power assembly; 21-Fixed head; 22-Cylinder;

[0040] 3-Guide assembly; 31-Chuck; 32-Guide block; 33-Connecting rod;

[0041] 4-Sample receiving device; 41-Sample receiving rubber stopper; 411-Guide inclined surface; 412-Flare hole; 413-Self-sealing hole;

[0042] 414 - Fixing cover; 415 - Sealing membrane;

[0043] 42-Sealing tube; 43-Push rod; 44-Receiving cover; 45-Connecting handle; 46-Sliding block;

[0044] 5- Inclined pressure block; 51- Guide rail; 52- Guide post;

[0045] 6-Balancing assembly; 61-Balancing frame; 62-Base; 63-Rotating ring; 64-Wear-resistant pad; 65-Pressure sensor;

[0046] 100-Angle rotor; 101-Pipe cap; 102-Center tube; 103-Positioning hole; 1011-Slot; 1012-Snap ring. Detailed Implementation

[0047] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0048] Please see Figures 1 to 9 This invention provides a technical solution comprising a support 1, a power component 2, a guide component 3, and a sample receiving device 4. The support 1 has a fixed plate 11 at its top, the power component 2 is fixedly mounted on the fixed plate 11, and a slanted pressure block 5 is provided at the location of the power component 2. The side of the slanted pressure block 5 is slidably connected to the sample receiving device 4, and the sample receiving device 4 is slidably connected inside the guide component 3. The guide component 3 is fixedly connected to the support 1, and the bottom of the sample receiving device 4 has a sample receiving rubber stopper 41. When the sample receiving rubber stopper 41 is moved downwards by the power component 2, it can remove the tube cap 101 and centrifuge tubes 102 inside the angle rotor 100. The power component 2 has a balancing component 6, which contains a number of balancing frames 61 corresponding to the number of centrifuge holes on the angle rotor. The balancing frames 61 are used to help determine the sample balance during loading. In this embodiment, an 8-hole angle rotor 100 is used as an example, and the angle rotor 100 can hold a maximum of 8 centrifuge tubes 102.

[0049] In this embodiment, the power assembly 2 includes a fixed head 21 and a cylinder 22. The fixed head 21 is fixedly mounted on the fixed plate 11, and the cylinder 22 is fixedly mounted inside the fixed head 21. The output shaft of the cylinder 22 is connected to the inclined pressure block 5, and the inclined pressure block 5 below can move up and down by the drive of the cylinder 22.

[0050] In this embodiment, the guide assembly 3 includes a chuck 31 and guide blocks 32. The chuck 31 is fixedly connected to the inner wall of the bracket 1 by a connecting rod 33. The number of guide blocks 32 corresponds to the centrifugal holes of the angular rotor and is fixedly installed in the chuck 31. The guide blocks 32 are fixed in different positions to provide guidance for the connecting handle 45 passing through the guide blocks 32.

[0051] In this embodiment, the sample collection device 4 includes a sealing tube 42, a push rod 43, a receiving cover 44, a connecting handle 45, and a sliding block 46. The top end of the sealing tube 42 is fixedly connected to the connecting handle 45, and the lower end of the sealing tube 42 is fixedly connected to the sample collection stopper 41. The upper end of the push rod 43 is fixedly connected to the connecting handle 45, and a sealing element is provided below the push rod 43. The sealing element slides and seals within the sample collection stopper 41. The upper end of the receiving cover 44 is fixedly connected below the guide block 32, and the upper end of the connecting handle 45... Connected to the sliding block 46, the sample collection plug 41 is cylindrical, with a guide slope 411 at the bottom. The inside of the sample collection plug 41 has a flared hole 412, the upper section of which is straight and the lower section is flared. A self-sealing hole 413 is provided on the side of the flared hole 412, and a fixing cover 414 is provided inside the self-sealing hole 413. A sealing membrane 415 is provided inside the fixing cover 414, and the sealing membrane 415 comprises two symmetrical parts with closed front ends. The sample collection process of the sample collection device 4 includes two steps:

[0052] 1. When the inclined pressure block 5 is pressed down, the sliding block 46 presses down the sealing tube 42 and the push rod 43 and moves them together. At this time, the two will move the sample collection plug 41 and insert it into the slot 1011 of the tube cap 101. Since the diameter of the retaining ring 1012 on the slot 1011 is smaller than that of the sample collection plug 41, the sample collection plug 41 will hold the tube cap 101. When the cylinder 22 moves the sample collection plug 41 upward, the tube cap 101 will be completely removed from the angle rotor 100. Then, as it continues to move upward, the tube cap 101 enters the storage cover 44. The internal curved surface of the storage cover 44 pushes the tube cap 101 down from the sample collection plug 41 for unified storage.

[0053] 2. After collecting the centrifuge tubes 102 and completing the collection of the tube caps 101, control the sample collection stopper 41 to move down again. The bottom surface of the sample collection stopper 41 abuts against the cap of the centrifuge tubes 102. As it continues to be pressed down, the sample collection stopper 41, being made of rubber, will deform, and the volume of the internal horn hole 412 will decrease. At the same time, as the sample collection stopper 41 is compressed, the push rod 43 continues to press down, and the air pressure inside the horn hole 412 increases, causing the gas to burst through the sealing membrane 415 and exit the horn hole 412. After reaching a certain level, control the sample collection stopper 41 to rise. In this way, the sample collection stopper 41 will use air pressure to hold the centrifuge tubes 102, collecting all the centrifuge tubes from the centrifuge rotor at once.

[0054] In this embodiment, the inclined pressure block 5 is a frustum-shaped block with a larger upper end and a smaller lower end. The side of the inclined pressure block 5 is provided with a guide rail 51. The sliding block 46 is slidably connected to the inclined pressure block 5 through the guide rail 51. The lower end of the inclined pressure block 5 is provided with a guide post 52. The bottom of the guide post 52 can be inserted into the positioning hole 103 at the center of the angle rotor 100. The guide post 52 can maintain the vertical downward pressure state when the inclined pressure block 5 is pressed down and subjected to force, preventing deviation.

[0055] In this embodiment, the balancing assembly 6 includes a base 62, a rotating ring 63, a wear-resistant pad 64, and a pressure sensor 65. The base 62 is fixedly mounted on the inclined pressure block 5. The rotating ring 63 is rotatably mounted on the inclined pressure block 5 and located above the base 62. The wear-resistant pad 64 is fixedly mounted on the base 62 and its upper end rests on the rotating ring 63. The pressure sensor 65 is mounted on the rotating ring 63 and its position corresponds to that of the balancing frame 61. The lower part of the pressure sensor 65 contacts the base 62. The centrifuge tubes 102 of the samples that need to be balanced are placed in the balancing frame 61. For example, if two types of protein samples, one with two tubes and the other with three tubes, need to be placed in the balancing frame 61, then the rotating ring 63 is slowly rotated manually. The data from the pressure sensor 65 is read during the movement to determine whether the five sample tubes are balanced during the rotation.

[0056] In this embodiment, the tube cap 101 is provided with a slot 1011, and the opening of the slot 1011 is provided with a retaining ring 1012. The slot 1011 is used together with the sample collection plug 41.

[0057] Working principle: First, place the centrifuge tubes 102 that need to be balanced into the balancing rack 61. Then, manually and slowly rotate the rotating ring 63, reading the data from the pressure sensor 65 during the movement to determine whether the five sample tubes are balanced. Once balanced, place all the samples into the centrifuge one by one according to their balanced positions and start centrifugation. After centrifugation, collect the tube cap 101. When the inclined pressure block 5 presses down, the sliding block 46 presses down the sealing tube 42 and the push rod 43, causing them to move together. At this time, the two will move the sample collection plug 41 and insert it into the slot 1011 of the tube cap 101. Since the diameter of the retaining ring 1012 on the slot 1011 is smaller than that of the sample collection plug 41, the sample collection plug 41 will hold the tube cap 101 in place. When the cylinder 22 moves the sample collection plug 41 upward, the tube cap 101 will be completely removed from the angle rotor 100. As the centrifuge tubes continue to move upward, the tube cap 101 enters the storage cover 44. Through the internal curved surface of the storage cover 44, the tube cap 101 is pushed down from the sample collection stopper 41 for unified storage. Then, the centrifuge tubes 102 are collected. After the tube cap 101 is collected, the sample collection stopper 41 is controlled to move downward again. The bottom surface of the sample collection stopper 41 abuts against the cap of the centrifuge tubes 102. As it continues to be pressed down, the sample collection stopper 41, being made of rubber, will deform, and the volume of the internal horn hole 412 will decrease. At the same time, as the sample collection stopper 41 is compressed, the push rod 43 continues to press down, and the air pressure inside the horn hole 412 increases, causing the gas to burst through the sealing membrane 415 and exit the horn hole 412. After reaching a certain level, the sample collection stopper 41 is controlled to rise. In this way, the sample collection stopper 41 will use air pressure to hold the centrifuge tubes 102, collecting all the centrifuge tubes from the centrifuge tubes at once, and then summarizing and collecting them.

[0058] An application method, characterized by comprising the following steps:

[0059] S1. Before loading the sample, a balance check is performed, including the following steps:

[0060] a. Clamp all the centrifuge tubes 102 samples into the balance rack 61 based on experience;

[0061] b. Turn on the pressure sensor 65 and use the digital feedback of the pressure sensor 65 to determine whether the position of the centrifuge tube 102 on the balancing assembly 6 can keep the balancing assembly balanced.

[0062] c. When unbalanced, move the position of centrifuge tube 102 until it is balanced, and then copy the position of the balanced centrifuge tube 102 into the hole of the angle rotor 100.

[0063] S2: Place the tube cap 101 in the tube and perform ultracentrifugation;

[0064] S3: Collect the sample after centrifugation;

[0065] h. The cylinder 22 presses down the inclined block 5, pushing the sample collection plug 41 down into the slot 1011;

[0066] i. Control cylinder 22 to reset, and remove sample collection plug 41 and tube cap 101 together from the centrifuge hole;

[0067] j. During the upward movement of the sample collection stopper 41, the tube cap 101 will touch the storage cover 44 and be pushed off the sample collection stopper 41 by the storage cover 44 as the sample collection stopper 41 continues to move upward.

[0068] k. Control the cylinder 22 to move down again, so that the sample collection plug 41 extends into the centrifuge hole and presses against the cap of the centrifuge tube 102. As the pressure continues to be applied, the lower end of the sample collection plug 41 deforms, and at the same time the push rod 43 moves down to discharge the gas in the sample collection plug 41 by pushing open the sealing membrane 415.

[0069] 1. After the gas is discharged, the sample collection stopper 41 is moved upward by the cylinder 22. The sample collection stopper 41 expands and forms a negative pressure, which adsorbs the centrifuge tube 102 and removes the centrifuge tube 102 from the centrifuge, thus completing the sample collection.

[0070] Based on the above, the present invention provides a balancing component for pre-evaluating whether different types and quantities of centrifuge tubes can maintain balance. This allows for obtaining balance results before sample loading, preventing centrifuge rotation imbalance caused by multiple types and quantities of centrifuge tubes not maintaining balance on the centrifuge, which would affect the results.

[0071] As is known from common technical knowledge, this invention can be implemented through other embodiments that do not depart from its spirit or essential characteristics. Therefore, the disclosed embodiments described above are merely illustrative and not exhaustive. All modifications within the scope of this invention or its equivalents are included in this invention.

Claims

1. A sample loading and receiving device, characterized in that: The device includes a support (1), a power assembly (2), a guide assembly (3), and a sample receiving device (4). The top of the support (1) is provided with a fixed plate (11). The power assembly (2) is fixedly installed on the fixed plate (11). The bottom of the power assembly (2) is provided with a slanted pressure block (5). The side of the slanted pressure block (5) is slidably connected to the sample receiving device (4). The sample receiving device (4) is slidably connected inside the guide assembly (3). The guide assembly (3) is fixedly connected to the support (1). The bottom of the sample receiving device (4) is provided with a sample receiving rubber plug (41). When the sample receiving rubber plug (41) is driven downward by the power assembly (2), it can remove the tube cap (101) and centrifuge tube (102) inside the angle rotor (100). The power assembly (2) is provided with a balancing assembly (6). The balancing assembly (6) is provided with a number of balancing frames (61) corresponding to the number of centrifuge holes of the angle rotor. The balancing frames (61) are used to help determine the sample balance when loading the sample. The sample collection device (4) includes a sealing tube (42), a push rod (43), a receiving cover (44), a connecting handle (45), and a sliding block (46). The top end of the sealing tube (42) is fixedly connected to the connecting handle (45), and the lower end of the sealing tube (42) is fixedly connected to the sample collection plug (41). The upper end of the push rod (43) is fixedly connected to the connecting handle (45). A sealing element is provided below the push rod (43). The sealing element slides and seals inside the sample collection plug (41). The upper end of the receiving cover (44) is fixedly connected to the lower end of the guide block (32), and the upper end of the connecting handle (45) is connected to the sliding block (46). The sample collection stopper (41) is cylindrical. The bottom of the sample collection stopper (41) is provided with a guide slope (411). The inside of the sample collection stopper (41) is provided with a horn hole (412). The upper part of the horn hole (412) is straight and the lower part is horn-shaped. The side of the horn hole (412) is provided with a self-sealing hole (413). The self-sealing hole (413) is provided with a fixing cover (414). The fixing cover (414) is provided with a sealing membrane (415). The sealing membrane (415) includes two symmetrical parts with the front end closed. The balancing assembly (6) includes a base (62), a rotating ring (63), a wear-resistant pad (64), and a pressure sensor (65). The base (62) is fixedly mounted on the inclined pressure block (5). The rotating ring (63) is rotatably mounted on the inclined pressure block (5) and located above the base (62). The wear-resistant pad (64) is fixedly mounted on the base (62) and its upper end rests on the rotating ring (63). The pressure sensor (65) is mounted on the rotating ring (63) and its position corresponds to that of the balancing frame (61). The lower part of the pressure sensor (65) contacts the base (62).

2. The sample loading and receiving device according to claim 1, characterized in that: The power assembly (2) includes a fixed head (21) and a cylinder (22). The fixed head (21) is fixedly mounted on the fixed plate (11), and the cylinder (22) is fixedly mounted inside the fixed head (21). The output shaft of the cylinder (22) is connected to the inclined pressure block (5).

3. The sample loading and receiving device according to claim 2, characterized in that: The guide assembly (3) includes a chuck (31) and guide blocks (32). The chuck (31) is fixedly connected to the inner wall of the bracket (1) by a connecting rod (33). The number of guide blocks (32) corresponds to the centrifugal holes of the angular rotor and is fixedly installed in the chuck (31).

4. The sample loading and receiving device according to claim 3, characterized in that: The inclined pressure block (5) is a frustum shape with a large upper end and a small lower end. The side of the inclined pressure block (5) is provided with a guide rail (51). The sliding block (46) and the inclined pressure block (5) are slidably connected through the guide rail (51). The lower end of the inclined pressure block (5) is provided with a guide post (52). The bottom of the guide post (52) can be inserted into the positioning hole (103) at the center of the angle rotor (100).

5. A sample loading and receiving device according to claim 4, characterized in that: The tube cap (101) is provided with a slot (1011), and the opening of the slot (1011) is provided with a retaining ring (1012).

6. A method for loading and unloading samples using the sample loading and unloading device according to claim 5, characterized in that: Includes the following steps: S1. Before loading the sample, a balance check is performed, including the following steps: a. Clamp all the centrifuge tubes (102) into the balance rack (61) based on experience; b. Turn on the pressure sensor (65) and use the digital feedback of the pressure sensor (65) to determine whether the position of the centrifuge tube (102) on the balancing assembly (6) can keep the balancing assembly balanced; c. When unbalanced, move the position of the centrifuge tube (102) until it is balanced, and then copy the position of the centrifuge tube (102) that can be balanced to the holes of the angle rotor (100); S2: Insert the tube cap (101) and perform ultracentrifugation; S3: Collect the sample after centrifugation; h. By pressing down the inclined block (5) with the cylinder (22), the sample collection plug (41) is pushed down into the slot (1011); i. Control the cylinder (22) to reset, and remove the sample collection plug (41) and the tube cap (101) together from the centrifuge hole; j. During the upward movement of the sample receiving rubber stopper (41), the tube cap (101) will touch the receiving cover (44) and be pushed off the sample receiving rubber stopper (41) by the receiving cover (44) as the sample receiving rubber stopper (41) continues to move upward; k. Control the cylinder (22) to move down again, so that the sample collection plug (41) extends into the centrifuge hole and presses against the cap of the centrifuge tube (102). As the pressure continues to be applied, the lower end of the sample collection plug (41) deforms, and at the same time the push rod (43) moves down to discharge the gas in the sample collection plug (41) by pushing open the sealing membrane (415). l. After the gas is discharged, the sample collection stopper (41) is moved upward by the cylinder (22). The sample collection stopper (41) expands and forms a negative pressure, adsorbing the centrifuge tube (102). The centrifuge tube (102) is then removed from the centrifuge, and the sample collection is completed.

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