Isothermal amplification device

Abstract

The present invention relates to the technical field of isothermal amplification, and specifically to an isothermal amplification device. The isothermal amplification device comprises an outer shell, a conveying groove is provided in the outer shell, a plurality of support rods are arranged on the inner peripheral wall of the conveying groove, two U-shaped blocks are fixedly connected at both ends of the plurality of support rods, balls are rotatably connected to the inner peripheral wall of the U-shaped block, the support rods contact with the inner peripheral wall of the conveying groove through the balls, the outer peripheral walls of the plurality of support rods are fixedly connected to base blocks, the outer peripheral walls of the plurality of support rods are sleeved and connected with compression springs, the outer peripheral walls of the plurality of support rods are slidably connected with collars, and the two ends of the compression springs are respectively fixedly connected to the base blocks and the collars. The beneficial effects of the present invention are as follows: the isothermal amplification device combines the method of applying pressure to the space where DNA is located with the nuclear amplification technology to amplify DNA, so that the amplification work of the device has a continuous effect, and the pressure of the device is in a controllable state, and the user can still make the work of taking and placing DNA more convenient.

Description

Technical Field

[0001] The invention relates to the technical field of isothermal amplification, in particular to an isothermal amplification device. Background Art

[0002] Isothermal amplification is a new type of nucleic acid amplification technology based on constant temperature amplification. Its principle mainly includes the use of special enzymes and primer designs to enable DNA amplification under constant temperature conditions. These special enzymes can be activated at lower temperatures, thereby realizing amplification reactions under constant temperature conditions. At the same time, the design of primers is also crucial. They can recognize and bind to the target DNA sequence and amplify under the action of the enzyme. In addition, isothermal amplification also utilizes the principle of cyclic amplification, that is, the products produced during the reaction can be used as new templates to participate in the next round of amplification reactions, thereby achieving rapid and efficient DNA amplification; the advantages of isothermal amplification technology include rapidity, efficiency, simplicity and ease of operation, high sensitivity, and strong specificity. This technology has a wide range of applications in nucleic acid detection, gene cloning, sequencing, etc., and is suitable for some scenarios that require rapid detection; currently, isothermal amplification has been successfully applied in the field of diagnosis and occupies an important position in commercial molecular diagnostic platforms;

[0003] However, the isothermal amplification device can only perform a single amplification at a time during use, which affects the user's work in nucleic acid testing, gene cloning, sequencing, etc.

[0004] However, it is difficult for existing isothermal amplification devices to continuously amplify a variety of different DNAs, which may affect the user's work and waste the user's time, thereby making the use effect of the device less than ideal. Summary of the invention

[0005] The present invention aims at the technical problems existing in the prior art and provides an isothermal amplification device to solve the above-mentioned problem that the existing isothermal amplification device is difficult to continuously amplify a variety of different DNAs.

[0006] The technical solution of the present invention for solving the above technical problems is as follows: an isothermal amplification device comprises an outer shell, a conveying groove is opened in the outer shell, a plurality of support rods are arranged on the inner circumferential wall of the conveying groove, two U-shaped blocks are fixedly connected at both ends of the plurality of support rods, balls are rotatably connected to the inner circumferential wall of the U-shaped block, the support rods contact with the inner circumferential wall of the conveying groove through the balls, the outer circumferential walls of the plurality of support rods are fixedly connected to the base block, the outer circumferential walls of the plurality of support rods are sleevedly connected with compression springs, the outer circumferential walls of the plurality of support rods are slidably connected with collars, the two ends of the compression springs are respectively fixedly connected to the base block and the collar, the outer circumferential wall of the collar is fixedly connected with a support block, every two support rods are arranged into a group through a support block, the support block extends to the outside of the outer shell through the opening of the conveying groove, two triangular blocks are fixedly connected to the top of the support block, circular grooves are opened at opposite positions of the two triangular blocks, and a roller is rotatably connected to the inner circumferential wall of the circular groove.

[0007] The beneficial effects of the present invention are:

[0008] This isothermal amplification device has better effect. The present invention is improved on the existing basis. When the support block is located at the U-shaped guide rod, the distance between the reaction vessel and the detection component is enlarged, so that the device cannot amplify the DNA at this time, and the user can take the reaction vessel at this position to replace different DNA supply devices for amplification, thereby making the amplification work of the device sustainable and convenient for the user to replace different DNAs in the reaction vessel to supply the device for amplification.

[0009] Based on the above technical solution, the present invention can also be improved as follows.

[0010] Furthermore, the inner wall of the conveying trough is provided with multiple hydraulic rods, the ends of the hydraulic rods are fixedly connected to guide rails, and buffer grooves are provided at both ends of the guide rails. The inner walls of the two buffer grooves are slidably connected to limit slide rods, and the ends of the two limit slide rods are fixedly connected to screw sleeves, and the inner walls of the two screw sleeves are threadedly connected to rotating screws, and the ends of the two limit slide rods are provided with connecting grooves, and the inner walls of the connecting grooves are penetrated and connected with U-shaped guide rods, and the guide rails and the U-shaped guide rods are in conflict with the roller.

[0011] The beneficial effect of adopting the above further scheme is that the height of the guide rail can be adjusted as needed, and after the adjustment, the user can further change the height of the U-shaped guide rod by rotating the rotating screw rod, and the distance between the bottom of the U-shaped guide rod and the height of the guide rail can be changed synchronously after this change, and then the distance between the two can be adjusted by rotating the rotating screw rod by external force, so that the position here can still facilitate the user to replace the DNA after the height of the guide rail is adjusted.

[0012] Furthermore, a pad is fixedly connected to one side of the support block, an anti-slip wall is provided on the top of the pad, a reaction vessel is arranged on the top of the anti-slip wall, a sealing ring is fixedly connected to the inner circumferential wall of the reaction vessel, a side opening is provided on one side of the reaction vessel, an elastic sealing block is provided on the inner circumferential wall of the side opening, a sliding cover is slidably connected to the bottom of the elastic sealing block, a detection component is fixedly connected to the outer circumferential wall of the support rod, a piston is fixedly connected to the bottom of the detection component, the piston is located on the inner circumferential wall of the reaction vessel, a built-in groove is provided at the bottom of the detection component, and the inner diameter of the built-in groove is adapted to the thickness of the reaction vessel.

[0013] The beneficial effect of adopting the above further scheme is that, by setting a sealing ring, the sealing ring can always be wrapped around the outer peripheral wall of the piston during its movement, which can indirectly improve the sealing effect of the reaction vessel and prevent the DNA therein from being affected by air. At the same time, in the process of the piston moving downward based on the reaction vessel, the reaction vessel is sealed by the sliding cover before the piston enters the reaction vessel. After the piston contacts the sliding cover, since the top of the sliding cover is inclined, the downward pressure applied by the piston to the sliding cover can be converted into side pressure, and then the sliding cover is moved toward the side opening direction, thereby exposing the space at the bottom of the sliding cover, making it easier for the piston to apply pressure to the DNA at this position. On this basis, the nucleic acid amplification technology is combined to perform corresponding amplification work on the DNA, and the auxiliary device amplifies the DNA. Through the action of the sliding cover, the DNA can be prevented from being affected by air, thereby ensuring the accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0015] Figure 2 yes Figure 1 The enlarged structural diagram at A in the middle;

[0016] Figure 3 yes Figure 1 The enlarged structural diagram at B in the middle;

[0017] Figure 4 It is a top cross-sectional schematic diagram of the present invention;

[0018] Figure 5 It is a partial side cross-sectional structural schematic diagram of the present invention;

[0019] Figure 6 It is a front cross-sectional structural schematic diagram of the reaction vessel in the present invention;

[0020] Figure 7 It is a schematic diagram of the top cross-sectional structure of the support block in the present invention.

[0021] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0022] 1. Shell; 2. Conveying trough; 3. Hydraulic rod; 301. Guide rail; 302. Buffer trough; 303. Limiting slide rod; 304. Screw sleeve; 305. Rotating screw; 306. Connecting groove; 307. U-shaped guide rod; 4. Support rod; 401. U-shaped block; 402. Ball; 403. Base block; 404. Compression spring; 405. Ring; 406. Support block; 407. Triangular block; 408. Circular groove; 409. Roller; 5. Cushion block; 501. Anti-slip wall; 502. Reactor vessel; 503. Sealing ring; 504. Side opening; 505. Sliding cover; 506. Elastic sealing block; 6. Detection component; 601. Built-in groove; 602. Piston. DETAILED DESCRIPTION

[0023] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0024] Isothermal amplification is a new type of nucleic acid amplification technology based on constant temperature amplification. Its principle mainly includes the use of special enzymes and primers to enable DNA amplification under constant temperature conditions. These special enzymes can be activated at a lower temperature, thereby realizing an amplification reaction under constant temperature conditions. At the same time, the design of primers is also crucial. They can recognize and bind to the target DNA sequence and amplify under the action of the enzyme. In addition, isothermal amplification also utilizes the principle of cyclic amplification, that is, the product produced during the reaction can be used as a new template to participate in the next round of amplification reaction, thereby achieving rapid and efficient DNA amplification; the advantages of isothermal amplification technology include rapidity, efficiency, simplicity and ease of operation, high sensitivity, and strong specificity. This technology has a wide range of applications in nucleic acid detection, gene cloning, sequencing, etc., and is suitable for some scenarios that require rapid detection; at present, isothermal amplification has been successfully applied to the field of diagnosis and occupies an important position in commercial molecular diagnostic platforms. In this regard, the inventors have proposed an isothermal amplification device to solve the above problems.

[0025] The present invention provides the following preferred embodiments

[0026] like Figure 1-7As shown, an isothermal amplification device includes a shell 1, a conveying groove 2 is opened in the shell 1, and the inner peripheral wall of the conveying groove 2 is provided with a plurality of support rods 4, both ends of the plurality of support rods 4 are fixedly connected to two U-shaped blocks 401, the inner peripheral wall of the U-shaped block 401 is rotatably connected to a ball 402, the support rod 4 contacts the inner peripheral wall of the conveying groove 2 through the ball 402, the outer peripheral walls of the plurality of support rods 4 are fixedly connected to a base block 403, the outer peripheral walls of the plurality of support rods 4 are sleeved and connected to a compression spring 404, the outer peripheral walls of the plurality of support rods 4 are slidably connected to a collar 405, the two ends of the compression spring 404 are fixedly connected to the base block 403 and the collar 405 respectively, the outer peripheral wall of the collar 405 is fixedly connected to a support block 406, and every two support rods 4 are arranged to form a The support block 406 extends out of the housing 1 through the opening of the conveying trough 2. Two triangular blocks 407 are fixedly connected to the top of the support block 406. Circular grooves 408 are provided at opposite positions of the two triangular blocks 407. The inner circumferential wall of the circular groove 408 is rotatably connected with a roller 409. The support rod 4 is arranged in the conveying trough 2, and the U-shaped block 401 fixed at the end of the support rod 4 limits the ball 402, so that the ball 402 can roll freely on the inner circumferential wall of the U-shaped block 401, and the ball 402 is allowed to conflict with the inner circumferential wall of the conveying trough 2, and then the support rod 4 can slide in the conveying trough 2 under the action of the ball 402. Since every two support rods 4 can form a group after being connected through the support block 406, a type of support rod 4 is formed. The structure is similar to the H-shaped state, so that the tightness between the two support rods 4 in the same group can be increased, so that the four balls 402 at the ends of the two support rods 4 are in conflict with the inner wall of the conveying trough 2, which can further prevent the bracket 4 from tilting. Then, based on this, the base block 403, the compression spring 404 and the ring 405 are arranged on the outer wall of the support rod 4, and the base block 403 and the support rod 4 are fixedly connected, and then the compression spring 404 can use the base block 403 as the force point, so that the ring 405 can have the elasticity of upward displacement under the action of the compression spring 404, thereby driving the support block 406 and other structures fixed thereto to move upward, and then the roller 409 arranged on the top of the support block 406 conflicts with the guide rail 301 and the U-shaped guide rod 307, and then The support block 406 can be moved according to the height and trajectory of the guide rail 301 and the U-shaped guide rod 307, and because the roller 409 is engaged with the circular groove 408 between the two triangular blocks 407, the roller 409 can rotate in the circular groove 408, and then the roller 409, the triangular block 407 and the guide rail 301 can roll, thereby indirectly reducing the friction between the triangular block 407 and other structures and the guide rail 301, so that the reaction vessel 502 can move better based on the guide rail 301, and the movement effect tends to be stable, and then multiple groups of support rods 4 and support blocks 406 are arranged based on this, so that the device can contain multiple groups of components that can perform isothermal amplification of DNA.Thus, the device can simultaneously amplify multiple different DNAs. Since the height of the detection component 6 connected to the top of the support rod 4 remains unchanged during the movement, the components connected to the cushion block 5 connected to the bottom through the support block 406 can be shifted up and down based on the guide rail 301 and the U-shaped guide rod 307, so that the distance between the cushion block 5 and the detection component 6 changes. Based on this, the device amplifies the DNA in the reaction vessel 502. Then, when the support block 406 is located at the U-shaped guide rod 307, the distance between the reaction vessel 502 and the detection component 6 is expanded, so that the device cannot amplify the DNA at this time, and it is convenient for the user to take the reaction vessel at this position to replace different DNAs for the device to perform amplification. Therefore, the amplification work of the device is sustainable, and it is convenient for the user to replace different DNAs in the reaction vessel 502 to supply the device for amplification.

[0027] In this embodiment, Figure 1 , Figure 3 and Figure 4As shown, in order to further improve the controllable effect of the amplification device on DNA amplification, the inner circumferential wall of the conveying groove 2 is provided with multiple hydraulic rods 3, the ends of the hydraulic rods 3 are fixedly connected to the guide rails 301, and the two ends of the guide rails 301 are provided with buffer grooves 302. The inner circumferential walls of the two buffer grooves 302 are slidably connected to the limiting sliding rods 303, and the ends of the two limiting sliding rods 303 are fixedly connected to the screw sleeves 304. The inner circumferential walls of the two screw sleeves 304 are threadedly connected to the rotating screws 305, and the ends of the two limiting sliding rods 303 are provided with connecting grooves 306. The inner wall of the connecting groove 306 is penetrated and connected with a U-shaped guide rod 307. The guide rail 301 and the U-shaped guide rod 307 both conflict with the roller 409. The hydraulic rod 3 is fixed to the inner wall of the conveying groove 2, and the guide rail 301 is fixed at the end of the hydraulic rod 3. Then, according to the extension and contraction of the hydraulic rod 3, the height of the guide rail 301 is controlled, and the distance between the reaction vessel 502 and the detection component 6 is further controlled through the conflict effect between the guide rail 301 and the roller 409. On this basis, the buffer grooves 302 opened at both ends of the guide rail 301 and the roller 409 that can slide in the buffer groove 302 are used to control the distance between the reaction vessel 502 and the detection component 6. The limiting slide bar 303 is limited in the buffer groove 302 and cannot rotate. Then, the two ends of the guide rail 301 are connected by the screw sleeve 304 and the rotating screw rod 305. After the rotating screw rod 305 is rotated by an external force, the two screw sleeves 304 connected thereto by a threaded relationship can be offset under this action, thereby changing the distance between the two screw sleeves 304 and causing the limiting slide bar 303 to slide accordingly in the buffer groove 302. After this distance changes, the two limiting slide bars 303 are opened. After the U-shaped guide rod 307 is connected to the guide rail 301, the two connecting grooves 306 in the position slide bar 303 can make the two ends of the U-shaped guide rod 307 pass through the connecting grooves 306 and move based on the change in the spacing between the two connecting grooves 306, that is, the bottom of the U-shaped guide rod 307 can change synchronously with the height of the guide rail 301 after this change, and then the spacing between the two can be adjusted by rotating the rotating screw rod 305 by external force, so that the position here can still be convenient for users to replace DNA after the height of the guide rail 301 is adjusted.

[0028] In this embodiment, Figure 5 and Figure 6As shown, in order to further enhance the self-assisting effect of the amplification device during amplification, a cushion block 5 is fixedly connected to one side of the support block 406, a non-slip wall 501 is provided on the top of the cushion block 5, a reaction vessel 502 is arranged on the top of the non-slip wall 501, a sealing ring 503 is fixedly connected to the inner peripheral wall of the reaction vessel 502, a side opening 504 is provided on one side of the reaction vessel 502, an elastic sealing block 506 is provided on the inner peripheral wall of the side opening 504, a sliding cover 505 is slidably connected to the bottom of the elastic sealing block 506, a detection component 6 is fixedly connected to the outer peripheral wall of the support rod 4, and the bottom of the detection component 6 A piston 602 is fixedly connected, and the piston 602 is located on the inner wall of the reaction vessel 502. A built-in groove 601 is opened at the bottom of the detection component 6. The inner diameter of the built-in groove 601 is adapted to the thickness of the reaction vessel 502. The cushion block 5 is connected to the support rod 4 through the support block 406, so that the height of the cushion block 5 can be indirectly controlled according to the guide rail 301 and the U-shaped guide rod 307. Since the detection component 6 is directly fixed to the support rod 4, its height cannot be changed. Based on this, the reaction vessel 502 is placed on the top of the anti-slip wall 501 to avoid the reaction vessel 5 02 slides, and then the piston 602 fixed at the bottom of the detection component 6 is located at the top of the reaction vessel 502 and deviates downward based on the reaction vessel 502. In this process, by providing a sealing ring 503, the piston 602 can always be wrapped around its outer peripheral wall during the movement of the piston 602, which can indirectly improve the sealing effect of the reaction vessel 502 and prevent the DNA therein from being affected by air. At the same time, when the piston 602 moves downward based on the reaction vessel 502, the sliding cover 505 seals the reaction vessel 502 before the piston 602 enters the reaction vessel 502, and After the plug 602 contacts the sliding cover 505, due to the inclined top of the sliding cover 505, the downward pressure applied by the piston 602 to the sliding cover 505 can be converted into lateral pressure, and then the sliding cover 505 is moved toward the side opening 504, thereby exposing the bottom space of the sliding cover 505, making it easier for the piston 602 to apply pressure to the DNA at this position, and on this basis, combined with the nucleic acid amplification technology, the DNA is amplified accordingly, and the auxiliary device amplifies the DNA. Through the action of the sliding cover 505, the DNA can be prevented from being affected by the air, thereby ensuring the accuracy of the test results.

[0029] The specific working process of the present invention is as follows:

[0030] (1) Adjustment

[0031] First, according to the pressure required by the device to amplify the working DNA, the height of the guide rail 301 is adjusted so that the work between the reaction vessel 502 and the piston 602 can be changed accordingly;

[0032] (2) Placement

[0033] Then, different DNAs are placed in a plurality of reaction vessels 502 , and the reaction vessels 502 are connected to the pads 5 in sequence, so that the reaction vessels 502 containing the DNA are located at the bottom of the piston 602 .

[0034] (3) Mobile

[0035] Then, during the placement process, as the number of reaction vessels 502 increases, the reaction vessels 502 located at the U-shaped guide rod 307 can move toward the guide rail 301, so that the device can amplify multiple DNAs at a time.

[0036] (4) Pressure

[0037] Finally, when the reaction vessel 502 is located at the guide rail 301, as the structure abutted by the roller 409 changes, the distance between the reaction vessel 502 and the piston 602 changes, so that the piston 602 can indirectly apply pressure to the space inside the reaction vessel 502, thereby combining with the nucleic acid amplification technology to amplify DNA.

[0038] In summary, the beneficial effects of the present invention are specifically embodied in that, in this isothermal amplification device, the support rod 4 is arranged in the conveying groove 2, and after the ball 402 is limited by the U-shaped block 401 fixed at the end of the support rod 4, the ball 402 can roll freely on the inner peripheral wall of the U-shaped block 401, and the ball 402 is allowed to collide with the inner peripheral wall of the conveying groove 2, and then the support rod 4 can slide in the conveying groove 2 under the action of the ball 402, and because every two support rods 4 can form a group after being connected by the support block 406, and thus form a structure similar to an H-shaped state, so that the tightness between the two support rods 4 in the same group can be increased, so that the four balls 402 at the ends of the two support rods 4 can be in contact with the inner peripheral wall of the conveying groove 2, and then In the first step, the bracket 4 is prevented from tilting. Based on this, the base block 403, the compression spring 404 and the collar 405 are arranged on the outer peripheral wall of the support rod 4, and the base block 403 and the support rod 4 are fixedly connected. Then, the compression spring 404 can use the base block 403 as a force point, so that the collar 405 can have the elasticity of upward displacement under the action of the compression spring 404, thereby driving the support block 406 and other structures fixed thereto to move upward, and then the roller 409 arranged on the top of the support block 406 contacts the guide rail 301 and the U-shaped guide rod 307, so that the support block 406 can move according to the height and trajectory of the guide rail 301 and the U-shaped guide rod 307, and because the roller 409 passes through the circular groove 408 opened between the two triangular blocks 407 The roller 409 is engaged with the guide rail 301, and the roller 409 is engaged with the triangular block 407 and the guide rail 301, thereby indirectly reducing the friction between the triangular block 407 and other structures and the guide rail 301, so that the reaction vessel 502 can move better based on the guide rail 301, and the movement effect tends to be stable. Then, multiple groups of support rods 4 and support blocks 406 are arranged based on this, so that the device can contain multiple groups of components that can perform isothermal amplification of DNA, so that the device can have the function of simultaneously amplifying multiple different DNAs, and because the height of the detection component 6 connected to the top of the support rod 4 is always in a constant state during the movement, and the bottom is supported by the support rod 4. The components connected to the pad 5 connected to the support block 406 can be shifted up and down based on the guide rail 301 and the U-shaped guide rod 307, so that the distance between the pad 5 and the detection component 6 changes, and then based on this, the device amplifies the DNA in the reaction vessel 502, and then when the support block 406 is located at the U-shaped guide rod 307, the distance between the reaction vessel 502 and the detection component 6 is enlarged, so that the device cannot amplify the DNA at this time, and it is convenient for the user to take the reaction vessel at this position to replace different DNAs for the device to perform amplification work, thereby making the amplification work of the device sustainable, and it is convenient for the user to replace different DNAs in the reaction vessel 502 to supply the device for amplification work;

[0039] When the device is in use, the hydraulic rod 3 is fixed to the inner circumferential wall of the conveying groove 2, and the guide rail 301 is fixed to the end of the hydraulic rod 3, and then the height of the guide rail 301 is controlled according to the extension and contraction of the hydraulic rod 3, and the distance between the reaction vessel 502 and the detection component 6 is further controlled by the interference effect between the guide rail 301 and the roller 409, and on this basis, through the buffer grooves 302 opened at both ends of the guide rail 301 and the limit slide 303 that can slide in the buffer groove 302, and the limit slide 303 cannot rotate in the buffer groove 302 due to being limited, and then the two ends of the guide rail 301 are connected by the screw sleeve 304 and the rotating screw 305, and after the rotating screw 305 is rotated by external force, the two screw sleeves 304 connected to it through the threaded relationship can Under this action, a deviation occurs, and then the distance between the two screw sleeves 304 changes, and the limiting slide bar 303 slides accordingly in the buffer groove 302. After the distance changes, the two connecting grooves 306 opened in the two limiting slide bars 303 can make the two ends of the U-shaped guide rod 307 pass through the connecting grooves 306 and move based on the change in the spacing between the two connecting grooves 306 after the U-shaped guide rod 307 is connected to the guide rail 301. That is, after this change, the bottom of the U-shaped guide rod 307 can change synchronously with the height of the guide rail 301, and then the spacing between the two can be adjusted by rotating the rotating screw rod 305 by external force, so that the position here can still be convenient for users to replace DNA after the height of the guide rail 301 is adjusted;

[0040] When the device is in use, the cushion block 5 is connected to the support rod 4 through the support block 406, so that the height of the cushion block 5 can be indirectly controlled according to the guide rail 301 and the U-shaped guide rod 307. Since the detection component 6 is directly fixed to the support rod 4, its height cannot be changed. Based on this, the reaction vessel 502 is placed on the top of the anti-slip wall 501 to prevent the reaction vessel 502 from sliding, and then the piston 602 fixed to the bottom of the detection component 6 is located on the top of the reaction vessel 502 and deviates downward based on the reaction vessel 502. In this process, by providing a sealing ring 503, the piston 602 can always be wrapped around its outer peripheral wall during its movement, which can indirectly improve the sealing effect of the reaction vessel 502 and prevent the DNA therein from being affected by air. At the same time, when the piston 602 moves downward based on the reaction vessel 502, the sliding cover 505 seals the reaction vessel 502 before the piston 602 enters the reaction vessel 502. After the piston 602 contacts the sliding cover 505, since the top of the sliding cover 505 is inclined, the downward pressure applied by the piston 602 to the sliding cover 505 can be converted into lateral pressure, and then the sliding cover 505 moves toward the side opening 504, thereby exposing the bottom space of the sliding cover 505, so that the piston 602 can pressurize the DNA at this position, and on this basis, combined with the nucleic acid amplification technology, the DNA is amplified accordingly, and the auxiliary device amplifies the DNA, and through the action of the sliding cover 505, the DNA can be prevented from being affected by the air, thereby ensuring the accuracy of the detection result.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. An isothermal amplification device, characterized in that: The invention comprises a shell (1), wherein a conveying trough (2) is provided in the shell (1), wherein the inner peripheral wall of the conveying trough (2) is provided with a plurality of supporting rods (4), wherein two ends of the plurality of supporting rods (4) are fixedly connected to two U-shaped blocks (401), wherein the inner peripheral wall of the U-shaped block (401) is rotatably connected to a ball (402), wherein the supporting rod (4) contacts the inner peripheral wall of the conveying trough (2) through the ball (402), wherein the outer peripheral walls of the plurality of supporting rods (4) are fixedly connected to a base block (403), wherein the outer peripheral walls of the plurality of supporting rods (4) are sleeve-connected to a compression spring (404), wherein the outer peripheral walls of the plurality of supporting rods (4) are slidably connected to a sleeve ring (405), wherein the two ends of the compression spring (404) are respectively connected to the base block (403) and the collar (405) are fixedly connected; the outer peripheral wall of the collar (405) is fixedly connected with a support block (406), and every two of the support rods (4) are arranged into a group through one of the support blocks (406), and the support block (406) extends out of the housing (1) through the opening of the conveying trough (2); the top of the support block (406) is fixedly connected with two triangular blocks (407), and the two triangular blocks (407) are provided with circular grooves (408) at opposite positions, and the inner peripheral wall of the circular groove (408) is rotatably connected with a roller (409); the inner peripheral wall of the conveying trough (2) is provided with a plurality of hydraulic rods (3), and the ends of the hydraulic rods (3) are fixedly connected with guide rails (3 01), buffer grooves (302) are provided at both ends of the guide rail (301), and the inner walls of the two buffer grooves (302) are slidably connected to the limit slide rod (303); the ends of the two limit slide rods (303) are fixedly connected to the screw sleeves (304), and the inner walls of the two screw sleeves (304) are threadedly connected to the rotating screw (305); the ends of the two limit slide rods (303) are provided with connecting grooves (306), and the inner walls of the connecting grooves (306) are penetrated and connected with U-shaped guide rods (307), and the guide rail (301) and the U-shaped guide rod (307) are in conflict with the roller (409); one side of the support block (406) is fixedly connected to a cushion block (5), the top of the cushion block (5) is provided with an anti-slip wall (501), the top of the anti-slip wall (501) is provided with a reaction vessel (502), the inner peripheral wall of the reaction vessel (502) is fixedly connected with a sealing ring (503); a side opening (504) is provided on one side of the reaction vessel (502), the inner peripheral wall of the side opening (504) is provided with an elastic sealing block (506), the bottom of the elastic sealing block (506) is slidably connected with a sliding cover (505); the outer peripheral wall of the support rod (4) is fixedly connected with a detection component (6), the bottom of the detection component (6) is fixedly connected with a piston (602), and the piston (602) is located on the inner peripheral wall of the reaction vessel (502);The bottom of the detection component (6) is provided with a built-in groove (601), and the inner diameter of the built-in groove (601) is adapted to the thickness of the reaction vessel (502). ;

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