Reaction cup, reaction liquid mixing method and biochemical analyzer

Abstract

This application relates to the field of biochemical analysis equipment technology, disclosing a reaction cup, a method for mixing the reaction solution, and a biochemical analyzer. The reaction cup has an opening at the top, and the inner wall of the bottom of the cup forms at least one inclined structure. The inclined structure forms an angle with the inner wall and / or side wall of the bottom of the cup, causing the area of ​​the inner cavity at the bottom of the cup to gradually decrease from the top to the bottom. This application's embodiment allows for the mixing of the sample and reagents without a stirring rod, eliminating cross-contamination caused by the stirring rod. Furthermore, the inclined surface of the reaction cup facilitates the removal of air bubbles from the mixture and reduces the amount of both the sample and reagents used, improving the accuracy and reliability of the detection results.

Description

Technical Field

[0001] This application relates to the field of biochemical analysis equipment technology, and in particular to a reaction cup, a method for mixing reaction solution, and a biochemical analyzer. Background Technology

[0002] When a biochemical analyzer is in operation, reagents and samples need to be added to a reaction vessel to ensure that the reagents and samples are thoroughly mixed before the reaction is carried out and the reaction results are tested.

[0003] In the specific mixing process, a motor is usually used to drive a stirring rod to rotate in the reaction liquid. However, since the reaction cup is a reusable device, the mixing process using a motor-driven stirring rod requires high cleaning standards. Otherwise, cross-contamination can easily occur, which may lead to unstable test results. Summary of the Invention

[0004] The purpose of this application is to provide a reaction cup, a method for mixing reaction solution, and a biochemical analyzer, which can avoid contamination of reagents and samples by residual substances on the stirring rod, thereby improving the accuracy and reliability of the detection results.

[0005] This application provides a reaction cup with an opening at the top and at least one inclined structure formed on the inner wall of the bottom. The inclined structure forms an angle with the inner wall and / or side wall of the bottom of the reaction cup, so that the area of ​​the inner cavity of the bottom of the reaction cup gradually decreases from the top to the bottom.

[0006] In some embodiments, the inner wall of the bottom of the reaction cup forms a sloped structure, one end of which is connected to the side wall of the reaction cup, and the other end of which is connected to the inner wall of the bottom of the reaction cup.

[0007] In some embodiments, the inner wall of the bottom of the reaction cup forms two inclined structures, which are arranged opposite to each other. One end of the inclined structure is connected to the side wall of the reaction cup, and the other end of the inclined structure is connected to the inner wall of the bottom of the reaction cup.

[0008] In some embodiments, the inner wall of the bottom of the reaction vessel forms two inclined structures, which are arranged adjacent to each other, with one end of each inclined structure connected to the other, and the other end of each inclined structure connected to the inner wall of the bottom of the reaction vessel.

[0009] In some embodiments, the interior angle formed between the inclined structure and the sidewall of the reaction vessel is [100°, 170°]; and / or the interior angle formed between the inclined structure and the inner wall of the bottom of the reaction vessel is [100°, 170°].

[0010] This application embodiment also provides a reaction solution mixing method applied to the above-mentioned reaction vessel, the reaction solution mixing method comprising: Perform a first pipetting operation; the first pipetting operation is to transfer a reagent or a sample to be tested into a reaction vessel that does not contain liquid; After the first pipetting operation is completed, the second pipetting operation is performed; when the first pipetting operation is to transfer a reagent to a reaction vessel, the second pipetting operation is to transfer a sample to be tested to the reaction vessel; when the first pipetting operation is to transfer a sample to be tested to a reaction vessel, the second pipetting operation is to transfer a reagent to the reaction vessel. After the second pipetting operation is completed, the reaction vessel is driven to rotate in a circumferential direction; The absorbance of the liquid in the reaction vessel was measured to obtain the sample analysis results.

[0011] In some embodiments, the method for mixing the reaction solution further includes: After the second pipetting operation is completed, the rotation of the reaction vessel is paused, and the third pipetting operation is performed. After the third pipetting operation is completed, the reaction vessel is rotated again in the circumferential direction. The third pipetting operation is to transfer another reagent into the reaction vessel.

[0012] In some embodiments, the method for mixing the reaction solution further includes: Determine whether the reaction vessel containing the reagent and the sample to be tested has undergone a preset number of rotation cycles; the rotation cycle is defined as the reaction vessel completing one accelerated rotation, one uniform rotation, and one decelerated rotation in sequence; If not, select another reaction vessel that does not contain liquid; return to the previous step to perform the first pipetting operation; If so, output the sample analysis results after the reaction vessel has undergone a preset number of rotation cycles.

[0013] In some embodiments, the linear velocity of the uniformly rotating reaction cup is [600 mm / s, 800 mm / s], the duration of the acceleration and deceleration of the reaction cup is (0, 50 ms], and the volume of the liquid in the reaction cup is [6.67%, 23.33%] of the volume of the reaction cup.

[0014] This application also provides a biochemical analyzer, including the reaction cup described above.

[0015] The beneficial effects of this application are as follows: By forming at least one inclined structure on the inner wall of the bottom of the reaction cup, the inner cavity of the bottom of the reaction cup is narrower at the bottom and wider at the top. The area of ​​the inner cavity of the bottom of the reaction cup gradually decreases from the top to the bottom. The mixture of reagent and sample can be tumbled up along the inclined structure under inertia to a height greater than that tumbled up along the vertical wall. This increases the tumbling amplitude of the mixture of reagent and sample in the inner cavity of the reaction cup, allowing for more uniform mixing of reagent and sample without contact with them. It also avoids contamination of reagents and samples by residual substances on the stirring rod. Furthermore, the presence of the inclined surface of the reaction cup facilitates the removal of air bubbles in the mixture and reduces the amount of both sample and reagent required, thereby improving the accuracy and reliability of the detection results. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of the reaction cup provided in the first embodiment of this application.

[0017] Figure 2 This application Figure 1 A schematic diagram of the planar structure of the reaction vessel provided in the embodiment.

[0018] Figure 3 This is a schematic diagram of the planar structure of the reaction cup provided in the second embodiment of this application.

[0019] Figure 4 This is a schematic diagram of the planar structure of the reaction cup provided in the third embodiment of this application.

[0020] Figure 5 This is a flowchart of the reaction solution mixing method provided in the embodiments of this application.

[0021] Figure 6 This is a schematic diagram of the process of mixing reagents and test samples provided in the embodiments of this application.

[0022] Figure 7 This is a schematic diagram of the sample analysis results provided in the embodiments of this application. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0024] It should be noted that although functional modules are divided in the device schematic diagram and a logical order is shown in the flowchart, in some cases, the steps shown may be performed in a different order than the module division in the device or the order in the flowchart. The terms "first," "second," etc., in the specification, claims, and drawings are used to distinguish similar objects and are not used to describe a specific order or sequence.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing embodiments of this application only and is not intended to limit this application.

[0026] This application provides a reaction cup.

[0027] See also Figures 1 to 4 The reaction cup has an opening 1 at the top and at least one inclined structure 2 formed on the inner wall 3 of the bottom of the reaction cup. The inclined structure 2 forms an angle with the inner wall 3 and / or side wall 4 of the bottom of the reaction cup, so that the area of ​​the inner cavity of the bottom of the reaction cup gradually decreases from the top to the bottom.

[0028] The reaction cup provided in this application embodiment forms at least one inclined structure 2 on the inner wall 3 of the bottom of the reaction cup. The inclined structure 2 can form an angle with both the inner wall 3 of the bottom of the reaction cup and the side wall 4 of the side wall 4 of the reaction cup, and tilt from the side wall 4 of the reaction cup to the inner wall 3 of the bottom of the cup. Alternatively, it can form an angle only with the inner wall 3 of the bottom of the reaction cup, and tilt upward from the inner wall 3 of the bottom of the reaction cup. This makes the bottom cavity of the reaction cup narrower at the bottom and wider at the top, and the area of ​​the bottom cavity of the reaction cup gradually decreases from the top of the cup to the bottom of the cup.

[0029] In practical applications, after the reagents stored in the reagent storage device and the sample to be tested stored in the sample storage device are transferred to the reaction cup, the reaction cup is driven to rotate in a circumferential direction. Utilizing the inertia of the rotation's start and end phases, the mixture of reagents and sample inside the reaction cup tumbles. Because the bottom inner wall 3 of the reaction cup forms at least one inclined structure 2, the height the mixture of reagents and sample tumbles along this inclined structure 2 can exceed the height it tumbles along the other side wall 4 of the reaction cup. This increases the tumbling amplitude of the mixture within the reaction cup's cavity, resulting in a more uniform mixing of the reagents and sample. Simultaneously, because the bottom inner cavity area of ​​the reaction cup gradually decreases from top to bottom, the reaction cup provided in this embodiment presents a higher liquid level when holding the same volume of liquid compared to other reaction containers, facilitating absorbance detection and saving reagent usage.

[0030] See also Figure 1 and Figure 2In one specific embodiment, the inner wall 3 of the bottom of the reaction cup forms a sloping structure 2. One end of the sloping structure 2 is connected to the side wall 4 of the reaction cup, and the other end of the sloping structure 2 is connected to the inner wall 3 of the bottom of the reaction cup. In this embodiment, there is one sloping structure 2, which is formed between the side wall 4 and the inner wall 3 of the bottom of the reaction cup. The sloping structure 2 of the inner wall 3 of the bottom of the reaction cup extends from the inner wall 3 of the bottom of the reaction cup to the side wall 4 of the reaction cup, forming an angle with the inner wall 3 and the side wall 4 of the bottom of the reaction cup, respectively. The other side of the reaction cup is a flat side wall 4, making the inner cavity of the bottom of the reaction cup narrower at the bottom and wider at the top, and the area of ​​the inner cavity of the bottom of the cup gradually decreases from the top of the cup to the bottom of the cup. The interior angle formed between the inclined structure 2 and the side wall 4 of the reaction cup is [100°, 170°], and the interior angle formed between the inclined structure 2 and the inner wall 3 of the bottom of the reaction cup is [100°, 170°]. This allows the mixture of reagent and sample to be tested to be flipped up to a more ideal height along the inclined structure 2 formed at the bottom of the reaction cup 110.

[0031] See Figure 3 In one specific embodiment, the inner wall 3 of the bottom of the reaction cup forms two inclined structures 2, which are arranged opposite to each other. One end of the inclined structure 2 is connected to the side wall 4 of the reaction cup, and the other end of the inclined structure 2 is connected to the inner wall 3 of the bottom of the reaction cup. In this embodiment, there are two inclined structures 2, which are formed between the side wall 4 and the inner wall 3 of the bottom of the reaction cup. The inclined structures 2 of the inner wall 3 of the bottom of the reaction cup extend from the inner wall 3 of the bottom of the reaction cup to the two side walls 4 of the reaction cup, forming angles with the inner wall 3 of the bottom of the reaction cup and the corresponding side wall 4, respectively, so that the inner cavity of the bottom of the reaction cup is narrow at the bottom and wide at the top and symmetrical from left to right. The interior angle formed between the inclined structure 2 and the side wall 4 of the reaction cup is [100°, 170°], and the interior angle formed between the inclined structure 2 and the inner wall 3 of the bottom of the reaction cup is [100°, 170°].

[0032] See Figure 4 In one specific embodiment, the inner wall 3 of the bottom of the reaction vessel forms two inclined structures 2, which are arranged adjacent to each other. One end of the two inclined structures 2 is connected to each other, and the other end of each inclined structure 2 is connected to the inner wall 3 of the bottom of the reaction vessel. In this embodiment, there are two inclined structures 2. One end of each inclined structure 2 is connected to the inner wall 3 of the bottom of the reaction vessel, and the other end of each inclined structure 2 is connected to each other, so that a concave structure is formed in the middle of the inner cavity of the bottom of the reaction vessel. The interior angle formed between the inclined structure 2 and the inner wall 3 of the bottom of the reaction vessel is [100°, 170°].

[0033] Preferably, the interior angle between the shoe upper structure and the side wall 4 and / or the inner wall 3 of the reaction cup is 135°.

[0034] This application also provides a method for mixing the reaction solution, applied to the reaction cup described above.

[0035] Please see Figure 5 , Figure 5 This is a flowchart of the reaction solution mixing method provided in the embodiments of this application. In some embodiments of this application, Figure 5 The method described below may include, but is not limited to, steps S501 to S504. Figure 5 These four steps will be explained in detail.

[0036] Step S501: Perform the first pipetting operation. The first pipetting operation involves transferring a reagent or a sample to be tested into a reaction vessel that does not contain any liquid.

[0037] Step S502: After the first pipetting operation is completed, a second pipetting operation is performed. Specifically, the first pipetting operation involves transferring a reagent to a reaction vessel, and the second pipetting operation involves transferring a sample to be tested to the same reaction vessel.

[0038] Step S503: After the second pipetting operation is completed, the reaction vessel is driven to rotate in a circumferential direction.

[0039] Step S504: Measure the absorbance of the liquid in the reaction vessel to obtain the sample analysis results.

[0040] In this embodiment, after pre-selecting the reagent and the sample to be tested, a first pipetting operation is performed to transfer one of the reagent and the sample to a reaction vessel that does not contain liquid. Then, a second pipetting operation is performed to transfer the other of the reagent and the sample to the reaction vessel. When the reaction vessel contains the reagent and the sample, it is rotated circumferentially to mix the reagent and the sample. By detecting the absorbance of the liquid in the reaction vessel, sample analysis results that characterize the reaction process of the reagent and the sample are obtained. Therefore, the reaction liquid mixing method provided in this embodiment can achieve uniform mixing of the reagent and the sample without contacting the mixture, improving the accuracy and reliability of the detection results.

[0041] In one specific embodiment, the reaction solution mixing method further includes: After the second pipetting operation is completed, pause the rotation of the reaction vessel and perform the third pipetting operation. After the third pipetting operation is completed, rotate the reaction vessel again in a circumferential direction. The third pipetting operation involves transferring another reagent into the reaction vessel.

[0042] In this embodiment, it is applicable to tests requiring the use of two reagents. After the second pipetting operation is completed, the reaction vessel is rotated circumferentially to mix the reagent and the sample. After rotating the reaction vessel for a period of time, the rotation is paused, and a third pipetting operation is performed to add the second reagent to the reaction vessel. After the third pipetting operation is completed, the reaction vessel is rotated circumferentially again to mix the reagent and the sample. Therefore, the reaction solution mixing method provided in this embodiment can ensure that the sample and both reagents are thoroughly mixed when performing tests requiring the use of two reagents. It also allows for simultaneous cleaning of the apparatus for transferring reagents or samples during the first rotation of the reaction vessel, saving testing time.

[0043] In one specific embodiment, the reaction solution mixing method further includes: Determine whether the reaction vessel containing the added reagent and sample has undergone a preset number of rotation cycles. A rotation cycle is defined as the reaction vessel completing one accelerated rotation, one uniform rotation, and one decelerated rotation.

[0044] If not, select another reaction vessel that does not contain liquid. Return to step S501.

[0045] If so, output the sample analysis results after the reaction vessel has undergone a preset number of rotation cycles.

[0046] In this embodiment, when the reaction cup is driven to rotate for several rotation cycles, if the reaction cup containing the reagent and the sample to be tested undergoes a preset number of rotation cycles following the rotation of the reaction disk, the reagent and the sample to be tested in the reaction cup are fully mixed, and the sample analysis result after the reaction cup has undergone the preset number of rotation cycles is output as the final sample analysis result. If the reaction cup containing the reagent and the sample to be tested has not undergone the preset number of rotation cycles, the reagent and the sample to be tested in the reaction cup are not fully mixed, and the reaction cup is driven to rotate in the circumferential direction. At this time, another reaction cup without liquid can be selected, and the reagent and the sample to be tested can be added to the other reaction cup without liquid in sequence, so that multiple reaction cups undergo several rotation cycles simultaneously. Thus, by using the reaction liquid mixing method provided in this embodiment, the mixture of reagent and sample to be tested in the reaction cup undergoes the inertial effect of the start and end phases of the reaction cup rotation several times, so that the reagent and the sample to be tested are mixed evenly. At the same time, multiple reaction cups can undergo several rotation cycles simultaneously, which can save testing time.

[0047] See Figure 6In one specific embodiment, before the rotation cycle begins, the liquid level of the mixture of reagent and sample in the reaction vessel remains horizontal. During the accelerated rotation phase of the rotation cycle, the mixture of reagent and sample in the reaction vessel churns up along the side wall of the reaction vessel without a slope structure due to inertia. During the uniform rotation phase of the rotation cycle, the churning amplitude of the mixture of reagent and sample in the reaction vessel gradually decreases until the liquid level remains horizontal. During the decelerated rotation phase of the rotation cycle, the mixture of reagent and sample in the reaction vessel churns up along the side wall of the reaction vessel with a slope structure due to inertia. The height of the mixture of reagent and sample churning up along this slope structure can exceed the height of churning up along the other side wall of the reaction vessel. After the rotation cycle ends, the churning amplitude of the mixture of reagent and sample in the reaction vessel gradually decreases until the liquid level remains horizontal. Figure 7 As shown, the mixing method in this embodiment produces a smooth reaction curve for the sample analysis results without obvious jump points, which can effectively avoid the serious impact of reagent bubbles on the sample analysis results.

[0048] In one specific embodiment, the linear velocity of the reaction cup rotating at a constant speed is [600 mm / s, 800 mm / s], the duration of acceleration and deceleration of the reaction cup is (0, 50 ms], and the volume of the liquid in the reaction cup is [6.67%, 23.33%] of the volume of the reaction cup. This can avoid the risk of liquid overflow caused by the combination of factors such as inertia, rotational speed and acceleration during rotation.

[0049] This application also provides a biochemical analyzer.

[0050] The biochemical analyzer includes the reaction cup described above. The specific structure of the reaction cup is as described in the above embodiments. Since the biochemical analyzer provided in this application adopts the technical solutions of all the above embodiments, it has at least all the beneficial effects brought about by the technical solutions of the above embodiments, which will not be described in detail here.

[0051] In summary, the reaction cup, reaction solution mixing method, and biochemical analyzer provided in this application form at least one inclined structure on the inner wall of the bottom of the reaction cup, making the bottom cavity of the reaction cup narrower at the bottom and wider at the top. The area of ​​the bottom cavity of the reaction cup gradually decreases from the top to the bottom. The mixture of reagents and test samples can be tumbled up along the inclined structure under inertia to a height greater than that tumbled up along the vertical wall, increasing the tumbling amplitude of the mixture of reagents and test samples in the inner cavity of the reaction cup. This allows for more uniform mixing of reagents and test samples without contact with them, avoiding contamination of reagents and samples by residual substances on the stirring rod. Furthermore, the inclined surface of the reaction cup facilitates the removal of air bubbles in the mixture and reduces the amount of both test sample and reagent required, thereby improving the accuracy and reliability of the detection results.

[0052] It should be understood that in this application, "at least one (item)" means one or more, and "more than" means two or more. "And / or" is used to describe the relationship between related objects, indicating that three relationships can exist. For example, "A and / or B" can represent three cases: only A exists, only B exists, and both A and B exist simultaneously, where A and B can be singular or plural. The character " / " generally indicates that the preceding and following related objects are in an "or" relationship. "At least one (item) of the following" or similar expressions refer to any combination of these items, including any combination of single or plural items. For example, at least one (item) of a, b, or c can represent: a, b, c, "a and b", "a and c", "b and c", or "a and b and c", where a, b, and c can be single or multiple.

[0053] The preferred embodiments of the present application have been described above with reference to the accompanying drawings, but this does not limit the scope of the claims of the present application. Any modifications, equivalent substitutions, and improvements made by those skilled in the art without departing from the scope and substance of the embodiments of the present application shall be within the scope of the claims of the present application.

Claims

1. A method for mixing a reaction solution, characterized in that, include: Perform a first pipetting operation; the first pipetting operation is to transfer a reagent or a sample to be tested into a reaction cup that does not contain liquid; the reaction cup has an opening at the top, and the inner wall of the bottom of the reaction cup forms one or two inclined structures, the inclined structures forming an angle with the inner wall and / or side wall of the bottom of the reaction cup, so that the area of ​​the inner cavity of the bottom of the reaction cup gradually decreases from the top to the bottom; when there is one inclined structure, the inclined structure is formed between the side wall and the inner wall of the bottom of the reaction cup, and the other side of the reaction cup is a flat side wall; when there are two inclined structures, the inner cavity of the bottom of the reaction cup is narrow at the bottom and wide at the top and symmetrical from left to right, or a concave structure is formed in the middle of the inner cavity of the bottom of the reaction cup. After the first pipetting operation is completed, the second pipetting operation is performed; when the first pipetting operation is to transfer a reagent to a reaction vessel, the second pipetting operation is to transfer a sample to be tested to the reaction vessel; when the first pipetting operation is to transfer a sample to be tested to a reaction vessel, the second pipetting operation is to transfer a reagent to the reaction vessel. After the second pipetting operation is completed, the reaction cup is driven to rotate in a circular direction, so that the mixture of reagent and sample to be tested in the reaction cup undergoes the inertial action of the start and end phases of the rotation of the reaction cup several times; while driving the reaction cup to rotate in a circular direction, the reaction cup follows the rotation of the reaction disk and undergoes a preset number of rotation cycles; Determine whether the reaction vessel containing the reagent and the sample to be tested has undergone a preset number of rotation cycles; the rotation cycle is defined as the reaction vessel completing one accelerated rotation, one uniform rotation, and one decelerated rotation in sequence; If not, select another reaction vessel that does not contain liquid; return to the previous step to perform the first pipetting operation; If so, output the sample analysis results after the reaction vessel has undergone a preset number of rotation cycles; The absorbance of the liquid in the reaction vessel was measured to obtain the sample analysis results. The interior angle formed between the inclined structure and the side wall of the reaction vessel is [100°, 170°]; and / or the interior angle formed between the inclined structure and the inner wall of the bottom of the reaction vessel is [100°, 170°]; The linear velocity of the uniformly rotating reaction cup is [600 mm / s, 800 mm / s], the duration of acceleration and deceleration of the reaction cup is (0, 50 ms], and the volume of the liquid in the reaction cup is [6.67%, 23.33%] of the volume of the reaction cup.

2. The method for mixing the reaction solution according to claim 1, characterized in that, The inner wall of the bottom of the reaction vessel forms a sloping structure, one end of which is connected to the side wall of the reaction vessel, and the other end of which is connected to the inner wall of the bottom of the reaction vessel.

3. The method for mixing the reaction solution according to claim 1, characterized in that, The inner wall of the bottom of the reaction vessel forms two inclined structures, which are arranged opposite to each other. One end of the inclined structure is connected to the side wall of the reaction vessel, and the other end of the inclined structure is connected to the inner wall of the bottom of the reaction vessel.

4. The method for mixing the reaction solution according to claim 1, characterized in that, The inner wall of the bottom of the reaction vessel forms two inclined structures, which are arranged adjacent to each other. One end of the two inclined structures is connected to each other, and the other end of the two inclined structures is connected to the inner wall of the bottom of the reaction vessel.

5. The method for mixing the reaction solution according to claim 1, characterized in that, Also includes: After the second pipetting operation is completed, the rotation of the reaction vessel is paused, and the third pipetting operation is performed. After the third pipetting operation is completed, the reaction vessel is rotated again in the circumferential direction. The third pipetting operation is to transfer another reagent into the reaction vessel.

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