Auricularia auricular polysaccharide liposome, hydrogel film and preparation method and application thereof

Abstract

The present application relates to the technical field of skin care products, and particularly relates to a tremella aurantialba polysaccharide liposome, a hydrogel film and a preparation method and application thereof. The tremella aurantialba polysaccharide liposome takes phosphorylated tremella aurantialba polysaccharide as a core material, and takes soybean lecithin and cholesterol as wall materials; and the liposome is prepared by using a film hydration method. The tremella aurantialba polysaccharide hydrogel film comprises 10-20 parts of the tremella aurantialba polysaccharide liposome, 11-15 parts of gelatin, 11-15 parts of chitosan, 1.5-2.5 parts of thioctic acid, 5-15 parts of glycerol and 3-5 parts of ergothioneine. The gelatin is added to water and heated and stirred; the chitosan is added to 0.5-2% acetic acid solution and stirred; the mixture is mixed, and then the tremella aurantialba polysaccharide liposome is added and mixed uniformly; the thioctic acid, the glycerol and the ergothioneine are added and magnetically stirred; and then the mixture is laid flat and dried at room temperature for 1-3 days. The advantage lies in that the chitosan and the tremella aurantialba polysaccharide liposome play a double antibacterial role, continuously and efficiently act on the skin, and have anti-inflammatory and acne-removing effects.

Description

Technical Field

[0001] This invention relates to the field of skin care product technology, and in particular to a polysaccharide liposome of Auricularia auricula-judae, a hydrogel film, its preparation method and application. Background Technology

[0002] With rapid socio-economic development, acne has become a common skin problem. Acne, also known as pimples, is a chronic inflammatory skin disease caused by the blockage and inflammation of hair follicles and sebaceous glands. Research shows that the influencing factors and pathogenesis of acne are extremely complex. Major influencing factors include internal microbial imbalance, immune inflammatory response, endocrine disorders, abnormal keratinization of hair follicles and sebaceous gland ducts, and abnormal sebaceous gland secretion. External factors include living environment, mental stress, and improper use of cosmetics. Acne affects appearance, and improper squeezing can cause further inflammation and even bacterial infection.

[0003] Currently, there are many methods for treating acne, such as laser acne treatment, physical acne treatment, traditional Chinese medicine acne treatment, and Western medicine acne treatment. However, these methods all have certain limitations and cannot effectively, safely, or provide a short-term cure. One of the important factors leading to acne development is Propionibacterium acnes. Therefore, developing a product that rapidly inhibits the growth and reproduction of Propionibacterium acnes while being skin-friendly is a problem that needs to be solved in the field of acne treatment technology.

[0004] Salicylic acid, also known as salicylic acid, β-salicylic acid Hydroxybenzoic acid can be extracted from willow bark. Salicylic acid is an extract from willow bark and is a natural anti-inflammatory agent. Salicylic acid can exfoliate, unclog pores, shrink pores, and dissolve acne, thus playing an anti-inflammatory and antipruritic role. Chitosan, chemically known as polyglucosamine (1-4)-2-amino-BD glucose, is a product obtained from chitin, which is widely found in nature, through deacetylation. Chitosan oligosaccharides have significant moisturizing effects, inhibit the growth of harmful bacteria on the skin surface, and have antibacterial and anti-skin disease effects, and can be used in skin care products. A method for preparing and applying an acne-removing and oil-controlling microemulsion gel is disclosed in the invention titled "An Acne-Removing and Oil-Controlling Microemulsion Gel and Its Preparation Method and Application" published on June 15, 2018, under publication number CN108158993A. This invention relates to an acne-fighting and oil-controlling microemulsion gel and its preparation method. The microemulsion gel includes salicylic acid, niacinamide, ascorbic acid, bisabolol, zinc pyrrolidone carboxylate, panthenol, dipotassium glycyrrhizate, an oil phase, surfactants, co-surfactants, a gel matrix material, pH adjusters, and preservatives. While it can achieve certain acne-fighting effects, the excessive addition of ingredients, including preservatives, can easily cause other damage to skin tissue and trigger allergies. Therefore, developing safer skincare and acne-fighting products is essential. Summary of the Invention

[0005] To address the aforementioned problems, this invention provides a polysaccharide liposome from Auricularia auricula-judae, a hydrogel film, its preparation method, and its application.

[0006] The primary objective of this invention is to provide a polysaccharide liposome of Auricularia auricula-judae, with phosphorylated Auricularia auricula-judae polysaccharide as the core material and soybean lecithin and cholesterol as the wall material, wherein the weight ratio of soybean lecithin, cholesterol and phosphorylated Auricularia auricula-judae polysaccharide is 12.5~17.5:2.5~3.5:1; The preparation method of the *Auricularia auricula-judae* polysaccharide liposomes is as follows: weigh soybean lecithin, cholesterol and phosphorylated *Auricularia auricula-judae* polysaccharide according to the weight ratio, dissolve in anhydrous ethanol, and evaporate under reduced pressure by rotary evaporation; then add PBS buffer solution, wash the membrane in a water bath at 32~37℃, and sonicate in a water bath for 10~20 min; dry to obtain *Auricularia auricula-judae* polysaccharide liposomes.

[0007] The preferred method for preparing phosphorylated Auricularia auricula polysaccharide is as follows: Sodium tripolyphosphate and sodium trimetaphosphate were weighed and dissolved in distilled water to prepare a phosphorylation reagent of 65-75 mg / mL. 0.3-1 g of Auricularia auricula polysaccharide and 1.5-5 g of sodium sulfate were added and fully dissolved. The pH was adjusted to 7.5-8.5, and the reaction was carried out at 80-90℃ for 4-6 h. After the reaction was completed, 95% ethanol solution was added, and the mixture was allowed to stand for 20-30 h to precipitate. The ethanol was removed by centrifugation. The mixture was then reconstituted, dialyzed, and concentrated to obtain phosphorylated Auricularia auricula polysaccharide.

[0008] Preferably, the dialysis conditions are 8000 Da for 40-50 h; the centrifugation speed is 4500-5500 r / min for 8-15 min.

[0009] Preferably, the weight ratio of soybean lecithin, cholesterol, and phosphorylated Auricularia auricula polysaccharide is 15:3:1; and the encapsulation rate of the Auricularia auricula polysaccharide liposomes is >80%.

[0010] The second objective of this invention is to provide a polysaccharide hydrogel film for *Auricularia auricula-judae*, comprising the following components in parts by weight: 10-20 parts of the polysaccharide liposomes for *Auricularia auricula-judae*, 11-15 parts of gelatin, 11-15 parts of chitosan, 1.5-2.5 parts of lipoic acid, 5-15 parts of glycerol, and 3-5 parts of ergothioneine.

[0011] Preferably, it is prepared by the following method: S1. Weigh out the following ingredients by weight: Auricularia auricula polysaccharide liposomes, gelatin, chitosan, alpha-lipoic acid, glycerol, and ergothioneine. S2. Add gelatin to water, heat and stir at 40~50℃ to prepare a gelatin aqueous solution; add chitosan to an acetic acid solution with a concentration of 0.5~2%, stir to prepare a chitosan solution; mix the gelatin aqueous solution and the chitosan solution to obtain a gelatin-chitosan mixture; S3. Add the auricularia auricula polysaccharide liposomes to the gelatin-chitosan mixture and mix well; add lipoic acid, glycerol, and ergothioneine, and stir magnetically for 20-40 minutes to obtain a mixed suspension; S4. Spread the mixed suspension evenly and dry it at room temperature for 1-3 days to obtain a polysaccharide hydrogel film of Auricularia auricula-judae.

[0012] Preferably, the concentration of the acetic acid solution is 1%.

[0013] Preferably, the mass ratio of chitosan to gelatin is 1:1.

[0014] The preferred composition is 10 parts of *Auricularia auricula-judae* polysaccharide liposomes, 11 parts of gelatin, 11 parts of chitosan, 1.5 parts of lipoic acid, 5 parts of glycerol, and 3 parts of ergothioneine.

[0015] The third objective of this invention is to provide an application of a polysaccharide hydrogel film from *Auricularia auricula-judae* in the preparation of acne-removing and moisturizing skincare products.

[0016] Compared with the prior art, the present invention can achieve the following beneficial effects: (1) The microporous permeable hydrogel small molecule auricularia auricula polysaccharide hydrogel film preparation method of the present invention is simple to operate, does not add strong alkali neutralizer, greatly reduces the irritation to the skin, the system is stable, and is free of preservatives and alcohol, with simple composition and gentle soothing effect on the skin.

[0017] (2) While the antibacterial effects of chitosan and Auricularia auricula polysaccharide liposomes are exerted, the dual antibacterial effects of chitosan and Auricularia auricula polysaccharide liposomes are also exerted, so that the active molecules can act on the skin continuously and efficiently, effectively exert their anti-inflammatory effects, and help to remove acne. Attached Figure Description

[0018] Figure 1 The results show the effect of the mass ratio of soybean lecithin to cholesterol and the mass ratio of soybean lecithin to Auricularia auricula-judae polysaccharide on the encapsulation efficiency of Auricularia auricula-judae polysaccharide liposomes according to embodiments of the present invention.

[0019] Figure 2 The results show the effect of the soybean lecithin to cholesterol mass ratio and ultrasonic time on the encapsulation efficiency of Auricularia auricula-judae polysaccharide liposomes according to embodiments of the present invention.

[0020] Figure 3 The results show the effect of the mass ratio of soybean lecithin to phosphorylated Auricularia auricula polysaccharide and the ultrasonic time on the encapsulation efficiency of Auricularia auricula polysaccharide liposomes according to embodiments of the present invention.

[0021] Figure 4 This is a transmission electron microscope image of the polysaccharide liposomes of Auricularia auricula-judae provided according to an embodiment of the present invention. Detailed Implementation

[0022] In the following description, embodiments of the invention will be described with reference to the accompanying drawings. In the description below, the same modules are denoted by the same reference numerals. Where the same reference numerals are used, their names and functions are also the same. Therefore, their detailed description will not be repeated.

[0023] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not constitute a limitation thereof.

[0024] Example 1 A method for preparing polysaccharide liposomes from Auricularia auricula-judae includes the following steps: S101. Phosphorylation modification of Auricularia auricula-judae polysaccharide: 5g of sodium tripolyphosphate and 2g of sodium trimetaphosphate were dissolved in distilled water to prepare a 70mg / mL phosphorylation reagent. 0.5g of Auricularia auricula-judae polysaccharide and 2.5g of sodium sulfate were added, and the pH was adjusted to 8 after dissolution. The solutions were reacted at 85℃ for 5h. After the reaction, 95% ethanol solution was added to increase the solution volume to four times the original volume. The solution was allowed to stand for 24h to precipitate, and then centrifuged at 5000r / min for 10min to remove the ethanol. After redissolving, dialyzing (8000Da) for 48h, and then concentrating and lyophilizing, phosphorylated Auricularia auricula-judae polysaccharide was obtained. The monosaccharide composition of phosphorylated Auricularia auricula polysaccharide is arabinose, xylose, D-mannose, galactose, D(+)-mannose, D-glucose and β-glucose, with a molar ratio of 1.05:4.58:5.18:0.34:2.25:0.52:2.11; S102. Soybean lecithin, cholesterol, and phosphorylated *Auricularia auricula-judae* polysaccharide were weighed in a weight ratio of 15:3:1, and *Auricularia auricula-judae* polysaccharide liposome solution was prepared using a thin-film hydration method. Specifically, a certain amount of cholesterol, soybean lecithin, and phosphorylated Auricularia auricula polysaccharide were placed in a flask, 100 mL of anhydrous ethanol was added and dissolved by ultrasonication, the ethanol was removed by rotary evaporation under reduced pressure, an appropriate amount of PBS buffer solution was added to the flask, the membrane was washed in a water bath at 35°C, and ultrasonicated in a water bath for 15 min to obtain the Auricularia auricula polysaccharide liposome solution. S103. Freeze-dry the Auricularia auricula polysaccharide liposome solution into freeze-dried powder to obtain Auricularia auricula polysaccharide liposomes.

[0025] Example 2 A method for preparing a polysaccharide hydrogel film from Auricularia auricula-judae includes the following steps: S1. Weigh the following components in parts by mass: 11 parts gelatin, 11 parts chitosan, 10 parts auricularia auricula polysaccharide liposomes, 1.5 parts lipoic acid, 5 parts glycerol, and 3 parts ergothioneine; S2. Add gelatin to water, heat and stir at 45°C to prepare a gelatin aqueous solution; add chitosan to a 1% acetic acid solution and stir to prepare a chitosan solution; mix the gelatin aqueous solution and the chitosan solution to obtain a gelatin-chitosan mixture. S3. Add 10 parts of Auricularia auricula polysaccharide liposomes to the gelatin-chitosan mixture and mix well; add lipoic acid, glycerol, and ergothioneine, and stir magnetically for 30 minutes to obtain a mixed suspension; S4. The mixed suspension was spread onto a glass slide and dried at room temperature (15~25℃) for 2 days to obtain a *Auricularia auricula-judae* polysaccharide hydrogel film. The film was measured to have a thickness of 49.42±5.61 μm, a water content of 21.09±3.02%, a tensile strength of 7.13±0.12 MPa, and an elongation at break of 33.42±0.11%.

[0026] Example 3 A method for preparing a polysaccharide hydrogel film from Auricularia auricula-judae includes the following steps: S1. Weigh the following components in parts by weight: 13 parts gelatin, 13 parts chitosan, 15 parts auricularia auricula polysaccharide liposomes, 2 parts lipoic acid, 10 parts glycerol, and 4 parts ergothioneine. S2. Add gelatin to water, heat and stir at 45°C to prepare a gelatin aqueous solution; add chitosan to a 1% acetic acid solution and stir to prepare a chitosan solution; mix the gelatin aqueous solution and the chitosan solution to obtain a gelatin-chitosan mixture. S3. Add 10 parts of Auricularia auricula polysaccharide liposomes to the gelatin-chitosan mixture and mix well; add lipoic acid, glycerol, and ergothioneine, and stir magnetically for 30 minutes to obtain a mixed suspension; S4. The mixed suspension was spread onto a glass slide and dried at room temperature (15~25℃) for 2 days to obtain a *Auricularia auricula-judae* polysaccharide hydrogel film. The film was measured to have a thickness of 52.17±3.29 μm, a water content of 26.47±2.16%, a tensile strength of 8.51±0.18 MPa, and an elongation at break of 35.09±0.15%.

[0027] Example 4 A method for preparing a polysaccharide hydrogel film from Auricularia auricula-judae includes the following steps: S1. Weigh the following components in parts by weight: 15 parts gelatin, 15 parts chitosan, 20 parts auricularia auricula polysaccharide liposomes, 2.5 parts lipoic acid, 15 parts glycerol, and 5 parts ergothioneine. S2. Add gelatin to water, heat and stir at 45°C to prepare a gelatin aqueous solution; add chitosan to a 1% acetic acid solution and stir to prepare a chitosan solution; mix the gelatin aqueous solution and the chitosan solution to obtain a gelatin-chitosan mixture. S3. Add 10 parts of Auricularia auricula polysaccharide liposomes to the gelatin-chitosan mixture and mix well; add lipoic acid, glycerol, and ergothioneine, and stir magnetically for 30 minutes to obtain a mixed suspension; S4. The mixed suspension was spread onto a glass slide and dried at room temperature (15~25℃) for 2 days to obtain a *Auricularia auricula-judae* polysaccharide hydrogel film. Its thickness was 56.73±4.28 μm, water content was 29.12±2.93%, tensile strength was 9.06±0.14 MPa, and elongation at break was 37.16±0.13%.

[0028] Comparative Example 1 A method for preparing a chitosan hydrogel film specifically includes the following steps: S1. Weigh the following components in parts by weight: 13 parts gelatin, 13 parts chitosan, 2 parts lipoic acid, 10 parts glycerin, and 4 parts ergothioneine; S2. Add gelatin to water, heat and stir at 45°C to prepare a gelatin aqueous solution; add chitosan to a 1% acetic acid solution, heat and stir to prepare a chitosan solution; mix the gelatin aqueous solution and the chitosan solution to obtain a gelatin-chitosan mixture; S3. Add thioctic acid, glycerol, and ergothioneine to the gelatin-chitosan mixture, and stir magnetically for 30 minutes to obtain a mixed suspension; S4. The mixed suspension was spread onto a glass slide and dried at room temperature (15~25℃) for 2 days to obtain a chitosan hydrogel film. Its thickness was 47.35±3.92 (µm), water content was 19.95±2.17%, tensile strength was 6.85±0.25 (MPa), and elongation at break was 30.37±0.15%.

[0029] Example 5 The optimization experiment of response surface methodology for polysaccharide liposomes from Auricularia auricula-judae was conducted as follows: Response surface methodology was designed to optimize the conditions, using the mass ratio of soybean lecithin and cholesterol to phosphorylated *Auricularia auricula-judae* polysaccharide, and the ultrasonic reaction time of the hydration film method as variables, with the final encapsulation efficiency of *Auricularia auricula-judae* polysaccharide liposomes as the indicator. The optimal conditions were explored. The factors and levels are shown in Table 1 below. Table 1. Optimization Design of Response Surface of Auricularia auricula-judae Polysaccharide Liposomes

[0030] The results of response surface optimization are shown below. Figures 1-3The results show a significant interaction between AB and BC. Through software optimization, using the encapsulation rate of phosphorylated *Auricularia auricula-judae* polysaccharide liposomes as an indicator, the optimal process was modified through validation experiments to a soybean lecithin to cholesterol mass ratio of 5:1, a soybean lecithin to phosphorylated *Auricularia auricula-judae* polysaccharide mass ratio of 15:1, and an ultrasonic time of 15 min. Under these conditions, the encapsulation rate of *Auricularia auricula-judae* polysaccharide liposomes reached 80.10%.

[0031] Transmission electron microscopy (TEM) images of the polysaccharide liposomes prepared under optimized conditions are shown below. Figure 4 Transmission electron microscopy revealed that the polysaccharide liposomes of Auricularia auricula-judae were all spherical, uniform in size, round, and without adhesion, with an average particle size of 181±2.8 nm.

[0032] Example 6 The experiment on the inhibition of Propionibacterium acnes by polysaccharide hydrogel film of Auricularia auricula-judae was conducted as follows: After activating the frozen Propionibacterium acnes strain, the bacterial solution was diluted to 1.5 × 10⁻⁶. 6 CFU / ml. 50 μL of diluted bacterial solution was added to each well of a 96-well plate, along with 50 μL of sample solution, and a blank control was set up. The 96-well plate was incubated in an anaerobic incubator for 2 days, and the inhibition rate of Propionibacterium acnes was measured at 1 h, 12 h, and 24 h; the results are shown in Table 2.

[0033] Table 2. Inhibition results of Auricularia auricula-judae polysaccharide hydrogel film on Propionibacterium acnes

[0034] As shown in Table 2, the *Auricularia auricula-judae* polysaccharide hydrogel film (i.e., acne-removing gel) prepared in this invention has excellent antibacterial effects. It can inhibit the reproduction of *Propionibacterium acnes* within 1 hour and 12 hours; and it can inhibit the growth of most *Propionibacterium acnes* within 24 hours. In contrast, the gel film of Comparative Example 1 without the addition of *Auricularia auricula-judae* polysaccharide liposomes only showed an inhibition rate of 51.7% against *Propionibacterium acnes* within 24 hours, which is far less effective than the gel film prepared in this invention.

[0035] Example 7 The *Auricularia auricula-judae* polysaccharide hydrogel films prepared in Examples 2-4 and Comparative Example 1 were directly applied to the human body to test their acne-removing efficacy. The specific testing method was as follows: Sixty acne patients aged 18-40 years (30 males and 30 females) were selected and randomly divided into four groups. The gel film prepared in Examples 2-4 and Comparative Example 1 was applied twice daily to the affected facial areas. The test period was 30 days, during which no other medications or skincare products were used. The number of facial lesions (pustules, pimples, etc.) was counted in each of the four groups, and the efficacy was calculated: (number of lesions before use) (Number of skin lesions after use) / Number of skin lesions before use × 100%. A cure rate greater than 95% is considered basically cured, a cure rate of 60-94% is considered significantly effective, and a cure rate below 60% is considered ineffective. Test results are shown in Table 3.

[0036] Table 3. Results of the Acne-Removing Efficacy Test of Auricularia auricula-judae Polysaccharide Hydrogel Film

[0037] Table 3 shows that when the *Auricularia auricula-judae* polysaccharide hydrogel films prepared in Examples 2-4 were applied to human acne treatment, the number of ineffective cases after one week of use was 0, indicating that the *Auricularia auricula-judae* polysaccharide hydrogel film of the present invention has a rapid onset of action. After 30 days of use, at least 7 people achieved basic recovery, demonstrating the excellent acne treatment effect of the gel of the present invention. In contrast, Comparative Example 1, without the addition of *Auricularia auricula-judae* polysaccharide liposomes, only had 4 people achieving basic recovery after 30 days of use.

[0038] The hydrogel film of this invention is mainly composed of a gelatin-chitosan complex, a natural solution with antibacterial properties. Thioctic acid provides superficial exfoliation, focusing on the prevention and treatment of excessive oil production, closed comedones, and acne (blackheads and whiteheads); *Auricularia auricula-judae* polysaccharide has anti-inflammatory properties, focusing on the prevention and treatment of inflammatory, red, and swollen acne and the removal of acne scars; glycerin and ergothione promote the synthesis of collagen and other proteins in the skin, thereby achieving moisturizing and soothing effects. Repairing effect; the six ingredients, in a specific compound ratio, work synergistically to achieve excellent acne-removing effects. It is fruit-based, preservative-free, and has a simple formula. The ingredients used are gentle on the skin, mild, and safe.

[0039] It should be understood that the various forms of processes shown above can be used to reorder, add, or delete steps. For example, the steps described in this invention disclosure can be executed in parallel, sequentially, or in different orders, as long as the desired result of the technical solution disclosed in this invention can be achieved, and this is not limited herein.

[0040] The specific embodiments described above do not constitute a limitation on the scope of protection of this invention. Those skilled in the art should understand that various modifications, combinations, sub-combinations, and substitutions can be made according to design requirements and other factors. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. The application of a polysaccharide hydrogel film from *Auricularia auricula-judae* in the preparation of acne-removing and moisturizing skincare products, characterized in that: The *Auricularia auricula-judae* polysaccharide hydrogel film comprises the following components in parts by weight: 10-20 parts of *Auricularia auricula-judae* polysaccharide liposomes, 11-15 parts of gelatin, 11-15 parts of chitosan, 1.5-2.5 parts of lipoic acid, 5-15 parts of glycerol, and 3-5 parts of ergothioneine; the mass ratio of chitosan to gelatin is 1:

1. Prepared by the following method: S1. Weigh out the following ingredients by weight: Auricularia auricula polysaccharide liposomes, gelatin, chitosan, alpha-lipoic acid, glycerol, and ergothioneine. S2. Add gelatin to water, heat and stir at 40~50℃ to prepare a gelatin aqueous solution; Chitosan was added to a 1% acetic acid solution and stirred to prepare a chitosan solution; the gelatin aqueous solution and the chitosan solution were mixed to obtain a gelatin-chitosan mixture. S3. Add the auricularia auricula polysaccharide liposomes to the gelatin-chitosan mixture and mix well; add lipoic acid, glycerol, and ergothioneine, and stir magnetically for 20-40 minutes to obtain a mixed suspension; S4. Spread the mixed suspension evenly and dry it at room temperature for 1-3 days to obtain a polysaccharide hydrogel film from Auricularia auricula-judae. The *Auricularia auricula-judae* polysaccharide liposomes use phosphorylated *Auricularia auricula-judae* polysaccharide as the core material and soybean lecithin and cholesterol as the wall material. The weight ratio of soybean lecithin, cholesterol and phosphorylated *Auricularia auricula-judae* polysaccharide is 15:3:

1. The average particle size of the *Auricularia auricula-judae* polysaccharide liposomes is 181±2.8 nm. The preparation method of the *Auricularia auricula-judae* polysaccharide liposomes is as follows: soybean lecithin, cholesterol and phosphorylated *Auricularia auricula-judae* polysaccharide are weighed according to the weight ratio, dissolved in anhydrous ethanol, and evaporated under reduced pressure by rotary evaporation; then PBS buffer solution is added, the membrane is washed in a water bath at 32~37℃, and ultrasonicated in a water bath for 10~20 min; dried, and the *Auricularia auricula-judae* polysaccharide liposomes are obtained. The preparation method of the phosphorylated Auricularia auricula polysaccharide is as follows: Sodium tripolyphosphate and sodium trimetaphosphate are weighed and dissolved in distilled water to prepare a phosphorylation reagent of 65-75 mg / mL; 0.3-1 g of Auricularia auricula polysaccharide and 1.5-5 g of sodium sulfate are added, fully dissolved, and the pH value is adjusted to 7.5-8.

5. The reaction is carried out at 80-90℃ for 4-6 h; after the reaction is completed, 95% ethanol solution is added, and the mixture is allowed to stand for precipitation for 20-30 h. The mixture is then centrifuged at 4500-5500 r / min for 8-15 min to remove the ethanol. The phosphorylated Auricularia auricula polysaccharide was obtained by redissolving, dialysis, and concentration.

2. The application of the *Auricularia auricula-judae* polysaccharide hydrogel film according to claim 1 in the preparation of acne-removing and moisturizing skin care products, characterized in that: The dialysis conditions were 8000 Da for 40-50 hours.

3. The application of the *Auricularia auricula-judae* polysaccharide hydrogel film according to any one of claims 1-2 in the preparation of acne-removing and moisturizing skin care products, characterized in that: The encapsulation rate of the *Auricularia auricula-judae* polysaccharide liposomes is >80%.

4. The application of the *Auricularia auricula-judae* polysaccharide hydrogel film according to claim 1 in the preparation of acne-removing and moisturizing skin care products, characterized in that: The composition of the auricularia auricula polysaccharide liposome is 10 parts, gelatin 11 parts, chitosan 11 parts, thioctic acid 1.5 parts, glycerol 5 parts, and ergothioneine 3 parts.

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