A kind of aquatic organism eDNA sample automatic collection device and method

Abstract

The present invention relates to the technical field of aquatic ecological analysis, and discloses an automatic eDNA sample collection device and method for aquatic organisms, comprising a shell, one end of the shell is fixedly connected to a support shell, one side of the support shell is provided with a support assembly, the support assembly comprises a plurality of fixed rods fixedly mounted on the support shell, one end of the fixed rod is fixedly connected to a fixed disk, one side of the fixed disk is fixedly connected to a hand-held handle, and the other side of the fixed disk is provided with a hydraulic rod; a storage assembly is provided inside the shell, the storage assembly comprises a plurality of storage tubes, the storage tubes are fixedly connected to the inside of one end of the shell, and one end of the storage tubes is provided with a liquid inlet hole; a suction assembly is provided between the storage tubes, and one end of the storage tubes is provided with a control assembly. The device can collect liquid samples of different depths during use, does not require repeated operations, and is easy to operate.

Description

Technical Field

[0001] The present invention relates to the technical field of aquatic ecological analysis, and in particular to an automatic collection device and method for eDNA samples of aquatic organisms. Background Art

[0002] In recent years, with the rapid development of molecular biology and ecology, eDNA technology has gradually been applied to the monitoring of aquatic organisms. As an important part of eDNA technology, the research and development and application of aquatic organism eDNA sample collection devices have also made significant progress. At present, there are many types of aquatic organism eDNA sample collection devices, such as portable eDNA samplers, backpack eDNA automatic samplers, etc. With the increasing demand for aquatic ecosystem protection and biodiversity monitoring, traditional biological monitoring methods have been difficult to meet actual needs. Traditional sampling methods usually require a lot of manpower and material resources, and the sampling process is prone to damage the ecological environment. Therefore, it is necessary to develop an efficient, convenient and environmentally friendly sampling device to meet these needs.

[0003] The existing automatic eDNA sample collection device for aquatic organisms often needs to use the device multiple times to collect liquids at different depths on the same water surface. This method not only delays the collection time, but is also prone to errors during operation, affecting the accuracy of the sample. Therefore, it does not meet the existing needs. In this regard, we propose an automatic eDNA sample collection device for aquatic organisms. Summary of the invention

[0004] The present invention provides an automatic eDNA sample collection device and method for aquatic organisms, which has the beneficial effect of being able to collect multiple liquid samples at different depths in one use, solving the problem mentioned in the above background technology that when sampling and collecting liquids at different depths on the same water surface, it is often necessary to use the device multiple times to collect the liquid, which not only delays the collection time, but is also prone to errors during operation, affecting the accuracy of the sample.

[0005] The present invention provides the following technical solution: an automatic eDNA sample collection device for aquatic organisms, comprising a shell, one end of the shell is fixedly connected to a support shell, one side of the support shell is provided with a support assembly, the support assembly comprises a plurality of fixed rods fixedly mounted on the support shell, one end of the fixed rods is fixedly connected to a fixed plate for maintaining stability, one side of the fixed plate is fixedly connected to a handheld handle for user control, and the other side of the fixed plate is provided with a hydraulic rod for providing power;

[0006] A storage assembly is disposed inside the housing, and the storage assembly includes a plurality of storage tubes for storing liquids, the storage tubes are fixedly connected to the interior of one end of the housing, and one end of the storage tubes is provided with a liquid inlet hole for facilitating the inflow of liquids;

[0007] A suction component is arranged between the storage tubes, and the suction component is used to suck the liquid to be detected. A control component is arranged at one end of the storage tube, and the control component is used to control the flow of the liquid.

[0008] As an optional solution of the automatic eDNA sample collection device for aquatic organisms described in the present invention, the storage component also includes a temporary storage tray fixedly installed inside the outer shell, the temporary storage tray is fixedly installed inside one end of the outer shell, the temporary storage tray is used to provide a temporary storage space for liquid, one side of the temporary storage tray is connected to a liquid inlet pipe, the liquid inlet pipe is used to guide the liquid into the temporary storage tray, and one side of the temporary storage tray is connected to multiple output pipes.

[0009] As an optional solution of the automatic collection device for eDNA samples of aquatic organisms described in the present invention, the suction assembly includes a suction tube located between the storage tubes, the suction tube is fixedly connected to one end of the outer shell, a sealing disk is slidably connected to the inside of the suction tube, a square rod is fixedly connected to one side of the sealing disk, one end of the square rod is rotatably connected to the power output end of the hydraulic rod, a spiral column is fixedly connected to the other side of the sealing disk, the spiral column is slidably connected to a fixed column, and the fixed column is fixedly installed on the inner wall of the suction tube.

[0010] As an optional solution of the automatic eDNA sample collection device for aquatic organisms described in the present invention, the suction tube is connected to multiple pipes, the suction tube is connected to each storage tube through the pipe, and a one-way valve is installed in each pipe.

[0011] As an optional solution of the automatic eDNA sample collection device for aquatic organisms described in the present invention, the suction component also includes a first straw fixedly installed at one end of the shell, a first spring is installed in the first straw, one end of the first spring is in contact with a second straw, the second straw is located in the first straw, the second straw is slidably connected to the inner wall of the first straw, a first elastic sheet is fixedly installed on the inner wall of the first straw, the first elastic sheet is in contact with one end of the second straw, and the first straw is connected to the liquid inlet pipe.

[0012] As an optional solution of the automatic eDNA sample collection device for aquatic organisms described in the present invention, a second spring is installed in the second straw, one end of the second spring is in contact with a third straw, the third straw is slidably connected to the inner wall of the second straw, a second elastic sheet is fixedly installed on the inner wall of the second straw, the second elastic sheet is in contact with one end of the third straw, and one end of the third straw is used to contact with the top rod.

[0013] As an optional solution of the automatic eDNA sample collection device for aquatic organisms described in the present invention, the control component includes a control gear disc located at one end of the suction tube, the control gear disc is rotatably connected to the suction tube, the square rod passes through the middle of the control gear disc, the arc-shaped rack on the outside of the control gear disc is multi-section, and a plurality of sealing gear discs are provided on the outside of the control gear disc, the sealing gear disc is used to mesh with the arc-shaped rack, the sealing gear disc is rotatably connected to one end of the storage tube, and a plurality of elastic blocks are installed on the storage tube at one end of the sealing gear disc, and the elastic blocks are used to fix the position of the sealing gear disc.

[0014] As an optional solution of the automatic eDNA sample collection device for aquatic organisms described in the present invention, waterproof pads are provided on both sides of the sealing toothed disc to prevent liquid leakage, the sealing toothed disc is located between the output pipe and the liquid inlet hole, and a circular hole is opened on the sealing toothed disc, and the circular hole is used to connect the output pipe and the sealing toothed disc.

[0015] As an optional scheme for the collection method of the automatic collection device for eDNA samples of aquatic organisms described in the present invention, it includes the following steps:

[0016] S1: The user grasps the hand-held handle and then places the third straw into the first layer of water to be collected;

[0017] S2: The hydraulic lever is started to collect and store the liquid through the storage component through the cooperation of the internal suction component and the control component;

[0018] S3: With the operation of the hydraulic rod, in cooperation with the suction assembly, the second and third suction tubes are extended and retracted, so that the liquid sample in the lower layer can also be collected, and liquid samples in multiple layers can be collected;

[0019] S4: Take out the liquid in the storage tube, and then put the second straw and the third straw back in place for next use.

[0020] The present invention has the following beneficial effects:

[0021] 1. The automatic eDNA sample collection device for aquatic organisms can collect liquids of different depths in one use through the combined design of the first straw, the second straw and the third straw. At the same time, through the cooperation of multiple storage tubes, liquids of different depths can be stored separately, thereby improving the sampling efficiency.

[0022] 2. The automatic eDNA sample collection device for aquatic organisms realizes the opening and closing control of the liquid inlet hole of the storage tube through the combination of the control toothed disc and the sealing toothed disc and the multi-segment design of the arc-shaped rack, thereby controlling the flow of the liquid. At the same time, the design of the waterproof pad effectively prevents the occurrence of liquid leakage and ensures the accuracy of sampling. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0024] Figure 2 It is a schematic diagram of the internal structure of the present invention.

[0025] Figure 3 It is a schematic diagram of the cross-sectional structure of the temporary storage disk of the present invention.

[0026] Figure 4 It is a schematic diagram of the control component structure of the present invention.

[0027] Figure 5 It is a schematic diagram of the explosion structure of the storage tube and the sealing toothed disc of the present invention.

[0028] Figure 6 It is a schematic diagram of the overall cross-sectional structure of the present invention.

[0029] Figure 7 It is a schematic diagram of the overall cross-sectional structure of the present invention.

[0030] Figure 8 For the present invention Figure 6 Enlarged structural diagram at A in the middle.

[0031] Fig. 9 For the present invention Figure 6 Enlarged structural diagram at B in the middle.

[0032] Fig.10 This is a schematic diagram of the overall cross-sectional structure from another viewing angle of the present invention.

[0033] Fig.11 For the present invention Fig.10 Enlarged structural diagram at point C in the middle.

[0034] In the figure: 1. shell; 11. support shell; 2. support assembly; 21. fixing rod; 22. fixing disk; 23. hand-held handle; 24. hydraulic rod; 3. storage assembly; 31. storage tube; 32. liquid inlet hole; 33. temporary storage disk; 34. output tube; 35. liquid inlet tube; 36. pipeline; 37. one-way valve; 4. suction assembly; 41. suction tube; 42. sealing disk; 43. square rod; 44. spiral column; 45. first suction tube; 46. second suction tube; 47. third suction tube; 48. first spring; 49. second spring; 410. first elastic sheet; 411. second elastic sheet; 412. fixing column; 413. push rod; 5. control assembly; 51. control toothed disc; 52. blocking toothed disc; 53. round hole; 54. arc rack; 55. elastic block. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] For example, see Figure 1-Figure 11 The present invention discloses an automatic eDNA sample collection device for aquatic organisms, comprising a shell 1, one end of the shell 1 is fixedly connected to a support shell 11, one side of the support shell 11 is provided with a support assembly 2, the support assembly 2 comprises a plurality of fixed rods 21 fixedly mounted on the support shell 11, one end of the fixed rods 21 is fixedly connected to a fixed plate 22 for maintaining stability, one side of the fixed plate 22 is fixedly connected to a handheld grip 23 for user convenience, and the other side of the fixed plate 22 is provided with a hydraulic rod 24 for providing power;

[0037] A storage assembly 3 is provided inside the housing 1. The storage assembly 3 includes a plurality of storage tubes 31 for storing liquid. The storage tubes 31 are fixedly connected to the inside of one end of the housing 1. One end of the storage tubes 31 is provided with a liquid inlet 32 ​​for facilitating the inflow of liquid.

[0038] A suction assembly 4 is provided between the storage tubes 31 , and the suction assembly 4 is used to suck the liquid to be detected. A control assembly 5 is provided at one end of the storage tube 31 , and the control assembly 5 is used to control the flow of the liquid.

[0039] The existing automatic eDNA sample collection device for aquatic organisms often needs to use the device multiple times to collect liquids at different depths on the same water surface when sampling and collecting liquids. This method not only delays the collection time, but is also prone to errors during operation, affecting the accuracy of the sample and causing deviations in subsequent analysis data. The device has a suction component 4 that can collect and sample liquids at different depths during the same use process, and with the cooperation of the control component 5 and the storage component 3, liquids at different depths can be distinguished and stored separately, which is beneficial to the accuracy of subsequent analysis results.

[0040] The combination of multiple fixed rods 21 and fixed disks 22 provides stable support for the entire system, ensures the stability of the system during operation, prevents liquid leakage or equipment damage caused by shaking or tipping, and the design of the liquid inlet 32 ​​allows the water sample to flow into the storage tube 31 conveniently; the automatic suction of the liquid is realized through the cooperation of the hydraulic rod 24 and the suction component 4, which reduces the tediousness and errors of manual operation and improves work efficiency; the control component 5 can accurately control the flow of the liquid to ensure that the liquid flows at a predetermined speed and amount when needed, thereby improving the accuracy and controllability of the operation; the structural design forms a liquid processing system with complete functions, convenient operation, and precise controllability through the mutual cooperation of the support component 2, the storage component 3, the suction component 4, and the control component 5. The system not only improves the efficiency and accuracy of liquid processing, but also enhances the stability and safety of the system, which is of great significance for application scenarios that require precise control and detection of liquids.

[0041] Example 2: This example is an explanation based on Example 1. For details, please refer to Figure 1-Figure 11 The storage component 3 also includes a temporary storage tray 33 fixedly installed inside the shell 1. The temporary storage tray 33 is fixedly installed inside one end of the shell 1. The temporary storage tray 33 is used to provide a temporary storage space for the liquid. One side of the temporary storage tray 33 is connected to a liquid inlet pipe 35. The liquid inlet pipe 35 is used to guide the liquid into the temporary storage tray 33. One side of the temporary storage tray 33 is connected to multiple output pipes 34.

[0042] The suction assembly 4 includes a suction tube 41 located between the storage tubes 31, the suction tube 41 is fixedly connected to one end of the outer shell 1, a sealing disk 42 is slidably connected inside the suction tube 41, a square rod 43 is fixedly connected to one side of the sealing disk 42, one end of the square rod 43 is rotatably connected to the power output end of the hydraulic rod 24, a spiral column 44 is fixedly connected to the other side of the sealing disk 42, the spiral column 44 is slidably connected to a fixed column 412, and the fixed column 412 is fixedly installed on the inner wall of the suction tube 41.

[0043] The suction pipe 41 is connected to each storage pipe 31 through the pipeline 36 , and a one-way valve 37 is installed in each pipeline 36 .

[0044] The suction assembly 4 also includes a first suction pipe 45 fixedly installed at one end of the shell 1, a first spring 48 is installed in the first suction pipe 45, one end of the first spring 48 is in contact with the second suction pipe 46, the second suction pipe 46 is located in the first suction pipe 45, the second suction pipe 46 is slidably connected to the inner wall of the first suction pipe 45, a first elastic sheet 410 is fixedly installed on the inner wall of the first suction pipe 45, the first elastic sheet 410 is in contact with one end of the second suction pipe 46, and the first suction pipe 45 is connected to the liquid inlet pipe 35.

[0045] A second spring 49 is installed in the second straw 46, and one end of the second spring 49 is in contact with the third straw 47. The third straw 47 is slidably connected to the inner wall of the second straw 46. A second elastic sheet 411 is fixedly installed on the inner wall of the second straw 46, and the second elastic sheet 411 is in contact with one end of the third straw 47. One end of the third straw 47 is used to contact the top rod 413.

[0046] The control assembly 5 includes a control gear disc 51 located at one end of the suction tube 41, the control gear disc 51 is rotatably connected to the suction tube 41, the square rod 43 passes through the middle of the control gear disc 51, the arc-shaped rack 54 on the outside of the control gear disc 51 is multi-section, and a plurality of blocking gear discs 52 are provided on the outside of the control gear disc 51, the blocking gear disc 52 is used to mesh with the arc-shaped rack 54, the blocking gear disc 52 is rotatably connected to one end of the storage tube 31, and a plurality of elastic blocks 55 are installed at one end of the storage tube 31 located at the sealing gear disc 52, and the elastic blocks 55 are used to fix the position of the blocking gear disc 52.

[0047] Waterproof pads are provided on both sides of the blocking toothed disc 52 to prevent leakage. The blocking toothed disc 52 is located between the output pipe 34 and the liquid inlet 32 ​​. A circular hole 53 is provided on the blocking toothed disc 52 to connect the output pipe 34 with the blocking toothed disc 52 .

[0048] The following steps are involved:

[0049] S1: The user grasps the hand-held handle 23 and then places the third straw 47 on the first layer of water to be collected;

[0050] S2: Start the hydraulic rod 24 to collect and store the liquid through the storage component 3 through the cooperation of the internal suction component 4 and the control component 5;

[0051] S3: With the operation of the hydraulic rod 24, in cooperation with the suction assembly 4, the second suction tube 46 and the third suction tube 47 are extended and retracted, so that the liquid sample of the lower layer can also be collected, and multiple layers of liquid samples can be collected;

[0052] S4: Take out the liquid in the storage tube 31, and then put the second suction tube 46 and the third suction tube 47 back to their original positions for the next use.

[0053] By holding the hand-held handle 23 tightly, the third suction tube 47 is placed under the water surface where sampling is required, and then the hydraulic rod 24 is started. The hydraulic rod 24 resists the square rod 43, and the square rod 43 and the hydraulic rod 24 will not separate, so that the square rod 43 drives the sealing disk 42 to slide in the suction tube 41, and the sealing disk 42 slides in the first suction tube 41. When the sealing disk 42 slides, a negative pressure is generated on the side of the sealing disk 42 in the suction tube 41 close to the hydraulic rod 24, so that the storage tube 31 is sucked through the one-way valve 37 in the pipeline 36. At the same time, the storage tube 31 connected to the temporary storage tray 33 will suck the temporary storage tray 33. When the temporary storage tray 33 is sucked, the temporary storage tray 33 will suck the first suction tube 45, the second suction tube 46, and the third suction tube 47 through the liquid inlet tube 35, so that the liquid enters the temporary storage tray 33 through the liquid inlet tube 35. At the same time, the liquid in the temporary storage tray 33 will enter the storage tube 31 connected to the output tube 34 at this time. The combination of the temporary storage tray 33, the liquid inlet tube 35, and the output tube 34 ensures the smoothness and accuracy of liquid input. At the same time, the output tubes 34 correspond to the liquid inlet holes 32 respectively to ensure that each output tube 34 has a corresponding liquid inlet hole 32, and the extracted samples are input into different storage tubes 31 through different liquid inlet holes 32 to achieve accurate distribution of the liquid. The design of the temporary storage disk 33 can provide an appropriate temporary storage space for the liquid. When the circular hole 53 on the blocking toothed disk 52 does not connect the output tube 34 with the liquid inlet hole 32, the liquid sucked up at this time will be temporarily stored in the temporary storage disk 33. When the circular hole 53 connects the output tube 34 with the liquid inlet hole 32 , the liquid sample extracted from the temporary storage tray 33 will enter the storage tube 31 which is connected at this time. This design will not affect the suction of the liquid even if the liquid inlet hole 32 is not connected to the temporary storage tray 33; at the same time, a drain valve for draining liquid is provided on the suction tube 41. During the operation, a small amount of liquid sample may enter the suction tube 41. After use, the excess liquid can be discharged through the drain valve. At the same time, a liquid extraction valve is provided at one end of the storage tube 31, and the liquid sample collected in the storage tube 31 can be taken out through the liquid extraction valve.

[0054] The sealing disc 42 slides while driving the spiral column 44 to slide downward. The spiral column 44 slides while sliding with the fixed column 412. The fixed column 412 is designed so that the spiral groove on the spiral column 44 can rotate when sliding with the fixed column 412. The spiral column 44 rotates while driving the sealing disc 42 and the square rod 43 to rotate together. The control toothed disc 51 is rotated by the rotation of the square rod 43. The square rod 43 is designed so that the square rod 43 can drive the control toothed disc 51 to rotate together. The rotation of the control toothed disc 51 At the same time, the arc-shaped rack 54 is meshed with each blocking toothed disc 52, so that each blocking toothed disc 52 rotates together. The design of the arc-shaped rack 54 enables the control toothed disc 51 to be able to suck the liquid sample when it is not meshed with the arc-shaped rack 54. When the control toothed disc 51 is meshed with the arc-shaped rack 54, and when the circular hole 53 connects the output tube 34 with the liquid inlet hole 32, the liquid sample in the temporary storage disc 33 can be distributed to the corresponding storage tube 31. The liquid inlet hole 32 on the storage tube 31 where the sampling has been completed is blocked by the rotation of the blocking toothed disc 52, and at the same time, the next The rotation of the blocking toothed disc 52 connects the liquid inlet 32 ​​on the next storage tube 31 with the output tube 34 through the circular hole 53 on the blocking toothed disc 52. In this way, each storage tube 31 can collect samples in turn. At the same time, the waterproof pads on both sides of the blocking toothed disc 52 can prevent liquid from leaking between the output tube 34 and the liquid inlet 32. Through the design of the blocking toothed disc 52 and the circular hole 53, the connection between the output tube 34 of the temporary storage disc 33 and the liquid inlet 32 ​​of the storage tube 31 is controllable. When the blocking toothed disc 52 is rotated to a suitable position, the circular hole 53 The hole 53 will connect the liquid inlet hole 32 with the output pipe 34, so that the collected liquid can flow into the storage tube 31; through the design of the elastic block 55 located between the storage tube 31 and the blocking toothed disc 52, the blocking toothed disc 52 can be engaged with the elastic block 55 through the slot on the blocking toothed disc 52 after rotation. Through such a design, the position of the blocking toothed disc 52 can be limited, the friction between the blocking toothed disc 52 and the storage tube 31 can be increased, and the blocking toothed disc 52 can be prevented from rotating without reason, thereby providing a more stable channel for the liquid to enter the storage tube 31.

[0055] When the spiral column 44 slides downward, it drives the push rod 413 to slide downward together. A protrusion is provided at the first end of the push rod 413. Such a design prevents the push rod 413 from blocking the inside of the third suction tube 47, so that the liquid can flow in the third suction tube 47. When the protrusion of the push rod 413 slides to one end of the third suction tube 47 and contacts the third suction tube 47, the first storage tube 31 has collected enough liquid sample. As the push rod 413 continues to slide downward, the protrusion of the push rod 413 continuously contacts the third suction tube 47. When the contact force is greater than the maximum bearing range of the second elastic sheet 411, the third suction tube 47 will slide over the second elastic sheet 411. At the same time, under the action of the second spring 49, the third suction tube 47 can slide downward instantly, so that the third suction tube 47 can suck the liquid at a deep position.

[0056] At the same time, the elasticity of the second elastic piece 411 is smaller than that of the first elastic piece 410. Such a design enables the third straw 47 and the second straw 46 to slide downward in sequence, so that the third straw 47 and the second straw 46 will not slide over the second elastic piece 411 and the first elastic piece 410 at the same time. As the top rod 413 slides downward continuously, when the protruding block of the top rod 413 contacts the third straw 47 again, the second storage tube 31 has collected enough liquid sample. As the resistance force increases continuously, the third straw 47 drives the second straw 46 to slide downward together. When the resistance force is greater than the elasticity of the first elastic piece 410, the second straw 46 slides over the first elastic piece 410. At the same time, under the action of the first spring 48, the second straw 46 drives the third straw 47 to slide downward instantly, so that the third straw 47 can collect and sample deeper liquid.

[0057] Through the design of the first elastic sheet 410 and the second elastic sheet 411, when the raised block of the push rod 413 contacts the third straw 47, a certain amount of power can be accumulated for the third straw 47 and the second straw 46. When the force is greater than the limit of the first elastic sheet 410 or the second elastic sheet 411, the accumulated force enables the third straw 47 to instantly slide over the second elastic sheet 411, and the second straw 46 to instantly slide over the first elastic sheet 410. The accumulated force can also enable the second straw 46 and the third straw 47 to continue to slide downward. In addition, the cooperation of the first spring 48 and the second spring 49 enables the instantaneous sliding of the third straw 47. Such a design enables the liquid to be sucked from deeper inside as quickly as possible during the process of sucking the liquid sample, so that the sample is more standardized.

[0058] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the technical principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An automatic eDNA sample collection device for aquatic organisms, comprising a housing (1), characterized in that: One end of the housing (1) is fixedly connected to a support shell (11), one side of the support shell (11) is provided with a support assembly (2), the support assembly (2) comprises a plurality of fixing rods (21) fixedly mounted on the support shell (11), one end of the fixing rods (21) is fixedly connected to a fixing plate (22) for maintaining stability, one side of the fixing plate (22) is fixedly connected to a handheld handle (23) for convenient user control, and the other side of the fixing plate (22) is provided with a hydraulic rod (24) for providing power; A storage assembly (3) is arranged inside the housing (1), and the storage assembly (3) comprises a plurality of storage tubes (31) for storing liquid, the storage tubes (31) are fixedly connected to the inside of one end of the housing (1), and one end of the storage tubes (31) is provided with a liquid inlet hole (32) for facilitating the inflow of liquid; A suction component (4) is provided between the storage tubes (31), and the suction component (4) is used to suck the liquid to be tested. A control component (5) is provided at one end of the storage tube (31), and the control component (5) is used to control the flow of the liquid. The suction assembly (4) comprises a suction pipe (41) located between the storage pipes (31), the suction pipe (41) being fixedly connected to one end of the housing (1), a sealing disc (42) being slidably connected inside the suction pipe (41), a square rod (43) being fixedly connected to one side of the sealing disc (42), one end of the square rod (43) being rotatably connected to the power output end of the hydraulic rod (24), a spiral column (44) being fixedly connected to the other side of the sealing disc (42), the spiral column (44) being slidably connected to a fixed column (412), the fixed column (412) being fixedly mounted on the inner wall of the suction pipe (41), and a push rod (413) being provided at one end of the spiral column (44); The suction assembly (4) further comprises a first suction pipe (45) fixedly mounted on one end of the housing (1); a first spring (48) is mounted inside the first suction pipe (45); one end of the first spring (48) abuts against a second suction pipe (46); the second suction pipe (46) is located inside the first suction pipe (45); the second suction pipe (46) is slidably connected to the inner wall of the first suction pipe (45); a first elastic sheet (410) is fixedly mounted on the inner wall of the first suction pipe (45); the first elastic sheet (410) abuts against one end of the second suction pipe (46); and the first suction pipe (45) is connected to the liquid inlet pipe (35); A second spring (49) is installed in the second suction tube (46), one end of the second spring (49) is in contact with the third suction tube (47), the third suction tube (47) is slidably connected to the inner wall of the second suction tube (46), a second elastic sheet (411) is fixedly installed on the inner wall of the second suction tube (46), the second elastic sheet (411) is in contact with one end of the third suction tube (47), and one end of the third suction tube (47) is used to contact the top rod (413).

2. The automatic eDNA sample collection device for aquatic organisms according to claim 1, characterized in that: The storage assembly (3) further comprises a temporary storage tray (33) fixedly mounted inside the housing (1). The temporary storage tray (33) is fixedly mounted inside one end of the housing (1). The temporary storage tray (33) is used to provide a temporary storage space for liquid. One side of the temporary storage tray (33) is connected to a liquid inlet pipe (35). The liquid inlet pipe (35) is used to guide liquid into the temporary storage tray (33). One side of the temporary storage tray (33) is connected to a plurality of output pipes (34).

3. The automatic eDNA sample collection device for aquatic organisms according to claim 2, characterized in that: The suction pipe (41) is connected to a plurality of pipes (36); the suction pipe (41) is connected to each storage pipe (31) through the pipes (36); and each pipe (36) is provided with a one-way valve (37).

4. The automatic eDNA sample collection device for aquatic organisms according to claim 3, characterized in that: The control assembly (5) comprises a control toothed disc (51) located at one end of the suction tube (41), the control toothed disc (51) being rotatably connected to the suction tube (41), the square rod (43) passing through the middle of the control toothed disc (51), the arc-shaped rack (54) on the outside of the control toothed disc (51) being multi-segmented, a plurality of blocking toothed discs (52) being provided on the outside of the control toothed disc (51), the blocking toothed disc (52) being used for meshing with the arc-shaped rack (54), the blocking toothed disc (52) being rotatably connected to one end of the storage tube (31), a plurality of elastic blocks (55) being installed on one end of the storage tube (31) located at the blocking toothed disc (52), the elastic blocks (55) being used for fixing the position of the blocking toothed disc (52).

5. The automatic eDNA sample collection device for aquatic organisms according to claim 4, characterized in that: Waterproof pads for preventing liquid leakage are provided on both sides of the blocking toothed disc (52); the blocking toothed disc (52) is located between the output pipe (34) and the liquid inlet hole (32); a circular hole (53) is provided on the blocking toothed disc (52); the circular hole (53) is used to connect the output pipe (34) and the blocking toothed disc (52).

6. The method for collecting eDNA samples of aquatic organisms according to any one of claims 1 to 5, characterized in that: The following steps are involved: S1: The user grasps the hand-held handle (23) and then places the third straw (47) onto the surface of the first layer of water to be collected; S2: starting the hydraulic lever (24) to collect and store the liquid through the storage component (3) through the cooperation of the internal suction component (4) and the control component (5); S3: With the operation of the hydraulic rod (24), in cooperation with the suction assembly (4), the second suction tube (46) and the third suction tube (47) are extended and retracted, so that the liquid sample in the lower layer can also be collected, and multiple layers of liquid samples can be collected; S4: taking out the liquid in the storage tube (31), and then returning the second straw (46) and the third straw (47) to their original positions for the next use.

Images