一种肝脏脱细胞基质的制备方法
By employing a freeze-thaw elution method combined with SDS, Triton X-100, and D-Hank's-EDTA solutions, the problem of difficult removal of hepatic vascular and bile duct terminal cells was solved, achieving efficient preparation of decellularized liver matrix while preserving the structural and functional components of the liver.
CN118576775BActive Publication Date: 2026-07-17FIRST AFFILIATED HOSPITAL OF XINJIANG MEDICAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- FIRST AFFILIATED HOSPITAL OF XINJIANG MEDICAL UNIVERSITY
- Filing Date
- 2024-05-21
- Publication Date
- 2026-07-17
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Figure CN118576775B_ABST
Abstract
本发明涉及生物材料与组织工程技术领域，具体涉及一种肝脏脱细胞基质的制备方法，包括：将清洗的新鲜肝脏冷冻；将冷冻后的肝脏进行一次洗脱；将一次洗脱的肝脏清洗后进行二次洗脱；将二次洗脱的肝脏清洗后进行三次洗脱，即可。本发明的肝脏脱细胞基质的制备方法可剥离肝脏后批量脱细胞操作，不用穿刺动脉也不用持续观察；低温冷冻处理后肝脏不恢复室温，直接置于脱细胞液中利用温度急剧降低过程形成冰晶破坏细胞膜，不解冻即放入脱细胞液中促进细胞破裂利于脱去细胞。
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