Preparation method of acetylglucomannan solution and acetylation degree 1 H NMR measurement method

Abstract

The present invention belongs to the technical field of determining the acetylation degree of acetylglucomannan. 1 The HNMR method cannot accurately determine the acetylglucomannan with low acetylation degree. The present invention provides a method for preparing an acetylglucomannan solution and a method for measuring the acetylation degree. 1 The H NMR determination method comprises the following steps: firstly preparing a dried acetylglucomannan sample and controlling the moisture content of the dried acetylglucomannan sample to be below 5%; preparing an alkaline solution with an alkali concentration of 0.3-5.0 mol / L; rapidly pouring the alkaline solution into the dried acetylglucomannan, starting stirring before or at the moment of pouring the alkaline solution, and controlling the stirring speed to be above 800 rpm, so that the acetylglucomannan is dissolved in the alkaline solution in a very short time to obtain an acetylglucomannan solution; using a NaOD solution as the alkaline solution to prepare the acetylglucomannan solution, which can be used for 1 The HNMR method is used to determine the degree of acetylation, and the determination process is rapid and the results are accurate.

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