A culture device for sterile seedlings of alfalfa

Abstract

The application provides a culture device for alfalfa seed aseptic seedlings, and relates to the field of alfalfa seed culture, which comprises a placing rack, a light shielding component, a temperature control device and a plurality of light supplement lamps; the placing rack comprises a mounting frame, a top plate and a plurality of bearing plates, the top plate is installed on the top of the mounting frame, and the plurality of bearing plates are installed at intervals in the interior of the mounting frame; the light shielding component is used for shielding the mounting frame and preventing natural light from entering the interior of the mounting frame. The culture device for alfalfa seed aseptic seedlings can be used for placing culture dishes on the bearing plates, shielding natural light through the light shielding component, and cooperating with the light supplement lamps and the temperature control device and other components to respectively realize dark culture, light and dark alternating culture and light culture of alfalfa seeds, so that the growth conditions of the alfalfa seeds cultured under different light regulation can be compared, and the light condition most suitable for the growth of the alfalfa seeds is determined.

Description

Technical Field

[0001] This invention relates to the field of alfalfa seed cultivation, and more particularly to a device for cultivating sterile alfalfa seedlings. Background Technology

[0002] Yellow alfalfa is a widely distributed wild leguminous forage grass. Compared to alfalfa, yellow alfalfa is more cold-resistant, disease- and pest-resistant, and has stronger stress resistance. However, as a wild species, yellow alfalfa also has some inherent drawbacks, such as inconsistent flowering periods, leading to inconsistent seed maturity, low yield, high hard seed rate, and low germination rate. These factors all affect the large-scale cultivation of yellow alfalfa. Tissue culture technology involves placing isolated organs, tissues, or cells of an organism in a culture medium under sterile conditions and placing them in a suitable environment for continuous culture to obtain cells, tissues, or individuals. The culture of sterile seedlings is a crucial step in tissue culture. Therefore, research on the culture technology of sterile yellow alfalfa seedlings can, on the one hand, rapidly obtain a large number of high-quality sterile seedlings, and on the other hand, provide materials for subsequent breeding research, genetic engineering, and other aspects of yellow alfalfa, providing a technical foundation for the genetic transformation and utilization of yellow alfalfa.

[0003] The cultivation of sterile seedlings is a crucial step in the verification of transgenic systems and functional genes. The culture material is usually derived from seeds, so seed treatment, culture medium selection, and culture conditions are particularly important. Among these, light conditions are a key factor affecting the germination rate of alfalfa seeds. Alfalfa seeds need to be cultivated under different light conditions to determine the optimal light conditions. In existing technologies, equipment used for seed cultivation, such as cultivation racks, usually have the function of supplemental lighting, but lack the function of autonomously changing light conditions, such as blocking natural light to achieve dark cultivation, light cultivation, and light-dark alternation cultivation. Dark cultivation relies on blocking the light-transmitting parts of the cultivation room, which is relatively limited in use. Moreover, when some plants in a cultivation room need natural light and others need dark cultivation, the cultivation room cannot meet both needs.

[0004] Therefore, it is necessary to provide a sterile seedling cultivation device for alfalfa seeds to solve the above-mentioned technical problems. Summary of the Invention

[0005] This invention provides a sterile seedling cultivation device for alfalfa seeds, which solves the problem that existing cultivation devices lack the function of autonomously changing light conditions.

[0006] To solve the above-mentioned technical problems, the present invention provides a cultivation device for sterile seedlings of alfalfa seeds, comprising: a placement rack, a light-shielding component, a temperature control device, a lifting cylinder, and multiple supplementary lights, multiple connecting mechanisms, and multiple fixing components; The placement rack includes a mounting frame, a top plate, and multiple support plates. The top plate is slidably mounted on the top of the mounting frame, and the multiple support plates are spaced apart and installed inside the mounting frame. Multiple supplementary lights are correspondingly installed at the bottom of the top plate and the bottom of the support plate; The light-shielding component is used to block the mounting frame and prevent natural light from entering the interior of the mounting frame; The temperature control device is mounted on the mounting frame and is used to regulate the temperature inside the mounting frame. The lifting cylinder is installed on the top of the mounting frame, and the output end of the lifting cylinder is connected to the top plate. Each of the bearing plates is detachably connected to the mounting frame through a fastener. The connecting mechanism includes a connecting arm and an assembly head. One end of the multiple connecting arms is installed at the bottom of the top plate and the bottom of the bearing plate, and the multiple assembly heads are installed at the other end of the corresponding connecting arm. The bearing plate is provided with a first assembly hole that is adapted to the assembly head.

[0007] Preferably, the temperature control device includes a mounting plate and an exhaust fan, the mounting plate being mounted on the mounting frame and the exhaust fan being mounted on the mounting plate.

[0008] Preferably, the alfalfa seed sterile seedling cultivation device further includes multiple reflective tin foils, which are correspondingly installed on the top of each of the support plates.

[0009] Preferably, the top of the support plate has a placement groove, and the reflective tin foil is placed inside the placement groove.

[0010] Preferably, the alfalfa seed sterile seedling cultivation device further includes a lifting cylinder, multiple connecting mechanisms, and multiple fixing components. The lifting cylinder is installed on the top of the mounting frame, and the output end of the lifting cylinder is connected to the top plate. The top plate is slidably connected to the mounting frame. Each bearing plate is detachably connected to the mounting frame via a fixing component. The connecting mechanism includes a connecting arm and an assembly head. One end of the multiple connecting arms is installed at the bottom of the top plate and the bottom of the bearing plate, respectively. The multiple assembly heads are installed at the other end of the corresponding connecting arm. The bearing plate has a first assembly hole adapted to the assembly head.

[0011] Preferably, the fixing component includes a square tube, a support block, a connecting shaft, and an end cap. The square tube is mounted on the bearing plate. One end of the support block is slidably disposed inside the square tube. One end of the connecting shaft passes through the square tube and is connected to the support block. The end cap is mounted on the other end of the connecting shaft. The mounting frame has multiple second mounting holes. The other end of the support block is inserted into a corresponding second mounting hole. The top surface of the support block is set as an inclined surface.

[0012] Preferably, the assembly head includes a movable cap, a support cap, and multiple driving blocks. The multiple driving blocks are arranged around the connecting arm and are rotatably connected to the connecting arm. The support cap is installed at the bottom end of the connecting arm, and the movable cap is sleeved on the connecting arm and located between the support cap and the driving blocks.

[0013] Preferably, the mounting frame further includes a slide rod, the bottom end of which is mounted on the mounting frame, and the top end of which passes through a plurality of the bearing plates and the top plate in sequence.

[0014] Compared with related technologies, the aseptic seedling cultivation device for alfalfa seeds provided by the present invention has the following beneficial effects: This invention provides a cultivation device for sterile seedlings of alfalfa. In use, the culture dish is placed on a support plate, and natural light is blocked by a light-blocking component. With the help of supplemental lighting and a temperature control device, alfalfa seeds can be cultured in the dark, in alternating light and dark, and in light. This allows for comparison of the growth of alfalfa seeds under different light conditions, and determination of which light condition is most suitable for the growth of alfalfa seeds. Attached Figure Description

[0015] Figure 1 A schematic diagram of the front structure of the alfalfa seed sterile seedling cultivation device provided by the present invention; Figure 2 Comparison of the aseptic seedlings of alfalfa provided by the present invention after 7 days of growth under different light conditions; Figure 3 Comparison of the aseptic seedlings of alfalfa provided by the present invention after 11 days of growth under different light conditions; Figure 4 A flowchart illustrating the steps of the method for cultivating aseptic seedlings of alfalfa seeds provided by the present invention; Figure 5 A schematic diagram of the rear structure of the cultivation device for sterile seedlings of alfalfa provided by the present invention; Figure 6 for Figure 1 A front view of the apparatus for cultivating sterile seedlings of alfalfa seeds; Figure 7 for Figure 1 A partial cross-sectional view of the light-shielding component shown; Figure 8 for Figure 4 The enlarged schematic diagram of part A shown below; Figure 9 for Figure 6 The enlarged schematic diagram of section B is shown below; Figure 10 This is a schematic diagram illustrating the principle of lowering the uppermost supporting plate provided by the present invention, wherein, Figure 10 (a) is a schematic diagram of the initial state of the uppermost supporting plate. Figure 10 (b) is a schematic diagram of the lifting cylinder pushing down the top plate to insert the assembly head into the assembly hole. Figure 10 (c) is a schematic diagram showing the lifting cylinder continuing to push the top plate down so that the assembly head passes through the assembly hole. Figure 10 (d) is a schematic diagram showing how the uppermost bearing plate lifts the fixing component, causing the support block to separate from the second assembly hole; Figure 11 for Figure 10 The enlarged schematic diagram shown below, in which, Figure 11 (a) is Figure 10 (a) is an enlarged schematic diagram. Figure 11 (b) is Figure 10 (b) is an enlarged schematic diagram. Figure 11 (c) is Figure 10 The enlarged schematic diagram in (c) Figure 11 (d) is Figure 10 (d) Enlarged schematic diagram; Figure 12 This is a schematic diagram of the lowering placement frame provided by the present invention located behind the two upper support plates.

[0016] Numbering on the map: 1. Placement rack; 11. Mounting frame; 12. Top plate; 13. Support plate; 14. Slide rod; 111. Second assembly hole; 131. First assembly hole; 2. Fill light; 3. Reflective aluminum foil; 4. Light-blocking components; 41. Storage cylinder; 42. Motor; 43. Rotating shaft; 44. Light-blocking curtain; 411. Opening; 5. Temperature control device; 51. Mounting plate; 52. Exhaust fan; 511. Second positioning component; 6. Fastener; 61. Square tube; 62. Support block; 63. Connecting shaft; 64. End cap; 7. Connecting mechanism; 71. Connecting arm; 72. Assembly head; 721. Driving block; 722. Movable cap; 723. Support cap; 8. Lifting cylinder; 9. Sunshade; 91. First positioning element; 92. Through hole; 10. Petri dish. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0018] This invention provides a method for cultivating sterile seedlings from yellow alfalfa seeds.

[0019] Please refer to the following: Figure 4 In one embodiment of the present invention, a method for cultivating aseptic seedlings of alfalfa seeds includes: S1. Seed peeling treatment: Select plump seeds, break the hard shell with the peeling part, rinse the seeds under running water for no less than 10 minutes after peeling, and then let them air dry. S2. Disinfection treatment: Place the seeds obtained in step S1 in 70% alcohol for 30-40 seconds, then rinse with sterile water 3-5 times; then disinfect with 0.1% mercuric chloride for 6-14 minutes, and finally rinse with sterile water 3-5 times. S3. Culture medium selection: The sterilized seeds were cultured in 1 / 2 MS medium with a sucrose content of 2%-3% and an agarose content of 0.65%-0.75%. S4. Set culture conditions: Place the 1 / 2MS culture medium on a culture device equipped with supplemental lighting in an indoor environment where natural light is visible and the indoor culture temperature is 23℃-27℃.

[0020] The seeds were polished using a grinding component to soften the hardened surface, making it easier for them to break through the outer skin and germinate. They were then disinfected with 70% alcohol for 30-40 seconds and rinsed 3-5 times with sterile water. After disinfection with 0.1% mercuric chloride for 12-14 minutes and rinsing with sterile water, the disinfected seeds were placed in 1 / 2 MS medium. The indoor culture temperature was set at 25℃, and the seeds were kept under visible natural light with supplemental lighting. The germination results were good.

[0021] In S2, the interval between alcohol disinfection and mercuric chloride disinfection shall not be less than 2 minutes.

[0022] Please see Figure 2 and Figure 3Furthermore, the present invention also places the culture medium under different light conditions for cultivation, wherein from left to right, dark culture, light culture, and light-dark alternating culture are used (where light culture and dark culture are alternated every 12 hours). As can be seen from the figure, the growth is the best under light culture. The light culture is carried out under supplemental lighting conditions, including supplemental lighting lamps and visible natural light.

[0023] The abrasive component can be sandpaper or a grinding wheel, etc.

[0024] Preferably, the disinfection process in S2 is completed on a clean bench.

[0025] By performing disinfection on a clean bench, a sterile working environment is improved, ensuring the production of sterile seeds.

[0026] The present invention also provides a device for cultivating sterile seedlings of alfalfa.

[0027] Please see Figure 1 The cultivation device for sterile alfalfa seedlings is used for the cultivation method of sterile alfalfa seedlings. The cultivation device for sterile alfalfa seedlings includes: a placement rack 1, a light-shielding component 4, a temperature control device 5, and multiple supplementary lights 2. The placement rack 1 includes a mounting frame 11, a top plate 12, and a plurality of support plates 13. The top plate 12 is installed on the top of the mounting frame 11, and the plurality of support plates 13 are installed at intervals inside the mounting frame 11. Multiple supplementary lights 2 are respectively installed at the bottom of the top plate 12 and the bottom of the support plate 13; The light-shielding component 4 is used to shield the mounting frame 11 and prevent natural light from entering the interior of the mounting frame 11; The temperature control device 5 is installed on the mounting frame 11, and the temperature control device 5 is used to regulate the temperature inside the mounting frame 11.

[0028] In use, the petri dish 10 is placed on the support plate 13, and the natural light is blocked by the light-blocking component 4. With the help of the supplementary light lamp 2 and the temperature control device 5, the alfalfa seeds can be cultured in the dark, alternating light and dark, and light. This allows for comparison of the growth of alfalfa seeds under different light conditions, and determination of which light condition is most suitable for the growth of alfalfa seeds.

[0029] As an optional embodiment, the light-blocking component 4 is a curtain or the like, used to block the light-transmitting components such as the windows of the culture room to block natural light.

[0030] As another alternative to this embodiment, the light-blocking component 4 is used to block the mounting frame 11. In conjunction with the curtains of the culture room, the light-blocking effect can be further improved. When the blocking effect of the curtains of the culture room is not good, the light-blocking effect can be further guaranteed.

[0031] Please see Figure 5 and Figure 7 In this embodiment, the light-shielding component 4 includes a storage cylinder 41, a motor 42, a rotating shaft 43, and a light-shielding curtain 44. The storage cylinder 41 is installed on the top of the mounting frame 11 and partially suspended outside the mounting frame 11. An opening 411 is opened at the bottom of the storage cylinder 41. The motor 42 is installed at one end of the storage cylinder 41. The rotating shaft 43 is rotatably installed inside the storage cylinder 41 and connected to the output end of the motor 42. The light-shielding curtain 44 is wound around the rotating shaft 43, with one end connected to the rotating shaft 43 and the other end extending out of the storage cylinder 41 through the opening 411. Guide rollers are provided inside the storage cylinder 41 and on both sides of the opening 411 to guide the light-shielding curtain 44. A weight-adding block is provided at the bottom of the light-shielding curtain 44 to assist the light-shielding curtain 44 in descending.

[0032] When it is necessary to shade the mounting frame 11, the motor 42 drives the rotating shaft 43 to rotate counterclockwise, releasing the shading curtain 44. The shading curtain 44 extends downward to block the front side of the mounting frame 11. Subsequently, the motor 42 drives the rotating shaft 43 to rotate in the opposite direction, and the rotating shaft 43 retracts the shading curtain 44.

[0033] Meanwhile, light shields 9 are set at both ends of the mounting frame 11 to help block the light at both ends of the mounting frame 11. The back of the mounting frame 11 can be set against a wall; similarly, light shielding components 4 can be set on the back of the mounting frame 11 that is not set against a wall to block the light.

[0034] Both the blackout cover 9 and the blackout curtain 44 are preferably made of breathable fabric.

[0035] Please see Figure 1 and Figure 5 In this embodiment, the temperature control device 5 includes a mounting plate 51 and an exhaust fan 52. The mounting plate 51 is mounted on the mounting frame 11, and the exhaust fan 52 is mounted on the mounting plate 51.

[0036] By setting up an exhaust fan 52, the airflow inside the mounting frame 11 can be increased, keeping the temperature between each culture dish 10 inside the mounting frame 11 the same as the temperature of the external environment (i.e., the culture chamber) of the mounting frame 11.

[0037] In particular, when the light-shielding component 4 is used to block the mounting frame 11, resulting in isolation between the interior and exterior environments of the mounting frame 11, the exhaust fan 52 operates to circulate the air inside and outside the mounting frame 11, thereby regulating the temperature environment inside the mounting frame 11.

[0038] Multiple temperature control devices 5 are preferably provided, corresponding to the number of support plates 13, and preferably installed at one end of the mounting frame 11, located above the corresponding support plate 13.

[0039] When light shields 9 are installed at both ends of the mounting frame 11, multiple light shields 9 are installed at the end where the temperature control device 5 is installed to block the light-transmitting part between the upper part of the mounting plate 51 and the corresponding support plate 13 or between the upper part of the mounting plate 51 and the top plate 12. The light shield 9 at the other end of the mounting frame 11 completely blocks the other end of the mounting frame 11. The light shield 9 has through holes 92 at the position above each support plate 13 to facilitate the flow of air inside and outside the mounting frame 11.

[0040] The tops of multiple light shields 9 located at one end of the temperature control device 5 are connected to the top plate 12 and the mounting plate 51, respectively. A first positioning element 91 is provided on the light shield 9 connected to the mounting plate 51, and a corresponding second positioning element 511 is provided on the mounting plate 51. Thus, when not blocking light, the light shield 9 can be folded upwards and positioned by the assembly of the first positioning element 91 and the second positioning element 511. The light shield 9 connected to the top plate 12 can be directly placed upwards on the top plate 12.

[0041] The first positioning element 91 and the second positioning element 511 can be magnetic blocks with different polarities, respectively installed on the light shield 9 and the mounting plate 51; or they can be male and female buckles, respectively installed on the light shield 9 and the mounting plate 51.

[0042] The top of the sunshade 9 on the other side of the mounting frame 11 is connected to the top of the mounting frame 11. Without the need for a device, it can be directly retracted onto the top plate 12.

[0043] In other embodiments, the temperature control device 5 can also be an air conditioning unit, a temperature sensor, a main pipe, and multiple branch pipes. The temperature sensor is installed inside the mounting frame 11. The air inlet end of the main pipe is connected to the air conditioning unit. The air inlet ends of the multiple branch pipes are connected to the multiple outlet ends of the main pipe. The air outlet ends of the multiple branch pipes are connected to the mounting frame 11 and suspended above the corresponding support plate 13. The temperature inside the mounting frame 11 is detected by the temperature sensor, and the start and stop of the air conditioning unit are controlled.

[0044] Please see Figure 1 and Figure 5 As a preferred embodiment, the alfalfa seed sterile seedling cultivation device further includes a plurality of reflective tin foils 3, which are respectively installed on the top of each of the carrier plates 13.

[0045] By setting reflective tin foil 3, the light emitted by the supplementary light lamp 2 can be reflected, increasing the light intensity and uniformity, providing more sufficient light for the seedlings, which helps photosynthesis and promotes the growth and development of the seedlings.

[0046] Preferably, the top of the support plate 13 is provided with a placement groove, and the reflective tin foil 3 is disposed inside the placement groove.

[0047] By setting up a placement slot, the reflective aluminum foil 3 can be positioned to prevent displacement.

[0048] When the height of the mounting frame 11 is set high, the number of support plates 13 is set to be large, such as more than four. It is not easy for staff to place culture equipment such as culture dishes 10 on the support plates 13 located on the upper layer. At the same time, it is not easy to observe the growth of seedlings in the culture dishes 10 placed on the upper layer, and it is not easy to clean the support plates 13 located on the upper layer later.

[0049] To resolve the above issues, please refer to [link / reference]. Figure 1 and Figure 5 The alfalfa seed sterile seedling cultivation device further includes a lifting cylinder 8, multiple connecting mechanisms 7, and multiple fixing parts 6. The lifting cylinder 8 is installed on the top of the mounting frame 11, and the output end of the lifting cylinder 8 is connected to the top plate 12. The top plate 12 is slidably connected to the mounting frame 11. Each of the bearing plates 13 is detachably connected to the mounting frame 11 through the fixing parts 6. The connecting mechanism 7 includes a connecting arm 71 and an assembly head 72. One end of the multiple connecting arms 71 is installed at the bottom of the top plate 12 and the bottom of the bearing plate 13, respectively. The multiple assembly heads 72 are installed at the other end of the corresponding connecting arm 71. The bearing plate 13 has a first assembly hole 131 adapted to the assembly head 72.

[0050] When it is necessary to place culture equipment such as petri dishes 10 onto the upper support plate 13, or to observe the growth of seedlings placed in the upper petri dishes 10, or to clean the upper support plate 13.

[0051] At this time, the lifting cylinder 8 pushes down the top plate 12, and the top plate 12 slides down along the mounting frame 11. The assembly head 72 in the connecting mechanism 7 assembles with the first assembly hole 131 on the uppermost bearing plate 13. Then, the corresponding fastener 6 is unlocked, so that the uppermost bearing plate 13 is unlocked from the mounting frame 11. The connecting mechanism 7 supports the bearing plate 13. At this time, the distance between the bearing plate 13 and the top plate 12 is the length of the connecting arm 71. This distance is greater than the height of the culture equipment such as the culture dish 10. There is also a gap between the culture dish 10 and the supplemental light 2 to prevent the top plate 12 from pressing on the seedlings. Similarly, the support plate 13 located at the next upper level can be lowered, such as... Figure 12 This allows the height of the upper support plate 13 to be reduced without affecting the culture equipment such as the culture dish 10, making it easier to place and observe the culture dish 10 and to facilitate subsequent cleaning.

[0052] Preferably, there are two lifting cylinders 8, which are installed at both ends of the mounting frame 11, and the output end of the lifting cylinder 8 is connected to the two ends of the top plate 12 respectively; the lifting cylinder 8 is a pneumatic cylinder, a hydraulic cylinder or an electric push cylinder.

[0053] In a preferred embodiment, a connecting mechanism 7 is provided at both ends of the top plate 12 and the support plate 13. Depending on the application, the connecting mechanism 7 can be provided on one or more of the upper support plates 13. In this embodiment, the connecting mechanisms 7 are provided on the three upper support plates 13. The positions of the connecting mechanisms 7 on adjacent support plates 13 are staggered, and the corresponding first mounting holes 131 are staggered, thereby enabling the connecting mechanism 7 to be assembled with the corresponding first mounting hole 131.

[0054] As a preferred embodiment of this example, two fasteners 6 are installed at both ends of each bearing plate 13, and the two support arms at both ends of the mounting frame 11 are detachably installed.

[0055] Please see Figure 9 As an optional embodiment of this example, the fixing member 6 includes a square tube 61, a support block 62, a connecting shaft 63, and an end cap 64. The square tube 61 is mounted on the bearing plate 13. One end of the support block 62 is slidably disposed inside the square tube 61. One end of the connecting shaft 63 passes through the square tube 61 and is connected to the support block 62. The end cap 64 is mounted on the other end of the connecting shaft 63. The mounting frame 11 has a plurality of second mounting holes 111. The other end of the support block 62 is inserted into a corresponding second mounting hole 111. The top surface of the support block 62 is set as an inclined surface.

[0056] When the lifting cylinder 8 pushes down the top plate 12, assembling the assembly head 72 in the connecting mechanism 7 with the first assembly hole 131, the bearing plate 13 is first lifted. The bearing plate 13 drives the fixing member 6 to move upward. The inclined surface at the top of the support block 62 acts on the upper side wall of the second assembly hole 111, and the inclined surface at the top of the support block 62 is squeezed. The support block 62 moves towards the inside of the square cylinder 61 and moves out of the second assembly hole 111. Thus, the bearing plate 13 and the mounting frame 11 can be unlocked automatically without the need for the operator to manually adjust the fixing member 6 to unlock the bearing plate 13 and the mounting frame 11, thus achieving the function of quick unlocking.

[0057] The unlocking principle is the same for the other carrier plates 13.

[0058] When the subsequent fastener 6 is reassembled with the second assembly hole 111 on the mounting frame 11, the lifting cylinder 8 drives the bearing plate 13 to rise through the top plate 12 and the connecting mechanism 7, so that the support block 62 in the fastener 6 is aligned with the second assembly hole 111. The worker pushes the end cap 64, and the end cap 64 pushes the support block 62 through the connecting shaft 63, so that the support block 62 is inserted into the second assembly hole 111 again, thereby realizing the detachable assembly of the bearing plate 13 and the mounting frame 11.

[0059] As an alternative embodiment, the fastener 6 includes an assembly plate and a locating pin. The assembly plate is mounted on the support plate 13, and the locating pin passes through the assembly plate and is threadedly connected to it. Assembly is achieved by tightening the locating pin, which is then inserted into the second assembly hole 111. A nut or a threaded hole is provided on the assembly plate to achieve the threaded connection with the locating pin.

[0060] Please see Figure 8 In this embodiment, the assembly head 72 includes a movable cap 722, a support cap 723, and a plurality of driving blocks 721. The plurality of driving blocks 721 are arranged around the connecting arm 71 and are rotatably connected to the connecting arm 71. The support cap 723 is installed at the bottom end of the connecting arm 71. The movable cap 722 is sleeved on the connecting arm 71 and is located between the support cap 723 and the driving blocks 721.

[0061] In this embodiment, when the drive block 721 is in a naturally flattened state, such as Figure 8 As shown, the end of the drive block 721 away from the connecting arm 71 extends outside the first mounting hole 131.

[0062] Please see Figure 11 In (b), when the assembly head 72 is assembled with the first assembly hole 131, the lifting cylinder 8 lowers the top plate 12. The top plate 12 drives the assembly head 72 to follow and lower via the connecting arm 71. The assembly head 72 passes through the first assembly hole 131. During the passage, multiple driving blocks 721 act against the wall of the first assembly hole 131, causing the driving blocks 721 to rotate upward relative to the connecting arm 71 and retract. After the assembly head 72 has completely passed through the first assembly hole 131, each driving block 721 rotates downward under its own weight and is positioned on the movable cap 722, as shown. Figure 11 As shown in (c), at this time, the lifting cylinder 8 lifts the top plate 12, and the top plate 12 drives the connecting mechanism 7 to move upward. At this time, the driving block 721 acts on the bottom of the bearing plate 13, as shown in (c). Figure 11 As shown in (d), due to the function of the movable cap 722 and the support cap 723, the driving block 721 can be limited, preventing the driving block 721 from rotating downward. Thus, the driving block 721 can support the bearing plate 13, realizing the automatic assembly of the assembly head 72 and the first assembly hole 131 without manual assembly.

[0063] The principle of the connecting mechanism 7 on the support plate 13 corresponding to the first mounting hole 131 on other support plates 13 is the same as above.

[0064] By setting the movable cap 722, when the assembly head 72 is subsequently separated from the first assembly hole 131, after the corresponding fixing part 6 is assembled with the mounting frame 11, and the assembly head 72 is in the position as shown... Figure 11 When in state (c), the movable cap 722 can be lifted, and the movable cap 722 pushes up multiple driving blocks 721, causing the multiple driving blocks 721 to retract, so that the diameter of the assembly head 72 is smaller than the diameter of the first assembly hole 131, thereby allowing it to pass through the first assembly hole 131 and achieve unlocking.

[0065] The inner wall of the movable cap 722 is fitted with a rubber ring. After the multiple driving blocks 721 are lifted, the rubber ring is squeezed, which allows the movable cap 722 to maintain its current state and limit the multiple driving blocks 721. At this time, the assembly head 72 in the connecting mechanism 7 at the other end of the mounting frame 11 can be adjusted in the same way. After the assembly head 72 is separated from the first assembly hole 131, each movable cap 722 is moved downward to release the limitation on the driving block 721.

[0066] The number of driving blocks 721 is not less than two, and in this embodiment there are four. One end of the driving block 721 is connected to the connecting arm 71 through a rotating shaft. The two ends of the rotating shaft are connected to the connecting arm 71 through fixed ears. The driving block 721 is sleeved on the rotating shaft to form a rotating connection.

[0067] As an optional approach in this embodiment, a torsion spring can be fitted onto the rotating shaft. One end of the torsion spring is connected to the driving block 721, and the other end is connected to the connecting arm 71. The torsion spring is used to keep the driving block 721 in a horizontally extended state.

[0068] The elastic force of the torsion spring is much less than the weight of the bearing plate 13.

[0069] In other embodiments, the assembly head 72 includes a mounting tube, a spring, and two assembly blocks. The mounting tube is horizontally mounted on the connecting arm 71. The two assembly blocks are keyed to both ends of the mounting tube, and the spring is connected to one end of the two assembly blocks. The bottom of the assembly blocks is set as an inclined surface.

[0070] When the assembly head 72 is assembled with the first assembly hole 131, the inclined surfaces at the bottom of the two assembly blocks are squeezed by the wall of the first assembly hole 131, causing the springs of the two assembly blocks to enter the mounting tube. When the assembly head 72 has completely passed through the first assembly hole 131, the springs push the two assembly blocks to pop out, thus realizing the assembly of the connecting mechanism 7 with the support plate 13.

[0071] In a preferred embodiment, the mounting frame 11 further includes a slide rod 14, the bottom end of which is mounted on the mounting frame 11, and the top end of which passes through a plurality of the bearing plates 13 and the top plate 12 in sequence.

[0072] By setting the slide bar 14, the top plate 12 and the bearing plate 13 are limited in the horizontal direction, thereby improving the overall stability of the placement frame 1.

[0073] The working principle of the alfalfa seed sterile seedling cultivation device provided by the present invention is as follows: In use, the petri dish 10 is placed on the support plate 13. By blocking natural light through the light-blocking component 4, the alfalfa seeds can be cultured in the dark, in alternating light and dark, and in light. This allows for comparison of the growth of alfalfa seeds under different light conditions, and determination of which light is most suitable for the growth of alfalfa seeds.

[0074] In use, when it is necessary to place culture equipment such as culture dish 10 onto the upper support plate 13, or to observe the growth of seedlings placed in the upper culture dish 10, or to clean the upper support plate 13.

[0075] The lifting cylinder 8 pushes down the top plate 12, and the top plate 12 slides down along the mounting frame 11. The assembly head 72 in the connecting mechanism 7 assembles with the first assembly hole 131 on the uppermost bearing plate 13.

[0076] Specifically, when the assembly head 72 is assembled with the first assembly hole 131, the lifting cylinder 8 lowers the top plate 12. The top plate 12 drives the assembly head 72 to follow the descent via the connecting arm 71. The assembly head 72 passes through the first assembly hole 131. During this process, multiple driving blocks 721 act against the wall of the first assembly hole 131, causing the driving blocks 721 to rotate upwards relative to the connecting arm 71 and retract. After the assembly head 72 has completely passed through the first assembly hole 131, each driving block 721 rotates downwards under its own weight and rests on the movable cap 722. Figure 11 As shown in (c), at this time, the lifting cylinder 8 lifts the top plate 12, and the top plate 12 drives the connecting mechanism 7 to move upward. At this time, the driving block 721 acts on the bottom of the bearing plate 13, as shown in (c). Figure 11 As shown in (d), due to the function of the movable cap 722 and the support cap 723, the driving block 721 can be limited, so that the driving block 721 cannot rotate downward. Thus, the driving block 721 can support the bearing plate 13, realizing the automatic assembly of the assembly head 72 and the first assembly hole 131. At this point, the support plate 13 can be raised further. The support plate 13 drives the fixing member 6 to move upward. The inclined surface at the top of the support block 62 interacts with the upper side wall of the second assembly hole 111. The inclined surface at the top of the support block 62 is squeezed, and the support block 62 moves towards the inside of the square tube 61 and moves out of the second assembly hole 111. Thus, the unlocking between the support plate 13 and the mounting frame 11 can be achieved automatically without the need for the staff to manually adjust the fixing member 6 to unlock the support plate 13 and the mounting frame 11, thus achieving the function of quick unlocking.

[0077] Similarly, the support plate 13 located at the next upper level can be lowered, such as... Figure 12 Similarly, the lower support plate 13 can be lowered, thereby reducing the height of the upper support plate 13 without affecting the culture equipment such as the culture dish 10, making it easier to place and observe the culture dish 10 and to clean the support plate 13 later.

[0078] The above description is merely an embodiment of the present invention and does not limit the patent scope of the present invention. Any equivalent structural or procedural transformations made based on the content of the present invention specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of the present invention.

Claims

1. A device for cultivating aseptic seedlings of alfalfa seeds, characterized in that, include: The components include a placement rack, light-shielding parts, a temperature control device, a lifting cylinder, multiple supplementary lights, multiple connecting mechanisms, and multiple fasteners. The placement rack includes a mounting frame, a top plate, and multiple support plates. The top plate is slidably mounted on the top of the mounting frame, and the multiple support plates are spaced apart and installed inside the mounting frame. Multiple supplementary lights are correspondingly installed at the bottom of the top plate and the bottom of the support plate; The light-shielding component is used to block the mounting frame and prevent natural light from entering the interior of the mounting frame; The temperature control device is mounted on the mounting frame and is used to regulate the temperature inside the mounting frame. The lifting cylinder is installed on the top of the mounting frame, and the output end of the lifting cylinder is connected to the top plate. Each bearing plate is detachably connected to the mounting frame through a fastener. The connecting mechanism includes a connecting arm and an assembly head. One end of the multiple connecting arms is installed on the bottom of the top plate and the bottom of the bearing plate, and the multiple assembly heads are installed on the other end of the corresponding connecting arm. The bearing plate is provided with a first assembly hole that is adapted to the assembly head. The fastener includes a square tube, a support block, a connecting shaft, and an end cap. The square tube is mounted on the bearing plate. One end of the support block is slidably disposed inside the square tube. One end of the connecting shaft passes through the square tube and is connected to the support block. The end cap is mounted on the other end of the connecting shaft. The mounting frame has multiple second mounting holes. The other end of the support block is inserted into a corresponding second mounting hole. The top surface of the support block is set as an inclined surface. The assembly head includes a movable cap, a support cap, and multiple driving blocks. The multiple driving blocks are arranged around the connecting arm and are rotatably connected to the connecting arm. The support cap is installed at the bottom end of the connecting arm, and the movable cap is sleeved on the connecting arm and located between the support cap and the driving blocks.

2. The aseptic seedling cultivation device for alfalfa seeds according to claim 1, characterized in that, The temperature control device includes a mounting plate and an exhaust fan. The mounting plate is mounted on the mounting frame, and the exhaust fan is mounted on the mounting plate.

3. The aseptic seedling cultivation device for alfalfa seeds according to claim 1, characterized in that, The alfalfa seed sterile seedling cultivation device also includes multiple reflective tin foils, which are installed on the top of each of the support plates.

4. The aseptic seedling cultivation device for alfalfa seeds according to claim 3, characterized in that, The top of the support plate has a placement groove, and the reflective tin foil is placed inside the placement groove.

5. The aseptic seedling cultivation device for alfalfa seeds according to claim 1, characterized in that, The mounting frame also includes a slide bar, the bottom end of which is mounted on the mounting frame, and the top end of which passes through multiple load-bearing plates and a top plate in sequence.

Images