A compound functional product with aphrodisiac effect

Abstract

The application provides a compound functional product with the effect of invigorating yang, which is composed of the following components in parts by weight: 1-3 parts of radix puerariae, 1.5-2.5 parts of rhizoma polygonati, 0.1-0.5 parts of cordyceps militaris, 0.1-1 parts of dioscorea, and 0.1-0.2 parts of mulberry; the above-mentioned formula has a new use in the preparation of health care for invigorating yang, and lays a foundation for developing medicines with the effect of invigorating yang; the crude extract is obtained from radix puerariae, rhizoma polygonati, cordyceps militaris, dioscorea and mulberry, and through compounding and functional evaluation, the extracted components and formula with the effect of invigorating yang are obtained; the formula has the effect of improving the kidney yang deficiency type of impotence through the researches of the gripping force and autonomous activity test, the sperm quantity test, the penile tissue cyclic adenosine monophosphate, cyclic guanosine monophosphate, nitric oxide and nitric oxide synthase level test and the like.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, specifically to a compound functional product with aphrodisiac effects. Background Technology

[0002] Erectile dysfunction (ED), also known as impotence, refers to a condition where the ability to achieve and maintain an erection is reduced to the point of being insufficient for satisfactory sexual intercourse. While it primarily affects men over 40, its prevalence is increasing among younger men due to rising life pressures and other factors. Social progress and rapid economic growth have led to a greater focus on quality of life. Therefore, effective treatment of ED has become a pressing issue that needs to be addressed.

[0003] Traditional Chinese medicine (TCM) believes that sufficient blood flow is the physiological basis for erection, and kidney yang deficiency is the fundamental pathogenesis of impotence. TCM has conducted in-depth research on this condition and provided various treatment approaches. TCM treatment for mild to moderate functional impotence is significantly effective, and it plays a significant role in regulating the patient's overall symptoms. Therefore, this invention selects several Chinese medicinal herbs for combination and screens suitable formula ratios to lay the foundation for the development of subsequent products. Summary of the Invention

[0004] This invention provides a multifunctional product with aphrodisiac effects, aiming to solve the problems mentioned in the background art.

[0005] The present invention is achieved as follows: a compound functional product with aphrodisiac effects, which is composed of the following components in parts by weight: 1-3 parts of kudzu root, 1.5-2.5 parts of polygonatum rhizome, 0.1-0.5 parts of cordyceps militaris, 0.1-1 parts of yam, and 0.1-0.2 parts of mulberry.

[0006] Preferably, a compound functional product with aphrodisiac effects is composed of the following components: 2g of kudzu root, 1.75g ​​of polygonatum rhizome, 0.33g of cordyceps militaris, 0.5g of yam, and 0.13g of mulberry.

[0007] Preferably, the kudzu root is kudzu root diced from Jiangshan, Zhejiang.

[0008] Preferably, it includes the following steps:

[0009] S01: Pueraria lobata extract → Add ultrapure water at a material-to-liquid ratio of 1:10 (g / mL) and reflux for 1.5 h → Repeat twice → Filter after cooling → Combine the filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → Microwave vacuum dry → Pueraria lobata extract;

[0010] Polygonatum → Dry at 60℃ → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate the ethanol → Add ultrapure water at a material-to-liquid ratio of 1:20 (g / mL) → Extract in an 85℃ water bath for 2 h → Repeat twice → Filter after cooling → Combine the filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → Add 4 times the amount of 95% ethanol to the concentrate while stirring, in small amounts multiple times to avoid large aggregates (ethanol concentration in the system is 80%) → Let stand at 4℃ for 24 h → Discard the supernatant, and microwave vacuum dry the precipitate → Polygonatum extract;

[0011] Weigh Cordyceps militaris → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate the ethanol → Add ultrapure water at a material-to-liquid ratio of 1:30 (g / mL) → Soak in an 80℃ water bath for 2 h → Repeat twice → Filter after cooling → Combine the filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → Add 4 times the amount of 95% ethanol to the concentrate while stirring, in small amounts multiple times to avoid large aggregates → Let stand at 4℃ for 24 h → Discard the supernatant, and microwave vacuum dry the precipitate → Cordyceps militaris extract;

[0012] Mulberry → Dry at 60℃ → Weigh mulberries → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate ethanol → Add ultrapure water at a material-to-liquid ratio of 1:30 (g / mL) → Extract in a 90℃ water bath for 2 h → Repeat twice → Filter after cooling → Combine filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → After cooling to room temperature, add 4 times the amount of 95% ethanol to the concentrate while stirring, adding in small amounts multiple times to avoid large aggregates → Let stand at 4℃ for 24 h → Discard the supernatant, and microwave vacuum dry the precipitate → Mulberry extract;

[0013] Yam → Peel and slice → Weigh the yam → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate the ethanol → Add ultrapure water at a material-to-liquid ratio of 1:20 (g / mL) → Soak in a 55℃ water bath for 2.5 h → Repeat twice → Filter after cooling → Combine the filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → After cooling to room temperature, add 4 times the amount of 95% ethanol to the concentrate while stirring, adding in small amounts multiple times to avoid large aggregates → Let stand at 4℃ for 24 h → Discard the supernatant, and microwave vacuum dry the precipitate → Yam extract;

[0014] S02: The above extracts, namely kudzu root, polygonatum rhizome, cordyceps militaris, yam, and mulberry, are mixed in a ratio of 48.74:3.95:6.75:1.73:0.7.

[0015] Preferably, in step S01, the total flavonoid content of kudzu root extract is ≥10%, the polysaccharide content of polygonatum extract is ≥40%, the polysaccharide content of cordyceps extract is ≥50%, the polysaccharide content of mulberry extract is ≥50%, and the polysaccharide content of yam extract is ≥30%.

[0016] Preferably, in step S01, puerarin is added to ultrapure water at a material-to-liquid ratio of 1:10 (g / mL) and refluxed for 1.5 h, and the process is repeated twice.

[0017] Preferably, in step S01, the extraction conditions for Polygonatum sibiricum are: drying at 60℃, soaking in 6 times the amount of 95% ethanol to remove alcohol, followed by extraction in a water bath at 85℃ for 2 hours at a material-to-liquid ratio of 1:20 (g / mL), and repeated twice.

[0018] Preferably, in step S01, the extraction conditions for Cordyceps militaris are: soaking in 6 times the amount of 95% ethanol to remove alcohol, followed by extraction in a water bath at 80℃ for 2 hours at a material-to-liquid ratio of 1:30 (g / mL), and repeated twice.

[0019] Preferably, in step S01, the mulberry extraction conditions are: drying at 60℃, soaking in 6 times the amount of 95% ethanol to remove alcohol, and then extracting in a water bath at 90℃ for 2 hours at a material-to-liquid ratio of 1:30 (g / mL), repeated twice.

[0020] Preferably, in step S01, the yam extraction conditions are: drying at 60℃, soaking in 6 times the amount of 95% ethanol to remove alcohol, and then extracting in a water bath at 55℃ for 2.5 h at a material-to-liquid ratio of 1:20 (g / mL), repeated twice.

[0021] Due to the adoption of the above scheme, the beneficial effects of the present invention are: (1) The present invention has discovered a new use of the above formula in the preparation of aphrodisiac health care through experiments, laying the foundation for the development of drugs with aphrodisiac effects.

[0022] (2) The present invention extracts crude extracts from kudzu root, polygonatum, cordyceps militaris, yam and mulberry, and obtains extracts and formulas with good aphrodisiac effects through compounding and functional evaluation.

[0023] (3) Studies of this invention, including gripping force and spontaneous activity tests, sperm count tests, penile tissue cyclic adenosine monophosphate, cyclic guanosine monophosphate, nitric oxide and nitric oxide synthase level tests, etc., show that the formula has the effect of improving impotence of kidney yang deficiency type.

[0024] (4) The preparation method of the present invention is simple to operate and easy to industrialize; the raw materials used are inexpensive and abundant. Attached Figure Description

[0025] Figure 1 The effect of the formulation on the weight gain of mice ( ± SEM, n = 8);

[0026] Figure 2 Effect of formulation on the number of spontaneous activities in mice ± SEM, n = 8);

[0027] Figure 3 Effect of formulation on grip strength in mice ± SEM, n = 8);

[0028] Figure 4 Effect of formulation on mouse kidney index ( ± SEM, n = 8);

[0029] Figure 5 Effect of formulation on seminal vesicle index in mice ± SEM, n = 8);

[0030] Figure 6 Effect of formulation on epididymal index in mice ± SEM, n = 8);

[0031] Figure 7 Effect of formulation on mouse testicular index ( ± SEM, n = 8);

[0032] Figure 8 Effect of formulation on penile tissue index in mice ± SEM, n = 8);

[0033] Figure 9 Effect of formulation on sperm count in mice ± SEM, n = 8);

[0034] Figure 10 Effect of formulation on serum Cr levels in mice ± SEM, n = 8);

[0035] Figure 11 Effect of formulation on serum BUN levels in mice ± SEM, n = 8);

[0036] Figure 12 Effect of formulation on serum E2 levels in mice ± SEM, n = 8);

[0037] Figure 13 Effect of formulation on SOD levels in mouse kidneys ± SEM, n = 8);

[0038] Figure 14 Effect of formulation on GSH-Px levels in mouse kidneys ( ± SEM, n = 8);

[0039] Figure 15 Effect of formulation on MDA levels in mouse kidneys ± SEM, n = 8);

[0040] Figure 16 Effect of formulation on cAMP levels in mouse penile tissue ± SEM, n = 8);

[0041] Figure 17 Effect of formulation on cGMP levels in mouse penile tissue ( ± SEM, n = 8);

[0042] Figure 18 Effect of formulation on cAMP / cGMP levels in mouse penile tissue ± SEM, n = 8);

[0043] Figure 19 Effect of formulation on NO levels in mouse penile tissue ( ± SEM, n = 8);

[0044] Figure 20 Effect of formulation on NOS levels in mouse penile tissue ± SEM, n = 8). Detailed Implementation

[0045] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0046] A compound functional product with aphrodisiac effects is composed of the following components in parts by weight: 1-3 parts kudzu root, 1.5-2.5 parts polygonatum rhizome, 0.1-0.5 parts cordyceps militaris, 0.1-1 parts yam, and 0.1-0.2 parts mulberry.

[0047] Specifically, 1. Extract preparation,

[0048] The specific steps are as follows:

[0049] Pueraria lobata extract → Add ultrapure water at a material-to-liquid ratio of 1:10 (g / mL) and reflux for 1.5 h → Repeat twice → Filter after cooling → Combine the filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → Microwave vacuum drying → Pueraria lobata extract.

[0050] Polygonatum → Dry at 60℃ → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate the ethanol → Add ultrapure water at a material-to-liquid ratio of 1:20 (g / mL) → Extract in an 85℃ water bath for 2 h → Repeat twice → Filter after cooling → Combine the filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → Add 4 times the amount of 95% ethanol to the concentrate while stirring, in small amounts multiple times to avoid large aggregates (ethanol concentration in the system is 80%) → Let stand at 4℃ for 24 h → Discard the supernatant, and microwave vacuum dry the precipitate → Polygonatum extract.

[0051] Weigh Cordyceps militaris → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate the ethanol → Add ultrapure water at a material-to-liquid ratio of 1:30 (g / mL) → Extract in an 80℃ water bath for 2 h → Repeat twice → Filter after cooling → Combine the filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → Add 4 times the amount of 95% ethanol to the concentrate while stirring, in small amounts multiple times to avoid large aggregates (ethanol concentration in the system is 80%) → Let stand at 4℃ for 24 h → Discard the supernatant, and microwave vacuum dry the precipitate → Cordyceps militaris extract.

[0052] Mulberry → Dry at 60℃ → Weigh mulberries → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate ethanol → Add ultrapure water at a material-to-liquid ratio of 1:30 (g / mL) → Extract in a 90℃ water bath for 2 h → Repeat twice → Filter after cooling → Combine filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → After cooling to room temperature, add 4 times the amount of 95% ethanol to the concentrate while stirring, in small amounts multiple times to avoid large aggregates (ethanol concentration in the system is 80%) → Let stand at 4℃ for 24 h → Discard the supernatant, precipitate and microwave vacuum dry → Mulberry extract.

[0053] Yam → Peel and slice → Weigh yam → Soak in 6 times the amount of 95% ethanol for 24 h → Evaporate ethanol → Add ultrapure water at a material-to-liquid ratio of 1:20 (g / mL) → Soak in a 55℃ water bath for 2.5 h → Repeat twice → Filter after cooling → Combine filtrates → Concentrate the filtrate using a rotary evaporator → Concentrate to 1 / 4 of the original solution volume → After cooling to room temperature, add 4 times the amount of 95% ethanol to the concentrate while stirring, in small amounts multiple times to avoid large aggregates (ethanol concentration in the system is 80%) → Let stand at 4℃ for 24 h → Discard the supernatant, and microwave vacuum dry the precipitate → Yam extract.

[0054] Formula ratio,

[0055] The above extracts were formulated in a ratio of 48.74:3.95:6.75:1.73:0.7 (kudzu root: polygonatum: cordyceps: yam: mulberry), with a raw material ratio of 2:1.75:0.33:0.5:0.13.

[0056] 2. Determination of total flavonoids and polysaccharide content.

[0057] Standard curve creation

[0058] (1) Accurately pipette 0, 20, 40, 60, 80, 100, 120, and 140 μL of rutin standard solution, equivalent to 0, 3, 6, 9, 12, 15, 18, and 21 μg of rutin, into 2.5 mL volumetric flasks. Add 100 μL of 5% NaNO2 solution, shake, and let stand for 6 min. Add 100 μL of 10% Al(NO3)3 solution, shake well, and let stand for 6 min. Add 1.0 mL of 4% NaOH solution, shake well, and let stand for 15 min. Add 70% ethanol to each test tube to bring the volume to 2.5 mL. Pipette 200 μL of the reaction solution into a 96-well plate, making 3 replicates per group. Measure the absorbance at 510 nm using a microplate reader. Finally, plot a standard curve with absorbance as the ordinate and standard concentration as the abscissa to obtain the regression equation.

[0059] (2) Using a pipette, pipettes 0, 25, 50, 75, 100, 125, 150, and 175 μL of glucose standard solution into clean, dry test tubes. Distilled water is added to each tube to a final volume of 250 μL. Then, 150 μL of 6% phenol is added to each tube, followed by a slow addition of 800 μL of concentrated sulfuric acid (while shaking). The tubes are allowed to stand at room temperature for 30 min. 200 μL of the reaction solution is then pipetteed into a 96-well plate, with three replicates per group. The absorbance is measured at 490 nm using a microplate reader, with distilled water as a blank. Finally, a standard curve is plotted with absorbance as the ordinate and standard concentration as the abscissa to obtain the regression equation.

[0060] Sample determination: Take the sample and test its absorbance according to the standard determination procedure. Calculate the content of the corresponding components according to the corresponding standard curve.

[0061] 3. Animal experimental methods,

[0062] Modeling, grouping, and drug administration

[0063] Ninety male ICR mice were randomly divided into four groups (n=15 per group) after 7 days of acclimatization feeding: a normal control group, a model group, a Jin Kui Shen Qi Wan (1.7 g / kg) group, and low, medium, and high dose groups (1.3, 1.5, and 1.9 g / kg, respectively). The normal control and model groups were administered an equal volume of distilled water by gavage, while the other groups received the corresponding drug via gavage for 14 consecutive days. Except for the normal control group, the other groups received intraperitoneal injections of 12.5 mg / kg hydrocortisone solution daily from day 15 to day 17 to establish a kidney-yang deficiency mouse model. The normal control group received an equal volume of physiological saline solution in the same manner. After model establishment, the normal control and model groups continued to receive an equal volume of distilled water by gavage, while the other groups received the corresponding drug solution via gavage for 4 consecutive days.

[0064] The criteria for successfully establishing a kidney-yang deficiency model are: mice exhibiting symptoms similar to kidney-yang deficiency, such as aversion to cold, huddling together, slowed weight gain, sparse and dull body hair, and fatigue (reduced grip strength and fewer spontaneous movements).

[0065] Detection methods and indicators

[0066] (1) Morphological observation,

[0067] During the experiment, the mice were weighed and recorded every 3 days to observe whether they exhibited a series of symptoms similar to those of kidney yang deficiency, such as aversion to cold and preference for warmth, huddling together and inactive, lethargy, emaciation, and sparse, messy, and dull body hair.

[0068] (2) Measurement of the gripping strength and number of spontaneous movements of mice.

[0069] Three hours after the last drug administration, the grip strength and spontaneous activity of the mice were measured. Grip strength was measured using a grip strength meter. The meter was placed on a horizontal table, and the mouse was gently placed on the grip plate. After the mouse had a firm grip, its tail was grasped and slowly and evenly pulled backward. The value at which the meter emitted a sound was recorded as the mouse's maximum grip strength. Each mouse was measured three times, and the average value was taken. Spontaneous activity was measured in a quiet, dimly lit environment in the afternoon. The mice were placed in a spontaneous activity box, and after acclimatizing for 5 minutes, the number of movements of the mice in the following 10 minutes was recorded.

[0070] (3) Determination of organ indices,

[0071] After the last administration, the mice were fasted for 12 hours but allowed to drink water. They were weighed, and the kidneys, seminal vesicles, epididymis, testes, and penis tissues of the mice were carefully separated and weighed using an electronic balance. The organ weights were recorded, and the organ coefficients were calculated [organ coefficient = organ weight / body weight × 100%].

[0072] (4) Sperm count determination,

[0073] The mouse epididymis was isolated and placed in 10 mL of sterile phosphate buffer. It was then cut into small pieces to release the sperm into the buffer. The mixture was then incubated in a 37°C incubator for 30 min. 10 μL of the buffer was then added to a cell counting chamber, and the number of sperm in the epididymis was counted using a LUNA-FL dual-fluorescence cell counter.

[0074] (5) Detection of serum biochemical indicators,

[0075] After blood collection, the blood samples were left to stand for 2 hours, then centrifuged at 3500 rpm for 10 min. The serum was collected and stored at -80℃ for later use. Serum Cr and BUN levels were determined according to the kit instructions. E2 levels in mouse serum were detected using an ELISA kit.

[0076] (6) Detection of renal oxidative indicators,

[0077] Kidneys were harvested and added to physiological saline at a ratio of 1:9. The mixture was homogenized using a tissue homogenizer, lysed on ice for 30 min, and centrifuged at 12,000 rpm for 10 min at 4°C. The supernatant was collected, and the activities of SOD, GSH-Px, and MDA were measured according to the kit instructions.

[0078] (7) Detection of cAMP, cGMP, NO, and NOS in penile tissue.

[0079] Mouse penile tissue was collected, rinsed with physiological saline, and then homogenized with physiological saline at a ratio of 1:9 using a tissue homogenizer. The homogenate was lysed on ice for 30 min, centrifuged at 12,000 rpm for 10 min at 4°C, and the supernatant was collected. The contents of cAMP, cGMP, NO, and NOS in the penile tissue were determined according to the kit instructions, and the cAMP / cGMP value was calculated.

[0080] (8) Statistical analysis of data,

[0081] GraphPad 8.0 software was used for statistical analysis of the data. Data are expressed as mean ± SEM, and one-way ANOVA was used for comparisons among multiple groups. A p-value < 0.05 was considered statistically significant.

[0082] 4. Results of animal experiments

[0083] (1) Content of flavonoids and polysaccharides in the extract,

[0084] As shown in Table 1, the total flavonoid content of kudzu root was 11.35% ± 0.86%, the crude polysaccharide content of polygonatum rhizome was 48.96% ± 5.05%, the crude polysaccharide content of cordyceps militaris was 60.54% ± 4.60%, the crude polysaccharide content of mulberry fruit was 59.05% ± 5.35%, and the crude polysaccharide content of yam was 34.78% ± 4.98%.

[0085] Table 1. Flavonoid and polysaccharide content in the extract (± SEM, n = 3)

[0086]

[0087] (2) Mouse weight gain,

[0088] like Figure 1 As shown (Note: Compared with the normal group, ##P < 0.01; compared with the model group, **P < 0.01). Three days after hydrocortisone-induced modeling, mice exhibited symptoms such as aversion to cold, huddling together with little movement, lethargy, slow movement, and dull fur. Compared with the normal group, their weight gain was significantly slower (P < 0.01), indicating that the mice had kidney yang deficiency syndrome. After administration, compared with the model group, the mice in each administration group showed significant improvement in condition and significantly increased weight gain (P < 0.01), indicating that the formulation composed of extracts from five traditional Chinese medicines—kudzu root, polygonatum rhizome, cordyceps militaris, mulberry fruit, and yam—had an ameliorative effect on hydrocortisone-induced kidney yang deficiency syndrome in mice.

[0089] (3) Number of autonomous activities and grip strength value,

[0090] like Figure 2 , Figure 3 As shown in the figure. Compared with the normal group, the number of spontaneous movements and the gripping force of mice in the model group were significantly decreased (P < 0.01); compared with the model group, the number of spontaneous movements and the gripping force of mice in each drug administration group were significantly increased (P < 0.01), and the low, medium and high dose groups of the formulation showed obvious dose dependence.

[0091] (4) Effects of the formulation on organ indices in mice.

[0092] like Figures 4-8 As shown in the figure. Compared with the normal group, the kidney index of mice in the model group was significantly increased (P < 0.01), while the seminal vesicle index, epididymal index, testicular index, and penile tissue index were all significantly decreased (P < 0.01). Compared with the model group, the seminal vesicle index, epididymal index, testicular index, and penile tissue index were all significantly increased (P < 0.05, P < 0.01). Meanwhile, the kidney index of the medium-dose group and the high-dose group of the formulation were significantly decreased (P < 0.05, P < 0.01).

[0093] (5) Effect of the formulation on sperm count in mice,

[0094] like Figure 9As shown in the figure, compared with the normal group, the sperm count of mice in the model group was significantly reduced (P < 0.01), indicating that the mouse model of kidney yang deficiency was successfully established. Compared with the model group, the sperm count of mice in different dosage groups of the formula was significantly increased (P < 0.05, P < 0.01), and showed obvious dose dependence, indicating that the formula can improve the kidney yang deficiency syndrome caused by hydrocortisone to a certain extent and has an aphrodisiac effect.

[0095] (6) Effects of the formulation on serum creatinine, blood urea nitrogen and estradiol levels in mice.

[0096] Serum creatinine (Cr) and blood urea nitrogen (BUN) levels reflect glomerular filtration and are important indicators for assessing kidney function. Estradiol (E2) is an objective indicator for evaluating kidney yang deficiency. Figures 10-12 As shown, compared with the normal group, the serum levels of Cr, BUN and E2 in the model group mice were significantly increased (P < 0.01); compared with the model group, the serum levels of Cr, BUN and E2 in each drug administration group mice were significantly decreased (P < 0.01).

[0097] (7) Effects of the formulation on the levels of superoxide dismutase, glutathione peroxidase and malondialdehyde in mouse kidneys.

[0098] Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) are indicators of antioxidant capacity. Figures 13-15 As shown in the figure, compared with the normal group, the activities of SOD and GSH-Px in the kidney tissue of the model group mice were significantly decreased (P < 0.01), and the MDA level was significantly increased (P < 0.01), indicating that hydrocortisone caused the mouse kidney tissue to be in a state of oxidative stress damage. Compared with the model group, all different drug administration groups could enhance the activities of SOD and GSH-Px and reduce the MDA level in the mouse kidney tissue (P < 0.05, P < 0.01), suggesting that the formula can alleviate the kidney function damage in mice with kidney yang deficiency by improving the antioxidant stress capacity of mice.

[0099] (8) Effects of the formulation on the levels of cyclic adenosine monophosphate, cyclic guanosine monophosphate, nitric oxide, and nitric oxide synthase in mouse penile tissue.

[0100] Kidney Yang deficiency syndrome is characterized by dysfunction of multiple target glands (hypothalamic-pituitary-adrenal axis, thyroid axis, and gonadal axis). Cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), as second messengers in the body, mediate signal transduction of neurotransmitters and endocrine hormones, exerting their biological effects. For example... Figures 16-18As shown in the figure, compared with the normal group, the cAMP content and cAMP / cGMP ratio in the penile tissue of the model group mice were significantly decreased (P < 0.01), while the cGMP level was significantly increased (P < 0.01), indicating that the cyclic nucleotide system reactivity in the kidney-yang deficiency mice was imbalanced, which is a manifestation of the inhibition of the adrenal cortex system function. Compared with the model group, different dosage groups of the formula significantly increased the cAMP content and cAMP / cGMP value and decreased the cGMP level (P < 0.05, P < 0.01).

[0101] Nitric oxide (NO), as a signaling substance in the central and peripheral nervous systems, can be released by activating nitric oxide synthase (NOS) in the corpora cavernosa, causing vasodilation, engorgement of the corpora cavernosa, and inducing penile erection. Experimental results show ( Figure 19 , Figure 20 After hydrocortisone intervention, the levels of NO and NOS in the model group mice were significantly reduced (P < 0.01). The medium-dose and high-dose groups of the formula increased the levels of NO and NOS (P < 0.01), suggesting that the formula can promote the synthesis and release of NO and NOS by regulating the neuroendocrine immune system, thereby improving the symptoms of kidney yang deficiency. In summary, this experiment used hydrocortisone to replicate a mouse model of kidney yang deficiency. After modeling, the mice exhibited obvious "signs of yang deficiency," such as weight loss, dry and dull fur, lethargy, arched back and curled-up posture, aversion to cold and huddling together, loose stools, and reduced spontaneous activity. After administering a formula composed of extracts from five traditional Chinese medicines—kudzu root, polygonatum, cordyceps militaris, mulberry, and yam—it could significantly improve the kidney yang deficiency syndrome induced by hydrocortisone in mice, improve renal function damage in the model mice, and exert the effect of tonifying the kidney and strengthening yang.

[0102] The above description of the embodiments is intended to enable those skilled in the art to understand and use the present invention. It will be apparent to those skilled in the art that various modifications can be made to these embodiments, and the general principles described herein can be applied to other embodiments without inventive effort. Therefore, the present invention is not limited to the above embodiments. Improvements and modifications made by those skilled in the art based on the principles of the present invention without departing from the scope of the invention should be within the protection scope of the present invention. The above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A multifunctional product with aphrodisiac effects, characterized in that, It is prepared from the following raw materials in the following parts by weight using the following preparation process: The ingredients are: 1-3 parts kudzu root, 1.5-2.5 parts polygonatum, 0.1-0.5 parts cordyceps militaris, 0.1-1 part yam, and 0.1-0.2 parts mulberry. The preparation process includes the following steps: S01: Pueraria lobata extract was extracted by reflux with ultrapure water at a material-to-liquid ratio of 1:10 g / mL for 1.5 h, repeated twice. After cooling, the extract was filtered and the filtrates were combined. The filtrates were concentrated to 1 / 4 of the original solution volume using a rotary evaporator and dried under microwave vacuum to obtain pueraria lobata extract. After drying at 60℃, Polygonatum was soaked in 6 times its volume of 95% ethanol for 24 h. The ethanol was evaporated, and then ultrapure water was added at a material-to-liquid ratio of 1:20 g / mL. The mixture was then extracted in an 85℃ water bath for 2 h, and this process was repeated twice. After cooling, the mixture was filtered, and the filtrates were combined. The filtrate was concentrated to 1 / 4 of the original solution volume using a rotary evaporator. 4 times its volume of 95% ethanol was added to the concentrate while stirring, in small amounts several times to avoid large aggregates. The mixture was allowed to stand at 4℃ for 24 h, and the supernatant was discarded. The precipitate was dried under microwave vacuum to obtain Polygonatum extract. Cordyceps militaris was soaked in 6 times its volume of 95% ethanol for 24 hours, the ethanol was evaporated, and then ultrapure water was added at a solid-liquid ratio of 1:30 g / mL. The mixture was then extracted in an 80℃ water bath for 2 hours, and this process was repeated twice. After cooling, the mixture was filtered, and the filtrates were combined. The filtrate was concentrated to 1 / 4 of the original solution volume using a rotary evaporator. 4 times its volume of 95% ethanol was added to the concentrate while stirring, in small amounts several times to avoid the formation of large aggregates. The mixture was allowed to stand at 4℃ for 24 hours, the supernatant was discarded, and the precipitate was dried under microwave vacuum to obtain the Cordyceps militaris extract. After drying the mulberries at 60℃, they were soaked in 6 times the amount of 95% ethanol for 24 hours. The ethanol was evaporated, and then ultrapure water was added at a material-to-liquid ratio of 1:30 g / mL. The mixture was then extracted in a 90℃ water bath for 2 hours, and this process was repeated twice. After cooling, the mixture was filtered, and the filtrates were combined. The filtrate was concentrated to 1 / 4 of the original solution volume using a rotary evaporator. After cooling to room temperature, 4 times the amount of 95% ethanol was added while stirring, in small amounts several times to avoid the formation of large aggregates. The mixture was allowed to stand at 4℃ for 24 hours, and the supernatant was discarded. The precipitate was dried under microwave vacuum to obtain the mulberry extract. After peeling and slicing the yam, it was soaked in 6 times the amount of 95% ethanol for 24 hours. The ethanol was evaporated, and then ultrapure water was added at a solid-liquid ratio of 1:20 g / mL. The mixture was then extracted in a 55℃ water bath for 2.5 hours. This process was repeated twice. After cooling, the mixture was filtered, and the filtrates were combined. The filtrate was concentrated to 1 / 4 of the original solution volume using a rotary evaporator. After cooling to room temperature, 4 times the amount of 95% ethanol was added while stirring, in small amounts several times to avoid the formation of large aggregates. The mixture was allowed to stand at 4℃ for 24 hours. The supernatant was discarded, and the precipitate was dried under microwave vacuum to obtain the yam extract. S02: The obtained kudzu root extract, polygonatum extract, cordyceps extract, yam extract, and mulberry extract are mixed in a mass ratio of 48.74:3.95:6.75:1.73:0.7 to obtain the composite functional product.

2. The compound functional product with aphrodisiac effects according to claim 1, characterized in that, The amounts of the raw materials used are: 2g of kudzu root, 1.75g ​​of polygonatum rhizome, 0.33g of cordyceps militaris, 0.5g of yam, and 0.13g of mulberry.

3. The compound functional product with aphrodisiac effects according to claim 2, characterized in that, The kudzu root mentioned is kudzu root diced from Jiangshan, Zhejiang.

4. The compound functional product with aphrodisiac effects according to claim 1, characterized in that: The total flavonoid content of kudzu root extract is ≥10%, the polysaccharide content of polygonatum extract is ≥40%, the polysaccharide content of cordyceps extract is ≥50%, the polysaccharide content of mulberry extract is ≥50%, and the polysaccharide content of yam extract is ≥30%.

Images